1. health and fitness
2. disease
3. aids and hiv

Newsletter 1. 2015
Kem-en-tec Nordic
Competent BioScience since 1987
Improve your NGS
- 100 % conversion rate
Mirus Transfection reagents
- Non Toxic!
ELISA - We are the experts
- Reagent and kits
- Mini workstation and washer
- Multilabel readers
High Throughput DNA Electrophoresis
- without gels!
Liquid handling
- Easy and Fast
Centrifuge/vortexer
- For DNA prep work
- Patented unique solution
Nordic
Contact info:
Finland
+358 9594822
Sweden/
Norway +46 18 149000
Denmark +45 39271777
info@kem-en-tec.com
All prices are valid until
30. June 2015.
Local currency exclusive
VAT and Ex Works.
ELISA – Mini
workstation
ELISA plate
washer
Set it up with your protocol and
leave it to dispense – incubate –
wash and finally read your plate.
Programmable 8-channel washer with
weight logger module for automatic
level control
Try it - We offer
FREE demo in
your own lab.
Campaign price
30.000,- DKK · 38.500,- SEK
36.000,- NOK · 4050,- €
Campaign price
For both round and
square well plates
- 8-well Strips and
whole 96 well format
155.000,- DKK · 198.750,- SEK
186.750,- NOK · 20.950,- €
Hoefer quality
at 25% discount
The golden standard in solutions
for Electrophoresis - Save 25 % on
ALL Hoefer orders.
Package example –
Request specific quote
2
Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777
Take advantage
of our spring
offer!
ELISA
Substrate –
ECO-TEK™
- Safe and easy
Kem-En-Tec ECO-TEK™ TMB
substrates
High quality ELISA­kits
for your research
eg Hormones & Steroids
Research ELISA Test Kits:
Hormones & Steroids
Reduce your exposure to carcinogenic reagents
Ready-to-use ELISA substrates - So Easy
Androstenedione
Corticosterone
Ordering: (Cat # 4380A)
Cortisol
1 litre of TMB ONE ready to use HRP
Substrate
Estradiol
DKK
Testosterone
SEK NOK
Estriol
€
Progesterone
1.760,-2.260,-2.125,- 240,-
Try in your ELISA
– Request
FREE SAMPLE
ECO-TEK™ products are
environmentally-friendly
components
Spring offer
Get 10 %
on ALL kits
Eicosanoids
Leukotriene B4
Leukotriene C4
Leukotriene C4/D4/E4
Prostaglandin E2
Prostaglandin E2 (Monoclonal)
Prostaglandin F2α
6-keto-Prostaglandin F1α
11β-Prostaglandin F2α
13,14-dihydro-15-ketoProstaglandin F2α
Thromboxane B2
11-dehydro-Thromboxane B2
Oxidative Stress
Lipoxins
Histamine
Cytokines
Specialty
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
3
Copenhagen LabMed
Booth no 6
·
t
e
d
e
t
s
k
r
komotivvæ
Working
What’s on display?
with
Plates?
Lo
Easy & Fast
liquid handling
and ­reading
Capillary
electrophoresis
Fragment Analyzer – the
perfect tool for your
DNA and RNA analysis
Multimodel Microplate Reader
HIDEX Sense
Molecular biology
• Big Touchscreen
• Pre-programmed applications
• Small footprint
• Extremely easy to use
• Beta-counting/LSC
• 1-1536 wells
- ELISA and many
other applications
See the newest clever
gadgets for visualization
of DNA
See in the video how quick
and easy you get your results.
The world’s
most efficient
polymerases
Plate handling
Experience the Kapa
Biosystems solutions
first hand
CyBi®-FeliX
CyBi®-SELMA
• With 96 and 384 channel pipetting heads
• Precise 96 or 384 channel pipetting with
the possibility to work in single wells,
rows and columns
• Interchangeable pipetting heads
• Electrophoresis
Very small footprint
• Semi-automatic 96- or
384-fold pipettor with
minimal required space
• Easy changing of tips
• Remembers height of
plates!
and Fractionation
MW protein fractionator GelCyBio•AG
Free8100 – An ideal pre MS analysis
20 years
of
tool
German
• RunBlue SDS-PAGE – Precast gels with
Qualitysuperior resolution and so strong
that they show no tears!
• InstantBlue – The 15 min Coomassie –
Quick and Easy gel staining
See the video
ELISA
• Crocodile ELISA mini workstation
New Tristar² S
Multilabel reader with
monochromator- great
introduction discounts
Request
– Run your ELISA for you
• Plate washers 8 and 96 channel
references
– Also good for Plate coating
ELISA Readers & automation –
and•demo!
LED based solutions
• ELISA Reagents – Ready to use
and safe
Lab
instruments
See and try the
hot new laboratory instruments
from BioSan
See the video
4
Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777
October 7-9, 2014
Visit our booth
Save money on
your transfection
reagents
Learn tips and tricks
from our product
specialists
Attend a demonstration
and have a chance to
win prices
Ingenio® Electroporation
Products
See last page for
information regarding
address and tickets
High efficiency electroporation using any
­electroporator
• High efficiency electroporation of hard to transfect cell lines and
­primary cells
• Compatible with most conventional electroporation devices including
Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
• Ideal for plasmid or siRNA electroporation
• Save money and reduce research costs while maximizing results
Ingenio® Solution Provides Comparable Efficiency on Amaxa®
­Nucleofector® Device
Liquid handling
Buy One, Get One
For Free*
CyBi®-SELMA
Semi-automatic 96- or
384-fold pipettor
* offer is valid to the end of June 2015, please write NB0415 on order
NEW!
Liquid handling
CyBi®-FeliX
Compact pipetting platform
for fully automated single- to
multichannel liquid handling
TransIT-X2®
Dynamic Delivery
­System
A novel, polymeric system for efficient delivery
of plasmid DNA and/or siRNA/miRNA
TransIT-X2® System Enables Superior Gene
­Expression v.s. Lipofectamine® 2000 in a Variety
of Cell Types
•
Efficient–Exceptional Broad ­Spectrum
DiagnosticsTransfection
and research
• Versatile–Cutting Edge ­Delivery of Plasmid
kits
DNA and siRNA
• ELISA
•
• Novel
PCR Technology-Non-­Liposomal, Polymeric
elivery
• ­D
NGS
kits
Research
reagents for
• Glycobiology,
• Cell biology
• Flow cytometry
Multilabelreader
Most of the news
will also be shown at
Lab14 in Norway, see
last page for more
information
Sense: Microplate reader
with touchscreen – it has
®
never been
easier
TransIT-X2
enables superior or similar Gene Expression in 26 out of 30 cell
types compared with Lipofectamine® 2000 using a plasmid DNA encoding luciferase. ­Luciferase expression was compared at 24 hours post-transfection using
a standard luciferase assay. Three different reagent-to-DNA ratios were used.
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
5
KAPA HYPER PLUS
NGS library preparation. Evolved.
Bronwen Miller, M
Kapa hyperplus – a single-tube ngs library prep workflow
integrating enzymatic fragmentation
INTRODUCTION
Benefits include:
Continuous
improvements
to library
•
DNA fragmentation
and library
prep inpreparation
2.5 hours for
next-generation
sequencing
(NGS)
to achieve
•
flexible DNA sample
input from
1 ngare
- 1 necessary
ug
•
reduced
bias
andquality.
more uniform
the
highest
data
One of sequence
the crucialcoverage
steps within library
•
PCR-Free workflows
preparation
is the initial DNA fragmentation, which can be
H
SE
ERPLUS – A SINGLE-TUBE NGS LIBRARY P
INTEGRATING ENZYMATIC FRAGMEN
IN HIGH YIELDS AND LOW SE
accomplished through either mechanical or enzymatic
processes.
Mechanical
methods
for DNA fragmentation
The KAPA
HyperPlus
kit provides
a streamlined
are difficult to scale or automate, and require large investments
workflowinstrumentation.
that includes fragmentation
and
libraryfor
in expensive
Current enzymatic
solutions
preparation
in a typically
single tube.
Building
on industry-leading
DNA
fragmentation
exhibit
sequence
bias, provide
poor
control
over
fragment
length
distribution,
and are solution
highly
library construction efficiencies, this integrated
sensitive
to input
amount.fragmentation,
combines
enzymatic
similar in quality to
Single Tube
Fragmentation
10-45 min
30 min
End Repair and A-tailing
Adapter Ligation
Benefits include:
15 min
• DNA fragmentation and library prep in 2.5 hours
• flexible DNA
sample
input from 1 ng
1 ug
Bead
Cleanup
30-min
• reduced bias and more uniform sequence coverage
The
con
Figu
cons
The
inpu
DNA
KAP
for b
Kets, Beverly
Rooyen,
Heather
Whitehorn,
Martin• Ranik,
Piet
Jones, Adriana Geldart, R
with
the
and convenience
To mechanical
addressvan
theseshearing,
challenges,
we
havespeed
developed
the KAPA
PCR-Free
workflows
Library
Amplification
HyperPlus
Library Preparation
Kit by integrating an enzymatic
of tagmentation-based
workflows.
DNA fragmentation technology with fast and efficient library
construction to provide a streamlined, easy-to-automate,
single-tube solution for preparing NGS libraries from
1 ng – 1 µg of dsDNA.
(optional)
30 min
Bead Cleanup
30 min
For human exome libraries, the HyperPlus workflow show
Total
time:
enzymatic-fragmentation methods
and
leads to significant
~2.5 hourswith high-quality
dropout is similar for all three workflows
Nextera with the FFPE sample.
