1 ARNR 421 ASSISTED REPRODUCTIVE TECHNOLOGIES SYLLABUS Fall Semester Credits: Sec. 01: Sec. 02: Sec. 03: 4 Lecture Laboratory Laboratory MW 9:00 to 9:50 a.m. MW 1:15 to 2:50 p.m. MW 3:15 to 4:50 p.m. Room 136 Animal Bioscience Bldg. (ABB) AI Facility at the BARTF AI Facility at the BARFT FINAL LAB EXAM: FINAL EXAM: Monday, December 3rd at AI Facility at the BARFT Thursday, December 12th @ 6 – 7:50 am, 136 ABB Required Text: None Instructor: Dr. James G. Berardinelli Professor Office: 307 Animal Bioscience Lab: 334 Animal Bioscience Telephone: 994-5574 (office) or 5566 (lab) Office hours: 10:00 to 11:00 am MTWRF e-mail: jgb@montana.edu Web site: D2L Assisted Reprod Tech w/ Lab - ANSC_421_001_201370 COURSE OBJECTIVES: 1. The student will have a working knowledge of the anatomy of the reproductive tract, and physiology and endocrinology of the estrous cycle and pregnancy of domestic females. 2. The student will have a basic understanding of the current techniques used to synchronize estrus in female cattle and of the management required to implement these technologies. 3. The student will have a general working knowledge of embryonic development and understand the importance fetal membrane fluid and fetal structure as it relates to pregnancy diagnosis in domestic cattle. 4. The student will have a working knowledge of the techniques involved in rectal palpation and ultrasonography of the reproductive tract of the female bovine and understand the basis for emerging technologies used in pregnancy diagnosis in domestic animals, i.e., pregnancy specific protein assays. 5. The student will have a working knowledge of the techniques employed for superovulation and embryo transfer in domestic ruminants. 2 6. The student will be able to distinguish, at least 90% of the time, the difference between pregnant and non-pregnant female cattle by rectal palpation and ultrasonography. 7. The student will be able to detect and discuss the differences of an early, mid, and late pregnant female by using fetal size and fetal fluid volume, and size of placentomes. 8. The student will have a working knowledge of the techniques required for artificial insemination of female farm animal species and specific knowledge of those techniques for insemination of female cattle. 9. The student will be able to handle and manipulate frozen semen for optimizing artificial insemination in female cattle. 10. The student will be able to successfully inseminate a minimum of 50 cows during the course and be exposed to the technique employed to successfully transfer embryos in female cattle. METHODS: 1. Lectures and discussion. 2. Handouts, diagrams, and videotapes. 3. Laboratory materials (hands-on materials) a) b) c) d) reproductive tract (pregnant and non-pregnant) tools of rectal palpation and other technical devices, i.e., ultrasound exposure to materials and methodologies for embryo transfer pregnant and non-pregnant cows 4. Handling and manipulating reproductive tracts of pregnant and non-pregnant cattle in situ. 5. Rectal palpation of female cattle at different stages of pregnancy. 6. Evaluating temporal changes in individual cows to demonstrate how the tactile properties of the uterus change as pregnancy progresses (if at all possible). 7. Written and practical examinations to be given twice during the semester to assess student progress and allow instructor to evaluate methods of instruction. 3 STUDENT EVALUATION (GRADING): I. Grades will be assigned by Drs. James G. Berardinelli and Michael Wehrman. Fifty percent of the grades in this course will be assigned by each instructor derived from the following evaluations: A. B. 50% of Pregnancy Diagnosis: 1. Written mid-term exam (20%) 2. Laboratory quizzes that include manual and ultrasonic determination of pregnancy (80%) 50% of Artificial Insemination: 1. AI (50%) a. Grade is calculated based on (Total number of cows successfully passed / 50) with individuals passing 50 or more getting full credit. (45 passed/50 = 90% or 45 points) 2. Semen handling test (30%) a. You get three times to pass the test with a score greater than 25 points (83.3%). i. First test is worth full points (max final score 30 points, 100.0%) ii. Second test you loss 2 points (max final score 28 points, 93.3%) iii. Third test you loss 4 points (max final score 26 points, 86.7%) b. Each RED Unsatisfactory mark is automatic disqualification (major error). c. Each BLACK unsatisfactory mark is a loss of 1 point. d. You can take the test at the beef center during lab or in the reproduction lab at Linfield Hall with prior arrangement with Dr. Wehrman or the Graduate student TA’s. e. Test must be completed between Wednesday Nov 28th to Friday Dec 7th 5:00 PM f. You must have 15-20 minutes to review mistakes before you can retake the test. 3. Written final exam (20%). 4 C. Attendance. 1. D. II. Will count as 10% of grade a. attendance will be taken for each lab period. b. each lab will count as 10 points; attending all labs will yield 100% of the points for the 10% of the attendance grade. Willingness to participate. 1. Subjective in nature and will be given at discretion of instructor. 2. Designated as either a "+" or "-". 3. Based upon an individual’s attempt to participate in discussion, ask pertinent questions, works cooperatively with other students, and attempts to learn more about the advantages and disadvantages of the techniques used in class. 