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Store at
-20ºC
#15102
cGAS (D1D3G) Rabbit mAb
www.cellsignal.com
Support: 877-678-TECH (8324)
www.cellsignal.com/support
100 µl (10 western blots)
Orders: 877-616-CELL (2355)
orders@cellsignal.com
New 04/15
Entrez-Gene ID #115004
UniProt ID #Q8N884
For Research Use Only. Not For Use In Diagnostic Procedures.
Applications
W
Endogenous
Species Cross-Reactivity*
Molecular Wt.
Isotype
H
62 kDa
Rabbit IgG**
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
4
M 60
CF
7
TH
P1
U93
7
2
-9
NC
Recommended Antibody Dilutions:
Western blotting
200
140
1:1000
For product specific protocols and a complete listing
of recommended companion products please see the
product web page at www.cellsignal.com
100
80
cGAS
60
50
40
30
Specificity/Sensitivity: cGAS (D1D3G) Rabbit mAb recognizes endogenous levels of total cGAS protein.
Source/Purification: Monoclonal antibody is produced by
immunizing animals with a synthetic peptide corresponding to
residues surrounding Ala19 of human cGAS protein.
NK
kDa
**Anti-rabbit secondary antibodies must be used to
detect this antibody.
I-H
Background: Cyclic GMP-AMP synthase (cGAS, MB21D1) is
an antiviral enzyme that produces the second messenger cyclicGMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The
cGAS protein acts as a cytosolic DNA sensor that binds DNA
and produces the cGAMP second messenger from ATP and GTP
(1,2). cGAMP binds to and activates STING, a transmembrane
adaptor protein that is a critical component of the cellular innate
immune response to pathogenic cytoplasmic DNA (1-4). STING
is ubiquitously expressed and found predominantly in the ER
(3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and
activates interferon regulatory factors (IRFs) and NF-κB, which
leads to the induction of type I interferon and other immune
response genes (3-5).
20
Western blot analysis of extracts from various cell lines using cGAS
(D1D3G) Rabbit mAb.
Background References:
(1) S un, L. et al. (2013) Science 339, 786-91.
kDa
200
(2) W
u, J. et al. (2013) Science 339, 826-30.
140
(3) Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
100
80
(4) Z hong, B. et al. (2008) Immunity 29, 538-50.
(5) Ishikawa, H. et al. (2009) Nature 461, 788-92.
cGAS
(Myc-tagged)
60
50
40
30
20
–
+
hcGAS-Myc
Western blot analysis of extracts from 293T cells, mock transfected (-) or
transfected with a construct expressing Myc-tagged full-length human
cGAS protein (hcGAS-Myc; +), using cGAS (D1D3G) Rabbit mAb.
Tween is a registered trademark of ICI Americas, Inc.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS,
0.1% Tween®20 at 4°C with gentle shaking, overnight.
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©2015 Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Applications: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide Species Cross-Reactivity: H—human M—mouse R—rat Hm—hamster
Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology.