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Ekspot™ nanoLC-MALdI spotter
System Specifications
Coupling NanoLC to MALdI mass spectrometry results in an extremely powerful
tool for the analysis of complex peptide and/or protein samples in proteomics.
Use the Ekspot™ nanoLC-MALdI spotter in combination with our ekspert™
nanoLC 400 and ekspert cHiPLC® systems for a flexible and reproducible
nanoLC-MALdI system.
Single point control
One software program controls both the NanoLC and the Ekspot,
providing simple setup of methods and sample batches. A UV
signal from any UV detector with analog output can be used to
monitor the separation using an optional Ad converter.
High resolution spotting
Spotting can be done with a frequency of max. 1 spot every four
seconds in order to optimize resolution. In combination with the
ekspert™ nanoLC 400 and cHiPLC’s separating power you will
have the most advanced LC-MALdI system available.
Maximize throughout
The Ekspot holds up to 8 micro-titer plate sized targets, for use
with the AB SCIEX TOF/TOF™ 5800 System and the 4800 Plus
MALdI TOF/TOF™ Analyzer. Adapters for other MALdI mass
spectrometers are available as well.
Flexible design
In addition to spotting on a variety of MALdI MS targets,
the Ekspot can also be used as a micro-fraction collector for
applications such as off-line multi-dimensional separations.
A unique routine is used to “touch-off” droplets against the
wall of a collection vial or well.
Built-in matrix delivery pump
The Ekspot is designed to deposit fractions eluting from a
NanoLC column onto a MALdI target, and the MALdI matrix is
automatically mixed-in post column.
SYSTEM SPECIFICATIONS
dimensions
21 x 15 x 25.5” (53 x 38 x 65 cm) (W x d x H)
Weight
35 pound (16 kg)
Positioning Precision
100 μm
Spotting Frequency
user adjustable in 1 second steps; 4 second minimum
Flow rate range
200 nL/min to 4 μL/min for MALdI spotting
Max. 50 μL/min for fraction collection
Matrix Pump
Syringe pump. 50 uL syringe standard. 100 and 250 uL volume optional
SYSTEM SPECIFICATIONS (continued)
Matrix pump flow rate range
400 nL/min to 5 μL/min with 50 μL syringe
Plate CapacityDepending on configuration up to 8 microtiter plate format targets or up to
16 AB SCIEX 4700 targets
Wetted Parts
Fused silica, Fluoropolymer and PEEK for nanoLC flow path
glass, fused silica, fluoropolymer and PEEK for matrix flow path
Instrument Control
Eksigent control software
Ordering Information
950-00091
Ekspot MALDI spotter with adapters for 4 AB SCIEX 4800/5800 MALDI targets included.
Includes matrix delivery pump and control software. Installation and training included if
ordered as part of a nanoLC system; does not include a computer.
950-00040
Ekspot MALDI spotter. Capacity of four microtiter plate format targets, or 8 AB SCIEX
4700/Waters targets. Adapters for MALDI plates (4 required) are not included.
Includes matrix delivery pump and control software. Installation and training included
if ordered as part of a nanoLC system; does not include a computer.
950-00047
Ekspot MALDI spotter. Capacity of eight microtiter plate format targets, or 16 AB SCIEX
4700/Waters targets. Adapters for MALDI plates (8 required) are not included.
Includes matrix delivery pump and control software. Installation and training included
if ordered as part of a nanoLC system; does not include a computer.
620-00134
Adapter for two AB SCIEX 4700/Voyager targets
800-00250
Adapter for one AB SCIEX 4800/5800 target
620-00136
Adapter for one Bruker Anchorchip 384 MT target
620-00119
Adapter for two Waters 96/384 targets
500-00496
Adapter for Thermo MALDI targets
5019951
Analog to digital converter for Ekspert nanoLC 400 system
For Research Use Only. Not for use in diagnostic procedures.
©2013 Eksigent Technologies. All rights reserved. Information subject to change without notice. Eksigent is a registered trademark of Eksigent Technologies. NanoLC, CHiPL, and MicroFlow
are trademarks of Eksigent Technologies. All other trademarks are property of their respective owners. Patents pending worldwide.
6960313-01 01/2013
Headquarters
5875 Arnold Road | Dublin, California 94568
Phone 925-560-2600 | Fax 925-560-2700
www.eksigent.com