1. health and fitness
2. disease

FEMS Microbiology Reviews Advance Access published April 22, 2015
FEMS Microbiology Reviews, fuv015
doi: 10.1093/femsre/fuv015
Review Article
REVIEW ARTICLE
Giving structure to the biofilm matrix: an overview
of individual strategies and emerging common themes
Laura Hobley1 , Catriona Harkins1 , Cait E. MacPhee2
and Nicola R. Stanley-Wall1,∗
1
Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH,
UK and 2 James Clerk Maxwell Building, School of Physics, University of Edinburgh, Edinburgh EH9 3JZ, UK
∗ Corresponding author: Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK. Tel: +44(0)1382 386335;
E-mail: n.r.stanleywall@dundee.ac.uk
One sentence summary: Examining the structure and function of the biofilm extracellular matrix.
Editor: Sonja-Verena Albers
ABSTRACT
Biofilms are communities of microbial cells that underpin diverse processes including sewage bioremediation, plant growth
promotion, chronic infections and industrial biofouling. The cells resident in the biofilm are encased within a self-produced
exopolymeric matrix that commonly comprises lipids, proteins that frequently exhibit amyloid-like properties, eDNA and
exopolysaccharides. This matrix fulfils a variety of functions for the community, from providing structural rigidity and
protection from the external environment to controlling gene regulation and nutrient adsorption. Critical to the
development of novel strategies to control biofilm infections, or the capability to capitalize on the power of biofilm
formation for industrial and biotechnological uses, is an in-depth knowledge of the biofilm matrix. This is with respect to
the structure of the individual components, the nature of the interactions between the molecules and the
three-dimensional spatial organization. We highlight recent advances in the understanding of the structural and functional
role that carbohydrates and proteins play within the biofilm matrix to provide three-dimensional architectural integrity and
functionality to the biofilm community. We highlight, where relevant, experimental techniques that are allowing the
boundaries of our understanding of the biofilm matrix to be extended using Escherichia coli, Staphylococcus aureus, Vibrio
cholerae, and Bacillus subtilis as exemplars.
Keywords: amyloid fibres; biophysics; biofilm matrix assembly; hydrophobin; Bacillus subtilis; Escherichia coli; Vibrio cholerae;
Staphylococcus aureus
INTRODUCTION
A behaviour that connects microorganisms living in diverse
environments is the formation of sessile social communities
(Costerton et al. 1987). The word ‘biofilm’ was coined to describe
these assemblages that are now widely regarded as the major
reservoirs of bacteria and other microbes in the environment
(Costerton et al. 1987). Biofilms have been identified as playing a role in beneficial interactions, including symbioses with
other organisms, such as the formation of biofilms on plant
tissues and the colonization of the squid by the luminescent
bacteria Aliivibrio fischeri (formerly Vibrio fischeri) (Nyholm et al.
2000; Bais, Fall and Vivanco 2004; Yaron and Romling 2014). Conversely, biofilms have been implicated as being involved in many
different chronic bacterial infections. For instance, the bacterial consortium that infects the lungs of people with the genetic disease cystic fibrosis is now recognized as being within a
highly mucoid biofilm matrix, making them less susceptible to
Received: 18 December 2014; Accepted: 9 March 2015
C FEMS 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium,
provided the original work is properly cited.
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FEMS Microbiology Reviews
antibiotic treatment (Sherrard, Tunney and Elborn 2014). Moreover, the bacteria that cause infections in indwelling medical devices, such as catheters and artificial joint implants, are
known to form biofilms during colonization of these devices
(Hall, McGillicuddy and Kaplan 2014).
Biofilms are hallmarked by the production of an extracellular polymeric biofilm matrix (Costerton et al. 1987). This is not a
recent finding as the fact that bacteria are capable of producing
an extracellular material that aids attachment was recognized
in the pre-molecular era by Claude Zobell and Esther Allen, who
reported ‘The film of bacteria may promote the attachment of
macroscopic organisms in different ways. They may form a mucilaginous surface to which the fouling organisms in the planktonic or free-swimming stage readily adhere until they can prepare their own holdfast’ (Zobell and Allen 1935). It is now known
that the biofilm matrix produced by the majority of organisms
commonly comprises eDNA, lipids, exopolysaccharides and extracellular proteins, many of which exhibit amyloid-like properties (Branda et al. 2005; Flemming and Wingender 2010). It is the
production of the biofilm matrix that underpins the remarkable
success of biofilm communities in allowing the propagation and
survival of cells in their local environment (Davey and O’Toole
2000).
The function of the extracellular matrix within the biofilm
is diverse and consistent with this it has a variable composition across diverse microbial species (Flemming and Wingender
2010). One strategy adopted by several species of bacteria to impart structural integrity/rigidity to the biofilm is the synthesis of
protein fibres that form a scaffold onto which the cells and other
matrix components (such as exopolysaccharides) are attached
(Barnhart and Chapman 2006; Branda et al. 2006; Borlee et al.
2010). Other components in the matrix fulfil a protective function for the inhabitants. For instance, the bacterial hydrophobin
BslA forms a water-resistant ‘raincoat’ over the Bacillus subtilis
biofilm, and the cellulose produced by Escherichia coli biofilms increases the resistance of the community to desiccation (Gualdi
et al. 2008; Kobayashi and Iwano 2012; Hobley et al. 2013). Further matrix components facilitate interactions between bacteria and host cells: for example, while curli fibres produced by
E. coli form a structural component of the biofilm (Chapman et al.
2002; Serra et al. 2013b), they are also required for the attachment
of the E. coli cells to a variety of protein components of the host
cells at the onset of infection (Olsen, Jonsson and Normark 1989;
Sjobring, Pohl and Olsen 1994; Ben Nasr et al. 1996). Detailed understanding of the molecular function of such components is
critical to the ability to control initiation, stabilization or dispersal of biofilms.
Here we will discuss the molecular function of the exopolysaccharides, extracellular proteins and appendages (such
as cell-wall-anchored proteins and flagella) that form the biofilm
matrix. We focus on four bacterial species: E. coli, B. subtilis,
Staphylococcus aureus and Vibrio cholerae, as the biofilm matrix
composition and structure has been extensively studied for
these organisms. We will examine the similarities between the
underlying mechanisms deployed by these bacteria in building
a structured community. At the same time, we highlight some
of the different, and possibly unique, mechanisms that have
evolved. We will also detail how recent advances in technology, in particular microscopy and spectroscopy tools, have allowed a more comprehensive analysis of the in situ components
of the biofilm. Although comparatively new, the field of biofilm
research is at an exciting point, as we move forward from the
concept of biofilms simply consisting of a group of cells in an
extracellular ‘slime’ to the understanding that the matrix is, in
fact, a highly ordered structure that fulfils a great many roles for
the bacteria resident within.
ESCHERICHIA COLI
Escherichia coli is a Gram-negative bacterium in the family Enterobacteriaceae. Whilst it is commonly used as ‘the’ model
bacterium in many laboratory studies, it is a commensal bacterium that resides in the gastrointestinal tract but has the potential to act as an opportunistic pathogen. It can cause a variety
of infections in humans including diarrhoeal disease, urinary
tract infections and sepsis/meningitis (for full reviews on E. coli
pathogenesis, see Kaper, Nataro and Mobley 2004; Clements et al.
2012). Biofilm formation by E. coli has been extensively studied
at the molecular level over the past 20 years. It can form biofilms
on a variety of surfaces, including submerged biofilms on plastic and glass surfaces, macrocolony formation on agar plates,
and floating pellicle biofilms at an air–liquid interface (Danese
et al. 2000; Hung et al. 2013; Serra et al. 2013b). The vast array
of genetic tools that are available to work with E. coli has facilitated many molecular genetics-based studies of biofilm formation. E. coli has been shown to produce a range of autotransporter adhesins, the most studied of which is Antigen 43 (Ag43),
which acts to promote cell-to-cell adhesion and aggregation at
the initial stages of biofilm formation. In the mature biofilm, the
main conserved components of the E. coli biofilm matrix have
been defined as the proteinaceous curli fibres and flagella, alongside the polysaccharide cellulose. Additional components of the
biofilm matrix have been shown (in some strains) to include both
β-1,6-N-acetyl-D-glucosamine (PGA) and colanic acid (PrigentCombaret et al. 2000; Wang, Preston and Romeo 2004). Recent
advances in microscopy techniques have elucidated the internal
structure of the biofilm. Indeed using combinations of fluorescence light microscopy, transmission electron microscopy and
scanning electron microscopy (SEM), it was found that these extracellular components and appendages provide structure to the
biofilm and that they are found in discrete zones of the biofilm
(Serra, Richter and Hengge 2013a; Serra et al. 2013b).
AUTOTRANSPORTER ADHESINS
Cell-to-cell adhesion is mediated by a set of protein components
made by E. coli that are termed autotransporter adhesins. A wellstudied member of this class of proteins is the outer membrane
protein Antigen 43 (Ag43 encoded by agn43). Antigen 43 has been
shown to be responsible for aggregation of E. coli in stationary
liquid cultures (Henderson, Meehan and Owen 1997; Hasman,
Chakraborty and Klemm 1999). It was first shown to be important for biofilm formation in the E. coli strain W3110, where it
is required for wild-type levels of submerged biofilm formation
in glucose-minimal medium (Danese et al. 2000). An agn43 deletion strain retained the ability to attach to PVC surfaces, but the
biofilms were observed to be less dense than wild type when
quantified using crystal-violet staining. The single agn43 gene
encodes for a protein that is processed into two separate subunits, the α- and β-subunits (Hasman, Chakraborty and Klemm
1999): the β-subunit is an integral outer membrane protein that
is required for translocation of the α-subunit across the outer
membrane, whilst the α-subunit is found on the cell surface,
mediated through an interaction with the β-subunit. This autoaggregation of cells is dependent on Ag43–Ag43 interactions
(Hasman, Chakraborty and Klemm 1999). Solution of the protein
structure of Ag43 revealed the α-subunit to form an L-shaped
protein, and that pairs of α-subunits use a combination of
Hobley et al.
hydrogen bonds and salt bridges to stabilize intermicrobial protein dimers (Heras et al. 2014) which line up in a head-totail conformation, forming a protein ‘velcro’ that results in the
autoaggregation of cells. Expression of agn43, and hence the
production of Ag43, is under the control of phase variation,
which means that cells are either in an agn43 OFF or ON state
(Schembri et al. 2003; Chauhan et al. 2013). Biofilm formation
(as studied in the laboratory environment) positively selects for
cells in an agn43 ON state, and it has been hypothesized that
during host colonization the same selective pressure for agn43
ON cells will also exist (Chauhan et al. 2013). Antigen 43 has
been shown to be functional when expressed in other bacterial
species, in particular in Pseudomonas fluorescens, where it induces
cell aggregation and biofilm formation (Kjaergaard et al. 2000a,b).
It was also shown that when E. coli and P. fluorescens both express
Ag43 they are able to co-aggregate and effectively form mixed
species biofilms due to the interaction between Ag43 expressed
on the cell surfaces of the two different species (Kjaergaard et al.
2000a,b).
The subfamily of autotransporter adhesins to which Ag43 belongs also contains two other E. coli produced adhesins that can
enhance biofilm formation. The first, the autotransporter AIDAI (AIDA for short) is an adhesin that is produced by some diarrheagenic E. coli strains that in its glycosylated form can adhere to a variety of mammalian cells (Benz and Schmidt 2001).
AIDA is known to enhance cell aggregation and biofilm formation by E. coli, in a glycosylation-independent manner, through
intermicrobial AIDA–AIDA interactions (Sherlock et al. 2004). It
has been proposed that it is interactions between amino acids
with charged side chains that may be responsible for the AIDA–
AIDA interactions (Sherlock et al. 2004). It has also been shown
that AIDA can interact with Antigen 43, causing the formation of cell aggregates containing both AIDA-expressing cells
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and Ag43-expressing cells, and these interactions are also AIDAglycosylation independent (Sherlock et al. 2004). Another member of this autotransporter adhesion subfamily is TibA, which
is produced by some strains of enterotoxigenic E. coli strains.
Like AIDA, TibA is a glycoprotein, and glycosylation is essential
for adherence of the E. coli strains to human cells, but similarly
to the AIDA adhesin, glycosylation of TibA is not required for
the aggregation of E. coli cells, or the enhancement of biofilm
formation, caused by intermicrobial TibA–TibA interactions
(Sherlock, Vejborg and Klemm 2005). Knowledge of the molecular basis of these interactions will be important in the development of small molecules with the potential to block cell-to-cell
interactions during the infection process.
CURLI—THE MAJOR PROTEIN COMPONENT
OF THE BIOFILM MATRIX
The major protein constituents of the E. coli biofilm matrix are
the curli fibres. Escherichia coli thrives ex vivo and consistent with
curli-dependent biofilm formation occurring outside the host,
many isolates produce curli at temperatures below 30◦ C (Romling et al. 1998a). Curli are encoded for by two divergent operons,
csgBAC and csgDEFG (Hammar et al. 1995) (Fig. 1A). The csgBAC
operon encodes both components of the structural fibre, CsgA
and CsgB, and the accessory periplasmic protein CsgC. The csgDEFG operon encodes the regulator CsgD, which controls curli
and cellulose production, two putative accessory proteins CsgE
and CsgF, and the translocator protein CsgG.
Curli fibres are composed of two proteins: CsgB functions to
‘nucleate’ polymerization of the fibre and makes up a minority
component, whereas the majority of the fibre consists of CsgA
(Hammar et al. 1995; Hammar, Bian and Normark 1996; Bian and
Normark 1997) (Fig. 1A) (Table 1). The resulting curli fibres are
Figure 1. β-sheet-rich fibre formation by E. coli, B. subtilis and S. aureus. (A) Curli are amyloidous protein fibres assembled on the surface of E. coli cells within the nutrientdepleted zones of a biofilm and provide structural integrity. The curli fibre subunit CsgA is exported across the outer membrane through the CsgG translocator channel.
Once outside the cell, CsgA interacts with the CsgB nucleator protein, and polymerizes into amyloidous fibres that extend away from the cell. The accessory proteins
CsgC and CsgE regulate export by CsgG and CsgF is required for nucleation of CsgA by CsgB. (B) TasA-amyloid-like β-sheet-rich fibres protrude from the cell wall and
are required for biofilm formation. Biogenesis requires the products of the tapA-sipW-tasA operon. Each protein is made in the cytoplasm and transported across the
membrane by the Sec export system. SipW functions as a dedicated signal peptidase to cleave the signal peptide from TasA and TapA. TapA is required for anchoring
the fibres to the cell wall and forms a minor component in the β-sheet-rich fibres. TasA is the major component in the fibres. (C) PSM β-rich fibres are found elaborated
on the surface of S. aureus. The PSM transporter (PMT) is an ATP-dependent ABC transporter. It is composed of two transmembrane proteins (PmtB and D), coupled
with the ATPases PmtA and PmtC. PSMs are known to function in the monomeric state where their surfactant activity has cytolytic activity. The formation of the fibre
form is proposed to be a mechanism to inactivate the monomers until they are required again.
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FEMS Microbiology Reviews
Table 1. Functional amyloid/amyloid-like proteins in the biofilm matrix.
Bacterial species
Name of protein
Bacillus subtilis
TasA (TapA minor
component)
Enterobacter
cloacae
Curli (CsgA)
Escherichia coli
Curli (CsgA)
Pseudomonas spp.
Functional amyloid
pseudomonas (Fap)
fimbriae (FapC with
FapB as a minor
element)
Curli (alternatively Tafi
for thin aggregative
fimbrae) (CsgA)
Salmonella ssp.
Experimental evidence of
amyloid properties
Electron and atomic force
microscopy; Thioflavin T
and Congo red binding
propensity; CD spectrum
profile
Similarity at gene level to
csg operon; electron
microscopy of whole cells
Electron microscopy;
Thioflavin T and congo red
binding propensity; CD
spectrum profile; NMR
Electron microscopy; CD
spectrum profile; highly
stable protein fibres
Electron microscopy; highly
stable protein fibres
Staphylococcus
aureus
Phenol Soluble
Modulins
Electron microscopy;
Thioflavin T binding
propensity; detergent
resistant fibre
Streptococcus
mutans
Cell surface localized
antigen P1 (PAc)
Electron microscopy;
Thioflavin T and congo red
binding propensity;
detergent-resistant protein
fibres
rich in β-sheet structure and may be classified as ‘amyloid-like’,
although alternative structures such as the β-helix are also consistent with the structural data (Shewmaker et al. 2009). Fibres
bind the dyes Congo Red and Thioflavin T (ThT), which interact
predominantly but not exclusively with β-sheet rich structures
and aggregates (Khurana et al. 2001; Eisert, Felau and Brown
2006). The polymerized, fibrous form is highly resistant to denaturation and detergent solubilization (Collinson et al. 1991;
Hammar, Bian and Normark 1996; Chapman et al. 2002).
The CsgA protein sequence comprises three regions: the Secsignal peptide; an N-terminal 22 amino acid region of the mature
protein that does not form an integral part of the fibre, but is required for stability and secretion (Collinson et al. 1999; Robinson
et al. 2006; Nenninger et al. 2011); and the C-terminal domain that
forms the core of the β-sheet-rich fibre (Collinson et al. 1999).
This latter domain comprises five repeating units, predicted to
encode the β-strands. The first and fifth of the repeating units
of CsgA are essential for CsgA fibre-forming activity: when the
first (N-terminal) repeat is deleted, CsgA shows very little fibre
formation in vitro, whilst deletion of the fifth (C-terminal) repeat abolishes in vitro fibre formation completely (Wang, Hammer and Chapman 2008). In vitro, freshly purified His-CsgA is
Function within biofilm
References
Biofilm matrix component
Romero et al.
(2010); Chai et al.
(2012)
Biofilm matrix component
Zogaj et al. (2003)
Biofilm matrix component;
adhesion
Chapman et al.
(2002);
Shewmaker et al.
(2009)
Dueholm et al.
(2010, 2013)
Biofilm matrix component
Biofilm matrix component;
resistance to antibacterial
agents; adhesion to
surfaces
Biofilm matrix component;
biofilm stability; amyloid
formation blocks dispersal
activity of monomeric PSM
in vitro under specific
growth conditions; Not
identified to date if
synthesized in vivo
Biofilm matrix component
Collinson et al.
(1991); Collinson
et al. (1996)
Periasamy et al.
(2012); Schwartz
et al. (2012)
Oli et al. (2012)
unstructured and unpolymerized, whereas curli fibres purified
from E. coli are rich in β-sheet (Chapman et al. 2002).
CsgB has a similar overall domain structure to that of CsgA
but the fifth, C-terminal repeat is less conserved than the other
four, and additionally has a high proportion of positively charged
amino acids. Deletion of either the fourth or fifth repeat resulted
in a loss of outer membrane association and a corresponding
loss of CsgA polymerization (Hammer, Schmidt and Chapman
2007). Nonetheless, the truncated version of CsgB, missing just
the fifth repeating unit, was able to initiate CsgA polymerization
in vitro, indicating that only the first four repeating units are required for its nucleation activity (Hammer, Schmidt and Chapman 2007). Like CsgA, CsgB in its monomeric form is unstructured, and only in an oligomerized state does it adopt a β-sheet
rich secondary structure and enhance CsgA polymerization efficiency (Hammer, Schmidt and Chapman 2007). CsgB is associated with the outer membrane of E. coli, and in the absence of
CsgA, forms short fibres on the surface of the E. coli cell (Bian and
Normark 1997).
In vitro studies of the aggregation kinetics of CsgA, CsgB
and mixtures of CsgA and CsgB revealed distinct kinetics for
each curli component (Shu et al. 2012). In isolation, CsgB rapidly
Hobley et al.
switches from unstructured to β-sheet, coincident with the onset of fibre formation. In comparison, CsgA initially forms amorphous aggregates with very little β-sheet structure, with fibres
appearing only at later times. When CsgA and CsgB are mixed
in vitro, CsgB rapidly self-assembles into oligomers that accelerate CsgA aggregation and fibre formation. This same process
is thought to occur on the bacterial cell surface. Thus, current
models of E. coli have CsgB anchored to the cell surface, where it
nucleates the formation of CsgA fibres. Interestingly, CsgB is able
to nucleate the formation of curli fibres when the CsgA protein is
added exogenously (Wang, Hammer and Chapman 2008) or produced by other cells in the colony (Chapman et al. 2002). Thus,
coproduction of CsgA and CsgB in the same cell is not required.
CsgG forms a pore within the outer membrane of the E. coli
cell (Robinson et al. 2006), which is required for the secretion
of CsgA, CsgB and CsgF (Loferer, Hammar and Normark 1997;
Robinson et al. 2006; Nenninger, Robinson and Hultgren 2009;
Taylor et al. 2011). CsgG contains a core domain attached to two
adjacent transmembrane β-hairpins (Goyal et al. 2014). When
extracted from the membrane using detergents, CsgG assem˚ channel (Goyal et al. 2014),
bled into a nonamer forming a 40A
consistent with an earlier structural modelling approach that
had suggested a channel formed of between 7 and 9 proteins
(Taylor et al. 2011). A second structural analysis of CsgG once
more revealed the CsgG channels to be composed of nine
monomers, each of which contributed 4 β-strands to the final 36strand β-barrel making up the pore through which the curli subunits are exported (Cao et al. 2014). Three aromatic residues (Phe63, Tyr-66 and Tyr-71) were also identified as being important for
the selectivity of the CsgG channel for the export CsgA and CsgB,
and therefore are important for the assembly of functional curli
(Cao et al. 2014). In the presence of all the protein components
required for the formation of functional curli, CsgG forms foci in
cells that colocalize with the point at which the curli fibres are
anchored to the outer membrane (Epstein, Reizian and Chapman 2009). In the absence of any of the other components of
the csg system, or with non-polymerizing mutants of CsgA, CsgG
does not form foci and is instead dispersed around the cell (Epstein, Reizian and Chapman 2009). Overexpression of CsgG results in erythromycin sensitivity of the cells, but not vancomycin
sensitivity or growth defects in the absence of antibiotics, revealing that overexpressed CsgG forms discrete pores in the membrane and does not cause a general loss of membrane integrity
(Robinson et al. 2006).
The periplasmic protein CsgC is not required for curli assembly; however, csgC mutant strains exhibit defects in binding of Congo Red, loss of sedimentation (but not of aggregation) in static cultures and exhibit reduced binding to fibronectin
(Hammar et al. 1995). Studies of the homologous Salmonella curli
system Agf indicated that deletion of AgfC resulted in a different
tertiary structure of the main curli subunit protein AgfA, and an
absence of the nucleator protein AgfB (White et al. 2001; Gibson
et al. 2007) (Table 1). The crystal structure of CsgC revealed an
immunoglobulin-like fold with an invariant CxC motif in one of
the loops between two of the β-strands (Taylor et al. 2011). This
CxC motif placement was identified in another E. coli protein,
DsbD, a periplasmic redox-active protein. This led Taylor et al.
(2011) to hypothesize that CsgC may be involved in regulating
the redox state of C230 of CsgG, thereby regulating the export of
the fibre subunits and thus the formation of curli fibres.
CsgE is a periplasmic protein that functionally interacts with
CsgG to facilitate curli fibre formation. In the absence of CsgE,
CsgG forms an ungated pore through which erythromycin enters the cell and non-curli proteins are secreted (Nenninger et al.
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2011). This suggests that CsgE provides a gating mechanism to
prevent uncontrolled protein secretion and/or small molecule
uptake via the CsgG pore. The first 22 amino acids of CsgA,
previously shown to be sufficient to direct CsgA to the CsgG
translocon (Robinson et al. 2006), act as a specificity signal to allow translocation through the CsgG pore when gated by CsgE
(Nenninger et al. 2011).
CsgE has additionally been shown to prevent the selfassembly of CsgA into fibres in vitro (Nenninger et al. 2011),
thereby acting as a chaperone to prevent inappropriate selfassembly. When added to CsgA in vitro at any point during fibre formation, CsgE prevents further fibre formation or growth,
and surface plasmon resonance experiments indicated that this
is due to a direct interaction between CsgE and the CsgA fibres
(Andersson et al. 2013). Moreover, exogenously added CsgE inhibited the formation of floating, pellicle, biofilms, a process that
is dependent on the formation of curli fibres (Cegelski et al. 2009;
Andersson et al. 2013).
CsgF is required for nucleation of CsgA by CsgB, and for the
formation of curli fibres (Chapman et al. 2002). CsgF localizes to
the outer surface of the outer membrane, where it directly interacts with the translocator protein CsgG (Robinson et al. 2006;
Nenninger, Robinson and Hultgren 2009). Colonies formed by a
csgF− strain do not stain with Congo Red, and fewer fibres are
observed. The majority of the CsgA protein remains in an SDSsoluble, unaggregated, form (Chapman et al. 2002) that is not cell
associated (Nenninger, Robinson and Hultgren 2009). In a csgG−
strain, CsgF is undetectable from whole-cell samples, but neither of the curli fibre subunits CsgA and CsgB nor the accessory
protein CsgE was required for CsgF localization at the cell surface (Nenninger, Robinson and Hultgren 2009). CsgF enhances
the cell-surface localization of CsgB and has also been shown
to mediate the protease resistance of CsgB, which in turn is associated with the ability of CsgB to nucleate CsgA (Nenninger,
Robinson and Hultgren 2009). CsgF, like CsgA and CsgB, contains a high proportion of glutamine and asparagine residues,
and it has been hypothesized that these residues promote CsgB
structure formation, although this remains to be experimentally
tested (Nenninger, Robinson and Hultgren 2009).
Whilst the majority of the molecular studies on curli biogenesis and function have focused on those from E. coli (and
Salmonella), a bioinformatics-based approach has identified curli
homologs throughout the Proteobacteria, as well as in some Bacteroidetes, a single Firmicutes species, Halanaerobium and a
member of the Thermodesulfobacteria (Dueholm et al. 2012). In
addition to identifying homologs of the curli structural proteins
CsgA and CsgB, Dueholm et al. (2012) also analysed the conservation of the csgCAB and csgDEFG operon structures, showing that
whilst csgEFG were found in most of the bacteria with potential
CsgA/B homologs, the accessory protein CsgC and the regulator
CsgD were often missing. Whilst this bioinformatics approach
remains to be validated by in vivo analysis of the identified curli
homologs in other species, it does suggest that the formation
of curli-like fibres may be more widespread throughout biofilmforming bacteria than first thought, forming a common mechanism to provide structural integrity to the biofilm matrix. Analogous extracellular protein fibres involved in biofilm formation
are detailed in Table 1.
CELLULOSE
Cellulose is one of the most abundant organic polymers in nature (Ljungdahl and Eriksson 1985). It has a relatively simple
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FEMS Microbiology Reviews
Figure 2. Escherichia coli biofilm structure. After prolonged incubation, E. coli forms complex colony type biofilms on agar plates, where the three major components of
the biofilm matrix have been shown to be curli fibres, cellulose and flagella filaments. Left: the E. coli complex colony contains both concentric rings and axial wrinkles,
the rings are dependent on the production of both curli and flagella and the axial wrinkles additionally require the production of cellulose. The colony can be divided
into three zones (I) the outer edge, (II) the middle zone and (III) the inner region. Right: a cross-section of the colony shows the different cell types co-existing within
the biofilm, and their location within the different biofilm regions. Dividing, flagellated cells are found within the outer edge, whilst in the middle zone and the inner
region two distinct cell types are found. Near the agar surface post-exponential, rod-shaped cells are found encased in a mesh of flagella filaments, whilst in the upper
levels of the colony stationary phase, ovoid cells are found, these are surrounded in a dense mesh of curli fibres and cellulose.
structure, made from a (1 → 4)-β-linked linear glucose chain
and, while most frequently thought of in connection with the
plant cell wall, it can be synthesized by some species of bacteria where it has a protective, architectural and regulatory function during biofilm formation (Solano et al. 2002; Ude et al. 2006;
Gualdi et al. 2008). The role of cellulose as a component of the
biofilm matrix was first identified in Salmonella typhimurium,
in which the cellulose synthase gene cluster which is conserved between S. typhimurium and E. coli was initially identified
(Zogaj et al. 2001). In the same study, homologues of the cellulose gene cluster were noted in four different E. coli strains, three
of which were demonstrated to produce cellulose (Zogaj et al.
2001). Further analysis of cellulose production by E. coli revealed
that the proteins involved in cellulose synthesis are encoded in
two divergent operons: yhjR-bcsQABZC and bcsEFG (Zogaj et al.
2001; Solano et al. 2002; Le Quere and Ghigo 2009). The BcsA and
BcsB proteins form the two subunits of the cellulose synthase
complex that is located within the membrane and converts UDPglucose to cellulose (Zogaj et al. 2001; Omadjela et al. 2013).
While cellulose is clearly needed for biofilm formation (as
discussed further below), in the E. coli strain MG1655, overproduction of cellulose has a negative effect on submerged biofilm
formation (in 96-well plates and glass tubes) and on cell aggregation (Gualdi et al. 2008). It was proposed that, when overexpressed, cellulose may inhibit the formation of biofilm types that
are reliant on curli-mediated adhesion by masking the curli fibres by coating them in excessive cellulose (Gualdi et al. 2008).
Here, it was also revealed that overexpression of cellulose increased resistance of E. coli to desiccation, whilst deletion of the
gene encoding the major curli subunit CsgA resulted in a decrease in desiccation resistance. It was proposed that curli and
cellulose may work together to protect the E. coli against environmental stresses (Gualdi et al. 2008). A recent study has shown
that biofilm formation by E. coli protects the bacteria from predation by both the nematode Caenorhabditis elegans and by the
predatory bacterium Myxococcus xanthus, and that this protection is dependent on the presence of both cellulose and curli
(DePas et al. 2014).
A STRUCTURAL ROLE FOR THE FLAGELLUM
WITHIN THE DEVELOPING E. COLI BIOFILM
Escherichia coli is capable of forming architecturally complex
macrocolonies on agar plates (Romling et al. 1998b; Serra et al.
2013b) that are structurally reminiscent of the biofilms formed
by, for example, both B. subtilis (Branda et al. 2001) and Vibrio
cholerae (Yildiz and Schoolnik 1999). The molecular mechanisms
underpinning the macrocolony formation process have been
probed using genetically distinct strains of E. coli and architectural complexity has been proven to be provided by three components: the curli fibres and cellulose as described above, but
also the bacterial flagellum (Hung et al. 2013; Serra, Richter and
Hengge 2013a; Serra et al. 2013b). It was during analysis of the
macrocolony structures formed by the E. coli K12 strain W3110
that the structural role for the flagellum during biofilm formation was revealed. It is important to note that in strain W3110, as
with all widely used laboratory isolates of E. coli, cellulose is not
produced due to a point mutation in the bcsQ gene (Serra, Richter
and Hengge 2013a). The macrocolony formed by strain W3110
can be divided into three distinct regions: the outer edge, which
forms a narrow and smooth zone; a middle zone in which wrinkles initially emerge and which then becomes characterized by
the appearance of concentric rings; and the inner wrinkled region, corresponding to the area in which cells were first inoculated onto the plate (Fig. 2). Experimental work indicated that the
morphology of the developing biofilm was dependent on both
the production of the flagella filament and on the ability of the
flagella to rotate. Mutation of either fliC or motA (the flagellar filament protein and one of the motor proteins) rendered E. coli unable to make a fully ringed patterned colony (Serra et al. 2013b).
In contrast, analysis of mutants in the regulatory cascade that
govern flagella synthesis, flhDC or fliA, (which do not produce
flagella) resulted in macrocolonies with the ring structures but
which lacked wrinkles (Serra et al. 2013b). In the flhDC and fliA
deletion strains, there is an increase in the level of the secondary
messenger cyclic-di-GMP due to the loss of expression of phosphodiesterase YhjH which is under the control of both FlhDC
Hobley et al.
and FliA (Pesavento et al. 2008; Serra et al. 2013b). The increase in
cyclic-di-GMP levels results in increased curli production which
partially compensates for the absence of the flagellar filament,
presumably by fulfilling its structural role (Pesavento et al. 2008;
Serra et al. 2013b). Microscopy analysis of the internal structure
of the colony biofilm revealed that cells in the lower portion of
the biofilm, nearest the agar, were surrounded in a network of
flagella filaments, where it is hypothesized that the flagella filaments are acting as a structural component of the biofilm matrix
(Serra et al. 2013b). In a motA mutant strain, where the flagella are
unable to rotate, the mesh of flagella filaments were seen to be
less entangled, suggesting that flagella rotation is required for
effective tethering together of the cells and enables the formation of the mesh of flagella filaments at the base of the macrocolony (Serra et al. 2013b). These findings link the flagellar filaments with the overall architecture of the biofilm and reinforce
the regulatory role for flagellar biosynthesis in the coordination
of transcription of the other matrix elements.
