1. sports
2. diving

Komentář [l1]: H
Komentář [l2]: U
Komentář [L7]: 正确的称... [1]
Odstraněno: Table of ... [2]
Contents¶
Komentář [l3]: 是否加 of
Komentář [l5]: 删除
Komentář [l6]: 的
Odstraněno: 45 年前也就是
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Odstraněno: 荷兰阿姆斯特
... [4]
Odstraněno: 请
Odstraněno: 各国同仁积极
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Odstraněno:
Odstraněno: 了最初被 Ite
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Odstraněno: 纯
Odstraněno: 氧浸泡
Invited Lecture
Odstraněno: 透法
Odstraněno: ”的高压氧疗法
Odstraněno:
Odstraněno: 在不同病种中
... [7]
大会发言
Odstraněno: 用于不同症状
... [8]
Odstraněno: 。¶
Odstraněno: ，“高压纯氧浸
... [9]
Odstraněno: 当时高压氧疗
... [10]
Odstraněno: 用途是
Odstraněno: 适应症为以厌
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Odstraněno: 学
Odstraněno: 疗
Odstraněno: 法
Odstraněno: 和高温疗法治
... [12]
Odstraněno: 各种传染病，例
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Odstraněno: 应用高压氧治
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Odstraněno: ，科研和临床
... [15]
Odstraněno: 同时也对
Odstraněno: 与研究
Odstraněno: 这种新疗法的
Odstraněno: 同时，大家还也
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Odstraněno: 生理、药理以及
... [17]
Odstraněno: 的
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B1
The use of Hyperbaric Oxygen in Necrotising Soft Tissue Infections: A Historical Perspective with an Update
anno 2008
D. J. Bakker, MD, PhD.
Amsterdam, the Netherlands
Introduction:
Necrotizing soft tissue infections caused by aerobic, anaerobic and mixed bacterial floras are a still increasing problem in
surgical and medical practice. They occur with increasing frequency and seriousness, especially in immune-compromised
patients.
The clinical picture can vary considerably from patient to patient. Treatment is difficult, often irrational and very often "one
step behind the facts," because early recognition is difficult and etiology, bacteriology, and the clinical course are
sometimes not well understood or expected to evolve in a different and more favourable way. Hyperbaric Oxygen
treatment (HBOT) is a highly controversial issue in the literature.
Considerable morbidity occurs in these diseases and the mortality can be very high, from 20% up to 70 or 80%.
Odstraněno: aetiology
History:
For a proper understanding it is necessary to give a short review of the history of these infections including the
bacteriology and the various forms of treatment that have been advocated and practised.
th
In this review we will pay attention to the works of Hippocrates from the 5 century BC, Baurienne (1764), Hebler (1848)
and Jean Alfred Fournier (1883), a French venereologist,
Also the work by the Confederate Army Surgeon Joseph Jones will be mentioned who described a variant of this disease
in 1869 and 1870 during the Civil War in the United States.
Very relevant are also the works of Meleney and Cullen (1924) en Brewer and Meleney (1926). Meleney reported about
his work in the Imperial Hospital In Peking (now Beijing) in China.
Extensive reviews on necrotizing soft tissue infections including Fournier’s gangrene have been published and can be
found in the literature (Bakker 2002, Bakker 2008).
The immense confusion in the nomenclature and the classification of these diseases will be explained.
The role of Hyperbaric Oxygen in the treatment of these disease started in 1959 when Boerema and Brummelkamp
treated a patient with Gas Gangrene in the Hyperbaric Chamber (HBOT). The first Congress on HBOT was held in
Amsterdam in 1963, now 45 years ago.
Material and Methods:
We will restrict ourselves in this presentation to Clostridial Myonecrosis or Gas Gangrene and Necrotising Fasciitis or
Fourniers Gangrene.
a. Necrotising Fasciitis (NF) is a progressive, generally rapid spreading, inflammatory process located in the deep fascia
with secondary necrosis of subcutaneous tissues and skin. The infection tends to spread very rapidly along the deep
fascial planes. There is confusion in the literature about the microorganisms that cause NF. Haemolytic Streptococci play
a major role in the onset of the disease. Later on in the disease one can find many more microorganisms, from which their
role in the disease is not clear. A rapid diagnosis is essential for treatment and prognosis. Treatment is a combination of
proper, early and extensive surgery, antibiotics and adjunctive HBOT. The grade of evidence for using HBOT in the
treatment will be shown from the literature data and our own experiences.
b. Clostridial Myonecrosis or Gas Gangrene is an acute, rapidly progressive, non-pyogenic, gasforming and necrotising
infection of muscles, subcutaneous tissues and skin. The infection is caused by anaerobic spore-forming bacteria of the
genus Clostridium. Although the prognosis of the disease is improved by modern antibiotics and intensive care treatment,
the mortality and morbidity is still high, especially without HBOT. A rapid diagnosis has to be made, immediately followed
by HBOT, not losing any time by performing initial surgery. The patients are too ill for surgery. Treatment consists of
immediate HBOT and antibiotics, followed by, if necessary, surgical measures secondarily. This treatment has proven its
use in several thousands of patients treated in this way, with constant good results over the years.
Here also an update will be give about the best treatment modality anno 2008 and the evidence based reasons for that
from the literature and from our own results.
c. Evidence based medicine in HBOT (EBHM) is the combination of old and trusted clinical skills completed by and if
necessary also corrected by the critically appraised best available scientific research results and the evaluation of care.
The idea is to move beyond clinical experience and physiological principles to rigorous evaluations of the consequences
of clinical actions. These evaluations are in too many indications in hyperbaric medicine, non-existent. When looking for
the evidence of HBOT in infectious diseases several steps have to be taken. First of all the clinical problem has to be
appropriately formulated and the correct diagnosis has to be made. Therefore clinical expertise is required. Nothing can
Odstraněno: sthese
diseases
substitute for clinical expertise in determining the specific considerations relevant to the individual patient. Clinical skills
are necessary for EBHM plus an understanding of how the patients values affect the balance between advantages and
disadvantages of the available treatment options, and the ability to appropriately involve the patient in the decision. Every
decision taken on the basis of education and experience should, however, also be based on results of trials and
evaluations. In this way we can define HBOT as a primary mode of treatment or as an adjunctive mode. We must be
careful not to move the focus of clinical practice away from the care of individuals to the care of populations, lacking than
the ability to apply this knowledge back to our individual patient. The Randomised Controlled Clinical Trial (RCT) is
nowadays the gold standard in EBHM, but we must not forget that there is more evidence than that, that has to be taken
into account when judging HBOT.
Conclusion:
After a short description of soft tissue infections in the history of medicine, the state of the art in diagnosis and treatment of
some special forms of soft tissue infections, eg. necrotising fasciitis and clostridial myonecrosis is shown.
Especially the evidence based character of treatment with Hyperbaric oxygen based on own experiences and reports from
the literature is described.
B2
Role of Tissue Oximetry in Assessment of Problem Wounds
Paul J. Sheffield, PhD;
International ATMO, Inc, Nix Wound Healing Center, San Antonio, Texas, USA
Transcutaneous oximetry (PtcO2) is commonly used to determine treatment options for problem wounds: surgical remedy,
adjunctive hyperbaric oxygen (HBO2), or standard wound care. HBO2 wound healing candidates are selected by
assessing whether the wound area is hypoxic and if it will respond to respired oxygen. There are five PtcO2 tests
commonly used to evaluate wound patients.
1. Evaluate whether wound healing is complicated by severe hypoxia. Test 1: Measure a PtcO2 baseline air value at 1 atm
abs. Hypoxia exists if PtcO2 is less than 40 mmHg. The lower the PtcO2 value, the more severe is the hypoxia.
2. Evaluate whether wound healing is complicated by peripheral arterial occlusive disease. Test 2: Elevate the leg (30˚).
Disease is present if PtcO2 remains diminished more than 10 mmHg while leg is elevated.
3. Evaluate whether the wound area responds to respired O2. Test 3: Conduct an oxygen challenge at 1 atm abs. Values
on respired oxygen should reach at least 35 mmHg and should rise at least 50% above air PtcO2 values.
4. Evaluate whether the wound area responds to respired HBO2. Test 4 (Alternate to Test 3): Conduct an oxygen
challenge at 2-2.5 atm abs. Values on HBO2 should be at least 100 mmHg (preferably 200 mmHg).
5. Evaluate whether the wound is to the point where it will heal without further HBO2 treatment. Test 5: Repeat PtcO2
baseline air value at 1 atm abs in 3-4 wk intervals. Normalized baseline values indicate that healing process is in place.
PL 1
The Indication of Hyperbaric Oxygen Therapy and for Orthopaedics in Japan
Mahito Kawashima, Takashi Yamaguchi
Kawashima Orthopaedic Hospital, Japan
In Japan, two types of standards of indication of Hyperbaric Oxygen Therapy (HBO) are set. One is established by
Japanese Society of Hyperbaric and Undersea Medicine (JSHUM) and another is by the Ministry of Health, Labor and
Welfare for public medical insurance. Most cases are treated with the standard of the Ministry of Health, Labor and
Welfare, because of the medical serv. system for the whole nation in Japan. This standard, the disease is classified into
two types, emergency/non-emergency. The fee for treatment is different between emergency and non-emergency,
multiplace chamber and monoplace chambers. In the case of multiplace chamber, the fee for emergency is \60,000
( US$545) per usage time of HBO. In the case of monoplace chamber, the fee for emergency is \50,000 ( US$454) per
usage of HBO. The fee for non-emergency is \2,000 ( US$18) par usage of HBO, in the case of non-emergency the fee is
no different, multiplace or monoplace chambers.
The standard of indication of HBO by the Ministry of Health, Labor and Welfare is as following.
Emergency: Acute carbon monoxide poisoning, Gas poisoning, Air gas embolism, Decompression illness, Acute
peripheral vessel trouble, Thermal burns, Frostbite, Crush injury, Peripheral vessel trouble by trauma, Shock, Acute
myocardial infarction, Cerebral embolism, Severe head injury, Disturbance of consciousness or brain edema after
craniotomy, Hypoxic brain dysfunction, Intestinal atresia, occlusion of retinal artery, Sudden deafness, Acute spinal cord
injury.
Non emergency: Malignant tumor with carcinostatic or radiation therapy, Refractory ulcer, Skin graft, SMON,
Cerebrovascular disorder, Severe head injury, Motor paralysis after craniotomy, sequelae of carbon monoxide poisoning,
Spinal nerve disorder, Osteomyelitis, Radiation necrosis.
According to the research on March 2008, 51 multiplace chambers and 805 monoplace chambers have been working in
644 facilities in Japan.
Kawashima Orthopaedic Hospital specializes in orthopaedic surgery, has 93 beds for admission and two multiplace
hyperbaric chambers. The one has worked since 1984 and the other has worked since 1989. Kawashima Clinic is
juxtaposition clinic of Kawashima Orthopaedic Hospital. A multiplace hyperbaric chamber has been there since April
2005.
The main indications of HBO in orthopaedic surgery are Gas gangrene, Acute spinal cord injury, Crush injury,
Osteomyelitis and Refractory ulcer. HBO is expected for these disorders as inhibition and prevention for infection,
promotion of recover of damaged cells, promotion of angiogenesis and improvement of edema. The total number of cases
is 7,324 cases from 1981 to 2007 in Kawashima Orthopaedic Hospital. In these, Main orthopaedic disorders are 630
osteomyelitis, 183 crush injury, 76 spiral cord injury, 41 gas gangrene and 375 ulcer/necrosis.
PL2
The Use of “Deep Stops” In Preventing Precordial Detectable Doppler Bubbles in Recreational Scuba Divers
1
2
3
Frans J. Cronjé, MBChB, MSc , Alessandro Marroni, MD , Peter Bennett, DSc, PhD
1. DAN Southern Africa;
2. DAN Europe;
3. International DAN
Decompression illness (DCI) affects some 1500 divers every year. Although DCI is relatively rare, two thirds of these
divers develop neurological manifestations.
The empirical use of additional deep safety stops by recreational divers has become popular in recent years. However,
these arbitrary procedures have not been evaluated for safety and effectiveness.
In order to establish appropriate and practical recommendations relevant to typical recreational divers, a series of studies
were designed to evaluate the effect of adding deep stops of varying durations (1 to 10 minutes) at half-the-depth of the
dive – Half Depth Deep Stops (HDDS) – with or without shallow stops at 5 meters (1 to 10 minutes), in the typical
recreational diving range of 18 to 40 MSW.
The introduction of a HDDS generally reduced post-dive precordial Doppler detectable venous gas emboli (PPDDVGE),
with an overall decrease of High Bubble Grades compared to the same dives without HDDS.
The data suggest that the inclusion of a HDDS on dives between 25 and 30 MSW, with Bottom Times of 25 minutes or
less (i.e., the typical dive profiles performed by recreational divers) reduces decompression stress as measured by
PPDDVGE.
The value of HDDS in reducing PPDDVGE was not as evident for shallower (18 – 21 MSW) and deeper dives (40 MSW),
when brought to the limit of the respective No-D bottom time according to USN Dive Tables, and showed conflicting
results.
Further investigation is now being planned to unravel the apparent ambiguity of HDDS at these depths.
PL 3
An Overview of Diving and Diving Medicine in China
Tao Hengyi, Sun Xuejun, Liu Wenwu, Sun Qiang, Mao Ding
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
This article presents a brief history of diving and diving medicine in China, especially the developmental track of diving
and diving medicine, as well as symbolic achievements in this field since the founding of New China. It also provides an
overview of research on submarine escape or rescue in the Chinese Navy.
1. A brief history of diving and diving medicine in China
Diving is an important means of human fighting against and adapting to Nature. But there is no textual research about
when and where diving was started and who pioneered it. According to Shang Shu (the Book of History) published in the
st
21 century BC in China, there were already people who used pearls and beads from seabed as tributes to Yu Empire in
st
ancient China, indicating that the Chinese people started their diving activities as early as in the 21 century BC.
Nevertheless, the primitive purpose of human diving was for survival rather than for material contributions. During nearly
50 years from 1900 when China began using modern diving apparatus (Hookah diving apparatus and manual pumps) to
the eve the founding of new China, there were only a few diving units with fewer than 100 divers and shabby equipment,
much less diving safety operation regulations, and there were few people who had professional knowledge of diving
medicine, let alone diving medical supervision systems. The maximum diving depth was about 20 meters. Diving
accidents happened frequently, and the incidence of diving disease was high. Once divers were diseased, nobody helped
them. Therefore, many divers were handicapped by lifetime diving illnesses, and the case-fatality rate remained at a high
level.
After the founding of new China, under the attendance and care of the Chinese Communist Party and the people’s
government the diving cause in New China has a new lease of life and develops flourishingly. Diving facilities are being
built up in succession. Right after the founding of the Chinese Navy, arescue-salvation force was set up, which has been
expanded and reinforced increasingly. The Ministry of Communications established marine rescue & salvage bureaus in
Shanghai, Guangzhou and Yantai. The Ministry of Petroleum and the Ministry of Railways also established specific diving
units. The number of professional divers is on the increase and their diving skills are becoming more sophisticated. Diving
technology has now been widely used in economic constructions (such as tapping and exploitation of seabed resources),
military activities (such as submarine rescue and underwater reconnaissance), scientific research and sports. Diving
medicine has been developing out of nothing and expanding increasingly. In 1954 the Institute of Naval Medicine was
inaugurated in China, which includes a research department specialized in diving medicine. In 1960, the Faculty of Naval
Medicine was extended in Shanghai Second Military Medical University, which is the only institution of higher learning that
can train diving physicians in China. Today diving medicine in China is essentially appropriate to the development of
diving technology and the demand of divers’ safety and health. To facilitate academic exchange in diving and diving
medicine, in 1980 Shanghai took the lead and established the first Hyperbaric Medicine Society in China. In 1982 the
Chinese Navigation Society also established the Diving Rescue & Salvage Committee. As you know, Chinese Society on
Hyperbaric Medicine was established in 1992.
Before the Cultural Revolution, China carried on a close-door policy and did not know about the world, and the world knew
even less about China. Before 1987 when Professor LI Wen-ren and NI Guo-tang, two senior masters in diving
th
and hyperbaric medicine in China, first attended the 9 International Congress on Hyperbaric Medicine, not a few
international scholars in this field even had no idea about whether China had diving operation deeper than 30 meters. So,
when Professor Ni presented his MSc thesis at the meeting, a delegate even raised such a question, “Is there diving
operation deeper than 30 meters in China?” It is DENG Xiao-ping’s open-to-the-outside-world policy that enables the
world to know about China and enables China to know about the world. Today, I’d like to take this opportunity to let the
world know more about China, especially about China’s diving and diving medicine.
2. The developmental track of diving and diving medicine in New China
The first article about diving decompression sickness (DCS) was officially published in 1955. In 1960 China began using
hyperbaric equipment for clinical hyperbaric oxygen treatment. The research and development of the surface
decompression table and the underwater stage decompression table of air diving were started in 1961 and ended with
success in 1965. They were put to practical use by the Chinese Navy in 1977. Civil diving operations in China also follow
these tables. The use of the Navy decompression tables plays an extremely important role in reducing the incidence of
DCS and in guaranteeing divers’ safety and health.160-msw deep heliox diving training was first started in 1962 in China.
During the construction of Nanjing Changjiang Bridge in 1964, a total of 544 time-person air diving operations were
undertaken to a depth of 66 meters, where the technique of surface decompression using oxygen was used without
causing a single case of DCS, reaching the world advanced level. The first practical use of deep heliox diving was in 1965
in inspecting the quality of bridge piers, reaching the depth of 83 meters. In 1969, the scuba diving equipment was
developed successfully in China. The first experiment of 200-msw heliox simulated diving was undertaken in 1970. In
1973 two divers used air diving into a mud cave equivalent to 92 msw to salvage a lost large drill. Although the attempt
failed because of nitrogen narcosis, this was the greatest depth that air diving has been reached in the world. In 1975
deep heliox diving reached 156 mfw in China. In 1976 the first experiment of human simulated air saturation diving was
undertaken, the saturation depth being 20 msw. In 1977 the first experiment of human simulated nitrox saturation diving
was undertaken, reaching the saturation depth of 20-36.5 msw. During the experiment, air excursion diving successfully
reached 50-70 msw in all 181 time-persons. In 1977 a diver, Mr Long, air dived to 64 meters and worked at the bottom for
25 min. But unfortunately, he was out of control and blew up to surface suddenly. As soon as he reached surface, he was
paralyzed and comatose. To save this severe DCS diver, Mr. MU Ze-ling, a military diving physician accompanied the
patient to the hyperbaric chamber, where the pressure was raised to 11 ATA using compressed air to meet the need of
the patient’s condition. They stayed at the maximum pressure for 60 min, and the total duration of decompression was 85
h and 55 min. The patient was saved successfully. This is the maximum air pressure to which humans have been exposed.
This practice reverses the previous inference that “human exposed to compressed air ≧ 11 ATA is bound to cause
unconsciousness and narcotic coma”. Generally speaking, when the chamber pressure is ≧6-7 ATA, the mixture of heliox
should be inhaled to prevent nitrogen narcosis. But as most hyperbaric chambers in China did not have the condition of
inhaling heliox at that time, inhalation of 11 ATA compressed air was the last straw to save the patient’s life. This is also a
full reflection of how dedicated the Chinese medical workers are in rescuing the wounded and saving the dying. Mr. MU is
not only a military diving physician but also a teacher of diving medicine in the Department of Diving Medicne, Faculty of
Naval Medicne, Second Military Medical University, Shanghai China. Owing to his selflessness and dedication, Mr. MU
was honored as “Model Teacher” in 1978 by the Central Military Committee. In 1979 two experiments of simulated nitrox
saturation diving were undertaken in China: the saturation depth of the first experiment was 36.5 msw for 26 days, during
which air excursion reached 60-75 msw in 76 time-persons, and the saturation depth of the second experiment was 50
msw for 12 days. Both experiments were successful, taking the lead in the world both in nitrox saturation depth and
duration of nitrox saturation exposure. Up to now, no nitrox saturation diving experiment has been reported to exceed the
record of 50 msw and 12-day stay. In the same year, an on-the-spot nitrox saturation diving operation was performed,
reaching 36.5 msw and staying at saturation depth for 6 days, during which air excursion reached 64 msw in 14
time-persons. Marine in situ practices in China have demonstrated that the results of these simulated nitrox saturation
diving experiments are convincing, reliable and practicable. Still, in 1979 the first experiment of simulated helioxsaturation
diving was undertaken, reaching the saturation depth of 120 msw and 2-day stay at saturation depth, during which the
excursion depth reached 165 msw in 3 time-persons. During 1977-1980, a famous salvage project was completed
successfully in salvaging a Japanese sunken ship called “AWAMARU”in a depth of 48-69 msw. The operation used air
diving throughout, involving 13604 time-persons. This has been the largest and longest salvage project in China, in which
only 80 cases of DCS occurred with an overall incidence of 0.59%. In 1980 three Chinese Navy divers succeeded in
locking out from a submarine at a depth of 205 msw during their study in France Navy. In 1980 the first treatment table for
air diving DCS was developed in China and has been proved to be therapeutically good. In 1981 an experiment of
simulated heliox saturation diving was undertaken, during which three divers stayed at a depth of 302 meters for 43 h, and
returned to surface safely and successfully after 12-day decompression. In 1982 the first deep submergence rescue
vehicle (DSRV) designed and manufactured by China with self-dominated intellectual property rights cruised its first
voyage. In 1989 an experiment of simulated heliox saturation diving was undertaken by the Chinese Navy, in which four
divers stayed at the depth of 350 msw for 3 days (72 h and 10 min), and excursion diving reached 370-374 msw in 8
time-persons. After 14-day decompression all four divers returned to surface safely. That was the deepest record of
saturation diving in Asia at that time. In 1997 the Chinese Navy conducted a heliox saturation diving training on the sea,
the storage depth of which was 120 mswn for 48 h, during which the excursion dive reached 149 msw in 8 time-persons.
In 2001 the Chinese Navy conducted two heliox saturation diving trainings. The saturation depth of the first training was
140 msw for 48 h, during which the excursion dive reached 166 msw in 8 time-persons, and that of the second training
was 160 msw for 48 h, during which the excursion dive reached 182 msw in 4 time-persons. It was in 2006 that saturation
diving was first used for commercial purposes. During October to December 2006, Shanghai Salvage Bureau undertook a
commercial heliox saturation diving operation for replacing oil pipes at a depth of 96 msw in South China Sea; the
maximum depth of excursion diving was 105 msw. During August to November 2007, Shanghai Salvage Bureau
undertook a 115-msw commercial heliox saturation diving operation in the South China Sea Oil Field for clearing,
measuring and installing oil pipes; the maximum depth of excursion diving was 124 msw. In April 2008, the same
company undertook a 135-msw commercial heliox saturation diving operation for the same tasks as the previous ones;
the maximum depth of excursion diving was 153 msw. In these commercial operations, PPO2 at the saturation depth was
maintained at 0.4-0.5 ATA; PPCO2 was maintained at <0.0055 ATA; chamber temperature was 28.1-30.5℃; and relative
humidity was 56.6-81.1%. During the process of decompression, PPO2 in the chamber was raised to 0.6 ATA. The whole
compression was divided into two phases: at first, the pressure was raised to 5 msw using 20/80 He-O2, and the second,
the pressure was continued to compress to saturation depth using 2/98 He-O2 at a rate of 1-2 m/min.The saturation
decompression rate was controlled at 40-60 min/m. During these commercial saturation diving operations, except two
cases of skin infection, no other medical problems occurred because of excellent diving medical supervision.
3. Submarine Escape in China
Submarines are among the most important combat weapons for the navy and specially favored by the navy because of
their good stealth and strong assaulting ability. But like aeroplanes that may be threatened by air crashes and automobiles
that are likely to be involved in road accidents, submarines have their own problems. In exercises or combat operations,
submarines may run on collisions and rocks, or have mechanical problems, or lose the floating ability when they are
attacked by the enemy. It is roughly estimated that from 1900 to 2001 a total of 434 submarine accidents happened during
the peace-time training exercises in American, Russian, British and French armed forces, excluding those that are not
reported because of confidential reasons. These accidents involved 176 sunken submarines at least killing 2900 crew
members. The most astonishing accident was Russian Kursk submarine due to torpedo explosion on August 12, 2000.
The submarine sank to the 108 msw sea floor killing 118 soldiers and officers on the ship. To preserve living strength,
timely and effective rescue measures must be taken to help the survivors escape from the wrecks. Therefore, how to help
crews escape from the wrecks has long been the focus of attention for navies of all countries. The earliest study on
submarine escape techniques and methods in the world was in 1851. As the submarine force in China was established at
a relatively late time, the study on submarine escape techniques and methods was started in 1960, but has been
progressing quickly. In 1970, type 2-8 submarine escape equipment and the decompression table for submarine escape
were developed successfully, by which individual escape in the depth of 120 msw can be effected. Individual escape
follewed by mass escape would provide escape from a 200 msw depth. Subsequently, studies on fast ascent and its
medical supervision were started. During 1976-1979, simulated 10-310 msw animal experiments were conducted, and in
1980 experiments were performed on humans. In 1983, the human simulated 100 msw fast ascent experiment was
accomplished successfully. In 1987, the submarine escape training from the depth of 52 msw through the torpedo tube
was accomplished. This is an important creation of the Chinese Navy, because fast ascent from submarine in other
countries is implemented through a specific one man escape chamber (OMEC) on the submarine. It has been confirmed
by practice that submarine escape from torpedo tube is feasible. In 2002, the study of simulated 153 msw fast ascent was
successfully accomplished and created the deepest record of fast ascent for submariners in Asia, thus upgrading China’s
research in this field to the world advanced level.
PL4
DCS Incidence of Commercial Dive Works In Japan and Evaluation of the Profile on Multi-Level Diving By
Integration of Nitrogen Accumulation in Each Body Tissue
1
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1
1
1
Yoshihiro Mano , Masaharu Shibayama , Masahisa Komiya , Shin-ichiro Togawa , Nobuo Yamami , Kazuyoshi
1
2
Yagishita , and Mahito Kawashima
1. Hyperbaric Center, Tokyo Medical & Dental University Hospital, Japan
2. Kawashima Orthopedics Hospital, Japan
Commercial dive work has been strictly controled in Japan, so there were few accident cases recently. According to our
data which we have consulted since 1995, the DCS incidence was 0.095 % ( 31 bends per 32,639 dive ) in air dive within
60 m depth and 0.099 % ( 3 bends per 3,038 dive ) in heliox dive works from 30 to 90 m depth. In saturation diving work
by heliox within 200 m depth, it has been 0.38 % ( 2 bends in 529 dive ) since 1983. We have used different dive
methods like as scuba, air line, bell and saturation. And also deffernt decompression tables like as the Japanese or U.S.
Navy standard, surface, and/or our original tables included saturation, however it depends on the dive conditions. This
result has been evaluated as considerably better than other commercial dive companies in Japan.
However in recrational or fishermen divers, there have been recognized near 40 to 50 diving accidences and 20 to 30
divers have died in each year. And some of them have died by the cause of DCS or AGE. Their diving profiles and the
continued decompression methods are obviously risky or self-seeking. So we have developed a new analyzer system to
evaluate dive profile through integration of the nitrogen gas accumulation in each body tissue from 5 to 240 min half
saturation time. We can calculate the accumulated tisue gas tension during diving activity in each 5 second and can
judge whether the diving profile has the risk of DCS or not at the surface by means of compareing it with Workman’s M
Value.
Japanese standard regulation has now been looking again and it will bocome to better and safer working field in near
future.
PL5
Traumatic Spinal Cord Injuries – is There a Role for HBO?
1
2
Jochen Freier , MD; Armin Kemmer , MD
1. Consultant für Diving and Hyperbaric Medicine (EDTC), Anaesthesiology and Intensive Care, Vice President of the
German Society für Hyperbaric Medicine (GTUEM), Tagesklinik Hofheim, Germany
2. Consultant für Diving and Hyperbaric Medicine (EDTC), Anaesthesiology and Intensive Care, Habach, Germany
Spinal cord injuries are still among the conditions, which are extremely difficult to treat.
Damage of the nerve strands embedded in the lumen of the spinal cord often results in permanent paraplegia. In addition
to direct damage to the spinal cord through ruptures and crushes, the injured tissue suffers from a lack of oxygen in the
aftermath of an accident. This causes the cascading release of various tissue hormones like cytokines and prostaglandins,
which lead to the swelling of tissue, local inflammation and, ultimately, cell death. These processes cause additional
damage to the site of the injury and often result in irreversible damage. The basic principle of surgical therapy for thoracic
and lumbar spinal cord injuries relies on decompression, reposition and the subsequent stabilization of the unstable
segment. In addition, early administration of methylprednisolone, ideally already at the site of the accident, is
recommended.
These treatments ultimately aim at improving the tissue’s blood supply and restoring aerobic cell metabolism as quickly as
possible. This is the rationale for hyperbaric oxygen therapy. Analogous to the treatment of crush trauma, HBO results in a
rapid reoxygenation of tissue, which helps to maintain viability and can thus reduce the loss of function.
CNS injuries will cause local changes within a few minutes. These changes not only occur in neurons but also in glia cells.
In contrast to CNS neurons, peripheral neurons are able to cover a greater distance downstream of the site of the injury,
and there, to form synapses to transfer excitation signals to their target cells. By contrast, CNS neurons stop the process
of regeneration immediately once they have reached the site of the injury. The rapidly forming gliatic scar prevents
continuing axonal growth (Müller et al. 2005, Stichel et al. 1998). The inhibition of cellular collagen synthesis leads to the
permeability of the lesion scar for regrowing axons (Müller et al. 2005).
In a study by Murakami (2001), the neurological functions of rabbits with spinal cord ischemia improved when HBO
therapy was initiated after 30 minutes. However, no improvement was seen when therapy was started after 6 h. Huang
(2003) also observed an improved neurological recovery from spinal trauma in rats when HBO therapy was given.
Likewise, Gamache (1980) could demonstrate a more rapid recovery of motor scores in spinal cord trauma after early
HBO therapy. In the Trauma Center Murnau, 35 out of 40 patients with acute traumatic paraplegia showed a significant
improvement under hyperbaric oxygen therapy. Asamoto et al (2000) could also achieve a significant improvement under
hyperbaric conditions. Likewise, Ishihara et al. (1997) observed a positive effect for the application of hyperbaric oxygen
therapy.
In the Trauma Center Murnau a randomised study design was developed, which is presented herein.
The positive effect could be confirmed in several trials. If this effect is solely due to the swell-reducing effect and the
improved oxygenation has not been adequately studied. The application of hyperbaric oxygenation after ordinary injuries
has shown that its application results in reduced scar formation, i.e. collagen production, which could be another reason
for the positive effect of hyperbaric oxygen therapy in spinal cord injuries.
Further studies on the possible changes in collagen production under hyperbaric conditions would be desirable.
PL6
Hyperbaric Oxygen Therapy in Acute Traumatic Peripheral Ischemic Diseases
Wang Gang M.D. Ph.D. Emergency Dept & Hyperbaric Oxygen Dept.
First Affiliated Hospital, China Medical University, Shenyang, China
HBO can be used as adjunctive therapy for trauma patients with crush injury, compartment syndrome, and other acute
traumatic peripheral ischemia (ATPI). The rationale for HBOT is the need to counter the effects of tissue hypoxia arising
as a direct consequence of vascular injury and as a secondary consequence of trauma and infection.
Physiological and Pharmacological Basis of HBO Therapy for ATPI
1.Hyperoxygenation. 2.Increasing in oxygen diffusion distances. 3.Promoting cellular proliferation, accelerating collagen
deposition. 4.Stimulating capillary budding and arborization. 5. Modulating nitric oxide production. 6.Possibly preventing
iNOS up-regulation. 7.Enhancing oxygen radical scavengers, reducing neutrophil endothelial adherence, thereby
decreasing I/R injury. 8.Vasoconstriction and reduction of edema. 9.Maintenance of cellular function. 10. Accelerating
microbial oxidative killing. 11.Improving select antibiotic exchange across menbrances. 12. Interfering with bacterial
propagation.
Crush injury/ Skeletal muscle-compartment syndromes
Crush injury and skeletal muscle-compartment syndrome (SMCS) are two related conditions that arise as a consequence
of trauma. Common features include ischemia and hypoxia at the injury site, a gradient of injury, and the potential for self
perpetuation of the injury.
1. Etiology
2. Pathophysiology
3. Ischemia
4. Edema
5. Gradient of injury
The principles of treatment for crush injury
Surgical and orthopedic interventions for crush injury are based on the type of injury and should be independent of the
decision whether or not to use HBO. That is, HBO should not be used as an excuse to delay surgery or not do surgery.
However, when the decision to use HBO is made, it should be started as soon after the injury as possible. If there are
delays in starting surgery, And HBO is available, it should be given while awaiting the availability of the operating room.
Scheme of Adjunctive HBO Therapy
There is no single ideal protocol for using HBO in crush injury. Treatment schedules are largely a function of clinical
judgment and experience. The maintenance of tissue oxygenation is critical in the immediate post injury period.
The management of Skeletal-Muscle Compartment Syndrome
The diagnosis of compartment syndrome is based on the mnemonic of “five Ps. The monitoring of the pressure in
compartment is important for evaluation of the condition and progression of injured tissue.
The principle of therapy for SMCS
Management of the established SMCS is fasciotomy. This decompresses the muscle and immediately lowers the
compartment pressure, thereby restoring perfusion to the contents of the compartment.
Convincing laboratory studies and clinical researches show statistically significant reduction, in loss of muscle function,
metabolites associated with muscle injury, edema and muscle necrosis with HBO.
Scheme of Adjunctive HBO Therapy
HBOT in Flap Reconstruction and Skin Grafts
Normal skin grafts and flaps with adequate blood supply do not requir HBO. HBO therapy is extremely useful where the
skin grafts or flaps suffer from compromised microcirculation or hypoxia. The benefits of HBO on flaps arise from a
systemic elevation in oxygen tension rather than a local effect. In addition, HBO therapy prevents neutrophil adherence
and subsequent vasoconstriction following ischemia.
Compromised Flaps
Too frequently, a compromised flap is allowed to progress over the days following surgery until visible signs of necrosis
obviate the use of HBO; delayed treatment with HBO cannot revive dead tissue. The resulting disappointment, as well as
the associated patient dissatisfaction, can be avoided by rapid diagnosis of the flap problem and early involvement of the
hyperbaric physician.
Diagnosis of Flap Compromise
Compromised tissues are usually hypoxic with oxygen tension of less than 30mmHg. With flaps, the etiology of the flap
ischemia and compromise may result from technical causes including improper flap design, closure with tension, pedicle
or tissue damage, hematoma, or prolonged operative ischemia. Non-technical causes include arterial vasospasm, flap
edema, post-operative infection, and patient deterioration. Special flap problems include low arterial inflow, total arterial
occlusion, partial venous congestion, and total venous occlusion.
HBO Treatment for Compromised flaps
The use of HBO therapy is appropriate only when:
1. The flap problem has been defined
2. There is documented perfusion of flap
3. Appropriate surgical salvage measures have been considered first
4. HBO therapy can be performed in an expedient manner
Scheme of HBO therapy for compromised flaps
HBO therapy for compromised flaps should be based on the type of flap, classification of ischemia, and effects of
treatment of HBO, which decided HBO therapy regimen.
HBO Therapy for I/R injury
Initially, the focus in acute ischemia caused by trauma should be restoration of blood supply. Replantation should be
completed as soon as possible with repair of disrupted arteries and fasciotomies if necessary. Both clinical experience
and the previously summarized research experience advocate for HBO treatment in I/R injury and results are positive and
often dramatic in our experience. If muscle ischemia time is greater than four to six hours there is a significant risk of
severe I/R injury, muscle necrosis, and loss of the affected extremity. Skin is more resistant to I/R injury but it is likely to
occur after eight hours of ischemia as in the case of a digital amputation. We, therefore, recommend HBO therapy for all
patients with muscle ischemia time greater than four hours and skin ischemia time greater than eight hours.
A retrospective controlled review of compromised free flaps and replanted extremities with greater than six hours of
primary and any secondary ischemia showed 100% salvage rate when HBO therapy was initiated within 24 hours of injury
and 0% salvage rate when HBO therapy was initiated greater than 72 hours after the injury. The major effects of I/R injury
are felt to occur within the first 4-7 hours of reperfusion. Since some irreversible tissue damage occurs after this time it is
important to take patients to receive HBO therapy immediately postoperatively, even if they are still intubated.
Conclusion
Are there evidenced-based criteria to justify the use of HBO in crush injuries and SMCS? When the American Heart
Association criteria are used, HBO meets the criteria for a Category 1 indication. With the ten-point evidenced-based
evaluation systemⅠdevised, crush injuries receive seven points and compartment syndromes five points. A score of five
points or more qualifies the intervention as evidenced-base.
PL7
The Application of Hyperbaric Oxygen Therapy in Critical Disorders
Niu Ko-Chi
Department of Medical Research and Department of Hyperbaric Oxygen, Chi Mei Medical Center, Tainan, Taiwan, China
Hyperbaric oxygen therapy (HBOT) has been widely applied in severe clinical disorders. However, HBOT is suggested to
be hold when body temperature of the patients is higher than 38.5°C and whether HBO may exaggerate oxi dative stress
as results of tissue destruction and subsequent ischemia-induced inflammatory response in critical disorders remained to
be investigated.
MATERIAL AND METHOD: In order to study the effect of HBO on oxidative stress in critical condition, we used the
animal model of Heat stroke (HS) in Sprague-Dawley (DS) rats to mimic multiple organ failure and high body temperature.
Adult male SD anesthetized rats were exposed to an ambient temperature of 43 °C to induce HS. The mome nt in which
mean arterial pressure began to decrease from peak levels was arbitrarily taken as the onset of HS. rats were randomized
to several groups and given a) no resuscitation (NBA, normobaric air) after onset of heatstroke; b) HBO (100% O2 at 2.5
ATA for 1 h, 2 h, or cyclic administration); c) hyperbaric air (HBA; air at 2.5 ATA for 1 h); d) normobaric hyperoxia (NBHO;
100% O2 at 1.0 ATA for 1 h); or e) hyperbaric oxygen 8% (HBO 8%; hyperbaric 8% O2 at 2.5 ATA for 1 h).
RESULTS: Survival time was 19 ±1, 131 ± 18, 72 ± 14, 68 ± 12, or 45 ± 11 min, (n = 10) respectively for NBA, HBO 1h,
HBA, NBHO, or HBO 8% group. The HS-induced arterial hypotension, bradycardia, decreased arterial levels of pH, PaO2,
and SO2%, increased arterial levels of TNF-alpha, and increased values of cellular ischemia and damage markers, and
neuronal damage scores in the cortex were significantly reduced by HBO resuscitation. The cerebrovascular
dysfunctions (evidenced by hypotension, intracranial hypertension, and cerebral hypo-perfusion), hypercoagulable state
(evidenced by increased values of activated partial thromboplastin time, prothrombin time and D-dimer in plasma), and
tissue ischemia/injury (evidenced by increased levels of creatinine, serum urea nitrogen, aspartate aminotransferase,
alanine aminotransferase, and alkaline phosphatase in plasma, and dihydrobenzoic acid, lipid peroxidation, and oxidized
form glutathione/reduced-form glutathione ratio in hypothalamus) that occurred during HS were all suppressed by HBO
therapy immediately after the onset of HS.
CONCLUSION: We successfully demonstrated that HBO and to some extent, HBA was found beneficial in resuscitating
experimental HS. HBO effectively reduced HS-induced arterial hypotension, plasma TNF-alpha overproduction, and
cerebral ischemia and damage and improved survival. The current reuaults indicate that HBO may resuscitate rats that
had a HS by decreasing multiple organ dysfunction and brain oxidative stress. We report the case of a patient who
displayed a classic HS with multiple organ dysfunction and hypercoagulable state resistant to conventional whole body
cooling and antipyretic therapy, necessitating the use of HBOT to rescue from death.
PL8
Hyperbaric Medicine: an European Perspective
Daniel Mathieu
Centre Hyperbare, Hôpital Calmette, Centre hospitalier Régional et Universitaire, Lille, France
Hyperbaric Medicine has a long history in Europe since the first mention of potential therapeutic application of high
ambient pressure for therapeutic purpose goes back to the seventeenth century. Modern application of Hyperbaric
Oxygen (HBO) started in the late 1950's in the Netherlands for anaerobic soft tissues infections and in the United Kingdom
for carbon monoxide poisoning. Since then, a large number of hyperbaric centers have been implemented throughout
Europe, an intense experimental research has been performed and a large clinical work has been done. However, since
the mid 1980's and the appearance of the Evidence Based Medicine, European hyperbaric physicians felt the need to
improve their organisation and the quality of care delivered by HBO centers. This lead to the development of the
"European Committee for Hyperbaric Medicine" (ECHM), which goads are to define commonly accepted indications for
HBO, to issue standards for clinical practice, personnel education, technical procedures and safety. There goals were
achieved through the organisation of Consensus Conferences, Workshops, reports from ad hoc sub-committees. As a
representative body near the European Union, ECHM was successful in promoting an important research program (COST
B14) and the issue of a European Norm for therapeutic hyperbaric chambers (EN 14931).
Nowadays, even if not similar everywhere, a typical European Hyperbaric Centers is hospital based and implanted in or
near an Intensive Care or an Emergency department and is composed by an air-compressed multiplace hyperbaric
system, an hospitalization ward and an out patient clinic. Intensive care patients are commonly treated by HBO and
considerable efforts are done to closely monitor treatment effects through, for example, oxygen pressure measurements
or blood flow at the targeted spot.
PL9
The pretective effect of Hyperbaric oxygen preconditioning on ischemic/hyoxia injury
Xuejun Sun, Hengyi Tao, Weigang Xu, Runping Li
Department of diving medicine, Second Military Medical University. Shanghai, China
Hyperbaric oxygen has been widely used as a primary therapy in patients with carbon monoxide poisoning,
decompression sickness, and arterial gas embolism, and as an adjunctive therapy for the treatment of various diseases
accompanied by impaired oxygen delivery Interestingly, Hyperbaric oxygen has also been tested to produce ischemic
tolerance in stroke models and in organs such as spinal cord, heart and liver, suggesting that Hyperbaric oxygen
produces a wide scale protective effect, and may be a safer preconditioning stimulus as compared with other stimuli such
as hypoxia. Yet the mechanisms underlying its neuroprotective effects remained poorly defined. This paper review the
hyperbaric oxygen preconditioning references and our own some experiment results recently. And try give some
suggestion for further research in this field.
PL 10
Disruption of Viral Pathogenesis Following Patient Exposure to Hyperbaric Environments with Altered Inspired
Gas Mixtures
Stephen D. Guthrie, MD, PhD and Barbara R. Guthrie, MD
Designed Altobaric Research Institute, Livonia, Michigan, USA
Introduction
There is a broad spectrum of susceptibility to, and virulence of, viral infections. Some of this variance is controlled by the
8,13,25,29,46,50
43,44,50
while some is the consequence of the host’s prior life experiences
. This inconsistency
host’s genetics
in severity of clinical response to the same infecting agent has prompted many inquires into the innate capacities of the
3,4,9,24,51
mammalian cell to resist such infections
. Harnessing the power of these inherent anti-viral mechanisms is a
compelling therapeutic stratagem. Further, non-pharmacologic interventions avoid the untoward consequences of all
drug-based regimens, these being, direct toxicity, drug interactions, and the universal aftereffect of inducing resistant viral
mutations.
2,6,8,33
The relative intolerance of the infecting pathogen, compared to the infected host, to marked environmental changes
suggests an attractive tactic. Saturation divers, residents of undersea habitats and orbiting space stations, and the
participants in conventional hyperbaric therapy routinely experience such changes in their ambient milieu.
Environmental manipulations producing direct inhibition of viral pathogenesis, or secondary augmentation of
immuno-competence, would be effective clinical instruments. The Food and Drug Administration’s first approval of a
controlled clinical trial studying HIV/AIDS patients undergoing hyperbaric chamber treatments is a step towards this goal.
Literature Review
Relevant publications are categorized either as clinical descriptions of viral infections purposely treated under hyperbaric
conditions, or as mechanistic postulations as to how such therapeutic benefits could have occurred.
1. Experimental and Clinical Reports:
Using either tissue cultures or experimental animal models, the effects of hyperbaric oxygen exposures on viral
22,38,47
pathogenesis appeared in the literature more than 40 years ago
. A wide variety of viral pathogens have been
11,22,44
examined including DNA viruses, RNA viruses, and ones with or without envelopes
. Not all types of hyperbaric
28
exposures evoked a positive effect , but consistently the encounter produced some effect on viral penetration, replication,
or expression. As early as 1974 experiments were designed to delineate those anti-viral effects caused by the chamber
22,28
pressure versus those induced by the gas composition
.
In 1997 a 3-year compilation of individual HIV/AIDS patients treated with hyperbaric oxygen exposures for symptomatic
indications documented clinical improvements beyond that which could be expected from their concurrent anti-retroviral
41
23
treatments . The current FDA trial on HIV/AIDS patients treated with daily hyperbaric exposures is conducted using air
before refinement of the inhaled gas mixtures is introduced.
Several individual cases have also been published chronicling improvements in patients with non-retroviral infections such
17
20
as viral encephalitis and viral hepatitis . Treating the latter clinical condition evolved into a limited prospective study
30
with positive results . The completion of a prospective, randomized trial in viral hepatitis will further our knowledge of
whether these particular viruses are inhibited by the marked redox imbalance induced by oxygen under high pressures.
2. Mechanistic Proposals:
*
Direct virocidal effects: With the possible exception of the tantalizing prospect that nitrous oxide (NO) might have a
1,18,49
direct virocidal effect
, most of the gases with proven virus-killing capacity would be too toxic for patient
10
administration . It is possible that gases with virostatic potential could trigger the host’s intrinsic mechanisms for defusing
15,44
the lethal viral effects
but that is a speculation without any supportive observations.
*
The “membrane” phenomenon: The observed “membrane” effect of gases under pressure is a general biological
27,48
phenomenon long embraced by hyperbaric medicine
. Such effects can be at the cell’s surface or within any
membrane-encased intracellular compartment. The ion channel effects of gases, particularly inert gases, have been
42
extensively studied in excitable membranes but those same perturbations would occur at all membranes . Particularly
intriguing is the finding that xenon, a very inert noble gas at 1 ATA, actually becomes a general anesthetic agent under
32
hyperbaric conditions . Membrane modeling based on the stereoscopic delineation of anesthetic gas-receptor
8
interaction may help select which gases should be studied under pressure. The latest generation of highly active
36
antiretroviral therapy (HAART) drugs specifically inhibits membrane penetration by the virus . This clearly is another
battleground wherein environmental composition could be a valuable addition to the therapeutic armamentarium.
*
Enhanced immuno-competence: The effects on the immune system of marked alterations in ambient pressure and
12
inhaled gas composition have been known for many years . These particularly have been studied with regard to the
45
reduced immuno-competence observed in long-term occupants of space environments . There are also laboratory
38,47
observations of increased pathogenicity of certain viruses under hyperbaric conditions
. Conversely, given the proper
20,21,25
pressure/gas combination, viral pathogenicity can be diminished and immuno-competence can be strengthened
.
Again, the summary statement here, as with the potential for membrane phenomenon manipulation, is that changes in the
host’s ability to address viral infections can be augmented by the ambient conditions. The search must be for that ideal
environment, with the supposition that the environment with the greatest viral lethality will likely be determined by the
inherent biology of the specific offending virus.
*
Redox balance manipulation:
Another general organism response to hyperbaric environments is imbalance of the redox equilibrium provoked by the
reactive oxygen species generated in profusion under such conditions. There are clinical anecdotes documenting viral
17,20,30
hepatitis patients’ improvement following hyperbaric oxygen therapy
. Several laboratory models directly implicate
14,25,31
the reactive oxygen species as the anti-viral instrument
. These two synchronous lines of investigation have come
6
together as a definitive postulate on disease intervention in the class of enveloped viruses . Further investigation will
reveal if the reactive oxygen strategy can be employed in all viruses, or only certain classes, or at only certain vulnerable
watershed moments in the course of viral infections. As clinicians, the patient trial processes should be started even as
laboratory investigators pursue the intricate mechanisms whereby these beneficial effects occur.
*
iRNA mechanism(s) The mechanism, or more properly the family of mechanisms, that act to derail, mute, or
inactivate the pathologic genetic material introduced by a virus have been formally denominated the “silencing
interference RNA” system. Several acronyms exist (siRNA, iRNA, RNAi) all alluding to this same process. The most
compelling scientific information from a clinical standpoint derives not from bench-top laboratory experiments or patient
29,35
treatment examples but from descriptions of genetic variances which have resulted in individuals
, or whole
13,46,50,51
populations
, having the inherent capacity to “ignore” the fatal viral infusion. In a word, those fortunate cells or
individuals are capable of “silencing” the fatal genetic instructions. Many of these fortunate adaptations have likely
50
29
evolved over millennia but they may appear spontaneously, and quite suddenly . There are vigorous attempts to
harness the siRNA mechanism(s) for immediate clinical utility, and there are sophisticated laboratory tools for quantifying
changes in these processes. It should at least be born in mind that accessing changes in the siRNA system(s) could be
done contemporaneously with the other clinical trials suggested here.
Research Protocol
In April of 2005 the Food and Drug Administration approved the submitted protocol for, and issued permission to proceed
23
with, a Phase-II Clinical Trial on the effects of hyperbaric exposures in patients with HIV/AIDS . That regulatory body
40
thus accepted the results of the Phase-I study on healthy volunteers . Further, that approval signaled the FDA’s
recognition of the scientific premise that such altered environments would positively influence the progress of this disease
based on published laboratory experiments and clinical case reports. The FDA’s assessment of the proposal and protocol
allowed them to assign a “Non-Significant-Risk” (NSR) categorization to this trial. This NSR designation obviates
inclusion in the study of components assessing sensitivities and allergies, drug interactions, and risks associated with
concurrent medical conditions or treatments. The results of this Phase-II study will determine the design and sample size
of the Phase-III trials to determine final efficacy.
Discussion
Any definitive discourse must await the results of the Phase-II Clinical Trial. In the absence of those results any
discussion here ought simply to declare, and reinforce, the reasons why this study is proceeding. Vigorous and colorful
discussions will undoubtedly follow completion of this trial, discussions regarding the meanings, the mechanisms, and the
direction(s) of the next investigational steps. For the moment, the following assertions should be added to the working
body of conversation in this discipline:
1 – Ambient pressure changes and altered inspired gas mixtures have a demonstrable effect on the clinical course of
patients with viral infections, both through effects on the invading pathogen and on the host’s ability to resist that insult.
2 – Both redox imbalances and inert gas membrane effects can produce such effects. The design of the prescribed
gas/pressure blend may depend on the biological characteristics of each individual pathogen.
3 – Such environmental manipulations are spared many of the untoward consequences of drug-based therapeutic
regimens. Further, the potential synergy between hyperbaric treatments and administered medicaments could lessen
the emergence of resistant strains of the virus that follows use of each drug.
It is fortunate that studies on environmental manipulations are not encumbered by the unwelcome physical liabilities linked
to pharmacological interventions. Further, it is fortunate that Hyperbaric Medicine has the talent, the intent, and the clear
mandate to extend the borders of this therapeutic modality.
PL 11
Carbon Monoxide Poisoning Clinical Findings, Common Errors, And Early diagnose
2900 cases
Jordi Desola, MD, PhD, Joan Sala, MD.
Hyperbaric Therapy Unit of Barcelona, Barcelona, Spain
A prospective analysis of
Carbon Monoxide Poisoning (CMP) is one of the best documented indications for HBO, where many research has been
done, and important experience has been cumulated from all the world. In spite of that, the general opinion of the medical
community is not unanimous and it remains confused. Results are really encouraging, but some sceptical attitude about
the role of hyperbaric oxygen (HBO) still remains among some hospitals in the area. Many of these attitudes can be
explained by a lack of knowledge of the toxicology, pathophysiology and clinical pattern of this frequent inhaled
intoxication. The majority of the cases in Catalonia happened within the domestic environment, so the role of primary
assistance teams can be essential. However we have observed a large series of common errors, both in the primary
assistance medical centres as well in the most important general hospitals, which being so repetitive can not be
considered as casual. We have analyzed our series of near to 3000 cases of CMP received in our unit, and we are
pointing out the most frequent and relevant errors.
1.DELAYED DIAGNOSIS. Its condition of tasteless, colourless, and odourless, can maintain Carbon Monoxide (CO)
hidden once the source is extinguished or simply the windows of the room are open. In such condition, erroneous
diagnoses are frequently established in urgency departments and primary assistance cabinets, like Encephalitis, Stroke,
Epilepsy, Dysbaric disorder, Tensional Cephalalgia, Occupational conflict, Collective Hysteria, Food poisoning, or
Peripheral Vertigo.
2.CONFUSION ASPHYXIA/ POISSONING. CMP is very often a hidden disaster. Only in catastrophic situations, or in
cases where coal gas is obviously present, the existence of CO in the environment is presumed. Liquated gases from
petroleum (propane, butane, and so on) do not contain CO so they are not toxic. However in case of free escape the
inhalation of propane or butane can produce serious asphyxia, but not a poisoning. If the combustion of these gases is
incomplete, CO can be formed. In such cases signs and symptoms of asphyxia and CMP will combine and careful
attention is needed to associated disorders like smoke inhalation, or Adult Distress Respiratory Syndrome.
3. ERRONEOUS EVALUATION OF CARBOXYHAEMOGLOBIN LEVEL. Even when CMP is almost certain, some
doctors only accept this diagnosis if the carboxyhaemoglobin (HbCO) percentage is elevated. And even in these cases,
they only consider intoxication as severe if the HbCO elevation is very high. This attitude implies a true ignorance about
both the pathophysiology of this intoxication, as well as the physiology of the haemoglobin oxygen transport and the
carbon monoxide left deviation of the curve. HbCO only indicates the haemoglobin linked to CO, but nothing is known
about the plasmatic CO levels. However, it is well known that in mild cases, patients often have important HbCO
elevations, while other patients in deep coma can have low levels. An HbCO elevation only informs that the patient has
been exposed to CO at some point during the preceding hours, but neither a correlation with severity nor with prognosis
can be established. It is frequently forgotten that the worst toxic role of CO depends on the direct toxic effect over
kytochrome-oxydase mitochondrial breathing.
4.ERRONEOUS EVALUATION OF GASOMETRICS AND PULSE-OXIMETRY. Patients poisoned by CO often have
nearly normal gasometrical values. A mild hypoxemia may be sometimes observed even in seriously ill patients. Gas
blood analytical machines only detect pH, haemoglobin, and arterial pressure of Oxygen and Carbon Dioxide. The other
parameters like Bicarbonates, Base Excess, or Oxygen Saturation are calculated -not measured- so when an important
quantity of haemoglobin is blocked by CO, the results shown by conventional machines are erroneous. Only Cooxymeters
can determine the real oxygen content measuring the actual Oxygen Saturation, but these apparatus are not always
available in small hospitals, and almost never in primary assistance medical centres. Pulse-oximetres are really a kind of
photocolourimetres and they give erroneous results in case of CMP as well. The recently appeared Pulse-cooximetres
can be the most valuable aid in the early diagnose of CMP especially on site for rescuers, first responders, or primary
assistance specialists.
5. IMPROPE ESTIMATION OF SIGNS AND SYMPTOMS. Signs and symptoms of CMP are very unspecific. Cephalalgia,
Nausea without vomiting, Bradipsyquia, Asthenia, and transitory Consciousness Disturbance are the most common in the
not extremely serious cases, which are the most frequent. Some books of internal medicine and on toxicology, explain
that one of the most typical symptoms of CMP is the Sherry skin rush, while in our series it has been found in only the
20.3% of the cases. On the contrary, some recent papers consider the Sherry skin rush as a very rare finding. It is obvious
that the majority of the patients are not aware of this sign thus they do not report it, and it can remain hidden if it is not
actively searched in the emergency department.
6. INNECESSARY CORRECTION OF METABOLIC ACIDOSIS. These patients are usually in real acidotic condition,
which may be more related to the severity of the intoxication than the HbCO level. First responders, and urgency
physicians in hospitals sometimes try to "correct" this acidosis, and their patients become alkalotic after having received
unnecessary doses of bicarbonates. Metabolic acidosis is mainly related to the left deviation of the haemoglobin curve,
and it can become a kind of defence against hypoxia trying to achieve a more stable oxygen transference. A moderate
acidosis is a positive event and need not to be a concern. Only extreme acidosis in the most severe cases, which can be
by itself a cause of major damage, need to be corrected.
7.NON JUSTIFIED ADMINISTRATION OF HYDROXICOBALAMIN. Cyanide (CNH) can be formed in cases of fire in
closed environment, very high temperature, and combustion of synthetic and plastic materials. Following the so called
“Baud’s criteria” a combined CO plus CNH poisoning can be assumed in some cases of patients rescued from fires. Some
experimental laboratory data, not clinically controlled, suggest that very high doses (between 2-5 grams endovenously) of
Hydroxicobalamin (HC) can be the antidote of CNH. This overestimated measure has become very popular in some areas
and first responders have incorporated HC among their emergency procedures. However HC has no action over the
mitochondrial breathing disturbance since only blocks the inhaled CNH, but the patient will persist hypoxic if high quantity
of oxygen is not given. Furthermore, HC has no action over CO, so there is no reason to apply in pure cases of CMP in
which no criteria of CNH poisoning are estimated, and on the other hand HC is very expensive and it can delay the
application of the only etiological treatment with HBO.
8.OVERESTIMATION OF THE UTILITY OF NORMOBARIC OXYGEN. Many patients start to improve from the moment
of the rescue from the intoxication site. In fact, they frequently arrive at the hospital in nearly normal status. Applying
normobaric oxygen may result in an apparent improvement in the less serious cases, but this amelioration does not
correspond to a real healing. High quantities of normobaric oxygen can moderately increase the oxygen concentration in
the blood, but the oxygen deliver to the cell will be always done under atmospheric diffusion pressure. Theoretically, the
half life of HbCO breathing normobaric oxygen is 80 minutes. But in practice some patients have received normobaric
oxygen for several hours in primary hospitals previously to be sent to our unit, and the mean value of HbCO in the moment
of the arrival to the emergency room of our hospital is still high.
9.DELAY OR NEGLIGENCE IN APPLYING HYPERBARIC OXYGENATION. The majority of the guidelines or protocols
in Emergency department of general hospitals are still conditioning the indication of HBO for CMP patients to HbCO
values over 20% or even over 40% what implies a lack of knowledge of the real pathophysiology of CMP and even of the
toxicology of CO. Thus physicians from emergency rooms wait for the result of determination of HbCO before deciding
whether or not the patient must be sent to the Centre of Hyperbaric Medicine. The delay in our area since the moment that
patients arrive to the hospital until they arrive to our unit is near to 7 hours. And many seriously poisoned patients are not
sent to Hyperbaric medical centres simply because that at this time the HbCO is already low, in spite of the fact that the
patient has suffered a serious poisoning.
10. IGNORANCE OF THE LATE NEUROLOGICAL SYNDROME. Many general practitioners and hospital doctors are
unaware of the simple existence of this serious neurological demyelinization syndrome that appears after some days of
fully symptomatic remission. Not only practitioners but some hospital neurologists are equally uninformed. Several well
known and internationally accepted textbooks on internal medicine, and even some books on toxicology do not mention
the risk of late neurological symptom in case of CMP or it is described as a neurological sequella of the acute intoxication..
This was not an exhaustive list of common errors committed by both practitioners from primary assistance as well as
doctors from emergency departments in General hospitals. Many of them are based in a lack of information of some
aspects that are well described in basic textbooks of respiratory physiology. Some other errors depend on a sceptic
attitude proning to accept any drug treatment but at the same time very reluctant against not pharmacologically supported
treatments. Data is nowadays relevant. Research is deep and wide. But there are no more serious blinds than those not
wishing to see.
投稿发言
Free Paper Abstract
OR01
HBO and cytostatic chenotherapy
Christtian Heiden
German Professional Soc. Hyperb. Chambers
Objective: There is a certain amount of reservation for
applying HBO to patients who have a history of malignant
tumor treated with cytostatic drugs. As hypoxic tumor cells
do not react well to treatment wether it is radiotherapy or
cytostatic drug there is a ratio for applying HBO as an
adjuvant for cytostatics. Method: A survey of the literature
on this topic is given in detail, also concerning the different
classes of cytostatics. Own experience of the last 5 years
is presented. Results: Few publications deal with HBO
and cytostatics. There are encouraging papers published.
The number of cases treated with HBO after cytostatic
tumor therapy and even so as an adjuvant to cytostatics is
very limited and far beyond evidence based medicine. In
all our own cases the HBO treatment did not cause any
problems. Conclusion: The application of HBO after
cytostatic treatment is feasable in general. Precautions
have to be made concerning special substances. As the
experience published is very small the adjunctive
application of HBO should be limited to very special cases
after all treatment options have failed and the patient
would be lost otherwise. Informed consent of the patient
for the treatment is indispensable.
OR02
Hyperbaric Oxygen: Does it Promote Cancer
Recurrence or Metastasis? An Update
John Feldmeier
University of Toledo Medical Center; Radiation Oncology
Department; Toledo Radiation Oncology, USA
Objective: To review and update the literature related to
cancer growth, recurrence and metastases after
hyperbaric oxygen. Methods A comprehensive review of
this topic was published by Feldmeier et al in 2003. Since
that time several new publications have been added to the
body of literature on this topic. The prior review as well
and the more recently published literature, found by
computer based literature search (Medline), are reviewed.
Results: The literature on this topic can be divided into 1)
Case reports and case series; 2) Randomized trials
designed to investigate hyperbaric oxygen as a
radiosensitizer; and 3) Pre-clinical studies designed to
address the issue in question and encompassing a broad
range of malignancies. Only a rare anecdotal case or
small case series reports cancer recurrence or
progression after hyperbaric oxygen. Much larger case
reports fail to confirm a higher rate of cancer recurrence.
The randomized trials from the era of hyperbaric oxygen
radiosensitization do not suggest an increased risk for
metastases or death due to cancer after hyperbaric
oxygen. The vast majority of pre-clinical studies show
either a neutral effect or a slight suppression of malignant
growth. Conclusions: The preponderance of available
evidence fails to demonstrate an enhanced rate of cancer
growth, recurrence or metastasis. Hyperbaric oxygen can
be given to cancer patients without fear that the treatment
will promote cancer recurrence or progression.
OR03
Hyperbaric Oxygen for Complications of Oncological
Treatment of Paediatric Patients
1
2
2
Michal Hajek , Pavlina Cahova , Jaroslav Sterba , Jiri
3
4
Ruzicka , Pavel Zonca
1. Centre of Hyperbaric Medicine, Municipal Hospital of
Ostrava, Czech Republic, 2. Clinic of Paediatric Oncology,
Faculty Hospital of Brno, Czech Republic, 3. Faculty
Hospital, Charles University of Pilsen, Czech Republic, 4.
Centre for Visceral and Mini-invasive Surgery,
Krankenhaus Wesseling, Köln am Rhein, Germany
Introduction: Use of Hyperbaric Oxygen Treatment
(HBO) for children requires special knowledges for
indication and treatment. Especially nursing of critically ill
patients demands specific equipment inside of hyperbaric
chamber. There is a strong need for close cooperation
between paediatric and hyperbaric specialists for
ensurance of adequate care of sick paediatric patients.
HBO for sick oncological patients is very exhausting for
nursing staff from psychological, organizing as well as
treatment point of view. There is a long tradition of
treatment of children within our treatment centre with
multiplace hyperbaric chamber equipped with perfect
monitoring, ventilation and technical devices, as our
hyperbaric center started to work in 1965 and from that
time more than 16.000 patients were treated here.
Methods and Results:
This is retrospective
observational study. From 1994-2008 total of 33 children
(19 of male sex, 14 of female sex) have been treated for
complications of oncological treatment in our hyperbaric
unit. In the early period the hepatotoxic influence of
cytostatic drugs has been the main reason for HBO
treatment (17 patients). Recently hyperbaric oxygen is
used due to severe posthypoxic encephalopathy(3
patients), after extensive intracranial surgery for malignant
diseases(9 patients) with different grade of brain damage
and neurological deficit, complications due to treatment
of immunity suppressive drugs (sepsis, critical illness
polyneuromyopathy- 3 patients). One children has been
treated for radiation injury of urine bladder. Mean age of
children were 8 years (1-17 years), 22 patients were
treated for blood malignancy, 11 patients for solid tumour.
Mean number of HBO sessions were 7,5 (1-20),
treatment pressure of 1.9 to 2.5 ATA. Among of them,
positive effect has been achieved in 89 to 100 % of cases
due to clinical situation, total positive treatment effect was
described in 31 from 33 patients(94 %).One patient out of
33 (3%) could not finish his treatment due to ear
equillibration problems, in two patients bleeding from
tracheostomy as well as nose occured, but without need
of interruption of HBO sessions, one patient (3%) failed to
achieve positive tendency from treatment. Discussion:
This is a pilot study for creating of basal imagine of HBO
effects in those clinical situations. It is very important to
emphasize that it is a small group of patients, evaluation is
provided retrospectively and without use of any standard
measurement method and parameters. Most of patients
can be evaluated as criticallly ill patients and the complex
treatment has been supplied. HBO treatment for those
patients is high risk due to infectious diseases, immune
suppression therapy, need for isolation of patients inside
of hyperbaric facility. We agree with the presence of
parents during HBO treatment. ENT examination with
paracenthesis, or bilateral tympanostomy with ventilation
tubes insertion within general anesthesia are provided
before of HBO treatment. For assessment of HBO
treatment there have been used standard neurological
examination, electromyography(EMG), or creatin kinase
levels in blood. Conclusion: Objective evaluation of
mechanisms of HBO effects and confirmation of its
positive acting will demand next studies, moreover with
randomised prospective design. Present results show that
HBO can be useful method in treatment of paediatric
oncological patients with neurological deficit and other
oncological treatment complications.
OR04
Hyperbaric Oxygen may accelerate tumour-bearing
mice to death
Qingle LIU, Chenggang Zheng, Xiaohua Hang
Changhai Hospital, the Second Military Medical University,
Shanghai, China
Objective: To explore the influence of hyperbaric
oxygenation (HBO) on life-span in tumour-bearing
mice. Method: Male Balb/c mice were randomly divided
into celiac-tumour group: inoculating tumour cell into
abdominal cavity of the animal, celiac-tumour-HBO group:
inoculating tumour cell into abdominal cavity and then
exposing to hyperbaric oxygen, back-tumour group:
inoculating tumour cell under skin of the back of the
animal, back-tumour-HBO group: inoculating tumour cell
under skin of the back of the animal and then exposing to
hyperbaric oxygen. There are 7 mice in each group. After
s-180 tumour cell was translated to mice HBO was given
once a day in concerned group. The life-span and skin
ulcer were observed. Results: The life-span is 27.57 士
4.4693 days in celiac-tumour-group, 24 士 2.9439 days in
celiac-tumour-HBO group, 63.43 士 21.844 days in
back-tumour group, and 35.14 士 8.934 days in
back-tumour-HBO group. The life-spans in celiac-tumour
group and in celiac-tumour-HBO group are different, but P
value is 0.133204; the life-spans in back-tumour group
and in back-tumour-HBO group are significant and P
value, 0.009002. Conclusion: The life-span of mice in
back-tumour-HBO group was shorten by exposing HBO,
and life-span in celiac-tumour-HBO group is tendency to
be shorten by HBO exposure. The ulcer formation is
postponed by HBO in back-tumour-HBO group.
OR05
Hyperbaric oxygenation (HBO) in mediastinitis treated
with an open mediastinum after cardiac surgery
1
1
Jacek
Kot , Zdzislaw
Sicko , Lech
2
2
Anisimowicz , Wojciech Pawliszak
1. National Center for Hyperbaric Medicine, Medical
University, Gdansk, Poland
2. Department of Cardiac Surgery, Collegium Medicum,
Nicolaus Copernicus University, Bydgoszcz, Poland
Objective: Postsurgical complications after sternotomy
include mediastinal dehiscence and mediastinal wound
infection, either superficial or deep. Mediastinitis, as a
deep wound infection, is a rare complication after the
surgical sternotomy, but it can have high mortality.
Several publications show the positive effect of usage of
the hyperbaric oxygenation (HBO) in mediastinitis with
shorter hospital stay, less morbidity, and cost saving.
Methods: In the National Center of Hyperbaric Medicine
in Gdynia, Poland, between 2005 and 2008 we treated
with HBO thirteen patients (7 males and 6 females) aged
between 53 to 74 years (mean 60.5±6.9 [SD] years) with
an open mediastinum due to mediastinitis in
post-operative period after medial sternotomy for cardiac
surgery. In one case there were clinical and
microbiological evidences of clostridial mediastinitis.
Patients were admitted into the hyperbaric center after
initial surgical debridement of wounds and removal of
sternal wires. Standard care included: daily change of
dressing with wound irrigation and debridement, when
necessary; narrow spectrum antibiotics; HBO sessions
every 12 hours until resolution of infection; adjunctive
treatment as indicated by the clinical status of patients.
Results: Median length of stay in the hyperbaric center
was 12 days (range 9-38), median number of HBO
sessions was 20 (range: 16-54). Median value of
C-reactive protein (CRP) titer at admission was 79.8 mg/dl
(range: 12.8-260.8) and significantly decreased down to
12.8 mg/dl (range: 7.8-103.1) in all patients (p=0.005;
Wilcoxon test). Median value of WBC count at admission
was 7470/ml (range: 5100-18670) and it did not change
significantly at discharge (7420/ml; range: 2870-10890;
p=0.12; Wilcoxon test). In all cases there was
improvement in the local and general clinical status and in
microbiological results, even if not always there was a
bacterial eradication. No major complication occurred
during the HBO sessions. Conclusion: Usage of HBO
should be considered in mediastinitis as a complication of
the cardiac surgery.
OR06
Normobaric and hyperbaric oxygen therapy for
migraine and cluster headache
1
2
Michael
Bennett , Christopher
French , Alexander
3
4
3
Schnabel , Jason Wasiak , Peter Kranke
1. Dept. Diving and Hyperbaric Medicine, Prince of Wales
Hospital, Australia
2. Dept. Neurology, Prince of Wales Hospital, Australia
3. Dept. Anaesthesiology, University of Wuerzburg,
Wuerzburg, Germany
4. Victorian Adult Burns Service, The Alfred Hospital,
Melbourne, Australia
Background: Migraine and cluster headaches are severe
and disabling. Migraine affects up to 18% of women
and cluster headaches 0.2% of the population. A number
of therapies are available, including both hyperbaric
oxygen (HBOT) and normobaric oxygen (NBOT). We
assessed the safety and effectiveness of both for treating
Odstraněno: ¶
Odstraněno: HBO in Tokyo
Medical and Dental
University in 2007 and The
Effects of HBO on Soft
Tissue Injury in Sports
Activity¶
Kazuyoshi Yagishita, Nobuo
Yamami, Seiichiro Togawa,
Yoshihiro Mano¶
Hyperbaric Medical Center,
Tokyo Medical and Dental
University, Tokyo, Japan¶
¶
Objective: In our university
hospital, multiplace
chamber including 3 rooms
and the capacity of 16
patients was set up in 2001.
In 2007, 7970 times
hyperbaric oxygen therapy
(HBO) in 958 patients were
performed, which is the
most patients number in one
institute in a year in Japan.
The purposes of this study
were to report the diseases
and the patients performed
with HBO in our hospital last
year. The effects of HBO on
crush injury and
compartment syndrome
were well documented by
many authors, and
randomized controlled
studies revealed the effects
of HBO on reduction of
necrosis and edema. We
now perform HBO
aggressively to soft tissue
injury including
compartment syndrome,
ankle sprain, knee ligament
injury, and muscle strain.
The purposes of this study
were also to investigate the
effect of HBO on soft tissue
injury in sports activity.
Patients and Methods:
7970 times HBO in 958
patients were performed in
our hospital in 2007. The
number of the patients and
HBO times were evaluated.
In 180 patients with soft
tissue injury in sports activity,
visual analog scale (VAS)
scores and recovery time to
previous sports activity were
evaluated. Results: The
... [27]
Odstraněno: ¶
and preventing migraine and cluster headaches. Methods:
We searched the following in May 2008: CENTRAL,
MEDLINE, EMBASE, CINAHL, DORCTIHM and
reference lists. Relevant journals were hand searched
and researchers contacted. We accepted any randomised
trials
comparing
HBOT
or
NBOT
with any
alternative. Three reviewers independently evaluated
study quality and extracted data. Results: Nine trials
involving 201 participants were included. Pooling of data
from three trials suggested HBOT was effective in
relieving migraine headaches compared to sham
therapy (RR 5.97, 95% CI 1.46 to 24.38, P = 0.01). There
was no evidence that HBOT could prevent migraine, or
reduce the incidence of nausea/vomiting or the
requirement for rescue medication. There was a trend to
better outcome in a single trial evaluating HBOT for
cluster (RR 11.38, 95% CI 0.77 to 167.85, P = 0.08).
NBOT was effective in terminating cluster headache
compared to sham in a single small study (RR 7.88, 95%
CI 1.13 to 54.66, P = 0.04), but not superior to ergotamine
administration in another small trial (RR 1.17, 95% CI 0.94
to 1.46, P = 0.16). Seventy-six per cent of patients
responded to NBOT. No serious adverse effects of HBOT
or NBOT were reported. Conclusion: There was
evidence that HBOT was effective for the termination of
migraine in an unselected population, and weak evidence
that NBOT was effective in cluster. Given the cost and
poor availability of HBOT, more research should be done
on patients unresponsive to standard therapy. NBOT is
cheap, safe and easy to apply, so will probably continue to
be used.
manufacturer’s
instruction.
Results:
Intravenous
administration of lipopolysaccharide (2 µg/kg) caused
increased levels of both core temperature and
hypothalamic glutamate, hydroxyl radicals, and
prostaglandin-E2 accompanied by increased plasma
levels of tumor necrosis factor-α, interleukin-1β, and
interleukin-6. Treatment with hyperbaric oxygen (100% at
253 kPa) once a day for consecutive 7 days prior to or 1
hour after injecting lipopolysaccharide significantly
reduced the lipopolysaccharide-induced elevation of core
temperature, circulating tumor necrosis factor-α,
interleukin-1β, and interleukin-6, and hypothalamic
glutamate, hydroxyl radicals and prostaglandin E2. Direct
injection of tumor necrosis factor-α (20 ng), interleukin-1β
(20 ng), or interleukin-6 (10 ng) into the lateral cerebral
ventricle also caused a rise in both core temperature and
hypothalamic glutamate and hydroxyl radicals, which
could be attenuated by treatment with hyperbaric oxygen.
Conclusion: Hyperbaric oxygen can be used as a
prophylactic as well as a therapeutic agent for prevention
or
suppression
of
endotoxin-related
systemic
inflammation and fever in rabbits.
OR07
Hyperbaric oxygen therapy induces antiinflammation
and antipyresis in experimental studies
1
2
1
Lin Mao-Tsun , Gao Chunjin , Niu Ko-Chi
1. Department of Medical Research and Department of
Hyperbaric Oxygen therapy, Chi-Mei Medical Center,
Taiwan, China
2. Department of Hyperbaric Oxygen Therapy, Chaoyang
Hospital Affiliate of Capital University of Medical Sciences,
Beijing, China
Recent data suggest nitrite as a product of the complex
interplay between nitric oxide (NO), molecular oxygen and
superoxide that takes place during hyperbaric oxygen
therapy (HBOT). A rapid reversible decrease in
endothelial NO bioavailability during hyperbaric oxygen
exposure may possibly be due to this auto-oxidation of
NO. Inorganic anions such as nitrite that were previously
thought to be inert end products of endogenous nitric
oxide metabolism are now being viewed as possible
storage pools of NO-like activity. With the reduction of
nitrite back to NO in the circulation indicated as a possible
mechanism for hypoxic vasodilatation, nitrite is being
investigated as an endogenous signaling molecule and
regulator of gene expression. Given its role in blood flow
regulation and hypoxic nitric oxide homeostasis, nitrite
may not only serve as a diagnostic marker but also find a
role as a potential therapeutic agent. Protein nitrotyrosine
has been identified as a marker of formation of another
potent oxidant, peroxynitrite from nitric oxide. Objective:
To examine changes in the nitrite and peroxynitrite
metabolites of NO in the blood of healthy subjects after a
single HBOT session. Method: Fifty six healthy adults
were included in the study. Plasma nitrite was measured
in 22 test subjects and 12 controls before and one hour
after HBOT with either 100% oxygen or compressed air at
2.5 ATA for sixty minutes. Determination of plasma
nitrotyrosine was done separately with 15 test subjects
and 7 controls using the same protocol. Results: Plasma
nitrite and nitrotyrosine levels were not found to be
significantly altered after the single HBOT in subjects who
Objective: To ascertain whether hyperbaric oxygen
therapy inhibits the increase of glutamate, hydroxyl
radicals, and prostaglandin E2 in the hypothalamus and
reduces fever during lipopolysaccharide-induced systemic
inflammation in rabbits. Methods: Adult male New
Zealand white rabbits, weighing between 2.2 and 3.2 kg at
the start of the study, were used. The pyrogen assay was
carried out with unanesthetized animals restrained in
rabbit stocks. The microdialysis probes were
stereotaxically and chronically implanted into the preoptic
anterior hypothalamus of rabbit brain (the essential
thermoregulatory center) for assessment of glutamate,
hydroxyl radicals, and prostaglandin E2 in situ. For
measurement of serum cytokines, 5 ml of blood was
withdrawn from the marginal ear vein of each rabbit. The
amounts of the cytokines tumor necrosis factor-α,
interleukin-1β, and interlueimin-6 in the serum were
determined by using double-antibody sandwich ELISA
(R&D systems, Minneapolis, MN, USA) according to the
OR08
Measurement of plasma nitrotyrosine and nitrite after
a single treatment with hyperbaric oxygen in healthy
human subjects
Renu Agnihotri, Amy Welch, James Stewart, Dheeraj
Bansal
Saba University School of Medicine, Netherland Antilles
received 100% oxygen therapy as compared to
compressed air. There was a non-significant elevation of
plasma nitrites and nitrotyrosine in both groups of HBOT
subjects as compared to controls that received no HBOT.
Conclusion: Increased oxygenation of tissues due to
hyperbaric therapy may therefore be more significantly
related to the production of reactive metabolites of nitric
oxide than the oxygen content of the inhaled air, in short
term HBOT.
OR09
HBO in Tokyo Medical and Dental University in 2007
and The Effects of HBO on Soft Tissue Injury in
Sports Activity
Kazuyoshi Yagishita, Nobuo Yamami, Seiichiro Togawa,
Yoshihiro Mano
Hyperbaric Medical Center, Tokyo Medical and Dental
University, Tokyo, Japan
Objective: In our university hospital, multiplace chamber
including 3 rooms and the capacity of 16 patients was set
up in 2001. In 2007, 7970 times hyperbaric oxygen
therapy (HBO) in 958 patients were performed, which is
the most patients number in one institute in a year in
Japan. The purposes of this study were to report the
diseases and the patients performed with HBO in our
hospital last year. The effects of HBO on crush injury and
compartment syndrome were well documented by many
authors, and randomized controlled studies revealed the
effects of HBO on reduction of necrosis and edema. We
now perform HBO aggressively to soft tissue injury
including compartment syndrome, ankle sprain,
kneeligament injury, and muscle strain. The purposes of
this study were also to investigate the effect of HBO on
soft tissue injury in sports activity. Patients and Methods:
7970 times HBO in 958 patients were performed in our
hospital in 2007. The number of the patients and HBO
times were evaluated. In 180 patients with soft tissue
injury in sports activity, visual analog scale (VAS) scores
and recovery time to previous sports activity were
evaluated. Results: The number of the patients and HBO
times in decompression illness were respectively 374
patients and 764 times, 87 patients and 934 times
insudden deafness, 34 patients and 740 times in
peripheral vascular disorder including diabetes and
arteriosclerosis obliterans, 47 patients and 1046 times in
osteomyelitis, 180 patients and 865 times in soft tissue
injury related sports activity, 90 patients and 1644 times in
myelopathy and radicuropathy, 19 patients and 220 times
in carbon monoxide poisoning, and 18 patients and 259
times in radiation-induced cystitis. In patients with soft
tissue injury in sports activity, VAS scores improved
compared between pre and post 2-hour HBO. In patients
nd
with 2 grade medial collateral ligament injury of the knee
in HBO group, recovery acceleration to previous sport
activity was observed. Conclusion: In HBO procedure in
our university in 2007, no major patient troubles and no
chamber problems were recorded. The effects of HBO on
soft tissue injury were strongly suggested in this study.
OR10
Adjunctive use of hyperbaric oxigenacion (hbo2) in
the managment of cerebral hemorrhage in a pregnant
patient: a case report.
Chávez A., Uribe R., Sánchez EC.
Hospital Angeles Metropolitano, Mexico
Introduction: The purpose of a craniotomy is
decompress through a cranial bone resection. It does not
reverse the primary injury, but reduces secondary
damage caused by cerebral edema after a hemorrhagic
vascular event. The association of HBO is beneficial if it is
performed in the early stage. Material and Methods: We
reported a 33-year-old female patient who has a cerebral
hemorrhage secondary to an arteriovenous malformation.
The case is complicated because the patient has a 25
weeks pregnancy. HBO (1.8 atm abs / 90 min / QD / 20
treatments) was used a adjunctive treatment as an early
neuroprotector to inhibit ischemia reperfusion injury. MRI
was done before and after HBO. Results: The patient
improved clinically and by MRI. There was a marked
improvement of the motor and cognitive function. The
patient was able to maintain the pregnancy that was
resolved by Caesarean section. There was no secondary
effect of HBO to the neonate. Conclusion: This case was
complicated not only by the cerebral hemorrhage of the
mother but also by a 25 week pregnancy. HBO was used
as an early neuroptotector. It inhibits ischemia reperfusion
injury (I / R) when used at an early stage. There was a
marked clinical and MRI improvement of the mother and
there were no secondary effects to the product, specially
retinopathy of the premature. This is the first case of the
used of HBO for cerebral hemorrhage in a pregnant
woman.
OR11
Necropsic findings of syndrome of intratoracic
hyperpressure in a young diver and a decompression
accident after a deep scuba diving in veteran diver
(two particular cases)
1
2
2
Batle J.M. , Poncela J.L. , Jimenez M.A.
1. MEDISUB Hyperbaric Research Institute, Palma de
Mallorca, Spain
2. Legal Medicine Institute, Palma de Mallorca, Spain
Introduction: On the coasts of our islands, (the Balearic
Islands), the cleanliness and the almost constant
temperature of the waters all year round , creates a great
interest and a great number of followers to sub-aquatic
activities either of a professional or recreative character.
The depths of the Mediterranean sea ranges from 50 to
120 metres with the existence of red coral colonies
highly valued in Jewellery shops. The Government
controls the above mentioned professionals limiting the
number of permits or authorizations to only a few and
these boats are usually worked by a Captain and a sailor.
Materials and Methods: 12 year old patient while diving
and making an uncontrolled ascent suffered a
Intrathoracic Overpressure Syndrome and an Arterial
Gas Embolism. The professional divers are in the habit of
diving depths from 80 to ll0 metres using scuba breathing
Odstraněno: OR09 ¶
Hyperbaric Oxygen may
accelerate tumour-bearing
mice to death¶
Qingle LIU, Chenggang Zhe
ng, Xiaohua Hang
Changhai Hospital, the
Second Military Medical
University, Shanghai, China
¶
Objective: To explore the
influence of hyperbaric
oxygenation (HBO) on
life-span in tumour-bearing
mice. Method: Male Balb/c
mice were randomly divided
into celiac-tumour group:
inoculating tumour cell into
abdominal cavity of the
animal, celiac-tumour-HBO
group: inoculating tumour
cell into abdominal cavity
and then exposing to
hyperbaric oxygen,
back-tumour group:
inoculating tumour cell
under skin of the back of the
animal, back-tumour-HBO
group: inoculating tumour
cell under skin of the back of
the animal and then
exposing to hyperbaric
oxygen. There are 7 mice in
each group. After s-180
tumour cell was translated
to mice HBO was given
once a day in concerned
group. The life-span and
skin ulcer were
observed. Results: The
life-span is 27.57 士 4.4693
days in celiac-tumour-group,
24 士 2.9439 days in
celiac-tumour-HBO group,
63.43 士 21.844 days in
back-tumour group, and
35.14 士 8.934 days in
back-tumour-HBO group.
The life-spans in
celiac-tumour group and in
celiac-tumour-HBO group
are different, but P value is
0.133204; the life-spans in
back-tumour group and in
back-tumour-HBO group are
significant and P value,
0.009002. Conclusion: The
life-span of mice in
back-tumour-HBO group
was shorten by exposing
HBO, and life-span in ... [28]
equipment with three bottles of 3 x l8 litres and lighting
equipment of l50w. besides a bag for the gathering of
the coral . They are in the habit of hanging this from the
neck and the pickaxe is hung from the right hand. The
divers make decompression stops from 51 metres and are
ascending gradually, when they are at l2 metres they go
to surface where they have a decompression chamber
installed in their boat, to continue their period of
decompression returning to pressure again of 12
metres and to make the corresponding stops. To make
the step of humid decompression to decompression in
hyperbaric chamber, the surface maximum time is very
limited to a maximum of 5 minutes. The 74 year old
patient who after making his dive to 84 meters of depth
was 20 – 25 minutes on the bottom and his
decompression stops lost conscience inside the
decompression chamber installed on board.
The
materials used are the usual ones of the Medical Legal
Institute and two cameras. The methodology was the
system used in all cases of death, not natural with
technologies and specific enquiries for these cases.
Results: The Signs that appeared in the case of SIHP
were immediate at the arrival to the surface and were a
neurological type. The findings found the levels of the
different organs which have always been described in
communications and literature are evident and in these
cases, though it is necessary to emphasize certain organs
reveal that they have still not been described. Conclusion
and Discussion: It is possible to state that in the cases of
Traumatic Embolism of Air widespread pathology
happens which affects all organs. It is possible to state
that in these cases, the accumulation of gas is in all the
tissues of the organism, but in the moment of shock,
moreover there are organs in charge of managing the
crisis which also suffer the effects of the embolism.
O12
24 cases of neurological decompression illness - 14
months of a single center experience
1
1
2
Jorge Calderón , Cristian Melián , andrés Reccius
1. Underwater Medicine Service, Hospital Ancud
(UMSHA), Ancud, Chile
2. Pontificia Universidad Católica, Chile
Objectives: To review the first 14 months of operation of
the Underwater Medicine Service of the Hospital de
Ancud, Chile (UMSHA). To describe the cases of treated
neurological decompression illness (NDCI). Methods:
Prospective systematic registry of the conditions with a
detailed profile of diving and neurological examinations
made by a neurologist specialized in diving medicine.
Revision and analysis of these registries. Results: We
review 60 cases of decompression illness treated from
March 2007 to May 2008, 24 (40%) of them had NDCI. Of
these, 13 had involvement of the spinal cord, four of the
brain, and 7 had peripheral nerve disease. All patients
were treated with 6 USN, generally with two prolongations
to 18 meters. Most cases with spinal disease had
paraparesia with a sensitive level and generally without
compromise of propiocepcion. Nine patients didn’t have
anterior spinal cord involvement, one patient had a
complete spinal cord syndrome and one had a syndrome
that couldn’t be systematized. There were 2 cases that
couldn’t be classified in any subtype. Of these cases, four
received lidocaine, and required between 0 and 12
sessions of hyperbaric oxygen therapy (HBO). Of the
patients that did not receive lidocaine, three did not
require more sessions and the rest required between five
and ten sessions. The only patient who had a complete
spinal disease required 25 sessions of HBO. From the
cases that had a cerebral disease, two presented with
alterations in consciousness. One of them died in spite of
hyperbaric treatment and ICU treatment. The other had an
alteration in consciousness and myoclonus and survived
without sequels. The other two cerebral cases had
lacunar syndromes that recovered completely with
hyperbaric treatment. Of the 7 cases of peripheral disease,
6 of them had hypoestesia in the territory of the circumflex
nerve, associated with articular and muscular compromise.
Conclusion: A high percentage of the treated cases of
decompression illness are NDCI. Possibly these cases
are overrepresented. The presence of a “dissociation” or
existence of different patterns of injury in the spinal cord
could be explained because of different physiopathologic
reasons, that is to say, arterial embolization in cases of
spinal disease with respect of the posterior columns,
venous thrombosis in cases of a complete medullary
syndrome, and presence of bubbles in cases of a not
systemizable spinal cord disease. The pattern of complete
spinal disease seems to have a worse prognosis. All
patients had important omissions of decompression.
OR13
Effect of hyperbaric oxygen treatment on traumatic
brain injury rat by magnetic resonance imaging
1
2
Xiang Huang , Chunjing Gao
1. Bejing Shunyi Hospital of China Medical University,
Beijing, China
2. Beijing Chaoyang Hospital of Capital University of
Medical Science, Beijing, China
Objective：
：To investgate dynamic changes of the effect of
hyperbaric oxygen treatment(HBOT) on traumatic brain
injury (TBI) during the initial 7 days by using lateral
fluid-percussion (LFP) rat modal and magnetic resonance
imaging (MRI). Meterials and Metheods ： 30 male
Sprague-Dawley rats were randomly divided into 4 groups:
HBOT group(n=10), TBI group(n=10),1st day TBI
group(n=6), sham control group(n=4). MRI was performed
at 1 day, 3 day, 5 day, 7 day after severe injury on lateral
fluid-percussion. HBOT was performed to rats in HBOT
group each day after 24 hours. The rat brain imaging was
evaluated according to a standard by 2 radiologist who
didn’t know groups divided. All rats were sacrificed
immediately after MRI exam; their brains were removed
and prepared for pathologic exam. Results：There is no
st
difference between HBO and TBI group at 1 day
according to MRI evaluation. The difference tendency
rd
th
present at 3 day and 5 day suggesting better
improvement in HBOT than TBI group. The difference
th
becomes significant at 7 day. Conclusions：HBOT is
effective in accelerating brain tissue repair, promoting the
lesion clearance and hematoma or edema’s absorbing by
dynamic investigation of MRI. HBOT is also protective to
brain tissue after the building of TBI rat modal for 24 hours
which second brain damage have happened, the process
is quantitative change to qualitative change.
OR14
The effect of hyperbaric oxygenation on diabetic foot
ulcers (the HODFU-study). A single centre,
prospective,
randomised,
double-blind
placebo-controlled parallel-group study
1
2
Christer
Hammarlund , Magnus
Londahl , Per
2
3
Katzman , Jan Apelqvist
1. Helsingborg Hospital, Sweden
2. Lund University Hospital, Sweden
3. Malmoe University Hospital, Sweden
Objective: To evaluate effects of hyperbaric oxygenation
(HBO) on long-term ulcer healing in patients with diabetes
mellitus and chronic foot ulcers where further possibilities
for vascular surgery has been ruled out. Methods: 94
Patients from three hospitals in southern Sweden were
enrolled in the study. Randomisation was stratified for
toe-pressure with a cut-off at equal or less than 30 mm Hg.
Both HBO and hyperbaric air (HBA) treatment was given
at the same time in the chamber in a double blind fashion.
The patients received a minimum of 37 and a maximum of
40 treatments at 2.5 ATA and a duration of 90 minutes.
Trancutaneous oxymetri was used for evaluating the
effects of HBO/HBA-treatment. In this presentation are no
subgroups analysed. Results: The median time to ulcer
healing was 5 months in the HBO-treated group and 12
months in the HBA-group. A blinded observer did this
evaluation. Approximately half of the wounds did not heal.
Trancutaneous oxymetri showed successive higher
oxygen levels on the forefoot during the chamber
treatment in the HBO group compared with no rise in
oxygen levels in the HBA group. The differences between
the two treatment groups lasted at least 1½ year.
Conclusion: Hyperbaric oxygenation is a valuable
adjunctive when treating diabetic foot ulcers.
OR15
Hyperbaric Oxygen Therapy for 16 Gas Gangrene
Patients Injured by Earthquake
Pan Fu-Qiong, Zeng Yu, Nie Cai-Xian, Huang Ling-Ling,
Tang Ming-Chan, Li Yu-Feng, Zhang Yun
Sichuan Provincial Hospital, Chengdu, China
Objective: observe the therapeutical effect of Hyperbaric
oxygen for 16 gas gangrene patients injured by
earthquake. Methods: establish emergency isolation
passage, rescue each case with hyperbaric oxygen at the
pressure of 0.25Mpa,7 times per 3 days,20 times totally.
And psychological guidance is added. Results: all
wounds recovered in 16cases,including 4 cases with their
unamputated limbs preserved. Fusiform bacillus can not
be detected. Conclusions Hyperbaric Oxygen can
effectively curb the growth of Fusiform bacillus, decrease
the production of toxin, promote the heal of wounds, lower
the disability rate and raise the survival rate.
OR16
A survey on HBO treatment of traumatic brain injury
this century in China—A important evidence of HBO
improve curative effect and prognosis of Brain injury
Zhou Shurong
First Affiliated Hospital of Nanjing Medical University,
Nanjing, China
This report summarizes 214 articles about HBO treatment
of brain injury published on the proceedings of the 7
national HBO academic congresses since this century.
According to the statistics, 28878 cases of brain injury and
2088 case of PVS have been treated with HBO in this
period. Among the 28878 cases of brain injury, 67.5%～
88.2% were cured or notably effective, the total effective
rate was 89.3%～99.6%. While in the 2088 cases of PVS,
46.7%～58.83% were cured or basically cured, the total
effective rate was 82.25%～97%. In this report 12 articles
were cited, the RCT and Meta-analysis of which were
compared with the data from some brain injure therapy
research centers in the world. The results show that HBO
synthesize therapy is better than the routine treatment in
the improvement of curative effect and prognosis. This
report presente ample evidences for HBO sythesize
therapy in raising effective and improving prognosis.
OR17
Clinical analyses of 429 Cases of Acute CO Poisoning
1
1
2
1
Zhuo Li , Chunjin Gao , Xiang Huang , Huan Ge , Yu Ga
1
o
1. Beijing Chaoyang Hospital, Beijing, China
2. Beijing Shunyi Hospital, Beijing, China
Introduction: Acute CO poisoning frequently occurs in
winter in northern part of China. After CO poisoning,
COHb has an evident increase. It is well known that rising
COHb affects the transportation of oxygen and triggers
the poisoning. Methemoglobin formation results from the
oxidization of the bivalent iron of hemoglobin into trivalent
iron, which lacks the capacity of carrying and releasing
oxygen. However, there is no literature reporting the
change of Methemoglobin formation in acute CO
poisoning patents (ACOP). With the application of Rad-57
Pulse CO-Oximeter, (Masimo Corporation) we have
observed the changes of COHb and MetHb of 429 acute
CO poisoning patients and studied the roles of COHb and
MetHb in triggering acute CO poisoning as well as their
relations. Methods: The subjects are 429 acute CO
poisoning patients in Beijing Chaoyang Hospital and
Beijing Shunyi Hospital from November, 2006 to March,
2007 with 100 healthy people who do not smoke as the
control group. We employed Rad-57 Pulse CO-Oximeter
to observe the levels of COHb and MetHb of the
emergency patients when they went to see the doctor.
After 2h inhaling of oxygen (through nose at the rate of
3L/min) or one hyperbaric oxygen theraphy (2ATA,
breathe oxygen for 60min), the levels of COHb and
MetHb were measured again and the changes observed.
We also recorded general situation, cause of the disease
and clinical classifications etc. of the patients. Then we
utilized SPSS11.0 software for statistic analysis. Results:
CO poisoning of 98% of the patients is due to
inappropriate use of coal stoves. Clinical classification:
Light poisoning 274 cases; intermediate poisoning: 77
cases; severe poisoning: 78 cases. A total of 98
emergency patients have MetHb>1.2% at the first
measurement. The concentrations of both COHb and
MetHb of the patients are evidently higher than that of the
control group (p0.05) although fifty patients out of 58
cases showed small decrease in MetHb. Discussion and
conclusions: (1) Inappropriate use of coal stove is the
main cause of acute CO poisoning in northern part of
China. (2) MetHb may be involved in the
physiopathological process of hypoxia of ACOP patients.
MetHb of such patients is clearly higher than that of the
control group, but only 98 patients have MetHb >1.2%.
This indicates that the rise of MetHb level is not the key
factor leading to hypoxia in ACOP patients. The increase
of COHb level is the main cause. (3) Whether hyperbaric
oxygen
treatment
can
effectively
cure
methemoglobinemia still requires further study. (4) The
Rad-57 Pulse CO-Oximeter provides a noninvasive tool to
greatly facilitate the clinical diagnosis & treatment for CO
poisoning patients.
Table 1
group
COHb (Mean%)
MetHb (Mean%)
Control group
1.28±0.68
0.25±0.12
ACOP group
18.28±8.40
0.86±0.82
P -value
<0.01
<0.01
OR18
Review of Current situation on acute carbon
monoxide poisoning in china
Shuyi Pan, Xiaowen Pan, Yu Zhang, Yan Lu, Xiangen Me
ng, Chen Yang, Mingxin Li, Qi Zhang, Liang Zhang
Navy General Hospital, Beijing, China
To summarize the study on acute carbon monoxide
poisoning (ACMP) of Chinese Units in recent 10 years,
including pathogenesy, clinic treatment and animal model
building. And analyze the existing problems and reasons
of ACMP. The conclusion that morbility foundation of
ACMP and delayed encephalopathy may be poisoning
cascade reactions in which multiple pathomechanisms
play roles; It maybe one of important factor that patient
breathed in concentration of CO for prognosis. The key to
resolve this problem is early intervention. Reviving the
respiration and circulation early to ensure utility filling into
tissues and organs, sustaining cell normal metabolism,
especially to brain tissue. The treatments are including,
active HBO treatment, keeping breathing smooth,
ensuring utility oxygenation and circulation, improving
microcirculation, anticoagulation, reducing plasma
fibronectin. In addition, cytoprotection measures such as
anti-free radicle should be given at the right moment. At
the same time, multicentre unite research should be
carried out for better therapeutic measures.
OR19
Effect of HBO on endogenous neural stem cells in rat
models of acute CO poisoning
Wenlan Wang, Jinsheng Li, Xiaoping Xie
Department of hyperbaric oxygen treatment center,
School of Aerospace Medicine, Fourth Military Medical
University, Xi’ an, China
Objective: To discuss the effect of HBO on endogenous
neural stem cells (NSCs) in rat models of accute CO
poisoning, and to analyses the mechanism of HBO
treating brain injury after accurate CO poisoning.
Methods: After set up models of accurate CO poisoning,
H-E staining was used to observe the pathological
changes of brain tissues and immunohistochemical
staining was used to observe the expression of nestin
(NSCs specified marker) and GFAP (astrocyte specified
marker). Results: The H-E staining showed that the
morphology of neurons in control group were normal, and
which of poisoned groups were degenerated and necrotic
in spots and sheets, and the cortex of CO poisoned
groups were looser as well as the cone cells of
hippocampus were thinner, but in HBO group the
degenerated and necrotic neurons became less. The
immunohistochemical staining showed the following:
expression of nestin and GFAP was normal in quantity
and morphology in control group, in CO poisoned groups
the expression of nestin was increased lightly and the
expression of GFAP was abnormal in quantity and
morphology, in HBO group GFAP positive cells became
normal, but the expression of nestin was increased
obviously. There are nestin/GFAP double positive cells in
cortex where the damage of neurons was extremely
heavy. Conclusion: Compared to control group, the
+
quantity of nestin cells was increased lightly, astrocytes
were proliferated and deformed and neurons get
degenerated and necrotic in poisoned groups and HBO
could make astrocytes become normal and have NSCs
generate, immigrate and differentiate. It is suggested that
the process of brain injury can make NSCs active and
HBO can treat the brain injury by generation, immigration
and differentiation of NSCs.
OR20
Hyperbaric oxygen treatment attenuated the decrease
in regional glucose metabolism of rats subjected to
focal cerebral ischemia: a high resolution positron
emission tomography study
1
2
3
1
M. LOU , H. ZHANG , J. WANG , S. Q. WEN , Z. Q.
4
5
6
1
TANG , Y. Z. CHEN , W. Q YAN , M. P. DING
1. Department of Neurology, the Second Affiliated
Hospital, Zhejiang University, School of Medicine,
Hangzhou, China
2. Department of Nuclear Medicine, the Second Affiliated
Hospital, Zhejiang University, School of Medicine,
Hangzhou, China
3. Zhejiang-California International Nanosystems Institute,
Hangzhou, China
4. Department of Hyperbaric Oxygen, the Second
Affiliated Hospital, Zhejiang University, School of
Medicine, Hangzhou, China
5. Department of Neurobiology, Zhejiang University,
School of Medicine, Hangzhou, China
6. Clinical Research Center, the Second Affiliated
Hospital, Zhejiang University, School of Medicine,
Hangzhou, China
Cerebral hypoxia may be the main component of cell
damage caused by ischemia. Previous studies
demonstrated a neuroprotective effect of early hyperbaric
oxygen (HBO) treatment in various animal models of focal
cerebral ischemia. Neuropathologic study showed that
exposure of HBO may prevent cell death in ischemic
cortex. In the present study, we aimed to assess cellular
function of ischemic rat brain after HBO treatment by
means of a high-resolution positron emission tomography
scanner (microPET) used specifically for small animal
imaging. The male Sprague– Dawley rats were subjected
to permanent middle cerebral artery occlusion (MCAO),
with the regional cerebral blood flow monitored in vivo by
laser Doppler flowmetry. One hour after ischemia, HBO
therapy (3 atm absolute, 1 h) was initiated. Local cerebral
glucose utilization in the ischemic area was measured
before, 1 h and 3 h after ischemia, with
2-[18F]-fluoro-2-deoxy-d-glucose (FDG) as a tracer.
Neurological deficits and infarct volumes were assessed
at 24 h after ischemia. Our study showed that early HBO
therapy significantly reduced infarct volume of brain 24 h
after ischemia. Moreover, glucose utilization in the
ischemic area underwent asevere decrease during 1–3 h
after MCAO, while the early HBO treatment significantly
attenuated the decrease in cerebral metabolic rate of
glucose in the ischemic core of the cortex compared with
controls. We report for the first time the application of
microPET to quantify the rates of glucose metabolism in
the ischemic core of rats exposed to HBO. Our results
suggest that the early exposure of HBO can partially
reverse the downward trend for glucose utilization in the
ischemic core, which might contribute to the reported
beneficial effects of early HBO therapy on permanent
cerebral ischemia.
OR21
Treatment of micro-found drilling cranium and
hyperbaric oxygen on 94 cases of patients with
extradural hematoma
Jincheng CHENG, Yiqun WANG, Bensong YU, Ganf SU,
Shiquan YANG, Tian XIA
123th Hospital of People’s Liberation Army
Objective: To evaluate the curative effects of micro-found
drilling cranium and hyperbaric oxygen (HBO) for patients
with extradural hematoma. Methods: According to the
volume of hematoma and GCS points of patients, ninety
four cases of extradural hematoma were divided into four
groups: conservative group (14cases, the volume of
hematoma<50ml), micro-found drilling cranium I group
(16cases, the volume of hematoma<50ml), micro-found
drilling cranium II group (28cases, the volume of
hematoma≥50ml), micro-found drilling cranium+ HBO
group (36cases, the volume of hematoma≥50ml); their
curative effects, complications, the hospitalization time
and hospitalization cost were compared respectively.
Before and after the HBO treatment, the haemodynamics
parameters of HBO group were detected and compared.
Results: The 94cases got an excellent prognosis. The
sequela, hospitalization time and cost in micro-found
drilling cranium I group were lower than in conservative
group (P<0.05); and in micro-found drilling cranium+ HBO
group were lower than in micro-found drilling cranium II
group (P<0.05). After the HBO treatment, the
haemodynamics parameters of patients were ameliorated,
and the difference were significant when compared with
the before (P<0.05). Conclusion: The treatment of the
micro-found drilling cranium combined with the HBO on
patients with extradural hematoma can significantly
increase the cure rate, decrease mutilation rate, and
effectively shorten the hospitalization time, save treatment
cost.
OR22
The effect of hyperbaric oxygen on the osteoporosis
rats
Wang Peisong, Wan Jin’e, Yang Xizhong, Wang Shuzhen,
Liu Xueling, Lu Qiuning
Department of hyperbaric oxygenation, The affiliated
Hospital of Qingdao University Medical College, Qingdao,
China
Objective: To inverstigate the impact of hyperbaric
oxygen on bone mineral density and bone biomechanical
property . Methods: Reproduce the osteoporosis model
of ovariectomized rats and received hyperbaric
oxygen and lycopene
therapy.
After
12
weeks
therapy,bone mineral density and bone biomechanical
property were measured. Results: The bone mineral
density and biomechanical property of ovariectomized
rats decreased significantly (P<0.05), and it increased
after 12 weeks therapy using hyperbaric oxygen and
lycopene, and there were no significantly between
hyperbaric oxygen and sham groups. Conclusions:
Hyperbaric oxygen and lycopene could improve and
increase the bone mineral density and bone
biomechanical
property
in
ovariectomized
rats;
Hyperbaric
oxygen
and
lycopene have the nearby effect .
OR23
Research on the distribution of oxygen concentration
in the Sinlge Hyperbaric Oxygen Chamber
1
1
1
Qibiao Weng , Hongjuan Wang , Lingzhen Chen ,
1
2
3
4
XuelinChen , Zhenxin Huang , Huai Huang , Xiangxue Li ,
4
4
Guirong Lin , FengxianWei
1.Department of Hyperbaric oxygen, Zhujiang Hospital,
the Second Affiliated Hospital of Southern medical
university, Guangdong province, China.
Naformátováno: Písmo:
(výchozí) Arial, 9 b., Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., Tučné
Naformátováno: Zarovnat
do bloku
Odstraněno: Trial of
cabin-oxygen-concentrati
on distribution of
Odstraněno: single oxygen
pressurized module
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Zarovnat
do bloku, Řádkování:
jednoduché
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Písmo:
(výchozí) Arial, 9 b., není
Tučné
Naformátováno: Zarovnat
do bloku
2.Department of Hyperbaric oxygen, the Second Affiliated
Hospital of Guangzhou medical college, Guangdong
province, China.
3.Department of Hyperbaric oxygen, Guangzhou General
Hospital of Guangzhou Military Area Command of
Chinese PLA, Guangdong province, China.
4.Department of Hyperbaric oxygen, the Hospital of
Guangdong Water Resources and Hydropower
engineering Bureau 2, Guangzhou, China.
Objective ： This study was designed to explore the
distribution of oxygen concentration in different parts of
the hyperbaric oxygen chamber and the possible
influence of the different locations of oxygen inlet/outlet
and the methods of washing cabin. The oxygen inlet/outlet
were located in different ways and the oxygen samples
were gathered in the upper, middle and lower parts of the
chamber in our test. The aim of our study was to select
the most appropriate location of oxygen inlet/outlet and
the most appropriate method to wash cabin so as to
enhance the uniformity and concentration in the chamber,
which could improve the theraputical efficiency of
hyperbaric oxygen. Materials and methods：
：We used 7
choices of combination of different ways letting in or
letting out the oxygen. Meanwhile, whether patient was in
chamber and if the continuous washing was executed had
been concerned. Finally, 15 methods came out under
manifold of situation. Result and Discussion:Three
essential elements: the ways of oxygen inlet/outlet, the
state of cabin, the ways of washing, was aimed in our trial
for each of them takes great changes to the elevation of
oxygen concentration and distributive uniformity. Three
positions of inlet/outlet, three states and three opinions of
washing cabin was developed in raising, stable and
decreasing pressure period with 9 sampling spots set on
the chamber. The trial was executed with the pressure
under 0.1MPa, lasting for 80min and strictly following the
rule of Chinese medical association-HBO branch.
Measurement range is 0-100% with sampling interval of
2min. 1.Influential factors on the distribution of
oxygen concentration. The main factors can be the size
of stretcher and the location of oxygen inlet/outlet.
Unsymmetrical distribution was found, causing by the gas
shot circuit on the bottom of wide size stretcher, which
was widely used in single hyperbaric oxygen chamber. In
contrarily, the oxygen inlet on the top or middle side
braces the concentration to 65-75% with the symmetrical
distribution. On the other hand, the modification on
stretcher make help to the gas diffusion in the chamber.
2.The best method of washing cabin. Only
wash-procedure with the pressure-raise to 0.1MPa
showed distinctive difference of 2% elevation at the top
and 4% at the middle and bottom parts. Moreover, the
normal pressure method can be the best for it raise the
concentration effectively, especially up to 80%in 5
minutes in the middle part. 3.selection of sampling
spots Since the different design of stretcher and the
inlet/outlet locations vary, the regular way to set the spots
on the top of stretcher and middle of chamber fails reflect
the distribution of concentration. However, changing the
inlet position and taking the appropriate way washing
cabin can provide consistent samples everywhere in the
chamber. 4.the distribution of oxygen concentration
We found that the concentrations were same everywhere
in the cabin while the pressure was at 0.1MPa lasting for
40min. Nevertheless, great difference took place while
pressure reducing; concentration on bottom was higher
than that in other parts. The concentration came to
coordinate again when pressure dropped to 0.03MPa.
Suggestion1.The location of sampling spots should be
standardized.2.The location of oxygen inlet/outlet should
be standardized. Oxygen inlet should be set at the top of
the posterior part of the chamber, while oxygen outlet
should be set at the bottom of the anterior part of the
chamber.3.The way to wash cabin should be applied
according to the need of the patient so as to achieve the
appropriate oxygen concentration.4.The production of the
stretcher should be standardized. First, it must be
equipped with a mattress. Second, enough space should
be vacated beside the stretcher in the chamber.5. A new
function should be developed in order to mix the gas
automatically and make help to the gas diffusion in the
hyperbaric oxygen chamber.
OR24
Clinical Research on Effect of HBO plus Electric
Stimulation for Treatment the Cerebral Resuscitation
Peidong Wang, Chunping Wu, Hulong Ma, Kangmin Xie,
Yaojun Zhang, Xia Wang, Aiping Wang
Chinese HBO Medicine Association Research Center of
Brain Recovery, Nanjing, China
To improve therapeutic effect on cerebral brain
resuscitation, a comprehensive therapy with hyperbaric
oxygen (HBO) treatment as the main method was
adopted from 1991 April to 2006 Dec. Among total 973
cases of PVS patients; 14 cases patients were put to use
electrical stimulation (ES) demonstrated more excellent
effect. Judgment of therapeutic efficacy: - Rely on PVS
scoring scale and criteria of therapeutic efficacy (Nanjing,
2001). Basic recovery (consciousness or cognition
recovery) 371cases (38.13%); Obvious improvement
(score ≥ 8 –12 points) 203 cases (20.86%); Obvious
improvement (score ≥ 3 points) 190cases (19.5%); No
improvement (no change, worsening or death) 209 cases;
The general improvement rate is 78.48%; The HBO plus
ES is superior to the results of similar HBO treatment.
Some causes that affect efficacy in treatment of PVS are
analyzed as follows: From the result, it can be seen that
the efficacy of HBO treatment in traumatic cases is
obviously higher than that of non—traumatic cases; the
younger the onset happens, the higher the curative rate is;
the earlier HBO treatment is used, the better the efficacy
is; and HBO plus ES, if available in some indication cases,
shows that the more excellent effect. We suggest that
PVS patients should receive HBO therapy (and certain
cases plus ES) as early as possible is the best choice
OR25
Odstraněno: Huang
1
2
Zhenxin , Weng Qibiao ,
2
Wang Hongjun , Chen
2
2
Lingling , Chen Xuelin ,
3
4
Huang Huai , Li Xiangxue ,
4
4
Lin Guirong , Wei Fengxian ¶
1. Zhujiang Hospital
attached to Southern
Medical University,
Department of Hyperbaric
oxygen, Guangzhou, China¶
2. The Second Hospital
attached to Guangzhou
Medical College,
Department of Hyperbaric
oxygen, Guangzhou, China¶
3. General Hospital of
Guangzhou military district,
Department of Hyperbaric
oxygen, Guangzhou, China¶
4. Department of Hyperbaric
oxygen, Hospital of Second
Water and Electricity Office
of Guangdong Province,
Guangzhou, China¶
Naformátováno: Zarovnat
do bloku
Naformátováno: Písmo:
Tučné
Odstraněno: The medical
oxygen pressurized cabin
which is convenient and
suitable for patients with
different diseases and ages
has been widely used for
decades. Meanwhile, it is
accepted by a majority of
hospitals. To select
appropriate input/output of
oxygen, structure and
washing method is
beneficial to oxygen
concentration elevation and
distribution in cabin,
because pure oxygen is
used as medium for
compression. Three ways of
oxygen input/output(on the
top, middle and bottom of
the cabin), three states of
the cabin(with a bed, with a
bed and a patient, without a
bed and a patient) and three
ways of washing
method(continued washing
method with boosting
pressure, washing method
of boosting pressure of...the
[29]
Naformátováno: Angličtina
(USA)
Effect of Hyperbaric Oxygen Treatment on the content
of VEGF, HIF-1,CX43 in random skin flaps of rabbits
1
1
1
1
Xuehua Liu , Chunjin Gao , Huan Ge , Fujia Liu , Yang
2
2
Liu , Hongying Zhao
1. Department of Hyperbaric Oxygen, Chaoyang Hospital,
Capital Medical University
2. Department of pathology, Chaoyang Hospital,
Capital Medical University
Purpose: To observe the changes of capillary density,
vascular
endothelial
growth
factor(VEGF),
hypoxia-inducible
factor-1(HIF-1),
Connexin
43(CX43)before and after hyperbaric oxygen treatment in
the transplanted random skin flaps of rabbits and to
explore the
mechanism of capillary ingrowth of
hyperbaric oxygen treatment on random skin flaps of
rabbits. Materials and Methods:
12 rabbits were
randomly divided into two different groups: control
group(n=6), hyperbaric oxygen group(n=6). Random skin
flaps models were established on the back of rabbits. The
control group was placed in normal pressure atmosphere
without hyperbaric oxygen treatment. The hyperbaric
oxygen group started to accept hyperbaric oxygen
treatment(2 ATA, 45min per time) after flap
transplantation immediately, twice a day from
post-operation to the third day, then reduced to one time
per day to the seventh day, seven days altogether. we
observed the survival area of random skin flaps and
detected capillary density and content of VEGF, HIF-1,
CX43 by using HE staining and immunohistochemistry at
rd
th
3 day and 7 day respectively. The SPSS11.5 software
was used for statistical analysis. Results: 1.The survival
area of skin flaps in the hyperbaric oxygen group was
rd
singnificantly higher than that of the control group at 3
th
day and 7 day (p<0.01); 2.The capillary density in the
hyperbaric oxygen group was higher than that of the
rd
control group at 3 day (p<0.05),the capillary density in
hyperbaric oxygen group was singnificantly higher than
th
that of the control group at 7 day (p<0.01); 3.The content
of VEGF in the hyperbaric oxygen group was higher than
rd
th
that of the control group at 3 day and 7 day (p<0.05); 4.
The content of HIF-1 in the hyperbaric oxygen group was
rd
th
lower than that of the control group at 3 day and 7 day
(p<0.05); 5.The content of CX43 in the hyperbaric oxygen
group was singnificantly higher than that of the control
rd
group at 3 day(p<0.01),the content of CX43 in the
hyperbaric oxygen group was higher than that of the
th
control group at 7
day (p<0.05). Conclusions:
Hyperbaric oxygen treatment can accelerate capillary
ingrowth by increasing the expression of VEGF and CX43,
so improve the survival rate of transplanted random skin
flaps. Hyperbaric oxygen treatment can improve the
hypoxia state of transplanted random skin flaps and
decrease the expression of HIF-1 in transplanted random
skin flaps.
1,
2
2
3
Wang Youbin , Gao Chunjin , Liu Fujia , Sun Xuejun , Liu
2
Xuehua (Qi Zheng and Gao Chunjin contribute equally in
this experiment)
1. Plastic Surgery Department of Peking Union Medical
College Hospital, Beijing, China.
2. Hyperbaric oxygenic Deperpartment of Beijing
Chaoyang Hospital associated with Capital Medical
University, Peking, China.
3. Department of Chinese Military Medical University,
Shanghai, Chnia.
Objective: To study the interfering effect of
preconditioning hyperbaric oxygenation on skin flap
ischaemia tolerance. Methods: 18 SD rats were divided
the control group and the HBO preconditioning group. In
control group, an extended epigastric adipocutaneous flap
was raised, based on the right superficial epigastric artery
and vein. 3-hours flap ischemia was induced by clamping
the pedicle vessels with microvascular clamp. At the end
of ischemia induction, the clamp was removed and the
flap was sutured back. Rats in HBO preconditioning group
were treated with HBO two days before operation. Flap
surgery began 1 hour after the last HBO treatment. The
operation was the same as the control group. The size of
the designed flap and the survived flap on the fifth
postoperative day was duplicated with transparent paper.
The condition of the flap was recorded with digital camera.
,
The picture of the paper was gotten with scanner and it s
area which was equal to the area of the flap was
calculated with Acrobat soft ware. Data were analyzed
with SPSS soft ware. Results: The average designed
2
flap areas were 51.59 and 52.71 cm in the control group
and the HBO preconditioning group. The average survival
2
flap areas were 7.38 and 15.82 cm . There were obvious
difference between the control and the HBO
preconditioning group (t=4.14, P=0.003) in average
survival area. Conclusion: HBO preconditioning can rise
flap ischaemia tolerance and enhance flap survival.
OR27
Effect of early hyperbaric oxygen treatment on
prevention of disorder of coagulation-fibrinolysis
systems in rabbits with steroid-induced avascular
necrosis of the femoral head
1
2
3
WANG Yong , GAO ChunJin , PANG BaoSen , YANG
4
1
2
JinCai , LI HaiDong , WU LianHua
1. Department of Hyperbaric oxygen, Fuxing Hospital,
Capital Medical University, Beijing, China
2. Department of Hyperbaric oxygen, Beijing Chaoyang
Hospital, Capital Medical University, Beijing, China
3. Department of Respiratory Diseases, Beijing Chaoyang
Hospital, Capital Medical University, Beijing, China
4. Department of Orthopaedics, Beijing Chaoyang
Hospital, Capital Medical University, Beijing, China
Objective: To establish animal model of steroid-induced
OR26
Hyperbaric Oxygenation Preconditioning Induce Skin
Flap Ischaemia Tolerance in Rat Model
avascular osteonecrosis of the femoral head (SANFH)；
To investigate the effects of hyperbaric oxygen (HBO)
therapy on histopathology, coagulation and fibrinolysis of
SANFH. Methods: 1. Experimental animal and group:
Male adult New Zealand white rabbits, ranging in weight
from 2.5 to 3.5 kg, were obtained from experimental
center of capital university of medical sciences and
randomly divided into eleven groups including Group N
(control group); Group M24h, M48h, M1, M2, M4, M6(model
groups); GroupH1, H2, H4, H6(HBO groups). Each group
had eight rabbits. 2. Model establishment and HBO
therapy: According to Yamamoto T et al’s method,
animals in group M and H reveived lipopolysaccharide
(LPS) 20µg/kg intravenously 2 times at an interval of 24
hours,
injected
methylprednisolone
20mg/kg
intramuscularly into the gluteus medius 3 times at
intervals of 24 hours after the second injection of LPS.
Animals in Group H1, H2, H4, H6 were received HBO
therapy after the second injection of LPS, 2.0 ATA, 1 hour
daily, once a day. Group H1, H2, H4 , H6 were treated 1,
2 ,4 weeks and 5 weeks respectively. 3. Experiment
parameters:
Antithrombin-III
(AT-III)
、
Thrombomodulin(TM) 、 Tissue-type
plasminogen
activator(tPA)
and
Plasminogen
activator
inhibitor-1(PAI-1 ) were measured in each group; Animals
in group M and H were killed at 2 weeks, 4 weeks and 6
weeks respectively after the second injection of LPS and
the tissue samples of the femoral head were observed
with light microscopic examination.
Results: 1.
Histopathologic examination: In group N, the bone
trabeculaes align trimly in subchondral zone of femoral
head. Empty lacunae were found occasionally. Large
hematopoietic tissues were seen in marrow with fat cells
disseminated sporadically. In group M, it was
distinguished that fat cells were increased in both the
number and the size, whereas hematopoietic tissues were
decreased; Subchondral bone and bone trabeculae
showed empty lacunae, pycnotic nuclei and apparently
discrete; Among trabeculae, fat cells showed hyperplasia
and hypertrophy, such pathologic changes showed
particularly significant in group M4, in which marrow were
nearly accumulated with fat cells and the disruption and
fusion of fat cells were seen obviously. In group H, the
number of fat cells was more than that in group N, less
than that in group M, obviously changes were found in
group H4 and H6; Apparently trabeculae loose were not
found; pycnotic nuclei and empty lacunae rate were
significantly decreased than those of group M. Fibrous
tissue becomes more prominent in marrow in group H6
and there were apparently active osteoblasts surrounding
bone trabeculae in metaphyseal area. 2. Hematologic
examination: AT-III : Compare to group N, group M were
significantly higher at 24 hours after the first injection of
LPS(P<0.05), began to decrease at 48 hours, and
reached minimum at 1 week (P<0.05), Group H were not
different in comparison with group N, whereas obviously
decreasing at 4 and 6 weeks(P<0.01); TM: Compare to
group N, group M began to increase at 24 hours after
the first injection of LPS, and reached maximum at 1
week(P<0.01), group H were significantly lower than
group M at 1week(P<0.05), whereas more higher at 4
weeks(P<0.05); tPA: Compare to group N, group M began
to decrease at 24 hours after the first injection of LPS and
reached minmum at 2 weeks(P<0.01), group H were
significantly higher than group M at 2 weeks(P<0.05),
whereas not different than group N; PAI-1: Compare to
group N, group M began to increase at 24 hours after the
first injection of LPS and were significantly higher at 48
hours, 1 and 2weeks(P<0.01), then began to decrease
after 2 weeks, the value were not different than group N at
4 and 6 weeks. group H were significantly lower than
group M at 2 weeks(P<0.01), whereas not different than
group N. Conclusion: HBO intervention can inhibit
hyperplasia and hypertrophy of fat cells in marrow, reduce
the extent of osteonecrosis and enhance the ability of
fibrous reparation and bone reconstruction after
osteonecrosis; HBO therapy can improve obstruction of
coagulation and fibrinolysis of SANFH.
OR28
Hyperbaric oxygen preconditioning protects against
traumatic brain injury at high altitude
Shengli Hu, Rong Hu, Fei Li, Yongzhi Xia, Hui Meng, Gao
yu Cui, Hua Feng
Department of Neurosurgery, Southwest Hospital of the
Third Military Medical University, Chongqing, China
Background Recent studies have demonstrated that
preconditioning with hyperbaric oxygen (HBO) can reduce
ischemic and hemorrhagic brain injury. The aim of this
study was to investigate the effects of HBO
preconditioning on traumatic brain injury (TBI) at high
altitude
and
examine
the
role
of
matrix
metalloproteinase-9 (MMP-9) in such protection. Methods
Thirty-nine male Sprague-Dawley (SD) rats were
randomly divided into three groups: HBO preconditioning
group (HBOP n=13), high altitude group (HA n=13) and
high altitude sham operation group (HASO n=13). All
groups were subjected to head trauma using a weight
drop device except for the HASO group. The rats in the
HBOP group received 5 sessions of HBO preconditioning
(2.5 ATA, 100% oxygen, 1 hour daily) and then were kept
in a hypobaric chamber at a pressure of 0.6 ATA(simulate
the pressure of high altitude of 4000 meters) for 3 days
before operation. The animals in the HA group received
control pretreatment (1 ATA, room air, 1 hour daily), then
followed the same procedures to the HBOP group. In the
HASO group, rats were only opened a skull window
without injuring the brain. Twenty-four hours after TBI,
neurological function and brain water content of 7 rats in
each group were examined and 6 rats in each group were
killed
for
hematoxylin-eosin
staining
and
immunohistochemical
analyses. Results
The
neurological outcome in the HBO group (0.71±0.49) was
better than that in the HA group (1.57±0.53; PP<0.01).
The brain morphology and structure presented by light
microscope was destroyed greatly in the HA group,
including massive cells necrosis, conspicuous edema of
interstitium, hemorrhage, and so on, while less
pathological injuries occurred in the HBO group.
Compared to HA group, pretreatment with HBO
significantly reduced the number of MMP-9-positive cells
(92.25±8.85 vs. 74.42±6.27; P<0.01). Conclusions HBO
preconditioning can attenuate TBI in rats at high altitude.
The decline of MMP-9 expression may contribute to HBO
preconditioning-induced protection of brain tissue against
TBI.
OR29
The effect of hyperbaric oxygen on the proliferation
and death of Nasopharyngeal Carcinoma Cells and its
mechanisms
Zhengrong Peng, Juan Liu, Weihong Zhong, Pingtian Xia
n
Xiangya Hospital, Central South University, Changsha,
China
Objective: To investigate the effect and the possible
influencial mechanisms of hyperbaric oxygen(HBO) on
the proliferation and death in human nasopharyngeal
carcinoma(NPC) cell line CNE2Z by viewing the inhibition
ratio of proliferation(IROP) and mortality rate(MR) and the
content
of
superoxide
dismutase(SOD)
and
malondialdehyde(MDA) of nasopharyngeal carcinoma
cells through HBO disposal. Method: Nasopharyngeal
carcinoma(NPC) cell line CNE2Z were divided into 3
groups randomly, Group A: control group; Group B:
hyperbaric
oxygen(0.20MPa);Group
C:hyperbaric
oxygen(0.25MPa). The IROP in CNE2Z cells of all groups
were detected by MTT reduction assay and the MR were
detected by PI staining; as well as the content of SOD and
MDA were detected. Result: The IROP and MR of B, C
groups were dramatically increased comparing with A
group(P0.05);There were no statistical difference in the
content
of
SOD
between
A,
B
and
C
groups(P>0.05);There were statistical difference in the
content of MDA between A、B and C groups(P0.05).
Conclusion: HBO disposal could increase the IROP and
MR of human NPC cell line CNE2Z, and elevated the
MDA content in nasopharyngeal carcinoma cells, it had
hinted that HBO disposal could inhibit the proliferation of
NPC cells and promoted the death of NPC cells by
increasing the content of MDA.
OR30
Effect of hyperbaric oxygenation (HBO) on unilateral
spatial neglect (USN)
Liu Jinglong
Heilongjiang Rehabilitation Hospital, Ha’erbin, China
Objective: To evaluate the effect of Hyperbaric
Oxygenation (HBO) on unilateral spatial neglect (USN)
and basic activities of daily living (B-ADL) after stroke.
Methods: Sequentially access 93 stroke patients with
hemiplegia admitted to the Heilongjiang Province
Rehabilitation Hospital, according with the following: first
stroke, no aphasia, no dementia, no cognitive disorder
and serious system disease, were classified upon
admission as having USN (group A; n = 43; 46.2% of the
entire sample) or not having such disturbance (group
B(control group ); n = 50; 53.8% of the sample). The age
of all was between 35 and 75 ,and began to get
rehabilitation within 4weeks .By ‘cross-out’ test, ‘digit
cancellation’ test, ‘line-bisection’, clock drawing, ‘free
hand drawing’ test, we examined the unilateral spatial
neglect. When 3 of the 5 tests are abnormal or more, we
thought the patient had unilateral spatial neglect. Both
groups received standard rehabilitation treatment at most
6 weeks after stoke onset, including daily physiotherapy,
occupational therapy, traditional Chinese medicine and
other therapy in accord with individual needs. The group A
was randomly divided into two groups –group
A1(experimental group)(22) and A2 (observation
group)(21).And group A1 was in Hyperbaric Oxygenation
(HBO) synchronously ,but group A2 not .HBO was 2
hours per day and 6 times per week . The Modified
Barthel Index (MBI) was used to assess patients' capacity
in B-ADL. Assessment was done upon admission to
rehabilitation and 6 weeks afterwards. Results: Before
treatment, the patients in A1 and A2 scored significantly
lower with BI than those in B (P < 0. 05). After treatment,
the patients in all groups scored significantly higher with
BI assessment (P < 0. 001). However, the score with BI
assessment in A2 was lower than those in B, but no
significant difference (P >0. 05), and there was no
significant difference between A1 and B (P > 0. 05). But
there was significant difference between A1 and A2 (P< 0.
05). Conclusion: USN had significant impact on the
recovery of the independent living of stroke patients. The
rehabilitative intervention for USN may improve ADL
performance of stroke patients with USN.
壁报交流
Poster Discussion
P01
Postoperative treatments with hyperbaric oxygen and
management of critical complications in 181 patients
with severe brain injury
Xiaowen Pan, Shuyi Pan, Juan Meng, Yu Zhang, Xiangen
Meng, Yan Lu, Huijun Hu, Chen Yang, Qi Zhang, Mingxin
Li
Navy General Hospital, Beijing, China
brain injury is more frequently occurred among vegetative
state patients. A standardized nomenclature and
diagnostic criteria are needed. The pathogenesis
remained unknown and its therapy is only focused on
symptomatic treatment. HBOT won’t be disturbed after the
symptoms are controlled.
Objective: to summarize our experience on postoperative
management of patients of very severe brain injury with
combined therapy of hyperbaric oxygen(HBO). Methods:
retrospectively summarizing 181 postoperative patients of
very severe brain injury from January, 2001 to December,
2005, in our section, all patients were treated by HBO.
Results: of the 181 cases, 5 died (3%), 15 went into
persistent vegetative state (8%), 112 cases were severely
disabled (62%), 49 patients were mild disabled or
completely recovered (27%). Conclusions: the prognosis
of VSBI could be significantly improved on condition that
early, safe, and efficient hyperbaric oxygen treatment
being performed, combined injuries and crucial
complications being prevented and managed in time.
P03
Experimental research on hyperbaric oxygen therapy
against traumatic brain injury
1
1
2
Guo-Hua Wang , Xiang-Gen Zhang , Yong-Cai Li , Yong
2
1
Wang , Zheng-Lin Jiang
1. Nantong University, Jiangsu, China
2. First Hospital of Nantong, Jiangsu, China
P02
Vegetative state and paroxysmal dysautonomia after
brain injury
Qiuyou Xie, Ronghao Yu, Yanbin He, Jin Gu
Hyperbaric Oxygen Medical Centre, Guangzhou General
Hospital of Guangzhou Military Command, Guangzhou,
China
Objective: To analyze the clinical features, nomenclature,
criteria
for
diagnosis,
possible
pathogenesis,
pharmacological
management
and
hyperbaric
oxygenation therapy (HBOT) of vegetative state patients
with paroxysmal dysautonomia after brain injury. Methods:
The clinical presentations, auxiliary examinations,
treatment effect and prognosis were retrospectively
analyzed for 19 vegetative state patients with paroxysmal
dysautonomia after brain injury. Results: Among 19
patients, There were 12 cases of severe traumatic brain
injury, 1 case of cerebellar hemorrhage and received
evacution of hematoma, 1 case of heroin toxic
encephalopathy, 2 cases of severe carbon monoxide
poisoning, 3 cases of hypoxic-ischemic encephalopathy
after cardiopulmonary resuscitation (1 for electrical injury,
1 for coronary angiography and coronary stent
implantation and 1 for cardiac arrest due to anaesthetic
accident). They had most of the symptoms such as
agitation,
hyperthemia,
diaphoresis,
tachypnea,
tachycardia, hypertension, dystonia. No epileptic wave
was found on their electroencephalogram (EEG). Latent
period of physiological waves was prolonged and
amplitude was fallen down on brain auditory evoked
potential (BAEP) and somatosensory evoked potential
(SEP). Varied degree lesions were detected in the cortex,
subcortex, or brainstem by neuroimaging. Most frequently
used drugs were dopamine agonist or antagonist,
benzodiazepines and muscle relaxants. Once their
symptoms were controlled, HBOT could be prescribed for
them. Conclusions: Paroxysmal dysautonomia following
Objective: To observe the therapeutic effect of hyperbaric
oxygen (HBO) against traumatic brain injury (TBI) in rats,
the time window of efficacy and optimal treatment times,
and to investigate the underlying mechanisms of HBO for
TBI treatment. Methods: TBI was induced in rats with the
modified Feeney’s method. For the sham-operated group,
only a bone window was opened without traumatic injury.
An animal hyperbaric oxygen chamber was used to carry
out HBO treatment. Treatment pressure was maintained
at 3ATA of duration 60min with compression or
decompression time of 6min, respectively. The chamber
was kept ventilation with pure oxygen at a flow rate of
2L/min during compression and HBO exposure. The
treatment groups received HBO therapy at 3h, 6h, 12h
and 24h, respectively, after TBI. In addition, at 24h after
TBI three groups of rats received HBO therapy for once,
three, or five times, respectively. The neurological
behavior score, brain water content and Nissl staining
were measured to identify the time window of efficacy and
optimal treatment times. The content of SOD and MDA,
levels of inflammatory cytokines (TNF-α, IL-6, IL-1β and
IL-10) and cell apoptosis (TUNEL method and mRNA of
bax, bcl-2 and caspase-3) in the surrounding brain tissue
of the primary injury were determined to uncover the
underlying mechanisms of HBO therapy. Furthermore, the
expression of the markers of glia cells (GFAP, Vimentin
and S-100) were detected with immunohistochemistry.
Results: In addition to the time point 24h after TBI, HBO
treatment at other three time points 3h, 6h and 12h
displayed significant therapeutic efficacy on TBI, with
decreases of the neurology deficit score, brain water
content and the damage of brain tissue (P0.05).
Compared with control group, HBO treatment at 6h after
TBI elevated the content of SOD and IL-10, reduced the
level of MDA, TNF-α, IL-6 and IL-1β in traumatic brain
tissue. HBO treatment also increased the expression of
bcl-2 mRNA with decrease of the ratio of bax/bcl-2,
reduced the bax and caspsase-3 mRNA and the TUNEL
positive cells. Moreover, the three markers of glial cells all
showed a high expression in the surrounding tissue of the
primary traumatic cortex. Conclusion: HBO exerted a
neuroprotective effect on the brain tissue after TBI, the
efficacy increased when HBO treatment was carried out at
earlier time after TBI. Meanwhile the increase of treatment
times raised the therapeutic effect of HBO at 24h after TBI.
This effect of HBO may be potentially mediated through
following mechanisms: inhibiting the oxidative stress,
reducing the release of inflammatory cytokines, and
suppressing the neuronal apoptosis.
P04
HBO treat 22 cases drowning child
Mao Lixian
Wenlin Hospital, Taizhou, China
Objective: TO study the tread plan and interfering effect
of HBO on drowning child. Methods: sum up our
experiencement on treat total of 22 drowning child with
HBO in 2000-2007. Use large-scale HBO: prepare
medicine etc, keep airway unblocked. Results: 19 cases
(86percent) was recovered, 2 cases leftover sequelae of
nervous system 1cases was dead. Conclusion: HBO
may decrease and inhibit micro-thrombokinesis by to
increase microcirculation blood flow dynamic, to improve
the merphous and function of blood cell and endotbelial
cell. Using HBO for drowning child is distinct improvement
particularly. HBO may decrease the sequelae of nervous
system partianlarly.
P05
HBO treatment of ACOP poisoning delayed
encephalopathy 51 cases
Yang Jingfu
Peoples Hospital of Baodi District, Tianjin, China
Objective: To study the clinical, HBO treatment of ACOP
poisoning delayed encephal opathy. Methods: 51 cases
of patients .HBO treatment,the pressure of oxygen
chamber for the 0.2-0.25mpa,Using the 6 personal small
air pressurized module of the XINYING oxygen chamber
plant HangZhou. Each of the cabin is 60mins,one for a
group of treatment, The number of treatment is 30-60(that
is 3-6 courses). Results: The group of 51 cases cared in
32 cases, Markdely effective in 10 cases, better in 8 cases.
Invalid in 1 cases, the total efficiency was 98%.
Conclusions: For severe ACOP patients, advanced age,
accompanied with hypertension, hypercholesterolemia,
cerebral Infarction or other disease, longer time for coming
round and insufficient HBO treatment might be important
risk factors related to DEACMP. It is scientific and
effective for ws to use mannitol reasonably and to give
ACOP patients sufficient HBO treatment together with
glucocorticoid, medicine of promoting blood circulation
and removing bolld stasis, antiplatelet aggregation agent
and medicine of improving cerebral metabolism.
P06
Hyperbaric oxygen induces endogenous neural stem
cells
to
proliferate
and
differentiate
in
hypoxic-ischemic brain damage in neonatal rats
Yu-Jia YANG, Xiao-Li WANG,
Chong-Feng CHEN,
Qing-Hong WANG, Yue YAO, Meng LI
Division of Neonatology, Department of Pediatrics,
XiangYa Hospital, Central South University, Hunan, China
Objective: Studies suggest that after brain injury,
hyperbaric oxygen (HBO) is neuroprotective by stimulating
cell proliferation. We examine whether HBO promotes
neural stem cells (NSC) to proliferate and differentiate in
neonatal hypoxic-ischemic brain damage (HIBD)
rats. Methods: Seven-day-old rat pups were subjected to
unilateral carotid artery ligation followed by 2 hours of
hypoxia (8% O2). HBO was administered 2 ATA, once
daily for 7 days within 3 hours after HIBD. The proliferating
neural stem cells in the subventricular zone (SVZ) and
dentate gyrus (DG) were dynamically examined by
5-bromo-2-deoxyuridine
(BrdU)/nestin
immunofluorescence. Nestin protein was detected by
Western blot analysis at various time points (from 6 hours
to 14 days) after HIBD. The migrating NSCs were
examined
by
BrdU/doublecortin
(DCX)
immunofluorescence 7 and 14 days after HIBD. The
phenotype of the newborn cells was identified by
BrdU/β-tubulin, BrdU/ glial fibrillary acidic protein (GFAP)
and
BrdU/O4
(oligodendrocyte
marker)
immunofluorescence. Myelin basic protein (MBP) was
examined by immunohistochemistry and pathological
changes of the brain tissue were detected 28 days after
HIBD. Results: In neonatal HI rats treated with HBO, the
proliferation of endogenous NSC was observed in the SVZ
and DG. Cell numbers peaked 7 days after HIBD and
proliferating NSC migrated to the cerebral cortex at 14 d
after HIBD. Twenty-eight days after HIBD, an increase in
newly generated neurons, oligodendrocytes and MBP was
observed in the HBO group compared to the untreated
and HIBD rats. Conclusions: This study suggests that
HBO treatment may promote neurogenesis of the
endogenous NSC in neonatal HIBD rats, contributing to
repair of the injured brain.
Acknowledgments Projects supported by National
Natural Science Foundation of China (the Science Fund of
the Chinese Academy of Sciences), Grant 30672240.
P07
Hyperbaric
oxygen
preconditioning
induces
mitochondrial phenotype to prevent early apoptosis
after spinal cord ischemia in rats
1
2
1
2
Liping Wang , Xuejun Sun , Wenxian Li , Zhimin Kang ,
2
2
2
3
Yun Liu , Hengyi Tao , Weigang Xu , John Zhang
1. Department of Anesthesiology, Changhai Hospital,
Secnod Military Medical University, Shanghai, China
2. Department of Diving Medicine, Faculty of Naval
Medicine, Second Military Medical University, Shanghai,
China
3. Department of Physiology and Pharmacology, Loma
Linda University School of Medicine, Loma Linda,
California, USA
Objective: It is clear that hyperbaric oxygen
preconditioning (HBO-PC) provides spinal cord protection
against ischemia-reperfusion (IR) induced injury. This
study tested hypothesis that HBO-PC induces expression
of endogenous antioxidant enzymes and anti-apoptotic
proteins Bcl-2 resulting in a motor neuronal mitochondrial
phenotype to prevent early apoptosis. Methods: Male
Sprague-Dawley
rats
were
preconditioned
with
consecutive 4 cycles of 1-h HBO exposures (2.5
atmospheres absolute [ATA], 100%O2) at a 12-h interval.
At 24 h after the last HBO pretreatment, rats underwent 9
min of spinal cord ischemia induced by occlusion of the
descending thoracic aorta in combination with systemic
hypotension (40 mmHg). Neurological function, TUNEL
analysis and activities of caspase-3,9 were assessed
during the first day after reperfusion. Mitochondria isolated
from spinal cord of non-preconditioned (control) and
HBO-preconditioned rats were studied before or after
spinal cord ischemia. Results: Spinal cord ischemia
produced marked neuronal death and neurological
dysfunction in animals. HBO-PC enhanced expression of
Mn-superoxide dismutase (Mn-SOD), catalase, and Bcl-2
in the mitochondria in the normal spinal cord at 24 h after
the last pretreatment (before spinal cord ischemia) and
retained higher levels throughout the early reperfusion in
the ischemic spinal cord. HBO-PC decreased superoxide
and hydrogen peroxide levels in mitochondria and
reduced cytochrome c release into the cytosol in the
lumbar spinal cord at 1 h after reperfusion. HBO-PC
attenuated caspase-3 and -9, reduced motor neuronal
apoptosis in the lumbar ventral horn, and improved
neurological function at 24 h after spinal cord ischemia.
HBO-PC increased nitric oxide (NO) production.
L-nitroarginine-methy-ester (L-NAME, 10 mg/kg), a
nonselective nitric oxide synthase (NOS) inhibitor, applied
before each HBO-PC protocol, and abolished these
beneficial effects of HBO-PC. Conclusion: We conclude
that HBO-PC reduced spinal cord ischemia-reperfusion
injury by increasing Mn-SOD, catalase and Bcl-2, and by
suppressing mitochondrial apoptosis pathway. NO may be
involved in this neuroprotection.
P08
Hyperbaric oxygen preconditioning induces tolerance
against brain ischemia
1
2
1
2
Jiasi Li ,
Wenwu Liu ,
Suju Ding ,
Weigang Xu ,
1
3
2
Yangtai Guan , John H Zhang , Xuejun Sun
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical University, Shanghai, China
Objective: The present study examined the hypothesis
that cerebral ischemic tolerance induced by hyperbaric
oxygen preconditioning (HBO-PC) is associated with an
increase of antioxidant enzyme activities. Methods: Male
Sprague-Dawley rats (250-280g, n=74) were divided into
sham, middle cerebral artery occlusion (MCAO) for 90 min,
and MCAO plus HBO-PC groups. HBO-PC was
conducted four times by given 100% oxygen at 2.5
atmosphere absolute(ATA), for 1 hour at every 12 hours
interval for 2 days. At 24 hours after the last HBO-PC,
MCAO was performed and at 24 hours after MCAO,
neurological function and Nissl Staining were performed to
evaluate the effect of HBO-PC. Malondialdehyde (MDA)
content, activities of catalase (CAT), superoxide
dismutase (SOD) and glutathione peroxidase (GSH-px)
sampled from hippocampus, ischemic penumbra or core
of cortex were measured. Results: HBO-PC improved
neurological recovery, lessened neuronal injury, reduced
the level of MDA and increased the antioxidant activity of
CAT and SOD. Conclusion: These observations
demonstrated that an upregulation of the antioxidant
enzyme activities by HBO preconditioning plays an
important role in the generation of tolerance against brain
ischemia-reperfusion injury.