ELDS LEAD TO IMPROVED
SULTS
TUNABLE AND
nversion ofFRAGMENTATION
input DNA to adapter-ligated library. This higherIS
library
lex libraries and improved sequencing metrics, including lower duplicate rates.
REPRODUCIBLE
90
Reproducible library fragment size distributions are obtained from a variety of
DNA sample inputs.
80
70
AT-dropout
Covaris + Hyper Prep
10
Conversion Rate Ranges
8
(%)
40
20
10
0
60
2
50
40
0
30
GC-dropout
Figure 1: Fragmentation
profiles obtained with different DNA inputs. 12
100 ng
10 ng
Input Amount
1 ng
Conversion
rate, defined as % input DNA converted to sequenceable, adapterDuplication Rates
Figure 4. HyperPlus
exomeultimately
capture libraries
ligated library, is a key library construction
metric, which
determines
generate fewer duplicate reads than libraries
library
diversity and quality.
Covaris + Hyper
Prep
HyperPlus
produced using Covaris.
Roc
pre
imp
25
6
20
4
15
(%)
1 µg
Covaris + Hyper Prep
Various input amounts of E.coli gDNA, were processed using
the
HyperPlus
HyperPlus Kit10with fragmentation times of 15 or 30 minutes
at 37˚C.
Nextera
samples
After library amplification
and a single 1X SPRI® bead cleanup,
8
were analyzed using an Agilent High Sensitivity DNA Assay™.
(%)
10
0
FFPE
gDNA
20
2
6
50
30
Existing
solutions for NGS library construction are extremely sensitive to
90 commercial enzymatic fragmentation
HyperPlus
6
sample80type and input amount, but the KAPA
HyperPlus
Covaris
+ Hyper PrepKit provides reproducible results across a range of inputs.
Covaris
Nano Prep
4
Library70insert sizes can be adjusted by varying
the+ Illumina
fragmentation
time.
% Conversion
version of
-sheared
it or the
yperPlus
100%
Achieve
conversion rate
Nextera
100
ibrary
HyperPlus
Duplicates (%)
60
12
A. Libraries were prepared from 200 ng of high
molecular weight FFPE DNA (403s, 414s) or
0
FFPE
DNA
(204s, 214s)
and captured
Kem-En-Tec Nordic ·degraded
Finland
+358
9594822
· Sweden/Norway
+46 18 149000 ·gDNA
Denmark +45 3927 1777
FFPE
with the Roche Nimblegen™ double-capture
protocol (Data courtesy of Dr. Brian Walker
10
5
0
DNA Analyzer
Analyze 48
96-well plates
in one day!
-The easiest
DNA fragment
analysis ever.
Place your PCR plate directly
from the thermocycler into the
ZAG for a complete analysis in 20
minutes. Automated data output
which can easily be integrated
into a LIMS.
ZAG
Zero Agarose Gel
DNA Analyzer
For fewer samples or RNA,
we offer The Fragment Analyzer.
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
7
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OB bsh rde start ic.c
e o d rd
W sy ser an c-no
ea u te
r ate nfo cre -e
–
m
ke
w.
w
w
t
ou
it
y!
as
–e
New
revolutionary
Centrifuge
Meet us!
Meet the Worlds first combined
­centrifuge/vortexer for PCR plates
Exhibitions/­
Conferences in
Nordic 2015
The patented Spin–Mix–Spin technology is intended to spin-down micro
volumes of reagents on the well’s bottom (first centrifugation spin), following
mixing (mix) and spin-down the reagents again from the walls and cap of the
well (second spin). Ideal for sample prep.
Denmark
April 25-28
ECCMID the 25th European
­Congress of Clinical Microbiology
and Infectious Diseases in Bella
Centre
May 20
Rigshospitalets Symposia
– At Rigshospitalet
Patented
technology
May 27-29
DSKB – Danish society of Clinical
Biochemistry
Sep 23-24
LabDays – Aarhus, Scandinavian
Congress Center at Radisson Blu
Sweden
May 5-6
SLAS (Society of Laboratory Auto­
mation & Screening) & Nordic
Biology Meeting, Karolinska Solna
May 6-7
Clinical Immunology Spring
­Meeting, Lund
Campaign price
9.175,- DKK
11.750,- SEK
11.050,- NOK
1.234,- €
(incl rotor for 2 PCR plates)
PS: The front page shows the endangered
blue-footed Sule from the Galapagos Islands,
sponsored of our supplier KAPA Biosystems.
8
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
June 16-17
ELRIG (European Laboratory &
Research Innovation Group),
Astra Zeneca Mölndal
November
NOV2K (Karolinska)
Finland
March 19
Biocenter Day Oulu
March 26-27
Virology Days, Kuopio!
Visit us and win nice prizes
and knowledge!