Analysis of floating, pellicle, biofilms formed at the air–liquid
interface revealed a further requirement for flagella in biofilm
formation (Hung et al. 2013). Strains harbouring deletions of either the gene encoding the filament subunit FliC or the master
regulator FlhDC were severely attenuated in their ability to form
pellicle biofilms (Hung et al. 2013). Both strains formed small
‘rosette’ biofilms at the air–liquid interface. Analysis of the protein content of the wild-type pellicle biofilms revealed a complete absence of FliC protein, suggesting that flagella are not
used as a structural component in the pellicle form of biofilm,
in contrast to in the macrocolony (Hung et al. 2013; Serra et al.
2013b). This suggests that the main role of flagella in pellicle formation is in the initial aggregation of the cells at the air–liquid
interface. It is likely that in the natural habitat the requirement
for the flagellum is variable and will encompass physical propulsion, regulatory and architectural roles.
E. COLI PRODUCES STRUCTURAL
COMPONENTS OF THE MATRIX IN
DISCRETE ZONES OF THE BIOFILM
Insight to the spatial distribution of cells in the E. coli macrocolony came from a combination of SEM and fluorescence microscopy of cross-sections (Serra, Richter and Hengge 2013a;
Serra et al. 2013b). The images demonstrated that curli fibres and
flagella are synthesized in discrete zones of the biofilm, highlighting a bimodality that developed within an isogenic progenitor population (Serra et al. 2013b) (Fig. 2). At the upper surface of
the biofilm, the cells were small, ovoid and surrounded in an extracellular mesh of curli fibres (Serra et al. 2013b). By comparison,
the cells at the bottom of the macrocolony, nearer to the surface
of the agar plate, were longer and rod shaped. Again these cells
were encased in a dense mesh of filaments; however, in this case
the mesh was composed of flagella filaments (Serra et al. 2013b).
Zoning of cell types was also apparent horizontally through the
biofilm macrocolony (Fig. 2). The outer edge zone of the macrocolony contained long rod-shaped cells that were again tethered
together in a mesh of entangled flagella. Within the middle zone
of the macrocolony (the area in which the concentric rings form),
an interface was seen where cells encased in the mesh of flagella at the base of the colony gave way to smaller ovoid cells
surrounded in a dense mat of curli fibres in the upper regions.
The composition of the matrix mesh seems to correlate with the
metabolic state of the cells within that region of the biofilm. In
the outer edge zone of the macrocolony, and in the lower areas
7
nearer to the agar surface, the rod-shaped cells are in a state
of post-exponential growth and are still dividing, and are also
still able to produce flagella. In the upper, central zones of the
biofilms the cells have entered stationary phase due to nutrient
limitation, causing them to stop producing flagella, and instead
become smaller ovoid cells that produce curli (Serra et al. 2013b).
Restoration of cellulose production to the laboratory strain
of E. coli K12 W3110 changed not only the morphology of the
colony, but also its size and overall physical properties. The
colony formed by the dual curli- and cellulose-producing isolate was flat, large and showed a pronounced ‘tissue-like’ elasticity (Serra, Richter and Hengge 2013a). In the upper zones of
the biofilm, in the areas previously shown to consist of small
ovoid cells encased in a curli-fibre mesh, the addition of cellulose production resulted in the smooth coating of all the cells in
this region, suggesting that both cellulose and curli contribute
to the matrix structure. In the transition zone, between the stationary phase, curli-producing cells in the upper layer and the
post-exponential, flagella-producing cells in the lower layer, a
zone rich in cellulose was observed. In this transition zone, the
entangled flagella were observed to be acting as a scaffold for
cellulose fibre formation (Serra, Richter and Hengge 2013a). It is
relevant to note that cellulose can ‘wheel lock’ flagellar rotation,
thereby leading to an alteration in gene regulation that turns ‘off’
flagellar gene transcription (Zorraquino et al. 2013). This physically mediated method of impeding biogenesis of the flagellum
allows production of cellulose to be tightly linked with the onset
of motility inhibition.
The demarcation of different cell types in a stratified E. coli
biofilm has also been seen for the uropathogenic E. coli strain
UTI89 which forms floating pellicles at the air–liquid interface.
Using a combination of scanning and transmission electron microscopy, two distinct zones were identified within the pellicle
biofilm, the upper air-exposed side and the lower liquid-exposed
side (Hung et al. 2013). These two zones are reminiscent of those
identified in the macrocolony formed by E. coli K12 (Serra, Richter
and Hengge 2013a). At the upper, air-exposed, side of the pellicle, the (apparently ovoid) cells were covered in a matrix composed of very densely packed fibres that formed ‘basket-like’
structures surrounding the E. coli in the biofilm (McCrate et al.
2013; Serra, Richter and Hengge 2013a). At the base of the pellicle, nearest to the liquid, the cells were seen to be rod shaped
and loosely packed, with very little fibrous matrix around the
cells. As indicated previously, flagella were not detectable by
Western Blot analysis of the pellicular material, so all the fibrous
material within the matrix is likely to be formed from a combination of curli and cellulose (Hung et al. 2013). The insoluble
extracellular matrix material extracted from these biofilms has
been subjected to state-of-the-art solid-state nuclear magnetic
resonance (NMR) analysis, and the ratio (by mass) of the curli
fibres to cellulose was shown to be consistently approximately
85%:15% (McCrate et al. 2013).
Further analysis of the localization of matrix components,
and of the metabolic states of cells within the biofilm regions,
of the biofilms formed by other related Enterobacteriaceae will
reveal whether the mechanisms employed by E. coli in biofilm
formation are universally adopted strategies and will highlight
the in vivo relevance. Whilst the distinct cell types within the
biofilms are likely caused by the differences in nutrient availability across the biofilm, i.e. the post-exponential-phase cells
located nearer the nutrient source, and the stationary-phase,
curli and cellulose producing, cells further away from the nutrients, this diversification of cell fate may act as a protective
or bet-hedging solution allowing for a more rapid response to
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FEMS Microbiology Reviews
environmental changes or stresses as there already exist cells
in a range of metabolic states.
VIBRIO CHOLERAE
Vibrio cholerae is a Gram-negative halophilic bacterium with a
single polar flagellum that is a member of the family Vibrionaceae. It is the etiological agent of cholera, a disease which
results in severe, and often fatal, diarrhoea. The formation of
biofilm communities is important in the disease process from
both the angle of initial infection and transmission. The main
route for infection is the consumption of contaminated food or
water (Zuckerman, Rombo and Fisch 2007). It has been shown
that removal of particulate matter from water sources using
crude filtration methods that remove aggregates of bacteria or
biofilms significantly reduces the prevalence of cholera infection (Huq et al. 1996; Colwell et al. 2003). Consistent with these
findings, it has been shown that biofilm-like masses extracted
from the faeces of infected patients are more infectious than
planktonic bacterial counterparts (Faruque et al. 2006; Kamruzzaman et al. 2010). Here we will discuss recent advances in
the understanding of the structure and function of the extracellular polysaccharide (EPS) and protein components of the V.
cholerae biofilm matrix and highlight major novel experimental
approaches that have altered our perspective of how the biofilm
matrix is assembled.
COMPOSITION OF THE VIBRIO
EXOPOLYSACCHARIDE
The toolbox of molecular components used by V. cholerae to
form the biofilm matrix has been identified predominantly using classical bacterial genetic approaches (Yildiz and Schoolnik 1999). The major biofilm exopolysaccharide, dubbed VPS for
Vibrio exopolysaccharide, was first recognized during molecular
analysis of a rugose colonial variant of V. cholerae O1, biotype El
Tor (Yildiz and Schoolnik 1999). It was established that the rugosity of colony and pellicle morphology was directly correlated
with enhanced production of an EPS and that this additionally
increased resistance to chlorine, suggesting a protective function for the exopolymer (Yildiz and Schoolnik 1999). Since this
initial discovery, it has been demonstrated in various studies
that VPS is required for biofilm formation under many in vitro
conditions using pellicle and colony formation assays as indicators (Fong et al. 2010). Moreover, in support of biofilm formation
and VPS production being required for infection and biofilm formation in vivo, analysis using the rabbit ileal loop model system demonstrated poor biofilm formation by the vps mutant
(Kamruzzaman et al. 2010). Further to this, colonization of the intestine of Drosophila melanogaster after oral ingestion of V. cholerae
occurs in a VPS-dependent manner, although the physiological
relevance remains to be determined given that Drosophila is not
a natural vector for transmission (Purdy and Watnick 2011). It
is important to note, however, that V. cholerae has the capacity
to form two types of biofilms in the laboratory; one that is dependent on VPS and another that is VPS independent and triggered by concentrations of Ca2+ found in seawater (Yildiz and
Schoolnik 1999; Kierek and Watnick 2003a,b). These findings are
compatible with a scenario where in the natural environment
the VPS is required for biofilm formation in some environmental conditions while not in others and is likely compensated for
by the proteinaceous components that are discussed below.
Building on the identification of VPS as an important component of the biofilm matrix, a necessary prelude to chemical
disruption of biofilms using small molecules to disrupt or digest
the VPS is detailed knowledge of the structure. The genes that
participate in the biosynthesis of the V. cholerae exopolysaccharide are found in two clusters on the chromosome (Fong et al.
2010). Cluster vps-I consists of the genes vpsU (VC0916) and vpsAK (VC0917–27) and cluster vps-II carries the vpsL-Q (VC0934–9).
Using a systematic mutagenesis approach, it was identified that
the single mutant strains fell into six phenotypic classes with
the majority of the 18 genes in the two clusters being involved
or required for VPS production, and therefore biofilm formation (Fong et al. 2010). Initial compositional examination of isolated VPS suggested an approximately equal contribution from
glucose and galactose monomers, with linkage analysis detecting a similar amount of 4-linked galactose and 4-linked glucose, suggesting that they formed the backbone of the molecule
with branching of 3,4- and 4,6-linked galactose and glucose and
2,4-linked galactose forming the side chains (Yildiz and Schoolnik 1999). However, solution-state NMR analysis has recently
elucidated the structure of the polysaccharide component of
the VPS and in doing so established the presence of an additional sugar monosaccharide (namely 2-acetamido-2-deoxy-Lguluronic acid) in the polysaccharide (Yildiz et al. 2014). These
findings expand the chemical space of molecules used in Vibrio biofilm biology and demonstrate the complexity of understanding the nature of the exopolysaccharides found in the matrix. Additional analysis indicated that the VPS isolated from the
biofilm matrix appears bound to an additional as yet unidentified component that increases the solution viscosity and thereby
suppresses any solution NMR signal (Yildiz et al. 2014). After
acid hydrolysis, the polysaccharide component released was
amenable to NMR spectroscopy. It will be of interest to define
the interaction between the VPS and the unknown molecule
and establish if this interaction plays a critical role in biofilm
matrix assembly and integrity. Moreover, it is clear that further work will be required before targeted enzymatic digestion
of the VPS in biofilm aggregates can be used as a therapeutic
approach.