P09
Hyperbaric oxygen prevents ischemia-reperfusion
injury of gut in rats after resuscitation from trauma
and hemorrhagic shock
1
2
2
Gang Wang , Lifang Jin , Weicheng Wang
Hyperbaric Emergency, Chinese Medical University,
Shenyang, China
Background Hyperbaric oxygen (HBO) has been used
therapeutically in intestinal ischemia/reperfusion (I/R)
injury models established by clamping superior
mesenteric artery, with ameliorating intestinal ischemic
damage. This study was designed to investigate the
effects of hyperbaric oxygen (HBO) on the
ischemia-reperfusion (I/R) injury of gut in rats after
resuscitation from trauma and hemorrhagic shock (T/HS)
and elucidate the underlying mechanisms. Methods An
iron mass from a retainable height smashed to the left
thigh of Wistar rat vertically, and at the same time, rats
was bled to establish T/HS model and subsequently
resuscitated with shed blood and normal saline. Just prior
to resuscitation, Wistar rats were randomly divided into 4
groups: sham group, shock group, one HBO treatment
(HBOT) and three HBO treatment group. Results The
levels of lactic acid, induced nitric oxide synthase (iNOS),
nitric oxide (NO), and tumor necrosis factor-α (TNF-α) in
intestinal tissue in one HBOT and three HBOT group after
24 hours from resuscitation were significantly lower than
those in the shock group (P<0.05); The histological injury
grading score of intestinal tissue in one or three HBOT
group was lower than that in shock group and the
difference between them was significant (P<0.05).
Transmission electron microscopic photographs of
intestinal epithelium displayed that microvilli were
hampered and lost, tight junction opened, mitochondrial
crista disappearing, mitochondrium lysis,
rough
endoplasmic
reticulum
expansion,
desmosomes
disappearing etc in shock group; whereas, the tight
junction, desmosomes and mitochondrium appeared
preserved in HBOT groups. All the above-mentioned
indicators of three HBOT group were better than those of
one HBOT group, but the difference was not significant
(P>0.05). Conclusion HBO therapy can improve oxygen
supply to intestine after resuscitation from T/HS, reduce
the anaerobic metabolism, preserved tight junction and
mucosal barrier, and decreased the production of
inflammatory factor after resuscitation from T/HS, inhibit
the excessive inflammatory reaction. Thus HBO prevented
intestine and mucosal barrier from I/R injury after
resuscitation from T/HS.
P10
Kidney dysfunction caused by rat hind limb
ischemia/reperfusion and effect of hyperbaric oxygen
on it
Chun-jin GAO,
Biao YUAN,
Guo-zhong WANG,
Da-zhi CHEN
Beijing Chaoyang Hospital, Beijing, China
Objective: Acute limb ischemia is one of the most
challenging problems encountered by the contemporary
vascular practitioner. Its incidence approximates 1.7
cases per 10,000 population per year. Despite
improvements in patient care and operative technique, the
morbidity and mortality remain high, with mortality rates in
excess of 25% and amputation in 20% of the survivors.
The high rate of morbidity and mortality of acute limb
ischemia should be mainly attributable to the ischemia
/reperfusion itself. Severe hypoperfusion of a limb, if left
untreated, will inevitably develop into tissue infarction and
irreversible cell death. Patients with acute occlusion of the
abdominal aorta are in an extremely compromised state.
The patient's status will likely further deteriorate at the
instant of reperfusion, and sudden cardiovascular collapse
is commonly encountered with the release of accumulated
metabolic by-products into the circulation, which maybe
results in remote multiple organ injury, that is, multiple
organ dysfunction syndrome(MODS). The reason is that
lower limb ischemia/reperfusion injury triggers a systemic
inflammatory response (SIRS, systemic inflammatory
response syndrome). Morbidity and mortality following
reperfusion of the acute ischemic limb may be a
manifestation of multiple organ dysfunction caused by a
systemic inflammatory response triggered by reperfusion
of the ischemic extremities. Hyperbaric oxygen(HBO) has
been used therapeutically in a variety of I/R injury models,
and the application of HBO to I/R disease appears
effective. The basic mechanisms responsible for the
beneficial effect of HBO in treating I/R injury are becoming
better understood. HBO treatment may simultaneously
provide a beneficial effect directed at many components
responsible for I/R injury, including neutrophil,
endothelium, inflammatory mediators, lipid peroxidation,
cellular energetics, and microvascular blood flow. But so
far, there has been no literature on the application of HBO
to kidney dysfunction which results from acute limb I/R.
The purpose of this study is to determine whether there is
potential protective effect of HBO on kidney dysfunction
which is associated with hind limb ischemia/reperfusion in
rat model, and to discuss mechanism of HBO on it.
Methods: Adult male SD rats weighing 220～250g were
used in the study. Animals were kept under standard
condition. They were anesthetized with intraperitoneal
urethane(1.0g/kg) and secured in a supine position on a
heating pad(which is controlled by a model CMA/150
Temperature Controller system); body temperature was
o
kept at 37 C after being shaved. Tracheostomy was
performed with PE-240 tubing(1.67 mm internal diameter),
which allowed spontaneous ventilation with room air .The
left carotid artery was cannulated with PE-50 tubing(0.58
mm internal diameter), and arterial pressure and heart
rate were monitored continuously on a model 7702B
recorder with a 1280 series transducer. The right external
jugular vein was cannulated with PE-50 tubing(0.58 mm
internal diameter)for fluid administration. All animals were
then allowed a 30-minute equilibration period. Intravenous
Ringer’s lactate was administered to maintain the systolic
blood pressure > 90 mmHg throughout the experiment.
The laparotomy was performed. Bilateral hind limb
ischemia was induced by applying an atraumatic clip at
the end of abdominal aorta adjacent to junction site of both
femoral artery. At the end of the ischemic period,
reperfusion of the limbs was achieved by releasing the
clamp. The experimental animals was randomly divided
into three groups, and each group had 10 rats. (1) In
surgical sham group, the animals were subjected to the
operation without clamping abdominal aorta. (2) In
ischemia/reperfusion group, after hind limb ischemia for
2h, reperfusion period was 1h. (3) In HBO group, after
hind limb ischemia for 2h, this group animals were put into
a model DWC150-300 hyperbaric oxygen chamber(HBO
pressure in chamber is 0.2MPa)to be treated for 1h during
1h reperfusion period. The other above groups were put
into the normal pressure air. By using laser Doppler
flowmetry (LDF), the microvascular blood flow was
measured in kidney of the animals. The mean
immunofluorescence intensity of CD31, CD61 or positive
percentage of CD62p were measured by the flow
cytometry(FCM). Ultrastructural histological pathological
changes in kidney were studied. Results: Compared with
I/R group, the microvascular blood flow of the kidney in
HBO group was significantly increased, had significant
statistical difference (P<0.05). Compared with surgical
sham group, the expression of platelet membrane
glycoprotein CD31,CD62p in I/R group was much
higher(P<0.05). The expression of platelet membrane
glycoprotein CD31,CD62p in HBO group was much
decreased than that in I/R group(P<0.05). In I/R group,
extensive ultrastructural histological pathological injury of
kidney occurred. The ultrastructural histological
pathological changes in HBO group was obviously
alleviated. Conclusions: The expression of CD31 and
CD62p on the platelet membrane significantly increased
after rat hind limb ischemia/reperfusion model, indicating
the platelet-activating factor(PAF) was activated, which
partially result in inducing SIRS and remote multiple organ
injury. According to this study, early HBO therapy can
significantly decreased the expression of CD31 and
CD62p on the platelet, indicating the PAF was inhibited,
maybe which stopped a series of PMNL activation ，
adhesion and systemic inflammation after reperfusion of
ischemic hind limb. And the microvascular blood flow of
the kidney in HBO group was significantly increased, the
ultrastructural histological pathological changes such as
kidney in HBO group was obviously alleviated .As a kind
of effective therapy , HBO can alleviate the pathological
changes in kidney dysfunction caused by rat hind limb
ischemia/reperfusion model.
P11
Monitoring and disinfection of air culture in
single-chamber with pure oxygen
1
1
1
2
Xiufang MEI , Chaoqun TU , Xiaomei XIE , Hui LI ,
2
Xiaodan CHEN
1. The Shenzhen Peoples Hospital, Shenzhen, China
2. The People’s Hospital of Longgang Region, Shenzhen,
China
Object: To evaluate the effect of chlorhexidine acetate
solution on single-chamber with pure oxygen and to study
the convenient, effective, rapid and simple routine method
to prevent from cross infection in the single-chamber with
pure oxygen. Methods: There were 5-7 sessions of HBO
in single-chamber with pure oxygen, in which it took
60-80min and interval 10min between 2 sessions. It was
disinfected by spaying with chlorhexidine acetate solution
after HBO. The air culture was done before, after every
session of HBO, after disinfection for 5 consecutive days.
Results: The average total bacterial count (TBC) before
3
first HBO was 189±49cfu/m . The TBC shortly after HBO
3
were more than1500cfu/m . Nevertheless, the TBC after
10min disinfected by chlorhexidine acetate solution were
3
less than 200 cfu/m . Conclusion: It is rapid, simple,
effective with chlorhexidine acetate solution on
disinfection to single-chamber with pure oxygen.
P12
Effects of preconditioning with hyperbaric oxygen on
expression of GFAP and Nestin after spinal cord
injury in rats
Peigang Lu, Hua Feng, Yongzhi Xia, Weihua Chu,
Guoqi Gong
Department of Neurosurgery, Southwest Hospital, Third
Military Medical University
Objective: To study the effects of preconditioning with
hyperbaric oxygen on expression of GFAP and Nestin
after spinal cord injury in rats at different time. Methods:
fifty-five adult Sprague-Dawley adult rats, weighing from
250 to 300g, were randomly divided into 3 groups,
hyperbaric oxygen preconditional group(25,n=5) , normal
injury group(25,n=5)and control group(5,n=5). In the
experimental groups, the rat spinal cord injury models
,
were established by the way of Allen s method and the
expression and proliferation of nestin and GFAP at
different time(1 day, 5 day, 7 day, 10day, 14day) were
observed with immunohistochemical staining and the
analytical system of photographs. Results: In the normal
control group, the expression of nestin was hardly seen
except ependymal cells of central canal, and the low
expression of GFAP was seen. In the experimental groups,
the nestin and GFAP expressions increased obviously in
the injured sites and adjacent sites 1 day to 5 days after
injury, reached the peak value after 7days and followed by
gradual decrease from 10 days to 14 days. There were
statistically significant differences in the nestin and GFAP
expressions between the preconditional group and normal
injury group especially the control group. Conclusion
The above results suggest that spinal cord injury can
induce the expression of nestin and GFAP.HBO
preconditioning can induce the proliferation and
differentiation of the neural stem cells in rats after spine
cord injury.
P13
Observation on curative effect of early hyperbaric
oxygenation to pediatrics brain injury
Guiying He, Guanwen Zhang, Ping Yang, Wenping Han,
Kefang Chen
Xinjiang Kelamayi Central Hospital, Kelamayi, China
Objective: To observe curative effect of early hyperbaric
oxygenation to pediatrics brain injury. Methods: the
clinical data of 51 cases of pediatrics brain injury in our
hospital from 1995,july to 2008.Feb were retro spectively
analyzed. Results: The cure rate of the curative group
and the control group were 78.27% and 52.17%
respectively, the results showed that the control group
were better than those of the control group and the
difference was significant (p<0.05). Conclusion: Early
hyperbaric oxygenation to pediatrics brain injury is an
effective method, which can raise cure rate reduce
disabled rate and improve the prognosis .
P14
Efficacy and nursing technique of hyperbaric oxygen
therapy
on
neonatal
hypoxic-ischemic
encephalopathy
Fei Lianfeng
Capital University of Medicine Affiliated Beijing Children’s
Hospital, Beijing, China
Objective: To explore the short-term effect of hyperbaric
oxygen(HBO)based on conventional therapies in full-term
neonates
with hypoxic-ischemic encephalopathy
(HIE),and the effect of ameliorate sequelaes such as
deafness, cerebral palsy and mental retardation. Methods:
100 full-term neonates with HIE were comprised, 52 were
administered with HBO and conventional therapies, and
the control group comprised 48 patients who were
administered with conventional therapies only. Brainstem
auditory evoked potential and brain CT scan before, 3
months after and 6months after management were
registered respectively. Results: The amelioration rate of
Brainstem auditory evoked potential in treatment group
and control group were 84%, 73% respectively. The
amelioration rate of brain CT scan in treatment group and
control group were 95%, 80% respectively. There is
distinct difference between the two groups (P<0.01). The
therapeutic effect was significant in HBO group than in the
control group. Conclusion: HBO therapy has a marked
short term effect in HIE patients and need to be used as
early as possible. Because of the severity of illness,
rapidness of changes and lack of response, the patients
need to be observed and nursing carefully while taking
HBO.
P15
Recent advance in researches of laboratory and clinic
on hyperbaric oxygen therapy and stem cell
Chen Yifei
Guangzhou City First Peoples Hospital, Guangzhou
Medical College, Guangzhou, China
Hyperbaric oxygen therapy (HBO) is a relatively new
medical treatment that was developed in 1960’s and has
been applied extensively in medical system now. It has
been successfully used in treating a lot of diseases, such
as carbon monoxide poisoning, decompression sickness,
gas Infarction and gas gangrene. There have been
numerous basic and clinical researches on HBO in the
decades. However, the treatment mechanism of HBO
should be explored further and detailedly. Human stem
cell (HSC) is one kind of cell that plays a very important
role in the development and growth of human body and
possesses very strong self-renewal and differentiation
potential. HSC can differentiate into almost all of cell types
of adult body. Compared with traditional therapy,
Application of HSC to treatment of human disease has
many advantages: lower toxin or non-toxin, long effective,
no
immune
repulsion
when
using
self-HSC
transplantation. Therefore, HSC has huge medical value
and extensive application potentials and has been a hot
spot of scientific research in the world now. Reseaches on
HSC are focused in Embryonic Stem Cells, neural stem
cell and hemopoietic stem cell.Recently, a lot of
researches from laboratory and clinic suggested that HBO
could mobilize marrow stem cell into peripheral circulation;
it also could induce proliferation and differentiation of
endogenetic neural stem cell and reduce death of
damaged stem cell. Clinical investigation showed that
HBO could effectively mobilize the hemopoietic stem cell
of peripheral blood. Thus, it is causing tremendous
interest and attention of medical researchers that HBO, a
safety, effectiveness, economy and practicality of therapy,
has action on human stem cell. This article will review the
advance in recent researches on HBO and stem cell,
including 1. biological functions of stem cell; 2. recent
laboratory and clinic researches about HBO impact on
HSC; 3. potential applications of HBO impact on HSC to
clinical treatment of diseases. This article will further
highlight the mechanism of HBO therapy and provide the
newest information of HBO and HSC for medical workers.
P16
Report of a case with serious cerebral air embolism
due to routine hyperbaric oxygen therapy, and
successful rescue
1
1
1
1
Xiaowen pan , shuyi pan , xiangen meng , huijun hu ,
1
1
1
1
2
yu zhang , chen yang , mingxin li , qi zhang , hongbo liu ,
3
wen zhong
1. General Hospital of Navy, Beijing, China
2. Zhuhai People’s Hospital, Zhuhai, China
3. PLA No.422 Hospital, China
We report of a Case with serious cerebral air embolism
due to routine hyperbaric oxygen therapy, and successful
rescue. The patient is female, 44 years. At 2pm, June 3,
2007, the patient entered 12-person-seat air pressure
chamber without oxygen inhalation, she did not feel
abnormally uncomfortable during compression and
stabilizing pressure, and at 3 minutes before leaving
chamber, she suddenly developed dizziness, weakness,
subsequently was unconscious, however, other patients
had no abnormal condition in the chamber. Out of the
chamber, subsequently, her consciousness exacerbated
gradually, which was considered as decompression
disease. She was placed into high oxygen pressure
chamber, with pressure of 0.3MPa, and received oxygen
inhalation, but he patient’s condition was worse, restless,
loss of sight, and right extremity paralysis after she left the
chamber. In this report, we will discuss: (1) Occurring
condition of cerebral air embolism; (2) Differential
diagnosis between cerebral air embolism and other acute
cerebrovascular diseases; (3) Compression treatment of
air embolism; (4) Contraindications for treatment of bullae
and high pressure oxygen.
P17
Research progress of hyperbaric oxygen and free
radical
Jun Wang, Chao Sun
Qingdao Municipal Hospital, Qingdao, China
With the method of documentary data and consulting the
relative literature at home and abroad in recent years, this
paper makes a review on the study progress of Hyperbaric
Oxygen and Free Radical. Respectively, effects of
hyperbaric oxygen on free radicals, hyperbaric oxygen
might reduce the free radical in separately certain
pathology situation, hyperbaric oxygen to be possible to
increase the free radicals in the tumor organization, the
relation of different oxygen exposure doses with free
radicals and the mechanism of hyperbaric oxygen
preconditioning-induced ischemic tolerance in spinal cord
with free radical relational several aspects has been
expounded.
P18
The fast infection and isolation measures in
Hyperbaric Oxygen Therapy rescure procedure for
gas gangrene patients injured by earthquake
Huang Ling-Ling, Chen Shu-Lan, Zhang An-Qin, Nie
Cai-Xian, Tang Ming-Chan, Zeng Yu
The medical science college of Sichuna province, Sichuan
Provincial Hospital, Chengdu, China
Objective: Observe the effect of fast infection and
isolation measures in Hyperbaric Oxygen Therapy rescue
procedure for gas gangrene patients injured by
earthquake. Methods: individual Hyperbaric Oxygen
Chamber into clean area, half-contaminated area and
contaminated area, working staffs wear isolation gown.
During Hyperbaric Oxygen Therapy procedure, disinfect
contaminated objects and floor with 500mg/l chlorine
antiseptic, ultra-violet ray radiation and 3%peroxyacetic
acid fumigation. Disinfect Hyperbaric Oxygen Chamber
repeatedly and take samples after therapy. Results:
patients recovered, no crossing infection occurred, and
chamber samples culture showed no bacteria grow.
Conclusions: crossing infection can be prevented
effectively with strict infection and isolation measures.
P19
The clinical efficacy of hyperbaric oxygen methods in
the treatment of non-insulin-dependent diabetes
mellitus
Shou-Chao LI,
Chun-He LIU,
Yin-Chun ZHANG
People’s Hospital, Weifang, China
Yong-Xin MA,
Objective: To study the clinical efficacy of Hyperbaric
oxygen
(HBO)methods
in
the
treatment
of
non-insulin-dependent diabetes mellitus. Methods: 163
cases of Non-insulin-dependent Diabetes Mellitus patients
were randomly divided into HBO group (87 cases) and
hypoglycemic treatment group (76 cases), we tested
blood glucose, HbA1c, insulin concentration, Cholesterol,
triglyceride, and other indicators respectively in the two
groups after 10 days, 20 days. Results: Blood glucose
and HbA1c levels were significantly decreased in HBO
treatment group after 20 days, there was no significant
difference between HBO treatment group and
hypoglycemic treatment group (p> 0.05). At the same time,
insulin concentration, HDL-C and APO-A levels increased
significantly, and cholesterol, triglyceride concentration
decreased significantly, there were significant differences
compared with drug treatment group (p 1c could
maintained for 20 days with similar levels (p> 0.05).
Conclusion: HBO methods are stable and endurable in
the treatment of diabetic hypoglycemic, it also can
improve the fat metabolism disorder. HBO methods have
the exact effect in the treatment of non-insulin-dependent
diabetes mellitus.
of the people first arrived at high altitude, which was
particular worked by increasing the SaO2 and PO2.
P21
The observation on tinnitus treated with hyperbaric
oxygen and ginaton
Xiang-Hui Li, Yan Li, Xiao-Ying Luo, Hui-Min Sun
General Hospital of Chinese People’s Armed Police
Forces, Beijing, China
Objective: To discuss the clinical value of hyperbaric
oxygen and Ginaton in the treatment of tinnitus. Method:
From January 2002 to April 2008, 106 patients suffering
tinnitus were treated with hyperbaric oxygen and Ginaton.
Pressure of hyperbaric oxygen was 0.2MPa, Breathing
oxygen 60 minutes, One time everyday, One period of
treatment include ten times, The treatment of every patient
is always 10-20 times. At the same time, Ginaton 20ml
transfusion, two times everyday. One period of treatment
is seven days, The treatment of every patient is always
7-14 days. Results: The cure rate, the clearly effective
rate and the total effective rate were respectively 12.3%,
33%, 82.1%(P<0.01). Conclusion: It is suggested that
hyperbaric oxygen and Ginaton have a better effect on
tinnitus.
P20
The effect of air pressure chamber treatment on SaO2
and arterial PO2 of people first arrival at plateau
Chun-yang He, Kai Zhou, Jian-wen Gu, Xi Fu
Chengdu Military General Hospital, Chengdu, China
P22
Therapeutic effects and probable mechanisms of
hyperbaric oxygenation protocol of durative-9-hour
Qiuhong Yu, Yaling Liu, Yali Wu, Lianbi Xu
Department of Hyperbaric Oxygenation, Beijing Tiantan
Hospital, Capital Medical University, Beijing, China
Objective: To study the effect of air pressure chamber
treatment on blood oxygen saturation, and arterial biood
gas analysas of people first arrival at plateau. Methods:
130 new enlisted soldiers were recruited. 65 of them were
enrolled as therapeutic group and the others as control
randomly. The therapeutic group subjects were
trentmented
with
air
pressure
chamber(0.1-0.12Mpa,40-50min ,once a day) after their
arrival at plateau., .The test index included blood oxygen
saturation, arterial, blood gas analysis, morphology of
erythrocyte under the light microscope and anoxia
symptom. Results: After the arrival at Lhasa, some
subjects suffered from anoxia symptom, were faded away
after laser treatment of air pressure chamber. The oxygen
saturation (SaO2)decreased in all subjects, from 98% at
Chengdu airport to 80%(SaO2),PO2 decreased to
50.3mmHg around at the evening of the day after arrival.
The saturation automatically increased from the second
day gradually. But the group with air pressure chamber,
were significantly more than the control (p＜0.01) . At the
end of observation, the SaO2 restored to 97% around in
treated group, and to 90% around in the control group
(p<0.01).The PO2 restored to 83.6mmHg around in the
control group,and to 50.3mmHg around in the control
group(p<0.01). Conclusions: The treatment of air
pressure chamber can increase anoxia-resisting capability
Objective: The aim is to assess the effectiveness of
adjunctive HBOT in the treatment of permanent MCAO
rats for only once continue 9 hours in super-early stage,
and to find one of the probable mechanisms of HBOT.
Methods: (1) models and groups: Using the intraluminal
suture method, we subjected male Sprague-Dawley rats
to permanent middle cerebral artery occlusion model ,
then the rats were randomly divided into MCAO control
group and HBOT group, a sham control group and a
sham-HBOT group also be used. And the groups were
divided into 13h and 5d subgroups. (2) HBOT protocol:
After being modeled for 3 hours, rats in the sham-HBOT
and HBOT groups stayed in the hyperbaric cabin for 9
st
rd
th
th
hours. They inhaled pure oxygen at the 1 , 3 , 5 , 7 and
th
nd
th
th
th
9 hours, and hyperbaric air at the 2 , 4 , 6 and 8
hours. After being created into models, rats in the sham
control group and MCAO control groups breathed room air.
(3) Main Outcome Measures: ① The Garcia neurological
grading systems were used on 3h, 13h, 24h, 3d and 5d to
assess the therapeutic effect of HBOT. ② The infarction
volume was calculated with the TTC pathological staining
on 13h and 5d points. ③ The expression of VEGF was
measured using immunohistochemical stainingon 13h and
5d points. Results: (1)The neurobehavioral outcomes
were improved with time going in all subgroups, there is
significant different between 13h subgroups using Garcia
scale(P<0.01).Also, neurobehavioral outcomes improved
after 13h in 5d subgrops, those of the HBOT group were
more improved comparing with MCAO group, but there is
no different (P>0.05). (2) Cerebral infarction volume of
rats in MCAO group was larger on 5d than that on
13h(P<0.05), and the infarction volume was smaller in
HBOT group comparing with MCAO group on 5d, there is
significant different(P<0.05). (3) The expressions of VEGF
were increased in MCAO groups and HBOT groups.
HBOT groups showed increased VEGF expression
significantly compared with MCAO group on 5d point
(P<0.01). Conclusions: (1) Durative-9h HBOT protecol
was effect, HBOT inproved neurobehavioral outcomes
and cerebral infarction volume, but the effectiveness was
to be further defined. (2) Stimulate the more production of
endogenous VEGF which related with capillary formation,
reduced infarction volume and neural protection is one of
the probable mechanisms of HBOT.
P23
Therapeutic window of hyperbaric oxygen therapy
1
2
1
Xiao-Li WANG ,
Yan-Song ZHAO ,
Yu-Jia YANG ,
1
1
Qing-Hong WANG , Chong-Feng CHEN
1. Division of Neonatology, Department of Pediatrics,
XiangYa Hospital, Central South University, Changsha,
Hunan, China
2. Department of Ophthalmology, Weifang Medical
University, Weifang, China
Objective: Previous studies showed that hyperbaric
oxygen
(HBO)
promoted
cell
proliferation
in
hypoxic-ischemic brain damage (HIBD) neonate rats.
Neural stem cells (NSC) existed in the brain lifelong and
can be activated. This study was undertaken to assess
whether HBO treatment promoted the proliferation of NSC
and repaired the brain damage regardless of when it is
started, thus to explore the therapeutic window of HBO
treatment. Methods: Seven-day-old Sprague-Dawley
rats underwent left carotid ligation followed by 2 hours of
hypoxic stress (8% O2 at 37°C). Hyperbaric oxygen
therapy was administered 3, 6, 12, 24, and 72 hours after
HIBD.
5-bromo-2’-deoxyurindine
and
5-bromo-2’-deoxyuridine/nestin
were
detected
by
immunofluorescence and nestin was examined by
western blot analysis 10 days after HIBD. T-maze forced
alternation, the foot-fault test, and the radial arm maze
were conducted at P 22 days (14 d after HIBD), P 30 days,
and P 34 days. Thereafter, cerebral morphology was
examined by Nissl-staining 28 days after HIBD. Results:
There were remarkable increases in the proliferation of
neural stem cells in the HBO-treated group, 3, 6, 12, and
24 hours after HIBD, as compared with the HIBD group.
The HBO-treated group, 3, 6, and 12 hours after HIBD,
performed better in the behavioral test and had less neural
loss in the hippocampal CA1 region as compared with the
HIBD group. Conclusion: The therapeutic window for
effective HBO treatment could be delayed up to 12 hours
after HIBD, while the effect decreased 24 hours after
HIBD.
Acknowledgments Projects supported by National
Natural Science Foundation of China (the Science Fund of
the Chinese Academy of Sciences), Grant 30672240.
P24
Three phenomenon and the theoretical explanation of
hyperbaric oxygen medicine
Xiao Pingtian
Xiangya Hospital, Central South University, Changsha,
China
In the natural world, it is common that many things can be
divided into three different parts, which are so called
“three-morphous phenomenon”. Moreover, there are
always some inherent correlations and regularities with
these three different forms of the same things. For
example, the quantitative traits have three typic normal
distributions and the objects have three typical movement
modes. With regard to human body, there are three typical
modalities of organs’ connection. “three-morphous
phenomenon” does exist commonly in the field of
hyperbaric oxygen (HBO) medicine, such as tristate
therapy, three states of substances, three stages and
three functions of HBO therapy, three methods of
compression and de-compression, three laws of gas
getting into or out of the body due to the changes of
environmental
gas
pressure.
The
study
of
“three-morphous phenomenon” in HBO medicine will
further enhance our understanding the intrinsic laws of
HBO and benefit the patients for a better therapeutic
effect.
P25
Hyperbaric oxygen therapy in professional athletes
muscle injuries. a series of 51 cases
Philippe Colombani, Olivier Simon, Andréas Kauert, Bern
ard Gamain, Jean-Marc Lapoussière, Marcel Chatel
Nice Hyperbaric Oxygen Center, Pasteur University
Hospital, Nice, France
Background: Traumatic muscle injury affects muscular
fibers, but also conjunctive and vascular tissues. It
provokes lesions similar to an ischemia-perfusion
syndrome, reason why hyperbaric oxygen therapy (HBOT)
has been suggested to contribute efficiently to structural
and functional recovery. However, until now, clinical
studies were conducted only on delayed-onset muscle
soreness (DOMS) and there is no study with statistical
evidence of improvement due to HBOT. Patients and
methods: Fifty-one professional athletes were treated in
Nice Hyperbaric Oxygen Center, among them, 67% were
professional soccer players (mean age 29 +/-10 y).
Wounds occurred during training or competition. They
concerned mostly lower extremities. Muscles injuries were
of various grade: pulled, strained or torned. Beside of
conventional local treatments, HBOT was prescribed
(100% O2 at 2,7 ATA during 45 min; 2 sessions per day).
Mean number of sessions was 12 (range from 1 to 31).
Results: Assessment criteria were clinical, radiological
and functional: 1. Clear reduction of the volume of the limb
and rapid decreasing spontaneous or provoked pain level.
2. Echography or MNR imaging: reduction of size of
hematoma and inflammatory oedema. 3. Shortening of the
resumption of training and quality of muscle strength.
Conclusions: Although this was not a “control cohort”
study, this series suggests that HBOT is apt to bring a
clear-cut and fast improvement in clinical symptoms and
imaging findings after traumatic muscle injury. HBOT
performed in the early stages of intensive sport practice
muscle injury provides a quicker and better quality of
recovery, which is highly important for professional
athletes (and their coach!). Controlled randomized studies
should be done imperatively for confirming these
preliminary results.
P26
Neural protection and toxicity of hyperbaric oxygen
on neonatal rats with hypoxic-ischemic brain damage
Yu-Jia YANG, Bo-Xiang QI, Qing-Hong WANG, Miao JIN,
Meng LI, Yue YAO, Chong-Feng CHEN
Division of Neonatology, Xiangya Hospital, Central South
University, Changsha, China
Objective: It is well known that high-concentration oxygen
(HO) can cause retinopathy of prematurity (ROP) and
bronchopulmonary
dysplasia
(BPD).
However,
high-pressure oxygen (HBO) in the treatment of hypoxic
ischemic brain damage (HIBD) process is also toxic effect
is not clear. The purpose of this study was to investigate
the neuroprotection of HBO and its toxicity on HIBD
neonatal
rats.
Methods:
Seven-day-old
Sprague-Dauleys(SD)rats were randomly divided into six
groups: (1) Control group; (2) HIBD group; (3) HO group;
(4) hyperbaric air group (HA); (5) HBO group; (6)
HO+HBO group. The HA and HBO groups were
administered respectively with 2.0 ATA for 60 minutes of
air or oxygen in HIBD after 2 hrs, once daily for 7 days.
The HO group was given 90% oxygen inhalation after
HIBD for 7 days. The HO +HBO groups were given 90%
oxygen inhalation and HBO daily for 7 days. The animals
were killed on the 21st., Results: Comparison with the
CON group, the number of TUNEL positive cells in
hippocampal CA1 were increased significantly in the HIBD,
HA and HO groups, but not obvious increase in HBO and
HO+HBO. The number of nuclei of the new vessels was
higher in HO and HO+HBO groups. Retinal stretched
preparation vessels in HO and HO+HBO groups
increased significantly, while there were no changes in
evidence in the other groups. Retina VEGF expression
was stronger in HO and HO+HBO group than other
groups. Lung tissue stained with HE showed that the
alveoli pulmonis were damaged obviously in HO and
HO+HBO groups. Conclusions: HBO treatment has
neuroprotective effects on HIBD neonatal rats, but no
toxicity on ocular and lung. Retinal vessel proliferation
induced by high-concentration oxygen was related to the
increasing of the VEGF expression.,
Acknowledgments Projects supported by National
Natural Science Foundation of China (the Science Fund of
the Chinese Academy of Sciences), Grant 30672240.
P27
Expression of MCP-1 in brain of acute CO-poisoning
rats and development after HBO
Xiaoping Xie, Jinsheng Li, Wenlan Wang
Department of Hyperbaric Oxygen Treatment Center,
School of Aerospace Medicine, Fourth Military Medical
University, Xi’ an, China
Objective: To investigate the expression of monocyte
chemoattractant protein-1 (MCP-1) and aggregation of
monocytes/macrophages in brain of acute carbon
monoxide (CO) poisoned rats and to provide new
evidence for immunological mechanism of delayed
neuropathological sequelae (DNS) after CO exposure.
Methods: 50 SD male rats were randomly assigned to
control, CO exposure for 1d, 3d, 7d and HBO treatment for
7d groups, and ten rats for each group. HE,
immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) were performed to
study the expression of MCP-1 and aggregation of
monocytes/macrophages in brain. Results: Expression of
MCP-1 was very low in control group, which increased
quickly after 1d of CO exposure. It reached peak value in
3d exposure group, but decreased after 7d of exposure. In
HBO treatment group, expression of MCP-1 decreased
significantly (P<0.01) as compared with that in 7d
exposure group, but still higher than that in control group
(P<0.05). There was scarcely aggregation of
monocytes/macrophages in brain of control rats. After 1d
to
7d
exposure
of
CO,
the
number
of
monocytes/macrophages-positive
cell
increased
progressively and reached its peak value at 7d exposure
group. Just as the expression of MCP-1, the number of
monocytes/macrophages-positive
cell
decreased
significantly (P<0.01) in HBO treatment group as
compared with that in 7d exposure group, but still more
than that in control group (P<0.05). Conclusions: the
expression
of
MCP-1
and
aggregation
of
monocytes/macrophages may play a role in the
happening of DNS after CO poisoning. HBO may alleviate
the damage of brain through decreasing the expression of
MCP-1
and
preventing
the
aggregation
of
monocytes/macrophages.
P28
Effect of hyperbaric oxygen on acute focal cerebral
ischemia-reperfusion injury in rats
Jinsheng Li, Yaoming Chang, Li Liu, Xiaoping Xie
Department of hyperbaric oxygen treatment center,
School of Aerospace Medicine, Fourth Military Medical
University, Xi’ an, China
Objective: To investigate the effect of hyperbaric
oxygen(HBO) on focal cerebral ischemia-reperfusion (IR)
injury in rats. Methods: Rat model of focal cerebral
ischemia induced by intraluminal filament occlusion of
middle cerebral artery (MCA) was used. Histopathological
changes of infarct size, neuronal damage ， neutrophil
infiltration and NOS positive neurons in the ischemic brain
area after MCA occlusion for 1 hour and reperfusion for
4,11,23 and 71 hours were examined. During the
reperfusion period, normal pressure pure oxygen
(99.5%O2) or 0.25 MPa (ATA) HBO treatment for 1, 2, 3
and 5 times were applied to respective groups and their
results compared. Results: The infarct size, neuronal
damage and neutrophil infiltration in normal pressure pure
O2 group were similar to those of ischemia-reperfusion (IR)
group at 71 hours. Whereas the infarct size and neuronal
damage in HBO group after 5 times of HBO treatment at
71 h were reduced for 8.43% (level before optic chiasma),
11.20%(level of optic chiasma) and 8.66%(level behind
optic chiasma), and the numbers of neuronal damage in 5
high power (×400) fields (Nr/5F) were 11.36 (preoptic
area), 8.94 (medial striatum), and 14.25(cortex) less as
compared with IR group respectively. Besides, the
neutrophil numbers (number/5 fields, 100×) in preoptic,
striatum and cortex at the level of optic chiasma were 3.84,
4.61, 2.56 at 5 h, 6.64, 7.34, 5.72 at 12 h and 9.78, 10.48,
7.84 at 24 h after the IR respectively; and they were much
lower in HBO group at 12 h (5.01, 5.22, 4.03) and 24h
(7.13, 7.21, 6.14) as compared with IR group (P＜0.05,
respectively. The numbers of degeneration NOS positive
neurons were increased gradually after MCA occlusion in
brain area. After HBO therapy, the numbers of
degeneration NOS positive neurons were obviously
decreased in cortex, preoptic area and medial striatum
(P0.05). Conclusions: HBO (0.25MPa, 5 times) have
significant effects of reducing infarct size, lessening
neurological symptom evaluation score, inhibiting
neutrophil infiltration, inhibiting the degeneration of NOS
positive neurons and decreasing the neuronal damage in
“penumbra” area on acute focal IR injury in rats.
P29
Effect of hyperbaric oxygen combined therapy on
vegetative state following cerebral trauma in 124
cases
Ronghao Yu,
Qiuyou Xie,
huai Huang,
Yan Shen,
Yanbin He, Lingling Sun
Guangzhou
general
hospital,Guangzhou
Military
Command, Guangzhou, China
Objective: To study the efficacy and influence factors of
hyperbaric oxygenation (HBO) combining therapy on
patients with vegetative state (VS) following cerebral
trauma. Methods: One hundred and twenty-four cases of
VS treated with HBO combined therapy from 2003 to
2007 were analyzed retrospectively，including the effects
of treatment juncture on therapy and treatment times. The
pressure used for HBO was 0.2 MPa. The patients
breathed pure oxygen for 80 min with an interval of 10 min
in the middle. Once a day, twenty times was a course of
treatment. Results: twenty-two(17.7％)patients were free
from the VS within 1 month. In 102 cases of persistent
vegetative state (PVS), the total effective rate was 90.20%.
Fifty-seven patient regained consciousness (55.9%). Two
groups in which the patients receive HBO oxygenation
after cerebral trauma for less 31 days recovered better the
other one in which the patients receive HBO oxygenation
after cerebral trauma for less 30 days. Conclusion: The
HBO combining therapy is an effective therapy for patients
with persistent vegetative state following cerebral trauma.
The efficacy and regained consciousness was related with
the beginning time of hyperbaric oxygenation.
P30
Effect of early hyperbaric oxygenation on bone
mineral density in spinal cord injured rats
Min Liu, Min Tong, Xiangping Wu
The Second Xiang Ya Hospital of Central-South University,
Changsha, China
Objectives: To establish an animal model of spinal cord
injury (SCI),and to explore the effect of early hyperbaric
oxygenation on bone mineral density (BMD). Methods: 36
healthy
3-month-old female
SD
rats were
randomly divided into three groups: Sham
group
(10
rats), SCI group (13 rats) and SCI+HBO group (13 rats).
Rats in sham group underwent laminectomy, while rats in
the other 2 groups underwent complete spinal cord
transection at the level of the tenth thoracic vertebra. Rats
in the SCI+HBO group were treated with hyperbaric
oxygenation 2th day after SCI ,1/d, 10 times as 1 course
of treatment, for 3 courses, with a 6 days，interval between
courses .All rats were sacrificed 7 weeks after operation,
BMD of lumbar spine (L4-6 ), every region of femur and
tible in vitro were all measured by dual-energy X-ray
absorptiometry (DEXA) using QDR-4500A equipments.
Results: BMD in region 2 at the distal femur ,BMD in
region 6 at the proximal femur and BMD in region 1 at the
proximal tible in vitro were markedly decreased in SCI
group at 7 weeks post-operation compared with that in
sham-operated group (P ＜ 0.05~0.01) ,BMD of every
region at 7 weeks post-surgery in SCI+HBO group
was slightly higer than that in SCI group , but no significant
difference between SCI+HBO group and SCI group was
found . Conclusion: (1) The pattern of osteoporosis in
complete spinal cord transection rats was succeed in
experiment. (2) HBO could not obviously improve bone
loss in complete spinal cord transaction rats.
P31
Comprehensive treatment of neonatal hypoxic
ischemic encephalopathy by sequential hyperbaric
oxygen therapy in the air-pressurized oxygen
chamber: analysis of 257 cases
Xiaoxin Lu, Huiping Peng, Yongjian Tiang, Weihong Fang,
Xinru Hong, Maoying Lin, Chengfeng Wang
Department of Hyperbaric Oxygen Therapy, Fuzhou
General Hospital, Fuzhou, China
Objective: To evaluate the effect of hyperbaric oxygen
therapy (HBO) on the early phase of mandible lengthening
in the goat model. Methods: Six healthy goats were
randomly divided into two groups (n = 3 each). Animals
underwent corticotomy of the hibateral body of the
mandible after placement of distraction devices.
Distraction, at a rate of 1 mm/day and a rhythm of 2
turns/day, began after a 5-day latency period for ten days.
Only the second group underwent HBO for 1.5 hours daily
for fourteen days from the second day of the latency
phase. Twenty-eight days after completion of the
distraction protocol, the animals were killed and the
distracted mandibles were harvested. The appearance of
the samples, radiological changes, bone mineral density,
and mechanical strengths and histology were examined.
Moreover, Serum alkaline phosphatase was measured
before HBO and every four days thereafter from 3 days
pre-treatment till first day post-treatment. Results: 1. In
the experimental group, the surgery region swelling
abated quickly; in the control group, surgery region in one
animal had an infection. Every goat had bony callus, and
the experimental group resulted in harder and wider
healing bone. 2. X-ray: All specimens showed a
progressive calcification of the distracted area between
the mandibular segments, and the bone cerebral cortex
connected completely except the infected animal. The
distracted zone in the experimental group exhibited higher
radiodense opacity. 3. Bone density showed that the
experimental group resulted in a denser healing bone
(P<0.05). 4. Three-point bending test showed that the
maximal loading in the experimental group was higher
than in the control group (P<0.05) .5. In the control group,
three zones exhibited progress of intramembranous
ossification. 6. Statistical analysis of the percentage of
bone trabeculae area in the distraction gap revealed that
experimental group showed a significant increase
compared with the control group (P<0.05). 7. Serum
alkaline phosphatase: early after surgery, every animals
serumal alkaline phosphatase level increased, and in the
experimental group it increased more significantly than in
the control group (P <0.05). Conclusion: HBO can
enhance the anti-infection capacity of the surgery district
and accelerate the rate of bone regeneration and
ossification, and increase the activity of serum alkaline
phosphatase. The domestic internal distractor has a
stable biomechanical property and good biocompatibility.
P32
Clinical study of the impact of hyperbaric oxygen
therapy on hemorheology and neural function
rehabilitation in patients with cerebral infarction
Zhong Qiaofen
Guizhou Provincial Peoples Hospital, Guizhou, China
Objective: To investigate the impact of hyperbaric oxygen
on hemorheology and neural function rehabilitation in
treating cerebral infarction. Methods: Clinical observation
with 48 cases of cerebral infarction treated by HBO
therapy. Compare the preselected indexes and contrast
the condition with those before therapy, then make a
statistical analysis. Results: Compared with the
hemorheological indices before HBO therapy, whole blood
viscosity in high, middle and low shear rate, plasma
viscosity, hematocrit (Hct), erythrocyte aggregation
index(EAI), erythrocyte electrophoretic time(EPT) and
platalet aggregation rate(1,3,M) were more obviously
decreased (p ＜ 0.05-0.01); erythrocyte deformity index
was significantly increased (p＜0.05); both the scores of
erythrocyte sedimentation rate(ESR) and erythrocyte
rigidity index were not statistically significant (p＞0.05).
More over, the average of neurological functional deficit
scales was more obviously decreased (p＜0.01). The total
effective rate was 87.5﹪. Conclusion: Hyperbaric oxygen
therapy is recommendable because of its operational
safety as well as the therapeutic effect in treatment.
Hyperbaric oxygen therapy can not only obviously reduce
the neurological deficit, but clearly improve the
hemorheological properties.
P33
Change of cerebral metabolism rate of glucose in
patient of delayed neuropsychological sequelae after
CO poisoning following combining hyperbaric oxygen
treatment
Yu Gao, Chunjin Gao, Tie Wang, Huan Ge, Lianhua Wu,
Liming Zhao
Beijing Chaoyang Hospital, Beijing, China
Objective: To estimate the effect of hyperbaric oxygen
treatment on cerebral metabolism rate of glucose(CMRglc)
in patients with delayed neuropsychological sequelae after
CO poisoning (DNS). Method: Thirty cases of DNS were
18
F-FDGPET examination before hyperbaric
received
oxygen treatment (HBOT) and after 40th HBOT
respectively. At the same time 17 healthy examiners were
18
also received F-FDGPET examination. The ratio of
mean radioactivity of cerebral lobe to that of cerebellum as
semi-quantitative parameters was used to evaluate brain
glucose metabolism. Result: The CMRglc of DNS patient
decreased in bilateral frontal lobe、parietal lobe、temporal
lobe and occipital lobe before HBOT. After HBOT , there
were obvious improvement of CMRglc in bilateral parietal
lobe、occipital lobe and left side temporal lobe and no
difference in bilateral frontal lobe 、right side temporal lobe.
Conclusion: The CMRglc, decreased, on the early phase
of DNS. Combining hyperbaric oxygen treatment can
improve CMRglc and cerebral function.
P34
Analysis of therapeutic effect of hyperbaric oxygen on
idiopathic facial palsy
Fang Liu, Hua Su, Jia Chen
First Affiliated Hospital, Fujian Medical University, China
Objective: To analysis different degree and different
factors influencing on effectiveness of hyperbaric oxygen
(HBO) on patients with idiopathic facial palsy and to
evaluate the clinical significance of hyperbaric oxygen on
them. Methods: First, the influencing factors were
investigated statistically through a retrospective analysis
of total effective on 196 patients with idiopathic facial palsy,
including sex, age, basic disease, HBO treatment,
house-brackmann facial grading and speed of signal
conduction of facial nerve. Secondly, according to the
different methods of treatment the patients were divided
into group A (routine treatment) and group B(HBO
treatment), compared effectiveness of HBO treatment in
different serious degree.
Results: HBO treatment,
house-brackmann facial grading and speed of signal
conduction of facial nerve were major influencing factors.
The total effective rate of group B was significantly higher
2
than that of group A (x =3.96, P<0.05).No significant
difference was found between A and B group when
house-brackmann facial grading was Ⅱgrade, the same
result when speed of signal conduction of facial nerve was
slight slower. The effective ratio of group B was higher
than that of the group A when house-brackmann facial
grading was Ⅲ~Ⅳ and Ⅴ~Ⅵ grade (P<0.01, P<0.05),
the same result when speed of signal conduction of facial
nerve was middling and serious slower (P<0.01, P<0.05).
The effective ratio of group B was higher than that of the
group A when the delayed treatment time was less than 3
days and more than 31 days (P<0.01). Conclusions: The
curative effect of HBO on different degree of patients with
idiopathic facial palsy is significant particularly in middling
and serious degree patients. Receive HBO treatment
early will help to improve herapeutic effect.
P35
Effect of multiple course of HBO therapy on the
proliferation and differentiation of neural stem cells of
HIBD neonate rats
Qinghon Wang, Chongfen Chen, Men Li, Yue Yao,
Miao Jin, Yujia Yang
Division of Neonatology, Department of Pediatrics, Xiang
Ya Hospital, Central South University, Changsha, Hunan,
China
Objective: To observe whether to increase the
therapeutic course of HBO can promote neural stem cells
(NSC) proliferation and differentiation into neurons and to
improve the efficacy of HBO therapy in HIBD neonate rat.
Methods: Seven-day-old Sprague-Dawley rats underwent
left carotid ligation followed by 2 hours of hypoxic stress
(8% O2 at 37°C). One, two, or three therapeutic courses o f
HBO (Group HBO-1, Group HBO-2, and Group HBO-3)
were administered after 2 hours later (2 ATA, once daily
for 7 days as a therapeutic course). At 35 days, T-maze
forced alternation, the radial arm maze and foot-fault test,
BrdU/nestin and BrdU/NSE immunofluorescence, nestin
protein (western blot) were performed. Results: (1) The
+
+
number of BrdU /nestin cells of SVZ was largest
increases in group HBO-3 compared with that in HBO-1
and HBO-2; (2)There was no obvious increase in
+
+
BrdU /nestin cells of DG in all HBO treatment groups; (3)
+
+
The number of BrdU /NSE cells of brain was largest
increases in group HBO-3 compared with that in HBO-1
and HBO-2; (4) Westen blot showed the express of nestin
protein was highest in group HBO-3 than other groups; (5)
The HBO treatment groups performed better in the
behavioral tests with the increase in therapeutic
courses. Conclusion: To increase therapeutic course of
HBO may promote the proliferation and differentiation of
endogenous NSC in HIBD neonatal rats.
Acknowledgments Projects supported by National
Natural Science Foundation of China (the Science Fund of
the Chinese Academy of Sciences), Grant 30672240.
P36
Acute air embolism with bilateral pneumothorax after
a 24meter dive
Chen Yang, Xiaowen Pan, Xuechang Li
Navy General Hospital, Beijing, China
A diver had an emergency ascent from 24m depth in a
dive accident, and suffered barotraumas with bilateral
pneumothorax and air embolism in CNS. When he was
sent to our hospital 19h after the accident, he was almost
unconsciousness. This is the first case of barotrauma with
bilateral pneumothorax in dive accident in our hyperbaric
center. The patient was successfully treated by
recompression with HBO. It was effective for the air
embolism of the CNS to treat with HBO even after 20h.
P37
Analysis of 156 childhood Carbon monoxide
poisoning managed by hyperbaric oxygen
Zhang Jin
Capital University of Medicine Affiliated Beijing Children’s
Hospital, Beijing, China
Objective: To discuss the characteristics of children
withCarbon monoxidepoisoning (COP), andhyperbaric
oxygen(HBO)therapy,
in
children.
Methods: Carboxyhemoglobin, (COHb) levels were not
parallel with severity of illness .Therapeutic pressure was
due to the age of patient. Mild COP patients need 1~10
times HBO process , moderate COP patients need 10~20
times, severe COP patients need 20~30 times , all patient
got recovery and follow up 2~6 months , no
sequelaes have been found yet. Results: Children COP
manifestations vary a lot, COHb levels can only be a
reference when judging the degree of COP. The prognosis
will be good if HBO can be used as soon as possible and
long enough. Retrospectively analyzed 156 children with
COP treated in Beijing Children’s Hospital HBO
department in recent 3 years. Conclusion: COP occurs in
all ages of children, and can cause variety manifestations.
Infant usually have untypical manifestations.
P38
Experience with non-survivors of acute carbon
monoxide intoxication who received hyperbaric
oxygen therapy
1
2
Te-Chun Hsia , Wan-Yun Hsiao
1. HBO Department, China Medical University Hospital,
Taiwan, China
2. Respiratory Therapy Department, China Medical
University Hospital, Taiwan, China
Purpose: The main purpose of this study was to
determine the reason for the carbon monoxide (CO)
poisoning-related deaths at out hospital and to describe
the demographic data and epidemiology. Methods: We
retrospectively selected this group of acute CO
intoxication patients that received emergency hyperbaric
oxygen therapy (HBOT) from April 2000 to August 2005 at
our hospital. Data regarding age, gender, duration of CO
poisoning exposure, cause of the episode, underlying
disease, number of HBOT courses, hospital course,
cormorbidity and cause of mortality were obtained from
the medical records. We also reviewed the admission data
records, including vital signs, Glasgow Coma Scale,
arterial blood gas, carboxyhemoglobin level, and
intubation or not. Results: One hundred thirty-seven
patients and 5 fatalities related to CO poisoning were
reviewed; the mortality rate was 3.65%. The 5 fatalities
were all male, in the prime of life (27~37 years old), and
without major underlying disease. Four (4/5) patients
committed suicide by inhaling CO from burning charcoal.
They all received emergent HBOT. Prolonged
unconsciousness was noted after series HBOT in 4 (4/5)
patients. Four (4/5) patients developed rhabdomyolysis
and acute renal failure. The causes of death were multiple
organ failure (3/5, 60%) and septic shock (2/5, 40%).
Conclusions: The causes of acute CO poisoning among
the fatalities were suicide by inhaling CO from burning
charcoal (4/5, 80%) and a fire accident (1/5, 20%) at our
hospital. They were in the prime of life without major
underlying disease. The brain is an oxygen dependent
organ, and the damage may be severe and irreversible
after CO intoxication and hypoxia. Rhabdomyolysis and
acute renal failure may also occur. Secondary infection
and septic shock may worsen the already poor condition.
The cause of death may be considered as multiple organ
failure, including the brain, lung, and kidney.
P39
Survival and physiological derangement of rats in the
extreme hypobaric hypoxia environment: Toward a
new animal model of acute mountain sickness
1
2
3
Wang Chia-Ti ,
Niu Ko-Chi ,
Lin Mao-Tsun ,
4
1
1
Chang Ching-Ping , Syong Ci-Huei , Lin Hung-Jung
1. Department of Emergency Medicine, Chi-Mei Medical
Center Tainan 710, Taiwan, China
2. Department of Hyperbaric Oxygen, Chi Mei Medical
Center, Tainan 710, Taiwan, China
3. Department of Medical Research, Chi-Mei Medical
Center, Tainan 710, Taiwan, China
4. Department of Biotechnology, Southern Taiwan
University, Tainan 710, Taiwan, China
Objective: To understand the survival and physiological
derangement of rats in the extreme hypobaric hypoxia
environment.
Methods:
Both
anesthetized
and
un-anesthetized male Sprague–Dawley rats (350-400g)
were exposed to extreme hypobaric hypoxia and,
normobaric normoxia. Hypobaric hypoxia was achieved by
a decompression chamber. The pressure was decreased
to 210 mmHg, which is equivalent to the pressure of 0.27
atm or at 9000m height, in a controlled rate. Survival time
was measured after target pressure achieved and
maintained. The femoral arteries and veins of
anesthetized rats were cannulated for blood pressure,
MAP and HR monitoring, and the arterial partial pressure
of CO2 (Paco2), O2 (Pao2), SatO2, HCO3 and PH value
were measured via a blood gas analyzer. The brain
neurotransmitters
expression
was
analyzed
via
stereotaxically implanted microdialysis probe, and lactate,
glycerol, pyruvate and glutamate levels were measured.
The blood-brain barrier (BBB) expression and brain
edema were measured by Evans blue method and brain
dry/wet ratios respectively. We compared the extreme
hypobaric hypoxia group and normbaric nomoxia group
for understanding the difference in physiological
characteristics. Result: The average survival time was
29±5.4 min (n=10) for un-anesthetized rats; and 26±6.5
min (n=10) for anesthetized rats in our extreme hypobaric
hypoxia setting. The arterial partial pressure of CO2
(Paco2), O2 (Pao2), SatO2, HCO3 and PH value
significantly decreased, and the neurotransmitter
expressions in brain such as glycerol, glutamate and
pyruvate/lactate ratio were significantly increased in
extreme hypobaric hypoxia. We found no significant
difference in the blood-brain barrier (BBB) expression and
brain dry/wet ratios in our study. Conclusion: We report
the survival time and provide the evidence of changes in
physiological characteristics and brain neurotransmitters
of rats in extreme hypobaric hypoxia environment. The
development of a new animal model for rats under
extreme hypobaric hypoxia is advantageous for academic
researches for diseases related to hypobaric hypoxia,
such as acute mountain sickness.
P40
A thermodynamic mechanism for explaining the
concurrence of joint ache and neurological deficit in
one limb
Cristian Melián
Asociación Chilena de la seguridad, Puerto Montt, Chile
Objective: To suggest a physiopathological mechanism
that explains the clinical observation of spinal
cord-articulation congruency. Materials and methods: a)
Revision of records b) Analysis of Data. Results:
Thermodynamic hypothesis of probability of bubbles
appearance in a tissue subjected to mechanical energy:
“For any heterogeneous open thermodynamic system,
with non-adiabatic walls constituted by several
non-compressible phases, with inert gases dissolved in
them and subjected to pressure, the probability of
formation of gas bubbles in the system when the pressure
diminishes is directly proportional to the total energy
(determined by factors such as thermal energy and mass
of gas) accumulated by the system during the period of
time in which it was subjected to pressure; and inversely
proportional to the speed of energy transfer from the
system into the environment in the form of heat or mass of
gas”. The specific place of bubble formation will be the
phase presenting a greater ∆E and its surroundings. The
skeletal muscle system, in the four limbs, receives a
similar mass of gas. The diver applies work to the skeletal
muscle system; for example, to the right arm: kinetic
energy is generated which turns into an increase of
internal energy and heat in the limb. The longer the time
the action is performed the greater the energy load and
mass of gas. When the diver ascends the pressure
diminishes, the speed of elimination of gas mass and heat
is surpassed in the right arm, secondary to the energy
accumulation during the task, with bubbles appearing in
tissues with a greater ∆E and in its surroundings; for
example, in the right shoulder. In the spinal cord,
specifically in the synapse of the 1st and 2nd
motor-neurons in the right arm, a greater amount of heat
and gas mass accumulates, the elimination speed rate is
surpassed and bubbles appear. The specific phase for the
spinal cord will then be the inter-synapse space and its
surroundings. The bubbles would cause a mechanical
blockade of the nervous stimulus that would be
transformed into a sensitive or motor deficiency. So, the
degree of neurological deficiency would be given by the
number of synapses involved. Neurological recovery
would be given by: number of synapses involved, quantity,
size and time the bubbles interact in the synapses,
passing from a reversible mechanical-physiological
dysfunction to an irreversible anatomical alteration.
P41
Decompression sickness (DCS)-Is there a relationship
between the anatomical location of joint pain and
location of injuries in spinal cord?
Cristian Melián
Asociación Chilena de la seguridad, Puerto Montt, Chile
Summary: A retrospective, cross-sectional and
descriptive investigation was realized in 35 patients with
DCS Type I (joint pain) and Type II (neurological deficit) in
the same episode of DCS. The patients were treated in
“Unidad de Baromedicina ACHS Puerto Montt (Chile)”, in
the period between March 2006 and March 2008. A
registry of the anatomical location of joint pain and
neurological deficit was realized, dividing the body in 4
quadrants. There were defined four types of relation of
congruence. 1) Congruence of quadrant 2) Congruence of
side 3) not demonstrable Congruence 4) there is not
congruence of quadrant. Objective: To describe the
existence of a relation between the anatomical location of
the joint pain and the location of the lesion in spinal cord
(spinal cord - articulation congruency). Materials and
Methods: It made a review of records and analysis of data
obtained from each patient. Results: A congruence of
side (spinal cord - articulation congruency) was observed
in the 100% of the cases, in which it possible
demonstrated positively or negatively congruence. In 80
% of these cases the congruity Medular - articulate is in
the same quadrant. In 0% of the cases “there is not
quadrant congruence”. Conclusions: A relation between
the anatomical location of the joint pain and the location of
the lesion in spinal cord was observed.
P42
Activation of FGF-2/MEK/ERK 1/2/NF-κB and PKC/JNK
Pathways by Hyperbaric Oxygen Stimulated
proliferation in Osteoblast
1
2
CHEN-PU HSIEH , CHING-YUANG LIN
1. Orthopaedic Surgery Department and Hyperbaric
Center ,Changhua Christian Hospital, Taiwan, China
2. Institute of Medical Research, College of Health
Sciences, Chang Jung Christian University, Tainan,
Taiwan, China
Background: To investigate whether the hyperbaric
oxygen(HBO) can promote the growth-arrest osteoblast
(OB) cells to proliferate and differentiate, and to determine
the probable mechanism .Methods: OB cells were
exposed to O2 with different levels of saturation and
pressure for 3 days and 7 days. The OB cells were divided
into four groups: 1.TheControl Group (Group C ):cells
were cultured under ambient oxygen (21% O2) and
normal pressure(1ata). 2.ThePressure Group(Group
P) :cells were treated with high pressure(2.5ata) only twice
daily. 3.High Oxygen Group(Group O): Cells were treated
with high concentration oxygen(50%) only twice daily.
4.Pressure and High Oxygen group(Group P+O): Cells
were treated with high pressure(2.5ata) and high
concentration oxygen(50%) twice daily. XTT was used to
detect the cells proliferation and cell cycle progression
was determined by Flow analysis. Expression of growth
factors was assayed by RT-PCR. In addition, we
determined HBO activated signaling pathway in OB cells
by Western Blot analysis. Results: HBO therapy did
significantly promote OB cell proliferation and stimulated
cell cycle progression after the cells had been treated for
three days.Afterward, the effect attenuated day by
day. HBO also stimulated the OB cells to produce the
FGF-2 growth factors. Multiple signaling pathways,
s6k
including FGF-2/MEK/ERK 1/2/Akt/p70
/NF-κB and
PKC/JNK, are involved in the proliferation of OB cells by
HBO stimulation .Conclusions: The pressure and high
saturation of pressure have positive effects on stimulating
grow-arrested OB cells to proliferate and differentiate
s6k
through activation of FGF-2/MEK/ERK 1/2/Akt/p70
/NF-κB and PKC/JNK signaling pathway.However,we
were unable to determine between air pressure and
oxygen concentration which is the more important factor.It
is believed that HBO can be useful for fracture healing in
clinical application in an optimal situation.
P43
Hyperbaric oxygen therapy for chronic refractory
osteomyelitis of the sternum: a case report
Raymond
C Shields, Francis
C Nichols, William
G Buchta, Paul L Claus
Mayo Clinic and Mayo Foundation, Rochester, MN, USA
Objective: To review a case of chronic refractory
osteomyelitis of the sternum. Methods: A 32-year-old
female with a history of lye ingestion at 14 months of age
underwent multiple esophageal surgical procedures
including subcutaneous colonic interposition with
conversion to substernal colonic interposition requiring
median sternotomy, revision of pharyngocolostomy, and
eventual revision of cologastric anastomosis for
obstruction. The last surgical procedure was complicated
by a nonhealing draining sinus tract at the sternum.
Clinical and computed tomography findings were
consistent with chronic sternal osteomyelitis without
anastomotic leakage. Narcotic analgesics were frequently
required for pain management. Hyperbaric oxygen
therapy (HBOT) was initiated. Twenty four of 40
recommended sessions were completed which included a
profile of 100% oxygen administered via an oxygen hood
(Sea-Long, Series 500 Hood / Neckdam System,
Louisville, KY, USA) at 105 kPa (2 atmospheres absolute,
33 fsw) for 90 minutes with a 5 minute median air break.
All sessions were conducted in a multiplace chamber
(Fink Engineering TL24-165 AH11, Queensland,
Australia). Ertapenem antibiotic therapy was continued
throughout HBOT and discontinued at completion. No
surgical debridement was required. Results: Dramatic
pain relief was seen early along with cessation of sternal
drainage. Upon completion of 24 sessions, the sternal
wound had healed completely. The C-reactive protein
improved from 16.1 mg/L before HBOT to less than 3
mg/L at completion. Conclusion: HBOT is beneficial for
chronic osteomyelitis by reversing tissue hypoxia,
enhancement in leukocyte bacterial phagocytosis, and
improving tissue perfusion. These mechanisms appear to
have been important in the healing of chronic sternal
osteomyelitis in this patient. Reports of HBOT for sternal
osteomyelitis remain limited in the English literature.
Further investigation with well-designed clinical trials of
HBOT for this indication is necessary to substantiate this
treatment
P44
Hyperbaric oxygen and concurrent chemotherapy:
what are the concerns?
John Feldmeier
University of Toledo Medical Center, Radiation Oncology
Department, Toledo Radiation Oncology, USA
Objective: To review the published literature and putative
effects of cancer -chemotherapy on patients receiving
hyperbaric oxygen. Methods: Electronic computer data
base literature searches as well as manual searches of
known references in hyperbaric medicine were employed
in order to define the potential interactions of hyperbaric
oxygen and chemotherapy in cancer patients undergoing
active treatment. In the past decade several new drugs
with new mechanisms of action have become available.
These drugs may target growth factors such as VEGF
(vascular endothelial growth factor) or EGF (epithelial
growth factor) while traditional cytotoxic chemotherapy is
designed to directly disrupt cancer cell proliferation by
interfering with mitosis. Results: The interaction between
hyperbaric oxygen and cancer chemotherapy is complex
and not well-studied. Older recommendations that
absolute contraindications exist for most traditional drugs
are not justified by available published literature. For
newer drugs targeted against growth factors, the
mechanism of action suggests that they will inhibit
hyperbaric oxygen’s salutatory effect on wound healing.
More study is needed before definite recommendations for
these new drugs can be made. The review of these issues
suggests that prior concerns regarding traditional cytotoxic
chemotherapy are not justified by the published
experience. Special considerations do relate to Bleomycin
where reports document occasional but potential life
threatening pulmonary fibrosis in patients exposed to
oxygen-enhanced breathing gasses even some time after
Bleomycin administration. Some drugs may be potentiated
in their cytotoxic effects on cancer cell growth though this
application has not been widely investigated or adapted to
clinical advantage. For some of the newer drugs such as
Bevacizumab and Cetuximab, there is not yet an
adequate published experience to make definite
recommendation; however, based on their mechanisms of
action, these and other drugs with similar mechanism are
likely to interfere with the hyperbaric oxygen delivered to
enhance wound healing or treat delayed radiation injuries.
Conclusions: The interaction between hyperbaric oxygen
and cancer chemotherapy is complex and not well-studied.
Older recommendations that absolute contraindications
exist for most traditional drugs are not justified by
available published literature. For newer drugs targeted
against growth factors, the mechanism of action suggests
that they will inhibit hyperbaric oxygen’s salutatory effect
on wound healing.
P45
Analysis of dynamic state of rheo-encephalogram
during the procedure of hyperbaric oxygen therapy
1
1
1
Hongjun Wang ,
Qibiao Weng ,
Lingling Chen ,
1
2
1
1
Xuelin Chen , quyun Yang , Jin Liu , Guimei Zhou
1. Zhujiang Hospital attached to Southern Medical
University, Department of Hyperbaric oxygen, Guangzhou,
China
2. The First Hospital attached to Guangdong
Pharmaceutical College, Guangzhou, China
3. Shilong People’s Hospital of Dongguan City,
Guangdong Province, Guangzhou, China
4. The Second People’s Hospital of Nanhai District,
Foshan City, Guangdong Province, Guangzhou, China
Objective: Three dynamic states of rheo-encephalogram
(before HBOT, during HBOT and after HBOT) of patients
who received the HBOT (hyperbaric oxygen therapy) were
analyzed, so as to observe and analyze the effect of the
whole procedure of HBOT on function of cerebral vessels,
to know about the diversity and rule of function of cerebral
vessels and change of cerebral blood flow, and to provide
theory of HBOT on cerebral diseases. Method:
Rheo-encephalogram was done in the following states: in
normal atmospheric pressure, in 0.2 MPa, after
40-minute’s HBOT, in normal atmospheric pressure after
the whole procedure of HBOT. All data is analyzed by
SPSS 11.5. Result: Differences are significant in the
following items: resistent index, rise time, whole recovery
time, elasticity index, increased angle, top included
angle. Items of rheo-encephalogram of internal carotid
artery and vertebrobasilar artery are dissimilar between
“patients after inhale oxygen, patients go out the
hyperbaric chamber” and “before pressure is elevated,
after pressure is stable”, while differences are not
significant between “before pressure is elevated” and
“after pressure is stable”, so do “patients after inhale
oxygen” and “patients go out the hyperbaric chamber”.
Conclusion: HBOT plays a positive role in vascular
resistance and angiotasis when HBOT was received,
while HBOT exerts no influence on volume of blood flow of
cerebral vessels. It is indicated that change of vascular
resistance and angiotasis is mainly affected by stimulation
of high pressure oxygen on vascular smooth muscle
rather than elevated oxygen pressure. Advanced research
is required on cause of cerebral vessels’ contraction
without reduction of volume of blood flow and duration of
effects of elevated oxygen pressure on function of
cerebral vessels.
P46
18F- FDG PET imaging before and after hyperbaric
oxygen combined therapy in persistent vegetative
state
Yu Ronghao, Xie Qiuyou, He Yanbin,et al.
Department of Hyperbaric Oxygen, Guangzhou general
hospital, Guangzhou Military Command, Guangzhou,
China
Objective: To evaluate the therapeutic effect of
hyperbaric oxygen combined therapy in persistent
18
vegetative
state,
(PVS)
patients
with
F18
fluorodeoxyglucose ( F- FDG) position emission
tomography (PET) imaging. Methods: Ten patients with
18
PVS received F- FDG PET imaging examination before
and after hyperbaric oxygen combined therapy. Results:
All of the 10 cases were with abnormal cerebral glucose
metabolism. Some abnormal cerebral glucose metabolism
areas were concordant with the changes on brain CT or
MRI,, but some were not. As PVS recovered,
improvement of cerebral glucose metabolism or complete
18
return to normal were also observed., Conclusions: FFDG PET imaging is a safe and reliable modality, which
not only can directly reflect the level of cerebral cerebral
glucose metabolism and serve as a supplementary work
up to brain CT and MRI, but also can be used to evaluate
the therapeutic effect of hyperbaric oxygen combined
therapy in PVS and to predict the prognosis.
P47
Combined treatment of hyperbaric oxygen therapy,
acupuncture and rehabilitation in four cases of
neurological decompression sickness
Hui-Chieh Lee
Zuoying Armed Forces General Hospital, Taiwan, China
Objective: The neurological decompression sickness
(DCS) may cause severe symptoms and sequelae such
as paraplegia and difficulty in, micturition and defecation.
Traditional treatments includes United, States Navy (USN)
treatment tables, hyperbaric oxygen (HBO2) therapy, &
rehabilitation. In order to improve the therapeutic effects,
we, tried to use a combined treatment of HBO2 、
acupuncture &, rehabilitation in the neurological DCS .
Methods: Four cases of neurological DCS were collected,
via our emergent room and diagnosed by means of diving
history,,
clinical
manifestations,
and
adjunctive
examinations
such
as,
hemoglobin
level(Hgb), hematocri(Hct), computerized tomography,
(CT), and magnetic resonance image (MRI). All cases
received the, combined treatment :the USN treatment
table 6A and subsequent, HBO2 therapy (50 ft/ 120 min);
acupuncture of head, neck, hand, abdomen, back, buttock,
leg and blood letting of Wei-Chung, acupuncture point and
physical therapy. Results: Four male fishermen had a
repetitive dives to the depth, (25-52 m) for 40-100 min with
SCUBA or surface supply compressed air., All dives
exceeded no decompression limit without adequate,
decompression stop . The clinical symptoms included
changed, mentality, ptosis of eyelid, numbness and
weakness of upper limbs or, lower limbs, difficulty in
urination and defecation. Two cases had increased Hgb
and Hct. One CT scan showed the brain edema and two
had, positive MRI findings of brain and cervical spine.
After the combined, treatment, three cases (75%) had
complete recovery of motor function, and walked smoothly
leaving the hospital. One serious case could walk, for a
short distance with the walking stick. Hypoesthesia over
lower, trunk improved markedly. Two cases had urination
by themselves and, smooth defecation. The other two had
sensation of urination and, increased power of anal
sphincter but still needed urinary catheter, and laxative.
The hospital stay was all within 30 days for four cases.
Conclusion: From these clinical results, we suggest that
this, combined treatment of acupuncture, HBO2 and
rehabilitation may be, another useful choice in the
management of neurological DCS.
P48
Mechanically ventilated patients poisoned with
carbon monoxide: outcome after hyperbaric oxygen
therapy
1
1
1
Wang Chia-Ti ,
Cheng Chin-Jen ,
Liu Te-Ming ,
2
Niu Ko-chi
1. Department of Emergency Medicine, Chi Mei Medical
Center, Tainan, Taiwan, China
2. Department of Hyperbaric Oxygen, Chi Mei Medical
Center, Tainan, Taiwan, China
Objective: To review the outcome of severely poisoned
patients who receiving mechanical ventilation and HBO
therapy. Methods: We reviewed the case records of 16
patients required mechanical ventilation for severe carbon
monoxide poisoning treated with HBO at Chi-Mei Medical
center between May 2001and December 2005. We
obtained numbers of days admitted in ICU and wards,
underlying diseases, laboratory data. We analyzed the
mortality and the morbidity in these treated patients. Only
patients without any other comorbidity, like trauma or burn
injury, can be included in our study. Results: Fourteen
(87.5%) of 16 patients committed suicide by burning
charcoal, 1(6.25%) was poisoned by car exhaust and
1(6.25%) was poisoned by faulty water heating
installations. All patients received endotracheal tube
insertion before HBO therapy and HBO therapy
subsequently. Although 15 patients received HBO therapy
th
within 2 days after admission, one was treated on the 16
day after admission due to his unstable condition. Mean
ICU stay was 7 days and total hospital stay was 16.7 days.
There was 1 mortality case, and the other three were also
defined as poor outcome due to their vegetative status.
Twelve (75%) patients who underwent HBO therapy were
discharged without severe sequelae. Conclusion:
According to our experience, mechanically ventilated
CO-poisoned patients who receive hyperbaric oxygen had
a fair prognosis. Although the risks for the mechanically
ventilated patients are relatively high, HBO therapy
seemed to reduce the risk of serious neurological deficit in
severe CO poisoned patients.
