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Keys to Real-­‐time PCR Success! Technical Skills Seminar Series When: Tuesday, May 26th, 2015 9:30 AM – 3:30 PM Where: University of Pennsylvania BRB II/III Lobby and Auditorium O you Speakers: 9:30 -­‐ 10:30 10:30 -­‐ 10:45 10:45 -­‐ 11:20 11:20 -­‐ 12:00 12:00 -­‐ 12:30 12:30 -­‐ 1:00 1:00 -­‐ 1:30 1:30 -­‐ 1:45 1:45 -­‐ 2:15 2:30 -­‐ 3:30 Marcia Slater, Scott Gardner, Geoff Jackson Introduction to Real–time PCR Basics of Quantitation Review of TaqMan and SYBR Green Chemistries Analysis Methods (Standard Curve and Relative Quant) Break and Additional Registration Assay Design for Real–time PCR TaqMan Assays, Arrays, Cards, and Plates SYBR Assay Design Troubleshooting/Tips and Tricks Pizza Lunch Digital PCR Digital PCR QuantStudio™ 3D AnalysisSuite™ Break StepOnePlus /Viia7/QuantStudio 6, 7, 12k-­‐flex Software Overview qPCR Data Analysis: Thermo Fisher Cloud™ The seminar is FREE, but please PRE-­‐REGISTER, so there is enough PIZZA for everyone! Register at http://lifetechnologies.com/eventregistration.html If you would like an individual Consultation with an expert please inquire for appointment times. For questions please email: Heather.Stark@ThermoFisher.com