The study of VPS in Vibrio highlights the strength of new
technology. For example, an advance in the understanding of
the molecular composition of the V. cholerae biofilm matrix has
been achieved using novel solid-state NMR methodologies (Reichhardt et al. 2014). This new approach avoids the necessity to
treat samples with harsh chemicals that are needed for other
forms of analysis such as MS/MS analysis of carbohydrate composition. The new atomic-level information of the biofilm matrix
of V. cholerae has provided a molecular fingerprint in a largely unprocessed state allowing the assignment of the carbon pool to
sugar, lipid and protein pools thereby conclusively demonstrating that, unlike the rugose E. coli biofilm (McCrate et al. 2013),
the majority of the biofilm matrix is in carbohydrate form (Reichhardt et al. 2014). The development of this technique adds to
the pool of methodologies that allow an analysis of the macromolecules in the extracellular matrix in situ (Nichols et al. 1985;
Ivleva et al. 2008, 2010; Lanni et al. 2014; Neu and Lawrence 2014).
It is of interest to note that these methods of analysis are entirely
compatible with molecular investigations of the impact of specific genetic mutations, the analysis of mixed species and complex natural communities and additionally could shed light on
the molecular composition of the biofilm matrix formed by genetically intractable species.
Hobley et al.
9
PROTEIN COMPONENTS IN THE V. CHOLERAE
BIOFILM MATRIX
The biofilm matrix of V. cholerae, like the majority of studied
bacterial species, also contains extracellular proteins that contribute to biofilm structure and integrity. There are three main
proteins involved in the V. cholerae biofilm matrix structure and
integrity namely Bap1, RbmA and RbmC. Each of these proteins is synthesized with an N-terminal signal peptide and is
secreted into the biofilm matrix by the type-II secretion system
(Johnson et al. 2014). Consistent with an extracellular localization, proteomic analysis of V. cholerae outer membrane vesicles
(OMV) has reported that RbmA, RbmC and Bap1 are OMV proteins. Furthermore, incorporation of the proteins into the OMVs
was shown to be dependent on the protease DegP (Altindis,
Fu and Mekalanos 2014). Here we will discuss what is known
about the structure and function of each of the aforementioned
proteins.
Bap1 (for biofilm-associated protein 1) was initially uncovered using a global transcriptomic analysis designed to identify
genes that were differentially expressed between biofilm- and
monolayer-attached cells (Moorthy and Watnick 2005). Bap1 is
encoded by locus VC1888 and is coregulated with the vps-island
(Moorthy and Watnick 2005). Bap1 has been linked with antimicrobial resistance in a process currently thought to be independent of its role in biofilm formation (Duperthuy et al. 2013).
Bap1 is a large protein (691 amino acids) and detail regarding
the mechanism of Bap1 is limited, and knowledge is largely restricted to that gleaned from bioinformatic analyses (Fig. 3A).
This has identified the presence of multiple FG-GAP domains
(Fong et al. 2006; Absalon, Van Dellen and Watnick 2011) and a
β-prism domain in the sequence (Fig. 3A), but the function of
these domains is currently unknown. Recent biophysical analysis has linked Bap1 with controlling the mechanical strength of
pellicle biofilms and the overall hydrophobicity of the air–liquid
interface structures. These data were generated using interfacial rheology to measure the elasticity of pellicle biofilms and
contact angle measurements to assess the surface properties
(Hollenbeck et al. 2014). These findings place Bap1 in the same
functional category as proteins such as BslA of B. subtilis (vide
infra) (Kobayashi and Iwano 2012; Hobley et al. 2013).
RbmC (for rugosity and biofilm structure modulator C) is 957
amino acids in length and exhibits 54% identity and 70% similarity to Bap1 (Absalon, Van Dellen and Watnick 2011). Consistent with this high level of homology, Bap1 and RbmC are functionally redundant during biofilm formation. Like Bap1, RbmC
also contains multiple FG-GAP domains and additionally harbours two lectin-binding domains, suggestive of carbohydratebinding capability (Fig. 3A). Deletion of the C-terminal lectinbinding (β-prism-like) domain of RbmC, that is absent from
Bap1, indicates that this domain is dispensable for function (Absalon, Van Dellen and Watnick 2011). These findings thereby
support the data indicating that the two proteins can functionally compensate for each other but leave open the question
of which regions of the protein are mechanistically important.
Despite the apparent functional redundancy, high-resolution
image analysis documenting the timing of protein production
and localization in the biofilm matrix has highlighted differences in the deployment and localization of the two proteins
(Berk et al. 2012).
RbmA (for rugosity and biofilm structure modulator A) is
needed for biofilm rugosity and detergent resistance (Fong et al.
2006). It is a 271-amino-acid protein that is synthesized with
a 30-amino-acid Sec-dependent signal peptide (Fig. 3A) and is
Figure 3. Structure and deployment of V. cholerae biofilm matrix proteins. (A)
Bioinformatics analysis of RmbC (accession number Q9KTH2), RmbA (accession
number Q9KTH4) and Bap1 (accession number Q9KQW0) was performed de novo
using a combination of SMART (Schultz et al. 1998; Letunic, Doerks and Bork
2014), InterPro (Hunter et al. 2012) and BLAST (Johnson et al. 2008) to identify
conserved domains, SignalP (Petersen et al. 2011) to designate signal sequence
peptide cleavage sites and where required further information was revealed using WU-BLAST analysis (http://www.ebi.ac.uk/Tools/sss/wublast/). For Bap1, it
should be noted that the EF-hand domain has a low confidence score of 7.00e-03
and for RmbC the integrin α-N-terminal domain had a confidence value of 5.00e04 so the presence of these protein domains should be interpreted with caution.
The domains and proteins are drawn approximately to scale. Parts B and C are
reproduced, with permission, from Berk et al. (2012) Science along with the corresponding legend. Images are pseudo-coloured blue (cells), grey (RbmA), red
(RbmC) and green (Bap1). RbmA localizes around and within cell clusters. RbmC
and Bap1 encase cell clusters. Cells were counterstained with DAPI. Scale bars,
3 μm. (B) 3D biofilm architecture. (C) Enlargement of the boxed region in (B). Red
arrow indicates one cell cluster. Red signal now rendered partially transparent
to allow visualization of cells within an RbmC-containing cluster.
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FEMS Microbiology Reviews
secreted into the extracellular environment via the type II secretion system (Johnson et al. 2014). RbmA was initially identified using proteomic analysis as increased in production within
rugose biofilms (Fong et al. 2006) and consistent with a role in
biofilm formation it is encoded in a region of the large chromosome along with the vps-gene clusters. Atomic-level information regarding RbmA function has been gleaned from the recently solved structure of the mature domain (Giglio et al. 2013).
Here it was elucidated that RbmA forms a homodimer where
each monomer comprises two tandem fibronectin type (FnIII)
domains (Fig. 3A). Biological significance for the dimer observed
in the crystal structure was determined biochemically using
size exclusion chromatography and small angle X-ray scattering
(Giglio et al. 2013). Analysis of the tertiary structure identified significant homology with several proteins including GpbA from V.
cholerae which is an N-acetylglucosamine (GlcNAc)-binding protein, a human transglutaminase and a dextranase from Streptococcus mutans (Giglio et al. 2013). Based on the knowledge that
some of the structurally related proteins are known to bind
carbohydrates, structure-guided site-directed mutagenesis was
used to isolate functionally active domains of the protein. The
importance of a surface-exposed strong positive electrostatic
groove for rugose biofilm formation was identified, and it was
postulated that this region was required for interactions with
other matrix components, such as the VPS, to facilitate the function of RbmA as a scaffold in the biofilm matrix (Giglio et al.
2013). This is a strong hypothesis that is consistent with the
knowledge that protein–carbohydrate interactions are required
for biofilm stability and formation. One example that falls in
this class is CdrA which is an extracellular β-helical filamentous protein synthesized by P. aeruginosa that interacts with an
exopolysaccharide called Psl to consolidate the matrix (Borlee
et al. 2010). As a multivalent adhesion, CdrA has the potential
to interact with more than one component presumably thereby
strengthening the cross-links within the biofilm matrix and the
range of interactions possible with a host.
NOVEL IN SITU IMMUNOFLUORESCENCE
ANALYSIS OF PROTEIN LOCALIZATION
Building on the knowledge of the proteins found in the V. cholerae
biofilm matrix, exciting data showing where and when the proteins are localized within the architecture of the biofilm have
recently been derived using immunofluorescence imaging technologies (Absalon, Van Dellen and Watnick 2011; Berk et al.
2012). Initial work using single colour immunofluorescence microscopy identified that RmbA was localized throughout the
entire biofilm whereas Bap1 was restricted to the base of the
biofilm where it mediates adhesion of neighbouring ‘bystander’
cells (Absalon, Van Dellen and Watnick 2011). Subsequently,
greater resolution of the timing and position of deployment was
achieved by employing powerful high-resolution four-colour immunofluorescence confocal microscopy. As with the single protein analysis method, this approach required the use of a genetically tractable organism. Modification of the coding regions
for RmbA, RmbC and Bap1 was required so that each incorporated a unique immunoreactive epitope tag. The production of
the epitope-tagged proteins was subsequently followed, in real
time, during biofilm formation using multi-resolution confocal
microscopy. This showed that each of the components in the
biofilm matrix had complementary roles that, in combination,
facilitated the assembly of the biofilm. The first protein that was
detected was RmbA, which was found in discrete sites on the
cell surface and allowed daughter cells to remain attached to
the surface. Next was Bap1, which localized to the junction between two daughter cells promoting retention of the new cell
and coated the surrounding surfaces. Finally, RmbC was detected at discrete sites on the cell surface which, over time, developed into a layer that encapsulated clusters of cells alongside the VPS and Bap1 (Berk et al. 2012) (Fig. 3B and C). It will be
of interest to establish if similar patterns of protein behaviour
are observed for other species allowing classification of the proteins in the biofilm matrix into functional subgroups in the
matrix.
BACILLUS SUBTILIS
Bacillus subtilis is a Gram-positive endospore forming soil
bacterium that is used to study the molecular mechanisms of
bacterial development. Commercially, B. subtilis and the closely
related species B. amyloliquefaciens are known as plant growthpromoting bacteria that can protect plants from pathogenic microorganisms in a manner dependent on biofilm formation (Bais,
Fall and Vivanco 2004; Kloepper, Ryu and Zhang 2004; Nagorska,
Bikowski and Obuchowski 2007). It was initially reported that
B. subtilis had the capacity to form what are now defined as
‘biofilms’ back in the 1870s by Burton-Sanderson and Ferdinand
Cohn (Cohn 1877; Vlamakis et al. 2013); however, it was only
in the early 2000s that investigations into the molecular basis
of biofilm formation were initiated. The work started with the
identification of the capability of environmental isolates of B.
subtilis to form rugose colonies and pellicles that were subsequently shown to contain subpopulations of isogenic differentiated cells (Fig. 4A) (Branda et al. 2001; Vlamakis et al. 2008;
Marlow et al. 2014). At the same time it was noted that laboratory isolates of B. subtilis had lost the capability to form
rugose biofilms (Branda et al. 2001) but retained the capacity
to develop submerged surface-adhered biofilms (Hamon and
Lazazzera 2001). The survival and propagation of B. subtilis in the
biofilm is dependent on the production of an extracellular matrix. The matrix is required for the projection of environmentally
resistant spores on aerial structures at the surface of the biofilm
(Branda et al. 2001; Veening, Hamoen and Kuipers 2005), wrinkle and internal channel formation (Wilking et al. 2013), complex colony spreading (Seminara et al. 2012), extreme hydrophobicity (Epstein et al. 2011) and mechanical stiffness (Asally
et al. 2012).
