Curriculum vitae Pavan Kumar Ph. D Boyce Thompson Institute for Plant Research Cornell University 533 Tower Road Ithaca, NY 14853 USA fp248@cornell.edu Educational qualifications Doctorate in Molecular Ecology (December 2013) Department of Molecular Ecology, Max Planck Institute (MPI) for Chemical Ecology, Jena Germany Thesis: Revealing Manduca sexta's nicotine metabolism and its ecological consequences using plant mediated RNAi based reverse genetics. Supervisor: Prof. Ian T. Baldwin Master of Science (MSc) Biotechnology (April 2005) Department of Biotechnology, Gulbarga University Gulbarga, India Bachelor of Science (BSc) Biotechnology, Chemistry and Zoology (April 2003) Karnataka College, Gulbarga University, Bidar, India Research interests Plant mediated RNAi, xenobiotic detoxification by insect pests and plant productivity. Research experience Post doctorate fellow Boyce Thompson Institute for plant research, Ithaca NY USA April 2014- present Project 1: Investigating molecular adaptation of potato plant overcompensation response to increase tuber yield Project 2: Improving nutritional quality of potato tubers Senior research fellow National Center for Cell Science (NCCS) Pune, India February 2008- January 2009 Project: Role of matrix associated binding protein SMAR1 in transcriptional regulation of genes Project assistant September Plant Molecular Biology Unit, National Chemical Laboratory (NCL) Pune, India September 2005- January 2008 Project: Identification and characterization of plant proteinase-inhibitor insensitive lepidopteran insect Helicoverpa armigera’s midgut proteases Publications 1. Difference in nicotine metabolism of two herbivores of Nicotiana attenuata renders them differentially susceptible to a common native predator. Kumar P, Matthias Schoettner, Pandit SS, and Baldwin IT. PLoS One 2014, 9(4), e95982. 2. A natural history driven, plant mediated RNAi based study reveals CYP6B46’s role in a nicotine-mediated anti-predator herbivore defense. Kumar P, Pandit SS, Steppuhn A, and Baldwin IT. Proc Natl Acad Sci USA 2013, 111: 1245-1252. Press coverage http://news.sciencemag.org/biology/2013/12/scienceshot-breath-so-bad-it-could-save-yourlife#disqus_thread http://phenomena.nationalgeographic.com/2013/12/30/toxic-halitosis-protects-tobacco-eating-caterpillar/ 3. 4. Tobacco Rattle Virus vector: A rapid and transient means of silencing Manduca sexta genes by plant mediated RNA interference. Kumar P, Pandit SS, Baldwin IT. PLoS One 2012 7(2), e31347. (Press: https://rybicki.wordpress.com/tag/sirna/) Spatial and temporal expression patterns of diverse Pin-II genes in Capsicum annuum Linn. Tamhane VA, Giri AP, Kumar P, Gupta VS. Gene. 2009, 442:1-2 (88-90 Awards Gold medal for BSc on 22nd annual convocation of Gulbarga University, India (2004). R. V. Bidap higher education scholarship from Karnataka College (K.R.E.S Society) Bidar, India to pursue master degree (2004). Merit student fellowship during MSc from Gulbarga University, India (2005). Rank student in MSc Biotechnology (2005). DAAD doctoral fellowships to pursue PhD degree in Germany (2009). Projects MSc thesis: Identification and characterization of glutamate decarboxylase in sugarcane (Saccharum officinarum .L) var.co.740 under salt stress. Department of Biotechnology, Gulbarga University Gulbarga, India (2005). Supervisor: Prof. G. R. Naik. BSc Thesis: Bio processing of organic wastes, for improved quality and quantity of bio-gas production, as an efficient source of fuel. Department of Biotechnology, Karnataka College Bidar, India (2003). Supervisor: Dr. Vijay Kumar Biradar Technical skills Molecular biology: Virus Induced Gene Silencing Gene cloning in bacterial and yeast cells Primer designing, PCR and qRT-PCR Northern, Southern and Western hybridizations Expression and purification of recombinant proteins from heterologous systems (E. coli and yeast) Animal cell culture and transfection Lab rearing and maintenance of lepidopteran insect cultures Analytical chemistry: HPLC (reverse phase and chiral), LC-MS (UPLC/ESI-TOF and Q3-MS) and GC-MS Field work: Studied N. attenuata- M. sexta interactions at Great Basin Desert Utah, USA (2012-13) Scientific soft skills: Basic bioinformatics tools for data search, retrieval and analysis StatView, ACD labs and MS office Language proficiency English (Fluent) Kannada (Mother tongue) Hindi, Marathi, Telugu (Fluent) German (Moderate) Qualified B1 level from EURSIA institute Berlin, Germany (2009) Oral presentations Plant mediated RNAi in lepidopteran insects, Karnataka College, Bidar India (February 2012) Probing insect response to nicotine by plant-mediated RNAi 10th IMPRS symposium Dornburg, Germany (February 2011) Detoxification of diterpenoid glycosides by M. sexta at Castle Ringberg Kreuth, Germany (February 2012) Poster presentations Manduca sexta larvae co-opt plant defense metabolite nicotine to deter spiders. Kumar P, Pandit S, and Baldwin IT 12th IMPRS Symposium, MPI for Chemical Ecology Jena, Germany (April 2013). Plant mediated RNA interference for the reverse genetics of a native insect herbivore in the field. Pandit S, Kumar P, Kessler D, and Baldwin IT, Scientific advisory board meeting, MPI for Chemical Ecology Jena, Germany (October 2012). Manduca sexta’s nicotine metabolism is influenced by the environment. Kumar P, Pandit S, and Baldwin IT. 11th IMPRS symposium, MPI for Chemical Ecology Dornburg, Germany (February 2012). Plant mediated RNA interference for insect gene silencing. Kumar P, Poosakkannu A, Pandit S, et al. Scientific advisory board meeting, MPI for Chemical Ecology Jena, Germany (October 2010). Sweet poison sours caterpillar’s life. Kumar P, Pandit S, and Baldwin IT. 9th IMPRS symposium, MPI for Chemical Ecology Dornburg, Germany (February 2010). Conference Poster: Identification and characterization of glutamate decarboxylase in sugarcane (Saccharum officinarum .L) var.co.740 under salt stress”. Babu G, Kumar P and Naik G R, at International conference on Plant genomics on biotechnology: Challenges and opportunities. Indira Gandhi Agricultural University Raipur, India (October 2005). Workshop At MPI for Chemical Ecology Jena, Germany Analysis of small molecules (January 2012) Advanced course on analytical chemistry (February 2011) Life after PhD; funding gates (March 2011) Advanced molecular cloning (November 2011) Proteomic insights into plant-insect interactions. Max-Planck society-India partnership program. NCL, Pune India (December 2006) Basic techniques in molecular biology and immunology at Institute of Biosciences and Molecular Biology (IBMB) and Bangalore Genei Pvt. Ltd., Bangalore, India (February 2005)
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