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More Agile, More Flexible; More Gene
Regulation Research Solutions
Epigenetics • Gene Expression • miRNA Expression
Agilent provides the most complete
solution for your gene regulation studies.
• Flexible, customizable
designs and formats
• Most updated content
• Highest sensitivity
and best accuracy
Focus: Agilent Epigenetic Analysis Solutions
Agilent’s epigenetics analysis solutions offer a wide range of tools to study
DNA methylation and histone modifications, as well as the role of noncoding
RNAs in regulatory pathways. Agilent provides the tools needed to gain a better
understanding of epigenetic control mechanisms that play a role in cancer,
human diseases, and cell development.
DNA Methylation and Histone Modifications
Study DNA methylation and histone modifications to understand how these
epigenetic modifications affect regulatory pathways, such as those known
to repress transcription of tumor suppressor genes and other human diseases.
Agilent Epigenetics Solutions
DNA Methylation
and Histone Modification
• SureSelect Methyl-Seq
• SurePrint DNA Methylation
and CHiP-on-Chip Microarrays
• SureCycler PCR
• 2100 Bioanalyzer
• GeneSpring NGS
Biofomatics Software
miRNA
•
•
•
•
SurePrint G3 miRNA Microarrays
Mx QPCR System and Reagents
2100 Bioanalyzer
GeneSpring GX
Bioinformatics Software
Key Products for DNA Methylation and Histone Modifications
SureSelectXT Human Methyl-Seq
The first comprehensive target enrichment system to enable researchers to focus on the
regions where methylation is known to impact gene regulation: CpG islands, CpG island
shores, undermethylated regions, promoters, and differentially methylated regions (DMRs).
• Uniquely delivers more information than methylation microarrays
by detecting individual CpGs
• Increases throughput and reduces costs compared to whole genome
bisulfite sequencing. (See figure 1 for correlation data.)
• Reveals methylated regions not detected by restriction enzyme
and immunoprecipitation or SNP-based assays
• GeneSpring NGS now supports Methyl-Seq, data for identifying methylation
sites generated SureSelectXT Human Methyl-Seq. (See figure 2.)
SurePrint DNA Methylation and ChIP-on-Chip Microarrays
Agilent’s catalog and custom DNA Methylation and ChIP-on-chip microarrays
enable analysis of the epigenetic events that regulate gene expression.
SureSelect XT Human
Methyl-Seq Results
MC7_GCCAA
MC7_TGACC
100
794072
Figure 2. GeneSpring NGS allows you to easily visualize Methylation results. Lollipop Plot shows
methylated C’s as circles colored by intensities relative to their methylation level or p-values.
miRNA
miRNAs are short noncoding RNAs known to regulate gene expression
post-transcriptionally. miRNAs also control expression of some epigenetic
regulators and are involved in an epigenetics-miRNA regulatory circuit
that when disrupted, may be involved in cancer development and other
human disease processes.
Key Products for miRNA
4.5
4.0
80
% Methylation Data
793925
3.5
3.0
60
2.5
2.0
40
1.5
1.0
20
0.5
0
0
20
40
60
80
100
% Methylation Reference
Figure 1. Methyl-Seq achieves excellent correlation
with whole genome bisulfite sequencing (WGS)
data (R=0.93, Lister R. et al. 2009).
SurePrint miRNA Microarrays
Agilent’s miRNA microarrays enable comprehensive miRNA expression
across a broad dynamic range, ensuring the detection of low and high
expressors to confidently study the role of miRNAs.
Other Products
PCR
SureCycler 8800 and PfuTurbo Cx Hotstart DNA Polymerase
QPCR
Mx QPCR Systems and Reagents
Sample QC
2100 Bioanalyzer and 2200 TapeStation Systems
Data Analysis
Software
GeneSpring GX and NGS Bioinformatics Software
For More Information Visit:
agilent.com/genomics/generegulation
Scan the QR code with
your smartphone for
more information
about Gene Regulation
www.agilent.com/genomics/generegulation
Agilent Products are for Research Use Only.
Not for use in diagnostic procedures.
Information, descriptions, and specifications in this
publication are subject to change without notice.
© Agilent Technologies, Inc. 2013
Published in USA, January X, 2013
5991-1641EN
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