The B. subtilis biofilm matrix predominantly comprises a
large molecular weight soluble secreted polysaccharide, for
which there is limited compositional knowledge (Branda et al.
2001; Chai et al. 2012; Jones et al. 2014), and extracellular proteins
called TasA and TapA (Branda et al. 2006; Romero et al. 2011).
The production of the aforementioned extracellular molecules
is not sufficient to accomplish biofilm maturation, which does
not occur unless the protein BslA (Biofilm surface layer protein
A) (originally called YuaB) is synthesized (Ostrowski et al. 2011). It
is noteworthy that in line with biofilm matrix production being
energetically expensive, the expression of each of the operons
needed for matrix biosynthesis is subject to tight transcriptional
control. The regulatory pathways that control transcription and
the current knowledge of the EPS structure and function have
recently been reviewed (Cairns, Hobley and Stanley-Wall 2014;
Mhatre, Monterrosa and Kovacs 2014; Mielich-Suss and Lopez
2014). Thus, here we focus on the current understanding of the
mechanisms underpinning the production and function of each
of the protein matrix components.
Hobley et al.
11
Figure 4. Bacillus subtilis biofilm formation. Biofilm formation by B. subtilis culminates in the formation of a structured highly hydrophobic sessile community. The
isogenic population differentiates to divide tasks within the community. For a detailed review of this process refer to (Cairns, Hobley and Stanley-Wall 2014; MielichSuss and Lopez 2014).
A NOVEL BACTERIAL HYDROPHOBIN IS USED
TO MAKE A BIOFILM RAINCOAT
BslA is a bacterial hydrophobin that is needed for the observed
architectural complexity of the rugose wild-type biofilm and for
the formation of a highly hydrophobic barrier that encases the
bacterial community (Kobayashi and Iwano 2012; Hobley et al.
2013). Consistent with this function, by using both immunofluorescence and epifluorescence microscopy, BslA was found to
form a coat or layer around the mature biofilm (Kobayashi and
Iwano 2012; Hobley et al. 2013). BslA production is highly regulated at the level of transcription (Kobayashi 2007; Verhamme,
Murray and Stanley-Wall 2009; Kovacs and Kuipers 2011) and
functions cooperatively with the TasA/TapA and exopolysaccharide components of the matrix to expedite biofilm maturation (Ostrowski et al. 2011; Kobayashi and Iwano 2012; Hobley et al. 2013). BslA is synthesized with a 28-amino-acid Secdependent signal peptide and, after signal peptide cleavage the
16.4 kDa mature protein is released into the extracellular environment (Ostrowski et al. 2011). In the wild-type biofilm, the
mature processed BslA can function as a ‘communal good’ benefiting non-producing cells (Ostrowski et al. 2011). Experimental evidence is suggestive of BslA stability and localization being
dependent on the exopolysaccharide component of the biofilm
matrix (Kobayashi and Iwano 2012). Consistent with this, the extreme non-wetting phenotype of the mature biofilm was first
correlated with successful production of the large secreted EPS
synthesized by the products of the 15-gene epsA-O operon. Indeed, the non-wetting nature of the wild-type biofilms is entirely
abrogated upon deletion of the epsA-O gene cluster (Epstein et al.
2011). However, as the B. subtilis biofilm EPS is water soluble it
seems unlikely that it is directly responsible for the non-wetting
phenotype of the mature biofilm. It is possible that the EPS mediates hydrophobicity indirectly as a consequence of currently
undefined interactions with BslA. We have previously hypothesized a further possible role of BslA: since the channels through
the centre of the biofilm wrinkles allow the passage of liquid and
it is possible that BslA may coat these channels allowing for the
rapid movement of liquid into the inner regions of the biofilm
(Cairns, Hobley and Stanley-Wall 2014). The function of BslA, and
the knowledge that the mature BslA protein can be shared with
non-producing cells in a mixed co-culture biofilm, supports the
definition of B. subtilis biofilms as social, cooperative, bacterial
communities.
THE MECHANISM OF BslA ACTIVITY
A partial explanation of how BslA functions was derived from in
vitro experiments using purified recombinant BslA. Tensiometry
demonstrated that the recombinant BslA, while soluble in solution, was surface active meaning that the protein migrates to
an air/water or oil/water interface where it forms an elastic film
(Hobley et al. 2013). The crystal structure of BslA48–171 provided
atomic level insight to the surface activity and revealed the presence of a highly hydrophobic ‘cap’ domain with nine exposed
leucine and isoleucine residues displayed on an immunoglobulin IgG-like scaffold (Hobley et al. 2013). Based on these findings,
BslA was named a ‘bacterial hydrophobin’ in line with the terminology used to classify the proteins that coat fungal fruiting bodies and aerial hyphae (Elliot and Talbot 2004). It should be noted,
however, that BslA does not exhibit sequence or structural similarity to the fungal hydrophobins. It is clear that the analysis of
the biophysical and molecular mechanisms underpinning the
assembly of matrix components such as BslA will have implications for understanding how to disrupt bacterial biofilms.
TasA FIBRE FORM AND FORMATION
The production and assembly of amyloid-like fibres in the B.
subtilis biofilm matrix is accomplished by the proteins encoded
within the tapA-sipW-tasA operon (Branda et al. 2006; Romero
et al. 2010) (Fig. 1B) (Table 1). TasA is the major protein component of the extracellular fibres and, consistent with this, deletion of tasA blocks the formation of a robust, rugose biofilm both
in vitro and in planta (Branda et al. 2006; Beauregard et al. 2013).
Biofilm formation on plant root surfaces has been linked with
biocontrol properties exhibited by B. subtilis (Bais, Fall and Vivanco 2004) and, reflecting the biofilm defect exhibited by the
tasA mutant, it confers reduced protection against pathogen attack compared with the wild-type strain (Chen et al. 2013). Intriguingly, in laboratory isolates of B. subtilis, deletion of tasA
is not associated with a decrease in surface-adhered biofilm
formation suggesting that TasA is not needed for submerged
biofilm formation (Branda et al. 2004; Hamon et al. 2004). The experimental basis for this behaviour remains to be elucidated.
TasA is synthesized with a 27-amino-acid Sec-dependent signal peptide. The 25.7 kD mature protein is released into the extracellular environment after signal peptide cleavage by SipW
(Stover and Driks 1999b; Terra et al. 2012) (Fig. 1B). Here it is
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FEMS Microbiology Reviews
assembled into fibre form in a TapA-dependent manner (Romero
et al. 2010). The first biological function assigned to TasA (originally called CotN) was as a spore-associated protein that exhibited broad spectrum antimicrobial activity (Stover and Driks
1999b). It has been speculated that the antimicrobial activity associated with TasA may help provide protection to the
cells within the biofilm (Romero et al. 2010). The propensity of
TasA to form fibres was initially discovered using electron microscopy analysis coupled with immunogold-labelling detection
techniques (Romero et al. 2010). It was also noted that TasA fibres could be purified from the extracellular milieu of B. subtilis cultures and that they were able to bind Thioflavin T and
Congo red (Romero et al. 2010); as discussed above, these characteristics are consistent with (but not evidential of) amyloid
fibre formation (Khurana et al. 2001; Eisert, Felau and Brown
2006). Secondary structure analysis highlighted a high proportion of β-strand content in the purified protein fibres (Romero
et al. 2010) (Table 1). After this initial analysis, further biochemical and biophysical experiments indicated that TasA was purified in an oligomeric state in solution (Chai et al. 2013) and that
fibre formation was stimulated over time in vitro by environmental conditions that included hydrophobic surfaces, such as electron microscopy grids (Romero et al. 2010) and acidic solutions
(Chai et al. 2013). As the authors note, these findings raise the
fascinating question of what triggers TasA fibre formation in the
natural environment of the biofilm. It is interesting to speculate
that the hydrophobic properties of the biofilm raincoat protein
BslA might be involved.
FACILITATING FIBRE ASSEMBLY
AND ATTACHMENT BY TapA
Assembly of TasA fibres in the biofilm is dependent on TapA
(renamed from YqxM for TasA anchoring/assembly protein)
(Romero et al. 2011). The mature assembled TasA fibres serve
as a ‘common good’ in the biofilm matrix that are capable
of being shared with non-producing cells (Branda et al. 2006).
This has been determined using both biochemical and genetic
approaches that supply TasA oligomers from the extracellular environment. However, consistent with an intimate working
relationship between TasA and TapA during the elaboration of
functional TasA fibres from the cell body, synthesis of these two
proteins needs to occur in the same cell (Romero et al. 2011). For
example, co-culture of the tasA and tapA mutant cell lines does
not allow formation of a wild-type biofilm (Romero et al. 2011).
This is in contrast to the curli subunits CsgA and CsgB produced
by E. coli, where CsgB acts as a nucleator (like TapA) and CsgA is
the major fibre component (like TasA). In this system, CsgA and
CsgB can be produced by separate cells and CsgB will still act as
a nucleator and facilitate polymerization of CsgA into fibres in
the exogenous environment (Chapman et al. 2002). It will be of
interest to understand the molecular reasons for this difference.
TapA is a 253-amino-acid protein that is synthesized with a
33-amino-acid Sec-dependent signal sequence cleaved upon secretion by SipW, a dedicated signal peptidase (Stover and Driks
1999a). TapA is predominantly found anchored to the cell wall
but also forms a minor component (1:100) of the TasA fibres
(Romero et al. 2011, 2014) (Fig. 1B). There is very little molecular
data regarding TapA function, and primary sequence bioinformatics analysis yields limited information. However, the presence of five conserved cysteine residues and of two regions each
containing two imperfect repeat sequences was recently noted
(Romero et al. 2014). The cysteine residues were shown only to
have a limited impact on TapA function; in contrast, disruption
of the N-terminal imperfect repeat by either deletion or replacement of amino acids 50–57 (TFDVSLQT) impaired biofilm formation, although the protein was still seen to localize on the
cell surface. Furthermore, the variant TapA protein displayed a
dominant negative effect on wild-type TapA function, and inhibited TasA polymerization in vitro (Romero et al. 2014). It is possible that the deletion of the first N-terminal repeat inhibits TasA
polymerization, but does not inhibit either TapA cell-surface localization or incorporation into the fibres, thereby inhibiting the
onset of fibre polymerization in vivo and also halting in vitro fibre formation at high ratios of TapA to TasA. It is clear that while
our understanding of TasA/TapA function is growing, our knowledge base would strongly benefit from atomic-level structural
data that may help to inform the existence of any interactions
that exist between the two proteins and indeed the other matrix molecules. Furthermore, the ability to form biofilms in the
laboratory has been correlated with the ability of some B. subtilis
isolates to persist within the gastrointestinal tract of mice, and
whilst a definitive cause and effect has not yet been shown it
has been hypothesized that biofilm formation may be responsible for the persistence of germinated spores after ingestion (Tam
et al. 2006). It will be of interest to understand how (and if) the
extracellular macromolecules interact with the host gut environment and commensal intestinal flora.
STAPHYLOCOCCUS AUREUS
Staphylococcus aureus is a major human and live-stock-associated
pathogen that is capable of causing diseases ranging from superficial skin infections to life-threatening sepsis. To achieve
this, the organism has acquired diverse mechanisms to colonize and evade the host immune response. Of these mechanisms, the formation of biofilms is particularly problematic
given the impermeability of the bacterial communities to the
host immune cells and antibiotic therapy (Costerton, Stewart
and Greenberg 1999). Staphylococcus aureus biofilm infections
have been directly linked with infective endocarditis or implantassociated infection, causing persistent and destructive diseases which are a massive burden in respects to both morbidity and mortality (Costerton, Stewart and Greenberg 1999;
Jones et al. 2001). Both clinically and in the laboratory, the
molecules that aid biofilm formation by S. aureus are strainspecific and, although the mechanism remains unclear, are
noted to vary between methicillin-sensitive and methicillinresistant (MSSA/MRSA) lineages (O’Neill et al. 2007; Geoghegan
et al. 2013).
Broadly speaking, Staphylococcal biofilm accumulation can
be viewed as: ica-dependent, relying upon polysaccharide intercellular adhesion production; dependent on the high molecular
weight poly-N-acetyl-β-(1–6)-glucosamine (PNAG) which is synthesized by the products of the icaADBC operon (Cramton et al.
1999; Maira-Litran et al. 2002); or ica-independent. Indeed, while
the ica operon is present in most S. aureus isolates its expression varies in a strain and growth condition-dependent manner (Cramton et al. 2001). Transcription of the icaADBC operon
has been shown to be controlled by phase variation, with PNAGnegative strains identified as possessing a growth advantage
over the PNAG-producing siblings (Ziebuhr et al. 1997, 1999;
Brooks and Jefferson 2014). Upon production of the PNAG polymer, in addition to aiding biofilm formation, the bacteria gain
protection from the host immune system, thereby attaining an
additional growth advantage (Cerca et al. 2007).
Hobley et al.
13
Figure 5. Staphylococcus aureus biofilm formation. Attachment of S. aureus to a surface is mediated by CWA proteins. Cell-to-cell interactions occur during accumulation
phase and can be mediated by several factors. The magnified region shows this in more detail: (1) extracellular DNA linking recycled cytoplasmic proteins; (2) CWA
proteins binding adjacent cell surfaces; (3) Homophilic interactions between CWA proteins. PSMs form amyloid-like fibres visible at the surface of the biofilm. They
also act in the formation of channels within the biofilm to allow nutrient access, while their surfactant properties aid the dispersal phase. The different stages of
biofilm formation are detailed from left to right across the diagram.
There are currently two models for PNAG biosynthesis and
secretion by S. aureus (Atkin et al. 2014); the first is as follows:
IcaA as an GlcNAc transferase needed for the synthesis of the
PNAG polymer where the function of IcaA is dependent on the
integral membrane protein IcaD (Gerke et al. 1998). IcaB is an
N-deacetylase that is specific to PNAG (Pokrovskaya et al. 2013).
This leaves IcaC, which is an integral membrane protein with
multiple transmembrane domains that is thought to play a direct role in PNAG export. The second, more recently, postulated
mechanism of PNAG biosynthesis and export places an IcaAD
membrane complex responsible for the export of PNAG with
IcaC functioning to add succinyl groups to the growing polymer
using its O-succinyltransferase activity (Atkin et al. 2014). Either
way, a greater understanding of the biosynthesis and structure
of PNAG is likely to be of therapeutic benefit during the treatment of ICA-dependent biofilm infections. In ica-independent
biofilms, proteinaceous factors are utilized to allow biofilm development, a phenotype which appears to be more prevalent
in MRSA isolates (O’Neill et al. 2007). Here, we focus on the
cell-wall-anchored (CWA) and secreted proteins that promote
biofilm formation by this important pathogenic species of bacteria.
CELL WALL ANCHORED PROTEINS
In S. aureus, the expression of CWA proteins is integral to the
ability of the organism to attach to a surface and thereby initiate
biofilm formation (Fig. 5). At the molecular level, CWA proteins
are characterized by the presence of a Sec-dependent secretory
signal sequence at the N-terminus and contain an ‘LPXTG’ motif at the C-terminus (Fig. 6A). Upon secretion, the proteins are
cleaved by Sortase A (SrtA), a membrane-bound transpeptidase,
which catalyses the attachment of the processed protein to the
cell wall peptidoglycan (Ton-That et al. 1999). S. aureus encodes
up to 24 different CWAs and there can be significant variation in
expression between strains and in a growth condition-specific
manner (Foster et al. 2014). For example, strain ‘Newman’ is a
Figure 6. Structure of the CWA proteins. All CWA proteins contain a Secdependent secretory signal sequence, a C-terminal LPXTG sortase motif, a hydrophobic domain and finally a chain of positively charged residues at the end
of the C-terminus. (A) The schematic demonstrates the typical domain structure within the MSCRAMM family of CWAs. At the N-terminus, a Sec-dependent
signal sequence followed by a variable number of binding domains that begin
with an N-terminal A domain (inclusive of N subdomains in the case of FnBPs (B
and C) and ClfB). These are followed by a wall-spanning region, the LPXTG motif and finally a membrane-spanning region. (D) In SasG, the binding domain is
subdivided into the N-terminal A domain and a varying number of G5/E repeats.
clinical isolate that forms weak biofilms, most likely as it carries
mutations in the coding regions for the Fnbp proteins (vide infra)
(Grundmeier et al. 2004). To date, the CWA family of proteins has
been the most extensively studied with respect to their function
as biofilm-associated factors that bind ligands on cell surfaces
to allow adhesion.
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FEMS Microbiology Reviews
The CWA proteins that have been demonstrated to be of importance during in vitro and in vivo biofilm development include
the biofilm-associated protein (Bap) (Cucarella et al. 2001), the
fibronectin-binding proteins A/B (FnbpA/B) (O’Neill et al. 2008;
Geoghegan et al. 2013), clumping factor B (ClfB) (Abraham and
Jefferson 2012), serine-aspartate repeat protein C (SdrC) (Barbu
et al. 2014), S. aureus surface protein C (SasC) (Schroeder et al.
2009), S. aureus surface protein G (SasG) (Geoghegan et al. 2010)
and protein A (Merino et al. 2009). It should be noted that each of
these proteins, with the exception of Bap, has been detected in
human clinical isolates, while Bap has been found in bovine isolates only (Cucarella et al. 2001). The proteins bind to eukaryotic
extracellular matrix proteins, or to surfaces which have been
primed by host plasma such as medical implants (Lower et al.
2011).
LIGANDS OF THE CWA PROTEINS
Several of the CWA proteins have known ligands, and it has been
shown that they can have overlapping targets or in fact more
than one target for binding. For instance, the clumping factors
A and B (ClfA/B) (Deivanayagam et al. 2002; Ganesh et al. 2011)
and fibronectin-binding proteins A/B (FnbpA/B) each bind the
plasma protein fibrinogen in a specific manner (Wann, Gurusiddappa and Hook 2000; Burke et al. 2011). Additionally, a number
of the CWAs have ligands which are epidermal proteins. For example, ClfB is known to bind the epithelial cell envelope protein
Loricrin aiding nasal colonization (Mulcahy et al. 2012), while
iron-regulated surface protein A (IsdA) binds to cytokeratin 10
in desquamated human epithelial cells (Clarke et al. 2009). It is
conceivable that the functional overlap with respect to ligand
binding has developed as a means to maximize the chances of
S. aureus adherence. Selective pressures within the host have
also presumably driven the development of body site-specific
ligands, e.g. Loricrin or Cytokeratin, as a way of enabling attachment. These processes of adaptation within the CWA proteins
appear to be continual, and may play a role in the outcome of
infection. For instance, it has been shown that bacterial isolates
extracted from cardiac device implants have single amino acid
polymorphisms within the fibronectin-binding domains. These
amino acid level changes result in higher ligand-binding affinity
to fibronectin than found in isolates from bacteraemia or nasal
isolates (Lower et al. 2011).
While CWAs can be classified on the basis of structure, detail regarding the molecular mechanism by which S. aureus uses
CWA proteins during biofilm formation remains limited in many
cases. For instance, Protein A has been classed structurally as
a three helical bundle protein, containing five homologous IgGbinding domains (Graille et al. 2000). When it is produced at high
levels on the cell surface, Protein A is able to promote biofilm formation, but how aggregation of the cells is triggered is unknown
(Merino et al. 2009). Other CWAs remain structurally uncharacterized, for example SasC which has been shown to contribute
to intercellular adhesion and biofilm formation (Schroeder et al.
2009), although its role at the molecular level remains to be defined. A common theme is that lack of insight of the CWA protein ligands is limiting our understanding of their function. Use
of techniques such as phage display has elucidated that SdrC
(classified as a MSCRAMM, see below) homophilic interactions
in S. aureus are a mode of cell aggregation in biofilm formation
(Barbu et al. 2014). This technique could be a prospective means
for identifying new ligands for other CWA proteins, to allow in-
roads to potential routes of blocking/disrupting intercellular interaction and biofilm formation.
RELATING STRUCTURE TO FUNCTION
IN THE CWA PROTEINS
CWA proteins can be subdivided into categories according to
their structural and ligand-binding features. Many CWA proteins
bind to the extracellular matrix and these proteins have generally been termed Microbial Surface Components Recognizing
Surface Adhesive Matrix Molecules (MSCRAMMs). It has recently
been proposed that this acronym be used to specifically describe
a family of surface proteins which share structural and ligandbinding features (Foster et al. 2014) (Fig. 5). First, we will consider
the MSCRAMMs that include the proteins ClfA/B, FnbpA/B and
SdrC among others (Foster et al. 2014). They are characterized
by an N-terminal signal sequence (Sec), N-terminal A domain
with 2 or more IgG-like domains (Deivanayagam et al. 2002) and
a C-terminal LPXTG sortase recognition sequence (Fig. 6B and
C). ClfA is the classically used example of an MSCRAMM protein. The fibrinogen-binding domain A of ClfA has been characterized in crystal form, and based upon this, structural predictions suggest that the other MSCRAMMs have highly similar A domain structures (Deivanayagam et al. 2002). The fibrinogen and fibronectin-binding capability of ClfA has been localized
to the N-terminal A domains (Hartford et al. 2001). This binding
capability allows S. aureus to adhere to the extracellular matrix
to initiate biofilm formation. For the Fnbp’s, functional assessment of the A domains has demonstrated that the fibrinogenbinding A subdomain is sufficient to promote biofilm formation,
with the fibronectin-binding domains being dispensable (O’Neill
et al. 2008). Further analysis of mutations within the N1, N2 and
N3 subdomains of the A domain have demonstrated that N3
is required for FnbpA to bind to fibrinogen, whilst N2 and N3
mediate accumulation of the biofilm (Fig. 6B and C). It remains
mechanistically unclear how Fnbp proteins mediate accumulation, but is hypothesized to involve homophilic interaction of
N2–N3 domains on sibling bacteria (Geoghegan et al. 2013). The
MSCRAMM ClfB is known to bind fibrinogen, but this function
is distinct from its ability to mediate cell aggregation which appears to be calcium dependent (Abraham and Jefferson 2012).