P49
Experience in the treatment of decompression
sickness from the mountain lake diving in Taiwan
Shyanher WANG
Department of Occupational and General Medicine,
Tungs’ Taichung MetroHabor Hospital, TaiZhong, Taiwan,
China
Decompression sickness (DCS) occurred in the mountain
lake is extremely rare in Taiwan., However, there were 3
victims during a 6-day rescue dive after the Mindulle
typhoon attacks., It damaged the water gate in the Carp
Reservoir where located 300 meters above sea level.
Eleven male participated the dive., Their average age,
body weight and diving experience were 25 year-old, 72
kg and 3 years, respectively., The rescue started 24 hours
after ascending., The surface-supply dive was adopted
due to the limited time and space., The bottom time was
30 minutes and the depth 60 meters under the mountain
lake., Two dived each time. The patients were treated with
the modified and extended recompression tables
according to the individual condition. One victim with bend
over multiple joints was treated with a modified table 5A.,
He got complete remission after the 5-hour treatment.,
The other two victims with bend and numbness over
multiple limbs were treated with a modified table 6A for 8
hours., Because both had some residual symptoms, a
table 5 was added on the next day., Both got complete
remission thereafter., All three victims received no other
medicines except of isotonic saline., None of them has
late complication to date. The relative high incidence of
DCS in this mountain lake dive might be from the hostile
environment. The modified table 5A and table 6A, added
with a followed table as necessary, might be tailored to
optimize the treatment of the DCS occurred in the
mountain lake.
P50
Hyperbaric oxygen treatment of bone marrow edema
(BME) and avascular osteonecrosis (AVN) of knee
joint in comparison to talus. first long term results (5
years) in therapeutic outcome
Johannes von Reumont, Anke Fabian
Hyperbaric Centre, Heidelberg, Germany
Objective: Positive effects in the BME and AVN treatment
of the knee joint were first-time observed during a pilot
study in our centre, in 1998 and 99. These results entailed,
to a prospective clinical study to evaluate the therapeutic
benefit of hyperbaric oxygen therapy (HBOT) in a large,
patient population. Moreover a, first five years follow-up
study, should give a prediction for the stability of the
results after therapy. Method: Within the past 8 years 266
patients with MRI confirmed diagnosis of BME or AVN
were evaluated. They were classified according to the
Association for Research of Circulation Osseous (ARCO),
knee joint (n = 207) and ankle joint, (n = 59). Clinical
examination, (range of movement , pain score) was done
before, during and after therapy and within the follow-up
period. MRI control after, HBOT was classified for results.
The number, of HBOT sessions (250/60) varied,
according to, the clinical, success (minimum 15 or more).
Classification of Result: 3+ no complaints/ 2+ clear
approvement 1+ less 0 no progress. In the follow-up study
group (FSG) n = 104, patients with interaction of
interfering factors (e.g. operation, arthrosis) were
excluded to evaluate, the real effect of HBOT stability (n =
64). Results: Mean age knee group (KG) was 57 years in
comparison to the talus group (TG) 40 years. Number of
sessions 27(KG) and 25 (TG) Clinical outcome ARCO
stage 2, KG: 77,2% rated 3+, 19% rated 2+ in comparison
with outcome stage 2 TG: 64,3%, 3+, 28,6 2+. Clinical
outcome ARCO stage 3 knee group 60% 3+ versus 42,2%
TG. The MRI outcome varies significantly in this stage: 3+
rated in 38,4% versus 9,1% TG. 94 % in the FSG
answered our questionnaire. 81% remained in an
unchanged therapeutic outcome, improvement in 6% and
symptomatic aggravation also in 6%. Most of the changes
were, seen within the first year. Conclusion: HBOT
administered as a conservative causal therapy leads to
very good results in earlier ARCO stages particularly, in
the knee joint. There is a significant difference in MRI
rating in stage 3. The therapeutic follow-up outcome
already shows very stable results within the first year.
P51
Effect of hyperbaric oxygen therapy on bone
regenerating
ossification
during
mandibular
distraction osteogenesis in goats
1
1
2
Mingxing Li , Xiaoxin Lu , Jie Zheng
1. Department of Hyperbaric Oxygen, Fuzhou General
Hospital, Fuzhou, China.
2. School of Stomatology ， Fujian Medical University,
Fuzhou, China
Objective: To evaluate the effect of hyperbaric oxygen
therapy (HBO) on the early phase of mandible lengthening
in the goat model. Methods: Six healthy goats were
randomly divided into two groups (n = 3 each). Animals
underwent corticotomy of the hibateral body of the
mandible after placement of distraction devices.
Distraction, at a rate of 1 mm/day and a rhythm of 2
turns/day, began after a 5-day latency period for ten days.
Only the second group underwent HBO for 1.5 hours daily
for fourteen days from the second day of the latency
phase. Twenty-eight days after completion of the
distraction protocol, the animals were killed and the
distracted mandibles were harvested. The appearance of
the samples, radiological changes, bone mineral density,
and mechanical strengths and histology were examined.
Moreover, Serum alkaline phosphatase was measured
before HBO and every four days thereafter from 3 days
pre-treatment till first day post-treatment. Results: 1. In
the experimental group, the surgery region swelling
abated quickly; in the control group, surgery region in one
animal had an infection. Every goat had bony callus, and
the experimental group resulted in harder and wider
healing bone. 2. X-ray: All specimens showed a
progressive calcification of the distracted area between
the mandibular segments, and the bone cerebral cortex
connected completely except the infected animal. The
distracted zone in the experimental group exhibited higher
radiodense opacity. 3. Bone density showed that the
experimental group resulted in a denser healing bone
(P<0.05). 4. Three-point bending test showed that the
maximal loading in the experimental group was higher
than in the control group (P<0.05) .5. In the control group,
three zones exhibited progress of intramembranous
ossification. 6. Statistical analysis of the percentage of
bone trabeculae area in the distraction gap revealed that
experimental group showed a significant increase
compared with the control group (P<0.05). 7. Serum
alkaline phosphatase: early after surgery, every animal's
serumal alkaline phosphatase level increased, and in the
experimental group it increased more significantly than in
the control group (P <0.05). Conclusion: HBO can
enhance the anti-infection capacity of the surgery district
and accelerate the rate of bone regeneration and
ossification, and increase the activity of serum alkaline
phosphatase. The domestic internal distractor has a
stable biomechanical property and good biocompatibility.
P52
Breath hold apnea tables for lower embolism (BATLE)
experience after more than six years 2000 – 2008
John M Batle
MEDISUB Hyperbaric & Underwater Research Institute
Palma de Mallorca, SPAIN
In the Balearic Islands, there is a great number of people
interested in submarine fishing. Although fish are
becoming scarcer to find, and the bigger ones are at a
deeper depth which leads to the use of electric scooters
( submarine scooters) arriving to depths of up to 63 metres
in apnea in the space of approximately two minutes to the
said pressure with intervals on the surface of about two or
three minutes.The number of dives per hour is about
fifteen and each sesión is usually done over a period of
between three to eight hours. Materials and Methods:
Since l994 to present date, a study has been made of
about forty cases of disbaric diving accidents in
apneaatended by Medisub Hyperbaric Institute during the
period l995 -2005. In the year 2000, the first tables of
Breath Hold Apnea?Tables?for Lower Embolism ( BATLE )
were published at the EUBS Congress in Malta, and the
second generation of tables were published in ICHM 2002.
Now with more than six years of practise and experience
of submarine fishing with the said tables. Results: The
number of accidents diminished progressively and since
then, few accidents have been presented in divers
practising submarine fishing.
Conclusion and Discussion: The extensión of the time
the divers spend on the surface after each dive, thanks to
the conclusión of Breath Hold Apnea Table for Lower
Embolism has made accidents due to the formation of
bubbles caused by the excess of nitrogen accumulated in
the time of diving almost extinct.
P53
Summarization of 3180 children with hyperbaric
oxygen therapy
Chen Yanhua, Capital University of Medicine Affiliated
Beijing Children’s Hospital, Beijing, China
Objective: Hyperbaric oxygen department of Beijing
Children’s Hospital was founded in 2001-6-1. It has been
7 years since then. Our department has become the
biggest pediatric HBO unit in Beijing region. Now we have
2 adult pure oxygen chanbers, 5 infant chanbers. There
are 50 person-times per day on average and now we have
treated over 70000 person-times safely, including more
than 10 kinds of disease. Methods: Now we summarize
our experiences of management of all kinds of disease in
children
as
follows:
Neonatal
hypoxic-ischemic
encephalopathy, Cerebral paralysis, Zentrale koordination
storung, Virus encephalitis, Carbon monoxide poisoning,
Virus encephalitis combined with cortex blindness,
Congenital heart disease after operation, Exterior
waterhead, Sudden deafness, Brain trauma, Toxicosis
encephalopathy ,crushing syndrome etc. Age: the
youngest patient is 8 hours after birth, the oldest is 18
years. Therapeutic scheme: Therapeutic pressure range
from 0.05MP to 0.1 MP. Result: According to our
experiences, pediatric HBO is a valuable method for most
cases, especially for those Carbon monoxide poisoning,
Virus encephalitis combined with cortex blindness,
drug toxicosis, HIE,
crush
syndrome
and
virus
encephalitis. Conclusion: We consider that pediatric
HBO is a very effective and safety method as far as strictly
abide the rules and criterions. The sooner the
management begin, the better the result will be.
刊载交流
Publish Only
K01
Observation of hyperbaric oxygen therapy’s effects
on delayed encephalopathy after acute carbon
monoxide poisoning
Li Li Xu, Yi Liu, Hong Mei Chen
Taihe Hospital Affiliated To Yunyang Medical College,
Hubei, China
Objective: To discuss the effects of hyperbaric
oxygenation in delayed encephalopathy after acute
carbon monoxide poisoning. Methods: 50 patients were
divided randomly into control group (19) with drage
therapy and experimental group(31) with hyperbaric
oxygen therapy based on drage treatment . And then to
evaluate the effects after 1courses, 2courses and
4courses of theatment. Results: The efficacy of
experiment group is obviously difefent (P <0. 05), the
curing time of experiment group was shorter than that of
the control group, the result is signicant different (P <0.
05). Conclutions: Full dosage HBO therapy has obvious
curative effect on delayed encephalopathy after carbon
monoxide poisoning.
K02
The observation of curative and nursing the children
brain damage by Hyperbaric Oxygenation
Dao-dong Liu, Dao-lin Liu
1. The people’s hospital of Lu’an city, Anhui province,
Lu’an, China
2. Luan City Hospital in Anhui Province, Lu’an,China.
Purpose: To observe the curative effect of the hyperbaric
oxygen action used for treating craniocerebral injury of
children and sum up the experience of nursing care.
Method: To retrospect and analyse 160 cases (82
children as an observation group and 78 as a control
group). For the observation group, use the hyperbaric
oxygen action and conventional therapy, at the same time;
exert the holistic nursing of care, such as mental nursing.
At the same time, provide the knowledge about the
hyperbaric oxygen action for the children and the parents
in order to get their coordination. Result: The effective
hyperbaric oxygen action and nursing care made the
curative ratio of the observation group outclass that of the
control group (P<0.005). Conclusion: Treating
craniocerebral injury of children with hyperbaric oxygen
action could get better effect. While at the same time, the
effective nursing care is indispensable.
K03
Brain hippocampus cellular apoptosis and expression
of bcl-2, Bax, caspase3 protein following acute
carbon monoxide poisoning
1
1
2
2
Fujia Liu , Chujin Gao , Chengqing Xia , Hongying Zhao ,
2
Man Qi
1. Hyperbaric Oxygen Department, Beijing Chaoyang
Hospital, Beijing, China
2. Pathological Department, Beijing Chaoyang Hospital,
Beijing, China
Objective: To study the change of apoptotic cells and
expression of bcl-2 protein, Bax protein and caspase3
protein in mice brain hippocampus after acute carbon
monoxide poisoning (ACMP). Mathods: 56 Male
KunMing mice were devided into seven groups (control
group, CO-exposed 1h group, CO-exposed 6h group,
CO-exposed 12h group, CO-exposed 24h group,
CO-exposed 3d group and CO-exposed 7d group), eight
mice each group. The control groups were exposed to air
and the poisoning groups were exposed to CO. General
TUNEL, immunohistochemistry stain and flow cytometry
were used to observe the neurological damage and the
change of caspase3, bcl-2, Bax protein expression.
Results: TUNEL staining positive cells significantly
increased on 3d in hippocampus after acute CO
poisoning(P<0.05), and got much higher value on
7d(P<0.01); caspase3 protein expression increased at 1h
after poisoning, reached the highest at 24h and recovered
on 7d; bcl-2 protein expression increased at 1h in
hippocampus after acute CO poisoning, peaked at 24h
and recovered on 7d; Bax protein expression increased at
1h in hippocampus after poisoning, peaked at 24h and
recovered on 7d. Conclusions: There existed delayed
neurological apoptosis and apoptosis-associated factors
expression in mice brain hippocampus after acute CO
poisoning. Celluar apoptosis might be one of the
pathological mechanism of delayed encephalopathy after
acute carbon monoxide poisoning.
K04
Hyperbaric oxygen therapy to the resumption of
mycoplasma encephalitis patients 1 case
Wencheng Liu, Haiying Jia, Xin Yang, Juan Song
Beijing 306 Hospital of PLA, Beijin, China
Objective: study the role of hyperbaric oxygen therapy to
the resumption of mycoplasma encephalitis patients.
Contents: Female, 5-year-old. She came to hospital
because of fever one day, six hours unclear sense.
Admission diagnosed as mycoplasma encephalitis
merger demyelinating; mycoplasma pneumonia, bacterial
pneumonia, septic shock. She was given such treatment
as
expansion,
down
intracranial
pressure,
anti-mycoplasma, antibiotic, nutritional support to brain
cells, etc. After admission 20 days, pulmonary
inflammation was absorped, and she has a clear
awareness, but not audible, remaining urine incontinence.
Right leg muscle strength Class II, left leg muscle strength
at class I. Review skull MRI: a little bleeding edge of the
bilateral frontal lobe; bilateral subthalamic a small amount
of bleeding. At that time hyperbaric oxygen therapy
started. Methods: GY3200 oxygen chamber of Oxygen
Co. Ltd. in Yantai Hongyuan, the pressure for the
treatment of 2.0 ATA, each 1 days, a total of 30 times.
Results: Ater 10 treatments, coordination of activities was
improved and she,s hands shake reduction. Ater 20
treatments, she can stand and walk. Treatments of 30,
she can speak simple words. Review skull MRI: brain
lesions were disappeared, bleeding was absorped.
Conclusion: Hyperbaric oxygen therapy plays good on
mycoplasma encephalitis. Perhaps we should start a
earlyer application of high-pressure oxygen treatment to
mycoplasma encephalitis patiant just when the disease is
under control.
K05
Interfering effect of hyperbaric oxygen and Ozagrel
Sodium on progressive stroke
Liu Xinying
Jigang General Hospital, Jinan, China
Objective: To approach the therapeutic effect and the
safety of hyperbaric oxygen and Ozagrel Sodium on
progressive ischemic stroke. Methods: Patients with
progressive ischemic stroke were treated with Ozagrel
Sodium in the control group, while in the trial group with
hyperbaric oxygen and Ozagrel Sodium. We judged the
clinical nervous function and defect degree before and
after 7d, 14d, 28d treatment according to the nervous
defect standard (NDS) and the changes of hemorrheology.
Results: There was a significant difference in general
effective rate and significant effectiverate between the trial
group and the control group (P<0.05), and in all laboratory
indexes there was also an apparent effects. Conclusion:
The therapeutic effects of hyperbaric oxygen and Ozagrel
Sodium is satisfactory and safe.
K06
Effects of large doses of hyperbaric oxygen on NF-KB
DNA binding activity of brain tissue on super-early
stage of acute permanent MCAO in rats
Yaling Liu, Qiuhong Yu, Chunjuan Wang, Lianbi Xue
Department of Hyperbaric Oxygenation, Beijing Tiantan
Hospital, Beijing, China
Objective: We investigated the effects of large doses of
hyperbaric oxygen (HBO) on acute permanent focal
cerebral ischemia in rats. And we examined if hyperbaric
oxygen treatment (HBOT) could influence NF-KB DNA
binding activity of peri-infarction region of brain tissue.
Methods: Seventy rats underwent MCAO operation and
were randomized to hyperbaric oxygen treatment group,
control group, sham group. Hyperbaric oxygen treatment
began 5h after surgery. HBOT was delivered at a dose of
two atmospheres absolute (ATA) for 1 hour ten times four
day. Control animals were exposed to air at ambient room
pressure.
Other
rats
underwent
sham
surgery. Neurological outcome was measured 3h, 24h, 2d,
3d, 5d after surgery using Garcia scores. TTC
pathological staining and Image J software were used to
determine infarction volume at the point of 3h, 24h, and 5d
after surgery. And we investigated NF-KB DNA binding
activity of brain tissue 3h, 24h, 5d after surgery using
electrophoretic mobility shift assays (EMSA). Results:
(1)Garcia scores of HBO group(3.64±0.18) at the point of
24h after MCAO were significantly higher than control
group (3.21±0.12) (P<0.05). There was no significant
difference in Garcia scores between HBO group and
control group 3h and 5d after MCAO. (2) At 3h after
cerebral ischemia there was no infarction region. There
was no significant difference in infarction volume between
HBO group and control group at 24h after cerebal
ischemia. From 24h to 5d after MCAO HBO group
infarction volume didn’t change, but that of control group
decreased. (3)NF-KB DNA binding activity in HBO group
(1.66±1.15) at 5d after MCAO was significant lower than
control group (7.28±3.10) (P<0.05). But at 3h, 24h after
MCAO there was no significant difference in NF-KB
activity between two groups. And there was no significant
difference in NF-KB DNA binding activity between
ischemic ipsilateral cortex and contralateral cortex.
Conclusions: (1) Hyperbaric oxygen could protect
ischemic brain tissue at 24h after MCAO surgery, but after
24h HBOT should be used carefully ,because it might
damage brain tissue. (2) Hyperbaric oxygen could
decrease NF-KB DNA binding activity of ischemic
ipsilateral cortex.
K07
Fracture treatment by hyperbaric oxygenation
Yi Liu, Cao Cui
Taihe Hospital Affiliated to Yunyang Medical College,
Hubei, China
Objective:
To investigate the curative effect of
hyperbaric oxygenation in patients with recent fracture.
Methods:
80
cases
were
divided
into
hyperbaric-oxygenation group and the control group; in
the hyperbaric oxygenation group, under 0.2 MPa
pressure, patients inhaled pure oxygen for 60 minutes
each day, and between10 times and 60 times. Results:
there was great difference between two groups(P<0.05).
Conclusion: Hyperbaric oxygenation can promote the
healing of fracture.
K08
Effects of supplementing Qi and activating blood
circulation and hyperbaric oxygenation on pia mater
microcirculation in gerbil after cerebral ischemic
reperfusion injury
Guoping Yu, Shilong Lin, Min Fang
Shangahi Kaiyuan Orthopaedics Hospital, Shanghai,
China,
Objective: To explore the efficacy of Chinese drugs with
the function of supplementing Qi and activating blood
circulation and hyperbaric oxygen (HBO) on the pia mater
microcirculation in gerbil after cerebral ischemia /
reperfusion injury. Methods: Forty － four Mongolian
gerbils underwent following operations: A cranial window
was created in the left parietal bone and covered with
absorbent cotton soaked with artificial cerebro-soinal fluid.
The bilateral common carotid arteries were squeezed by
clamps for 30 minutes and then the clamps were
loosened so as to cause reperfusion．The gerbils were
equally divided into four group at random: drug group
(compound solution including Astragalus root, Chinese
angelica, and red sage root was infused intraperitoneally),
HBO group (exposed to 0.25MPa hyperbaric oxygen for
60 min) ,combined treatment group (both the two above
methods were used) ,and control group (normal saline
was
infused
intraperitoneally),
LMB-1
laser
microcirculatory mater and JI-200 laser microcirculatory
moving analytical meter were used to observe the
changes of pia mater microcirculation through the cranial
windows before cerebral ischemia. Results: After the
common carotid arteries were squeezed, the
microvessels contracted obviously, microthrombus were
seen, and blood flow rate of the arteriole decreased to
0.45mm/s, and the blood flow rate of the venule
decreased to 0.38mm/s.It was observed that in the drup
group, HBO group, and combined treatment group the
microthrombi in pai mater were loosened gradually, and
exudation around microvessel was gradually entered
absorption up to extinction. The arteriole blood flow rate
was 1.46-1.56mm/s, the venule blood flow rate was
1.27-1.44mm/s,significantly higher than those of the
traditional. Conclusion: Chinese drugs with the function
of supplementing Qi and activating blood circulation by
playing a role in improving microcirculation. Their effects
are closely related to acceleration of the blood rate,
regulation of the caliber of the arterioles and the venules,
and improvement of cerebral blood flow except oxygen
content increased.
K09
The effects of hyperbaric oxygenation on the
treatment of spinal cord injury
Zhicai Li, Yiping Song, Xiaoping Mu
No. 97 hospital, Xuzhou, Jiangsu, China
Aim: To observe the effects of hyperbaric oxygenation on
the function restoration in patients with spinal cord injury.
Methods: All patients with spinal cord injury were
confirmed with CT and/or MRI. 126 patients enrolled after
January 1986 were assigned to treatment group (T group,
with hyperbaric oxygenation therapy) and 56 patients
enrolled before December 1986 were assigned to Control
group (C group, without hyperbaric oxygenation therapy).
There was no significant difference between two groups
on the gender, age, course and injured position. Patients
in two groups were operated and administrated with
dehydrant, anti-inflammation, and drugs to against the
oxygen free radicals. The puncture and other physical
treatments were given to all patients, but only the patients
in T group were treated with hyperbaric oxygenation. The
hyperbaric oxygen chamber was pressurized to 0.2Mpa
and maintain at this level. The patients in T group were
given oxygen with mask according to the protocol
schedule of 30min×2+10min, and total time was 120min.
A patient would need 2-4 periods of treatment dependent
on the severity of injury and 10 times as one period of
treatment. The relationship between efficiency and the
beginning time of hyperbaric oxygenation therapy was
observed. Results: The rate of efficiency and
improvement was 61.9% and 31.0% respectively in T
group, which was significantly more than 23.2% and
37.5% in C group (p<0.05). Conclusion: Hyperbaric
oxygenation could promote the function restoration of
injured spinal cord, especially for the patents with partly
spinal cord injury. The patients with spinal cord injury
should be treated with hyperbaric oxygenation as earlier
as possible.
K10
Cases reports for conventional hyperbaric oxygen
treatment on altitude decompression sickness
Guangsen LIU, Shuhui ZHANG
The Centre of HBOT in 458th Hospital of PLA,
Guangzhou, China
Objective: The purpose of this study was to evaluate
clinical effects of conventional hyperbaric oxygen
treatment on 17 cases with altitude decompression
sickness (DCI). Methods: 17 male patients (age: 25-45yr)
with altitude decompression sickness were received by
conventional HBO in single chamber filled with pure
oxygen. The high altitude presented DCI occurred from
7500m to 14000m because of chamber windows breaking
abruptly. The study population consisted of experienced
pilots with the flight time from 400 to 4500 hrs. All of them
had different symptoms respectively, i.e. memory loss,
hearing loss, confusion, blurry vision, headache, fatigue,
joint ache. Some symptoms lasted 3-23 days after landing.
All of them had received 1-3 sessions HBO (0.295MPa,
90min, one session per day) in first 3 days, and then 1
session HBO (0.20 MPa, 80min). Results: All symptoms
of them were completely relieved after 1-3 sessions with
HBO. The all examinations for the study population were
normal as before after 20 sessions HBO. 16 of 17 pilots,
except 1, continued high altitude flight as before.
Conclusion: In this study population, symptoms with
altitude DCI would be relieved by conventional HBO.
K11
Curative effect of hyperbaric oxygenation
peripheral neuropathy in type II diabetics
Jiaxiang Bai, Yanxin Liu
Tianjin Hospital, Tianjin, China
on
Objective: To observe the curative effect of hyperbaric
oxygenation (HBO) on peripheral neuropathy in type II
diabetics. Methods: 76 diabetics were divided into 2
groups equally. Diabetic diet, hypoglycemic agents,
vitamin B1 20mgtid Oral treatments were used in control
group. HBO combined with the above treatments were
used in HBO group. Patients stayed in 2.5 ATA HBO
chamber, wearing masks and inhaling pure oxygen for 20
min 4 (of pure oxygen absorption 80 minutes), with 5 min
interval. Once a day for 10 times as a course, altogether 4
courses for each patient, breaks 3-5 days.
Electromyography (EMG) was measured before treatment
and two months after the HBO treatment done. Results:
In HBO group, the clinical symptoms improved
significantly or disappear, the motor nerve conduction
velocity (MNCV) and sensory nerve conduction velocity
(SNCV) of EMG were increased significantly after the
treatment than before (P < 0.01), while clinical symptoms
of the control group had no significant improvement, in
EMG Figure part (upper) a slight improvement in nerve
conduction velocity, and no other significant changes.
Conclusion: HBO can effectively improve diabetic
peripheral neuropathy, worthy of promotion.
were significantly higher than in control group (P<0.05).
Conclusion: HBO may help to recover injured patients’
language abilities.
K12
Effect
of hyperbaric oxygen treatment
on
hydrocephalus in animal with acute ischemic cerebral
injury
1
2
2
1
Shilong Lin , Jingchang Liu , Peizhu Xin , Guooping Yu
1.Shanghai Kaiyuan Orthopedic Hospital , Shanghai,
China
2.Naval Medical Research Institute of PLA, Shanghai,
China
Objective: To approach effect of hyperbaric oxygen
(HBO) treatment on hydrocephalus in animals with acute
cerebral ischemic injury. Methods: 60 gerbils were
divided into the cerebral ischemic group, the hyperbaric
oxygen exposure group and the control group. These
animals were anesthesia with 20% urethane (1.2g/kg.BW)
or 20% pentobarbital sodium (40mg/kg B.W.). Acute
cerebral ischemic injury was resulted from occluding both
carotid for 30min. Animals were exposed at 0.25MPa
HBO for 60min after cerebral ischemic injury in the HBO
group. Cerebral ischemic injury group was exposed to
N2-O2 with O28%-N292% at 0.25MPa for 60min. The
control group animals were exposed to normal pressure
for 60min in the chamber. Blood flow dynamic effect in
microcirculation, morphosis in micro-vascular endothelial
cell and cerebral nerve cell detected. Result: after 30min
to block up carotid, cerebral nerve cell injury occurred,
part nerve cell nucleus was vacuole, denaturalization and
swell. Cerebral tissue was edema. Endothelial cell
structure and function changed, and edema formed
around micro-vessel. After HBO exposure for 60min,
cerebral nerve cell swell improved in animal with cerebral
injury. Only spot exudation occurred around micro-vessel.
Endothelial
cell
injury
obviously
decreased.
Hydrocephalus entered absorption. After HBO exposure
at 0.25MPa for 60min, blood perfusion flow increased
than the control group in animals with cerebral ischemic
injury (P<0.01). Conclusion (1) Acute ischemic cerebral
injury may cause hydrocephalus resulted in cerebral
nerve cell, endothelial cell and other cerebral tissues
injury. (2) HBO has therapeutical effect by reducing injury
in nerve cell and micro-vessels endothelial cell in animals.
K13
Interfering effect of hyperbaric oxygen on alogia in
acute cerebral injury
Lin Ying
Hyperbaric Oxygen Derpartment of the Affiliated Zhong
Shan Hospital of Xiamen University, Xiamen, China
Objective: To study the interfering effect of hyperbaric
oxygen on aphasia people with acute cerebral injury.
Methods: 120 cerebral injury people were divided into the
hyperbaric oxygen (HBO) exposure group and the collator
group. Each group had 60 people. HBO group were
accepted HBO treatment. Results: After 12-24 times or
more HBO treatment, it showed that the blocked vessels
were repassed or injured cerebral zones were rebuilt from
CT or MRI impression. Injured patients’ language abilities
K14
Hyperbaric oxygen treatment of 28 cases of children
viral encephalitis
Xiuqing Liang, Xiaole Wang, Xiaohong Wang
Shanxi Province Maternal and Children Care Hospital,
Xi’an, China
Viral encephalitis in pediatric clinical is common, and
major features of fever, headache, convulsions,
disturbance of consciousness, abnormal behavior, high
intracranial pressure. Laboratory abnormalities can be
found in cerebrospinal fluid, the virus culture positive,
cranial CT and EEG abnormalities, etc. In 1991 to 2007,
admitted in October to 11-year-old children with 28 cases
of encephalopathy with anticonvulsant, decrease
intracranial pressure and the anti-virus treatment,
especially add to hyperbaric oxygen therapy. All of the
patients were given oxygen chamber treatment. 0.08 Mpa
pressure lasts two hours, once a day for 10 consecutive
treatment every week treatment interval. course of
treatment is from 2 to 8. The results showed that cured 16
cases, eliminate clinical symptoms, EEG, cerebrospinal
fluid and cranial CT returned to normal. 9 cases turn for
the better, eliminate clinical symptoms, cerebrospinal fluid
of normal, but abnormal EEG and cranial CT abnormal;
invalid three cases, No clinical symptoms improve,
continuing convulsions, abnormal EEG and cranial CT,
brain conversion is shown. Hyperbaric Oxygen Therapy
encephalopathy is mainly to facilitate the brain tissue
collateral circulation, reducing hypoxic brain damage,
increasing reticular activating system’s oxygen, to speed
up the unconscious child awake. Compared with simple
dehydration, anti-virus and anticonvulsant treatment,
hyperbaric oxygen is significantly. In addition, we also
found that the severe convulsions, plus saiga horn oral
solution, which can effectively reduce the seizures
occurred and prevent temperature rebound.
K15
Study on prevention of the delayed neuropsychologic
sequelae after acute carbon monoxide poisoning in
hyperbaric oxygen treatment
Li Zengmin
Hyperbaric oxygen section of City center hospital, Xian,
China
Objective: Research how lower the delayed
neuropsychologic sequelae of outbreak rate in the
hyperbaric oxygen treatment the acute the carbon
monoxide poisoning of process. Methods: Through
review cultural heritage and r former clinical data of
analysis, take a series valid measure, to hyperbaric
oxygen treatment the project did correspond of
improvement. Results: Make acute carbon monoxide
poisoning the delayed neuropsychologic sequelae of
outbreak rate reduced obviously. Conclusion: Combine
immediate, enough of hyperbaric oxygen treatment and
reasonable of comprehensive treatment are the best
treatment project.
K16
Purpose judged curative effect of wean cerebral palsy
by hyperbaric oxygen treatment.
Zuomin Li, Wenying Huang
First Renmin Hospital of Chenzhou, Huhan, China
Purpose: Judged curative effect of wean cerebral palsy
by hyperbaric oxygen treatment. Method: 75 case
cerebral palsys were divided into two groups: treatment
group 38 case and antitheses 37 case. Compared the two
groups with symptom, system by crued fore-and-aft; and
compared the conclusion based on CT or MIR result.
Result:
Myospasm,
involuntary
movements,
incoordination, intellectuality, speech function improved in;
epilepsy spasm `times decreased; it is more difference
compare with antitheses of HBOT group’s case (p<0.05).
Conclusion: HBOT is credibility and affirm method than
simplicity medication in wean cerebral palsy treatment.
K17
Monitoring bacteria status in the water and container
of humidifying in single-chamber with pure oxygen
Chaoqun TU, Xiaomei XIE, Xiufang MEI, Meilian LAN, Sh
uliang LI
The Shenzhen Peoples Hospital, Shenzhen, China
Objective: To observe the bacteria status in the water
and container of humidifying in the single-chamber with
pure oxygen in order to find right method to prevent from
second contamination from humidifying water in the
chamber. Methods: 1.The water in the container of
chamber and from spraying nozzle in the chamber was
cultured for consecutive 5 days. The water was divided
into three groups, non-heating water, drinking water and
distilled waterdistilled water, was changed on alternate 3
3
days145±26 cfu/m )found at the two sites from
non-heating water appeared on third days. The TBC from
drinking water and distilled water were respectively
3
3
472±69 cfu/m , 357±91cfu/m on forth days. 2. No bacteria
appeared at the above sites from the water of drinking and
distilled water on the third day when water had been
changed on alternate 3 days. Conclusion: 1.The distilled
water should be best liquid fit to humidify in the
single-chamber with pure oxygen. 2. It should be to clean
the container and distilled water should be changed at
least on alternate days . . and was cultured at the same
method on third day. Results: 1. No bacteria of three kind
of water were found at the two sites. The bacteria
(average total bacterial count, TBC. 2. The water divided
into two groups, drinking water and
K18
The affection research of micronucleus frequency in
pregnant mice bone marrow erythrocyte treated by
hyperbaric oxygen
Li Zhang
Second hospital attached to Kunming medical collage,
Kunming, China
Objective: This paper has evaluated the frequency of
micronucleus in the pregnant mice bone marrow
erythrocyte treated by hyperbaric oxygen. Methods: We
have treated the pregnant mice by hyperbaric oxygen one
time every day, and examined the frequency of
micronucleus in the pregnant mice bone marrow
erythrocyte, in order to research the affection of the
genetic materials in pregnant mice treated by hyperbaric
oxygen. Results: The frequency of micronucleus in the
treated group is significant different compared with the
control group. Conclusion: Perhaps excessive
hyperbaric oxygen can make damage of the genetic
material in the pregnant mice to a certain degree.
K19
The penis plants again match carried on the
hyperbaric oxygenation treatment to cure 1
Li Zengmin
Central Hospital XIAN, Xi’an, China
Objective: The penis plants again behind for the sake of
anti- infect and improve the blood oxygen a supply ,the
match carried on the hyperbaric oxygenation treatment.
Methods: The treatment adopts large air to add to press
cabin ;normal regulations treatment project. Results and
Conclusion: Thus for again the surgical operation
provided a guarantee successfully.
K20
Effect of Hyperbaric Oxygen Treatment on learning
and memory aspects of transient global cerebral
ischemia in rats
1
1
2
1
Yajuan Li , Chunjin Gao , Yu Zeng , Zhipeng Xu , Hui W
1
ang
1. Capital Medical University affiliated Beijing Chaoyang
Hospital, Beijing, China
2. Sichuan Provincial Peoples Hospital, Chengdu, China
Objective: To observe the effect of hyperbaric oxygen
treatment (HBOT) on rat’s abilities of leaning and memory
in the transient global cerebral ischemic model and to
explore the mechanism of hyperbaric oxygen treatment
on recognition impairment after cerebral ischemic
diseases. Methods: 46 male Sprague-Dawley rats were
randomly divided into 4 different groups: blank group
(n=10), sham-operation group (n=10), cerebral-ischemia
group (n=9), HBO group (n=9). According to Pulsinelli’s
4-vessel occlusion method to establish transient global
cerebral ischemic model on the cerebral ischemia and
HBO group. The sham-operation group didn’t block the
vertebral artery and the carotid artery. The blank group
has no operation, only take part in Morris water-maze.
HBOT was performed to rats in HBO group after the
second operation for 7days, one time for one day, then we
give all the rats a test of Morris water-maze. We observed
the time of escape latent and the last day when move off
the platform, the time of rats through the platform .The
average time of every day and every group was compared.
The SPSS11.5 software was used for statistical analysis,
st th
rank sum test was used in the 1 -5 ,analysis of variance
and Newman-Keuls test were used in the last day, p<0.05
has statistical significance. Results: 1. The escape latent
comparison:the HBO group is shorter than the cerebral
ischemia in the 3rd -5th day (p<0.01 or p<0.05), the
difference has statistical significance; the HBO group and
sham operated group in the 1st -5th day has no difference;
the sham operated group is shorter than the cerebral
ischemia group in the 3rd and 5th days (p<0.01 or p<0.05),
the difference has statistical significance; the blank group
is shorter than the sham operated group in the 2nd- 5th
day (p<0.01 or p<0.05), the difference has statistical
significance.2. The times of through platform's
comparison: the HBO group is larger than the cerebral
ischemia group in the last day (p<0.05), the difference has
statistical significance; the HBO group and the sham
operated group has no difference; the sham operated
group is larger than the cerebral ischemia group(p<0.01),
the difference has statistical significance; the blank group
is larger than the sham operated group (p<0.01), the
difference has statistical significance. Conclusions: 1.
The learning and memory abilities of transient global
cerebral ischemia in rats are decline. 2. HBOT can
improve the impairment of learning and memory after
transient global cerebral ischemia in rats.
K21
Effect of High pressure oxygen (HBO) for Treating
Neonates with Bypoxic ischemic encephal opa-thy of
the newbom (HIE)
Yuxian Li, Mei Lin
Youjiang Medical College for Nationalities of Affiliated
Hospital, Guangxi, China
Objective: To study the effect of HBO for treating
neonates with HIE. Methods: 160 cases hospitalized
in our department who were diagnosed with HIE
according to the diagnosis stander. They wear divided
into treatment group and control group. There was no
significant difference between the two groups of the
clinical classification, lesion degree and days (p>0.05).
Control group was given regular treatment, while
treatment group was added HBO after the life sings
was stable. The treating pressure adjusted to the days
old.10 days were a course .They were assessed after
the treatment .The results were compared. Results:
There was difference between the two groups of the
effective rate. Effect of treatment group was better than
that of control group. All cases were followed up for
half a year. Their neural development and the
intelligence were observed .There was statistic
difference between two groups adverise prognosis rate.
Conclusion: patients with HIE should be given HBO
as soon as possible after stable disease .It can
improve the clinical symptom and the cure rate
shorten ,the course of disease.
K22
The hyperbaric oxygen therapy for spinal cord injury
caused by acute cervical disc herniation
Shaohua Liang, Xiaoju Tang, Ruzhuan Liu
The Ruikang Hospital Affiliated to Guangxi University of
Traditional Chinese Medicine, Nanning, China
Objective: To evaluate the clinical effects of the
hyperbaric oxygen therapy for spinal cord injury caused
by acute cervical disc herniation. Methods: To
retrospectively analysize the clinical data of spinal cord
injury caused by acute cervical disc herniation of 45
cases ,which was treated with the hyperbaric oxygen
therapy after surgery. To evaluate the clinical effects,
based on the Frankel grade and the JOA score
improvement rate of preoperative and postoperative
situation. Result: the Frankel grade of patients improves
1-4 level. The time of follow-up is from 6 months to 2
years, the average time is 10 months. the JOA score
improvement rate: excellent: 23 cases(51.1%), good: 18
cases(40.0%), average:3 cases(6.7%), poor:1 case (2%),
The good-to-excellent rate is 91.1%. Conclusion: The
hyperbaric oxygen therapy for spinal cord injury caused
by acute cervical disc herniation, is the effective therapy
of recovery of spinal function.
K23
Clinical studies on sudden deafness via hyperbaric
oxygen therapy
1
2
Ying Mei , Chaofeng Xie
1. HBO Department, People’s Hospital of Guangdong
Province, Guangzhou, China
2. E.N.T Department, Shantou Second People’s Hospital,
Shantou, China
The therapeutic effect on the clinical efficacy of hyperbaric
oxygen treatment on sudden deafness was discussed in
this study. The method is that 300 cases of sudden deaf
patients were randomly divided into two groups: drug
treatment group (group A),and the hyperbaric oxygen
treatment with drug group(HBO group, or group B). the
two groups with drug treatment made the same effect, but
in addition, HBO group was given the treatment of HBO,
that is, under the following conditions: the
pressure-0.22Mpa,oxygen
absorption-30min*2,the
interval-5min,one treatment for one day,12 days for a
course. Generally, the effect comparisons were taken in
one to three courses. Overall, the effect of the
combinative group is better than the drug group, and the
variation is obvious (P<0.05). And the conclusion is: the
treatment on sudden deafness with hyperbaric oxygen
and drug is obvious, and should be advocated broadly in
the clinical application. Keywords: sudden deafness
hyperbaric oxygen combination treatment
K24
To treat hard facial nerve paralysis with hyper--baric
oxygen (HBO)combined with electric acupuncture
Shunyong Li, Aixiang Du, Guicui Wang
WeiHai Municipal Hospital, Weihai, China
Objective: To compare the curative effect of HBO in
patients with hard facial nerve paralysis with of HBO
combined with electric puncture. Methods: 37 cases of
patients were treated simply with HBO once per day,the
duration of one period of treatment lasted for 10 days.
39cases of patients were treated with HBO and electric
acupuncture for five period of treatment (To acupuncture
the routine facial nerve paralysis points once per day, ten
times of acupuncture made up of one period of treatment).
Results: Of the HBO group, 12 cases (32.43%) were
cured, 18cases (48.65%) were relieved, 7cases (18.92%)
were no curative effect. Of the HBO combined with
electric acupuncture group, 24 cases (61.54%) were
cured, 12 cases (30.77%) were relieved, 3cases (7.69%)
were no curative effect. Conclusion: The curative effect
of using HBO combined with electric acupuncture in
treating patients with hard facial nerve paralysis is
significantly better than that of using HBO simply.
K25
Effect of early intervention of hyperbaric oxygen and
hypobaria on CGRP and ET level in rats under
hypobaric environment
1
1
1
Shuyi Pan , Xiaowen Pan , Xiangen Meng ,
1
1
1
2
Mingxin Li , Yu Zhang , Chen Yang , Baoguo Li
1.Navy General Hospital, Beijing, China
2.Shi jitan Hospital, Beijing, China
Objective: To explore the effect of early intervention of
hyperbaric oxygen and low atmospheric pressure on
CGRP and ET level under hypobaric environment, and to
compare the effects of two methods. Methods: The
Wistar rats were randomly divided into 6 groups: (1)
Control group, (2) 3800m (2h) group, (3) 3800m (3d)
group, (4) 3800m (7d) group, (5) Hyperbaric oxygen
intervention
group,
(6)
Hypobaric
environment
intervention group. Firstly, the rats in the second, third and
fourth groups were placed in low pressure oxygen
chamber, and the pressure in chamber was decreased at
equal speed from 0.1mpa to 0.062mpa from the sea level,
to simulate hypobaric environment on mountain at 3800m
above sea level. At 2h, 3d and 7d time points, the rats
were decapitated to obtain blood for later examination;
Secondly, the rats in the fifth group received hyperbaric
oxygen treatment (0.2mpa, once per day, oxygen
inhalation for 60 minutes, successively 5 days). At the
sixth day, the rats were placed in low pressure oxygen
chamber, and the chamber was decreased at equal speed
to 0.062mpa. At 2h time point, the rats were decapitated
to obtain blood for later examination; thirdly, the rats in the
sixth group received hypobaric hypoxia treatment
(0.062mpa, once per day, 2h once, for successively 5
days). At the sixth day, the rats were placed in low
pressure oxygen chamber, and the chamber was
decreased at equal speed to 0.062mpa. At 2h time point,
the rats were decapitated to obtain blood for later
examination; the blood samples obtained from above
different treatment methods were centrifugated to obtain
the supernatant, and CGRP and ET levels were
measured by radioimmunology method. Results:
Compared with control group, CGRP level shows
decreasing tendency with time extension, without
significant difference. The hyperbaric oxygen treatment
has no effect on CGRP level, but hypobaric hypoxia
pretreatment can make CGRP level decrease more
obviously, without significant difference. ET level shows
increasing tendency with time extension, but without
significant difference. However, EF level after hyperbaric
oxygen and hypobaric hypoxia pretreatment shows
significant increasing, P<0.05. Conclusion: The
hyperbaric oxygen and hypobaric hypoxia training mainly
influences ET level change in rats, and hyperbaric
pretreatment might enhance body adaptive capacity on
mountain.
K26
Observation of hyperbaric oxygenation theraphy on
patients with insomnia
Yulin Nie, Kunru Zhou
Buji people’s hospital, Shenzhen, China
0bjective: To observe the effects of hyperbaric
oxygenation on sleep quality of the patients with
insomnia. Methods: Ninteen patients with insomnia were
treated with hyperbaric oxygenation, and observe the
effects of hyperbaric oxygenation on sleep quality and
simultaneous phenomenon. Results: 5 cases totally
recovered, 12 cases had obvious effect, and 2 cases had
no effect. Conclusion: Sleep quality and simultaneous
phenomenon of the patients with insomnia can be
improved with hyperbaric oxygenation.
K27
Use hyperbaric oxygen therapy to treat radiating
injury
Jun Qu, Jun Meng
HuanHu Hospital, Tianjin, China
We all know that radiating injury often can be found in
radiation therapy of malignancy tumour. Rradiating injury
has a strong impact on patient’s life quality. Hhyperbaric
oxygen therapy (HBOT) which known as a useful way to
some disease had application in curing the patients. But
there were few reports about radiating injury now. This
review was discuss about the mechanism of how radiating
injury happened and the mechanism of HBOT to treated it.
HBOT also can be consider as a new way to cure
radiating injury.
K28
The diagnosis and treatment experience of hyperbaric
oxygen (HBO)
comprehensive treatment
for
persistent vegetative state (pvs)
Juan Meng, Xiaowen Pan, Yu Zhang, Huijun Hu, Yan Lu,
Shuyi Pan, Xiangen Meng
Hyperbaric oxygen center in the General Naval Hospital,
Beijing, China
Objective: to discuss and study the treatment effect of
hyperbaric oxygen (HBO)comprehensive treatment for
persistent vegetative state(pvs) and the importance of
advanced radiological technology for pvs. Methods: for
280 pvs patients with HBO cooperating with blood
magnetization therapy and drugs treatment and with
advanced radiological technology. Results: this group of
patients showed that the diagnosis coincidence rate
reached 100%, the curative ratio (85 cases of clear
consciousness, normal intelligence and self-care of living)
is 30.4%, the improvement rate (48 cases of clear
consciousness, basal self-care of living and basal normal
intelligence) is 52.8%, 15.2% (43 cases) of patients have
not left PVS, 98.4% of patients have gained improvement
of general condition (276 cases of control of infection,
correction of complication, pulling out of urethral catheter,
nasal feeding tube and tracheal tube after tracheotomy
and improvement of nutrition status), 1.6% of patients (4
cases) died. The major abnormal signs in cerebral CT of
this 280 cases PVS patients contained: cerebral edema,
malacia focus, focus of infarct, subdural effusion,
obstructive hydrocephalus, bilateral hypophloeodal diffuse
white matter demyelination change, bilateral cerebrum
atrophy, thinner cortex, ventricles of brain enlarging,
broaden of sulcus and schizencephaly, malacia focus of
basal nuclei and globus pallidus in the damage region. 76
cases of MRI examination could also show the atrophy of
patients’ mesencephalon and brain stem except for the
signs before. Cerebral SPECT: 32 cases showed
decreasing of bloodstream perfusion in bilateral or
unilateral cerebral cortex, cerebellum, unilateral or
bilateral frontal lobe, temporal lobe and occipital lobe. The
radiological imaging of mostly patients improved along
with improvement of state of illness. Conclusion: the
HBO comprehensive treatment scheme was reasonable,
advanced, practical and innovative. The curative effect
was good: Management to complications was timely and
effective. Advanced radiological technology cerebral CT
and MRI are not only helpful to diagnosis, but also helpful
to guide treatment and judge prognosis. The cerebral
SPECT imaging could be complementary with CT and
MRI. It also could act as an index of HBO comprehensive
treatment for PVS and prognosis determination.
K29
Therapeutic efficacy of hyperbaric oxygen on middle
Jiexiang Pan, Weihua Chu, Fei Li, Hua Feng
Department of Neurosurgery, Southwest Hospital, Third
Military Medical University, Chengdu, China
Objective: To evaluate the efficacy of hyperbaric oxygen
(HBO) treatment on model of right middle cerebral artery
occlusion (MCAO) in rabbits. Methods: Forty healthy
rabbits were suffered MCAO using electragulation and
were randomly divided into 4 groups. The rabbits in I
group were treated with HBO immediately for 20 days
after MCAO. The rabbits in D group were treated with
HBO 7 days after MCAO for 13 days. The rabbits in C
group were not treated with any therapy after MCAO. The
methods of S group were similar to that of C group except
no MCAO. The change of behavior, somatosensory
evoked potential (SEP) and infarct volume in these rabbits
was observed respectively for 10 days and 20 days after
MCAO. Results: Ten days after MCAO, the mean score
of behavior of I, D, C and S groups was respectively
8.6±1.2,
5.8±0.8,
5.6±1.6
and
12±0.0.
The
neurobehavioral impairment in I group relieved
3
significantly compared with that of D and C groups, (p ,
3
3
3
214±19.7 mm , 229.4±25.5 mm and 0 mm for 10 days
after MCAO, the infarct volume of I group was significantly
3
3
smaller than that of D and C groups (p , 203.4±12.7mm ,
3
3
221.4±20.3mm and 0mm .The infarct volume of I group
was significantly smaller than that of D and C groups
(p<0.01). Conclusion: Early applications of HBO and
repeated administration of HBO for 20 days have
significant effects on the change of behavior,
somatosensory evoked potential (SEP) and infarct volume
of rabbits which suffering from MCAO.
K30
The therapeutic efficacy of hyperbaric oxygen
treatment for brain infraction was influenced by the
onset time: micro dialysis study in rabbits
Zhaopang Lai, Fei Li, Zhi Liu, Hua Feng
Department of Neurosurgery, Southwest Hospital, Third
Military Medical University, Chengdu, China
Objectives: To observe if the therapeutic efficacy of
hyperbaric oxygen treatment for brain infraction was
influenced by the onset time after permanent middle
cerebral artery occlusion (MCAO) in rabbits. Methods: 36
male rabbits were randomly divided into simple MCAO
group (n=12) , MCAO+ HBO group (100% O2, 2.5 ATA,
1h / d, from 1 d after MCAO, n=12) and MCAO＋DHBO
group (100% O2, 2.5 ATA, 1h / d, from 7 d after MCAO,
n=12). Behaviors and volumes of infarction were
observed, microdialysis was applied to monitor the
concentrations of glucose, lactate, pyruvate and
glutamate around the infracted zone at 1,3 ,10and 30 d
after permanent MCAO. Results: Behaviors’ score and
volumes of infarction were lower in MCAO+ HBO group
than the others (P<0.01). The lactate and pyruvate ratio
increased after MCAO in three groups, but they were
lower in the MCAO+ HBO group than in the others at Day
1 and Day 3(P<0.05). The glutamate concentration
increased after MCAO, peaking at 10 d, but at day 1 and
day 3 the glutamate concentration was lower in the
MCAO+ HBO group (P<0.05). Conclusions: Hyperbaric
oxygen treatment could protect the brain from infarction
through improving the energy metabolism and decreasing
the excitatory amino acids disorders around the infracted
zone after MCAO in rabbits. In order to improve the
therapeutic efficacy of hyperbaric oxygen, it should be
performed as early as possible.
K31
The expression of nestin was elevated by hyperbaric
oxygen treatment in a model of right middle cerebral
artery occlusion in rabbits
Jiexiang Pan, Weihua Chu, Fei Li, Hua Feng
Department of Neurosurgery, Southwest Hospital, Third
Millitary Medical University, Chengdu, China
Objectives: To evaluate the efficacy of hyperbaric
oxygen (HBO) treatment on the expression of Nestin in a
model of right middle cerebral artery occlusion (MCAO) in
rabbits. Methods: 30 healthy rabbits were randomly
divided into 3 groups: The rabbits in MCAO+HBO group
were treated with HBO immediately for 20 days after
MCAO, The rabbits in MCAO group were not treated with
any therapy after MCAO, The methods of control was
similar to that of MCAO group except no MCAO.
Behaviors and volumes of infarction were observed, and
the expression of Nestin was measure by
immunocytochemistry at 10 and 20 days after MCAO.
Results: Behaviors’ score and volumes of infarction were
better in MCAO+ HBO group than the MCAO group
(P<0.01).10 days after MCAO, the expression of Nestin of
MCAO+ HBO group and MCAO group was respectively
15.88±1.2 and 6.63±1.6. 20 days after MCAO, the
expression of Nestin of MCAO+ HBO group and MCAO
group was respectively 20.03±1.6 and 6.82±0.8. The
expression of Nestin in MCAO+HBO group relieved
significantly higher compared with that of MCAO group
(p<0.01). Conclusion: Early applications of HBO and
repeated administration of HBO for 20 days have
significant effects on the expression of Nestin of rabbits
which suffering from MCAO.
K32
Regional biochemical changes following ischemic
brain injury in rabbits ： microdialysis studies and
effects of hyperbaric oxygen preconditioning
Chongying Deng, Fei Li, Lijun Zhang, Hui Meng, Jiangkai
Lin, Hua Feng
Department of Neurosurgery, Southwest Hospital, Third
Military Medical University, Chengdu, China
Objectives: To observe the variations of regional
biochemical changes around the infracted zone after
permanent middle cerebral artery occlusion (MCAO) in
rabbits and evaluate the effects of hyperbaric oxygen
precongitioning (HBOP). Methods: 48 male rabbits were
randomly divided into simple MCAO group (n=24) and
HBOP+MCAO group (100% O2, 2.5 ATA, 1h / d, 5d,
n=24) . Microdialysis was applied to monitor the
concentrations of glucose, lactate, pyruvate and
glutamate around the infracted zone at 1, 3, 10 and 20 d
after permanent MCAO. Results: Glucose decreased at 1
d after MCAO in two groups, but in the preconditioning
group glucose recovered to normal at 3 d, while in the
simple MCAO group it still decreased at 20 d and
significantly lower than the left (PP<0.05). Conclusions:
1, The variations of the components of the microdialysis
interstitial fluid from the brain support the presence of
“biochemical penumbra” around the infracted zone after
MCAO；2, the mechanisms of HBOP maybe improving the
energy metabolism and decreasing the excitatory amino
acids disorders around the infracted zone after MCAO in
rabbits .
K33
Use hypenbaric oxygen( HBO) to treat 8 patients of
viral meningitis
Jun Meng, Jun Qu
HuanHu Hospital, Tianjin, China
Objective: Use hyperbaric oxygen (HBO) to treat viral
meningitis. Method: Use hyperbaric oxygen (HBO) to
treat 7 patients of viral meningitis and we will observe the
change of hydrocephalus in medical image and the
recovery of consciousness, language, limb strength.
Result: All the patients have a good curative effect in
what sides we observed. Conclusion: Use hyperbaric
oxygen therapy to treat viral meningitis at the end of the
course maybe beneficial to the recovery of the naval
function.
K34
Effect Analysis of early and delayed hyperbaric
oxygen treatment on ischemic brain injury of rabbit
Zhaopan Lai,
Zhi Liu,
Gaoyu Cui,
Jiangkai Lin,
Xianrong Wang, Hua Feng
Department of Neurosurgery, Southwest Hospital, Third
Military Medical University, Chengdu, China
Objectives: To investigate the effect of early and delayed
hyperbaric oxygen (HBO) treatment on local ischemic
brain injury in rabbit. Method: 32 rabbits were randomly
divided into 4 groups: control group(sham), middle
cerebral artery obstruction (MCAO)group, MCAO+HBO
group, and MACO+DHBO group(delayed HBO started at
7 days after MCAO). 2 atmosphere pressure HBO was
applied for 1 h per day, and neurological score, TTC
dyeing and pathomorphological observation were
performed to evaluate the effect of early and delayed
HBO treatment. Result: Neuroethological abnormalities
could be observed in all experimental groups except
control group. 30 d after obstruction, ethological score
showed rabbits in MCAO+HBO group was better than
MCAO+delayed HBO (P<0.05); TTC dyeing showed the
infarct size of MCAO, MCAO+HBO and MCAO+delayed
HBO were 378±35.6 mm3, 59±17.2 mm3 and 139±45.8
mm3, respectively, with a significant difference in
MCAO+HBO group compared with other two groups
(p<0.01). Neuron edema, degeneration and decrease in
cell number were observed with HE staining, which was
more server in MCAO group and indistinct in MCAO+HBO
group. Conclusion: Early HBO treatment has an
advantage on the local ischemic brain injury of rabbit
compared with delayed HBO.
K35
The effect of HBO on survival of the arterialized
deep-venous flaps in a rat model
Ying LONG,
Junjun HE,
Jiewen TAN,
Xiaojun LI,
Yulin NIE, Zhi YI
1. The Shenzhen Peoples Hospital, Shenzhen, China
2. The Second Affiliated Hospital of Sun Yat-sen
University, Guangzhou, China
3. The Buji Peoples Hospital, Shenzhen, China
Objectives: the effect of hyperbaric oxygen therapy (HBO)
on survival of the arterialized deep-venous flaps and
blood supply was studied in a rat model. Methods: In this
study, the arterialized deep-venous flaps with pedicle
were established and anastomosed by proximal end of
external iliac artery with external iliac vein of flaps on the
abdomen of rats. An experimental model was created in
retrograde blood supplied to flaps from external iliac vein
inferior and epigastric vein. The 45 of SD rats were
randomly divided into three groups. The blood supply of
flaps in Group I (Control, n=15) was anastomosed by
end-to-end with arteries and venous respectively. 30 of 45
rats were anastomosed in arterialized deep-venous flaps
of rat model(ADVF). 15 rats with ADVF (GroupII, HBO)
were received by HBO treatment at 0.2MPa twice daily for
3 consecutive days after operation. Another 15 of
ADVF(Group III , non-HBO) were received by
conventional treatment after operation. Clinical survival
assessments of flaps included perfusion monitored by
observation of mass species with naked eye. The survival
rate of flaps was calculated after operation. Result: 1.The
edema of flaps in ADVF was reduced by HBO . The color
of flaps after HBO was red in the similar to Group I. The
Flaps in Group III were going to be black red from 2. day
after operation and dying on 3.day after operation. 2.The
survival of the flaps were respectively 60%, 66.7%, 0.
Conclusion: This study indicates that it iseffectiveness of
blood circulation in this arterialized deep-venous flaps can
be enhanced by retrograde arteriali- zation in this animal
model. HBO could improve blood supply of the flaps with
ADVP and reduce edema after operation and increase
flap survival with ADVP.
K36
Cancelling the requirement of ventilation for
hyperbaric chamber pressurized with oxygen
Mao Fangguan
Chinese Society of Hyperbaric Facility, Shanghai, China
Objective: When the volume fraction of oxygen of gas in
a chamber is reduced the potentiality of fire will decrease
and the operational safety will increase for the hyperbaric
chamber pressurized with oxygen. Methods: By means of
analyses and calculations the maxima of oxygen patial
pressure (pO2) and carbon dioxide partial pressure (pCO2)
in the chamber can be determined under conditions of
nonventilation. Results: In this situation the pO2 may be
raised to 0.22 MPa, it can meet the demands of HBO
conventional therapy and the maximum of pCO2 less than
3 kPa is safe on the human body. Conclusion: It is
favorable and reasonable to cancel the requirement of
ventilation for a hyperbaric chamber pressurized with
oxygen.
K37
Reports of 48 cases about hyperbaric oxygen
approach in treating thromboangiitis obliterans
Jinjun Pang, Fengjuan Long, Guihua Pan, Yafan Li
First Affiliated Hospital, Guangxi Medical University,
China
Objective: To explore the effects of hyperbaric oxygen on
thromboangiitis obliterans (TAO). Method: There are 48
cases in our study.8 cases in stageⅠ,18 cases in stage Ⅱ
and 22 cases in stage Ⅲ .All cases were treated by
hyperbaric oxygen. Medicine was applied to severe cases
We compare the effects in different stage and course of
treatment. Results: 37 cases were cured,10 cases were
effective, The total effective rate was 97.9%.26 cases in
stageⅠ or stage Ⅱ were completely cured, the cure rate
was 100%.The cases the cure rate was only 50% in stage
Ⅲ .36 cases accept 4 courses hyperbaric oxygen
treatment, 33 cases were cured, the cure rate was
91.7%.12 cases were treated less than 4 courses; the
cure rate was 25%. Conclusions: Hyperbaric oxygen has
a notable curative effect on TAO. We can receive high
cure rate if we adopt complete courses hyperbaric oxygen
treatment in stageⅠ and stage Ⅱ.
K38
Hyperbaric Oxygenation on Limb Dyskinesia after
Intervention of intracranial Aneurysm and Its Effects
on ADL among 32 Cases
Yongjun Mai, Xuedong Li, Meiyin Lei, Hua Huang
HOB center, the Fourth Affiliated Hospital, GuangXi
Medical University, Liuzhou, China
Objective: To determine the effects of early hyperbaric
oxygenation (HBO) on the prognosis of motor function
after intervention of intracranial aneurysm. Methods: 62
patients with dyskinesia after intervention of intracranial
aneurysm were randomized into HBO treatment group
and control group. Both group received the same routine
th
th
medicine. In addition, HBO group started HBO at 7 –10
day after intervention. Defects of nerve function and ADL
were evaluated with Fugl-Meyer Scale and Barthel Scale
respectively. Results: Neurofunction defects and ADL
were significantly improved 30 days after treatment in
both groups. There was statistic significance compared
with the conditions after interventions (P<0.01). The
results of HBO group were much better than those of
control group. There was statistic significance between
the two groups (P<0.01). Conclusion: Early HBO can
improve patients’ ADL and defects of nerve functions after
intracranial aneurysm intervention, which enhances
patients’ life quality.
K39
The EEG analysis before and after of hyperbaric
oxygen therapy in patients with cerebral infarctinon
Ma YuJie
No.1 Hospital, Qiqihaer, China
Objective: Analysis and Comparison of patients with
cerebral infarction in HBO treatment before and after the
EEG changes, study the prognosis of the disease. HBO
treatment can reduce the incidence of meaningful
consequences. Methods: HBO group in the conventional
treatment at the same time increase the HBO therapy.
HBO use large cabin wearing oxygen masks in 30″×2, the
middle cabin air suction, once a day, 10times for a course
of treatment, 2-3 courses of treatment. Conventional
treatment of the control group, using low-molecular-weight
dextran 500ml and dan senate 14 ml; 5%glucose injection
250 ml and CDP- choline 0.5g intravenous drip, once a
day, seven days for treatment, treatment 2-3 Courses.
Results: HBO group EEG abnormalities before treatment
to 79 percent, 51 percent after treatment, down
28%,compared before and after treatment were
significantly different (P<0.05)； The control group EEG
abnormalities before and after the treatment rate of 80
percent, reduced to 66 percent, down 14 percent; no
significant difference (P>0.05). Conclusions: HBO can
improve the prognosis of cerebra infarction patients,
whether from the clinical efficacy or EEG of recovery, the
effect is very obvious that the lower incidence of sequelae
and provide a reliable basis for the treatment. So routine
treatment of patients with cerebral Infarction at the same
time as soon as possible with HBO treatment.
K40
Influence of hyperbaric oxygen to the immune
function of cells in the patients with chronic
obstructive pulmonary disease
1
2
1
1
Bing Meng , Yongxin Ma , Guohua Li , Guiping Wang ,
1
Xiaorong Liang
1.Weifang asthma hospital, Shandong, China
2.Weifang people’s hospital, Shandong, China
Objective: To Observe the level of immune cells in the
patients with chronic obstructive pulmonary disease and
the influence of hyperbaric oxygen therapy to the immune
function of cells in the stability of patients with chronic
obstructive pulmonary disease. Methods: Randomly
selected patients with stable COPD 167 cases, of which
86 cases for the treatment group, 81 cases for the control
group, Tiotropium inhalation aerosol solution, selected 33
cases of normal healthy people in the control group.
Adoption of improved ABC method in the Observer Group
targets immune cells, blood testing, pulmonary function,
after a course of HBO treatment (12 times), to detect the
above targets again, and then to give a comparative study
analysis. Results: HBO inhalation drug therapy group
and the control group compared with the normal group
targets immune cells have different levels of reduced (P
<0.001), the HBO treatment group after treatment,
cell-mediated immunity in patients with various indicators
were significantly increased (P <0.01) , and corrected the
CO2 retention and hypoxia (P <0.01), improved lung
function. Conclusion: the level of immune cells in the
stability of patients with COPD is degraded in varying
degrees, the reduction of HBO treatment can significantly
improve the stability of the cell-mediated immunity in
patients with COPD function, at the same time to correct
hypoxia and CO2 retention and improve lung function in
patients with COPD (P <0.01).
K41
Effect of hyperbaric oxygen to inflammatory cytokine
in patients with obstructive sleep apnea-hypopnea
syndrome
1
2
2
Yongxin Ma , Nong Yu , Bing Meng
1.Weifang people’s hospital, Shandong, China
2.Weifang Asthma hospital, Shandong, China
Objective: To observe the effect of hyperbaric
oxygen(HBO) to inflammatory cytokine in patients with
obstructive
sleep
apnea-hypopnea
syndrome(OSAHS).Methods: Thirty two patients with
OSAHS were randomly divided into 2 groups: HBO
therapy group and control group. The T lymphocyte
+
+
+
+
subset,CD4 ,CD8 ,CD4 /CD8
and
inflammatory
cytokine,IL-6,IL-8as well as TNF-α,were assayed by flow
cytometry after HBO exposure. Result: The level of CD4+
and CD4+/CD8+ ratio tended to increase after HBO
exposure 7 times, while significantly decreased (P <0.001)
after HBO exposure 14 times and 21times. The level of
inflammatory cytokine,IL-6,IL-8 and TNF-αtended to
decreased (P <0.005) after HBO exposure 7 times, and
significantly decreased too (P <0.001) after HBO
exposure 14 times and 21times. Conclusion: HBO
therapy can not only adjust the balance of immune,
+
decreased the cytotoxic action of CD8 lymphocyte, but
also significantly decrease the level of inflammatory
cytokine in patients with OSAHS.
K42
Effect of hyperbaric oxygen to inflammatory cytokine
and pulmonary function in patients with Asthma
1
2
2
Yongxin Ma , Zhaoqiu Hu , nong Yu
1. Weifang Peoples Hospital, Shandong, China
2. Weifang Asthma Hospital, Shandong, China
Objective: To observe the effect of hyperbaric
oxygen(HBO) to inflammatory cytokineand pulmonary
function in patients with asthma. Methods: ninety six
patients with asthma were randomly divided into 2 groups:
HBO therapy group and control group, and another thirty
health cases as heath control group, the control group
only accept regular therapy: To inhale Budesonide 200µg
2 times daily, and HBO group accept HBO therapy and
regular therapy too. The inflammatory cytokine IL-5, IL-12,
IL-13 as well as IL-18 were assayed by ELISA after HBO
exposure. Result: The symptom and FEV1 were
significantly improved after HBO exposure(P <0.005) ,the
level of IL-5, IL-13 ,IL-18 were significantly decreased ,
the level of IL-12 was significantly increased. Conclusion:
HBO therapy can ease the bronchial inflammation by
adjust the inflammatory cytokine excretion, can
significantly improve the patient’s pulmonary function.
K43
The effect of HBO treatment on plateletm embrane
glycoprotein
Ma Linlin
Beijing Chaoyang Hospital, Beijing, China
Objective: Study the changes of HBO therapy on
plateletm embrane glycoprotein, to further reveal the
mechanism of HBO therapy and provide a theoretical
basis with use anti-platelet aggregation in clinical.
Methods: Through animal tests and clinical studies on the
HBO treatment of common indications (Acute carbon
monoxide poisoning and delayed encephalopathy, acute
oxygen poisoning, decompression sickness, ischemic
stroke, ischemia-reperfusion injury, SARS after avascular
necrosis), the positive percentage of CD31, CD61, CD62p,
CD63 and PAC-1 were measured by the flow cytometry
(FCM). Results: HBO on platelet activation remains
differences. Most studies show that HBO will reduce
platelet glycoprotein expression, while a small number of
studies have appeared that HBO can increase the
expression of the plateletm embrane glycoprotein to
The dual nature of HBO
normal. Conclusions:
treatment pends further study. HBO treatment with the
pressure of time, intermittent, and the treatment of the
disease itself? With further study of HBO on platelet
glycoprotein, the exact mechanism will become more
deeply clarified.
K44
The influence of HBO on plasma vWF, serum IL-23
and IL-27 in cerebral ischemic-reperfusion rats
Jiangjiang Lu, Chunjin Gao, Yajuan Li, Baosen Pang, Xiu
xia Huang
Beijing Chao Yang Hospital, Beijing, China
Objective: By observing the influence of hyperbaric
oxygen treatment on plasma vWF, serum IL-23 and IL-27
in cerebral ischemic-reperfusion rats, to explore possible
mechanism of hyperbaric oxygen treatment on relieving
ischemic-reperfusion flammatory reaction and to provide
further experimental evidence for clinical HBO therapeutic
method. Materials and Methods: 64 SD rats were
randomly divided into 8 different groups: normal group (N),
sham-o group, cerebral ischemic-reperfusion (IR) IR 24
hour’s group, IR 48 hour’s group, IR 72 hour’s group, HBO
24 hour’s group, HBO 48 hour’s group, HBO 72 hour’s
group. Each group contained 8 rats. The cerebral
ischemic-reperfusion models were established according
to Pulsinelli’s methods of blocking four arteries. The
normal
group,
sham-o
group,
cerebral
ischemic-reperfusion (IR) group was placed in normal
pressure atmosphere without hyperbaric oxygen
treatment. The hyperbaric oxygen group started to accept
hyperbaric oxygen treatment after surgery immediately,
and once a day at the same time. HBO 24 hours group got
once HBO treatment. HBO 48 hours group got twice HBO
treatment. HBO 48 hours group got three times HBO
treatment. Draw blood from the aorta abdominalis after
operation at 24h, 48h, 72h respectively, then measure the
content of vWF, IL-23 and IL-27 by using enzyme-linked
immunosorbent assay (ELISA). Results: (1) The content
of vWF in IR 24 hours group increases than that in
sham-o group, the difference is significant, P in 24 hours
group of IR increases than that in sham-o group, the
difference is significant, P in 24 hours group, 72 hours
group of HBO group decrease than those in IR groups at
the same time group, the difference is significant, P in 24
hours group, 48 hours group, 72 hours group of HBO
group decrease than those in IR groups at the same time
group, but the difference is not significant, P>0.05.
Conclusions: (1) By using hyperbaric oxygen, the
content of vWF decreases at 24h, 48h and 72h, it shows
that the hyperbaric oxygen has positive effect on relieving
ischemic-reperfusion injury. The mechanism is to
decrease the content of vWF. (2) By using hyperbaric
oxygen, the content of IL-23 decreases at 24h and 72h, it
shows that hyperbaric oxygen has positive effect on
inhibiting inflammatory reaction and the mechanism of
hyperbaric oxygen is to alleviate the IL-23 release.(3) In
this experiment, by using hyperbaric oxygen, the content
of IL-27 has the degressive trend, but has no significant
meaning. It needs further study.
K45
Effects of preconditioning with hyperbaric oxygen on
neural cell apoptosis after spinal cord injury in rats
Peigang Lu, Hua Feng, Yongzhi Xia, Guoqi Gong, Mei Li
Department of Neurosurgery, Southwest Hospital, Third
Military Medical University, Chengdu, China
Objective: To study the effects of preconditioning with
hyperbaric oxygen on neural cell apoptosis and
expression of Caspase-3 after spinal cord injury in rats at
different time. Methods: fifty-five adult Sprague-Dawley
adult rats, weighing from 250 to 300g, were randomly
divided into 3 groups, hyperbaric oxygen preconditional
group(25,n=5) ,normal injury group(25,n=5)and control
group(5,n=5). In the experimental groups, the rat spinal
cord injury models were established by the way of
,
Allen s method , and morphological studied by
hematoxylin and eosin staining, Cell apoptosis was
detected by TUNEL method and the expression of
Caspase-3 by immunohistochemistry at different time(1
day, 5 day, 7 day, 10day, 14day) were observed. Results:
The apoptostic cells positive for TUNEL and Caspase-3
expression were detectable in both two experimental
groups. In the preconditional group, the number of
apoptotic cells and the expression of Caspase-3 were
decreased and the neurofunction of the spinal cord
improved as compared with those in the control group.
There were statistically significant differences between
the preconditional group and normal injury group
especially the control group. Conclusion: Apoptosis of
neural cells is an important morphological change in the
secondary lesion period after spinal cord injury. HBO
preconditioning can reduce the numbers of apoptotic cells
and promote the nerve function recovery in rats after
spinal cord injury.
1. Department of Pediatrics, the 3rd Xiangya Hospital of
Central South University, Hunan, Changsha, China
2. Navy General Hospital, Beijing, China
K46
Automatic and non-resistance and non-noise
oxygen-supply-and-vent device
Shutian Duan, Xianhua Zhang, Binsheng Ren
People’s Hospital, Pingyao County, Shanxi, China
Objective: To reduce resistance and noise and avoid
cross infection in hyperbaric oxygen therapy. Method:
Place the detector outside the cabin .Place the air sac and
silencer in the cabin. Use the detector to detect whether
the air sac is full or not in order to follow the human body
breath, and supply or vent oxygen .Result: The
resistance of breathing is largely reduced. The resistance
of calm breathing can almost become zero. The noise is
greatly reduced. This can avoid cross-infection.
Conclusion: This kind of oxygen-supply-and-vent device
can be used in hyperbaric oxygen therapy.
K47
Determination of platelet membrane glycoprotein
PAC-I and CD63 in patients with delayed
ecefhalopathy after acute carbon monoxide poisoning
Xiaomin Hou, Chunjin Gao, Huan Ge, Liming Zhao, Lianh
ua Wu, Yi Zhang, Lin Yang, Zhuo Li, Yu Gao, Man Qi, Ch
engqing Xia
Beijing Chaoyang Hospital, Beijing, China
Objective: To study platelet membrane glycoprotein
PAC-I and CD63 in patients with delayed ecefhalopathy
after acute carbon monoxidepoisoning in order to instruct
the anti-platelet therapy of DE ACMP. Methods: There
are DEACMP group and normal control group. The
DEACMP group were draw blood in 7 days after they
arrived at hospital. The blood of normal control
group were drawn in the 8AM after an overnight fast. The
positive percentage of PAC- I and CD63 were measured
by the flow cytometry (FCM). Analysis of t-test was
applied to analyse the expressions of platelet membrane
glycoprotein PAC-I and CD63. Results: The expressions
of platelet membrane glycoprotein PAC- I of the
DEACMP group was higher than which in the normal
control group (P<0.05). The expressions of platelet
membrane glycoprotein CD63 of the DEACMP group was
much higher than which in the normal control group
(P<0.01). Conclusions: According to this study, the
expression of PAC-I and CD63 is in a high level with
delayed
ecefhalopathy
after
acute
carbon
monoxidepoisoning. It indicates that the inhibition of
platelet activation is needed with delayed ecefhalopathy
after acute carbon monoxidepoisoning.
K48
Effect of hyperbaric oxygenation to human nerve
stem cell transplantation on hypoxic ischemic brain
damage in vivo
1
1
2
Mingyan Hei , Mili Xiao , Zuo Luan
Objectives: To observe the effect of Hyperbaric Oxygen
(HBO) to Human Nerve Stem Cell (hNSCs)
transplantation on hypoxic Ischemic Brain Damage (HIBD)
on neonatal rats. Methods: Neonatal HIBD rat model was
produced according to Rice method. Experimental rat
pups
were
randomly
divided
into
HIBD,
HIBD+transplantation
group,
HIBD+HBO
and
HIBD+transplantation+HBO groups (n=10 in each group
at each time point). On day 3 (d3) after HIBD, hNSCs
were transplanted into the left ventricles by microinjection
method. The first HBO treatment was administered within
15min after hNSCs transplantation and once daily for
consecutively ten days. TUNEL staining was used for
apoptotic study at d4, d5, d6, d13 after HIBD. Fluorescent
immunohistochemical (FIHC) staining and confocal
microscopy were used to observe the survival, migration,
and differentiation of the implanted cells. Results: (1) On
d4, d5, d6 and d13, TUNEL-positive cells could be
observed in the injured cortex and hippocampus in all
groups. The number of TUNEL-positive cells of
HIBD+transplantation and HIBD+HBO groups were
significantly less than that of HIBD group (P<0.05). While
the
number
of
TUNEL-positive
cells
of
HIBD+transplantation+HBO group was very significantly
less than that of HIBD group (P<0.01). (2) FIHC staining
showed that on d13, the implanted hNSCs survived and
extensively migrated from ventricles to periventricular
brain parenchyma, and meanwhile differentiated to
neurons and astrocytes. There was no significant
difference about the cell migration style and distribution
among
HIBD+transplantation,
HIBD+HBO
and
HIBD+transplantation+HBO groups. The number of total
survived cells of HIBD+transplantation+HBO was
significantly
(P<0.01)
more
than
that
of
HIBD+transplantation group or HIBD+HBO group.
Conclusions: (1) After transplantation, hNSCs can
survive, migrate and differentiate into neurons and
astrocytes. (2) To some extent, hNSCs transplantation
has neuroprotection to HIBD in neonatal rats. (3) HBO
therapy may enhance the above neuroprotective effect via
promoting graft cell survival rate, migration and
differentiation.
K49
Hyperbaric oxygen therapy for a case of gas
gangrene in both feet
He Xia
Hyperbaric oxygen department of Chongqing Emergency
Medical Center, Chongqing, China
Objective: Through HBO therapy the gas gangrene.
Methods: Air hyperbaric chamber, pressure: 2.5ATA
compression time for 40minutes, inhal oxygen for 30
minutes, then 10 minutes’ break. After that, another 30
minutes’ oxygen inhalation. Further, 50 minutes for
decompression with outing of chamber. During the above
procedure, no uncomfortable sense wascomplained.
Results:
After3hours:T:39C,HR:140bpm,R:30/min,BP:128/58mmH
g.After 12 hours, the patient happened tachypnea. T: 39.2
℃HR: 157bpm, R: 35/min, BP:112/98 mmHg. The patient
cough pink frothy sputum and the both base of lung had
moist rale. Thoracic CT: ⑴ pneumocontusion and
pneumonedema ⑵pulmonary infection⑶pleural effusion.
Conclusion: HBO has therapeutic effect on gas
gangrene pneumonedema. The condition of patient
aggravate suddenly may have relation to these factors:
⑴The patient have no x –ray from admission-time to
going to compartment. It unknown whether decompress
conditional
will
induce
reversion
under
the
pneumonedema. ⑵The systemic inflammatory response
x-ray
of
syndrome
was
obvious. ⑶ Chest
pneumocontusion without clinical syndrome was regarded
as contrary contraindecation abroad. The patient
sustained trauma and change of lung x –ray. It can not
exclude out pneumocontusion.
K50
Effects of hyperbaric oxygen on expression of brain
derived neurotrophic factor mRNA in hippocampus of
mice after cerebral ischemia reperfusion
Na Guo, Chunjin Gao, Guozhong Wang, Fujia Liu, Yuepi
ng Lu
Chaoyang Hospital, Beijing, China
Objective: To investigate the effects of hyperbaric
oxygen (HBO) on expression of brain derived
neurotrophic factor (BDNF) mRNA in hippocampus of
mice after cerebral ischemia reperfusion and explore the
therapeutic mechanism of HBO from molecular level.
Methods: The mice were randomly divided into a cerebral
ischemia reperfusion group (IR group), a hyperbaric
oxygen group (HBO group) and a sham–operation group
(Sham group). The cerebral ischemia reperfusion models
were established. The expression of BDNF mRNA in the
hippocampus of mice was measured at 4 h, 12 h, 24 h, 48
h and 72 h respectively, after reperfusion by reverse
transcription- polymerase chain reaction (RT–PCR).
Results: 1) The expression of BDNF mRNA in
hippocampus in HBO group was higher than those in the
Sham group at 4 h, 12 h, 24 h, 48 h and 72 h (P0.05).3)
The expression of BDNF mRNA in HBO group was lower
than that in IR group at 4 h (P0.05). Conclusion: The
expression of BDNF mRNA increased in hippocampus of
mice after cerebral ischemia reperfusion. HBO treatment
may effectively relieve the cerebral ischemia reperfusion
injury through improving the expression of BDNF mRNA
in hippocampus of mice after cerebral ischemia
reperfusion.
K51
The efficacy and nursing research of
dysarthria’ therapy by hyperbaric oxygen
Guo Junmei
Second Hospital, Shenzhen, China
stroke
Objective: The efficacy of strocke dysarthria’ therapy by
hyperbaric oxygen. Methods: from 2005 to 2006, 60
patients have divided into two groups.30 patients with the
hyperbaric oxygen therapy is the observation group, the
other is the control group. 60 patients with the
implementation of conventional therapy, the Observation
group have the hyperbaric oxygen therapy at the basic
therapy. Results: The effective rate of observer Group is
90%, 53.33% control group. The therapeutic effect of the
two groups was significant (P<0.05). Conclusion:
hyperbaric oxygen therapy can improve stroke dysarthria
efficient.
K52
Hyperbaric oxygen treatment of persistent vegetative
state 28 cases Experience
Guo Guanghan
Suqian City of Jiangsu Province Peoples Hospital, Suqian,
China
Objective: Study the effects of hyperbaric oxygen on the
persistent vegetative state patient rehabilitation treatment.
Methods: 2001-1/2007-12 Suqian City People's Hospital
of hyperbaric oxygen treatment centre-patient treatment
of 28 cases of pvs patients receiving hyperbaric oxygen
rehabilitation treatment, using high-pressure air cabin, 0.2
mpa pressure, oxygen for 60 min, 10 min middle rest.
Results: The rehabilitation of hyperbaric oxygen therapy,
basic rehabilitation eight cases, for more then 80 times in
6 cases, treatment within 60 to two cases, in the course
within 30d by hyperbaric oxygen therapy in 12 cases, the
basic rehabilitation of six cases in the 60d via the high
oxygen therapy the basic rehabilitation of two cases. 28
cases of cases of patients 12-40 years of age, the basic
rehabilitation of six cases ,41-61-year-old with 10 cases,
the basic rehabilitation of two cases, patients over of 61
years of age without basic rehabilitation. Conclusion:
Hyperbaric oxygen therapy can be recovered in blood
pressure, improve microcirculation, help improve
persistent vegetative state patients awake and function to
maintain life.
K53
Using Hyperbaric Oxygen as an Adjuvant Method in
Hypersensivisity Vasculitis
Huan Ge, Chunjin Gao
Department of Hyperbaric Oxygen, Beijing Chaoyang
Hospital, Capital University of Medical Sciences, Beijing,
China
Aim: We report a case who was suffered from
hypersensivisity vasculitis. Using Hyperbaric Oxygen and
corticosteroid to treat hypersensivisity vaasculitis. To the
best of our knowledge, this is the first report that
hyperbaric oxygen (HBO) served as an adjunctive therapy
for hypersensitivity vasculitis. Method: A 58-year-old
male patient was injected into his thumb of right hand
because of tenosynovitis in local hospital. Half an hour
later, he started to have pain and swelling in his distal
upper extremity of right side. Some medicine were
prescribed and for 4 hours without benefit.New lesions
developed on the elbow, as well as on the hand. Palpable
Purpura was involving anterior aspect of the left upper
limb through to elbow. Darkening of the skin also were
noted. That night patient was transferred to Beijing
Chaoyang Hospital affiliated Capital University of Medical
Science.Tolazoline 20mg im and Dextran 40 500ml iv
were used to treat spasms of peripheral blood vessels
and improve microcirculation by decreasing blood
viscosity and impeding erythrocyte aggregation.
Dexamethasone meanwhile intravenously 20mg was
given. Two hours later the patient’s condition deteriorated
and his lesions developed. HBO therapy (0.2Mpa) was
used to alter condition of local hypoxia. After HBO therapy,
the patient’s situation was improved obviously. His
palpable purpura did not reappeared until 3 hour later. At
the first 24 hours after admission, the patient was treated
with HBO therapy twice. Subsequently, he received HBO
therapy 12 sections (once a day). From the second day,
he was orally given 40mg of prednisolone and 200 mg of
Aspirin per day for 10 days. Result: The patient’s
condition was getting better, and palpable purpura and
darkening of the skin were also reduced gradually. Fifteen
days after treatment with HBO and cortin, the patient’s
symptoms and ultrasound finding were normalized. He
have a decrease in the dose of prednisolone 30mg/d for
10 days, then 20mg/d until discharge. Conclusion: HBO
therapy combine with small doses of corticosteroid is an
effective and safe therapy for this patient, further studies
are needed to evaluate whether HBO can be used as
primary therapy for a group of patients suffering from
hypersensitivity vasculitis.
K54
Clinical effects of HBO treatment in Patients with
Mental Disorder Caused by TBI using of BPRS and
HDS and ADL
1
1
1
2
Zhaoming Ge , Ling Li , Hongbin Cai , Xudong Zhang ,
1
Haijun Ren
1. Second Hospital of Lanzhou University, Lanzhou,
China
2. Gansu Provincial Peoples Hospital, Lanzhou, China
Background: To evaluate clinical effects of hyperbaric
oxygen (HBO) treatment in patients with Mental Disorder
arising from traumatic brain injury. Methods: One
hundred and five patients with Mental Disorder following
Traumatic Brain lnjury (TBI) were randomly divided into
therapy group(55 cases), which was treated by hyperbaric
oxygenation and medications ,and control group (50
cases) which was treated by medications. The scales
included the Brief psychiatric rating scale (BPRS) and
Hasegawa Dementia Scale (HDS) and Activity of Daily
Living Scale (ADL).All patients were evaluated with BPRS
and HDS and ADL before and after treatment. Results:
Obvious significance in BPRS and HDS and ADL scores
existed between two groups (after two course of treatment
P <0.05, after the four course and three month P <0.01).
Conclusion: The combination of HBO shows good
therapeutic efficacy to patients with Mental Disorder
caused by traumatic brain injury.
K55
Effect of hyperbaric oxygen combined therapy on
persistent vegetative state in 30 patients.
Gao Yu, Gao Chunjin, Ge Huan
Beijing Chaoyang Hospital, Beijing, China
Objective: To evaluate effectiveness of hyperbaric
oxygen combined therapy on persistent vegetative state
and explore the best therapeutic regimen for it. Method:
Thirty cases of persistent vegetative state treated with
hyperbaric oxygen combined therapy from 1999 to 2005
were analyzed retrospectively. Including the effects of
etiological factors and treatment times and treatment
juncture on therapy. Result: The excellence rate and
improvement rate were significantly higher in cerebral
trauma group than those in no cerebral trauma group. And
two groups in which the patients began to receive
treatment when became persistent vegetative state for
less 61 days recovered better than the other one in which
the patients began to receive treatment when became
persistent vegetative state for more 60 days.
Conclusions: The effectiveness of hyperbaric oxygen on
the patients with persistent vegetative state caused by
cerebral trauma and the patients who just begin to receive
hyperbaric oxygen treatment when having been ill for less
61 days better.
K56
Efficacy of hyperbaric oxygenation therapy for
diabetic foot
Bi Zhumei
Shandong Provincial Hospital, Jinan, China
Objective: To study the efficacy of hyperbaric
oxygenation therapy(HBO) on patients with diabetic foot.
Methods: 42 patients were randomly divided into
conventional therapy group and HBO therapy combined
group, efficacy differences between the two groups was
compared. Results: Cure rate and total effective rate was
68.2% and 86.4% in HBO combined group while it was
20.0% and 60.0% in the control group , differences were
seen between the 2 groups obviously (P<0.05).
Conclusion: HBO therapy augments the efficacy of
conventional therapy.
K57
Effects of comprehensive hyperbaric oxygen
rehabilitation
on
function
recovery
for
cerebralapoplexy patient
NingQin Gao, XiaoPing Chen, Jun Shen, Ying Zhang, Ta
oLi Wang, LiHua Huang, HaiPing Ge
Xuhui Central Hospital, Shanghai, China
Purpose: Discuss the effects of comprehensive
hyperbaric oxygen rehabilitation on function recovery for
cerebralapoplexy patient. Method: 50 cerebralapoplexy
patients are divided into two groups, namely, hyperbaric
oxygen and non-hyperbaric oxygen group. The routine
treatment and rehabilitation is taken for both two groups
and the additional hyperbaric oxygen treatment is
provided for hyperbaric oxygen group. The functional
independence measurer (FIM) of patient and simplified
Fugl-Meyer motion function evaluation method can be
used for evaluation. Results: The conclusion shows that
the FIM and simplified Fugl-Meyer scoring difference
between both two groups have the statistics significance
after treatment and the hyperbaric oxygen group is
superior to non-hyperbaric oxygen group. FIM is
100.92±15.76 after the patient in hyperbaric oxygen group
is treated and FIM is 83.60±28.20 after the patient in
non-hyperbaric oxygen group is treated. The comparison
between two groups P±22.61 after the patient in
hyperbaric oxygen group is treated and the simplified
Fugl-Meyer is 36.64±24.26 after the patient in
non-hyperbaric oxygen group is treated. The comparison
between two groups P<0.01. The simplified Fugl-Meyer is
54.48±22.61 after the patient in hyperbaric oxygen group
is treated and the simplified Fugl-Meyer is 36.64±24.26
after the patient in non-hyperbaric oxygen group is treated.
The comparison between two groups P<0.01
Conclusions: The conclusion is that the hyperbaric
oxygen treatment can improve the activities of daily living
and motion function of cerebralapoplexy patients.
K58
Systematic review of the effects of hyperbaric oxygen
adjunctive therapy on brain injury
Li Chu, Shilian Hu, Oyang Aiqun, Yaowu Ge, Wang Qiang
The Second Affiliated Hospital of Anhui Medical University,
Hefei, China
Objective: To evaluate the effects of hyperbaric oxygen
（HBO）combined therapy on the patients with brain injury.
Methods: HBO, as a commonly-used adjuvant
therapeutic method to treat the brain injury, is not
unanimously accepted by the doctors yet. In this paper,
we searched for the domestically published studies on the
HBO combined therapy on brain injury and only those
studies in accordance with the following inclusion criteria
were reviewed systemically. ⑴ Randomized controlled
trials (RCTs) and clinical controlled trials (CCTs) about the
HBO combined therapy on brain injury without
confounding factors. ⑵ Trials should contain control
groups (medicine/operation combined with HBO) and
treatment groups (treated with medicine/operation only).
No less than 100 cases were needed in each treatment
group and the case number in control group must meet
the statistical requirements. ⑶ The age of each case
should be between 9 to 75 years old. Statistical Treatment:
The statistics software RevMan4.2 provided by Central
Cochrane was applied to systematically review the effects
of HBO combined therapy on brain injury. Odds Ratio (OR)
was applied and 95% Confidence Intervals (95%CI) were
calculated. Chi square test was also carried out to assess
the heterogeneity among groups. Results: Up to March
2008, 623 papers about the effects of HBO combined
therapy on brain injury were browsed and only 9 trials
(involving 3916 cases) in accordance with the inclusion
criteria were reviewed systemically. Among these 9 trials,
total effective rates were compared in 8 trials, the scores
of Glasgow Coma Scale (GCS) before treatment and one
month after treatment were compared in 2 trials, and the
mortality rates of the cases within 6 months’ follow-up
study after treatment were compared in 2 trials. After the
statistical treatment with the statistics software
RevMan4.2, the following statistical outcome was
obtained. ⑴ The total effective rate: Clinical effects were
reported in 8 trials (involving 3688 cases), and the total
effective rate of HBO combined treatment groups was
markedly improved with statistical significance as
compared with that of control groups (95.12% vs. 74.85%;
OR 6.37; 95%CI 5.04,8.06; P<0.01). ⑵ The comparison
of the GCS scores: The GCS scores of cases had no
statistical significance among groups in 2 trials (involving
430 cases) before treatment and there was no
heterogeneity among groups after Chi square test
(P=0.22), but the combining GCS score of cases one
month after treatment in treatment groups was 1.67 more
than that in control groups one month after treatment and
the difference had statistical significance (95%CI 1.2,2.14;
P<0.01),. ⑶ The mortality rates of the cases 6 months
after treatment: The mortality rates of cases within six
months’ follow-up study after treatment were carried out in
2 trials with 420 cases involved and there was no
heterogeneity among groups after Chi square test
(P=0.13). It was found that the total mortality rate of cases
in 6 months after treatment was 10.76%(24/223) in
treatment groups, which was less than the total mortality
rate (18.78%; 37/197) of cases in control groups with
statistical significance (10.76% vs. 18.78%; OR 0.51;
95%CI 0.29,0.90; P＜0.05),. And NNT(Number Needed to
Treat) was 12.5，which suggests that one patient with
brain injury could be avoided from death in 6 months
among every 12.5 patients if adjunctively treated with
HBO. Conclusion: After the systemic review of the
studies of HBO combined therapy on brain injury, it could
be found that the total effective rate significantly improved
and the mortality rate obviously decreased after patients
with brain injury had been adjunctively treated with HBO.
So, HBO therapy is a desirable treatment measure for the
patients with brain injury, for it proved to be of definite
therapeutic effects and clinical significance.
K59
Clinical study of hyperbaric oxygen treating in
patients with developing cerebral infarction
Du Yuqin
Shantou University Medical College First Subsidiary
Hospital, Shantou, China
Objective: To explore the therapeutic effects and safety
of Hyperbaric Oxygen treating in patients with developing
cerebral infarction. Methods: 63 patients were randomly
divided into two groups. The control group (n=30) was
treated with Xue Shuan Tong and conventional therapy
for ACI , and the treatment group (n=33) was treated with
Hyperbaric Oxygen treatment combined with conventional
therapy for ACI , general for two weeks. Results: After the
above-mentioned therapy , the scores of Nervous
Functional Deficiency were lower than before therapy and
the parameters of blood viscosity were decreased in two
groups , but were significantly progress and decreased in
the treatment group compared with the control
group (P<0.01or
P<0.05).
Conclusion: Hyperbaric
Oxygen treatment is a safe and effective method in
patients with developing cerebral infarction.
K60
Nursing experience in treating h2s poisoning with
high pressure oxygen
Chen Sailian
Department of HBO, the People’s Hospital of Tongling city,
Anhui, China
To treat H2S poisoning, high pressure oxygen therapy
should be used as early as possible. High concentration
oxygen inhalation; continuous positive pressure breathing,
artificial sputum, avoiding respiratory tract obstruction are
the key nurse steps. In the meantime, to change the
histanoxia by applying the diutretics, control the
pulmonary edema and encephaledema, use the antibiotic
to treat secondary infection are also the effective
measures. The author thinks it is important to use HBO at
early stage and thoughtful nurse in treating H2S
poisoning.
K61
The reasons and how to prevent barotraumas of
middle ear to the sudden deafness patients during
hyperbaric oxygen treatment
Lijuan Chen, Yan Jiang, Rongzhen Wang, Xiaohong Ding
The First Affiliated Hospital, College of Medicine, Zhejiang
University, China
Object: To investigate how to prevent barotraumas of
middle ear during hyperbaric oxygen (HBO) treatment to
the sudden deafness patients. Methods: There were
1019 patients received HBO treatment in our hospital
during Jan-04 to Dec-07 and 68 patients among them
encountered barotraumas of middle ear. The 68 patients
were divided into groups according to different reasons of
barotraumas of middle ear, first or non-first time treatment
and different educational background. And comparison
were in progress inter-groups. Results: 6.64% of all 1019
patients encountered barotraumas of middle ear. Among
them 87% (59 patients) were mild, 11% (7 patients) were
middling, 2% (1 patient) was severe. The reasons of
barotraumas
were
eustachian
tube
obstruct,
inappropriately pressure adjust and faulty operation.
There was significant difference in composition of these
three reasons. The incidence rate of barotraumas was
5.1% in first time treatment patients, and was 1.6% in
non-first time treatment patients. The difference was
significant (P<0.01). There was also significant difference
in different educational background patients (P<0.01).
Conclusion: Many factors can cause barotraumas of
middle ear during HBO treatment to the sudden deafness
patients. So the operator must pay attention to the
complication and take some things to reduce the
incidence rate of barotraumas of middle ear.
K62
Effect of different initiation time of hyperbaric
oxygenation therapy on MCAO rats with learning and
memory deficits
Hao Chen, Zhumei Bi, Ning Cui, Handong Liu
Department of Hyperbaric Oxygenation Treatment,
Shandong Provincial Hospital, Jinan, Shandong, China
Objective: To investigate the effect of initiation time of
hyperbaric oxygenation (HBO) therapy on spatial learning
and memory deficits of MCAO (middle cerebral artery
occlusion) model rats. Method: MCAO rats model was
induced. HBO treatment was administered at 6 or 24
hours after reperfusion respectively in earlier (EHBOT) or
delayed therapy (DHBOT) groups. The spatial learning
and memory abilities were evaluated by Morris water
maze test. The infarct volume ratio of each group was
measured by 2, 3, 5-triphenyltetrazolium chloride (TTC)
staining. Result: In EHBOT and DHBOT group, compared
with that in ischemia-control group, the learning and
memory abilities improved and the brain infarct volume
ratio diminished markedly (all P<0.01). Conclusion: Both
earlier and delayed HBO therapy can improve the learning
and memory dysfunction of rats with ischemia-reperfusion
brain injury. Hyperbaric oxygenation therapy shows neural
protective effect in ischemia-reperfusion injury.
K63
Hyperbaric oxygen and rehabilitative therapy on
severe traumatic brain injury 196 cases
Huiping Peng, Xiaoxin Lu, Yongjian Tang, Weihong Fang
Fuzhou General Hospital, Fuzhou, China
Objective: To investigate the effective usage of
hyperbaric oxygen and rehabilitative therapy on severe
traumatic brain injury. Methods: From March 2007 to April
2008, 196 patients of severe traumatic brain injury were
treatment
by hyperbaric
oxygen
treatment
and
rehabilitative therapy in our department, male 119, female
77, average 30.6±8.7years. All patients were severe
traumatic brain injury, treated by hyperbaric oxygen
treatment (HBOT) and rehabilitative therapy. HBOT
pressure was 1.6-0.2Mpa, patients inhale oxygen with
face mask for 60 minutes, A 5-minute break was taken to
breath air inner cabin. HBOT was performed once a day,
for 10 days as a treatment course. AT the meantime,
rehabilitative therapy was performed once or two a day for
every patient, the treatment time was 30-60 minutes,
including arthrosis passivity movement, nerve muscle
electricity stimulate, physics therapeutics, biofeedback.
Result: The number of times of hyperbaric oxygen and
rehabilitative therapy on patients was 20±4.5; the healing
rate was 85.2% (167/196); the effective rate was 14.8
%( 29/196).There were no anyone break off treatment for
unwell. Conclusion: Hyperbaric oxygen treatment and
rehabilitative therapy have significantly effect on severe
brain trauma patients, they are safety and useful.
Mechanism of ischemic tolerance induced by
hyperbaric
oxygen
preconditioning
involves
up-regulation of hypoxia-inducible factor-1α and
erythropoietin in rats
1
1
1
YunPing Li ,
ZaoYun Peng ,
Gu Guo
Jun ,
1
1
1
1
JiaJun Xu , ZhiMin Kang , WeiGang Xu , HengYi Tao ,
2
2
1
Robert Ostrowski , John H Zhang , XueJun Sun
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical University, Shanghai, China
K64
The curative effect of Hyperbaric Oxygenation
treatment child brain damage and the influence of
nursing
Jian Bao, Dao-dong Liu
The people’s hospital of Lu’an city, Anhui province, Lu’an,
China
Objective: We studied the effect of hyperbaric oxygen
(HBO) preconditioning on the molecular mechanisms of
neuroprotection in a rat focal cerebral ischemic model.
Methods: Seventy two male Sprague–Dawley rats were
pretreated with HBO (100% O2, 2 atmospheres absolute,
1 hour once every other day for five sessions) or with
room air. In experiment 1, HBO preconditioned rats and
matched room air controls were subjected to focal
cerebral ischemia or sham surgery. Post-injury motor
parameters and infarction volumes of HBO preconditioned
rats were compared with those of controls. In experiment
2, HBO preconditioned rats and matched room air
controls were killed at different time points. Brain levels of
hypoxia-inducible factor 1a(HIF-1a) and its downstream
target gene erythropoietin (EPO) analyzed by Western
blotting and RT-PCR as well as HIF-1α DNA-binding and
transcriptional activities were determined in the ipsilateral
hemisphere. Results: HBO induced a marked increase in
the protein expressions of HIF-1α and EPO and the
activity of HIF-1a, as well as the expression of EPO
mRNA. HBO preconditioning dramatically improved the
neurobehavioral outcome at all time points (3.0±2.1 vs
5.6±1.5 at 4 h, 5.0±1.8 vs 8.8±1.4 at 8 h, 6.4±1.8 vs
9.7±1.3 at 24 h, P<0.01 respectively) and reduced
infarction volumes (20.7±4.5% vs 12.5±3.6%, TTC
staining) after cerebral ischemia. Conclusion: This
observation indicates that the neuroprotection induced by
HBO-preconditioning may be mediated by an upregulation
of HIF-1a and its target gene EPO.
Purpose: Discussion the curative effect of Hyperbaric
Oxygenation treatment child brain damage and the
influence of nursing. Method: In random take out 82
examples as observation group (n=82) from hospitalized
child patient (October 2002 to October 2007), and take
the same period 78 examples as compare group. The
observation group was given conventional treatment add
HBO treatment, the compare group treat only given
conventional treatment. Observe the difference of curative
effect in two groups’ treatment the child brain damage.
Result: the cure and apparently effective rate of
observation group be higher than compare group.
Conclusion: The treatment of Hyperbaric Oxygenation
can raise the effect in child brain damage.
K65
Up-regulated HIF-1α is involved in the hypoxic
tolerance
induced
by
hyperbaric
oxygen
preconditioning
1
2
1
1
Zhaoyun Peng , ping Ren , Zhimin Kang , Jing Du ,
1
1
1
1
Qinglin Lian , Yun Liu John H Zhang , Xuejun Sun
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical, Shanghai, China
Objective: the present study was to examine the hypoxic
tolerance induced by HBO-PC and to explore the role of
hypoxia-inducible factor-1alpha (HIF-1alpha) in a global
hypoxia model. Methods: Male mice received HBO-PC
before hypoxia exposure and swimming. Results:
HBO-PC significantly prolonged the survival time and the
tolerance time of swimming under normobaric hypoxia.
HBO-PC increased the protein content of HIF-1α and
erythropoietin (EPO) in the cerebral cortex and
hippocampus and prevented the changes of blood brain
barrier (BBB) permeability and brain edema caused by
hypoxia exposure. Conclusion: The results suggested
that HBO-PC induced hypoxic tolerance in mice via
up-regulating of HIF-1α and its downstream genes.
K66
K67
Hyperbaric oxygen preconditioning attenuates motor
neuron apoptosis after spinal cord ishchemia in rats:
role of nitric oxide
1
2
1
2
Liping Wang , Xuejun Sun , Wenxian Li , Zhimin Kang ,
2
2
3
Hengyi Tao , Weigang Xu , John H Zhang
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical, Shanghai, China
Hyperbaric oxygen (HBO) preconditioning confers
neuroprotection against subsequent ischemic insult by
inhibition to neuronal apoptosis. In this study, we
investigated whether nitric oxide (NO) was involved in
HBO-induced ischemic tolerance and the underlying
mechanisms in a rat model of spinal cord
ischemia-reperfusion. HBO preconditioning, which
significantly increased NO levels in spinal cord tissue, was
produced by consecutive four times of 1-h HBO exposure
(2.5ATA, 100%O2) at an interval of 12 h. At 24 h after the
last pretreatment, rats underwent spinal cord ischemia for
9 min. Function recovery was evaluated by 15-point scale,
and motor neuronal apoptosis was detected by TUNEL
staining. Behavioral function was remarkably preserved
and motor neuronal apoptosis was notably attenuated by
HBO preconditioning (P<0.01). Intraperitoneal injection of
L-nitroarginine-methy-ester (L-NAME, 10 mg/kg), a
nonselective nitric oxide synthase (NOS) inhibitor at 20
min prior to each HBO preconditioning, abolished the
beneficial effects of HBO preconditioning. The activities of
caspase-3, -9, -8 and the expressions of mitochondrial
passway anti-apoptotic mediators in the ischemic spinal
lumbosacral tissue at 6, 12 and 24 h after reperfusion
were evaluated in HBO preconditioned rats with or without
L-NAME. NO protected neurons from apoptosis through
attenuation of cytochrome c release and caspases
cleavage along with upregulation of Bcl-2 and antioxidant
enzymes.
Intraperitoneal
administration
of thiol
antioxidant N-acetyl-L-cysteine (NAC, 200 mg/kg) at 15
min before each HBO preconditioning also blocked the
neuroprective effects of HBO by decreasing NO
production. The abovementioned data indicate that NO
produced by HBO may contribute to neuroprotection
during the preconditioning.
K68
When high pressure oxygen treatment prevents
nursing experience which the middle ear gas crushes
Gao Xiudong
Victory oil field central hospital high pressure oxygen
branch of Dongying, Shandong camp, China
Objective: Discussion high pressure oxygen branch
nursing to prevents the middle ear gas to crush the
morbidity the influence. Methods: We carry on to each
patient carefully nurse comprehensively, then, the careful
observation, the record, summarizes, carries on the
analysis. Results: Nurses earnestly after us, the patient
middle ear gas crushes the disease incidence rate
reduces obviously. Conclusion: When high pressure
oxygen treatment, carries on carefully nurses
comprehensively, but effectively reduces the disease
incidence rate which the middle ear gas crushes.
K69
Effect of hyperbaric oxygen on the rehabilitation of
neurological function impairment and Ability of
activity of daily living in patients with intracranial
aneurysms after operation
Dongjuan Li, Jinlong Liu, Xiaoning Si
Department of Hyperbaric oxygen, The First Affiliated
Hospital of Sun Yat-Sen University, Guangzhou, China
Objective: To observe the influence of hyperbaric oxygen
(HBO) combined with physical therapy and acupuncture
on neurological function impairment (NFI) and ADL in
patient with intracranal aneurysms. Methods: 80 cases
with intracranial aneurysms were randomly divided into
two groups treated with and without HBO therapy besides
conventional treatment. The score of NFI and Barthel
were evaluated before and after treatment. Results: The
score of NFI after treatment was lower than before
treatment in two groups. It was lower in HBO group than
in control group after treatment. The score of ADL after
treatment was higher significantly as compared with
before treatment in HBO group and control group. After
HBO therapy they were higher than those in control group.
Clinical effect wasassociative with the day of beginning
and Hunt-Hess grading system. Conclusions: HBO
therapy and other rehabilitative training were actively
used in patients of intracranial aneurysms in the early
stage after operation, could improve neurological function
and ADL and quality of life significantly.
K70
Effect of hyperbaric oxygen therapy in ischemic
stroke：
：150 cases report
Li Li, Jiali Zhang, Yue Yao, Huichun Zheng, Xiaoqin Du
HBOT Center of Southwest Hospital, Third Military
Medical University, Chongqing, China
Objective: Observe the effect of hyperbaric oxygen
therapy (HBOT) in patients with ischemic brain stroke.
Methods: 150 cases of brain infarction patients, which
performed HBOT, were analyzed retrospectively. Results:
The symptoms of 54.0% patients with HBOT were
improved significantly, but it is 44.0% in another 150
cases of brain infarction patients without HBOT during the
same period. If HBOT could be initiated in 30 days after
attacked, the ratio of patients with significant symptoms
improvement is 69.7%, 57.5% in 31-60 days, higher than
in 60-90 days (40.0%) and after 90 days (23.5%). The
best outcome was occurred in patients with HBOT course
of 21-30 days, 63.6% of which achieved significant
improvement, followed by11-20 days (59.7%), less than
10 days (52.3%) and more than 30 days (31.8%).
Conclusions: HBOT can do benefit to patients with brain
infarction and it is beneficial to initiate HBOT as early as
possible, and the course of HBOT should better not less
than 20 days.
K71
The use of Hyperbaric oxygen in infants external
hydrocephalus comprehensive treatment
Wu Xiuyun
Department of Hyperbaric oxygen Taihe Hospital of
Yunyang Medical College, Shiyan, Hubei, China
Objectives: To investigate the role of hyperbaric oxygen
treatment in infants and young children of the
hydrocephalus and analysis the leading risk factors of
external hydrocephalus. Methods: 100 cases of the
children with hydrocephalus using Hyperbaric oxygen +
nerve-brain-training physical rehabilitation treatment,
infants with single oxygen chamber therapy a day, 12
days for a course of treatment, intermittent 20 days , A
total of 2 to 3 course. Treatment of pressure 0 to-4months
1.6 ATA; 4-8months 1.7 ATA; 8-12months1.8 ATA; clinical
follow-up from 6 months to 1 year. Results: 100 cases of
children with the 2 to 3 course of treatment, clinical
symptoms disappear, six months of follow-up review of 74
cases of head CT subarachnoid fluid decreased to normal,
one-year follow-up of 26 cases of non-developmental
disorders and nervous system abnormalities, The total
effective rate was 90%. The risk factors of external
hydrocephalus is the lack of oxygen in perinata,
suffocation, encephalitis, trauma, such as the factors that
injury the central nervous system. Conclusions: The
external hydrocephalus children in the comprehensive
treatment on the basis of early supplemented with
high-pressure oxygen treatment, clinical prognosis is
good for children with neural, mental development play a
positive role in promoting.
K72
The Nursing during the hyperbaric oxygen treatment
after Skin grafting
Wang Wenjie
Tai He Hospital affiliated to Yunyang Medical College,
Shiyan, Hubei, China
Objective: Explore the relationship between the success
rate of skin after transplantation and the nursing during
the hyperbaric oxygen treatment after skin grafting.
Methods: 40 patients were studied who took the
reconstruction of skin grafting because of the trauma or
the organizations after surgeries in our hospital since
2005. Conventional treatment, nursing and the hyperbaric
oxygen treatment were carried out at the same time.
single pure oxygen chamber treatment pressure 0.2 Mpa,
or, decompression of the 25min, Regulators 40 min, in a
small cabin air pressure, the pressure of 0.2 Mpa, oxygen
60 min (30'2 +10'), or, decompression of the 25 min.
Results: The efficacy is closely related to the time
between the transplant and the hyperbaric oxygen
treatment. The time is shorter and the effect is more
obvious. And the Special nursing during the Hyperbaric
oxygen treatment has played a positive role in promoting
and prevented the incidence of complications, the
effective rate was 90%. Conclusions: The Hyperbaric
oxygen treatment has a significant effect, and should be
used earlier during the conventional treatment.
K73
Significance of vascular endothelial growth factor in
steroid-induced vascular necrosis of femoral head
treated with hyperbaric oxygen
Cui Cao
Department of Orthopedics, Taihe Hospital of Yunyang
Medical College, Shiyan, Hubei, China
Objective: To investigate the stage-changes of vascular
endothelial growth factor (VEGF) in the mode of
steroid-induced avascular necrosis of femoral head and
after treated with hyperbaric oxygen, and explore the
pathogenesis. Methods: 60 adult Japanese rabbits were
randomly divided into two groups: experiment group (42)
and control group (18). As for experiment group, each one
was two times per week, and as for control group, each
one with 2ml of salt water, the experiment lasted for six
weeks. Then, rabbits in experimental group were
randomized to hyperbaric oxygen (HBO) group(16) and
control group(16). The HBO group receive HBO treatment,
but the control group was placed in the fresh air. At the
end of 2, 4, 6, 8, 10, 12 weeks, the ratio of VEGF,
pathological changes of femoral head in light microscope
and electron nicroscope, imaging changes were observed.
th
Results: At the end of 6 week, a propotion of osteocytes
decreased in volume and the nuclei shrunk. Many
osteocytes underwent necrosis, dissolved and became
fragments. The masculine expression of VEGF was slight.
th
At the end of 12 week, scattered osteoblast cells could
be observed around the bone trabecula. Nascent bone
cells and collagenous fibri could be found. The ratio of
masculine cells of VEGF, which were mainly in the
endangium, was increased obviously. At the same time,
there were many newborn micrangium. Conclusion:
HBO can accelerate the vascular developing and
ossification by promoting the exudation of VEGF and
improve bone repairing.
K74
Nursing methods of respiratory tract in severe
craniocerebral injury patients after tracheotomy who
were treated with high pressure oxygen and
combined therapy
Ming Huang, Jing Bian
Department of Neurosurgery, Taihe Hospital of Yunyang
Medical College, Shiyan, China
Objective: To discuss nursing method of respiratory tract
after tracheotomy in patients with severe craniocerebral
injury, who were treated with high pressure oxygen and
combined therapy, and decrease the infection rate
respiratory tract and improve the survival rate. Methods:
198 patients with severe craniocerebral injury were
treated with tracheotomy and high pressure oxygen and
combined therapy, all patients were given environmental
nursing, postural nursing, sputum nursing, sputum
culturing for germs, controlling the correct decannulation
time and so on. The data was analyzed retrospectively.
Results: All the patients were kept unobstructed of
respiratory tract. Infection rate of respiratory tract was
24.9% and survival rate was 89.2%,survival quality was
improved obviously and the rate of good was 74.5%.
Conclusion: Timely and effective respiratory tract nursing
for patients with severe craniocerebral injury who were
performed with tracheotomy can decrease complication of
respiratory tract infection effectively and make for the
rehabilitation of patients.
K75
Nursing experience of 26 cases with spinal cord
jnjury treated with hyperbaric oxygen therapy
Dongyun Zhang, Haiju Pi
Department of spinal surgery, Taihe Hospital, Yunyang
Medical College, Shiyan, Hubei, China
Objective: To investigate the therapy effect of spinal
fracture with spinal cord injury treated with spinal cord
decompression and internal fixation and hyperbaric
oxygen (HBO) therapy. Methods: From 2001 to 2003,
252 cases of spinal fracture with spinal cord injury were
treated with spinal cord decompression and AF nail
fixation in our hospital, after operations, 26 cases treated
with hyperbaric oxygen (HBO) therapy, and the other 26
cases treated with routine therapy. The therapy effect was
observed according to Frankel standard, and the further
low back pain was also observed, the follow up period’s
was one year. Results: The 26 cases treated with
hyperbaric oxygen achieved better treatment effect than
the other 26 cases. Conclusion: Spinal fracture with
spinal cord injury treated with hyperbaric oxygen therapy
can acquire more better treatment effect than treated only
with operation.
K76
Hyperbaric oxygen treating and caring 48 cases of
severe brain injury and tracheotomy patients
Jing Bian, HouCheng Li
Taihe Hospital, Yunyang Medical College, Shiyan, Hubei,
China
Objective: To explore the nursing experience of
tracheotomy and severe brain injury patients during
hyperbaric oxygen therapy. Methods: As the 48 cases of
severe brain injury and tracheotomy patients who
admitted in our hospital in 2005 for the study, during the
conventional surgical treatment and care, recovery
supplemented by hyperbaric oxygen treatment, a single
pure oxygen chamber treatment, pressure 0.1 Mpa,
respectively increased pressure and decompression 25
minutes , maintain pressure 40 min, a small,
air-pressurized module, the treatment pressure is 0.1 Mpa,
oxygen 60 min, (30 * 2 +10), or, respectively increased
pressure and decompression 25 minutes. Results: on the
basis of clinical treatment, after vital signs Stability, no
further bleeding tendency, as soon as possible to
hyperbaric oxygen therapy, to promote the lives of
comaawakening and improve the life function. Of 48
cases patients, cured 21 cases, markedly effective 13
cases, improvement 9 cases, 2 cases of invalid, 2 cases
died, the total efficiency is 91.66 percent. Conclusions:
as soon as possible safe and effective for hyperbaric
oxygen treatment, while the care measures before and
after hyperbaric oxygen treatment is particularly important,
and actively deal with complications, can improve the
prognosis of severe brain injury, has made significant
effect.
K77
Hyperbaric oxygen treatment and rehabilitation
training in the United Feng-chi point of acupuncture
Pseudobulbar palsy analysis of the clinical results
HouCheng Li, HongMei Zhang, GuangJian Liu, JunHua
Wang, ZhongMing Rao, Ming Zhu
Tai He Hospital affiliated to Yunyang Medical College,
Shiyan, Hubei, China
Objective: To evaluate the clinical effects of hyperbaric
oxygen treatment and rehabilitation training in the United
Feng-chi point acupuncture treatment after a stroke
pseudobulbar palsy. Methods: 90 patients were randomly
divided into I (ABC). II (ABC) group, were given
conventional treatment of cerebral vascular disease, and
another to give I (B), II(B) group hyperbaric oxygen
therapy, I (C), II (C) Group and swallowing function of
rehabilitation training; II(ABC ) Group Feng-chi point of
acupuncture treatment; to their own improved wa tian
surname swallowing function classification standards in
the treatment of various points in time before and after to
evaluate the patients with swallowing function. Results: In
addition to I A group, the score of the other five groups in
eight points in time have significant differences (P <0.05,
or P <0.001), the score differences between group I and II,
therapy group in seven points in time had statistical
Significance (P <=0.001); In addition to the three days
treatment, I, II group at other times, the group score
differences were statistically significant (P <0.05, or P
<0.001). Conclusions: Comparison of clinical, hyperbaric
oxygen treatment, more effective than conventional
therapy group, rehabilitation training group is better than
hyperbaric oxygen treatment group, the Joint Feng-chi
point acupuncture treatment group is better than
non-acupuncture treatment group.
K78
Hyperbaric oxygen therapy for brain tumors, cerebral
vascular tumor after clinical effectiveness analysis
Houcheng Li, Ming Huang, Jing Bian, HongPing Ning, Xiu
Yun Wu, Ming Zhu
Shiyan Yunyang Medical College, Taihe, Hubei, China
Objective: To analyze the clinical effect of Hyperbaric
oxygen treatment of brain tumors, cerebral vascular tumor.
Methods: Choose 96 patients of brain tumor, cerebral
vascular tumor who have surgery Since December 2000
in our hospital's neurosurgical hospital, were randomly
divided into two groups. 48 cases of hyperbaric oxygen in
the conventional drug therapy group at the same time,
supplemented by hyperbaric oxygen treatment. Of which
18 cases were single pure oxygen chamber treatment,
pressure 0.2 Mpa, Regulators 40 min, upward and
downward pressure each 25 minutes. 20 patients with
small cabin air pressure, pressure 0.2 Mpa, oxygen 60
min (30 '× 2 +10'), the middle rest 10 min, upward and
downward pressure each 25minutes. Once a day, 12
times for treatment. For the longest time, 68 times, the
shortest are six times. The control group of 48 cases,
conventional drug treatment. Results: HBO were cured
30 cases, markedly effective in 13 cases, improvement in
two cases, invalid three cases, 93% efficient. The control
group cured 16 cases, nine cases markedly effective,
improved in 10 cases, 13 cases invalid. Group 2 cure rate
and the total efficiency of the differences were statistically
significant (P <0.05). Conclusion: In the premise of drug
treatment, that the surgery patients of brain tumor, brain
tumor blood vessels, supported by hyperbaric oxygen
treatment, clinical treatment more effective than
conventional drug group, and the sooner the better
therapeutic effect.
network structure upstream activating the function of the
system, Is conducive to patients regained consciousness.
Enhance the liver and kidney function, strengthen the
poison of detoxification and excretion.
K79
Nursing of the hyperbaric oxygen treatment to the
reconstructed right hand after operation
Guiping Hu, Qiong Zhang, Yan Quan
Taihe Hospital of Yunyang Medical College, Hubei, China
K81
Study of hyperbaric oxygen therapy on patients
complicating with disordered brain function and
incision of trachea
Huijun Hu, Xiaowen Pan
Hyperbaric Oxygen Center of PLA, Naval General
Hospital, Beijing, China
Objective: To investigate the contribution of hyperbaric
oxygen treatment to operation achievement ratio of limbs
transplantation and the effect on functional rehabilitation.
Methods: A case of dumped hand, after the excision of
the left upper extremity, was heterotopic transplanted to
reconstruct the right hand. Besides the symptomatic
treatment and supportive treatment of anticoagulation,
dilat blood vessel, anti-inflammatory, analgesia. To carry
out the hyperbaric oxygen treatment 6 hour after the
operation. The pressure is 0.2Mpa(2ATA). The time of
absorption oxygen is 60 min. Intermedial recess is 10 min.
Both the process of boosting pressure and
decompressing pressure are 25 min. Deploy seven
treatments in the three days. Then one treatment per day.
In the stage of therapy after operation, the specific nursing
of keeping the affected extremity warm and raising the
affected extremity, local proper fixation are very important.
Results: The treatments promote the functional
rehabilitation of affected extremity. Half year after of the
operation, the follow up find that the recovery of the
reconstructed right hand is satisfaction. The rotation
function of the antebrachiu: pronate 70 °, supinate 70 °
the extension, flexion and prehension of fingers are
normal. And the muscle force is IV grade. Conclusion:
Prompt hyperbaric oxygen treatment and standard
specific nursing after successful operation are the
important measures that promote the functional recovery
of the implanted extremity.
K80
Hyperbaric oxygen treatment of toxic encephalopathy
tetramine case
Li Hongyan
The Peoples Hospital HBO Departments of Qitaihe city
Heilongjiang Province, China
Objective: The implementation of high-pressure oxygen
treatment, toxic encephalopathy and multiple organ failure
patients regained consciousness as soon as possible, to
language, physical function; to improve multiple organs.
Methods: Case Analysis, Hyperbaric oxygen treatment.
Results: After high-pressure oxygen treatment, the
patient awareness of recovery, normal intelligence,
language physical function returned to normal, liver and
kidney function returned to normal. Conclusion: HBO can
reduce intracranial pressure, improve cerebral edema. At
the same time increase the vertebral - basilar artery blood
flow system, thereby increasing the blood supply the brain
stem, contribute to the restoration of the brain stem
Objective：
：To study the equipments, therapy opportunity,
security and institution of hyperbaric oxygen (HBO)
therapy on patients complicating with disordered brain
function and incision of trachea. Methods：
：Retrospective
analysis was done on the patients who complicated with
disordered brain function and incision of trachea from Jan.
1999 to Jun. 2007. Their data were summarized. Firstly,
all kinds of equipments through which the patients after
tracheotomy could inhale oxygen in HBO chamber were
compared. Their structure, serviceable range, security,
efficiency of inhaling oxygen and therapeutic
efficacy were studied. So the problem on equipments for
the patients was solved. Secondly, all kinds of sputum
aspirators were compared, including their principle,
structure, serviceable request and limitations. By using
these sputum aspirators, the problem on aspirating
sputum in HBO chamber was solved. So the security of
patients was ensured. Thirdly, we proposed that the
patients after tracheotomy should be administered HBO
therapy earlier. However, the following factors must be
considered, such as the severity of primary diseases, the
severity of pulmonary infection, the type of HBO chamber,
the equipment in HBO chamber, the diathesis and
experience of medical personnel in HBO chamber. In
addition, informed consent and institution about medical
personnel in HBO chamber were very important.
Especially for the severe patients, when administered
HBO therapy, they must be accompanied by doctors or
nurses. Results：
：After the factors including equipment,
medical treatment, nursing, institution and so on had been
studied and summarized, we solved the problem that the
patients complicating with disordered brain function and
incision of trachea could be administered HBO therapy
promptly and safely. Conclusion：Safety should be the
most important premise. Besides, we should make the
best of all means to start HBO therapy as early as
possible, through which the patients complicating with
disordered brain function and incision of trachea could get
a better result.
K82
The foreground of Lidocaine treatment for DCS
Li Changchun
Navy submarine academy of PLA, Qingdao, China
With the further cognition to the DCS pathology, some
pharmacologic agents raise the people’s interest.
Lidocaine is one of the most promising pharmacologic
agents because of the obvious treatment effect.
Pathologic development of DCS: First, people
considered DCS was result from simple physical injury
of bubbles; however, following study show the pathology
is complicated. It is believed that the body’s host defenses
respond to bubbles as foreign bodies, leading to the
activation of enzymes, complement and clotting factors,
and the release of vasoactive substances .The activation
of the body’s defense system initiates a vicious cycle the
leads to multiple organ failure .To the series of
inflammation reaction, pharmacologic treatment is very
important. Pharmacology of lidocaine: Lidocaine is local
anesthesia agent,since 1963, it has been used as
antiarrhythmic agent for ventricular tachycardia and
fibrillation. It begin to work within 15-30 seconds after
intraveneous, and then rapidly distribute to heart, brain,
lung, liver etc. Lidocaine can work on heart directly, but
less effect on vegetable nerve. The present study
of lidocaine to treat DCS: It is a tendency to use the
lidocaine to treat serious DCS, these results from some
animal experiments in vivo. In 1984, Evans found
lidocaine can protect nerve system in animal experiment.
This raises people’s interests to lidocaine as adjuvant
treatment for DCS. Subsequence, many scientists also
proof this view and then study its mechanism in rat and
rabbit experiments, they found lower-dose lidocaine can
preserve conduction in isolated nerves and inhibits cation
leakage, and inhibit the adhesion of neutrophils to
vascular endothelium, but the mechanism is unknown.
However, regardless of the mechanism, lidocaine may
offer protection by preventing leukocytes from adhering to
damaged endothelium.
Presently, the Adjuvant
Treatments Committee of the Undersea and Hyperbaric
Medical Society considers the use of lidocaine to be
beneficial in cases of AGE presenting early for treatment.
K83
The near and far-period effect of hyperbaric
oxygenation on insomnia
Kuang Aihua
Cardiology Department of the Affiliated Hospital to
Ningxia Medical College, Yinchuan, Ningxia, China
Objective: To survey near and far-period healing efficacy
of hyerbaric oxygenation (HPO) in patients with insomnia.
Method: 84 patients with insomnia without organic
disease were randomly devided into drug-group and
oxygen-theapy group, and respectively were given drug
theapy and HPO theary, Record Pittsburgh Sleep Quality
Index (PSQI) of the two groups before and after treatment,
and then record and compare/analysis average time of
getting into sleeping and sleeping which is after one
month and six months treatment. Result: The effect of
HPO-theapy group obviously improve than drug-group,
the effect of after one month treatment and six months
treatment is similar. Conclusion: HPO can add to
effectiveness of the conventional drug-treatment and has
a reliable far-period effect.
K84
Observation And Nursing Intervention Of Diabetic’s
Hypoglycemia With Hyperbaric Oxygen
Kong Lei
Guangxi Zhuang Autonomous Region People’s Hospital,
Nanning, China
Objective: To probe into nursing intervention with
diabetic’s hypoglycemia with hyperbaric oxygen. Method:
The characteristics of diabetic’s hypoglycemia with
hyperbaric oxygen were analyzed retrospectively and
nursing countermeasures against it put forward. Result:
Hypoglycemia of eight diabetic cases with hyperbaric
oxygen got timely treatment, and with consequent
remission, continued to take up oxygen till they got
decompressed and discharged from the cabin; thus
ensuring the HOB treatment. Conclusion: For diabetic’s
hypoglycemia with hyperbaric oxygen, it is significant to
give proper health education, dietary guide, medication
instruction and warm-up direction. And it is necessary to
understand the patient’s blood pressure, blood sugar,
dieting, medication, metergasis of his liver and kidney in
order to act on requirements. In the HOB treatment it is
to observe strictly, check hypoglycemia and deal with it
betimes, and following discharging, it is needed to find the
cause of hypoglycemia for the purpose of avoiding
recurring of it.
K85
Clinical study of assistant treatment with hyperbaric
oxygen in reflux esophagitis
1
1,
2
1
XU Li-min , KO Lei ZHOU Qing-nan , FENG Dao-rong ,
1
FAN Qiu-ping
1. Department of Hyperbaric oxygen, People’s Hospital of
Guangxi Zhuang Autonomous Region, Nanning, China
2. Department of Gastroenterology, People’s Hospital of
Guangxi Zhuang Autonomous Region, Nanning, China
Objective: To observe the clinical effects of using
hyperbaric oxygen (HBO) to assist in treating 30 patients
with reflux esophagitis. Methods: 60 patients with reflux
esophagitis were divided averagely into the HBO group
and conventional therapy group, both groups were given
Omeprazole. Doperidone and L-Glutamine and Sodium
Gualenate Granules, while the HBO group received HBO
in addition to drugs for 8 weeks as a course. Then
compare the improvement of clinical symptom and
mucous membrane of esophagus, and compare the
variation of clinical symptom at 6 month after treatment
ending, to study the recrudescence rate. Results: The
effective rate of clinical symptom and mucous membrane
of esophagus were significantly higher inpatients of HBO
group than that in conventional therapy group (p<0.05).
Conclusion: Our data suggest that the assistant
treatment with hyperbaric oxygen in reflux esophagitis
may get better clinical results than conventional therapy
and deserve to be widely accepted.
K86
Effects of hyperbaric oxygen therapy 660 patients
with craniocerebral injury
Wenbo Jin, Min Li, Feng Xu, Ping Wan, Yusheng Ge
Department of Hyperbaric Oxygen, Yijishan Hospital,
Wannan Medical College, Wuhu, China
Clinical information: 660 patients (465men and 195
women) with craniocerebral injury from Jannary 1994 to
August
2007
were
studied.
The patients’ ages ranged from 5 to 72 year old. The
course of disease ranged from 6 to 97 days. Causes
resulting in Injury: Road accident 465, smash and hit
accident
142,
crash
accident
53.
The control group ,aged from 4 to 70 years old and with
the course of disease ranged from 8 to 85 days, was
276 craniocerebral injury patients without undergoing
hyperbaric oxygen therapy,
No
significant differences were observed in terms of Glasgow
Coma
Scale(GCS)
for the age and brain contusion
between the two groups. Methods and Results: The
pressure was 0.2Mpa
(2.0ATA)
and pure oxygen was inhaled for 30min × 2 with 10min air
break by mask. Severe cases with incision of trachea or
low spontaneously breathing undertook first-class oxygen
therapy using “humidification sterilization low-resistant
device to inhale and discharge oxygen” developed by
author. The hyperbaric oxygen therapy (HBO therapy)
was carried out once a day (special cases twice a
day), and 10 times as a course. The course of therapy
ranged from 1 to 12 courses, with the average of 2.8
courses. The two groups undertook adjunctive therapy of
dehydration, sufficient hormone, cell activator, correcting
disequilibration of water and electrolyte, enforcing function
training. The curative rates and total effective rates
of HBO group were 70.45% and 94.32% , and were
significantly higher than the control group 43.48% and
78.28% (u = 4.61,P ＜ 0.01 ). Conclusions: HBO has
become a commonly used therapeutic means for patients
with craniocerebral injury, and its curative effect has been
confirmed. Our research found that HBO therapy should
got involved in before the peak of the cerebral edema.
Severe cases with incision of trachea or low
spontaneously breathing could obtain good therapeutic
effect using “humidification sterilization low-resistant
device to inhale and discharge oxygen” developed by
author. Timely and sufficient treatment of HBO, and
personalized oxygen inhalation means are the key to
guarantee the curative effect of patiets with craniocerebral
injury.
K87
The role of hbot in regenerative medicine in anti-aging
Joseph Ting
Hi-Tech Hyperbaric Medical Centre, Kuala Lumpur,
Malaysia
Hyperbaric Oxygen Therapy (HBOT) has been proven to
be of extreme importance in the Human Embryonic
Stem-cell transplantation and Xeno-transplantation. There
are different aspects of Regenerative Medicine in
Anti-Aging. In this case the overview of future Medical
treatments relies on New Scientific approaches like
Nano-Medicine, Embryonic Stem Cell Transplantation,
Chromosomal Gene Insertions and healthy life styles in
adjunction with HBOT. Approach: The world is now
experiencing climatic changes and extreme global
warming not to mention air pollution and shortage of food
production and escalating fuel prices. These in effect have
presently reduced oxygen concentration at sea level in
comparison to past decades. This is mainly due to the
high level of carbon particulates in the air and
contamination of drinking water and lesser arable land for
farming. Consequently oxygen production level is greatly
reduced. Conclusion: Future medicine inter-links closely
with HBOT in Regenerative Anti-Aging Medicine.
K88
The high pressure oxygen exposes repeatedly to
organism function correlation influence discussion
Jianping Jiang, Yanling Wang
NO.254 Hospital of PLA, Tianjin, China
Objective: For HBO repeated exposure to some of the
relevant body to function in a study, for HBO to provide
the scientific basis for treatment. Methods:High-pressure
oxygen through clinical observation and animal
experiments on blood coagulation, the brain erythropoietin
content, liver function, brain trauma neurons ultrastructure
of the immune function and the impact analysis. Results:
Conduct clinical hyperbaric oxygen treatment indications
in the correct choice under the premise of the
implementation of rigorous scientific treatment, HBO
treatment is satisfied with the results. However, long-HBO
therapy on the bodys clotting, liver, and immune and brain
functions, such as the impact of the ultrastructure was not
obvious at present. Conclusion: How to make more safe
and effective treatment of HBO, the volume - the validity
of our research will need to resolve the issue.
K89
Effect of related factors on development and the
impact of delayed encephalopathyAfter acute Carbon
monoxide poisoning
Ji Yunping
Tianjin Peoples Hospital, Tianjin, China
CO poisoning delayed encephalopathy occurred in
patients with acute poisoning has been sober a few days
or a few months later. Objective: According by CO
poisoning patients ‘the age, the degree of intoxication,
whether complications, the first high-pressure oxygen
treatment, treatment programmes, whether cerebral
infarction history, and other relevant factors different on
the incidence of delayed encephalopathy different.
Method: I will return to the my hospital Gaoyayangke
treated in the acute Moderate and severe carbon
monoxide poisoning patients in 134 cases, both in the
high-pressure oxygen treatment before the quantitative
detection of oxygen hemoglobin > 30%.CT brain Show no
hemorrhage and lesions. In the acute phase are different
levels of coma.treatment: in a coma during the entire
process under the pressure of 0.1 Mpa oxygen absorption
2 h. twice daily.After sober changed intermittent oxygen
(30＇x2 +10＇). each Day 1. By age into three groups. A
group of 26 cases. 60-year-old. Results: 1. A group of
delayedencephalopathy in four cases (15%). B group, 10
cases of delayed encephalopathy (22%), C group, 18
cases of delayed encephalopathy (32%). two cases of
death. 2. Delayed encephalopathy occurred with the
extent of poisoning and hyperbaric oxygen treatment and
the first time and whethe complications and whether
cerebral infarction and Brain atrophy and the adequacy of
the relationship between the show: 134 cases of
recovered patients in 100 cases ,32 cases occurred late
encephalopathy, two cases of death. Delayed recovery
group Compared with that group encephalopathy ,the
degree of light poisoning recovery group, with cerebral
edema and cerebral infarction and cerebral atrophy
less ,Attending the first time 4h of the 14 cases,
high-pressure oxygen treatment time was insufficient.
Conclusion: The greater the age, the incidence of
delayed encephalopathy was higher;. level of CO
poisoning is more serious, visit the time is nighter, the
treatment time is not full, the more complications,
particularly that patients with cerebral infarction and
cerebral atrophy are the more prone to occur delayed
encephalopathy.
K90
A non-resistance absorbing oxygen access device
Mingyi Ji, Yingying Liu, Meixiu Feng
The second people hospital hyperbaric oxygen section,
Shenzhen, China
Objective: Improve the pipeline which is the second
grade of oxygen pressure reducer in order to reduce the
oxygen resistance. Methods: This device accesses
oxygen storaging devices in the original pipeline which
absorbs oxygen in the atmospheric with the second grade
pressure reducer. Results: Accessing oxygen storaging
devices almost can eliminate the additional resistance
which produced by the second pressure reducer.
Conclusion: The accessing devices fully meet the
demand that people who is the weak breathing acess
oxygen for oxygen therapy via the second grade pressure
reducer, meantime, expand the scope of services and
efficacy of the medical equipment.
K91
GCS improvement after hyperbaric oxygen therapy in
traumatic brain injury
Yue Yao, Fei Li, Mei Li, Xiaoqin Du, Hua Feng
HBOT Center of Southwest Hospital, Third Military
Medical University Chongqing, China
Objectives: To observe the effects of hyperbaric oxygen
therapy (HBOT) on Glasgow coma scale (GCS) in
patients with traumatic brain injury (TBI) and the
influences of course and initiating time of HBOT on the
therapeutic effects. Methods: 105 cases of TBI patients,
which performed HBOT more than 30 days in HBOT
Center of Southwest Hospital, were analyzed
retrospectively. The GCS improvements were compared
with 29 cases of TBI patients without HBOT during the
same period. They were also compared between patients
with different severity, initiating times and courses of
HBOT. Results: The GCS improvement of patients with
HBOT was 3.97±2.65, especially in severe TBI patients
(5.22±2.49), Both were higher than that without HBOT
(2.38±2.16) (P<0.01); The outcome was better in patients
with HBOT course over 50 days (5.03±2.95) than that less
than 50 days (3.32±2.27)(P<0.01); When HBOT could be
initiated in 30 days it was 4.79±2.64, higher than initiated
after 30 days (3.00±2.34) (P<0.01). Conclusions: HBOT
can improve GCS of patients with TBI. It is beneficial to
initiate HBOT as early as possible, and the course of
HBOT should better not less than 30 days.
K92
Hyperbaric oxygen therapy a case of cortical
blindness after coronary angiography
HouCheng Li, Yu Liu, HongMei Zhang,
XiuYun Wu, HongPing Ning, Ming Zhu
Tai He Hospital affiliated to Yunyang Medical College,
Shiyan, Hubei, China
A 65-year-old male, at 14:30 on November 27, 2007,
because of "coronary atherosclerosis sclerosis", the
femoral artery catheterization, coronary angiography
(CAG) + cerebral angiography. No appeal of discomfort,
returned to the sickroom after 1 h, the sense of dizziness,
following a looking at things fuzzy, flu-gradually
disappeared, binocular vision loss. Immediately to expand
the blood vessels and lowering blood pressure and
dealing with symptoms, rapid rehydration promote
contrast agent excretion. Condition not improved, the eye
fundus examination by urgent consultations "Bilateral
ischemic optic disc lesions,bilateral retinal artery
embolization incomplete." Immediately to hyperbaric
oxygen therapy to improve the retina and optic nerve
head ischemic changes, delaying the process of blinding.
Treatment of pressure 0.2 Mpa (2ATA), oxygen absorption
60 min (30 〃 × 2 +10 〃), the middle rest 10 min. The
second time after treatment in patients with eyes bright
sense of self, in the third treatment, her eyes turning to the
people, can clear five fingers.through 20 treatments,
binocular vision for the inveatigation Vos1.2, Vod1.0,cured
and discharged from hospital. HBO can quickly increase
blood oxygen, extend the diffusion distance of the oxygen
in the organization , quickly improve and rectify the the
hypoxic state of eyes fundus organizations , rapid
restoration of visual function, and saved the eyesight of
the eyes.
K93
The Summary of Sudden Deafness Treated By High
Baric Oxygen
Zhang Denghua
Affiliated Hospital of Inner Mongolia Medical College,
Huhehaote, China
Objective: To understand the present condition of
sudden deafness use of high baric oxygen in our
country.And all treatment method. Evaluate its result.
Method: Collection 200 articles (1999 -2008) about high
baric oxygen treatment sudden deafness with computer In
the period of january in. Reading about it, understand the
method of high baric oxygen treatment sudden
deafness.and revalue its quantity. Result: Introduced the
new exhibition in recent 2 years: The sudden deafness is
near concern to the hemal supply. high baric oxygen
treatment can recover the cell function, and can be used
as the first choice. The result is better after a week falling
ill. Establish an animal model for suddcn-onsct
sensorincural hearing loss(SSNH L). Administration of
3-NP the inner ear through the round window membrane
can reproduce the clinical manifestation of SSNHL, and
spiral ganglion damage is most prominent pathological
change in this model. Emphasize the result of 3 thesises
that announce recent 2 years, in those thesises, research
objects are the patients each from of author’s hospital.
The group of drugs to be for constract and the group of
high baric oxygen and drugs to be for exprement. They
use the standard diagnosis and treatment .And use to
statistics . All the conclusions are positiv. Treating drugs
choose from 200 thesises. 45kind drugs are divided into 5
types: The drugs of blood vessel expansion are 25 kind;
The drugs lowering the blood’s glue are 6 kind; Other are
15 kind.Special treatments are 12 kinds. Conclusion:
The data is dependable in 3 thesises; the case's number
is enough, the method that handles the data is right. The
conclusion is dependable therefore.It is importance for
group of constract.The research using single drug is more
reasonable than reunite of treatment. To choos the best
method is one mission in the future.
K94
Clinical Research on Treating Diabetic Foot with
Hyperbaric Oxygen Combined with 654-2
Yang Jinhua
the first Hospital of Shenyang, Liaoning, China
Objective: To evaluate the curative effect of hyperbaric
oxygen combined with 654-2 on diabetic foot and discuss
the new effective therapy for treating diabetic foot.
Methods: Divide349 cases of diabetic foot patients into
treatment group and hyperbaric oxygen group,654-2
group and normal treatment group. The treatment group is
treated with hyperbaric oxygen combined with 654-2 and
compared with other groups. Results: The curative effect
and examination index of the group treated by hyperbaric
oxygen combined with 654-2 are significantly superior to
that of other groups (p ﹤ 0.01) . cure rate and whole
effective rate distinguish to 69.64%, 100%, 34.09%,
80.68%, 30.16%, 75.35%, 24.42%, 40.7%, Conclusion:
The therapy of hyperbaric oxygen combined with 654-2
has distinct effect on treating diabetic foot, no reports
regarding this new therapy have been published in our
country or foreign countries. Therefore, further research,
promotion and application should be made for the
therapy.
K95
The change and significance on the contents of
serum interrelated kinases in patients with sever
acute CO poisoning during the time of hyperbaric
oxygen in treating myocardial damage
Hongtu Zhou, Jianguo Yuan, Xuechun Zhang
The Affiliated Hospital of Jiangsu University, Nanjing,
China
Objective: To observe the change of serum interrelated
kinases in patients with severe acute carbon monoxide
poisoning (ACOP) during the time of hyperbaric oxygen
(HBO) treatment. To investigate the mechanism and the
effect of HBO in treating myocardial damage. To probe
the regular pattern of creatine kinase(CK),and its
isoenzyme(CK—MB) during the time of HBO and to
evaluate the therapeutic effect of HBO. Methods: The
consciousness of 32 patients with severe ACOP was
assessed with Glasgow scale 3-7,immediately after the
1,3,5,10st time HBO therapy. At the same time, these
patients had a ECG examination. There were another 30
healthy volunteers as normal controls, the contents of
serum kinases were measured. Results: With the HBO
treatment time goes up, the patients’ ECG return to
normal and the contents of serum CK,CK-MB were
significant
decrease(p<0.01).
Conclusion:
The
myocardial damage of patients with severe ACOP can
return to normal with HBO treatment.
K96
Effects of hyperbaric oxygen on idiopathic sudden
sensorineural hearing loss complicated by vertebral
artery type of cervical spondylosis
Hongtu Zhou, Xiaoping Guo, Wenjie Tan, Jiangguo Yuan,
Xuechun Zhang
Affiliated Hospital of Jiangsu University, Nanjing, China
Objective: To investigate the clinical effect of the
synthetic treatment of hyperbaric oxygen (HBO) by
vertebral artery type of cervical spondylosis (VA-CS)
resulted in idiopathic sudden sensorineural hearing loss
(ISSNHL). Methods: 105 ISSNHL patients with VA-CS
resulted in vertebrobasilar arterial insufficiency (VAI)
weredivided into two groups, the firstgroup includes 59
patients of treatment of HBO and drug is contrast group,
the second group includes 46 patients of treatment of
HBO and cervical vertebra traction is HBO colligation
treatment group. Results: The total effective rate of two
groups are 91.5% and 97.8% (P﹥0.05) . The cure rate of
two groups are 30.5% and 62.5% (P <0.01). Half year
later, the recurrence rate of follow-up patients of two
groups are 33.3% and 6.7% (P<0.05). Conclusions:
HBO is one of effective methods to treat ISSNHL. HBO
colligation treatment can increase the cure rate and
decrease the recurrence rate.
K97
Effects of cervical vertebra traction combined
hyperbaric oxygen therapy on vertebral artery type of
cervical spondylosis
Hongtu Zhou, Wenjie Tan, Jianguo Yuan, Xuechun Zhan
g, Tiecheng Shen
Affiliated Hospital of Jiangsu University, Nanjing, China
Objective: To investigate the clinical effect of hyperbaric
oxygen (HBO) combined cervical vertebra traction
treatment on vertebral artery type of cervical spondylosis
(VA-CS). Methods: 168 patients with VA-CS weredivided
randomlyinto three groups: cervical vertebra traction
treatment group (group A), 62 cases; HBO treatment
group (group B), 48 cases; and cervical vertebra traction
combined HBO treatment group (group C), 58 cases. The
patients in each group were treated according to
designated protocols, and the therapeutic effects were
compared among the groups. Results: The total effective
rates of three groups were 80.6%, 93.8% and 97.8%
respectively. The effective rate of group C was
significantly
better
than
that
of
group
A
(PPP<0.01). Conclusions: It is suggested that cervical
vertebra traction combinedHBO therapy is an effective
method for treatment of VA-CS. The general rehabilitation
care can increase the cure rate and decrease the
recurrence rate.
K98
The analysis of clinical effect to hyperbaric oxygen
treatment on 23 cases of acute osteofascial
cornpartment syndrome．
．
Zhou Dun
First Affiliated Hospital to Liaoning Medical University,
Shenyang, China
Objective: To analysis the clinical effect of the hyperbaric
oxygen (HBO) treatment of acute osteofascial
compartment syndmne (OFCS). Methods: 23 cases were
recepted the HBO therapy. Results: The treatment effect
was positive correlation with starting time ,the effect of
starting treatment within 24 hour was better then beyond
24 hour(P<0.05). Conclusions: Some of cases, which
had been treated by HBO therapy in time were avoided
the fascial relief incision or amputation with satisfactory
results.
K99
Therapy on injury of brain by HBO together with
acupuncture & healing course
Ming Zhong, Xueqing Wang, Ping Li
The Fifth Affiliated Hospital of Sun Yat-sen University,
Guangzhou
Objective: Figure out the clinical effect of therapy on
injury of brain by HBO together with acupuncture &
healing course. Methods: 48 cases of brain injury to
patients were randomly divided into two groups with 22 in
group A which was for comparison and 26 in group B
which was for observation. The group A received
high-pressure oxygen and drug treatment only, the Group
B got acupuncture treatment and healing course besides
the treatment of the group A. Inducted the Barthel index
(MBI) to assess the basic ability in normal life as well as
neurological defects and score Fugl-Meyer motor function
score (MFA) before and after these four weeks. Results:
After four weeks of treatment, MBI, neurological
impairment and FMA of both two groups have improved,
with significant differences (P <0.01). Compared group A
with group B before and after four weeks, MBI,
neurological deficit and physically FMA had a significant
difference (P <0.05). After three weeks of treatment, total
efficiency of group B was 92.3 percent, and that of group
A was 81.8 percent, respectively, had a significant
difference (P <0.05), the group B was more effective than
the group A. Conclusion: It is worth further study in
clinical application of therapy on injury of brain by HBO
together with acupuncture & healing course basing on
comprehensive analysis on the significant positive effect
of neurological deficit, motor function and basic ability in
normal life after this combined therapy.
K100
The clinical observation and the experimental study
of the effect of hyperbaric oxygen on peripheral blood
stem cell
1
2
1
Chenggang Zheng ,
Yajing Zhu ,
Xiaohua Hang ,
1
1
1
Yafei Pang , Hongjie Yi , Qingle Liu
1. Hyperbaric Oxygen Medical Center, The Faculty of
Naval Medicine, Second Military Medical University,
Shanghia, China
2. Shanghai Navy 411 Hospital, Shanghai, China
Objective: To explore the effect of hyperbaric oxygen on
peripheral blood stem cells in Chinese people; to explore
the method in which HBO combined with colony
stimulating factor is used to mobilize peripheral blood
stem cells. Methods: 1. Employing routine hyperbaric
oxygen treatment project, we observed the level of the
+
peripheral blood CD34 cells of eight patients who had
received long-term hyperbaric oxygen treatment.We also
+
observed the level of the peripheral blood CD34 cells of
eighteen patients who received hyperbaric oxygen
treatment because of nervous system trauma, before and
after they received hyperbaric oxygen treatment. 2. Thirty
Balb/c mice were randomly and medially divided into three
groups.In one group(HBO group) mice were exposed to
HBO to mobilize peripheral blood stem cells. In another
group (HGF group) mice were injected with
granulocyte-colony stimulating factor (G-CSF) to mobilize
peripheral blood stem cells. In the other group(HBO+HGF
group) to mobilize peripheral blood stem cells mice that
were injected with granulocyte-colony stimulating factor
(G-CSF) at the same time were exposed to HBO.Before
and after mobilization the gated cell Scal-1/CD34
dual-positive population was examined by flow cytometer.
Results: 1. After seven times hyperbaric oxygen
treatment,the absolute count of the peripheral blood
+
CD34 cells of the patients elevated from (1.87±1.08)
6
6
×10 /L to (4.75±4.67) ×10 /L,2.54 times than before.After
fourteen times hyperbaric oxygen treatment,the absolute
+
count of the peripheral blood CD34 cells of the patients is
6
(2.85±1.94) ×10 /L,1.52 times than before. After twenty
times hyperbaric oxygen treatment, the absolute count of
+
the peripheral blood CD34 cells of the patients is
6
(3.26±3.49) ×10 /L, 1.74 times than before.Compared
+
with the absolute count of the peripheral blood CD34
cells of eighteen patients who did not receive hyperbaric
oxygen treatment,the absolute count of the peripheral
+
blood CD34 cells of eight patients who had received
long-term hyperbaric oxygen treatment did not show
statistics difference. 2. In HBO group after mobilization the
gated cell Scal-1/CD34 dual-positive population 2.01
times than before.In HGF group after mobilization the
gated cell Scal-1/CD34 dual-positive population 2.66
times than before. In HBO+HGF group after mobilization
the gated cell Scal-1/CD34 dual-positive population 3.76
times than before. Conclusions: Hyperbaric oxygen can
mobilize peripheral blood stem cells.After seven times,
fourteen times and twenty times hyperbaric oxygen
+
treatment, the absolute count of peripheral blood CD34
cells respectively elevates to 2.54 times, 1.52 times and
1.74 times than before.After long-term hyperbaric oxygen
+
exposure, the absolute count of peripheral blood CD34
cells does not elevate.HBO combined with hematopoietic
growth factors could mobilize peripheral blood stem cells
more efficiently.
K101
Preliminary study on intratympanic dexamethasone
injection to management of patients with profound
sudden hearing loss
1
1
1
2
Hui Zhao , Tianyu Zhang , Jianghua Jing , Jining Luo
1. ENT Hospital, Fudan University, Shanghai, China
2. Public Health College, Fudan University
Objective: to evaluate the effects of intratympanic
dexamethasone injection on patients with profound
sudden hearing loss. Materials and methods: All
patients in the present study were profound sudden
hearing loss, initial hearing loss was more than 90 dB, no
previous interventions was conducted on all patients
within 2 weeks onset of sudden hearing loss. Patients
were assigned to 3 groups according to patients’ willness,
Group
I
(local
and
general
dexamethasone
administration). 2. Group II (Intravenous dexamethasone
injection ). 3..Group III (intratympanic dexamethasone
injection). In addition, vessel dilation drugs, neurotrophy
and hyperbaric oxygen therapy also conducted on all
patients. Intratympanic dexamethasone (5mg/ml) injection
was performed during 10 days (1 injection /2 days). Pure
th
th
th
tone test was conducted on 10 , 20 , and 30 day after
intervention. Results: The factors may impact on the
prognosis were matched in all three groups. The threshold
improvement > 30 dB in group I was 81.82%, in group II
was 83.3%, in group III was 88.64%, statistical study
showed there was no significant different among 3 groups.
th
On the 30 day after intervention, pure tone threshold
improvement was 41.36 dB in group I, 43.08 dB in group II,
51.70 dB in group III. Furthermore, pure tone threshold
improvement among the 3 groups was no statistical
different. Obvious hearing improvement was noted on the
th
10 day after intervention, no further improvement
showed after 20 day intervention. More hearing
improvement was revealed in the low frequency, while in
the high frequency, less hearing improvement was
achieved. Conclusions: Comparison with intravenously
dexamethasone injection, intratympanic dexamethasone
injection did not provide more hearing improvement on
patients with profound sudden hearing loss.
K102
One-year efficacy of hyperbaric oxygen for the
patients with acute cerebral infarction
Yi Zhang, Chun-jin Gao, Huan Ge
Beijing Chao-yang hospital, Beijing, China
Objective: Observing one year to evaluate the efficacy of
hyperbaric oxygen(HBO) for the patients with acute
cerebral infarction. Methods: 192 patients with acute
cerebral infarction were divided randomly into hyperbaric
oxygen group and control group. The neurological
impairment was scored before HBO therapy and after 20
days, 3 months, 6 months and 12 months. According to
the severity of neurological impairment before therapy,
each group was divided into three subgroups. Results:
1.Slight neurological impairment patients: neurological
impairment scores of HBO groups were significantly lower
than the scores of simultaneous control groups (P<0.05).
2.
Moderate
neurological
impairment
patients:
neurological impairment scores of HBO groups after 20
days and 12 months were significantly lower than the
scores of simultaneous control groups (P<0.05). 3. Sever
neurological impairment patients: neurological impairment
scores of the HBO group after 6 months and 12 months
were significantly lower than the scores of simultaneous
control groups (P<0.05). 4. Both one-year mortality and
relapse rate were significantly lower in the HBO group
than in control group (P<0.05). Conclusion: Early stage
hyperbaric oxygen therapy can encourage neurological
function recovery in one year after acute cerebral
infarction and lower the one-year mortality and relapse
rate.
K103
Affect of the psychological intervention on parents in
lower compliance with hyperbaric
Yan ZHANG, Yun LIU, Gao-Gui HUANG
The Children’s Hospital of Kunming, Kunming, China
Objective: (HBOT) in infants and young children’s clinic.
Methods: 67 parents of lower compliance in HBOT had
psychological intervention. Measured with self-made
compliance questionnaire, to compare the changes of
before intervention and after. Results: The attitude of the
parents before the intervention: 11 cases of hesitation, 34
cases of not accepting generally, 22 cases of resolutely
leaving. The attitude after the intervention: 12 cases of
willing to accept, 13 cases of generally accepting, 14
cases of hesitation, 12 cases of resolutely leaving.
2
(X =36.83，P<0.01). According to self-made compliance
questionnaire，the score is 1.84±0.68 before intervention
and 2.90±1.44 (t=-5.4454, P<0.01) after intervention.
Conclusion: The psychological intervention on parents is
effective to patients with the lower compliance in HBOT.
To evaluate the effect of the psychological intervention on
parents in the lower compliance with hyperbaric oxygen
treatment.
K104
Repetitive hyperbaric oxygen exposures enhance
sensitivity to convulsion by upregulation of eNOS and
nNOS
Wenwu Liu, Kan Liu, Xuejun Sun
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical University, Shanghai, China
Objective: The purpose of this study was to investigate
the mechanisms in increased sensitivity to convulsions
and the role of nitric oxide (NO) and its synthases after
repetitive HBO exposures. Methods: Mice were randomly
assigned into three groups: HBO group; hyperbaric air
(HBA) group and normobaric air (NBA) group. Mice in
HBO or HBA group were exposed to hyperbaric oxygen or
hyperbaric air respectively for 60 min twice daily for 3
consecutive days (2.5 atmosphere absolute [ATA]). 24
hours after the last exposure, mice were exposed to HBO
(100% O2, 6 ATA). The latency of convulsions was
recorded. In addition, the levels of NO, NADPH
diaphorase, mRNA and protein expressions of NOS
isoforms in hypothalamus and hippocampus were
determined. Results: Latency to seizures was
significantly shortened in mice after six HBO
pre-exposures. The level of NO in hypothalamus in HBO
group was increased. The number of NADPH-d positive
cells and the levels of protein and mRNA of eNOS and
nNOS in hypothalamus and hippocampus were increased.
Conclusion: After repeated HBO exposures, elevated
NO may enhance the sensitivity to convulsions and this
may lead to seizures during the subsequent oxygen
exposures. Prevention of seizures is needed when HBO is
used as preconditioning method.
K105
Effects of early stage hyperbaric oxygen treatment on
prevention of complications in gravis type
craniocerebral injury patients
Zhang Ludi
shanghai sixth peoples hospital
Objective: to approach the effects of early stage
hyperbaric oxygen treatment (HBOT) on prevention of
complications in gravis type craniocerebral injury patients.
Methods: total 326 cases of gravis type craniocerebral
injury patients whose GCS score ≤8, among which, 244
cases were male, 82 cases were female, average age
were 36.2±6.7.after consultation of HBO doctors, those
who has indication for HBOT, and no counter indication
for HBOT, were given HBOT simultaneously with
conventional therapy, 126 cases patients who were given
the first time HBOT within 2 weeks of admission or
operation classed as early HBOT group. 108 cases
patients who were given the first time HBO treatment after
2 weeks of admission or operation classed as late HBOT
group. Among which, 92 cases patients who were not
given HBOT because human factor or economy reason
served as contrast group. three groups has no statistical
significant difference in sex, age, and GCS score, the
follow up time was within 8 weeks of admission or
operation. Results: incidence rate of pulmonary infection
of early HBOT group was significant lower thanlate HBOT
2
group X =13.78, P ＜ 0.001.late HBOT group was
significant lower thancontrast group, X =10.81, P ＜
0.005.incidence rate of gastric bleed of early HBOT group
2
was significant lower thanlate HBOT group X =7.32, P＜
0.01.late HBOT group was lower than contrast group, but
2
no significant statistical difference, X =3.31, P ＞
0.05.incidence rate of renal function lesion of early HBOT
2
was significant lower than late HBOT group, X =7.39, P＜
0.01.late HBOT group was lower than contrast group, but
2
no significant statistical difference.X =1.15,P ＞ 0.05.
Conclusions: earlier stage HBOT has significant
preventive effects on complications of pulmonary
infection, gastric bleed and renal function lesion in gravis
type craniocerebral injury patients.
2
K106
Hyperbaric oxygen the application value which
Resucitation to the cardio-pulmonary brian-A report
of 136 cases
Zhou Shurong
1st Affilitaed Hospital of Nanjing Medical Univerisity,
Nanjing, China
The hyperbaric oxygen (HBO) applies in the CPCR 136
cases, in eluding is drowing 23 cases, hanging the neck
49 cases, electric shock 3 cases, the narco-operation
accident 17 cases, Adam-stoke's syndrome 1 case, the
drug poisoning 6 cases, asphyxia 6 cases, cover-bedding
syndrome 31 cases. Of 66 cases with cardiac arrest, 42
cases were cured, notable effective (2), improvement (1),
no effect (21), and effective rate was 68.2%. Of 70 cases
with no cardiac arrest, cures 64 cases, improvement (3),
no effect (3) effective rate was 95.7%. Elaborated HBO
treatment mechanism, HBO by its unique for oxygen way
and unique mechanism, has other method of treatment
the interchange ability in CPCR finally is not the success
which the brain resucitation, regarding this HBO is playing
the important treatment role. Reiterated in the treatment
plan aspect authou before proposed about “the long
treatment course HBO therapy may expect caudes some
to consider in the past already is patient who hopeless
decerebrate condition, so long as is not the genuine brain
death, obtains the vitality” experience and idea. The
CPCR case of illness suitably in the guardianshipe
condition good, rescues in the well appointed large-scale
HBO chamber to carry on the synthesis to treat and cure,
completes accompanies the cabin work, the life symptom
changes over to the stationary phase, may treat in the
single pure oxygen cabin, must take the HBO in CPCR
application, raises one batch to be good and under the
HBO environment carrying on the rescue work the
medical troops.
K107
Research on the disinfection of the object surfaces in
which is inside of the hyperbaric oxygen chamber and
the inner surfaces of its oxygen venting tube
Zhan Hechun
Daqing Petrochemical Company employee hospital,
China
Objective: To explore an individualized omni-bearing
circulating-target technology on the disinfection of the
object surfaces of the hyperbaric oxygen chamber and the
inner surfaces of it’s oxygen delivery the tube to eliminate
the possibility of the cross-infection it has been brought by
the connection ends of the oxygen of the delivery branch
tube. Methods: To use the innovative process of
independently delivering oxygen in the multifunctional
chamber and th-30 ozone disinfection device, which is
indirectly to integrate the process disinfection technology.
Under the atmospheric pressure in which is properly close
the disinfected chamber’s door, shut off the inlet control
valve for oxygen delivery in process flow, open the
functional isolation valve of the bypass in process flow,
shut off the control valve in the process flow of oxygen
delivery, and start the ozone disinfunction device and
oxygen delivery power device. Results: This integrated
disinfection technology have reached the requirement and
purpose of the target design for both the object surface in
the chamber and the inner surface of oxygen venting
process tube. The research shows that the integrated
process disinfection technology can make unconditioned
omni-bearing disinfection to the environment inside of the
chamber and to the closed loop of oxygen venting tube,
it’s safe and reliable, has the efficacy of the individualized
disinfection, and no secondary contamination source will
be produced in the chamber after disinfection.
Conclusions: In the process flow of the oxygen directly
delivered in the chamber, and the ozone disinfection
device can not be individually used, because the process
flow has no oxygen delivery power device to be equipped,
so it’s difficult to have the function of the target disinfection
to the inner surface of the oxygen delivery tube. In this
case, although the disposable sanitary appliances for
oxygen delivery are used, the occurrence of
cross-infection still can’t be prevented effectively. Using
this technology, a new de-bottle-necking means is
equipped to the oxygen chamber project; the
unconditioned and zero-risk control of cross-infection in
the chamber is realized during the whole process of the
treatment.
K108
Evaluation of stoke with intellect and memory
curative effect
1
Xuming Zhou ,
1
1
1
1
Qun Cai , Xiuqing Li , Liyan Yan , Hong Li , DanHong So
2
ng
1. The Frist Peoples Hospital of Harbin, Ha’erbin, China
2. The second Peoples Hospital of Harbin, Ha’erbin,
China
Purpose: Detection clinic curative effect of stoke with
intellect and memory for HBO. Method: There are 2
groups 132 cases stoke of intellectual deficit and
hypomnesia. Comparison group adopt routine medication
treatment. Except HBO group routine medication
treatment under 0.25mpa absorb pure oxygen 60 mins
(break 10 mins), total 20 days, checking memory quotient
(MQ) and hastgama dementia scale (HDS) 2-group
comparison. Between 2 groups comparison show design
data of analysis of variance. Result: after 20 days
treatment, HBO group and comparison group MQ value
increased. Diversity is obviously. HBO group got score
more than comparison group. Both of them whether
before or after, diversity is significance of statistics.
Conclusion: HBO can increase dispersion and tension of
oxygen in blood which improve cerebral tissue and urge
cerebral tissue recovery. There are protection for white
matter and grey matter. After stoke intellect and memory
is obvious curative effect.
K109
Exploring on the management experience of
hyperbaric oxygen department
Shaoxia Zeng, Tao Liu, Liusi Wu, Aiqiong Feng
Department of hyperbaric oxygen, the first Peoples
hospital of Zhaoqing, Zhaoqing, China
Objective: To explore the management work of
hyperbaric oxygen department. Methods: Making and
implementing various management measures of
hyperbaric oxygen. Results: The general safety of
medical hyperbaric oxygen therapy was component
through three aspects: equipment operation safety,
operating cabin safety and therapy safety; every aspect
safety need the corresponding scientific management
measure. Conclusion: Only the equipment operation
safety, operating cabin safety and therapy safety can
ensure the successfully developing of safety work on the
department of hyperbaric oxygen.
K110
The rational selection of equipments of breathing in
oxygen on patients after Tracheotomy influencing
effectiveness of hyperbaric oxygen treatment
Yu Daoyuan
Department
of
Hyperbaric
Oxygeon,Jingzhou
Hospital,Tongji Medical College, China
Objective: To explore the rational selection of equipments
of breathing in oxygen on patients after Tracheotomy with
hyperbaric oxygen (HBO) treatment. Methods: 300
patients randomly divided into 2 groups: head cover group
(n=150) and connected tube group (n=150) were given
treatment with head cover and connected tube
equipments, respectively. 209 patients who have
successfully finished two periods of treatment been
conducted the GCS evaluations. Results: The rates of
success of finishing treatment were 99.3% in head cover
group, and 59.3% in connected tube group, respectively.
There was a significant statistical difference in the two
groups. The GCS score in connected tube group was
significantly higher than that in head cover group.
Conclusions: For the patients after Tracheotomy with
HBO treatment, the curative effect of selecting connected
tube is better than that of using head cover. They should
choose to use connected tube to absorb oxygen first, and
they may choose using head cover only if they can not
finish the treatment.
K111
Effects of early hyperbaric oxygen therapy on
function recovery in patients with incomplete cervical
spinal cord injury
Yu Youjie
Department of Hyperbaric oxygen, People’s Hospital of
Guangdong Province, Guangzhou, China
Objective: To investigate the effect of early hyperbaric
oxygen therapy on function recovery in cervical spinal
cord injury patients. Method: 72 patients were randomly
divided into early hyperbaric oxygen therapy group (E, 36
cases) and late hyperbaric oxygen therapy group (L,36
cases).The patients in both groups received the similar
rehabilitation and medicine treatment. The effects were
assessed by the American Spine Injury Association (ASIA)
motor scores, sensory scores and somatosensory evoked
potentials (SEP). Result: There was no difference
between two groups before treatment (P>0 05), and the
ASIA motor scores improved significantly after treatment
(group E P<0.001, group L P<0.05), but the scores of the
group E was significantly higher than that of the group L
(P<0.01). The ASIA sensory scores improved significantly
after treatment (group E P<0.005, group L P<0.05), but
the scores of the group E was significantly higher than
that of the group L (P<0.05). The SEP of the group E
improved significantly than that of the group L (P<0.05).
Conclusion: Hyperbaric oxygen therapy can facilitate the
function recovery, and it is better to interfere early.
K112
Curative effects of hyperbaric oxygen (HBO)
combined with manipulative therapies on limb
dysfunction after traumatic head injury
Ning Yu, Jian Lu
Department of Hyperbaric Oxygen, The Second People’s
hospital of Nangning, Guangxi, China
Objective: To investigate curative effects of hyperbaric
oxygen (HBO)combined with manipulative therapies on
limb dysfunction after traumatic head injury. Methods:
160 patients were randomly divided into two groups
according to time of admission: therapy group and control
group. The therapy group were treated with HBO and
manipulative therapies , the control group were treated
with only HBO. Fugl-Meye scores and Brunnstrom grades
were performed before treatment, as well as after 4 and 8
courses of treatment. Results: The Fugl-Meye scores and
Brunnstrom grades of therapy group are obviously higher
than that of the control group. There were significant
differences between two groups ( p<0.05.).Conclusions:
The curative effects of HBO combined with manipulative
therapies on limb dysfunction after traumatic head injury
are better.
K113
Effect of hyperbaric oxygen on the MetHb
concentration in patients with acute carbon monoxide
poisoning
1
2
3
2
Shifu Yin , Chunjin Gao , Peisong Wang , Huan Ge , Lin
2
1
1
Yang , Yongping Xie , Ruquan Jin ,
1
Jianmin Liu
1. Gaomi peoples hospital, Shandong, China
2. Beijing Chaoyang Hospital; Capital University of
Medical Science, Beijing, China
3. Department of HBO; Qingdao University Affiliated
Hospital, China
Objectives: To study the relationship between acute
carbon monoxide poisoning g and methemoglobin and the
influence of hyperbaric oxygen (HBO) on them, and to
approach the mechanism of acute carbon monoxide
poisoning. At the same time, it gives the new way to the
clinical treatment of ACOP. Methods: The ACOP group
contains 195 patients, divided into the normobaric oxygen
(NBO) group and the HBO group. The NBO group
contains 97 patients, including 66 mild poisoning patients,
21 midrange poisoning patient s and 10 severe poisoning
patients; The HBO group contains 98 patients, including
63 mild poisoning patients, 23 midrange poisoning
patients and 12 severe poisoning patients. The Normal
control group contains 191 health adults. Detect the
MetHb and COHb concentrations both before treatment
and after treatment in each group and further restriction
analysis were performed. Results: The MetHb
concentration is much higher in ACOP group than that in
normal control group (P <0.01). There is dependability
between COHb concentration and MetHb concentration in
ACOP group before treatment (R =0.418, P <0.01). The
MetHb concentration was much lower after HBO
treatment than before HBO treatment in ACOP group (P
<0.01).To mild and midrange ACOP patients, the MetHb
concentration decrease d more significantly in HBO
group than that in NBO group (P <0.05). Conclusions:
The MetHb is probably one of the most important
mechanisms to result in ACOP and makes it serious. HBO
has significant curative effect on decreasing MetHb
concentration.
K114
The nervous system of children infected with the
recovery of hyperbaric oxygen therapy clinical
observation
Yang Zhen
Tianjin Children Hospital, Tianjin, China
Objective: To observe the high-pressure oxygen
treatment on the nervous system in children infected with
the clinical significance. Method: 53 cases of the nervous
system of children infected with hyperbaric oxygen
therapy, the use of hyperbaric oxygen therapy at the
same time to give anti-infection, nerve cell activity
vasodilative drugs and anti-virus drugs symptomatic
treatment. Pressure to 0.06-0.1 Mpa, Regulators 30
minutes each for one hour, day, 10 times for treatment, a
total of three treatments, oxygen concentration in more
than 80 percent. Results: high-pressure oxygen
treatment of viral encephalitis after the cure rate is 64.10
percent with 25.64 percent efficiency of 10.25 percent
efficiency rate of 83.33 percent of cerebral infarction
recovered 16.66 percent efficient brain trauma recovery
rate was 75% 25% efficiency of high-pressure oxygen
treatment of the nervous system The total infection rate
was 97.34 percent. Conclusion: The nervous system
infection in children is common in pediatric clinical disease,
cerebral vascular disease by the virus or the disease
directly caused by brain injury with diffuse inflammation
and vascular damage, resulting in brain tissue cycle of
obstacles and edema, increased intracranial pressure So
that the brain tissue of oxygen and blood supply for the
anomaly, a serious disease can be accompanied by a
coma. Thus leading to its dominant regional campaign,
feeling the loss of function and language, intellectual
development have tremendous impact. Through
high-pressure oxygen treatment of the nervous system
infections in the clinical observation of clinical symptoms
improved significantly shorten the duration and reduce the
rate of invalidity, the resumption of intelligence, thereby
enhancing their quality of life, high-pressure oxygen can
be used as the nervous system in children infected with
an effective Treatment.
K115
8 age,male,traffic accident resulted in primary brain stem
infury. Glasgow grade 5. Revivaled after hyperbaric
oxygen treatment.
Clinic materials: 1. Case introduction: The cases
involved burn inpatients in our hospital from Mar 2006 to
Aug 2007. Among them, there are 36 male and 20 female
with the age range from 1-13 years old and average
3.6±2.8 years old. The percentage of burn area to total
area is between 10-40 and the average percentage is
15.1±6.9. They are all healthy before encountering wound
and have not aspiration injury. 2. Therapeutic methods:
The 56 patients are randomly divided into two groups: the
treatment group (A group n=28) and control group (B
group n=28). The patients of control group are treated
only by locally applying Fulikang ointment while those of
treatment group accepted the treatment of local Fulikang
ointment plus hyperbaric oxygen (HBO). 3. HBO
treatment: Patients of A group accept 100% oxygen
breathing by masks for 80min, once or twice a day. We
stimulated 10d as a therapeutic period and patients were
arranged therapy according to the healing condition of
wound surface. Normally, one or two therapeutic period
were needed. 4. Assessment Indicator: The information
involving the average healing time of wound surface,
average inpatient day, the size, thick, rigidity and color of
scar, the symptom of patients and the number of patients
who needed operation were collected as the assessment
indicator. 5. Statistical analysis: All the data were
analyzed by t test and P<0.05 was considered to be
statistically significant. Result: According to the clinic
observation, in comparison to B group, the scars of A
group were rosiness or white, soften, thin, smooth surface
and few pain and itch. And also our findings showed
accelerated healing of wound surface and reduced
average inpatient day of group A. Only 2 patients of group
A needed flap operation and have achieved the success
in the first operation, while 9 cases needed operation in B
group and among them, the operation of 2 patients were
failing. The second operation was successful under the
help of 10 times HBO therapy. Discussion: HBO has the
following therapeutic effect according to pervious study: 1)
Increasing the local blood oxygen pressure, relieving or
even rectifying tissue hypoxia, reducing edema and
improving blood flow; 2) HBO is conducive to abating flap
necrosis and enhancing flap survival; 3) In terms of scar
formation, the HBO-treated skins represent light-color,
soft texture and few pain and itch; 4) Curbing the growth
of some kinds of bacteria. To sum up, HBO is beneficial to
the healing of second-degree burn wound surface in the
respect of few usage of plasma and albumin, low
occurrence of shock and scar formation, reduced tissue
necrosism, edema, wound infection and blood poisoning,
average inpatient day and the total expense, accelerated
wound healing and operative success rate.
K116
Clinic Observation of Hyperbaric Oxygen
Promoting the Healing of Wound Surface of
Second-degree Burn Patients
1
2
X-L Yang , Y-J Deng
1. the Department of Hyperbaric Oxygen, Ganzhou
Municipal Hospital, Ganzhou, Jiangxi province, China
2. the Department of Burn, Ganzhou Municipal Hospital,
Ganzhou, Jiangxi province, China
K117
Hyperbaric oxygen treatment pontine brain contusion
and a case of dissolution of myelin
Yang Wenping
Huai’an Second People’s Hospital, Jiangsu, China
Patient: male, 52-year-old, riding on bicycle, the patient
accidentally fell down and lead to brain contusion and
Obstructive hydrocephalus. The local hospital committed
after the effects of poor drainage ventricle and
complicated by lung infection. On October 31, 2006 the
patient was transferred to our hospital. Through physical
examination, the body temperature was 37.8°,
unconscious, vertical eyes open, body activities
disappeared, mouth activities restricted, lung large
number of Romanian-moist, having heart rate, limb
extension reducing, muscle strength 0, limb tendon
hyperre flexia, Bilateral pathological levy negative, liver
damage, blood being in lower potassium, sodium, chlorine.
The right lower chest film pneumonia, head CT right
frontal lobe brain damage, hydrocephalus, low-density
pontine lesions. Through 10-days anti-infection treatment,
to dehydration, lung infection was under control, but the
nervous system symptoms had not improved. The MRI
inspection pontine basement of T1 and T2-signal, the
patient had 15-years drinking habit, he drunk about 1000
ML every day. Considering chronic alcoholism liver and
brain damage after myelin lead to the dissolution of the
CPM, the patient was immediately cured by high-pressure
oxygen treatment.0.2 ～ 0.23MPa O2 30’×2+10’. During
high-pressure oxygen treatment, first three days, twice a
day, then once a day, ten days treatment for one course.
After the first time of high-pressure oxygen treatment, the
patient was conscious and could eyes could open and
committed activities as soon as the patient went out of the
capsule. But 2 days later the patient went unconscious yet
till one week later he went back conscious again. After
15-times high-pressure oxygen treatment, the patient
could nodded to his family members. After 22-times
treatment, the patient could communicate with his family
members in simple speech removing of the trachea,
taking in flowing food; the right upper limb muscle strength
reached 1 level. Since then the treatment method was
changed into a single-capsule pure oxygen chamber.
After 4 treatment courses, four limbs muscle tension
increased and muscle strength reached 2 level and liver
function was normal. After 6 treatment courses, with the
help of others, the patient could walk by himself,
self-consumption, muscle strength reached 4 level, MRI
basically normal, after six months out of the hospital, the
patient fully recovered and could go back to work. From
this case we have made the conclusion that high-pressure
oxygen treatment can not only have the effective in the
treatment of brain injury and also have the effective for
recovery of CPM treatment. High-pressure oxygen
treatment can be used as an effective means and
recommended for promoting in clinical practice.
K118
Nursing care of cerebrovascular disease patient with
hyperbaric oxygen treatment
Yang Qunfang
Wuhan Union Hospital, Wuhan, China
Objective: To observe the nursing effect of the
cerebrovascular disease patients with treatment of
hyperbaric oxygen (HBO). Methods: 150 cases receiving
drug therapy were treated with HBO. With the measures
of comprehensive care, observing during HBO treatment
and uninterrupted treatment, so to enhance the effect.
Results: Cured 19 cases, obviously effective 45 cases,
effective 73 cases, invalid 13 cases. The total effective
rate is 91.3%. Conclusion: Under the effective nursing,
there is obvious effect of cerebrovascular disease patients
with treatment of HBO.
K119
The effect of hyperbaric oxygen treatment on the
coagulation-fibrinolytic system in the patients of
ischemic strok
1
2
1
1
Lin Yang , Wei Wang , Chunjin Gao , Baosen Pang ,
1
Zhuo Li
1. Beijing Chao Yang Hospital, Beijing, China
2. The Third Hospital of Hebei Medical University,
Shijiazhuang, China
Objective To investigate the effects of hyperbaric oxygen
treatment on the Coagulation-Fibrinolytic System in the
patients of ischemic stroke. Methods 137 patients with
acute ischemic stroke were divided randomly into routine
therapy group(routine group) ,hyperbaric oxygen
therapygroup(HBO group). There were 27 healthy
volunteers in control group (control group). We measured
the plasm concentration of AT-III, TM, uPA and uPAR
respectively in the patients with ischemic stroke on the
1stand 11thday after admission. Results Compared with
the control group , the plasm concentration of the routine
group on the 1st day, the 11th day of AT-III decreased,TM
and uPAR increased;the plasm concentration of the HBO
group on the 1st day of AT-III decreased , the plasm
concentration of TM, uPAR of the HBO group on the 1st
day, the 11th day increased (P<0.01) ,TM decreased
(P<0.05) .Conclusion In the patients of acute ischemic
stroke, hyperbaric oxygen treatment can increase the
plasm level of AT-III, and decrease the plasm level of TM
and uPAR. Thus hyperbaric oxygen treatment can
effectively improve the function of coagulation-fibrinolytic
system in the patients with ischemic stroke, and may
ameliorate therapeutic effect.
K120
The Clinical Analysis of the Hyperbaric Oxygen
Therapy in the Patients with Acute Cerebral Infarction
Lin Yang Chunjin Gao Huan Ge Lianhua Wu Liming Zhao
Beijing Chao Yang Hospital
Objective To investigate the effect of hyperbaric oxygen
therapy (HBOT) on the patients contracting acute cerebral
infarction(ACI) and to proceed the clinical analysis.
Methods Retrospective study was done in 466
hospitalized patients suffered from ACI from Jan. of 2000
to Feb. of 2005.The patients was divided into HBOT plus
drug therapy group (HBO group )：303 cases and drug
therapy group (control group )：163 cases. By using “The
European Stroke Scale” (ESS) to score the patients of
HBO group before and after HBOT and to score the
patients of control group after hospitalization and before
discharge. Then HBO group was compared with control
group. Results For the severe and the moderate ACI, the
score difference of HBO group being compared with
control group were considerably significant (P10) being
compared
with
control
group
was
significant
(P<0.05) .Conclusion Clearly HBOT had a certain effect
on severe,moderate ACI and vertebra-arteria basilaris
ACI which were better than drug therapy. The effect on
treatment times of HBOT >10 was better than that of
treatment times ≤10.
K121
Influence of hyperbaric oxygen on the value of
memory quotient and Hastgawa dementia scale in
patients with radiation encephalopathy
Yuan Jing
The people’s hospital of zhangjiajie, Hunan, China
Objective: To evaluate the effect of hyperbaric oxygen
treatment on patient with radiation encephalopathy. To
study the changes of the value memory quotient (MQ) and
Hastgawa dementia scale (HDS) in patients with radiation
encephalopathy pre-and post hyperbaric oxygen
treatment and its value. Method ： 60 patients with
radiation encephalopathy were divided into two groups
randomely, hyperbaric oxygen treatment group and
control group. The values of MQ, HDS in 60 patients were
measured pre-and post three courses of treament.
Result ： The total effective rate of hyperbaric oxygen
treatment groups was 86.6% that of control group was
60.0%, hyperbaric oxygen treatment group had a good
recovery. After three courses of treatment, the scale of
MQ, HDS was increased in both groups, but there was
significant difference between two groups (P<0.05),
hyperbaric oxygen treatment group was obvious higher
than control group. Conclusion：
：The treating effect of
hyperbaric oxygen combined with drug is much better
than that of drug only and hyperbaric oxygen can further
increase the value of MQ, HDS, HBO may be of use in the
clinical treatment of RE patients and would benefit from
further study.
carbon monoxide poisoning;
pregnant;
hypokalemia; hepatitis B virus carriers.
Glycerol fructose, Cerebrolysin Vial and hyperbaric
oxygen therapy were given. treatment plan of HBO:
pressure of 0.2 Mpa (2.0ATA), oxygen 30 min × 2 + air 10
min, time of therapy is 110 min, which boost 20 min,
deboost 20 min.in the first three days ,2 / day. Later it
changed to 1 / days. In the end it took 22 times. on May 1
the patients recovered and were discharged cesarean a
healthy boy.
2 Discussion
Whether pregnant woman after carbon monoxide
poisoning can receive hyperbaric oxygen therapy is still
remaining disputes. We believe it should be done .
The mechanism may be in:
HBO can increase the
oxygen concentration and blood oxygen tension.It can
correct hypoxia, and promote increasing of amniotic fluid,
improve foetus development and metabolism. HBO can
improve uterine blood supply and delay of blood flow so
that enhancing the mother-child functional system and
placental function. HBO promote gonadal hormones, the
formation of the placenta villi blood vessels and the
function of adrenaline. It conducive to development of the
placentai.The mother’s functions of vital organs can be
also improved. It should be noted:
hyperbaric oxygen
therapy must be done in time, combined therapy is
important. to observe the condition changes intimately.
Essential exam must be done before and after HBO
therapy and Good record must be done. B-monitoring
should be monitored.
premature babies to
high-pressure oxygen therapy can occur eyes of oxygen
poisoning. Therefore
hyperbaric oxygen therapy
treatment to late trimester of pregnancy should not be too
long and should be controlled within 1-2 courses.
K123
Comparison of therapeutic effects of HBOT on Severe
Brain Injury under 0.15MPa and 0.2MPa
Chen Jian-hui
Zhongshan City People's Hospital, Affiliated Zhongshan
Hospital
of
Zhongshan
University,
Zhongshan,
Guangdong Province, China
K122
Hyperbaric oxygen treatment in late pregnancy of
acute carbon monoxide poisoning
PENG Ya, YANG Yulan
Guilin, Guangxi NO181 People's Liberation Army Hospital
Hyperbaric Oxygen Center
March 2008, one case we treated successfully of acute
carbon monoxide poisoning in late pregnancy, are
reported as follows:
1、Case record,
F, 23, were unconsciousness after bath a day before
hospitalized. Physical exam: T:36.7 , HR:90 cpm, RR:
20 cpm, BP: 110/78 mmHg. Pregnant for 7 months.
Auxiliary examination: obstetric B-ultrasound showed:
fetal heart rate 158 cpm, good movements. ALT (26 u / l),
GOT (46 u / l), HBsAg(+),potassium (3.27 mmol / l) other
routine examinations were normal. Admission diagnosis:
Objective: To compare the efficacy of hyperbaric oxygen
at various pressure on severe brain injury，and to choose
the most reasonable protocol for this desease. Methods:
The 133 cases of severe brain injury were divided into
two groups randomly, and they were treated once every
day by the monoplace chamber. One session a day, ten
sessions for one course, all together 30 sesssions for one
patient. But the group A (67 cases) was treated at
0.15MPa for 60~70 minutes, and the group B (66 cases)
was treated at 0.2MPa for 80~90 minutes. We explored
the consciousness and reactions of these patients when
they were treated, and reexamine head CT and pass
judgment on GCS after each course of treatment is
finished. Results: There were no significant difference
between group A and group B in the duration of
unconsciousness, aphasia and Absorption of intracranial
K125
Development of a new inhalation model in hyperbaric
oxygen chamber,
An Cui, Peisong Wang
Linyi City People's Hospital, HBO department
Qingdao Medical College Hospital, HBO department
hematoma. Conclusions: There were no significant
difference in the efficacy of HBOT on severe brain injury
between the protocols at 0.15MPa and 0.2MPa. So
considering the shorter duration of treatment and the less
therapeutic cost we think the lower-pressure protocol is
better which can treat more patients with the limited
resourcs.
K124
Hyperbaric oxygen rescue a case of pulmonary
embolism
Li Lingfang
Hyperbaric oxygen chamber，Liaoning Provincial General
Hospital of Fushun Mining Bureau
A 67-year-old female Patient suddenly occured difficulty in
breathing, obvious cyanosis, pale face, mydriasis,
state-dead in the second day after cholecystectomy.
Multi-parameter monitoring showed that: BP60/40mmHg,
P139 per minute, R30 per minute, So258%.
Electrocardiogram showed: sinus tachycardia, atrial
premature, imperfect right bundle branch block. X chest
film: the thinning of the texture in the inferior lobe of
right
lung . The patient was diagnosed as acute
pulmonary embolism, the vital signs were stable after
rescue. But from December 20 to December 23, the
patient was in a coma, on ventilator to maintain vital signs
stable. The first hyperbaric oxygen treatment was given in
the first 10 days after onset. The pressure was 1.8 ATA,
15 minutes for compression, oxygen inhalation
(monoplace champer) for 50 minutes, intercalary washing
for five minutes, decompression for 20 minutes. After one
session of HBO, the patient can open her eyes by calling.
After
more than 40 times HBO, the patient was
consciousness, not only can open eyes and speak, but
also can answer simple questions.
Discussion:
HBO can decrease blood viscosity, improve ischemia in
embolized regional, and reduce pulmonary avascular
necrosis of embolism organizations, and can reduce
pulmonary edema and improve the organs of the state of
hypoxia, control the occurrence of shock, can reduce
brain swelling and the brain tissue hypoxia damage,
improve function of the network structure . The HBO
therapy promote comatose patients to regain
consciousness . The HBO therapy plays an important role
in the rescue of patients with pulmonary embolism.
The authors triturate a simple breathing patterns in HBO
champer for adults to inhale oxygen and exhale own
carbon dioxide at the same time. See attached map.
Principle: Setting that the alveolar ventilation is 350 ml.
connecting a hard hollow plastic ball , diameter 8.3 cm
and volume 300 ml(including the space between mouth
and nose mask) ,between the original oxygen masks and
Y-piece. Patients wear oxygen masks for absorption Less
than 200 kpa, at the end of each exhalation there is 300
ml alveolar gas for next inhalation within the hollow ball.
The carbon dioxide concentration of alveolar gas is 2.5
percent in the 200 kpa, the carbon dioxide concentration
of inhalation alveolar gas = 300 × 2.5% ÷ 350 = 2%. Due
to oxygen consumption, oxygen in the hollow ball is less
than 200 kpa, while hollow ball volume is less than the
volume of alveolar ventilation, so when inhale , the
oxygen through the pipeline influx into the hollow ball
beacuse of difference of partial pressure of oxygen and in
order to maintain enough alveolar ventilation, on the end
of inhale the oxygen concentration reached 98 percent.
Unless the alveolar ventilation is on big changes, we can
maintain the relative stability of the concentration of
oxygen and carbon dioxide concentration. That is, when
the oxygen content increasing, the reinhalation of carbon
dioxide can increase blood flow to further improve hypoxia.
Comparising the new and old breathing patterns by
simulation experiments, there is no obviously difference.
K126
Non-resistance oxygen therapy device in mulitplace
oxygen chamber
Yu Xuelai
department of hyperbaric oxygen Nanjing Zijin Hospital
The principle of non- resistance device for oxygen therapy
used gradient pressure for inhale and exhale oxygen.
Oxygen
pathway:
gas
source
-control
board
-humidification bottles -flow meter - Simulation of the lung
- masks (the trigeminal) - patients - oxygen pipeline outdoors. The patients suitable for non-resistance device
for oxygen therapy are including : 1.The patients can
breath without ventilator.
The patients whose
Respiratory function were relatively poor .2. The high
paraplegia patients can breath spontaneously whose
respiratory function were relatively poor.3. Coma patients
can breath spontaneously.4 . patients whose respiratory
center is damaged can breath spontaneously. 5 the
patients with fractures of the ribs .6 coma patients with
tracheotomy and the patients can move by order .7 the
patients can use luge type ventilator. The advantage is:
easy to operate. Oxygen without resistance. Patient can
inhale pure oxygen. Cabin oxygen concentration is not
raised.
The steps to opreat non-resistance oxygen therapy device:
1. Redeploye the pressure of the gas source to 4.0-6.0 Mp.
2. open the oxygen valve. 3. guide the accompanying staff
to open flowmeter the flow to 400-600 ml, the flow of
children is depended on the tidal volume. 4.
accompanying staff link oxygen masks and bellows
(trigeminal tube) to the patient.
The gas path of non-resistance device for oxygen therapy
is sealed so the gas is pure oxygen. The first class oxygen
concentration is 21% +4 × flow / L.
treatment. Control group (B group n=21): the course of
disease was above ten days, the patients’ condition was
rather stable and their vital sign was normal. The course
of their disease was the stage of rehabilitation. Contrast
the two groups’ effect. Result: The effective rate of A
group is significantly higher than that of B group.(p<0.05)
Conclusion: Earlier and multi-course of hyperbaric
oxygen treatment can shorten awake time, improve
conscious disturbance, elevate healing rate, lower
disability of disease and raise patient’s quality of life.
K127
HBO cured a case of amnesia
PAN Lin
HBO department of Tianjin Hexi Hospital
K129
hyperbaric oxygen treatment on 3 cases of anesthetic
accident coma
Maoxing Yan
The department of hyperbaric oxygen, the hospital
affiliated to neimenggu medical college
300202
The patients is male, 21 years old. memory loss because
of losing his love.He had severe insomnia, did not know
his family members and classmates, who did not know
where he is, did not remember the address, the skull. His
MRI is normal, so he was diagnosed reactive disorder.
Neither western medicine nor traditional Chinese
medicine took effect. So he came to our hospital and tried
hyperbaric oxygen therapy. two days later, his sleep
improved markedly, and his memory recovered.10 days
after the full restoration he returned normal and went back
to school.
Principle of hyperbaric oxygen treatment: high-pressure of
oxygen can increase the blood flow of vertebral
artery ,and increase the relative oxygen partial pressure of
the brainstem reticular activating system. It can activate
neural activity, increase excitatory in EEG and the number
of fast wave , and extent of frequency.in 0.25 Mpa ,HBO
improve the activities of cytochrome oxidase and Na +-K
+-ATP ,at the same time, it keep the balance of Na + /
Ca2 +, reducing the flow of intracellular, reducing
intracellular calcium overload.It normalizes the cellular
membrane’s function .
HBO improves metabolism in sugar, fats, amino acid and
water, keep electrolyte and acid-base balances, increases
the target cells number and functions of insulin receptors.
K128
The hyperbaric oxygen treatment in diffuse axonal
injury
Yuanbi Yang, Zhuan Wang
Hyperbaric oxygen chamber, Department of neurosurgery,
The hospital affiliated to Luzhou medical college
Objective: Observe the clinical effect of hyperbaric
oxygen treatment on diffuse axonal injury, approach the
suitable therapy opportunity and method. Method:
According to patient's condition and course of disease,
Forty-two patients with diffuse axonal injury were
randomly divided into two groups: Earlier hyperbaric
oxygen treatment group(A group n=21): the course of
disease was from three to seven days, the patients
separated themselves from life danger under drug control
or post-operation, then they accepted hyperbaric oxygen
3 patients with anesthetic accident coma accepted
hyperbaric oxygen treatment combined with drug
treatment from the year of 2005 to 2006, they were cured
and discharged.
1.clinical dates: The first patient was twenty-seven year
female, she accepted appendectomy under general
anesthesia in April, 2005, on the way to ward, she was
found respiratory arrest and immediately received
cardiorespiratory resuscitation, ten minutes later, she
recovered spontaneously breathing but had been deep
coma state, twelve hours later, she was transferred to our
hospital and immediately accepted hyperbaric oxygen
treatment, the therapy pressure was 0.25Mpa, oxygen
inhale 30min×2,one time per day, three days later, she
was gradually clear, one weeks later, she was completely
clear, two weeks later, she was cured and discharged, her
brain MRI image was normal. Both the second and third
patients were four year girls, they have been coma
because of congenital heart disease and started to
accepted hyperbaric oxygen treatment on the third day
and the seventh day post-operation respectively, the
therapy pressure was 0.25Mpa, oxygen inhale 30min×2,
one time per day, three weeks and eight weeks
respectively, one person was cured and discharged, the
other was clear but remained intellectual disorder and
growth retardation.
2. Discussion: Anesthetic accident primarily damages
the brain and can make the brain hypoxia and edema.
Hyperbaric oxygen treatment can quickly raise the
pressure of blood oxygen, improve the brain hypoxia and
control the development of the infernal circle of brain
edema. The two patients our discussed accepted
hyperbaric oxygen treatment rather earlier and got a
better prognosis, two-three weeks later, they were cured
and discharged. The other patient accepted hyperbaric
oxygen treatment rather later and got a bad prognosis.
Therefore, when we encounter the anesthetic accident,
we should apply hyperbaric oxygen treatment on the
patients as early as possible, which can reduce the
course of disease and avoid the occurrence of the
residual. Concerning the regimen of hyperbaric oxygen
treatment, we all used atmosphere compression chamber
in our text, the pressure is 0.20-0.25Mpa, the time of
oxygen inhale is 30min×2, which can get satisfactory
effect and avoid side effects.
K130
Effect of Hyperbaric oxygen therapy on 210 cases of
cerebral trauma
Chunfang Li, Yingyu Pan
People's Hospital of Haikou City 570208
Objective: To study the clinical effects of hyperbaric
oxygen treatment on patients of cerebral trauma. Method:
patients of cerebral trauma were divided into four groups,
including: 30 cases of acute brain injury, 5 cases of
persistent vegetative state, 55 cases of convalescence
stage, 120 cases of sequelae stage . Results: after
treatment 38 patients were cured, 110 cases were
markedly effective, 50 cases improved, 12 cases had no
effect, and the total efficiency was 94.3%. Conclusion:In
the patients of cerebral trauma, hyperbaric oxygen
therapy can promote the recovery of consciousness and
reduce the sequelae. In the patients of long-term coma,
persistent vegetative state and serious nerve damage,
Long course of hyperbaric oxygen therapy can
significantly improve the effect and prognosis. Additionly,
It is most difficult to deal with the patients of severe brain
injury and brain hematoma, as it frequently
accompanying vicious increased intracranial pressure, the
prognosis of patients with cerebral hypoxia was also poor,
the early hyperbaric oxygen therapy can increase oxygen
content in time to prevent the occurrence of malignant
hypoxia ischemia, providing the possibility of the recovery
of neurological function. Therefore the patients of cerebral
trauma should be given hyperbaric oxygen therapy as
early as possible, to reduce brain injury mortality and
morbidity, to improve the quality of life of patients. In the
210 cases of cerebral trauma, the patients who had
obvious effects after hyperbaric oxygen treatment mostly
were early onset of illness and not serous condition.
K131
Intelligence Deciding the Therapetic Period
Neonatal Hypoxic-Ischemic Encephalopathy
of
the relationship either between therapeutic period and
effect or between therapeutic period and the rehabilitation
of intelligence. In this regard, combined with the
department of pediatrics, we observed the cases of HIE
since 2001 consecutively. The protocol of HBO therapy is
0.03 Mpa pressure, 20min for 100% oxygen breathing,
40min total therapeutic time with twice washing chamber,
once/d and ten times for a period in a single-person,
pure-oxygen chamber.
K132
Experience of applying the multi-parameter ECG
monitor in hyperbaric oxygen chamber
peiyun Li, mingzh Ma
People's Hospital of Haikou City
With the development of medical science, high-tech
medical equipment has been used more and mor
extensively in hospitals. Having the advantage of
promptness, accuracy and visulization, ECG monitor play
an important role in the diagnosis and treatment of
cardiovascular patients and critically ill patients after
surgery. It is common to use multi-parameter ECG
monitor in some clinical departments, but the application
of this devices in hyperbaric oxygen chamber has been
rarely reported.
From November 2007 to April 2008, we used PM-7000
multi-parameter ECG Monitor to observe 159 cases of
critically ill patients in the hyperbaric oxygen chamber of
YCQ3230-37，we have achieved good results. All the
equipment are of type PM-7000 made by Shenzhen
Mairui company, the monitoring procedure has been
strictly followed and all the equipment components have
been checked to make sure they work well, we observed
and record the electrocardiogram changes. The
application of ECG monitor outside the chamber give
critically ill patients more oppptunity for hyperbaric oxygen
treatment, expanded the scope of HBOT and improve the
safety of patients in the chamber, moreover it is convinent
for the medical staff to observe the patients in the
chamber which is good for the treatment.
W-Q Fan
HaiKou People’s Hospital 570208
Neonatal hypoxic-ischemic encephalopathy (HIE), the
brain damage taking place in the prenatal period, is the
normal disease of pediatrics, featured by the high
incidence. Destitute of immediate therapy, severe
neonatal brain can lead to death, irreversible sequelae
and ensuing heavy affliction to family members, increased
social burden and negative effect to the population quality
in our country. The treatment of HIE by hyperbaric oxygen
(HBO) has been prevailing clinically and achieved better
effect nowadays. However, it is still necessary to probe
K133
Clinical observation of hyperbaric oxygen treatment
on sixty case of neurasthenia
ping yang, guiying he
Kelamayi Central Hospital, Xinjiang, China
Sixty patients have accepted hyperbaric oxygen treatment
in our department since the year of 1991, they got
distinguished effect. We report it as follow:
1 Clinical dates
1.1 General dates: 60 patients between 29 and 66 years
old were included, 20 of them are male, the remains are
female, the mean age of them is 47.5 year. The course of
disease is between 15 days and 21 years.
1.2 clinical manifestations: easy to excited, feeling
weary, sentiment, headache, wakefulness, tension,
agitated, the decrease of memory, as well ad impaired
concentration. EEG shows that the enhanced excitability
of cortex and double side symmetry lower amplitude fast
wave.
1.3 Hyperbaric oxygen treatment: The therapy pressure
was 2.0 ～ 2.4ATA, the patients accepted hyperbaric
oxygen treatment one time per day, oxygen
inhale30/×2+5/ per time, one course of treatment was ten
days.
1.4 The standard of therapeutic effect
1.4.1
Curing:
The
psychiatric
symptom
and
somato-symptom of patients completely disappeared, the
patients feel well and their occupation and viability can
recover to anterior sickness.
1.4.2 Improvement: The psychiatric symptom and
somato-symptom of patients generally disappeared, the
patients can be engaged in mental and physical work.
1.4.3
Invalid:
The
psychiatric
symptom
and
somato-symptom of patients remained unchanged.
1.5 Result: After 4-33 times of hyperbaric oxygen
treatment,14 patients’ neurasthenia were cured,44
patients’ condition were improved, 2 patients’ condition
weren’t improved, total effective rate was 96.7 percent.
2. Discussion
Hyperbaric oxygen treatment can make the brain tissue
obtain sufficient oxygen, improve the metabolic function of
neurocyte and lower the excitability of neurocyte, which
make the physiological activity of cerebral cortex
gradually recover. Hyperbaric oxygen treatment can make
the blood flow of vertebral artery increase, the pressure of
oxygen of brain network and brain stem relatively increase,
which can effectively improve the attenuation condition of
the process of inhibition in cerebral cortex, also can
strengthen the function of its regulating and controlling
subcortex autonomic nervous system, relieve the
symptom of autonomic nervous functional disorder.
Hyperbaric oxygen treatment can lower the heart rate of
the patients who have the symptom of cardiopalmus and
pyknocardia, improve the sleep quality of the patients who
have the sleep disorder, improve the senior activity of
nervous system and raise psychological ability, physical
capacity, intellectual ability, as well as work ability. We
also discover the effect of hyperbaric oxygen treatment on
man is better than woman, the short course of disease is
better than the long course, the patients who accepted
hyperbaric oxygen treatment combined with psychological
treatment can obtain better effect. In order to help the
neurasthenia patients early rehabilitate and decrease the
complaints, in addition to drug treatment, we can extend
and apply hyperbaric oxygen treatment on these patients.
K134
high pressure oxygen treat pig streptococcicosis,one
case report
rongzhen wang xiaohong ding
the First Affiliated Hospital, College of Medicine,Zhejiang
University
Case information: the patient was male, 47 years old,
repeating fever for four days, with unconsciousness and
seizure for two days before admission, accompanying the
symptom of nausea and vomiting, head CT showed
density of cerebral longitudinal fissure was high , bleeding
could not be ruled out , then he was transfered to our
hospital. Physical examination: temperature: 39.4 ℃,
coma, and uneasy, stiff neck, muscular tension of four
limbs was high, Babinski sign of two sides were
suspicious positive,there were ecchymosis in four limbs.
9
Blood white blood cell count showed 19.3 × 10 / l,
neutrophil leukocytes accounted for 93.5 percent,
hemoculture found type 2 Streptococcus suis, confirmed
Streptococcus suis disease, meningoencephalitis type.
He was given dehydration, anti-inflammatory and
rehydration treatment.He was still unclear after 10 days,
then he received hyperbaric oxygen therapy, once every
day, 80 minutes of oxygen inhalation each time, the
middle rest of l0 minutes, the absolute pressure was 2.2
ATA, he was in the dim awareness after five days, he had
reaction to question and was agitated; his consciousness
recovered after treatment of 11 days, brain MRI scan had
no obvious signs of abnormality, hyperbaric oxygen
therapy continued, the patient can walk freely after
treatment of 35 days.
Discussion:
This case was acute and had symptom of fever, chills,
general discomfort, headache, nausea, vomiting,
unconsciousness, seizure, skin ecchymosis, meningeal
irritation sign positive, WBC and neutrophil ratio increased,
CSF showed Septic change, blood culture found type 2
Streptococcus suis, diagnosis of pig-borne disease was
confirmed. on the basis of comprehensive treatment, the
patient was given a daily hyperbaric oxygen treatment,
after HBOT of 11 times, the patient was awake in a stable
condition. HBO, a non-drug treatment, played an
important role in the treatment of this case of human
infected with pig Streptococcus suis. HBO improves the
supply of oxygen to the whole brain or the regional lesions,
help repair damaged cells; hyperbaric oxygen may also
break the vicious cycle of inflammatory - hypoxia cerebral edema - increased intracranial pressure , at the
same time , network activation system has relatively
high oxygen pressure, it accelerates the patient’s
recovery from coma.
K135
Hyperbaric oxygenation and facilitation technique
combination treatment of a brain diffuse atonal injury
Kunru Zhou, Zhihua Yang, Xiuzhen Wang, Jinbo Wang,
Shaoxiong Xu
Guang Dong Shen Zhen Bu Ji Peoples Hospital,
Shenzhen, China
Objective: For the sake of study the therapeutic effect of
Hyperbaric oxygenation and facilitation technique cure a
brain diffuse atonal injury, my hospital carried on the close
observation into 60 cases of brain diffuse atonal injury
patients from 2003 to 2007. Method: 60 cases of brain
diffuse atonal injury patients are randomly divided into 2
sets. Contrast group (24 cases) was pure to given
combined therapy of taking normal regulations medicine:
Dehydrating agent depress encephalic pressure;
adrenocortical hormones activated reticular formation for
awakened cerebrum; correct electrolyte maladjustment;
medicine nourish brain cell supply energy; acupuncture
and manipulation; HBO group (36 cases) was given
combined therapy of taking normal regulations medicine
and hyperbaric oxygenation and facilitation technique,
started on the sixth day after the patient hospitalized.
Result: The treatment set cures 25, show effect 7, valid 3,
invalid 1, total efficient 97.5%. The Contrast set cures 8,
show effect 4, valid 5, invalid 7, total efficient 70.8%.
Treatment set efficient is obviously better than the
contrast set ;( p<0.05) Reply to diagnose after a year or
the telephone track, the treatment set linger effect
incidence rate is 11.1%. The Contrast set linger effect
incidence rate is 58.4%, 2 sets relatively have obvious
difference.
(p<0.01).
Conclusion:
Hyperbaric
oxygenation and facilitation technique cure a brain diffuse
atonal injury earlier, recover quicker, cure effect better.
Namely educed disability rate, also reduced death rate.
Promote the patient of the function recover, make the
patient be able to return to a society as early as possible.
K136
The Effect of Hyperbaric Oxygen Therapy on cerebral
infarction
Pan xiaorong, Fan qiuping
People's Hospital of Guangxi Zhuang Autonomous
Region
Our department use hyperbaric oxygen therapy to treat
120 patients with cerebral infarction supplemented with
drug treatment since March 1996 to March 2006, with
the same period 120 cases of conventional drug therapy
in patients with cerebral infarction contrast, have made
significant effect, the report are as follows:
1 information and methods
1.1 general information:
240 cases of cerebral infarction patients, 154 mailes, 86
females, ages 45 to 77 , an average of 63.5 years old, the
duration of suffering is from one day to two and a half
years, of which 181 cases of basal ganglia infarction, 22
cases of multiple infarction (Infarction lesions in the three
above), lacunar infarction in 37 cases. Randomly divided
into two groups: ⑴ HBO therapy and conventional drug
treatment group ,120 cases (integrated group); ⑵
conventional drug therapy group of 120 cases (the control
group). According to "stroke in patients with clinical
neurological impairment score standards" (1995) [2] score.
The two groups by age, sex and treatment of nerve
impairment and there was no significant difference.
1.2 treatment:
two groups of patients using conventional dehydration
drugs decreasing intracranial pressure, blood vessel
activation of nerve agents and expansion of vitamin E,
and so on. Hyperbaric oxygen treatment: treatment of
pressure 0.2 Mpa, wearing oxygen masks inhale pure
oxygen of 30 minutes, rest for five minutes to inhale the
cabin air, and then inhale pure oxygen for 30
minutes.Patients got HBOT Once a day of every 10 times
for a course of treatment. A total of 2 to 10 courses,
follow-up 1 year, then statistic further recurrence rate of
cerebral infarction.
Results
Two patients with cerebral infarction treatment
comparison in table 1. The course of integrated group and
effects of table 2.
Table 1 Comparison therapeutic effect of the two groups
cured
markedlytotal
Numb effective effective failed
effecti
er
ve rate
number
of
of
（%）
cases
cases %
cases%
cases %
c
ases %
integrated group
120 96 80.0
12 10.0
9 7.5
3 2.
5
97.5
Control
group 120 48 40.0
36 30.0
21 7.5
15 12.5
87.5
Compared with the control group x2=10.4, P＜0.01
Table 2 :The course of integrated group with the effects of
disease
course Number cured Markedly effective failed total
of
effective
effective
cases
rate（%）
21
15
4
0
100
1
～40
28
7
13
8
0
100
30days
32
18
13
1
96.87
31
～20
4
14
2
90
60days
61
～
90days
91days ～
2.5years
Differences between the two groups have a very
significant (P <0.01), shows that integrated group relapse
rate lower than the control group.
3 discussion
hyperbaric oxygen,① blood oxygen content increase，
blood oxygen pressure increase. Oxygen diffusing
capacity and the diffusion distance increase, reducing
brain cell degeneration and necrosis due to asphyxia, the
effective restoration of ischemia " penumbral regions"
cell function. ② enhanced aerobic metabolism, reduce
anaerobic glycolysis, reducing brain lactate concentration,
and thereby redress acid poisoning, improved the brain
environment. ③ contraction of blood vessels, reducing
cerebral edema and reduce intracranial pressure. ④
promote the formation of collateral circulation. ⑤
increase vertebral - basal blood flow and is conducive to
the unconscious patients restoration of consciousness.
⑥ lower blood viscosity, promote thrombolisis and
absorption. ⑦ reduce reperfusion injury to the brain.
Not only hyperbaric oxygen therapy can be used in
cerebral infarction but also can prevent the cerebral
infarction recurrence again, it is an effective, safe and
reliable rehabilitation measures, have important clinical
significance, it is worth promoting. In view of oxygen
under high pressure oxygen from the dispersion is still
limited, the choice of cases with small lesions will have
better effect.
K137
Effects of hyperbaric oxygen on sports ability in
female middle- long distance athletes before matches
1
2
Jian Zhang , Lunying Sun
1. HBO Department of Shandong Qianfoshan Hospital,
Shandong, China
2. PE College of Shandong economic college, Shandong,
China
Objective: To explore the effects of hyperbaric oxygen
(HBO) on sports ability in female middle- long distance
athletes before matches. Methods: Twenty-three female
1500 meter athletes were randomly divided into
hyperbaric oxygen group (HBO group, 11 cases) and
normal pressure air group (NPA group, 12 cases). Before
matches, eleven athletes (HBO group) were exposed to a
hyperbaric environment of 0.2MPa (2.0ATA) to accept a
treatment of hyperbaric oxygen. The time of rising
pressure was 20 min.the athletes inhale pure oxygen for
60 min, taking a rest for 10 min to breathe air among
inhaling oxygen. The time of reducing pressure was 30
min. The athletes in NPA group were exposed to a
ordinary pressure cabin for 120 min, breathing air. Their
quiet heart rate (HR), fatigue level, and the results of 1500
meter were determined respectively before and after
experiment. Results: Before experiment, all indexes of
two groups were not significantly different .After
experiment, the HR of athletes in HBO group were
average lower 3.08±0.83 beats each minute than before
experiment ,and also the feeling of fatigue and the results
of 1500m(female) were obviously improved as compaired
with before experiment. All indexes in HBO group were
much more improvement than those in NPA group.
Conclution: Before matches, HBO treatment can
increase the content of oxygen in blood and tissues ,
reducing the heart rate, lightening fatigue, adjusting the
function of brain cortex ,and boosting female middle- long
distance athletes the motor excitability .
K138
the Influence of HBO to the Serous Endothelin in
Patients of Serebral Infarction
H-K Pan
the First Hospital affiliated to ZhongShan University
510060
Objective: The study was designed to probe the influence
of hperbaric oxygen (HBO) to the serous endothelin in
patients of cerebral infarction. Methods: Assessing the
relationship between the concentration of serous
endothelin and therapeutic effect to 64 patients of cerebral
infarction treated by HBO. Results: the patients within 2w
sick have the significantly increased serous endothelin
compared with pretreatment (P<0.05), while patients with
beyond 2w sick have significantly abating serous
endothelin （ P<0.05 ） . In comparison to the a bit of
enhancement of control groups, the patients treated by
HBO showed great climbing serous endothelin, especially
those within 2w sick (P<0.05). Conclusion: According to
our study, the beneficial effect of HBO to cerebral
infarction has nothing to do with the decreased serous
endothelin and possible related approaches need to be
further explored.
K139
A Case Report: the treatment of Hyperbaric Oxygen to
osteomyelitis
haibin xiang, rong xie
Shanghai Punan hospital 200125
A female, 8 years old patient went to some hospital in
ShangHai for the suddenly occurred weakness of low
limbs and ensuing walking hardly accompanied with
disabled urinary and fecal discharge. Physical
examination: Reflex of patellar tendon of both sides does
not exist. The muscular tension is low and muscle force of
the low limbs is level O－Ⅰwith negative Babinski Sign.
The patient was diagnosed as osteomyelitis preliminarily
and hospitalized. Inpatient examination: oligoclonal
antibody of CSF indicated the damaged blood-brain
barrier and disproportionated increased ratio of IgG.
Spine MRI showed the abnormal signals(T4 － T11).
Treatment: 200mg methylprednisolone ivgtt for the first
day, 250mg/d for the second and third day and following
prednisone 40mg/d P0 adding the use of γ-Globulin and
ATP. On the third day, she restored urinary discharge and
on the ninth day, she had the ability of fecal discharge
with still existing weak low limbs. HBO therapy began with
on the tenth day of hospitalization. After the first period of
HBO therapy, patient showed restored urinary and fecal
discharge completely, enhanced muscle force to level Ⅲ
－Ⅳ, increased muscle tension, rehabilitated reflex of
patellar tendon, positive pathological reflex and she could
walk supported by others. The progress after the second
therapeutic period involved walking by herself, the
asymmetrically induced reflex of patellar tendon and
promoted muscle force to level Ⅳ—Ⅴ.
For the ineffective normal treatment, we apply her HBO
treatment timely and the significantly increased muscle
force in low limbs suggested the nice synergic function of
HBO and normal treatment. The possible effect that HBO
treatment have in the case as following: increasing the
oxygen pressure and concentration of bone marrow and
enhancing the oxygen extension, which accelerating the
rectification of local hypoxia condition; reducing the spinal
cord edema; ameliorating the function of blood-spinal cord
barrier; abating the tissue adherence after local infection;
prolonging the reproduction period of damaged neural
cells and protecting the irreversibly damaged neural
tissue which contribute to the rehabilitation of neural
function; decreasing the occurrence of delayed paralysis
given rise to by the dysfunctional blood flow of the spinal
cord.
K140
The discussion of usage of electronic medical
equipments in hyperbaric oxygen chambers
Zhu JianMing
Xin Hua Hospital Affiliated to Shanghai Jiao Tong
University School of Medicine, Shanghai, China
When hyperbaric oxygen chamber works, it is a confined
manned pressure vessel, so it is a very important issue
that the proper usage of electronic medical equipments in
the cabin, which is related to the guarantee of the safety
and effectiveness of the treatment. When the various
equipment in the cabin are in an oxygen-rich environment
and play the redox reactions, so the board can not have a
monitor within the EDM, voltage can not exceed 24 V,
which is order to prevent high-pressure environment from
the impact and interference, and accurately monitor
patients’ various and real-time physiological information
who are inside the cabin for a long time. Health-care
workers can observe them convenient inside or outside
the cabin, and set and adjust parameters of change at any
time. Multi-parameter monitor is composed of two parts:
multiple physiological parameters box which is general at
the oxygen chamber and is used to measure the patient's
ECG, SpO2, NIBP, TEMP, and the physiological
parameter of the end of breathing carbon dioxide.
Through the standard RS232 socket and data
transmission lines, the patient's information can be easily
delivered to the computer workstations outside the
hyperbaric oxygen chamber whose general operating
voltage is DC 12 V and can be safely used in the
oxygen-rich environment. The purpose of usage of
computer workstations is to read the patient information
for analysis, storage, processing and link to HIS systems
in hospitals to share resources. LCD displays are
generally used in the oxygen chamber, and LCD is
generally divided into two main materials which are called
LCD and TFT respectively. The LCD screen usually
needs LED lights background, and also need high-voltage
lights which can light background lights. So the LCD is
liquid crystal display of the power supply DC 12 V, but in
order to supply background lights around 300 ~ 100 V
high-pressure, it needs a inverter power supply. It is
precisely this power of the high-voltage inverter, which
brings about the hidden dangers to patients who use the
LCD screen of multi-parameter monitors in the hyperbaric
oxygen chamber. When the oxygen concentration in the
high-pressure oxygen chamber is beyond the standard,
the high pressure about 300 ~ 100 V will produce "tinder"
phenomenon, and it is easy to bring about burning
accidents. The TFT (Thin Film Transistor), which is called
the film field-effect transistor, has made crystal electrodes
through using thin-film technology, and control the
opening and closing of any one point through using the
method of scanning. It needn’t use LED lights back to light
the liquid crystal display, so it has no high-pressure, and is
securer to use in the high-pressure oxygen chamber.
Discussion: We must pay attention to the materials of
liquid crystal display of the monitor which is used in the
cabin and use TFT LCD screen to the greatest possible
extent. Medical electronics, pneumatic equipment which
are used in the oxygen chamber could bring about
problems of high-voltage, superficial views on the author,
related departments should plan as soon as possible, to
make detailed laws and regulations of the usage of
electronic medical equipment which is in the hyperbaric
oxygen chamber, and to ensure the safety of usage.