4. This evaluation will not count in your final grade; however, it will be placed in our class record book so that I will be able to refer to when writing letters of recommendation or answering questions concerning the abilities of students to perform in a particular job by prospective employers. Grades will be based upon the lettering system, A, B, C and F. A= 90 to 100% B= 80 to 89% C= 70 to 70% F= less than 70% Note: there will be no "D" grades assigned, if you cannot do "C" work you cannot palpate or inseminate cows!!! EXTRA NOTE: Failure to take the final practicum in Pregnancy Diagnosis portion of the course or failure to take the written final for Artificial Insemination will result in a letter grade of F; NO EXCEPTIONS 5 TEACHING ASSISTANTS Swartz, Jeffrey D. Graduate Student GTA 1 – 5 PM MW Perz, Kate Graduate Student GTA 1 – 5 PM MW Butler, Molly R. Graduate Student 3 – 5 PM MW Schuett, Blade W. Undergraduate Student Quinlan, Russell E. Undergraduate Student Haugland, Tycer L. Undergraduate Student Ehlke, Jane'a M. Undergraduate Student GTA Lab Assistant Lab Assistant Lab Assistant Lab Assistant 1 – 5 PM MW 1 – 5 PM MW 1 – 5 PM MW 1 – 5 PM MW 6 The following MSU policies will be enforced in this class: Behavioral Expectations Montana State University expects all students to conduct themselves as honest, responsible and lawabiding members of the academic community and to respect the rights of other students, members of the faculty and staff and the public to use, enjoy and participate in the University programs and facilities. For additional information reference see http://www2.montana.edu/policy/student_conduct/student_conductc-code_2006-2007.htm. Collaboration University policy states that, unless otherwise specified, students may not collaborate on graded material. Any exceptions to this policy will be stated explicitly for individual assignments. If you have any questions about the limits of collaboration, you are expected to ask for clarification. Plagiarism Paraphrasing or quoting another’s work without citing the source is a form of academic misconduct. Even inadvertent or unintentional misuse or appropriation of another's work (such as relying heavily on source material that is not expressly acknowledged) is considered plagiarism. If you have any questions about using and citing sources, you are expected to ask for clarification. Academic Misconduct Section 420 of the Student Conduct Code describes academic misconduct as including but not limited to plagiarism, cheating, multiple submissions, or facilitating others’ misconduct. Possible sanctions for academic misconduct range from an oral reprimand to expulsion from the university. Section 430 of the Student Code allows the instructor to impose the following sanctions for academic misconduct: oral reprimand; written reprimand; an assignment to repeat the work or an alternate assignment; a lower or failing grade on the particular assignment or test; or a lower grade or failing grade in the course. More serious sanctions require a Conduct Board hearing. You may wish to make explicit the minimum sanction you will impose for willful acts of academic misconduct. Academic Expectations Section 310.00 in the MSU Conduct Guidelines states that students must: A. B. C. D. E. be prompt and regular in attending classes; be well prepared for classes; submit required assignments in a timely manner; take exams when scheduled; act in a respectful manner toward other students and the instructor and in a way that does not detract from the learning experience; and F. make and keep appointments when necessary to meet with the instructor. In addition to the above items, students are expected to meet any additional course and behavioral standards as defined by the instructor. Withdrawal Deadlines After the third exam, I will not support requests to withdraw from this course with a “W” grade unless extraordinary personal circumstances exist. 7 Students with Disabilities If you have a documented disability for which you are or may be requesting an accommodation(s), you are encouraged to contact your instructor and Disabled Student Services as soon as possible. Email Policy I expect that you will check your university email at least every [not required for this class] days From Students email policy: Faculty will determine how electronic forms of communication (e.g., email, MyMSU course announcements, chats and message boards) will be used in their classes, and will specify their requirements in the course syllabus. This official student electronic communications policy will ensure that all students are able to comply with electronic-based course requirements specified by faculty. Faculty can therefore make the assumption that students' official email and MyMSU accounts are being accessed and they can use email, MyMSU course announcements, chats and message boards for their classes accordingly. Student Educational Records All records related to this course are confidential and will not be shared with anyone, including parents, without a signed, written release. If you wish to have information from your records shared with others, you must provide written request/authorization to the office/department. Before giving such authorization, you should understand the purpose of the release and to whom and for how long the information is authorized for release. Students have the right to access their educational records by appointment. This information is protected by the Family Educational Rights and Privacy Act (FERPA). For more information contact the Dean of Students office at 994-2826.