It will be necessary to identify the binding sites responsible for
these proposed cell-to-cell interactions, not least as they could
provide potential means of disrupting and clearing biofilms mediated by MSCRAMMs.
SasG, A FIBRE FORMING CWA PROTEIN
As detailed above for E. coli and B. subtilis, biofilm formation by
S. aureus requires the production of fibre-forming proteins. SasG
is a CWA protein that aids binding of bacterial cells to desquamated nasal epithelial cells (Roche, Meehan and Foster 2003).
Structurally SasG is classified as a member of the GE repeat family (Foster et al. 2014). It forms (non-amyloid) β-sheet-rich protein
fibrils that protrude from the cell surface, which can be visualized by electron microscopy (Corrigan et al. 2007). The SasG fibrils
have been proposed to represent a mechanism by which individual bacteria can become linked together during the attachment
phase of biofilm formation (Geoghegan et al. 2010; Foster et al.
2014). With respect to protein domain organization, SasG follows the typical pattern of the LPXTG proteins where, following
the N-terminal A domain in the mature protein, there are multiple tandem GE repeats (Fig. 6D). Within these repeats, sequences
Hobley et al.
contain G5 domains of approximately 80 residues, which are followed by 50 residue sequences known as E regions (Gruszka et al.
2012) (Fig. 6D). It is specifically the GE repeat regions of the SasG
protein that have been identified as being required for the accumulation of biofilms (Geoghegan et al. 2010). Mechanistic insight
to SasG function has been derived from analysis of the crystal
structure of the EG5 domains which was found to be composed
of a single layer of β-sheets. Interlocking connections between
the E and G5 domains of SasG leads to the formation of rod-like
protein structures which are responsible for protein fibrils on the
cell surface (Gruszka et al. 2012).
PHENOL SOLUBLE MODULINS
A group of peptides that have been recently discovered to be
of importance in both the development and stabilization of
biofilms formed by S. aureus are phenol soluble modulins (PSMs)
(Table 1) (Figs 1C and 5). Discovered originally in S. epidermidis as
immunomodulatory peptides, it is now well accepted that the
proteins confer virulence to S. aureus (Cheung et al. 2014). These
small peptides have an α-helical amphipathic structure which
is responsible for the surfactant-type properties they display in
their monomeric state (Wang et al. 2011; Periasamy et al. 2012).
The proteins are highly conserved across S. aureus strains, and
are encoded for at three distinct regions within the genome. The
regions include the alpha operon which expresses αpsm1–4, the
beta operon expressing β1 and 2, and RNAIII which is a regulatory RNA responsible for the expression of delta hemolysin
(Mehlin, Headley and Klebanoff 1999; Wang et al. 2007). Transcription of all three operons is under the control of the accessory gene regulator (Agr) system (Queck et al. 2008).
The role of PSMs during biofilm formation was assessed by
direct comparisons of wild-type biofilm formation with that of
the psm and agr mutants. Analysis demonstrated that PSMs are
essential at multiple levels and that their absence prevented the
normal maturation of the biofilm. More specifically, PSMs are required for structuring of the biofilm where they have direct involvement in the formation of channels through which nutrients can be obtained, dissemination of cells from the biofilm,
and expansion of the biofilm (Periasamy et al. 2012). PSMβ peptides appear to have the most pronounced impact on biofilm
structuring (Periasamy et al. 2012). These findings are particularly interesting if we consider the prolific biofilm-forming S. epidermidis. Although capable of producing all of the PSMs, S. epidermidis predominantly produces the poorly cytolytic PSMβ in culture (Wang et al. 2011). It may therefore be the case that PSMβ
has evolved specifically to function in structuring of the biofilm.
As with many virulence factors in Staphylococci, PSM production is dependent on both nutrient availability and growth
state (Schwartz et al. 2012). It was through investigation of the
effect of growth media on biofilm matrix composition that led
to the discovery that PSMs form fibrillar structures in S. aureus
biofilms (Schwartz et al. 2012). Growth of S. aureus in peptone–
NaCl–glucose media produced biofilms resistant to dispersal. In
these biofilms, hair-like fibres were visible using transmission
electron microscopy, but notably absent in agr/αβpsm mutants
that were prone to dispersal. Intact fibres conformed to traits
attributed to amyloid proteins including SDS insolubility, staining with amyloid-specific markers and β-sheet structure (Gebbink et al. 2005). Subsequent purification of the fibres revealed
several peptides covering the PSM family. Current thinking is
that the formation of these fibrils is a means of storing toxic
PSMs until the additional functions as antimicrobials and vir-
15
ulence factors are required (Schwartz et al. 2012). As the most
recently described group of biofilm-associated peptides/fibres,
many aspects of their biology remain to be uncovered. Further
elucidation of the process by which PSMs form amyloid-like
fibres and if they exist in vivo will be important to establish.
Additionally, given the cytolytic properties of the PSMs, which
lead to bacterial cell death, and their specific export system
(Chatterjee et al. 2013) (Fig. 1C), inhibition of export is an attractive prospective area of study in the search for anti-infective
agents. Whether small molecules targeted towards this system
could be harnessed as a possible means to destroy Staphylococci
during biofilm formation/infection remains to be established.
THE MATRIX OF S. AUREUS BIOFILMS
Emerging as additional possible components of the extracellular matrix of S. aureus biofilms are recycled cytoplasmic proteins
(Foulston et al. 2014). Using a proteomics approach, cell surface
proteins derived from biofilm (14 N) or non-biofilm (15 N) growth
conditions were biotinylated and analysed comparatively. A total of 11 proteins were found to be significantly enriched in
biofilm growth conditions, all of which were predicted cytoplasmic proteins with functions in metabolism. Through multiple approaches, including live/dead cell staining, it was demonstrated that these cytoplasmic proteins were released from live
cells during the development of the biofilm and remained cell
associated. With SDS-PAGE analysis, it was shown that the
proteins are retained at the cell surface of biofilm cells at a
pH of 5, and reversibly released when the pH increased again.
The pH-dependent mechanism of secretion of these cytoplasmic proteins has not been elucidated; however, the authors hypothesized that the cytoplasmic proteins coating the cell surface could carry a net positive charge which would act to link
to eDNA, and contribute to the biofilm matrix (Foulston et al.
2014). These findings provide a new and interesting mechanism
by which an organism can adapt to form a multicellular community depending on environmental conditions. Further interrogation of these mechanisms is certainly justified given that hyperglycaemic states (and thus low pH) are both a risk and a feature
of chronic biofilm infections such as diabetic ulcers (James et al.
2008).
EMERGING TRENDS AND FUTURE
DEVELOPMENT AREAS
The recognition of biofilms as the major reservoir of bacteria
within the natural environment, and the subsequent realization of the importance of biofilm formation to the survival and
pathogenicity of a vast number of bacterial species has spurred
the growth in interest and research into the molecular mechanisms underlying biofilm formation. The focus on a select group
of model organisms has revealed several conserved themes that
appear to underlie biofilm formation in all species. The first and
foremost is that the biofilm matrix is not simply a ‘slime’ that
surrounds the cells. It is in fact a highly ordered structure, with
extensive amounts of protein localization, and interactions between components, that result in a robust and protective biofilm
‘coat’. The structural components of the matrix are predominantly a mixture of polysaccharides and proteins, and many
of these proteins have been shown to polymerize into higherorder structures. For example, the curli fibres made by E. coli and
other Enterobacteria, the TasA fibres and bacterial hydrophobin
BslA, both produced by B. subtilis, and the PSM and the SasG
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β-sheet-rich fibres of S. aureus are all ordered protein aggregates.
It is interesting to speculate that one of the proteins required
for biofilm formation by V. cholerae may be found to form higherorder structures in the future.
The adaptation of various immunofluorescence microscopy
techniques has been used to study many of the biofilms discussed in this review. For instance, curli and cellulose localization in E. coli biofilms was analysed by staining the curli and
cellulose with Thioflavin S (Serra, Richter and Hengge 2013a),
the localization of the hydrophobin ‘raincoat’ protein BslA in
Bacillus biofilms was studied using antibodies specific to BslA
combined with fluorescent secondary antibodies prior to confocal microscopy (Hobley et al. 2013) and the localization of the
protein components of the V. cholerae biofilm was studied using
high-resolution four-colour immunofluorescence confocal microscopy (Berk et al. 2012). For the first two techniques, cryosectioning of the biofilms was used prior to imaging, allowing for
analysis of only a single point in the course of biofilm formation.
In contrast, the final technique was adapted for use in real time,
such that the founding bacterial cells could be tracked during the
course of formation. With advances in microscopy, it is probable
that imaging within a growing colony biofilm may soon be possible, in a manner similar to that already employed for the submerged surface-attached Vibrio biofilms. Super-resolution microscopes also exist such that single molecules within a sample
can be imaged. Applying this form of microscopy to the analysis of both the protein and polysaccharide components of the
matrix (for example using fluorescently labelled amino acids or
sugars) may reveal further detail as to how these two major matrix components interact and provide the incredible structural
integrity to the biofilm. Similarly, the advances in in situ NMRbased approaches may also reveal further information about the
structure of the polysaccharide components as well as revealing
the quantitative ratios of each component.
By ultimately understanding the mechanisms by which the
biofilm builds and maintains its structural integrity, the biofilm
matrix may become the target of future antimicrobial drug design. Biofilms are known to be a harbour for many antibioticresistant pathogens, and it is only by targeting the biofilm that
the most effective drugs will be produced. For instance, significant effort is directed at the development of novel vaccines for
S. aureus through utilising the fundamental knowledge of the
polysaccharide and adhesins needed for biofilm establishment
(Jansen et al. 2013). It is however important to keep in mind that
not all biofilm-forming bacteria are pathogenic species. Biofilm
formation is known to be essential for some beneficial functions,
for example B. subtilis can act as a plant protectant agent when
it grows on plant roots (Chen et al. 2013; Gao et al. 2013). When
colonizing plant roots, biofilm formation is essential for efficient
surface coverage, and the protein matrix component TasA and
the exopolysaccharide have both been shown to be essential for
effective plant root colonisation in both Arabidopsis and Tomato
plants (Rudrappa et al. 2007; Beauregard et al. 2013; Chen et al.
2013). It is possible that by further understanding biofilm formation by such species, we can design treatments that actually
promote or enhance biofilm formation on the plant root, leading
to a more effective plant protectant agent. Given that biofilm research is still in its (comparative) infancy, when compared with
the study of planktonically grown cells, our advances in understanding the complex mechanisms that result in the formation
of these multicellular communities have been immense. The
advances in microscopy and spectroscopy techniques and the
merging of expertise between disciplines that is currently underway make the future of biofilm research very exciting!
ACKNOWLEDGEMENTS
We thank Drs. Robert P. Ryan, Daniel Lopez, Diego O. Serra and
Prof. Fitnat Yildiz for valuable comments on the content of the
manuscript.
FUNDING
This work was supported by Engineering and Physical Sciences Research Council grant [EP/J007404/1] and Biotechnology
and Biological Sciences Research Council grants [BB/L006979/1;
BB/I019464/1; BB/L006804/1]. CH is funded by a clinical doctoral
fellowship grant from the Wellcome trust [104241/Z/14/Z].
Conflict of interest. None declared.
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