K141
Preventive
maintenance
of
equipments
and
hyperbaric oxygen chamber
Zhu JianMing, Yuan YunLai
Xin Hua Hospital Affiliated to Shanghai Jiao Tong
University School of Medicine, Shanghai, China
When hyperbaric oxygen chambers are at work, they are
confined manned pressure containers, so the safety is a
very important issue, the paper will be on the way to do a
study about Preventive Maintenance. Background: It is a
very important issue to ensure the safety of the usage of
oxygen chambers. In the daily usage of the oxygen
chamber, routine maintenance work must be done well
rather than repair it when it is out of work, which is to
ensure the safety and effectiveness of treatments. The
requirements of preventive maintenance: 1. When oxygen
chambers are at work, they are in the oxygen-rich
environment, so do remember to pay attention to the
range of lubricants when using PM. 2. Generally speaking,
a small amount of lubricating oil can be used only when
the internal of oxygen chambers in addition to the
revolving door to add flexibility, and other parts for
lubricating oil is prohibited. 3. PM asked to reflect "P", is
Preventive, so the cycle of doing PM should be classified
according to the frequency of usage of hyperbaric oxygen
chambers, at least twice a year. The implementation of
preventive maintenance: 1. As far as possible to set a
relatively fixed date, do not arbitrarily extend the cycle of
PM. 2. In addition to preparing the appropriate cleaning
supplies, we must also prepare a strong suction vacuum
cleaner and other equipments. 3. We must prepare
silicone oil according to the characteristics of hyperbaric
oxygen chambers. 4. To the devices, instrumentations
and equipments inside the oxygen chambers, case work
such as cleaning dust should be done. 5. First of all, a test
module should be done on equipments of oxygen
chambers to observe and monitor the real-time working
conditions for the record, and after the end of PM, we
should control the situation before and after. 6. Safety
teats, current tests and inspection of the grounding
impedance should be done to equipments inside
chambers. 7. After the end of PM, all work should be on
record and classified archive for verification in the future.
The discussion of preventive maintenance: It can extend
the life of hyperbaric oxygen chamber through PM, and
now the instruments are used within the SMT
surface-mount technology to assemble electronic
components, IC-pin is only 1 mm spacing, so if there is
dust and plot the event of a humid air, pin indirectly on a
larger on-resistance phenomenon have caused the
breakdown between the pin-damaged. It will be more
good rather than harm to do PM regularly, of course,
when doing PM, carefully formulated work must be done,
and the equipment within the CMOS circuitry must be
careful noticed and not use vacuum cleaner to suction
circuit, which will cause the possibility that circuit vacuum
cleaner was hit head on the electrostatic Wearing. We
should take Preventive Maintenance as a main way, and
take repairing as a supplemented way to extend the
service life of hyperbaric oxygen chambers and save
valuable resources of medical equipments and increase
the profits of hospitals.
K142
Analysis of 12 cases of the common fault in
hyperbaric oxygen chamber
Zhu JianMing, Yuan YunLai
Xin Hua Hospital Affiliated to Shanghai Jiao Tong
University School of Medicine, Shanghai, China
When the hyperbaric oxygen chamber works, it is an
airtight pressure vessel carries people. The most
important problem is to make use of security. Based on
my years of maintenance repair experience in medical
equipment including hyperbaric oxygen chamber, I will
explore the common fault in hyperbaric oxygen chamber
below. The integrity of hyperbaric oxygen chamber is the
primary guarantee to ensure the use of safety and
treatment and to keep the oxygen chamber in the normal
work state is the prerequisite of hyperbaric oxygen
therapy. Below makes a discussion to the common fault in
the use of hyperbaric oxygen equipment. Common fault in
the use of hyperbaric oxygen chamber subsidiary
equipment: 1. At the use of the walkie-talkie of the oxygen
chamber occasionally leads to “ broadcast ” voice of the
radio broadcast interference in the middle of the night,
would seriously affect the signal communication between
in and outside the chamber. The main reason is 220V the
city electricity 50Hz power frequency disturbance string
amplifier, returned to original state “the broadcast sound”.
Solution: (1) Improve the grounding of the walkie-talkies
to make the grounding more reliable. (2) Place a capacitor
capacity from 0.01 µ f to 0.01 µ f on the secondarily power
transformer of the walkie-talkie in order to reduce the
disturbance phenomenon by the distributed capacitor.
(3) Use the transformers with center shielding layer
between first and secondary to replace the original one.
(4) Change the moving coil microphone and speaker
which used in chamber into the piezoelectric ceramic chip.
2. The lung type oxygen used in oxygen chamber is
specially designed for the driver attracts the oxygen
equipment, needs about 300Pa negative pressure to turn
on the rocking bar valve to attract the oxygen, when no
oxygen absorption in failure, refer to the following steps to
inspect: (1) Inspects whether the rubber cone-way valve
piece in the three-way pipe has the aging and anti-roll or
shifting bumps into attracts the oxygen tube collar
extension. (Row of oxygen breakdown reverse inspection
row of oxygen tube.) If found the rubber one -way valve
has the aged phenomenon, exchange a new one. If the
one-way valve bumps into three contacts collar extension,
install once again, not bump into the collar extension as
suitably. (2) Check the interior feeling press diaphragm
of the lung type oxygen whether it would produce the
aged chap in the course of time, if aging exchange a new
diaphragm. (3) The rocking bar valve module like occurs
loses the elasticity, cannot work when the normal work
scope, must adjust the module operating region or use in
exchange the new module. (4) The inspection lung type
oxygen supply with for the oxygen pipeline attachment
point, discovered when has the air leakage phenomenon,
must disassemble the attachment point, changes into
newly the aged O circle. 3. The use of self-flow oxygen in
oxygen chamber: In the oxygen cabin uses flows
automatically the oxygen general procedure uses the lung
type oxygen supply, adjusts the screw rod to move most
ambassador the oxygen to supply continuously, supplies
the oxygen with the face guard to the patient. This method
author thought improper, not only adjusts the biggest time
oxygen from to supply the oxygen pipe flow to leave also
can “see the seam from the lung type oxygen supply to
drill” in all directions the overflow, creates in the cabin the
oxygen concentration to elevate suddenly. In lung type
oxygen supply for an oxygen pipeline connection place
addition three contacts connection, moreover meets one
for the oxygen tube, connects in parallel fashion into a
small oxygen flow idea, follows the current on the gauge
outlet the direct supplies to have to use flows
automatically the oxygen patient, instead reduced in the
oxygen cabin oxygen concentration. 4. the problem in
the use of the rescue of medical equipment in hyperbaric
oxygen chamber. The 220V alternating current supply
cannot enter the oxygen chamber is advantaged in the
personal safety, but also limited the rescue medical
equipment to enter the oxygen chamber. There are many
air operated medical equipment (with rechargeable
battery inside) to be possible to supply in the oxygen
chamber to use. That large-scale oxygen chamber needs
frequently to rescue the seriously injured patient in the
oxygen cabin must install the special-purpose fast oxygen
connection in the chamber for the oxygen pipeline on. 5.
The common problem on the equipment supplies the
oxygen in the hyperbaric oxygen chamber. Enters the
cabin after the oxygen pipeline to divide into the left and
right two group supplies lung type oxygen supply, such
procedure can produce inevitably for the oxygen pipeline
“the blind end”, causes “the blind end” on patient to attract
the oxygen to feel the hard sledding. Installs one in each
lung type oxygen supply for the oxygen pipeline
attachment point “the cushion pot” to solve, “the cushion
pot” must have the volume which the enough big like 2
litres control. 6. The problem in the installment of the lung
type oxygen supply in oxygen chamber. In the oxygen
cabin the lung type oxygen supply installment direction
common is all kinds, there are the top and bottom
installment and the left and right one. The correct
installment direction of lung type oxygen supply should be
according to supplies under the oxygen tube vertical
dynasty, adjusts the screw rod to be vertical in on installs.
This way is the most reasonable one, the patient attracts
when the oxygen not only depends on the negative
pressure the function, moreover has the action of gravity
help to make an effort slightly. 7. The daily adjustment
method of the lung type oxygen supply. From attracts on
the oxygen tube the plastic three-way pipe to draw out
places nearby the ear on, adjusts the screw rod
dextrorotation, when just heard “si si” leaked the oxygen
sound, the screw rod maneuver half-turn, the lung type
oxygen supply is adjusted by now in “the critical point”,
attracted the oxygen to be at the optimum condition to
make an effort slightly. 8. The problem in the supply of
oxygen source in the oxygen bottle. The problem
produced by the valve gasket which between the bus and
the oxygen cylinder is that in the use of oxygen bottles
(pressure is 15MPa/150KPa) as a source of oxygen
supply, because of the use of nylon or Teflon used as the
gasket material for valve interior, some articles reported
frequently that a valve gasket in the combustion. The
ignition point temperature of Teflon is 300℃, not easy to
burn, so it is safety to use the gasket which is made by
material of the pure Teflon, but uses the gasket which the
material does on is easily to be a burning accident.
Completely change the Teflon gasket in the oxygen
supply pipeline into the red copper material so that once
and for all solves the burning hidden danger which
possibly exists. 9. Console oxygen supply oxygen
chamber pressure gauges, indicators can not return to the
issue. Installs an evacuation valve between the secondary
reducer and the control bench for the oxygen valve can
solve the problem. After the daily payload separation
ended, close the oxygen valve then open the evacuate
valve, drain away completely the pipeline in remaining
oxygen causes the indicator to return the zero. It can
extend the life of the oxygen pressure gauge that close
the oxygen valve and then immediately evacuate the
remaining oxygen in the oxygen pipeline, protect the
oxygen pressure gauge when the secondary reducer
sudden malfunction and the oxygen gauge hand ultra time
limit. 10. The problem in the compressed air used as the
compression medium. The air pressure cabin uses the
compressed air to take the compression medium when
treat patient, its quality is related with patient's safety and
the effect of the treatment directly. In medical oxygen
cabin GB code stipulated the compressed air must store
up period of time after the preparation to be able to use,
but in fact can achieve the oxygen cabin is few. In my
humble opinion, the compressed air used in the oxygen
cabin should refer to the standard of the military symbol
"Diving Breath Gas" to carry out to maintain patient's
treatment benefit. The first choice of vents one's anger
with air pressure is non-oil air compressor. Install the heat
interchanger behind the air outlet of the compressor,
which cause the compressed air temperature prepared
dropped greatly and reduce the presence of hydrocarbon
in the compressed air. Install the multistage fluids behind
the export mouth in the heat interchanger separator to
reduce the hydrocarbon again. Conditional liquid oxygen
chamber should increase behind the gas separator with a
dedicated dry precision porous filter so that the
preparation of the compressed air would be a better
quality. 11. The selection of the activated carbon in
oxygen chamber. The round circle columnar husk should
be the first choice of the activated carbon in oxygen
chamber supply air filters, because the graphite activated
carbon is refined from the coal and the coal-tar oil, but
0.3MPa the air feed limiting pressure can run out the
micro granulated activated carbon from the air strainer,
therefore the graphite, the micro granulated activated
carbon does not suit in the oxygen cabin air feed air
strainer to use.
K143
Curative effect of the treatment with HBO for
ulcerative colitis
Zhu Hourong
NanJing JiangNing Hospital, Nanjing, China
Objective: To evaluate the curative effect of the
combined treatment with Sulfasalazine and Hyperbaric
oxygenation (HBO) for ulcerative colitis. Methods: 38
cases with light or moderate ulcerative colitis at active
stage were randomly divided into 2 groups, treatment
group(n=19) receiving sulfasalazine and HBO, and control
group(n=19), receiving sulfasalazine only, the curative
effect, the reoccurrence in 6 months,the change of main
symptoms before and after treatment were evaluated。
Results: The total effective rate of treatment group
(89.47%) was significantly higher than that of control
group(68.42%) (P<0.05), there existed an obvious
difference in symptom relief, such as abdominal pain,
diarrhea, mucus bloody stool and tenesmus, between the
2 group after treatment (P<0.05). Conclusions: the
combined treatment with Sulfasalazine and HBO is of
better curative effect for ulcerative colitis and it can reduce
the reoccurrence.
K144
The research on treating hypoxic ischemic
encephalopathy by hyperbaric oxygen treatment and
the changes of cytokines
Zhou Yihong, Chan Guiyang, Tang Lanfen, Ao Dang
Department of Pediatrics, Affiliated Hospital of Guang
Dong Medical College, Zhanjiang, China
Objective: To investigate the changes of serum
Interleukin-6(IL-6), Tumor necrosis factor-α (TNF-α) and
Insulin-like growth factor-1 (IGF-1) in hypoxic ischemic
encephalopathy(HIE) by hyperbaric oxygen treatment
(HBOT) and clinical significance. Methods: Fourty-eight
neonates with HIE were divided into two groups. Routine
treatment group were intervened with the conventional
treatment, hyperbaric oxygenation group in addition to the
conventional treatment. Serum IL-6, TNF-α and IGF-1 in
48 cases of HIE were detected by radio-immunity. Result:
The increaseed range of serum IL-6 in HBOT group were
significantly higher than routine therapy group (P < 0. 05);
the decreased range of serum TNF-α and IGF-1 in HBOT
group were significantly higher than routine therapy group
(P < 0. 05). Conclusion:
HBOT could increase the
production of IL-6 and reduce the production of TNF-α
and IGF-1 . HBOT is an effective therapy for hypoxic and
ischemic brain damage.
K145
Hyperbaric oxygen therapy adhesive effect of 114
cases of obstruction
Yubao Zhang
Lei Ge
Shumin Meng
Wen Zhai
Fuqin Wang
The Frist Peoples Hospital of Harbin, Ha’erbin, China
Objective: To investigate the high-pressure oxygen (HBO)
for the effect of adhesive intestinal obstruction. Methods:
114 cases of postoperative adhesions with obstruction
were randomly divided into two groups, the control group
using conventional drug treatment. HBO group, in addition
to conventional drugs, the daily 0.25 MPa pressure,
oxygen absorption 60 minutes (10 minutes to break the
middle), a total of 20 days. Results: 20 days later, HBO
group cure rate significantly higher than that, there was a
significant difference, statistical significance. Conclusion:
The high-pressure oxygen therapy can increase the
intestinal wall of the blood supply adequate oxygen supply,
accelerate intestinal peristalsis, and promote the
absorption of inflammatory mediators, in the treatment of
adhesive obstruction plays a role should not be
underestimated, is an effective treatment measures.
K146
Hyperbaric oxygen chamber, hospital infection
control
Haiying Qiu, Yan Zhao, Ji Wang, Lihua Liu, Rui Li
The Frist Peoples Hospital of Harbin, Ha’erbin, China
Objective: To eliminate nosocomial infections, control of
high-pressure oxygen chamber and ancillary devices
colonies of bacteria to ensure that the hyperbaric oxygen
therapy effective. Method: Siebenpfeiffer disinfectants
and ultraviolet light to the oxygen chamber of the air,
oxygen subsidiary devices were disinfected. Results: air
control colonies in ≤500cfu/M3, articles colonies in the
control of ≤10cfu/M3, put an end to the oxygen chamber
treatment in hospital infection. Conclusion: The weekly
regular disinfection of the patient and reasonable
arrangements for a special event, special disinfection, to
put an end to high-pressure oxygen treatment of hospital
infection.
K147
HBO treat meniere syndrome 66 cases
1
2
2
2
Danhong Song , Wen Zhai , Pei Liu , Xiaohua Li ,
2
Hong Li
1. The second Peoples Hospital of Harbin, Ha’erbin,
China
2. The Frist Peoples Hospital of Harbin, Ha’erbin, China
Purpose: Detection HBO treats Meniere Syndrome (MS)
clinic curative effect. Method: There are 2 groups, 66
cases. Except HBO group routine medication of dilatated
blood vessels, there are HBO pressure 0.20 mpa and
absorb pure oxygen 60 mins (break 10 mins), once per
day, total 15 times. Comparison group adopt routine
medication of dilatated blood vessels. Result: HBO treat
MS total efficient rate 95.8%.Comparing with comparison
group, diversity is obviously (P<0.05). Enforcing position
in 7 days, fear reduced earlier (P<0.05). Conclusion:
HBO treat MS is one of curative effect at present.
K148
Observation on the curative effect of HBO on treating
continuing vegetative state
Xuming Zhou, Haiying Qiu, Qun Cai, Huiting Cong,
Ji Wang
The Frist Hospital of Ha’erbin, China
Purpose: Detection HBO curative effect observation of
treat continuing vegetative state (CVS). Method: Random
sample divided 2 groups of HBO treatment and
comparison. Except HBO group routine medication
treatment, there are HBO pressure 0.25mpa and absorb
pure oxygen 60 mins (break 10 mins), once per day, total
20-70 times. Comparison group routine medication
treatment. Result: Curative effect of HBO group
surpassed comparison group (P<0.01), which is early and
long period of treatment are better curative effect.
Conclusion: HBO which is importance of CVS routine
treatment.
K149
Treating 180 cases of paroxysmal deafness by using
HBO combined with acusector
Xuming Zhou, Shumin Meng, Lei Ge, Bo Zhai, Bo Dong
The Frist Hospital of Ha’erbin, China
Purpose: Discussion HBO result of HBO combines
abusector which treats paroxysmal deafness (PD)
Method: Random sample there are 2 groups which 180
PD HBO and comparison. Group HBO: Except routine
treat with acusector and medication. Meanwhile, there are
HBO pressure 0.25mpa and absorb pure oxygen 60 mins
(break 10 mins), once per day, total 10-30 times. Group
comparison
routine
treat
with
acusector
and
medication.Meanwhile, sickness in a week and over a
week treat with HBO and comparison with PD under
treatment of routine acusector and medication. Result:
Group HBO for PD curative effect rate and getting better
rate and more than over a week obviously (P<0.01).
Group in a week curative effect rate and getting better rate
are more than over a week HBO treatment group(P<0.05).
Diversity exist prominence and significance of statistics.
Conclusion: HBO combine acusector and medication
which treat PD are better than simple acusector and
medication obviously. Meanwhile earlier treatment is
better for curative effect.
K150
Hyperbaric oxygen treating 32 cases of metal
poisoning
Li Wen, Zhang ZhiGe, Tuo LiuYing, Yang Meiping, Shi
Zhongheng
Department of Hyperbaric Oxygen, Jiangbin Hospital of
Guangxi
We applicated hyperbaric oxygen (HBO) treatment in 32
cases of metal poisoning in November 1996 to December
2007, and compared to similar cases who received
conventional treatment in November 1987 to November
1996, there was significant difference, it is report as
follows: Clinical data: 1. General information: There were
63 cases, 56 cases from the production workers, 9 cases
of mistaken consumption poisoning. 32 cases of HBO
treatment group, 20 cases men, 12 women, aged 22-55
years old, of which 9 cases of lead poisoning, 12 cases of
manganese poisoning, 9 cases of tin poisoning, 2 cases
of arsenic poisoning. 31 cases of control group 19 men,
12 women, aged 20-56 years old, of which 9 cases of lead
poisoning, 11 cases of manganese poisoning, tin
poisoning in 10 cases, 1 case of arsenic poisoning. Two
groups had almost the same performance. 2. Clinical
performance:
there
are
different
levels
of
unconsciousness in 12 cases and the rest had the
performance of headache, dizziness, fatigue, insomnia,
nausea, vomiting, tremor, polyuria and so on. 3.
Treatment: two groups used metal driving, symptomatic
treatment and support treatment. HBO treatment group
used medium-sized hyperbaric oxygen chamber,
compressing air to 0.2 MPa, inhaling oxygen for 80
minutes, the middle rest for 5-10 minutes,once a day, 12
times was 1 course, general 2-3 courses, up to 5 courses.
4. Efficacy evaluation standard: full recovery:
consciousness and other symptoms are back to normal.
markedly effective: consciousness has
returned to
normal, but the signs have not been completely back to
normal. improveing: symptoms and signs have got better
slightly after treatment. invalid: signs and symptoms did
not change after treatment. Results: effect of HBO
treatment group were: 11 cases full recovery, 11 cases
markedly effective, 7 cases improved, 3 cases invalid, and
the total efficiency was 90.6 percent. Control group: 5
cases full recovery, 8 cases markedly effective, 9 cases
improved, 9 cases invalid, the total efficiency was 70.9
percent. There was significant difference in two groups, P
<0.05. Discuss: the mechanism of HBO treatment in
metal poisoning is to rapidly improve the state of hypoxia,
to interrupt the vicious cycle of edema, so it can effectively
alleviate symptoms. HBO can increase blood oxygen
pressure, improve the metabolism of brain cells, reverse
or delay the degeneration of brain cells caused by the
poisoning, maintain the flexibility and toughness of red
blood cells and reduce the occurrence of hemolysis. This
observation of HBO treatment in metal poisoning had
quite satisfactory results. So for the patients of metal
poisoning,we suggest take early HBO treatment on the
basis of metal driving and symptomatic treatment,
patients of chronic poisoning should increase more
courses appropriately.
K151
Treating a case of scald by HBO
Haiping Yang, Juan Chang, Chuanying Sun
Youanmen Hospital of Beijing hospitail association,
Beijing, China
This article reported a case of scald by boil water, who is a
diabetic woman, was treated by HBO. The wounds of her
legs caused a long delay, had infected and fever. After 26
times HBOT, the wounds were recovered.The treatment
principls of scald by HBO were discussed in this report.
K152
The clinical application of a new oxygen
inhalation-exsufflation
device
with
emergency
intra-chamber atomization and humidification
Youcun Wang, Peizhen Shi, Jianwei Xu, Zengshan Liu
The department of hyperbaric oxygen, General Hospital of
Jinan military area, Jinan, China
The manufacture and clinical application of a new oxygen
inhalation-exsufflation
device
with
emergency
intra-chamber atomization and humidification were
reported. Without any defect that the traditional device
has, the new device was work-reliable and
simple-operated. The application of the new device in
hyperbaric oxygen chamber increased the methods of
intra-chamber emergency treatment, the indications and
the rescue capability of the chamber, as well as improved
the hygienic criterion of intra-chamber oxygen-supply and
the effects of treatments. It could also eliminate the
hidden danger of accidents.
K153
A case of using hyperbaric oxygen to treat mathenol
poisoning
Yalin Wang, Xiujie Li, Rong Li
Dalian Center Hospital, Dalian, China
A patient, male, 40 years old, suffered from sudden
serious stomach ache for 2 hours during working,
accompanied by nausea and vomiting. He touched
methanol in working environment. Preliminary diagnosis:
the stomach ache requires further examination. Two
hours later, the illness severity increased. His awareness
became unclear, accompanied by breathing difficulty, limb
getting cold, cyanosis, systemic tinea versicolor, no urine,
and BP 90/60 mmHg. Examination in laboratory shows: K
5.63mmol/L, BUN 12mmol/L, Cr 315umol/L, artery blood
Pa CO213mmHg, PH6.8. Diagnosis: methanol poisoning,
acute renal failure, metabolic acidosis, hyperkalemia, and
distributed shock. The treatments to the patient include
orotracheal intubation and mechanical ventilation,
puncturing administration to right internal jugular vein, and
hemofiltration. 24 hours later, the patient’s awareness
recovered, the amount of urine returned to the normal
level, and he could make conversations. We stopped
mechanical ventilation. The patient said that he could not
see objects. Ophthalmology consultation: his eyes had no
light perception; the pupils dilated, D=5mm; direct and
indirect light reflex were both weak. We use energy
mixture, neurotrophic drugs to improve microcirculation
and use Hyperbaric Oxygen (HBO) therapy. 48 hours
after the patient entered the hospital, we performed HBO
therapy. Treatment Program: surface pressure 0.12Mpa,
oxygen inspiring time 60min, once per day. 32 days after
the patient received HBO treatment, his eye sight
recovered completely. Discussion: The patient is a
typical patient suffering from methanol poisoning, the
diagnosis is clear, and the HBO therapy is effective. The
purposes of using hyperbaric oxygen therapy in the
treatment include: 1. HBO helps to improve the activity of
cytochrome oxidase, to inhibit the generation of formic
acid and lactic acid, and to ease the acidosis. 2. HBO has
positive effect to repair and regenerate damaged nerves,
and helps to recover the function of optical nerves. 3.
HBO accelerates the regeneration of damaged capillaries,
provides oxygen for the establishment of circulation in
branch retina, improves the hypoxia status of the fundus
tissue, and prevents pathological changes of inner cells of
retina caused by ischemic and hypoxia. 4. The formic acid
generated during the methanol metabolism is also toxic to
the retina. Hyperbaric oxygen may enhance the activity of
formyl-H4 folate dehydrogenase, further promote the
metabolism of formal acid. On the whole, the patients
suffering from harmful gas poisoning should be treated
with HBO as early as possible. And HBO is one of the
necessary treatments to cope with harmful gas poisoning.
K154
A therapy effect analysis of hyperbaric oxygen
combination with method of blood-activating and
collateral-unblocking in treating patients with acute
cerebral infarction
Wang Xiaohong
Department of Rehabilitation and Physiotherapy,Wuhan
General Hospital of Guangzhou Command, Wuhan,
China
Objective: To investigate the clinical effects of hyperbaric
oxygen
(HBO)
combination
with
method
of
blood-activating and collateral-unblocking (BACU) on
patients with acute cerebral infarction (ACI) and to
analyze the mechanism of action. Methods: 84patients
suffered from ACI were randomly divided into HBO plus
BACU therapy group (HBO group) and BACU therapy
group (BACU group). The levels of serum endothelin
(ET-1), nitric oxide (NO) and the C-reactive protein (CRP)
were measured before and after treatment. The resultant
data were compared with normal healthy crowd. Results:
The levels of serum ET-1 and NO in patients with ACI
obviously elevated (P＜0.01). After treatment, the serum
ET-1 decreased, NO enhanced, and the ratio of ET-1/NO
decreased, while the decrease degree of the HBO group
was more remarkable, compared with the BACU group (P
＜0.01)At the same time, the CRP level in HBO group was
low significantly, compared with those before treatment (P
＜0.01), and no obvious change were observed in CRP
levels in patients before and after BACU therapy (P＞
0.05). Conclusions: Compared with solitary drug therapy,
HBO combination with BACU had better effect on ACI.
The main mechanisms of above combined therapy shows
that it could improve of vascular endothelium function,
inhibit the formation of ET-1, relieve vasospasm,
decrease in vivo inflammatory reaction, restrain blood
clotting, promote the establishment of collateral circulation,
and prevent ischemic brain tissue damage.
K155
The effect of hyperbaric oxygen treatment on the
plasm concentration of tPA, PAI-1 in the patients of
ischemic strok
1
2
2
2
Wei Wang , Lin Yang , Chunjin Gao , Baosen Pang
1. Department of Hyperbaric Oxygen, The Third Hospital
of Hebei Medical University, Shijiazhuang, China
2. Department of Hyperbaric Oxygen, Beijing Chao Yang
Hospital, Capital University of Medical Sciences, Beijing,
China
Objective: To investigate the effect of hyperbaric oxygen
treatment on the plasm concentration of tPA, PAI-1in the
patients of ischemic stroke. Methods: 137 patients with
acute ischemic stroke were divided randomly into routine
therapy group (routine group), hyperbaric oxygen therapy
group (HBO group). There were 27 healthy volunteers in
control group (control group). We measured the plasm
concentration of tPA, PAI-1 respectively in the patients
st
th
with ischemic stroke on the 1 and 11 day after
admission. Results: On the 1st day, in the patients of the
HBO group or of the routine group, the plasm
concentration of tPA and PAI-1 were significantly higher
than that of the control group (P>0.05); The tPA
concentration was lower in the HBO group compared with
the routine group (PAfter the routine treatment, the plasm
concentration of tPA decreased (P<0.01); After HBO
treatment, the plasm concentration of tPA, PAI-1 both
decreased (P<0.01). Conclusion: In the patients of acute
ischemic stroke, hyperbaric oxygen treatment can
decrease the plasm level of tPA, PAI-1. Thus hyperbaric
oxygen treatment can effectively improve the function of
coagulation-fibrinolytic system in the patients with
ischemic stroke, and may ameliorate therapeutic effect.
K156
Clinical effective observation on combined hyperbaric
oxygen therapy for noxious gas poisoning
Shuzhen Wang, Yuqin Han, Yun Li, Hongling Yang,
Jianming Zhan
Qilu Hospital of Shan Dong University, Jinan, China
Objective: To
investigate
the
effect
of
combined hyperbaric oxygen therapy on noxious gas
(mainly on carbon monoxide) poisoning. Methods: 339
cases of carbon monoxide poisoning were treated by
hyperbaric oxygen, with the supplement of the medicine
(vasodilator, diuretic, neurotrophic medicine). On the base
treatment of air compression, patients were treated with
0.2 Mpa hyperbaric oxygenation (severe patients at 0.25
Mpa), wearing masks and inhaling pure oxygen for 20 min
and the air in chamber was inhaled for 5 min, repeating 3
times such inhalation, then decompressed. Twice HBO
therapy a day for severe patients, once a day after the
patients awake. For 10 time HBO as a period of treatment.
Results:
325
cases
were
cured,
becoming life-independent, 8 cases dead because of
failure to receive HBO treatment due to critical condition
and transient vital signs, and 6 cases of carbon monoxide
intoxication delayed encephalopathy. The total effective
rate is 95.8%. Conclusions: The combined hyperbaric
therapy for noxious gases poisoning is effective, with a
high cure rate, quick effect, less complication and low
mortality rate.
K157
Therapeutic effect of hyperbaric oxygen integrated
therapy on hydrocephalus of craniocerebral injury
Shengzhi Wang, Yu Peng
Binhai Hospital, Shengli Oil Administration, Dongying,
Shandong, China
Objective: To explore the curative effective of hyperbaric
oxygenation (HBO) combined with drugs therapy on
hydrocephalus of craniocerebral injury. Methods: Ten
pentients with hydrocephalus from craniocerebral injury
were treated with hyperbaric oxygen treatment which
given 0.23 MPa HBO exposure ,20 min × 4( oxygen ) + 5
min (air ) once daily for 1~6 courses all together and once
course was 10 days, basides drug`s treatment of citicoline
sodium injection and adenosine disodium triphosphate
injection al. to observe and evaluate the clinical changes
and hydrocephalus change on CT. Results: Ten
pentients with hydrocephalus from craniocerebral injury
improved significantly clinical symptoms and signs after
3~10 days, the patients clinical symptoms ,signs and
hydrocephalus on CT almostly disappeard after 30 days
`treatment. Some pentients went on treating for 60 days
and all cured. Conclusions: Hyperbaric oxygen
integrated therapy may promotly repair the circulatory
system of cerebrospinal fluid in craniocerebral injury`s
pentients and hydrocephalus were gradually insorpted or
got into the circulatory system of cerebrospinal fluid, thus
controled hydrocephalus growth. It must be treated the
pentients on hydrocephalus of craniocerebral injury with
HBO at the right time, in order to increase the therapeutic
effect.
K158
The effect of hyperharic oxygen on D-dimer and
fibrinogen in the blood plasma of ischemic apoplexy
patients
1
1
2
Qiusha Wang ,
Shizhong Wang ,
Chunjin Gao ,
1
1
1
1
Qihua Yang , Jin Mei , Ping Zhang , Jianding Gu
1. Department of HBO, The 2nd People’s Hospital of
Changzhou, Affiliated Hospital of Nanjing Medical
University, Changzhou, China,
2. Departmen of Hyperbaric Medicine, Beijing Chaoyang
Hospital, Beijing, China
Objective: To observe the effect of hyperbaric oxygen
(HBO) on D-dimer (D-D) and fibrinogen (Fg) in the blood
plasma of ischemic apoplexy patients. Methods:
Ischemic apoplexy patients were randomly divided into
routine treatment group (97 cases), HBO treatment group
(137 cases), and healthy control group (40 cases) was
setup. D-D and Fg levels in blood plasma were detected
by coagulometer of the STAGO Company and its
corresponding kit. Results: D-D and Fg levels in the
blood plasma of the routine treatment group were higher
than those of from the control group when hospitalized
(P<0.01, P<0.05). D-D kept increasing on the 10 th day,
after admission (P<0.01); while Fg was lower than that of
the cortrol group (P<0.01). On the 20thday, D-D started to
decrease. However, it was still higher than normal level
(P<0.05); while Fg becomed normal, which showed no
significant difference from that in the control group
(P>0.05); D-D and Fg levels in the blood plasma of the
HBO group were higher than those of the control group
when hospitalized (P<0.01,P<0.05). D-D kept increasing
on the 10thday after admission (P<0.01). However, it was
still lower than that of the routine treatment group
(P<0.05); while Fg started to decrease, but it was higher
than normal level (P<0.05). On the 20th day, D-D and Fg
levels showed no significant difference from those of the
control group (P>0.05). Conclusions: HBO can relieve
coagulation, anticoagulation and abnormal fribrinolysis
due to ischemia and damage in the vessels through
regulation of D-D and Fg levels in blood plasma, which
was effective in preventing treating and ischemic
apoplexy.
K159
Effect of hyperbaric oxygen on iron deficiency in
patients with sensorineural deafiness
1
2
2
qiusha wang ,
shuchang tian ,
aihua sun ,
2
3
1
1
baodong wang , fuqiong pan , haian yu , shizhong wang ,
4
xuejun sun
1. Department of Hyperbaric Oxygen, the 2nd People’s
Hospital of Changzhou, Affiliated Hospital of Nanjing
Medical University, China
2. Department of Otorhinolaryngology, Shanghai
Changzheng Hospital, China
3. Department of Medical Rehabilitation, the Peoples
Hospital of Sichuan Province, China
4. Department of Nautical Medicine, Faculty 0f Navy
Medicine, Second Military Medical University, Shanghai,
China
Objective: To evaluate the effect of hyperbaric oxygen
(HBO) therapy on iron defieiency in patients with
sensorineural deafness. Methods: Two hundred and fifty
three patients were randomly selecled from outpatients
and inpatients with sensorineural deafness and treated
with HBO therapy from Dec. 1996 to Dec. 2004. Serum
ferritin (SI) and hemoglobin (Hb) were measured before
treatment and after one and two courses of treatment. Hb
and SI were also measured in 250 people undergone
healthy check-up in 2005. Results: After two courses of
HBO therapy, better curative effect was obviously shown
in treating mild deafnes than in treating moderaie
deafness. Statistically significant difference in Hb and SI
was observed before treatlment and after one course of
treatment, also after one course of treatment and after two
courses of treatment, respectively (P<0.05, P<0.01).
There was no statistically significant difference in Hb
between patients after two courses of treatment and
healthy people (P>0.05), but there was a statistically
significant difference in SI between thern (P<0.05).
Conclusions: Hyperbaric oxygen therapy may adjust SI
and Hb in patients with sensorineural deafness.
K160
Application of the apparatus for eustachian tube
opening function (ETOF) measurement before the
hyperbaricoxygenation (HBO)
1
2
3
Qiusha Wang ,
Chunjin Gao ,
Peisong Wang ,
4
4
1
Yunjing Wang ,
Yuming Yang ,
Yuhong Zhang ,
1
1
1
Guoying Wu , Yi Zhou , Jing Cao
1. Department of Hyperbaric Oxygen, the 2nd People’s
Hospital of Changzhou, Affiliated Hospital of Nanjing
Medical University, China
2. Department of Hyperbaric Oxygen, Beijing Chaoyang
Haospital, Capital University of Medical Science, China
3. Department of Hyperbaric Oxygen, Medical school
Hospital Haospital of Qingdao University, China
4. Naval Medical Research Institute of CPLA Shanghai,
China
Objective: To monitor Eustachian tube opening function
with the application of measurement of sound conduction
before HBO. Methods: Eustachian tube opening function
were monitored in patients before HBO with a
newapparatus for ETOF measurement, model HY-100
applied with high frequency sound at 7KHZ.If at the first
time the patient’s Eustachian tube was not open, the
measurement would be done in the next time after actions
of swallow, Valsalva maneuver and mandible oscillation
had been did. The results of measurement were
classificated by four grades. The standard for HBO was
made by the grade and whether Eustachian tube is open.
Results: The patient’ Eustachian tube with grade four in
classification and failure in five times of continues action
during the measurement was regarded as it was not open
and the patient was not suitable for HBO. Conclusion:
There is an advanced, stable and reliable performance in
the newapparatus for ETOF measurement, themonitor
and classification of Eustachian tube opening function
prevents from barotrauma and keeps HBO in going well.
K161
Effect of hyperbaric oxygen combined therapy on
persistent vegetative state in 46 patients
Wang Min
Department of Rehabilitation of Longgang District Central
Hospital of Shenzhen, Shenzhen, China
Objective: hyperbaric oxygen combined therapy on
persistent vegetative state and explore the best
therapeutic regimen for it. Methods: Forty-six cases of
persistent vegetative state treated with hyperbaric oxygen
combined therapy from 1995 to 2006 were analyzed
retrospectively, including the effects of etiological factors
and age factors and treatment juncture on therapy. Result:
The excellence rate and the improvement rate were
significantly higher in cerebral trauma group than those in
no cerebral trauma group. And two groups in which the
patients began to receive treatment when became
persistent vegetative state for less 61 days recovered
better than the other one in which the patients began to
receive treatment when became persistent vegetative
state for more 60 days. The excellence rate and the
improvement rate were significantly higher in cases below
30 years than these in cases over 30 years. Conclusion:
The effectiveness of hyperbaric oxygen on the patients
with persistent vegetative state caused by cerebral
trauma and the patients who just begin to receive
hyperbaric oxygen therapy treatment when having been ill
for less 61 days is better. The effectiveness of therapy is
better in younger cases than that in old cases。
K162
Effect of hyperbaric oxygen on ultrastructure of liver
and kidney in rats after acute carbon monoxide
poisoning
Guozhong Wang, Chunjin Gao, Huan Ge
Department of Hyperbaric Oxygen, Beijing Chaoyang
Hospital, Capital University of Medical Sciences, Beijing,
China
Objective: Acute carbon monoxide poisoning (ACMP) is
likely to be the most common cause of poisoning
worldwide and often results in persistent damage to the
important organs such as brain, liver and kidney. Some
trials have definitively shown hyperbaric oxygen (HBO) as
the efficacious therapy for acute carbon monoxide
poisoning. The objectives of this article are to study the
protective
effect
of
hyperbaric
oxygen
on
the ultrastructure of liver and kidney in rats after acute
carbon monoxide Poisoning. Methods: The rats were
randomly divided into ACMP 1st day group, ACMP 5th
day group, ACMP 10th day group, ACMP15th day
group,HBO1st day group, HBO5th day group, HBO10th
day group, HBO15th day group and normal control group.
Model rats after acute carbon monoxide poisoning were
established. The ultrastructure changes of liver and
kidney in rats of all groups were observed by electron
microscope. Results: Slight mitochondria swelling, focal
vacant area and rupture of mitochondria crista was
observed in some liver cells of ACMP 1st day group. The
pathological changes became aggravating in liver cells of
ACMP 5th day group and ACMP 10th day group, and
alleviating in liver cells of ACMP 15th day group.
Compared with simultaneous ACMP group, the pathologic
changes of ultrastructure in liver cells of HBO groups were
significantly improved. From ACMP 1st day group to
ACMP 5th day group, the pathologic changes of
ultrastructure in kidney cells was ingravescent. Although
the pathologic changes of ACMP 10th day group and
ACMP 15th day group became alleviating, slight
mitochondria swelling was observed in some epithelial
cells, meantime interstitial edema and congestion
remained obvious. Compared with simultaneous ACMP
group, the pathologic changes of renal glomerulus and
tubule of HBO 1st group and HBO 5th group were
significantly improved. Slight mitochondria swelling was
observed in a few renal tubular epithelial cell of HBO10th
day group and HBO15th day group. Conclusion: Carbon
monoxide has the toxic effects of tissue hypoxia and
produces
various
systemic
and
neurological
complications. Early hyperbaric oxygen can alleviate
pathologic lesion of ultrastructure of liver and kidney in
rats after acute carbon monoxide poisoning, which have
significant protective effect on the liver and kidney.
clinical therapy effectually , the curative effect of HBO
have been assumed.
K163
Empiricad study about the expression of IGF-1 on
brain tissue injured by treatment of hyperbaric
oxygenation in rats after traumatic brain injury
Jinzhong Teng, Jianfeng Wen
The 94th Hospital of PLA, Nanchang, China
K165
Study of the medical security on the 140m heliox
saturation–166m excurssion diving
Qinglin Lian, Xuejun Sun
Department of Diving Medicine, Second Military Medical
University, Shanghai, China
Objective: To investigate the relationship between the
therapeutic effect of hyperbaric oxygenation on brain
injury and the cerebral expression of IGF-1. Methods:
Base upon the Feeney’s model, we set up the cerebral
trauma model in SD rats. The rats were randomly
assigned to four groups: sham operated group, Control,
trauma and normal pressure oxygenation treatment (NPO)
group, trauma and hyperbaric oxygenation treatment
(HBO). The cerebral expressions of IGF-1 were analyzed
with immunohistochemical technique. Results: After the
treatment of hyperbaric oxygen, the expression of IGF-1
in hyperbaric oxygen treatment group was higher than
normal pressure oxygen treatment group and surgical
trauma control group (P<0.01). Conclusion: The
expression of IGF-1 enhanced in nerve cells after brain
injury, and it enhanced more obviously under hyperbaric
oxygen treatment. It indicates that the treatment of
hyperbaric oxygen can protect and recover the impaired
nerve cells. Improved the expression of IGF-1 in brain
tissue cells might was one of the nerves protection ways.
Objective: In order to raise the salvageability of the naval,
cultivate the divers of the saturation deep diving and
cultivate the person of medical security and diving
equipment support. Methods: eight divers were exposed
to 140m He-O2 saturation pressure in the DCC for 48h,
the partial pressure of O2 was 40±2 kPa in the DCC.
During the saturation period, the divers the excursion
dives reached to a depth of 164～166m by means of the
SCC and effectively finished the work on the bottom. The
divers safely return normal pressure after 149 h 20 min
decompression. Base on the clinical examination, the
function of nerve, mentality, heart and blood vessels,
respiration, immunity, stress, motion balance and audition
of divers were tested in different phases. Results: At the
saturation-excursion period,
worry, anxiety and
depression of the divers were existed, the MVV of
pulmonary functions were significantly lower with the
diving depth, the accurate rate of the ball put test
decreased sharply, the levels of 17-OHCS, CA, A, NA,
ACTH, FSH, T, GH, and C were significantly higher.
Conclusions: In this successful diving, the medical
security was very effective and practical. The
physiological changes of the body were temporary and
may be recovered.
K164
Attendant experience of combination therapy
containing hyperbaric oxygen for fetal growth
restriction induced by severe preeclampsia
Yuhua Wang, Hongjian Liu
The people’s hospital of Cangzhou, Hebei, China
Objective: To explore the attendant means of
combination therapy containing hyperbaric oxygen(HBO)
for fetal growth restriction induced by severe
preeclampsia. Methods: 32 patients with fetal growth
retardation(FGR) induced by severe preeclampsia,
treated by HBO combined with routive methods .treatment
Pressure was 0.15 Mpa,Qd; Continnal therapy times is
five. Results: in all 32 patients, the prominent effective
ratio was 59.4%, the effective ratio was 100%, and the
existent ratio of newborn baby was 94.1%. By
strengthening psychology nursing and special nursing of
the boodle pregnant woman, no one side effect was found.
Oxygen –toxicosis were not discovered in the newborn
babies observed two years later. Conclusions: By
improving blood oxygen-content and blood oxygen
dispersive capability, so the placental microcirculation
improved significantly, lacking oxygen of fetus
corrected ,the live rate of newborn babies improved
significantly. By mentality nursing timely and effective and
nursing in oxygen-store meticulously and attentively, the
HBO treatments to the boodle of pregnant women were
accomplished smoothly. Supporting the carry out of
K166
Mechanism of hyperbaric oxygen preconditioning in
neonatal hypoxia-ischemia rat model
Li Zhang, Wenwu Liu, Zhimin Kang, Xuejun Sun
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical University, Shanghai, China
Objective: We examined the mechanism of hyperbaric
oxygen preconditioning (HBO-PC) in neonatal HI rat
model. Methods: Seven-day-old rat pups were subjected
to left common carotid artery ligation and hypoxia (8%
oxygen at 37ºC ) for 90 min. HBO (100% O2, 2.5
atmospheres absolute for 2.5 h) were administered by
placing pups in a chamber 24 h before HI insult. Brain
injury was assessed by the survival rate, 2, 3,
5-triphenyltetrazolium chloride (TTC), Nissl, TUNEL
straining and caspase-3,caspase-9 activities after HI.
Results: In HBO preconditioned animals, survival rate
was increased, infarct ratio was decreased, and the
positive stained TUNEL cells was reduced, accompanied
by and suppression of caspase-3 and -9 activities.
Conclusion: These results indicate that a single HBO-PC
appears to provide brain protection against HI insult via
inhibition of neuronal apoptosis pathways.
K167
Hyperbaric
oxygen
pretreatment
accelerates
angiogenesis and alleviates myocardial ischemia in
rats
1
1
1
2
Cuihong Han , Li Lin , Qiang Sun , John H. Zhang ,
1
Xuejun Sun
1. Department of Diving Medicine, Faculty of Naval
Medicine, Second Military Medical University, Shanghai,
200433, P. R. China.
2. Department of Physiology and Pharmacology, Loma
Linda University School of Medicine, Loma Linda,
California, USA
Objective: Hyperbaric oxygen (HBO) has been previously
shown to protect the heart from ischemia-reperfusion
injury. The present study aimed to test the hypothesis that
HBO pretreatment enhances neovascularization in
ischemic
myocardium.
Methods:
Adult
male
Sprague-Dawley rats were randomly divided into four
groups: normoxia + sham surgery (CS), normoxia +
permanent occlusion of the left anterior descending (LAD)
coronary artery (CMI), HBO pretreatment + sham surgery
(HS), HBO pretreatment + permanent LAD occlusion
(HMI). Rats receiving HBO pretreatment were
intermittently exposed to 100% O2 at 2.5 atmospheres
absolute (ATA) for 60min, twice daily for 2 days followed
by 12h of recovery in room air prior to the myocardial
ischemic insult induced by LAD ligation. Rats in the
normoxia group were time matched with the HBO group
and maintained under normoxic conditions prior to LAD
occlusion. At 3 and 7 days after LAD occlusion, heart
function parameters were measured by inserting a
catheter into the left ventricle, infarct size was calculated
using the method of TTC staining, myocardial capillary
density was determined by immunohistochemical staining
with a monoclonal anti-CD31/PECAM-1 antibody, VEGF
protein level was determined by Western blot analysis,
and cell proliferation was detected by BrdU staining.
Results: At 3 days after LAD ligation, the infarct size of the
HMI group was significantly smaller than that of the CMI
group (26±2.5% vs. 38±3%, Pmax and -dP/dtmax were
significantly improved in the HMI group compared to the
CMI group at 3 and 7 days after LAD occlusion. Capillary
density, VEGF protein levels, and cell proliferation
detected by BrdU staining were significantly increased in
the ischemic myocardium pre-exposed to HBO.
Conclusion: HBO pretreatment accelerates angiogenesis
and alleviates myocardial ischemia in the present rat
model.
K168
The affect of hyperbaric oxygen therapy on the
cerebral infarction
1
2
2
Wenli Zhang , Xiaojie Ding , Wei Wang
1. Thoracic hospital of Qingdao, China
2. Central hospital of Qingdao, China
Objective: To study the affect of hyperbaric oxygen
therapy on the cerebral infarction; Methods: In the
hyperbaric oxygen therapy group, 90 patients were
treated with hyperbolic oxygenation one time in ten days
besides the medicine treatment; Results: The affect of
hyperbaric oxygen therapy group on the cerebral
infarction was more than the medicine treatment group;
Conclusion: hyperbaric oxygen therapy could disease
the side effect of nerve damage, and prevent the recurrent
of the cerebral infarction.
K169
Hyperbaric
oxygen
preconditioning
promotes
angiogenesis in rat liver after partial hepatectomy
1
2
2
2
Ping Ren ,
Zhimin Kang ,
Guojun Gu ,
Yun Liu ,
2
2
3
2
Weigang Xu , Hengyi Tao , John Zhang , Xuejun Sun
1. Department of pharmacology, Chinese Pharmaceutical
University, Nanjing, China
2. Department of Diving Medicine, Faculty of Naval
Medicine, Second Military Medical University, Shanghai,
China
3. Department of Physiology and Pharmacology, Loma
Linda University School of Medicine, Loma Linda,
California, USA
Objective: Hyperbaric oxygen preconditioning (HBO-PC)
increases the level of HIF-1α and its target gene VEGF
which is involved in angiogenesis. Liver regeneration is an
angiogenesis-dependent process. We hypothesized that
HIF-1α and VEGF mediated the angiogenesis effect of
HBO-PC on regenerating rat liver. Methods: Male
Sprague Dawley rats received HBO-PC followed by 70%
partial hepatectomy. Proliferation of hepatocytes and
endothelial cells was evaluated by BrdU staining.
Microvascular
density
was
assessed
by
immunohistochemistry. mRNA expression of HIF-1α was
assessed by quantitative RT-PCR and protein levels of
HIF-1α and VEGF were assessed by western blot. HIF-1α
DNA-binding activity was determined with an
ELISA-based kit. Results: HBO-PC increased the
proliferation index of endothelial cells and microvascular
density at 48 hr after partial hepatectomy. The protein
level and DNA-binding activity of HIF-1α and the protein
level of VEGF were increased by HBO-PC before and
after partial hepatectomy. Partial hepatectomy alone also
increased proliferation index and the expressions of
HIF-1α and VEGF. Conclutions: Our results indicated
that the angiogenesis effect of HBO-PC on liver after
partial hepatectomy could be achieved by increased
HIF-1α activity and VEGF expression. However, the
angiogenic effect of HBO-PC is moderate and HBO-PC
failed to produce additional effect on the enhancement of
HIF-1α and VEGF induced by partial hepatectomy alone.
K170
Hydrogen therapy reduces apoptosis in neonatal
hypoxia-ischemia rat model
Jianmei Cai, Zhimin Kang, WenWu Liu, Xuejun Sun
Department of Diving Medicine, Faculty of Naval Medicine,
Second Military Medical University, Shanghai, China
Objective: Hydrogen gas has been used in medical
applications to prevent decompression sickness in deep
divers from safety profiles. Recently, Ohsawa et al found
that molecular hydrogen can selectively reduce •OH and
ONOO– in cell-free systems and exert a therapeutic
antioxidant activity, using a rat middle cerebral artery
occlusion model. But the mechanism involved in the
protective effects of Hydrogen therapy was unclear.
Hypoxia-ischemia brain injury is a major cause of
neuronal cell death especially apoptosis in the perinatal
period. This study was designated to examine the effect of
hydrogen therapy on apoptosis in an established neonatal
Hypoxia-ischemia
rat
pup
model.
Methods:
Seven-day-old rat pups were subjected to left common
carotid artery ligation and then 90 min hypoxia (8%
oxygen at 37 °C). Immediately after Hypoxia-ischemi a
insult, pups were placed into a chamber filled with 2%
Hydrogen for 30 min, 60 min, or 120 min, respectively. 24
hr after 2% Hydrogen therapy, the pups were decapitated
and
brain
injury
was
assessed
by
2,3,5-triphenyltetrazoliumchloride (TTC), Nissl, and
TUNEL staining, as well as caspase-3, caspase-12
activities in the cortex and hippocampus. Results:
Hydrogen treatment in a duration dependent manner
significantly reduced the number of positive TUNEL cells
and suppressed caspase-3 and -12 activities.
Conclusion:
these
results
indicated
Hydrogen
administration after Hypoxia-ischemia appeared to
provide brain protection via inhibition of neuronal
apoptosis.
K171
Hyperbaric oxygen treatment in nitrogen poisoning
Sun Lin, Guan Hongye
No.1 People’s Hospital of Foshan Guangdong Province,
China
Objective: Further understand the importance of
hyperbaric oxygen treatment in Nitrogen poisoning.
Actively cooperated with the clinical treatment, hyperbaric
oxygen can improve the survival rate. Methods: The
patient put on the PSA-controlled gas-breathing machine
and received treatment in high pressure hyperbaric
oxygen cabin. Increase pressure for 25 minutes and
pressure is 2.3 ATA. Breathing oxygen for 1 hour, then
take a 5-minute break and decrease the pressure for 20
minutes. Result: The patient recovered. Conclusion:
Hyperbaric oxygen is a fast and effective therapy of
saving acute hypoxia caused by Nitrogen Poisoning.
K172
The research of the cerebral anabiosis success under
hyperbaric oxygen
1
1
2
3
Li Sun , Yan Shi , Guoping Feng , Yonggang Tan
1. Hyperbaric Oxygen Department, Shengli Oilfield
Central Hospital, Shandong, China
2. Emergency Department, Shengli Oilfield Central
Hospital, Shandong, China
3. ICU room, Shengli Oilfield Central Hospital, Shandong,
China
It reported eighteen cases of operating cerebral anabiosis
under hyperbaric oxygen after cardiopulmonary anabiosis
success and discussed the factor of success. There are
fourteen cases of cerebral anabiosis success, two cases
of bequeathing persistent vegetative state, two cases of
death. The result of the treatise prompts that the operating
of hyperbaric oxygen therapy on early stage basing the
cardiopulmonary anabiosis success can improve the
success rate of the cerebral anabiosis.
K173
Psychological problems of the patients in hyperbaric
chamber and nursing methods
Song Juan
the 306th Hospital of P.L.A, Beijing, China
Objective: This thesis mainly studies the psychological
problems of the patients who are treated in the hyperbaric
chamber, and discuss the nursing methods of appeasing
the psychological problems of the patients. Methods:
Based on the different psychological problems of the
patients, we adopt the health education, organizing
informal discussions, nursing in the chamber and other
kinds of measures.
Results: Patients objectively get
some knowledge of hyperbaric oxygen treatment. They
also actively cooperate the treatment with a very stable
mood. Conclusion: Nurses should make a lot of progress
in the professional qualities and are aware of the
importance of accompanying the patients in the chamber.
K174
The detection and clinical significance of NSE level in
cerebral infarction patients’ serum with hyperbaric
oxygen treatment
Hansheng Sheng, Hongbo Liu
Zhu Hai People’S Hospital, China
This paper is to study serum NSE level in cerebral
infarction patients with hyperbaric oxygen treatment. The
more NSE, the more it effusing from cells into
cerebrospinal fluid and blood, combined with the changes
of the cerebral blood flow metabolism, due to cerebral
edema, which can cause the secondary lesion in century
nervous system and aggravate the deterioration of the
function of the blood-brain barrier. The NSE level after
cerebral infarction if positive related to the pathogenetic
condition. Therefor contrasting the NES level after pure
oxygen and mixture hyperbaric oxygen treatment can help
to predict severity and outcome of cerebral infarction. It
provides evidence that Hyperbaric oxygen treatment can
improve cerebral anoxia, lower intracranial pressure,
increase the partial pressure of oxygen of reticular
activating system and brain stem, thus reduce the NES,
then promote consciousness recovering, improve the
clinic symptom eventually.
K175
Clinical value of HBO and drug comprehensive treat
in chronic appendicitis
Jing-yi SHAO,
Yan-bin ZHANG,
Zi-yang CAO,
Zhi-hong XIU, Pei-jin Lu
Liaocheng Second People’s Hospital Affiliated to Taishan
Medical University, China
Purpose: Inquiry the clinical value that the hyperbaric
oxygen (HBO) with drug in treating chronic appendicitis.
Method: 116 chronic appendicitis patients is divided into
treating set and matched set random, the treating set
treated with HBO and drug comprehensive, the matched
set with applied drug only. Results: Treat set 58 cases,
recovery 47 (81.0%), valid 9 (15.5%), invalid 2 (3.5%),
total efficiency 96.5%；Matched set 58 cases, recovery 35
(60.3%), valid 16 (27.6%), invalid 7 (12.1%), total
efficiency 87.9%.Two sets of total efficiency have shown
the relatively difference (p<0.01). Conclusion: HBO with
drug comprehensive treatment in chronic appendicitis has
the relatively effect, and has important clinical application
value.
K176
Interfering effect of hyperbaric oxygen on the patient
of cerebral tumor and cerebral artery tumor after
operation
Cunge Ren, Fen Wei, Futing Ma
The Affiliated Hospital of AirForce Aviation Medical
Research Institute of PLA, Beijing, China
Objective: To approach the interfering effect of
hyperbaric oxygen on the patient of cerebral tumor and
cerebral artery tumor after operation. Methods: 30
patients of cerebral tumor and cerebral artery tumor after
operation were divided equal 2 group by the method of
semi-stochastic, the hyperbaric oxygen exposure group
and the control group. The former group was cured by
hyperbaric oxygen based on routine method, the
later were only carried through injection of nerve-nutrition
and sustain drug. Results: The therapy effect of 2 group
have obvious distinct in directional thought, daily behavior,
improve of the remembrance, the ability of self-control
(P<0.05). Conclusion: The therapy of hyperbaric oxygen
are advantaged in nerve tissue rehab, eliminated the
oppressed symptoms of nerve tissue, reduce the
sequela etc. It can shorten the time window of miss the
opportunity of nerve function obviously, and improve the
life quality of patient.
K177
Hyperbaric Oxygen Therapy Nursing of Patients with
Tracheotomy
Qian Chunling, Wang Neng
Chizhou People’s Hospital, Anhui, China
Objective: To analyze the medical safety and effect of
hyperbaric oxygen (HBO) therapy in patients with
tracheotomy. Methods: The paper analyzed the situation
of the50 patients treated with hyperbaric oxygen, holding
that three tasks should be done to improve the treatment
level: to prepare oxygen cabin and nursing articles, to
nurse carefully, for example: general nursing, oxygen
inhale, sputum suction and observation on patients illness
and vein transfusion, to improve the level of nursing care
and enhance their sense of responsibility. Results: The
treatment of patients was not broken off, and no
complication happened. Conclusion: It is key factor for
patients with tracheotomy to keeping breathe a lucid
before hyperbaric oxygen therapy, middle and after that.
K178
Observation of curative effect of hyperbaric oxygen
therapy on 3 cases of severe trauma patients in
wenchuan earthquake
Qi Yan
the Second Provencal People’s Hospital of Guangdong ,
Guangzhou, China
Objective: TO observe the curative effect of hyperbaric
oxygen on 3 cases of severe trauma patients in
Wenchuan earthquake. Method: Besides conventional
therapy, such as support therapy, anti-inflammatory
treatment and anticoagulant therapy, the 3 cases are
treated by HBO per day; 15 days are 1 course of
treatment. To observe the curative effect of hyperbaric
oxygen after 1 day, 3 days and 7 days, respectively.
Result: The symptoms of dizziness and headache of the
3 cases have disappeared, The quality of sleep and
emotion have been improved, the hematoma has been
obviously absorbed, the wound has healed well, The
sensory function has recovered gradually, the muscle
strength has been increased above 1 grade, which total
effective rate is 100 percent. Conclusion: It is effective
and safe for the severe trauma patients in earthquake
early to obtain hyperbaric oxygen therapy on the basis of
conventional therapy.
K179
The Influence of Anxiety on the Effect of Sudden
Sensorineural Hearing Loss
Jiang Yan, Wang Rongzhen, Chen Lijuan, Ding Xiaohong,
Lu Wei
Dept. of HBO, First Affiliated Hospital of College of
Medicine, Zhejiang University
Objective: To study the influence of anxiety on the effect
of sudden sensorineural hearing loss. Methods: 80 cases
were divided into two groups according to anxiety scores.
Group 1 had 57 cases, anxiety scores was more than 40.
Group 2 had 23 cases, anxiety scores was less than or
equal to 40. All the cases were treated by sympathetic
method of medicines combined with hyperbaric
oxygenation. Result: The general effective rate of the two
groups was 53% and 85% respectively, with significant
difference (p<0.05)．Conclusion：
：Anxiety has significant
influence on the curative effect of sudden sensorineural
hearing loss．
K180
The clinic experience of Hyperbaric oxygen in
Epilepsy Research Summary and Recommendations
1
2
Jinxiang Xie , Yongzhong He
1. Shantou Central Hospital Affiliated Shantou Hospital of
YAT-SEN University, China
2. Department of pediatrics, Longhu Peoples Hospital,
Shantou, China
Aims: The purpose of this study is to sum up the clinic
experience of Hyperbaric oxygen (HBO) in Epilepsy (EP)
and
make
some
sound
recommendations.
Methods: Several aspects, including means and main
points of therapy, use of anti- epilepsy drugs (AEDs) and
electroencephalograph (EEG), evaluation and affected
factors of curative effect , of Clinic data about HBO in EP
ware
analyzed
and
concluded.
Results:
Recommendations: ①Theory and practice research of
HBO in EP should be enhanced. ②Better therapeutic
process of HBO in EP should be explored persistently.③
EP can be considered as one of the diseases treated by
HBO. Sound curative effect of HBO in EP can be obtained
according to choosing subjects correctly, seizing the
curative time, working properly, using EEG and AEDs.
Conclusion: Hyperbaric oxygenation therapy (HBOT) in
EP has its own merits, such as short period of treatment,
sound curative effect, no obvious side effect. Therefore,
HBOT is a new and secure kind of technique and it can be
used extensively. Affirmatively curative effect of HBO in
most clients with EP can be achieved and its effect mainly
lies on the results of the primary diseases.
K181
Hyperbaric
oxygen
preconditioning
reduces
ischemic-reperfusion injury by inhibition of apoptosis
in a middle cerebral artery occlusion rat model
1
2
1
1
Jiasi Li , Zhi-min Kang , Su-ju Ding , Wen-wu Liu ,
3
1
John Zhang , Xue-jun Sun
1. Department of Diving Medicine, Faculty of Naval
Medicine, Second Military Medical University, Shanghai,
China
2. Changhai Hospital, Second Military Medical University,
Shanghai, China
3. Department of Physiology and Pharmacology, Loma
Linda University School of Medicine, Loma Linda,
California, USA
Objective: This study examined the mechanisms of
hyperbaric oxygen preconditioning (HBO-PC) on ischemic
reperfusion brain injury. Methods: Male Sprague-Dawley
rats (250-280g, n=144) were divided into control, middle
cerebral artery occlusion (MCAO) for 90 min, and MCAO
plus HBO-PC groups. HBO-PC was conducted four times
by given 100% oxygen at 2.5 atmosphere absolute (ATA) ,
for 1 hour at every 12 hour interval for 2 days. At 24 hour
after the last HBO-PC, MCAO was performed and at 24
hour after MCAO, mortality, neurological function, brain
water content, infarct volume, and cell death were
evaluated. Enzymatic activity of capase-3 and -9,
expressions of cytochourome c, Bcl-2 and Bax proteins
were performed in the samples from hippocampus,
ischemic penumbra and core, respectively. Results:
HBO-PC reduced brain edema, decreased infarction
volume, suppressed mortality, and improved neurological
recovery. HBO-PC decreased caspase enzyme activities,
upregulated BCl2 expression, and reduced cytoplasm
cytochourome C levels. Conclusions: HBO-PC protects
brain tissues from ischemic reperfusion injury by
suppressing mitochondrial apoptotic pathways.
K182
Clinical observation of hyperbaric oxygen and drug
treatment on facial neuritis
WEI Fen, MA Futing, REN Cunge
Affiliated Hospital of Institute of Aviation Medicine of Air
Force, Beijing, China
Objective: To observe the clinic curative effect of
hyperbaric oxygen and drug treatment on facial neuritis.
Methods: The subjects included 45 patients, which were
randomly divided into two groups: treatment group (n1=21,
drug therapy plus hyperbaric oxygen, 0.2MPa
99.5%oxygen, oxygen inhalation60min, 10days as one
course) and control group (n2=24, drug therapy:
prednisone 20-30mg/d po, and given vitB1 100mg+vitB12
500ug, take intramusular injection for 14days). Results:
Comparing the changes of clinical symptoms after one
course of treatment, there were notable difference in the
curative rate (91.7% vs 84.2%) between treatment group
and control group. The therapeutic effect of the treated
group is better than that of the control group (P<0.05).
Conclusions: Early HBO and drug therapy can shorten
the course and raise the cure rate of facial neuritis.
K183
The discussion on static-preventate -discharge
technologyfor single human hyperbaric oxygen
chamber
Jianhua Yang, Yan He, Caijun Mei
Affiliated Hospital to Chuanbei Medical University,
Sichuan, China
Objective: This paper analyses and explains the
objective properties on HBOC static electricity existence
so that inspire the manufacturer of single human
hyperbaric oxygen chamber pay more attention to
possible static harm in design, manufacture and install.
Methods: We discuss the static-preventate -discharge
technology about the manned HBOC vessel with the aids
of some directed or indirected static electricity
experiments and some actual circumstances of the
three-variety single human hyperbaric oxygen chamber in
our hospital. Results: The static-preventate -discharge
technology for the human-body or the equipment units in
the HBOC have feasibility and are practical. Conclusion:
The static-preventate -discharge technology for the
human-body or the equipment units in the HBOC need to
be done some researches and improved deeply so that
the safties are in the course of running the single human
HBOC at any time.
K184
Risk Management and Preventive Measures of
Hyperbaric Oxygen Chamber
YAN Xiaomin
The First Affiliated Hospital, Chongqing Medical University,
Chongqing, China
Objective: To discuss risk management and preventive
measures of hyperbaric oxygen chamber. Methods: To
identify and evaluate the risk factors of hyperbaric oxygen
therapy.
Results: preventive measures
for
risk
management of hyperbaric oxygen chamber were
constituted. Conclusions: To establish the holistic
operation regulations and implementation of risk
management of hyperbaric oxygen chamber are ultimate
guarantee for patient safety.
K185
Study on brainstem reflex in patients with primary
brain stem injury treated by hyperbaric oxygenation
integrated therapy
Lei Xue, Gangchuan Wu
Department of Hyperbaric Oxygenation, Nan Fang
Hospital, The Southern Medical University, Guang-zhou,
China
Objective: To investigate the predictive values of
brainstem reflex (BSR) on the evaluation of severity, the
effect and the prognosis in the patients with primary brain
stem injury treated by hyperbaric oxygenation (HBO)
integrated therapy. Methods: Sixty patients were divided
into two groups: HBO group (n=30) and control group
(n=30). HBO group were treated with HBO integrated
therapy. Control group were treated without HBO
conventional integrated treatment. During the treatment
BSR were monitored at once every day for finishing
treatments. The relationship for BSR ,Glasgow coma
scale (GCS) and Glasgow outcome scale (GOS) were
analyzed. Results: The BSR were closely related with
GCS and GOS. The lower of disappear-level of BSR,
GCS and GOS, the worse of the prognosis of the patients.
The death have 3 scores in GCS, BSR disappears on the
level of number Ⅳ～Ⅵ. The BSR were to be an index
used for adjusting the proposals of HBO treatment.
Conclusions: BSR were considered to be valuable to the
evaluation of severity, the effect and the prognosis in the
patients with primary brain stem injury treated by HBO
integrated therapy and to be an index used for adjusting
the proposals of HBO treatment.
K186
Monitoring effect of disinfection in hyperbaric oxygen
chamber and oxygen humidified tank and measures
of nosocomial infection control
Lei Xue, Xiaohui Liu
Department of hyperbaric 0xygenation, Nan fang hospital,
Southern medical university, Guang-zhou, China
Objective: To disinfect the air of hyperbaric oxygenation
(HBO) chamber, article surface of HBO chamber and
oxygen humidified tank and monitor their bacteria
counting and identification regularly. To improve the way
of disinfection with monitoring effect and control
nosocomial infection. Methods: The ways of disinfection
included cleaning up HBO chamber and ventilating by
electric fan, cleaning article surface of HBO chamber with
disinfectant and water, disinfecting HBO chamber with
disinfector for 30min~60min. Before HBO therapy and
after disinfecting of HBO therapy, the air of HBO chamber,
article surface of HBO chamber and oxygen humidified
fluid of oxygen humidified tank were sampled regularly.
The samples were analyzed with bacteria counting and
identification. The effect of disinfection was be used to
improve the way of disinfection control nosocomial
infection. Results: Our Disinfecting of HBO Therapy kept
the bacteria counting in the air of HBO chamber at
3
≤500cfu/m and on article surface of HBO chamber at
3
≤10cfu/m , fulfilled the requirement of class Ⅲ
environmental sanitation standard. The bacteria counting
in oxygen humidified fluid of oxygen humidified tank kept
3
at < 10 cfu/ml. Conclusions: Our Disinfecting Measures
of HBO therapy can controls nosocomial infection.
K187
The effect of combined hyperbaric oxygen on severe
head injury (report of 42 cases)
Zhihui Xie, Jianguo Li, Zuoxun Chen
The Affiliated Hospital of Zunyi Medical College, Guizhou,
China
Objective: To investigate the clinical effect of hyperbaric
oxygen (HBO) therapy on severe head injury. Method: 42
cases with severe head injury received combined HBO
and the effect was determined according to the clinical
curing criteria of diseases diagnosis. Results: Recovery
in 7 cases (16.7%),improvement in 30 cases (71.4%) and
inefficacy in 5 cases (11.9%),the total effective rate was
88.1%.The effect was better in the group of short course
than the group of long course, and it was significant
especially within twenty days(the total effective rate was
93.3～ 94.4%). The total effective rate of the children
below ten years old was higher obviously than that of the
adult (it was 100%,84.3% ～ 85.7% respectively).
Conclusions: As an effective treatment, HBO plays a
specific role in remedy of the acute and severe head injury.
HBO should be applied to the patients with severe head
injury as early as possible if their conditions allow.
Moreover, HBO has significant effect on severe head
injury in children compared with the adult; therefore, it
should be adopted early.
K188
An Investigation on hyperbaric oxygen in premature
infants with hypoxic-ischemic encephalopathy and
the long-term effect
1
2
Yongzhong He , Jinxiang Xie
1. Department of pediatrics, Longhu Peoples Hospital,
Shantou, China
2. Shantou Central Hospital, Affiliated Shantou Hospital of
YAT-SEN University, China
Aims: According to reviewing and summing up the
short-term and long-term effect of Hyperbaric Oxygen
Therapy in premature and underweight Infants with
Hypoxic-ischemic Encephalopathy (HIE), as well as the
experience, the purpose of this study is to recommend
some principles of Hyperbaric Oxygen Therapy.
Methods: Based on analyzing the clinic data and the
primary therapy, infant hyperbaric oxygen chamber was
used, the pressure was 0.13MPa, the content of oxygen is
between 72% and 75%, and once a day, 60min at a time,
9.6 times for every infant on average. Effect of just
terminating therapy and seven years old was assessed.
Results: All 33 infants recovered and leaved hospital
after Hyperbaric Oxygen Therapy. However, during the
observation, one died and five with serious HIE infants
had sequelas, the rest with serious HIE and all with slight
or moderate HIE recovered completely, the total efficiency
is 96.7 % (32/33). One infant in 32 has cerebral atrophy in
CT for brain and the other are normal. Side-effect such as
Broncho pulmonary dysplasia (BPD) and Retinopathy of
prematurity (ROP) has not been discovered. Conclusion:
According to the research of BPD and ROP made in
abroad, in principle, Hyperbaric Oxygen Therapy should
be forbidden in premature and underweight infants.
K189
A case of hyperbaric oxygen treatment causing
pneumatocephalus
Rong Xie, ZhiQiang Zhuang
PuNan Hospital, Shanghai, China
Objective: To approach the pneumatocephalusability
when hyperbaric oxygen treatment for acute brain injury
patient. Method: reporting a practicality. Result: a patient
suffered a acute brain injury and got left frontal bone
fracture. We cured him with hyperbaric oxygen. The
pressure of chamber is 0.12Mpa, the oxygen uptake time
is 30 minutes, then interrupt with 5 minutes air, and input
oxygen for another 30 minutes, the compression time 15
minutes, and lower to atmospheric pressure with 20
minutes. After 5 times of hyperbaric oxygen treatment,
head CT of the patient showed elliptical gas density in left
front brain. Conclusion: On the occasions of the head
lacerated wound, the fractured skull or skull bottom,
around the eye socket hypodermic and the conjunctiva
ecchymosis, the external auditory meatus or nasal
passage had the bloodstain, cerebrospinal fluid leaks
from nose, ear or eye. malfunction of smell, the vision to
be fuzzy, and symptoms of oculomotorius damage. It is
necessary to take a head CT before making the
hyperbaric oxygen treatment. If the patient shows some
symptoms of headache, dizziness, diplopia or vomit in the
pressure chamber. the doctor should stop compress,
respire oxygen and reduce pressure slowly. Then
reexamine head CT, to prevent the gas from entering
encephalocoele and cause pneumatocephalus.
K190
The first to enter is last to exit law of gas saturation
and de-saturation
Xiao Pingtian
Xiangya Hospital of Centre South University, Changsha,
China
objective: To investigate the law of gas getting into or out
of the body during changes of environmental gas pressure.
Method: We used two methods of cabin operation to
compare the region of variation of real efficiency figures of
partial oxygen pressure (PO2T) and partial nitrogen
pressure (PN2T) in five kinds of hypothetical tissues, and
with different compression time and different steady
pressure: In procedure I, patients were assigned to inspire
air during the compression stage; In procedure II, the
patients inspired oxygen during the compression stage.
Results: ①The PO2T of procedure II was markedly larger
than that of procedure I and the PN2T of procedure II was
markedly less than that of procedure I；②The size of
region of variation of PO2T and PN2T in both procedures
was in direct proportion to the speed of gas saturation of
the tissues；③The PO2T and PN2T of all groups increased
as the steady pressure increased. The PN2Tof procedure
I of all group increased on lengthening the time of
compression, but the PO2T was not increased, and, on
the contrary, it reduced a little. Conclusion: The law of
gas going into or out of the body is that the gas that is
saturated first, is the last to de-saturate (first to enter is
last to exit), and this law is mainly to increase the time
efficiency of hyperbaric effect. The law of “first rapid, later
slow” is to raise the pressure efficiency of gas saturated in
advance of hyperbaric effect. These two laws are the
significant theoretical basis for hyperbaric oxygenation
pretreatment and the reason that procedure II is selected
to replace procedure I. However, when procedure II is
applied, the compression time should not be overly
prolonged.
K191
The effect of hyperbaric oxygen on consciousness
recovery of the coma patients suffering from brain
ischemia re-perfusion
Xiao Pingtian
Xiangya Hospital of Centre South University, Changsha,
China
Objective: To investigate the effect of hyperbaric oxygen
on consciousness recovery of the coma patients who
suffer from brain ischemia re-perfusion. Method: 11
cases of coma patients suffering from brain ischemia
re-perfusion were recruited in this study. The clinical data
of the treatment of hyperbaric oxygen were collected and
analyzed. Results: 1) all five subjects with full recovery
received hyperbaric oxygen treatment within 60 hours
after coma; 2) There were two cases with little intelligence
damage. One patient were treated with hyperbaric oxygen
five 5 hours after coma. The other one received
sub-hypothermia treatment immediately after the
occurrence of cardiac arrest, and started hyperbaric
oxygen therapy at 21st day. 3) One subject receiving
hyperbaric oxygen three days after brain ischemia coma
was in persistent vegetative state. Another patient starting
hyperbaric oxygen four days after coma died finally.
Conclusion: The prognosis of the coma patients suffering
from brain ischemia re-perfusion is related to many factors.
An appropriate treatment opportunity is critical to the
protective effects of hyperbaric oxygen on brain cells in
the coma patients suffering from brain ischemia
re-perfusion.
K192
Safe oxygen inhaling device without resistance in
hyperbaric oxygen
Wu Zhide
Xiangya Hospital, Zhongnan University, Changsha, China
Objective: To evaluate the clinical value of a new oxygen
inhaling device by observing the oxygen inhaling
resistance and oxygen concentration in chamber. Method:
To divide into three groups: pulmonary oxygen supply
apparatus (A group), continuous oxygen supply apparatus
(B group), and new oxygen inhaling apparatus (C group).
Oxygen inhaling resistance and oxygen concentration in
chamber of three groups were measured. Result: there
was significant difference for oxygen concentration in
chamber in three groups (P<0.05), B group was obvious
higher than A group and C group(P ＜ 0.01), But no
significant difference was found between group A and
group C(P＞0.05); A group was obvious higher than B
group and C group in oxygen inhaling resistance(P＜0.01),
But no significant difference was found between group B
and group C(P＞0.05). Conclusion: New oxygen inhaling
device can diminish the oxygen inhaling resistance and
cut down oxygen concentration in chamber, in favor of the
treatment and safety of hyperbaric oxygen, then to be
worth further clinically generalization.
K193
Hyperbaric oxygen for acute myelitis----one case
report
Haibin Xiang, Rong Xie
Shanghai Punan Hospital, Shanghai, China
A 8-year-old girl entered the hospital with the chief
complaint “Lower limbs fatigue, following walking
dysfunction and the obstacles of urination and
defecation ”. PE: Disapear of PTR and ATR tests. Muscle
strain declined, and the muscle force of the lower limbs
were of grade 0~I. The Babinski’s signs of the lower limbs
were negative. The cerebrospinal test indicated the
corruption of blood brain barrier (BBB), the dispropotion
increased of IgG. And the MRI of the spine revealed:
abnormal signal of spinal cord (T4~T11), advisement
diagnosis was acute myelitis. Then the patient was given
high-dose hormone implusion treatment, associated with
neurotrophic drugs. Three days later, the patient could
urinate by herself. On the ninth day, the patient was noted
to have pathologic signs of neurosystem and can defecate
herself. But her lower limbs were still disability. Ten days
after the patient entered the hospital, she took the oxygen
treatment. One oxygen chamber session finished, the
patient’s muscle force of lower limbs increased to grade
Ⅲ－Ⅳ and the muscle strain also improved. PTR test
exist. She can walk by help. When two oxygen chamber
sessions finished, the patient recovered, she can walk by
herself. The PTR test performed showed normal exist, the
muscle force was grade Ⅳ—Ⅴ. This case the patient had
unsatisfactory effect under normal treatment, however,
combined with hyperbaric-oxygen treatment, the paralysis
gradually reduced, muscle force improved, and the patient
could walk without help. The hyperbaric-oxygen treatment
could improve the spinal oxygen saturation and the
pressure of spinal oxygen, increase the oxygen dispersion,
thus could improve the anoxic situation of spinal cord,
decrease the dropsy of spinal cord, and also improve the
function of BBB, alleviate the inflammational
conglutination of the tissue. The hyperbaric-oxygen
treatment could also extend the time of neurocell
regeneration; protect the reversible nerve, which in the
end improve the function of the nerve. Since the BBB
dysfunction could easily result in paralysis, we
recommended the use of hyperbaric-oxygen treatment
and especially use this remedy at the acute period of the
disease.
K194
The safe nurse of hyperbaric oxygen treatment on
moderate to severe craniocerebral injury patient
Wu Qiaoning
The Six Affiliated Hospital of GuangXi Medical University
Objective: To explore the preferred way of the safe nurse
of hyperbaric oxygen treatment on moderate to severe
craniocerebral injury patient. Methods: According to
clinical classification, pars affecta, associated injury,
operation, pathogenetic condition, complication and so
on,122 moderate to severe craniocerebral injury patients
were adopted correspondence safe nursing intervention
at prior hyperbaric oxygen treatment, during hyperbaric
oxygen treatment and post hyperbaric oxygen treatment.
Result: Because of safe nursing intervention is targeting,
all of the 122 moderate to severe craniocerebral injury
patients can complete hyperbaric oxygen treatment safety
and successfully, and there are no the complication and
accident of hyperbaric oxygen treatment. The curative
effect is satisfaction. Conclusion: Reinforcement on prior,
during and post hyperbaric oxygen treatment safe nursing
is the key point of treatment.
K195
Prevention and Treatment on the decompression
sickness of diving work fishermen
Wen Chengwen
People’s Hospital, Hainan, China
Objective: To study prevention and cure method and
treatment effect of the decompression sickness diving
work fisherman. Method: The object of treatment is
divided into patient’s team and health cure treatment set.
It is adopted dissimilar treatment project of the Chinese air
diving decompression sickness compression treatment
form. Result: The treatment efficient of patient team is
ninety porcent. The decompression sickness rate of
health cure treatment set is 0.67%. Conclusion: For the
fisherman of diving work, It should carry on compression
treatment periodical. For the patient of decompression
sickness, It should carry on compression treatment as
early as possible.
K196
Clinical observation of hyperbaric oxygen and
ginaton in the treatment of sudden deafness
Cuixia Xue, Xinli Zhang, Lin Wang
Department of hyperbaric oxygen, The First People’s
Hospital of Qingdao Economic and Technological
Development Area, Shandong, China
Objective: To investigate the effect of hyperbaric oxygen
(HBO) combined with ginaton for sudden deafness (SD).
Methods: 109 patients with sudden deafness were
divided into hyperbaric oxygen group and control group at
random. Both of the two groups were given ginaton. 57
patients with SD were exposed to HBO, which also were
divided into group A that was exposed to HBO in 3 days
after getting SD and b that after 3 days. 52 patients for
comparison (non-HBO group) were treated only by
ginaton without HBO. Results: The overall effective rate
in HBO group (84.2%) was higher than that in non-HBO
group (65.4%) (χ2=4.211, P<0.05). Furthermore, it in
group A (96.3%) was higher than that in group B (73.3%)
(χ2=4.041, P<0.05). Conclusion: Therapeutic effect of
HBO combined with ginaton for SD is significantly better
than that of simple ginaton without HBO.
K197
Case Study: A 56-year-old Man with acute methyl
chloroform saturation
Shi Chunmei
Shanghai Shuguang Hospital, Shanghai University of
Traditional Chinese Medcine and Pharmacology,
Shanghai
Clinical data: A 56-year-old man was inadvertently
exposure in methyl chloroform gas on Dec21st 2007,
immediately the patient felt headache and dizziness,
unsteady step like drunk, limb fatigue and numbness,
malaise and vomiting. The patient was seen in the
out-patient and given transfusion treatment. The tremedy
of transfusion was not clear. His symptoms were
controlled for a time. However, the headache and
dizziness increased, and he did not have a response to
treatment with transfusion, the patient was scheduled for
oxygen treatment on Dec27th 2007. The protocol of
hyperbaric-oxygen treatment was: exposed to 100
percent oxygen at 1 and then 2.2 atmospheres absolute
during the chamber session. After five times oxygen
chamber treatment, the patient recovered and remained
chest bored and limb fatigue. When one oxygen chamber
session finished, the patient was evaluated in the
out-patient clinic. Additional information were obtained, it
was learned that the chest bored disappeared, the
headach and dizziness decreased, and the limb
numbness also decreased. After two oxygen chamber
sessions, the symptoms of the patient dissapeared.
Discussion: Methyl chloroform was a kind of low toxity
poison. Reports on its poisoning mechanism on health
were poor. The approach for its poisoing was
predominately through aspiratory system. The target
apparatus of methy chloroform was neural system. When
exposure in low-concentration methyl chloroform, the
symptoms were restrain effects of neural system such as
body im-balanced. However, the exposure in
high-concentration methyl chloroform would lead to an
aesthesia-like symptoms of the neural system, even the
loss of respiratory and resulting in the death of patient. In
this case, the patient was inadvertently inhaled the methyl
chloroform gas during work, which resulting in the nerual
system symptoms such as headache and dizziness,
unsteady steps, limb fatigue, vomiting, chest boring and
weeping. All the clinical representation was consistent
with the data. The report of hyperbaric-oxygen treatment
on the methyl chloroform poisoning was very poor. This
case was gained satisfied effect by using oxygen chamber
treatment. The mechanism of it was need further
investigated.
K198
The Effects of the Hyperbaric Oxygen on
Consciousness Recover With Serious Brain Injury
1
2
2
Wu Qiong , Weng Qibiao , Wang Hongjun , Chen
2
2
3
Lingzhen , Chen Xuelin , ChenQiuYan
1. Taishan People’s Hospital of Jiangmen City,
Guangdong Province, China
2. Department of Hyperbaric oxygen, Zhujiang Hospital
attached to Southern Medical University, China
3. Nanhai People’s Hospital of Foshan City, Guangdong
Province, China
Aim: The effects of the Hyperbaric Oxygen on
consciousness recover with serious brain injury. Method:
Using single hyperbaric oxygen chamber to cure the
patients whose brains are seriously damaged before
losing their consciousness in 41 cases. Scheme: 0.2MPa
20min×2+40min.using the Hyperbaric oxygen chamber to
cure
the
patients
after
their
consciousness
recovered.Scheme:0.20Mpa 20min×2+90min. Result: 29
in 41 cases recoveredcure rate: 70.73%. Conclusion:
The Hyperbaric Oxygen is helpful in curing patients whose
brains are seriously damaged and it can reduce the rate of
persistent vegetated situation.
K199
The prognosis analysis of different hyperbaric
oxygen treatment period after cerebral hematoma
removal
Wen Li, Jinlong Sha, Haili Tang, Yan Yang, Hua Qiu
Dongguan Hospital, Dongguan, China
Objective: To investigate the effects of hyperbaric
oxygen treatment during the different period after
intracerebral hemorrahage removal. Methods: 73
postoperative patients after intracerebral hemorrahage
removal were divided into early treatment group (group A,
within 14days after operation, the patient number is 38,
Male:25, Female:13,age:28~70Y,average age:58Y) and
later treatment group (group B, more than 14days after
operation, the patient number is 35, Male:26, Female: 9,
age:30~69Y, average age:61Y) according to the
’
beginning time of therapy. Results: group A s efficient
’
rate is 94.73%, group B s efficient rate is 71.43%. To
compare the early treatment group with its counterpart,
there were significant difference (P < 0. 05) on the
improvement of therapeutic effect and daily living activity
between two groups. Conclusion: The therapeutic effect
of early treatment group is better than that of later
treatment group, The more early to start hyperbaric
oxygen treatment, the better prognosis we can get.
K200
Prevention and treatment of carbon monoxide
intoxication and tardus encephalopathy by HBO
Xia Huang, Guoyi Ren, Wenhui Li
Zhujiang Hospital, South Medical University, China
Objective: To investigate the therapeutic effect of
hyperbaric oxygen (HBO) on acute carbon monoxide
intoxication and the feasibility on prevention of tardus
encephalopathy. Methods: The clinical data of 46 cases
of moderate or severe acute carbon monoxide intoxication
treated with hyperbaric oxygen (HBO group) and 30 cases
of acute carbon monoxide intoxication with regular
treatment except HBO (control group) were analyzed.
Results: The effective rates were 97.83% for HBO group
and 76.67% for control group. There was a significant
difference between the two groups (p<0.05). The
incidences were 2.94% for HBO group and 13.33% for
control group. There was a significant difference between
the two groups (p<0.05). Conclusion: Hyperbaric oxygen
treatment can reach an obvious therapeutic effect on
acute carbon monoxide intoxication and reduce the
incidence of tardus encephalopathy.
K201
Clinical therapeutic effect of hyperbaric oxygen
treatment for craniocerebral injury
Quyun Yang, Qibiao Weng
Zhujiang Hospital, South Medical University, China
Objective: Value the Therapeutic effect hyperbaric
oxygen treatment for Craniocerebral Injury. Methods:
Totally 192 patients samples were enrolled, all the patient
s are randomly assigned to three groups. Group A (control
conventional therapy group). Group B (giving hyperbaric
oxygen treatment after Craniocerebral injury for 20 to 30
days) and gaining satisfied effect. Results: Stress
ulcerbleeding occur rate: There was significant difference
between group B and group A (P < 0.05) after
Craniocerebral Injury for 20 to 30 days. And no significant
difference between other groups (P < 0.05) on rest stage
and groups. Conclusion: Hyperbaric oxygen treatment
shows obvious curative effect for Craniocerebral Injury,
and treatment after injury for 20 to 30 days is the best
performance time. HBO is an important and active
measure in curing the Craniocerebral Injury.
K202
Investigate the effect observation and coordinate with
humanistic care nursing strategy of high-pressure
oxygen (HBO) treatment of persistent vegetative state
(PVS)
Wu Ruilan
HBO Center, Affiliated Hospital of Hainan Medical College,
Hainan, China
Objective: Investigate the effect observation and nursing
strategy of high-pressure oxygen (HBO) treatment of
persistent vegetative state (PVS) patients. Methods: 134
cases of PVS patients were randomly divided into two
groups: HBO treatment (n = 84) and conventional
treatment group (n = 50), testing PVS before and after the
treatment, and contrast with HBO after treatment efficacy
and duration, frequency, causes relations, coordinate with
humanistic care, observation of treatment. Results: The
total efficiency of HBO group is 85% and conventional
treatment group is 52%, respectively X2 = 16.59. P<0.01,
the effect of HBO treatment was significantly better than
conventional treatment group；compared to conventional
treatment before and after, the statistical difference was
no significant (P>0.05). Conclusion: HBO treatment to
PVS patients with a positive role in restoration, and have
closely related with the duration of treatment, frequency
and causes. The appropriate care during treatment, will
reduce the pressure of patients, and establish confidence
in the treatment and improve treatment.
K203
The effect of hyperbaric oxygenation therapy on the
flash electroretinogram in rats with experimentally
elevated intraocular pressure
Wu Shuanggui
Lengshuijiang People’s Hospital, Hunan, China
Objective:
To investigate
whether
hyperbaric
oxygenation therapy can improve the electroretinogram in
rats with experimentally elevated intraocular pressure
(IOP). Methods: Acute elevated IOP model was built in
each eye of 18 Wistar rats, then they were divided into A
and B groups randomly. Group A (no HBO group), nine
rats were not used any therapy. Group B (HBO group),
nine rats used hyperbaric oxygenation therapy after
model building. The electroretinogram was recorded
again after 7 days. Results: The b wave recoveries of the
electroretinogram in group A were (41.85±13.20)%; that in
group B were (60.04±13.20)%. The difference between
two groups was statistically significant (P<0.01).
Conclusions: Hyperbaric oxygenation therapy promoted
the FERG b wave recoveries. It indicated that HBO
therapy could protect retina on fighting against the
ischemia injures.
K204
Hyperbaric oxygen and traumatic brain injury
Yali Wu, Lianbi Xue
Hyperbaric Center of Beijing Tiantan Hospital, Capital
Medical University, Beijing, China
Objective：
：Traumatic brain injury (TBI) is called the silent
epidemic in china, because of which our society is now
suffering from heavy economic burden. All kinds of
medicine and hypothermia treatments have failed to
improve the TBI patients’ functional outcome and lower
down mortality rates. Recently, oxygen therapy has
shown a great effect in the animal and clinical research,
and it is quite promising to treat TBI with hyperbaric
oxygen (HBO) therapy. So the HBO therapy is getting
more and more important in the treatment of the acute TBI
and in the process of recovery. Consequently, this review
summarizes the current mechanisms of HBO treatment in
traumatic brain injury (TBI) and their efficacy. Methods:
Directed literature review. Results: It was considered that
the cerebral vasoconstriction and increasing the oxygen
utilization were the primary mechanisms of HBO in
treating TBI. But now, it is found that HBO can improve
the cellular level cerebral aerobic metabolism so as to
make the damaged mitochondrial recover. Furthermore,
HBO, with the ideal treatment paradigm of 1.5 ATA during
60 minutes, does not produce oxygen toxicity so the
patients will be relatively safe. However, although whether
to use HBO to treat TBI, remains controversial, more and
more evidences show that HBO may be a method to treat
the patients with brain injury. So further investigations are
required to prove the effect of HBO in treating TBI and the
pharmacy and treatment opportunity still need research.
K205
Hyperbaric oxygen treatment and nursing of 33 cases
of sudden deafness
Wu Yanqiu
Henan Provincial peoples Hospital in Zhumadian City
Centre, China
Objective: Discussion HBO on the effect of sudden
deafness observation and influence. Method: the 33
cases 1Outstanding deafness by age less than 50 fears
old and is divided into more than 50-years old with the
effect of observation. 2Checks can be divided into mild,
moderate and severe when the effect of HBO. 3After
treatment from the disease sooner or late can be divided
into less than a week and less than tree months, more
than three months to six months when the effect of six
months. Sudden deafness hyperbaric oxygen treatment
efficacy significantly. Results: According to the incidence
of different ages and the length and pure-tone audiometry,
the clinical course of the 3 - 4 separate high-pressure
oxygen, treatment, younger, the higher the cure rate and
efficiency, the shorter the onset time, the cure rate and
more efficient, pure-tone audiometry, classification, the
more light deafness, the higher the cure rate and efficient.
Conclusion: HBO treat of sudden deafness the clinical
efficacy and age, the incidence of time and the severity of
the illness. Middle-aged incidence after treatment sooner,
the best effect.
K206
Analysis of the therapeutic effect of low molecular
weight heparin sodium combined with hyperbaric
oxygen in patients with acute cerebral infarction.
Lianhua Wu, Chunjin Gao, Huan Ge, Guozhong Wang,
Liming Zhao, Yu Gao, Yi Zhang, Linlin Ma, Fujia Liu
Department of Hyperbaric Oxygen, Chaoyang hospital
Affiliated of Capital University of Medical Sciences Beijing,
China
Objective: To investigate the therapeutic effect of low
molecular weight heparin (LMWH) combined with
hyperbaric oxygen (HBO) on Patients suffered from acute
cerebral infarction. Method: 203 patients with acute brain
infarcted were divided in to 2 groups firstly. 101patents in
control group, were treated by0.1ml/10kg LMWH Sodium，
hypodermic injection, once per 12 hours, treatment begun
in 72 hours after acute brain infarcted. for a treatment
course of 7 days, There were102patents in the HBO
group, were treated with LMWH Sodium combined with
HBO witch was once a day for 20days.Neurological
impairment scores (NIS) and ability of daily life (ADL)
scores (Barthel index)were used as effective parameters
before and after treatment in two groups .Results: The
NIS was significant lower in HBO group than in control
group (p<0.01)and ADL score of the HBO group was
higher than control group(p<0.05) after treatments.
Conclusion: Low Molecular Weight Heparin combined
with Hyperbaric Oxygen has more therapeutic effect than
Low Molecular Weight Heparin in treating patients of
acute cerebral infarction.
K207
Clinical study on the relate markers of blood
coagulation in the patients with avascular necrosis of
the femoral head after severe acute respiratory
syndrome
lianhua wu
chunjin gao
guozhong wang
lin yang
xiaomin hou huan ge chengqing xia man qi
Department of Hyperbic Oxygen ,Chaoyang hospital
Affiliated of Capital University of Medical Sciences Beijing
China
Hyperbaric Oxygen Department,
People’s Hospital, China
Objective: To investigate the blood coagulation function
in the patients with avascular necrosis of the femoral head
after severe acute respiratory syndrome. Method: we
measured respectively the expression of CD 31, CD61,
CD 62p, CD63 and PAC-1 on platelet membrane by
flowcytometry, and measured the plasma prothrombin
time (PT), activated partial thromboplastin time (APTT),
thrombin time (TT) ,and fibrinogen (Fbg) by blood clotting
instrument in 26 patients with avascular necrosis of the
femoral head after severe acute respiratory syndrome and
in 17 healthy adults. Results: The expression of CD31,
CD61, CD 62p, CD63 and PAC-1 on platelet membrane in
26 patients were all lower than those in 17 healthy adults
(p0.05) in those markers between patients and 17 healthy
adults. Conclusion: The blood may not be in
hypercoagulable state in patients with avascular necrosis
of the femoral head after SARS.
ISO
refers
to
International
Organization
for
Standardization. The essence of standardization is the
design and implementation of all kinds of rules and
regulations. The design and implementation of rules are
absolutely necessary for treatment with hyperbaric
oxygen chamber. We can assure absolute safety of
hyperbaric oxygen chamber in treatment process,
increase the overall quality of the Hyperbaric Oxygen
Department, increase the safety management level for
hyperbaric oxygen chamber, improve all of the rules and
regulations in the process of modification and assure that
the safety management of hyperbaric oxygen chamber
keeps at the best level only when the designed rules and
regulations are strictly obeyed and firmly implemented to
turn visible rules into invisible actions. ISO standardized
management is the top priority in the treatment process
with hyperbaric oxygen chamber as it is directly related
with the life safeties of patients. Absolute life safeties of
patients who are treated with treated with hyperbaric
oxygen chamber can be assured only when all hidden
dangers are eliminated. The key to the safety
management of hyperbaric oxygen chamber lies on the
implementation as well as continued modification and
perfection of all sorts of rules and regulations.
Standardized management is extremely important in the
safety management of hyperbaric oxygen chamber.
K208
The role of hyperbaric oxygenation on the
convalescence of brain injury at high altitude
Hongzhi Xia, Suzhi Li, Chengliang Huang, Xuehong Yang,
Qing Zhang, Maoxu Wang
Department of hyperbaric oxygenation,Military general
hospital of Tibet, Lhasa, China
Aim: To evaluate the role of hyperbaric oxygenation
(HBO) on the convalescence of brain injury at high
altitude. Method: 270 patients convalescence of brain
injury treated with HBO adding to medicines compared
with 98 patients treated with medicines at the same period.
Result: The cure rate in HBO group was obviously higher
than that in medicine group. Conclusion: HBO can
ameliorative brain tissue anoxia quickly, increase contain
of oxygen, increase the oxygen save, make blood vessel
constringency of each organism, increase or reduce little
of sacral vertebrae iliac artery hem the discharge,
ameliorative reticular formation function of brain stem,
cerebella winning, benefiting to patients consciousness
instauration with wide awake, and can accelerate the
disease cooking stove the clearance, hematoma
absorption and make organize the inside gum quality the
cell to divide to increase in value, produce the large
quantity gum quality the fiber the tissue that repair the
harm, and establish the vice circulation, urge the
capillanisum regeneration quickly, promote the tapetum
tissue recovered.
K209
Standardized Management Based on ISO 9000
System is the Fundamental Assurance for Safety
Management of Hyperbaric Oxygen Chamber
Sun Li
Haikou
Municipal
K210
Effects of hyperbaric oxygen on the content of
inflammation mediators in the rat brain tissue
following fluid-percussive brain injury
1
2
1
2
Yue Li- na , Gao Chun-jin , Zhou Li-chun , Pang Bao-sen ,
2
2
Zhao Qi-hang , Wang Guo-zhong
1.Department
Of
Neurology,
Beijing
Chaoyang
Hospital,Capital University Of Medical Science, Beijing,
China
2.Department of Hyperbaric Medicine, Beijing Chaoyang
Hospital,Capital University Of Medical Science, Beijing,
China
Objective: To observe the changes of content of
interleukin-1beta(IL-1β),tumor necrosis factor- alpha
(TNF-α),Intercellular adhesion molecule-1(ICAM-1) and
interleukin-10 (IL-10) before and after hyperbaric oxygen
in the injuried brain tissue of Sprague-Dawley rats
following severe lateral fluid-percussive brain injury and to
explore the mechanism of hyperbaric oxygen on traumatic
brain injury. Methods: 72 male Sprague-Dawley rats were
randomly distributed into 3 different groups: traumatic
brain injury group (TBI group), hyperbaric oxygen group
(HBO group) and sham-operation group (SO group).The
severe traumatic brain injury models were established by
the fluid percussion device. After 6 to 72 hours of survival,
rats were killed, and about 200 mg of brain tissue that
contained the injury site, as well as tissue proximal to this
region,was isolated for homogenizing.The concentrations
of IL-1β, TNF-α, ICAM-1and IL-10 in the homogenate of
brain tissue were measured at 6h, 24h, 48h and 72h,
respectively, by using enzyme-linked immunosorbent
assay (ELISA).Protein levels were determined by the
method of Bradford. Results: were expressed as
means±stand deviation.The SPSS 11.5 software was
used for statistical analysis. The differences between
groups
were
compared
using
a
One-Way
ANOVA,followed by an inter-groups analysis using the
Student Newman Keuls Test. Differences were
considered significant if the P value was <0.05. Results:
1.The concentration of IL-1β in homogenate in the HBO
group at 6h was significantly higher than that of the SO
group (p<0.05), but lower than that of the TBI group
(p<0.05). The concentration of IL-1β in homogenate in the
HBO group at 24h was significantly lower than that of the
TBI group (p<0.05). 2. The concentration of TNF-α in
homogenate in the HBO group at 6h was significantly
lower than that of the TBI group (p<0.05). 3. The
concentration of ICAM-1 in homogenate in the HBO
group respectively at 6h, 24h, 48h and 72h was
significantly higher than that of the SO group (p<0.05),but
lower than that of the TBI group (p<0.05). 4.The
concentration of IL-10 in homogenate in the HBO group
respectively at 6h, 24h and 48h was significantly higher
than that of the SO group (p<0.05),but there was no
significant difference compared with TBI group (p>0.05).
Conclusions: 1. Fluid percussion device can establish a
severe traumatic brain injury model of rat successfully. 2.
IL-1β, TNF-α, ICAM-and 1IL-10, which took part in the
inflammatory mechanism after trauma, were elevated in
the injuried brain tissue following TBI. 3. HBO treatment
can effectively relieve the inflammatory following TBI
through inhibiting the expression of IL-1β, TNF-α and
ICAM-1, but can not throuth elevating the expression of
IL-10. HBO may play a role in preventing the secondry
brain injury and protecting the injury brain through the
above mechanisms.
First Author’s Index
A
Renu, Agnihotri OR08
Chavez, Alberto OR10
B
Jiaxiang, Bai K11
Dirk J., Bakker B1
Jian, Bao K64
John M, Batle OR11 P52
Michael, Bennett OR06
Zhumei, Bi K56
Jing, Bian K76
C
Jianmei, Cai K170
Jorge, Calderón OR12
Hao, Chen K62
Jianhui, Chen K123
Lijuan, Chen K61
Sailian, Chen K60
Yanhua, Chen P53
Yifei, Chen P15
Jincheng, Cheng OR21
Philippe, Colombani P25
Frans, Cronjé PL2
Cao, Cui K73
Yan, Cui K125
D
Congying, Deng K32
Jordi, Desola PL11
Yuqin, Du K59
Shutian, Duan K46
F
Wenqing, Fan K131
Lianfeng, Fei P14
John, Feldmeier OR02 P44
Jochen, Freier PL5
G
Chunjin, Gao P10
Ningqin, Gao K57
Xiudong, Gao K68
Yu, Gao P33 K55
Chaoming, Ge K54
Huan, Ge K53
Guojun, Gu K66
Guanghan, Guo K52
Junmei, Guo K51
Na, Guo K50
Stephen, Guthrie PL10
H
Michal, Hajek OR03
Christer, Hammarlund OR14
Cuihong, Han K167
Chunyang, He P20
Guiying, He P13
Xia, He K49
Yongzhong, He K188
Mingyan, Hei K48
Christtian, Heiden OR01
Xiaomin, Hou K47
Te-Chun, Hsia P38
Cheng-Pu, Hsieh P42
Guiping, Hu K79
Huijun, Hu K81
Shengli, Hu OR28
Lingling, Huang P18
Min, Huang K74
Xia, Huang K200
Xiang, Huang OR13
J
Mingyi, Ji K90
Yunping, Ji K89
Jianping, Jiang K88
Wenbo, Jin K86
K
Mahito, Kawashima PL1
Lei, Kong K84
Jacek, Kot OR05
Aihua, Kuang K83
L
Zhaopan, Lai K30 K34
Hui-Chieh, Lee P47
Changchun, Li K82
Chu, Li K58
Chunfang, Li K130
Dongjuan, Li K69
Hongyan, Li K80
Houcheng, Li K77 K78 K92
Jiasi, Li P08 K181
Jinsheng, Li P28
Li, Li K70
Lingfang, Li K124
Mingxing, Li P51
Peiyun, Li K132
Shouchao, Li P19
Shunyong, Li K24
Wen, Li K150 K199
Xianghui, Li P21
Yajuan, Li K20
Youmin, Li K16
Yuxian, Li K21
Zengmin, Li K15 K19
Zhang, Li K18
Zhicai, Li K09
Zhuo, Li OR17
Qinglin, Lian K165
Shaohua, Liang K22
Xiuqing, Liang K14
Mao-Tsun, Lin OR07
Shilong, Lin K12
Ying, Lin K13
Daodong, Liu K02
Fang, Liu P34
Fujia, Liu K03
Guangsen, Liu K10
Jinglong, Liu OR30
Min, Liu P30
Qingle, Liu OR09
Wencheng, Liu K04
Wenwu, Liu K104
Xinying, Liu K05
Xuehua, Liu OR25
Yaling, Liu K06
Yi, Liu K07
Ying, Long K35
Min, Lou OR20
Jiangjiang, Lu K44
Peigang, Lu P12 K45
Xiaoxin, Lu P31
M
Linlin, Ma K43
Yongxin, Ma K41 K42
Yujie, Ma K39
Yongjun, Mai K38
Yoshihiro, Mano PL4
Fangguan, Mao K36
Lijian, Mao P04
Daniel, Mathieu PL8
Xiufang, Mei P11
Ying, Mei K23
Cristian, Melián P40 P41
Bing, Meng K40
Juan, Meng K28
Jun, Meng K33
N
Yulin, Nie K26
Ko-Chi, Niu PL7
P
Fuqiong, Pan OR15
Hekui, Pan K138
Jiexiang, Pan K29 K31
Lin, Pan K127
Shuyi, Pan OR18 K25
Xiaorong, Pan K136
Xiaowen, Pan P01 P16
Jinjun, Pang K37
Huiping, Peng K63
Ya, Peng K122
Zhaoyun, Peng K65
Zhengrong, Peng OR29
Q
Yan, Qi K178
Chunling, Qian K177
Haiying, Qiu K146
Jun, Qu K27
Cuixia, Que K196
Lei, Que K185 K186
R
Cunge, Ren K176
Ping, Ren K169
S
Jingyi, Shao K175
Paul J., Sheffield B2
Hansheng, Sheng K174
Chunmei, Shi K197
Raymond C, Shields P43
Danhong, Song K147
Juan, Song K173
Li, Sun K172 K209
Lin, Sun K171
Xuejun, Sun PL9
T
Zhongquan, Tang K179
Hengyi, Tao PL3
Jinzhong, Teng K163
Joseph, Ting K87
Chaoqun, Tu K17
V
Johannes, von Reumont
P50
W
Chia-Ti, Wang P39 P48
Gang, Wang PL6 P09
Guohua, Wang P03
Guozhong, Wang K162
Hongjuan, Wang P45
Jun, Wang P17
Liping, Wang P07 K67
Min, Wang K161
Peidong, Wang OR22 OR24
Qinghong, Wang P35
Qiusha, Wang K158 K159 K160
Rongzhen, Wang K134
Shengzhi, Wang K157
Shuzhen, Wang K156
Shyanher, Wang P49
Wei, Wang K155
Wenjie, Wang K72
Wenlan, Wang OR19
Xiaohong, Wang K154
Xiaoli, Wang P23
Yalin, Wang K153
Yong, Wang OR27
Youbin, Wang OR26
Youcun, Wang K152
Yuhua, Wang K164
Chengwen, Wei K195
Fen, Wei K182
Qibiao, Weng OR23 K198
Lianhua, Wu K206 K207
Qiaoning, Wu K194
Ruilan, Wu K202
Shuanggui, Wu K203
Xiuyun, Wu K71
Yali, Wu K204
Yanqiu, Wu K205
Zhide, Wu K192
X
Hongzhi, Xia K208
Haibin, Xiang K139 K193
Pingtian, Xiao P24 K190 K191
Jinxiang, Xie K180
Qiuyou, Xie P02
Rong, Xie K189
Xiaoping, Xie P27
Zhihui, Xie K187
Lili, Xu K01
Limin, Xu
K85
Y
Kazuyoshi, Yagishita OR04
Maoxing, Yan K129
Xiaomin, Yan K184
Chen, Yang P36
Haiping, Yang K151
Jianhua, Yang K183
Jingfu, Yang P05
Jinhua, Yang K94
Lin, Yang K119 K120
Ping, Yang K133
Qunfang, Yang K118
Quyun, Yang K201
Wenping, Yang K117
Xiaolin, Yang K116
Xin, Yang K115
Yuanbi, Yang K128
Yujia, Yang P06 P26
Zheng, Yang K114
Yue, Yao K91
Shifu, Yin K113
Daoyuan, Yu K110
Guoping, Yu K08
Ning, Yu K112
Qiuhong, Yu P22
Ronghao, Yu P29 P46
Xuelai, Yu K126
Youjie, Yu K111
Jing, Yuan K121
Lina, Yue K210
Z
Shaoxia, Zeng K109
Denghua, Zhang K93
Dongyun, Zhang K75
Hechun, Zhang K107
Jian, Zhang K137
Jin, Zhang P37
Li, Zhang K166
Ludi, Zhang K105
Wenli, Zhang K168
Yan, Zhang K103
Yi, Zhang K102
Yubao, Zhang K145
Hui, Zhao K101
Chenggang, Zheng K100
Ming, Zhong K99
Qiaofen, Zhong P32
Dun, Zhou K98
Hongtu, Zhou K95 K96 K97
Kunru, Zhou K135
Shurong, Zhou OR16 K106
Xuming, Zhou K108 K148 K149
Yihong, Zhou K144
Hourong, Zhu K143
Jianming, Zhu K140 K141 K142
Stránka 1: [1] Komentář [L7]
Lenvov
19.10.2008 4:39:00
Lenvov
2.2.2009 3:03:00
正确的称呼？
Stránka 1: [2] Odstraněno
Table of Contents
Welcome Message from Conference President ......................................................................................... 1
Welcome Message from ICHM Executive President................................................................................... 2
Committees ................................................................................................................................................ 2
Conference Information ............................................................................................................................... 3
Scientific Information.................................................................................................................................... 5
Detailed Program (Oct. 27) .......................................................................................................................... 8
Detailed Program (Oct. 28) ........................................................................................................................ 12
Detailed Program (Oct. 29) ........................................................................................................................ 16
大会主席致辞 ............................................................................................................................................. 19
ICHM执行主席致辞
会议组织结构 ............................................................................................................................................. 21
会议基本信息 ............................................................................................................................................. 22
学术信息 .................................................................................................................................................... 23
详细日程 (10月27日) .................................................................................................................................. 25
详细日程 (10月28日) .................................................................................................................................. 28
详细日程 (10月29日) .................................................................................................................................. 31
Poster Program壁报日程 ........................................................................................................................... 33
Acknowledgement 鸣谢 ............................................................................................................................. 48
Exhibitors 展商 ........................................................................................................................................... 50
论文集 Abstracts ........................................................................................................................................ 50
Index 索引.................................................................................................................................................. 56
Konec oddílu (další stránka)
1
Welcome Message
Dear friends and colleagues,
th
Welcome to Beijing for the 16 international Congress on Hyperbaric Medicine (ICHM2008).
The world has witnessed the rapid economic and social development in China in the recent years. The
HBO medicine is developing and advancing together with the economic growth. In particular, it has got
inspiring achievements in China in the basic and clinical research of HBO therapy in the treatment of
delayed neuropsychologic sequelae, ischemic cerebral vascular diseases and high altitude diseases.
Chinese health professionals are willing to share our experience with our colleagues around the world.
The ICHM2008 will be a summit meeting for all leading specialists in the field of h[l1]yperbaric and
u[l2]nderwater Medicine, and a grand gathering for basic researchers and clinical experts from all over the
world. World-renowned experts will give lectures during the congress. I am sure their speeches must be
both educational and interesting.
We also invite your family and friends to come with you. We have arranged special tours for your
accompanying persons. I believe they will enjoy their stays in Beijing when you have busy scientific
schedule at meeting.
A rich cultural and historic heritage, accumulated through the passage of time, adds an extraordinary aura
to Beijing. The five dynasties that chose Beijing as their capital have left abundant antique treasures.
Travelers would marvel at every turn on a tour to this hottest destination of the world today: from the
grandiose Forbidden City to the gorgeous Great Wall, and from the labyrinth-like hutong to other worldly
royal mausoleums.
Wish you a good stay in China.
Prof. Gao Chunjin
Congress President[l3]
16th International Congress on Hyperbaric Medicine
Konec oddílu (další stránka)
2
Welcoming Address to the Participants of the XVI-th Congress of the International Congress on
Hyperbaric Medicine (ICHM).
Exactly 45 years ago, in 1963, the First Congress of the ICHM took place in the Surgical Clinic of the
University Hospital, the Wilhelmina Gasthuis in Amsterdam, the Netherlands. Professor Ite Boerema, the
“father of Hyperbaric Medicine” and professor of Surgery and his Staff had invited many colleagues from
all over the World to discuss the first results of the application of Hyperbaric Oxygen Drenching, as
Boerema used to call it, in a variety of indications.
The most prominent indications at that time were Infectious diseases mainly Anaerobic Infections,
Coronary Infarction, Experimental Cancer Therapy in a combination with Chemotherapy and
Hyperthermia. Also the use of HBO in Carbon Monoxide poisoning, both experimental and clinically was
discussed. Much attention was given to Physiological and Pharmacological problems and Oxygen Toxicity,
with this new mode of therapy. Cardiovascular Surgery in combination with HBO and Hypothermia, the first
and for some time the only indication, was also a leading subject. Both experimental and clinical results
were reported. Building problems of chambers and Safety were also not forgotten. Participation to that
Congress was strictly on invitation by Boerema himself, at that time well thought of, but fortunately the one
and only time that this happened in our history. The participants came from the USA, the UK, France,
Australia and South Africa. In a historical review Jack Jacobson II of New York started with mentioning the
Diving Bell of Aristotle, used in 332 BC by Alexander the Great in his siege of Tyre. So our history of diving
and hyperbaric medicine goes back almost as far as medicine in general.
From that time on regular Congresses were held at a four-yearly (until 1981, Moscow) and later threeyearly interval.
The first time that the Congress moved to Asia was in 1969 when we met in Sapporo, Japan. In 1993 the
Congress gathered in China, in Fuzhou under the chairmanship of the late professor of Cardiac Surgery,
Wen Ren Li.
We are very happy that we are back in China today thanks to the efforts of our present chairman,
professor Gao Chunjin and his whole team of the Chinese Medical Association (mrs. Chenchen) and the
Chinese Hyperbaric Medical Society.
I must say that I have been much impressed in the foregoing two years by the very solid and precise
preparations of the Organizing Committee. Nothing has been left to chance. The same has been noticed
by the whole world in the Organisation of the Olympic Games and the Paralympic Games just before our
Congress this year.
On behalf of the Executive Committee of the ICHM and all participants here, I will extend my sincere
thanks and admiration to prof Gao Chunjin and his staff for this excellent organization.
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We all look forward to the coming days, both professionally and socially. It is always a pleasure to meet old
friends again in these nice surroundings.
There will be also much work to do. The Executive Committee will lose two of its long standing members,
our Secretary-Treasurer Fred Cramer and our Executive Director, Dirk Jan Bakker. After many years of
serving you and because of our ages we will resign during this Congress and we will choose new
members during the Governors Luncheon. I myself will stay involved in matters of the Congress but not
any more in an official position.
From this point I want to thank Fred very sincerely for his never ending efforts to advertise the Congress
on various occasions, to solicit for new Membership and finding sponsoring for the Newsletter, which was
really not an easy task. It will be difficult to replace him.
Well ladies and gentlemen, dear colleagues and friends, I am convinced that we will have an excellent and
rewarding time here in the magnificent historical city of Beijing, both scientifically and socially. I wish you all
a very pleasant and happy stay.
Thank you very much for your attention.
D.J.Bakker, MD PhD
h.t. Executive Director of the ICHM.
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Committees
President: Gao Chunjin, Department of Hyperbaric Medicine, Chaoyang Hospital, Capital Medical University
Vice Presidents:
Pan Xiaowen, Hyperbaric Medicine Center, Navy General Hospital
Tao Hengyi, Department of Diving Medicine, Second Military Medical University
Secretary General: Tao Hengyi, Department of Diving Medicine, Second Military Medical University
Chair of Logistic Committee:
Wang Peisong, Department of Hyperbaric Medicine, Affiliated Hospital to Qingdao University Medical School
Co-Chair of Logistic Committee:
Weng Qibiao, Department of Hyperbaric Medicine, Zhujiang Hospital, South Medical University
Chair of Scientific Committee: Yi Zhi, Shenzhen People’s Hospital, Guangzhou
Co-Chair of Scientific Committee: Wang Gang, Affiliated Hospital, China Medical University, China
Members:
Joseph Ting (Maylasia), Pan Shuyi, Huang Xiang, Hu Huijun, Li Zhuo, Yang Lin, Lu Yan, Zhang Yue, Xu Weigang
konec oddílu
Congress Information
1. Registration & Information Center
Venue: level 2, Beijing International Convention Center
Add: No.8, Beichendong Road, Chaoyang District, Beijing, China
Opening Hours:
Oct.26 (Sunday)
Oct. 27 (Monday)
Oct. 28 (Tuesday)
Oct. 29 (Thurday)
09:00-18:00
8:00-17:00
8:30-17:00
8:30-11:30
On-site Registration fee:
Non Member
￥5,500
Accompanying Person
￥2,000.00
* Member
￥5,000
* *Nurse & Trainee
￥2,500.00
* Member is ICHM live member.
* * A copy of student ID or a verification letter from his or her supervisor are requested.
2. Participant Identification and Entitlements
Please wear your Congress badge during the Conference.
Red
Delegate
Yellow
Accompanying person
Green
Exhibitor
Blue
Staff
Red Ribbon Invited Speaker / Chair / Committee Members
3. Congress Official Language
English
4. Coffee/Tea Break
Coffee, tea and snacks will be provided
Oct. 27
Oct. 28
10:40-11:00am
10:00-10:30am
15:00-15:30pm
15:00-15:30pm
Oct. 29
10:00-10:30am
15:00-15:30pm
5. Meals
Oct. 26
Oct. 27
Oct. 28
Oct. 29
Lunch
N/A
Beijing Continental
Grand Hotel
Beijing Continental
Grand Hotel
Beijing Continental
Grand Hotel
Dinner
Beijing Continental
Grand Hotel
Welcome reception
(Laoshe Theater)
Conference Dinner
5
6. Exhibition
Area:
Exhibit Hours: 09:00-17:00 Oct. 27 and 28, 09:00-12:00 Oct. 29, 2008
7. Accommodation
For any inquiry about the hotel, please go to the Registration/Information Center (Level 2, Beijing
Continental Grand Hotel).
8. Social Program
Oct. 26 Acrobatics with dinner
Price: 300 yuan
The usual reaction to a Chinese acrobatics show is something like “Whoa! That was so…” The contortions
and balancing acts are quite incredible. Audients will be at the edge of their seats through most of the
show saying “don’t fall, don’t drop it!” and incredibly, they never do.
Oct. 27 Welcoming banquet (Roast duck dinner and Peking opera)
Free to all fully-paid participants and accompanying persons. Additional ticket: 340 yuan
Audients will be led to a downtown theatre for Peking Operas, face changing opera and etc. Beijing Roast
duck and other traditional Peking dishes will be served as well.
Oct. 28 Conference Banquet
Free to all participants and accompanying persons. Additional ticket: 200 yuan
Oct. 29 Olympic Venue visits/Hospital visit
The organizing committee invites you to some must-go Olympic venues like Bird's Nest and Water Cube.
Free to all participants.
Oct. 29 The Night of Beijing
Price: 425 yuan
The show is a dinner / theatrical performance unsurpassed in Asia. A luxurious artistic feast will be
presented in this delicately decorated theatre that you can hardly resist. The brilliant Beijing Opera, the
elegant Chinese dance, the magnificent acrobatics and marvelous Chinese Kongfu are well integrated with
the beautiful costumes and settings. The pleasing music and spotlights and other modern stage arts will be
a real shock of beauty and unforgettable experience to you in China. What’s more, the delicious Beijing
food served in the theatre will make your evening more enjoyable.
9. Accompanying person’s tour – Exciting shopping tour
Date: Morning, Oct. 29, 2008
A tour voucher is included in the accompanying person’s registration package. Please bring the voucher to
the Tour Desk of Registration Area at level 2, Beijing International Convention Center to sign your name
for and learn more information about the tour.
10. Post-Congress Inquiry
CMA Meeting Planner, Chinese Medical Association
Add: 42 Dongsi Xidajie, Beijing 100710, China
Tel: +86 10 8515 8148 Fax: +86 10 6512 3754 Email: chenchen@cma.org.cn
konec oddílu
6
Scientific Information
1. Speakers:
Please hand in your MS PowerPoint slides at least one hour before your presentation to the conference
room staff.
2. Session Chairs:
We suggest you meet the staff inside the meeting room in case of any adjustments in the program or
speaker absences.
3. Poster:
Poster area: Exhibit Hall 2, Level 1, Beijing International Convention Center
Language: English
Presentation Time:
Oct. 27
Oct. 28
Oct. 29
10：40 – 11：00
10：00 – 10：30
10：00 – 10：30
15：00 – 15：30
15：00 – 15：30
Your poster will be displayed throughout the entire congress. Please stand by your poster and make
presentation during the above mentioned timeslots. Please hand in your poster to the Poster Desk at the
Registration Area and take off your poster 12:00-12:30, Oct. 29.
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7
Detailed Program
Oct. 27
8:30-9:00 Opening Ceremony
Chairs: Zhi Yi
9:00-10:40 Invited Session 1
Chairs: Xiaowen Pan, Jordi Desola
9:00-9:50
B1 The use of Hyperbaric Oxygen in Necrotising Soft Tissue Infections: A Historical Perspective with
an Update anno 2008
D. J. Bakker, MD, PhD. Amsterdam, the Netherlands
9:50-10:40
B2 Role of Tissue Oximetry in Assessment of Problem Wounds
Paul J. Sheffield, PhD; International ATMO, Inc, Nix Wound Healing Center, San Antonio, Texas, USA
10:40-11:00 Break
11:00-12:00 Invited Session 2
Chairs: Peisong Wang, Ko-Chi Niu
11:00-11:30
PL1 The Indication of Hyperbaric Oxygen Therapy and for Orthopaedics in Japan
Mahito Kawashima, Takashi Yamaguchi, Kawashima Orthopaedic Hospital, Japan
11:30-12:00
PL2
The Use of “Deep Stops” In Preventing Precordial Detectable Doppler Bubbles in
Recreational Scuba Divers
Frans J. Cronjé, MBChB, MSc, DAN Southern Africa;
lunch
13:30-15:00 Invited Session 3
Chairs: Paul J. Sheffield, Qibiao Weng
13:30-14:00
PL3 An Overview of Diving and Diving Medicine in China
Tao Hengyi, Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical
University, Shanghai, China
14:00-14:30
PL4 DCS incidence of commercial dive works in Japan and Evaluation of the profile on multi-level
diving by integration of nitrogen accumulation in each body tissue
Yoshishiro Mano, Hyperbaric Center, Tokyo Medical & Dental University Hospital, Japan
14:30-15:00
PL5 Traumatic Spinal Cord Injuries – is There a Role for HBO?
Jochen Freier, MD, Consultant für Diving and Hyperbaric Medicine (EDTC), Anaesthesiology and
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Intensive Care, Vice President of the German Society für Hyperbaric Medicine (GTUEM), Tagesklinik
Hofheim, Germany
15:30-17:15 Free Paper Session 1
Charis: Pingtian Xiao, Mano Yoshihiro
15:30-15:45
OR01 HBO and cytostatic chenotherapy
Christtian Heiden, German Professional Soc. Hyperb. Chambers
15:45-16:00
OR02 Hyperbaric Oxygen: Does it Promote Cancer Recurrence or Metastasis? An Update
John Feldmeier, University of Toledo Medical Center|Radiation Oncology Department; Toledo
Radiation Oncology
16:00-16:15
OR03 Hyperbaric Oxygen for Complications of Oncological Treatment of Paediatric Patients
Michal Hajek, Centre of Hyperbaric Medicine, Municipal Hospital of Ostrava, Czech Republic
16:15-16:30
OR04 Hyperbaric Oxygen may accelerate tumour-bearing mice to death
Liu Qingle, Changhai Hospital, the Second Military Medical University, Shanghai, China
16:30-16:45
OR05 Hyperbaric oxygenation (HBO) in mediastinitis treated with an open mediastinum after cardiac
surgery
Jacek Kot, National Center for Hyperbaric Medicine, Medical University, Gdansk, Poland
16:45-17:00
OR06 Normobaric and hyperbaric oxygen therapy for migraine and cluster headache
Michael Bennett, Dept. Diving and Hyperbaric Medicine, Prince of Wales Hospital, Sydney, Australia
17:00-17:15
OR07 Hyperbaric oxygen therapy induces antiinflammation and antipyresis in experimental studies
Lin Mao-Tsun, Department of Medical Research and Department of Hyperbaric Oxygen therapy, ChiMei Medical Center, Taiwan, China
17:15-17:30 Satellite symposium
Konec oddílu (další stránka)
9
Oct. 28
8:30-10:00 Invited Session 4
Chairs: Jochen Freier, Qingle Liu
8:30-9:00
PL6 Hyperbaric Oxygen Therapy in Acute Traumatic Peripheral Ischemic Diseases
Wang Gang M.D. Ph.D., Emergency Dept & Hyperbaric Oxygen Dept., First Affiliated Hospital, China
Medical University, Shenyang, China
9:00-9:30
PL7 The Application of Hyperbaric Oxygen Therapy in Critical Disorders
Niu Ko-Chi, Department of Medical Research and Department of Hyperbaric Oxygen, Chi Mei Medical
Center, Tainan, Taiwan, China
9:30-10:00
PL8 Hyperbaric Medicine: an European Perspective
Daniel Mathieu, Centre Hyperbare, Hôpital Calmette, Centre hospitalier Régional et Universitaire, Lille,
France
10:00-10:30 Break/Poster
10:30-12:00 Free Paper Session 2
Chairs: Joseph Ting, Shurong Zhou
10:30-10:45
OR08 Measurement of plasma nitrotyrosine and nitrite after a single treatment with hyperbaric
oxygen in healthy human subjects
RENU AGNIHOTRI, SABA UNIVERSITY SCHOOL OF MEDICINE
10:45-11:00
OR09 HBO in Tokyo Medical and Dental University in 2007 and The Effects of HBO on Soft
Tissue Injury in Sports Activity
Kazuyoshi Yagishita, Hyperbaric Medical Center/ Orthopaedic Surgery, Tokyo Medical and Dental
University
11:00-11:15
OR10
adjunctive use of hyperbaric oxigenacion (HBO2) in the managment of cerebral
hemorrhage in a pregnant patient: a case report
Alberto Chavez, Hospital Angeles Metropolitano
11:15-11:30
OR11 Necropsic Findings Of Syndrome Of Intratoracic Hyperpressure In A Young Diver And A
Decompression Accident After A Deep Scuba Diving In Veteran Diver (Two Particular Cases)
John M Batle, MEDISUB Hyperbaric & Underwater Research Institute Palma de Mallorca SPAIN
11:30-11:45
OR12 24 cases of neurological decompression illness - 14 months of a single center experience
10
Jorge Calderón, hospital Ancud|Pontificia Universidad Católica de Chile
11:45-12:00
OR13
Effect of Hyperbaric Oxygen Treatment on Traumatic Brain Injury Rat by Magnetic
Resonance Imaging
Huang Xiang, Bejing Shunyi Hospital of China Medical University, Beijing, China
13:30-15:00 Free Paper Session 3
Chairs: Frans Cronje, Fuqiong Pan
13:30-13:45
OR14 The effect of hyperbaric oxygenation on diabetic foot ulcers (the HODFU-study). A single
centre, prospective, randomised, double-blind placebo-controlled parallel-group study
Christer Hammarlund, Helsingborg Hospital, Sweden|Lund University Hospital, Sweden|Malmoe
University Hospital, Sweden
13:45-14:00
OR15
First-aid of 12 Gas Gangrene Cases Caused by the Earthquake Injuries by Use of
Hyperbaric Oxygen
Pan Fuqiong, Sichuan Provincial Hospital, Chengdu, China
14:00-14:15
OR16 A survey on HBO treatment of traumatic brain injury this century in China—A important
evidence of HBO improve curative effect and prognosis of Brain injury
Zhou Shurong, Zhou Shurong
First Affiliated Hospital of Nanjing Medical University, Nanjing, China
14:15-14:30
OR17 Clinical analyses of 429 Cases of Acute CO Poisoning
Li Zhuo, Beijing Chaoyang Hospital, Beijing, China
14:30-14:45
OR18 Review of Current situation on acute carbon monoxide poisoning in china
Pan Shuyi, Navy General Hospital, Beijing, China
14:45-15:00
OR19 Effect of HBO on endogenous neural stem cells in rat models of acute CO poisoning
Wang Wenlan, Department of hyperbaric oxygen treatment center, School of Aerospace Medicine,
Fourth Military Medical University, Xi’ an, China
15:00-15:30 Break
15:30-17:15 Free Paper Session 3
Chairs: Daniel Mathieu, JIndong Lu
15:30-15:45
OR20 Hyperbaric oxygen treatment attenuated the decrease in regional glucose metabolism of
rats subjected to focal cerebral ischemia: a high resolution positron emission tomography
11
study
M. LOU, Department of Neurology, the Second Affiliated Hospital, Zhejiang University, School of
Medicine, Hangzhou, China
15:45-16:00
OR21 Treatment of micro-found drilling cranium and hyperbaric oxygen on 94 cases of patients
with extradural hematoma
Cheng Jincheng, 123th Hospital of People’s Liberation Army
16:00-16:15
OR22 The effect of hyperbaric oxygen on the osteoporosis rats
Wang Peisong, Department of hyperbaric oxygenation, The affiliated Hospital of Qingdao University
Medical College, Qingdao, China
16:15-16:30
OR23 Trial of cabin-oxygen-concentration distribution of single oxygen pressurized module
Weng Qibiao, Zhujiang Hospital attached to Southern Medical University, Department of Hyperbaric
oxygen, Guangzhou, China
16:30-16:45
OR24 Clinical Research on Effect of HBO plus Electric Stimulation for Treatment the Cerebral
Resuscitation
Wang Peidong, Chinese HBO Medicine Association Research Center of Brain Recovery, Nanjing,
China
16:45-17:00
OR25 Effect of Hyperbaric Oxygen Treatment on the content of VEGF, HIF-1,CX43 in skin flaps
of rabbits
Liu Xuehua, Department of Hyperbaric Oxygen, Chaoyang Hospital, Capital Medical University
17:00-17:15
OR26 Hyperbaric Oxygenation Preconditioning Induce Skin Flap Ischaemia Tolerance in Rat
Model
Wang Youbin, Plastic Surgery Department of Peking Union Medical College Hospital, Beijing, China
Konec oddílu (další stránka)
12
Oct.29
8:30-10:00 Invited Session 5
Chairs: Christtian Heiden, Qiang Wang
8:30-9:00
PL9 The Pretective Effect of Hyperbaric Oxygen Preconditioning on ischemic/hyoxia Injury
Xuejun Sun, Department of diving medicine, Second Military Medical University. Shanghai, China
9:00-9:30
PL10 Disruption of Viral Pathogenesis Following Patient Exposure to Hyperbaric Environments
with Altered Inspired Gas Mixtures
Stephen D. Guthrie, MD, PhD, Designed Altobaric Research Institute, Livonia, Michigan, USA
9:30-10:00
PL11 Carbon Monoxide Poisoning Clinical Findings, Common Errors, And Early diagnose: A
prospective analysis of 2900 cases
Jordi Desola, MD, PhD, Hyperbaric Therapy Unit of Barcelona, Barcelona, Spain
10:00-10:30 Break/Poster
10:30-11:30 Free Paper Session 5
Chairs: Yunping Ji
10:30-10:45
OR27
The Effect of Hyperbaric Oxygen on Histopathology and Coagulation-fibrinolysis
Systems in Rabbits with Steroid-induced Avascular Necrosis of the Femoral Head
Wang Yong, Department of Hyperbaric oxygen, Fuxing Hospital, Capital Medical University, Beijing,
China
10:45-11:00
OR28
Hyperbaric oxygen preconditioning protects against traumatic brain injury at high
altitude
Hu Shengli, Department of Neurosurgery, Southwest Hospital of the Third Military Medical University,
Chongqing, China
11:00-11:15
OR29
The effect of hyperbaric oxygen on the proliferation and death of Nasopharyngeal
Carcinoma Cells and its mechanisms
Peng Zhengrong, Xiangya Hospital, Central South University, Changsha, China
11:15-11:30
OR30 Effect of Hyperbaric Oxygenation (HBO) on Unilateral Spatial Neglect ( USN)
Liu Jinglong, Heilongjiang Rehabilitation Hospital, Ha’erbin, China
11:30-12:00 Closing Ceremony
Chairs: Zhongquan Tang
Closing Remark, Hengyi Tao[L4]
Konec oddílu (další stránka)
13
大会主席致辞
尊敬的各位同道、各位朋友：
首先，我谨代表大会组织委员会欢迎各位同仁前来北京参加第十六届国际高气压医学学术会议暨第十七届全国
高压氧医学学术会议。
本次会议是国际高气压医学会自1993年以来的[l5]第二次在中国举行会议[l6]，是我国高压氧医学蓬勃发展、加
强国际交流的结果。
近几年，世界见证了中国经济和社会的高速发展，高压氧医学也伴随经济进步的脉搏向前超越。特别是应用高
压氧治疗一氧化碳中毒迟发性脑病，缺血性脑血管疾病及高原疾病领域，中国在基础和临床研究方面取得了令人鼓
舞的成绩。中国的医务工作者期盼与世界分享我们的经验，同时也向世界各国的同道学习、交流。
本届大会将是高压氧医学和潜水医学权威学者的一次峰会，也将成为各国研究者及临床专家的盛会。多为（位
）享有盛誉的学者欣然接受邀请到会并作精彩生动的报告。
金秋十月，阳光和煦，微风送爽。继2008年北京奥运会和残奥会之后，古都北京到处充满了新的生机和活力。
我们特地选择了北京国际会议中心作为大会会场，它毗邻奥运村，与“鸟巢”咫尺相隔。会后，感兴趣的代表可参观
奥运赛场。
再次感谢您的光临，并希望各位在北京参会期间愉快、有所收货（获）。
高春锦
第十六届国际高气压医学学术会议主席
中华医学会高压氧医学分会主任委员
Konec stránky
国际高气压医学会主席致辞
国际高气压医学会主席致辞
45年前，即 1963年，第一届国际高气压医学学术会议于
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45年前也就是1963年在
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荷兰阿姆斯特丹Wilhelmina
Gasthuis大学医院外科部隆重召开。受“高压氧医学之父”、
外科教授Ite
Boerema和他的同事之邀，
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各国同仁积极参会并共同探讨
14
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3:03:00
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了最初被Ite Boerema教授称之为“高压
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在不同病种中的应用及疗效
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用于不同症状的初步效果
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，“高压纯氧浸透法”是Ite Boerema教授最初对高压氧疗法的称呼。
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当时高压氧疗法最主要的
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适应症为以厌氧菌感染为主的感染性疾病，冠状动脉梗塞，结合化
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和高温疗法治疗癌症。大会讨论了
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各种传染病，例如厌氧菌感染，冠状动脉梗塞及试验性癌症疗法。也对
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应用高压氧治疗一氧化碳中毒的临床和基础研究，
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，科研和临床（都）予以了讨论。
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同时，大家还也很关注
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生理、药理以及氧中毒
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问题予以了特别关注。大会的首要议题还包括探讨高压氧结合
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3:03:00
低温疗法辅助进行心血管手术的临床与基础研究，这在一段时期内曾经是高压氧唯一的也是最主要的适应症，
除此之外，是第一个用途，在一段时间也是惟一的用途，并且是惟一的主题。临床和试验的结果都有所报道。建设
氧仓和安全问题也没有忘记（被忽略）。
那时参会者都是收到了Boerema教授本人的要求（邀请），（那时）是经过深思熟虑的，但是幸运的是，这是
惟一一次也是最后一次（在我们的历史上是空前绝后的）。参会者来自美国，英国，法国，澳大利亚和南非。在历
史回顾中，来自纽约的Jack
Jacobson
II首先提到了公元前332年亚历山大大帝在提尔城之围中用到了亚里士多德的潜水钟。如此看来，潜水和高气压的历
史与医学史一样古老。
从那时起每四年召开一次会议，直到1981年莫斯科会议以后，变为每三年举办一次。国际高压氧医学会首次亚
洲会议是于1969年在日本札幌举行。1993年在中国心脏外科专家李温仁教授的主持下，我们首度在中国福州举办
15
了国际高压氧医学学术会议。我们非常高兴今天再次来到中国举办会议，感谢今天的大会主席高春锦教授，中华医
学会及中华医学会高压氧医学分会所做的努力。
在刚刚结束的奥运会和残奥会中，我们看到了优秀的组织工作。而在过去的两年中本次会议组委会所作的具体
而细致的准备工作同样让我印象深刻。
我代表国际高压氧医学会执行委员会和各位参会者，向高春锦教授和她的团队表示衷心的感谢。未来的几天里，
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我们无论从学术上还是活动上都期待接下来的几
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全体代表将共享这次盛会
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能在这样美好的
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。
未来的高气压医学任重而道远，由于年龄原因，在为各位服务多年后，我们的财务秘书[L7]
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Fred Cramer和执行主席Dirk Jan Bakker将要退出执行委员会。
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由于年龄原因，在为各位服务多年后，我们将要退出，在委员午餐中选出
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新的成员将在委员会会议中选举产生。我本人
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HBO in Tokyo Medical and Dental University in 2007 and The Effects of HBO on Soft Tissue Injury in
Sports Activity
Kazuyoshi Yagishita, Nobuo Yamami, Seiichiro Togawa, Yoshihiro Mano
Hyperbaric Medical Center, Tokyo Medical and Dental University, Tokyo, Japan
Objective: In our university hospital, multiplace chamber including 3 rooms and the capacity of 16 patients was
set up in 2001. In 2007, 7970 times hyperbaric oxygen therapy (HBO) in 958 patients were performed, which is
the most patients number in one institute in a year in Japan. The purposes of this study were to report the
diseases and the patients performed with HBO in our hospital last year. The effects of HBO on crush injury and
compartment syndrome were well documented by many authors, and randomized controlled studies revealed the
effects of HBO on reduction of necrosis and edema. We now perform HBO aggressively to soft tissue injury
including compartment syndrome, ankle sprain, knee ligament injury, and muscle strain. The purposes of this
study were also to investigate the effect of HBO on soft tissue injury in sports activity. Patients and Methods:
7970 times HBO in 958 patients were performed in our hospital in 2007. The number of the patients and HBO
times were evaluated. In 180 patients with soft tissue injury in sports activity, visual analog scale (VAS) scores
and recovery time to previous sports activity were evaluated. Results: The number of the patients and HBO times
in decompression illness were respectively 374 patients and 764 times, 87 patients and 934 times in sudden
deafness, 34 patients and 740 times in peripheral vascular disorder including diabetes and arteriosclerosis
obliterans, 47 patients and 1046 times in osteomyelitis, 180 patients and 865 times in soft tissue injury related
sports activity, 90 patients and 1644 times in myelopathy and radicuropathy, 19 patients and 220 times in carbon
monoxide poisoning, and 18 patients and 259 times in radiation-induced cystitis. In patients with soft tissue injury
nd
in sports activity, VAS scores improved compared between pre and post 2-hour HBO. In patients with 2 grade
medial collateral ligament injury of the knee in HBO group, recovery acceleration to previous sport activity was
observed. Conclusion: In HBO procedure in our university in 2007, no major patient troubles and no chamber
problems were recorded. The effects of HBO on soft tissue injury were strongly suggested in this study.
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OR09
Hyperbaric Oxygen may accelerate tumour-bearing mice to death
Qingle LIU, Chenggang Zheng, Xiaohua Hang
Changhai Hospital, the Second Military Medical University, Shanghai, China
Objective: To explore the influence of hyperbaric oxygenation (HBO) on life-span in tumour-bearing
mice. Method: Male Balb/c mice were randomly divided into celiac-tumour group: inoculating tumour cell into
abdominal cavity of the animal, celiac-tumour-HBO group: inoculating tumour cell into abdominal cavity and then
exposing to hyperbaric oxygen, back-tumour group: inoculating tumour cell under skin of the back of the animal,
back-tumour-HBO group: inoculating tumour cell under skin of the back of the animal and then exposing to
hyperbaric oxygen. There are 7 mice in each group. After s-180 tumour cell was translated to mice HBO was
given once a day in concerned group. The life-span and skin ulcer were observed. Results: The life-span is
27.57士4.4693 days in celiac-tumour-group, 24士2.9439 days in celiac-tumour-HBO group, 63.43士21.844 days
in back-tumour group, and 35.14士8.934 days in back-tumour-HBO group. The life-spans in celiac-tumour group
and in celiac-tumour-HBO group are different, but P value is 0.133204; the life-spans in back-tumour group and in
back-tumour-HBO group are significant and P value, 0.009002. Conclusion: The life-span of mice in backtumour-HBO group was shorten by exposing HBO, and life-span in celiac-tumour-HBO group is tendency to be
shorten by HBO exposure. The ulcer formation is postponed by HBO in back-tumour-HBO group.
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The medical oxygen pressurized cabin which is convenient and suitable for patients with different diseases and
ages has been widely used for decades. Meanwhile, it is accepted by a majority of hospitals. To select
appropriate input/output of oxygen, structure and washing method is beneficial to oxygen concentration elevation
and distribution in cabin, because pure oxygen is used as medium for compression. Three ways of oxygen
input/output(on the top, middle and bottom of the cabin), three states of the cabin(with a bed, with a bed and a
patient, without a bed and a patient) and three ways of washing method(continued washing method with boosting
pressure, washing method of boosting pressure of the gap of the door, boosting pressure directly without cabinwashing) was adopted in our trial. To detect oxygen distribution during the procedure of elevated, stable and
decreased pressure, nine sampling outlets which located on the top, middle and bottom of cabin were set.
Delivery pressure in cabin is 0.1 MPa in the meter. Experimental session lasted for 80 minutes. Process of
experiment accorded to the rule of Chinese Medical Association—HBO branch. Measurement range of digital
oxygen analyzer is 0-100%. Interval time of sampling is 2 minutes. Influential factor of oxygen distribution in
cabin-Our research suggests that main influential factor of oxygen distribution are broadside stretcher and
input/output location of oxygen at the bottom of cabin. Oxygen distribution of the structure that broadside stretcher
combined with input/output oxygen at the bottom of cabin is as follow: 90% under the broadside stretcher, 40% on
top of it, while 60% in the middle of it. Such distribution of oxygen is not good for safety of cabin and could not
meet the effect of hyperbaric oxygen therapy. The main reason of asymmetrical distribution of oxygen is that
broadside stretcher exerts negative influence on oxygen diffusion and short-circuit at the bottom of the broadside
stretcher (mal-design of input/output location of oxygen leads to automatic washing beneath the broadside
stretcher). Whereas, the structure mentioned above is the most widely used one in the single pure oxygen
chamber. If the entrance of oxygen is designed on top of or in the middle side of cabin ( on top of the broadside
stretcher) may make oxygen concentration achieve up to 65~75% when the pressure is stable at 0.1MPa, thus,
oxygen distribution will be symmetrical in cabin. Output of oxygen has nothing to do with oxygen concentration in
cabin. However, broadside stretcher not only plays an important role in oxygen diffusion in cabin, but also divides
the cabin into two parts. Therefore, to minimize the broadside stretcher plays a positive role in oxygen diffusion in
cabin. Best way of washing the cabin- Oxygen concentration and distribution in cabin is the same with ordinary
compression procedure that five minutes continued washing method or pressure achieved at 0.1 MPa directly.(
with the cabin washed, oxygen concentration is higher by 2% on top of it, while the middle and the bottom part are
higher by 4% versus not to wash the cabin.) However, washing method of normal pressure of the gap of the door
is the most ideal way because it may elevate oxygen concentration up to 80% in 5 minutes inside the cabin.
Selection of sampling outlet of oxygen-Sampling outlet of oxygen is located on top of the broadside stretcher
in the middle of cabin at present. Oxygen concentration is affected by the following factors: differences of the
width and height of broadside stretcher; irrationality of oxygen input/output design; different ways of washing.
Hence, ordinary method for oxygen detection fails to reflect oxygen distribution in cabin. Solution for symmetric
oxygen concentration is to change oxygen input location and adopt the most ideal way of washing. The specific
way is to have the sampling outlet located on top of the broadside stretcher. Oxygen diffusion in cabin-In our 15
times experiment, we found no alteration of oxygen concentration all over the cabin with the pressure up to 0.1
MPa for 40 minutes. It is suggested that oxygen diffusion didn’t change apparently in both quantity and quality
with the pressure up to 0.1 MPa for 40 minutes. Nevertheless, oxygen diffused obviously when pressure
decreased. Oxygen concentration on the top and the middle of the cabin was almost the same whereas relatively
higher at the bottom of cabin when there existed a broadside stretcher with the pressure of 0.06 MPa inside the
cabin. Oxygen concentration is almost the same in the upper, middle and lower location when the pressure is
about 0.03 MPa.
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