Laser Ablation Tomography New discoveries begin with LAT™ 1 2 3 Laser Ablation Tomography (LAT™) is capable of rapidly acquiring structural and compositional data in three dimensions with micron resolution. LAT™ uses fluorescent and hyperspectral imaging to obtain highly contrasted, colorful stacks of images in opaque biological specimens. An ultraviolet laser-sheet is created with a 355 nanometer q-switched, pulsed laser and a rapidly-swept galvanometer scanner. A camera focused on the face of the laser-sheet cap- 4 5 tures images of the sample as it is being incrementally pushed into the sheet from the opposite side. Due to the Gaussian distribution of the laser-sheet radiation, the surface layer of the sample that is being pushed into the laser-sheet is auto-fluoresced just prior to ablation. This process is continuously repeated until the desired sample volume is achieved. The high intensity of the laser-sheet induces fluorescent emission in the sample’s surface layer, producing distinct spectral characteristics depending on molecular composition. After imaging a complete sample, the stack of images is processed and reconstructed into LAT™ Process - (1) Cross-sectional images produced of Maize root. (2) Successive images are stacked together. (3) A 3D rendering of the sample is created. (4 & 5) 3D rendering can be segmented to quantify features of interest. a high-resolution volume rendering that can be viewed, manipulated and virtually dissected. Depending on the sample material and its size, images can be acquired at a rate greater than ten per second. This method allows for data acquisition at unprecedented speed without the need for staining or complex preparatory procedures. LAT™ is ideally suited for life science applications such as high-throughput phenotyping, general anatomical inquiries, and scientific discovery. Featuring: • Geometric and Microanatomic Structure Visualization • Compositional Visualization • Morphometric Analysis • Hyperspectral Imaging • Fluorescent Microscopy • Micron Resolution • Tomographic Models & Animations Lasers for Innovative Solutions llc 200 Innovation Blvd. Suite 214 State College, PA 16803 T: (814) 531-5447 E: brain.reinhardt@l4is.com W: www.l4is.com