INTERNATIONAL Solutions for Mine Site Rehabilitation and Tree Species Selection Solutions for soil, plant & environmental monitoring 17/11/2011 INTERNATIONAL www.ictinternational.com 1 Stawell Gold Mine - Location: western Victoria, Australia. - Gold mining in Stawell extends back over 120 years. - Certain areas near the mine will be rehabilitated with vegetation. - Three tree species were examined for their tolerance to the hot, dry environment using HRM Sap Flow Meter (SFM1). Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 2 HRM Sap Flow Meter – SFM1 - Measures sap flow (water movement) or transpiration in woody plants - Data can be used to accurately measure total plant water use on a hourly or daily basis - SFM1 can measure stems as narrow as 1cm as well as the largest trees on earth Solutions for soil, plant & environmental monitoring 17/11/2011 INTERNATIONAL www.ictinternational.com 3 Sap Flow and Stawell Gold Mine - SFM1 instruments were installed on nine trees from three native tree species - Sap flow data were collected over 18 months and correlated with temperature and vapour pressure deficit (VPD) - Temperature was a measure of hotness - VPD was a measure of dryness - Sap flow response to increased hotness and dryness was used as a measure of tolerance Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 4 Tree Species at Stawell Gold Mine Eucalyptus cladocalyx Sugar Gum Eucalyptus melliodora Yellow Box Eucalyptus polybractea Blue-leaved Mallee Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 5 Installation of Equipment Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 6 Measuring Correction Factors Stem cores and methyl orange for sapwood depth estimation Cut tree stems for comprehensive measurement of bark, sapwood and heartwood depth HRM probes were installed here. Wound response can be measured under a microscope. Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 7 Rainf 40 - J (cm3 cm-2 day-1) is sap flux density corrected for stem diameter. - Eucalyptus cladocalyx has up to 4 times the amount of sap flux than the other species. - Sap flux showed a strong seasonal pattern: lower in winter, higher in summer. 0 100 80 -2 - Sap flow was recorded over an 18 month period. Gaps in the data caused by solar panel malfunction. 20 60 3 -1 3 -2 -1 3 -2 -1 J (cm cm day ) J (cm cm day ) J (cm cm day ) 18 Months of Sap Flow Monitoring 40 (D) – E. cladocalyx 20 0 100 80 (E) – E. melliodora 60 40 20 0 100 80 (F) – E. polybractea 60 40 20 0 Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar 2009 2010 2011 Data and figure by Michael Forster, ICT International Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 8 Temperature and Sap Flow Sap flow for E. cladocalyx and air temp on 21/12/09ca 24lper day 2000 45 40 1500 Sap flow cm3 30 1000 25 20 500 15 Air temperature oc 35 MILD DAY: Max. temperature = 28 C Transpiration = 24L Species: E. cladocalyx sap volume Temp 10 0 5 -500 0 Data & Graph: Augustine Doronila, University of Melbourne Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 9 Temperature and Sap Flow Sap flow for E. cladocalyx and air temp on 23/12/09 ca 20l per day 2000 1500 45 WARM DAY: 40 Max. temperature = 36 C Transpiration = 20L Species: E. cladocalyx Sap flow cm3 30 1000 25 20 500 15 Air temperature oc 35 sap volume Temp 10 0 5 -500 0 Data & Graph: Augustine Doronila, University of Melbourne Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 10 Temperature and Sap Flow 1800 45 1600 40 1400 35 1200 30 1000 25 800 20 600 15 400 10 200 5 0 0 HOT DAY: Air temperature oc Sap flow cm3 Sap flow for E. cladocalyx and air temp on 10/01/10 ca 17l per day Max. temperature = 41 C Transpiration = 17L Species: E. cladocalyx sap volume Temp Note: transpiration declines once temperature reaches approx. 30 C. Tree is still transpiring in order to not over heat and cause damage to leaves and photosynthetic systems. Data & Graph: Augustine Doronila, University of Melbourne Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 11 Species Tolerance to Temperature Temperature The optimal range of temperature ( C) for maximum sap velocity (Vs, cm hr-1) in each stem measured in this study. Results indicate Eucalyptus cladocalyx can tolerate hotter weather. Optimal temperature range: Eucalyptus cladocalyx: 21 C to 30 C Eucalyptus melliodora: 20 C to 27 C Eucalyptus polybractea: 16 C to 26 C 40 Temperature (°C) There was a significant difference following one-way ANOVA (F = 17.321, d.f. = 8, p < 0.001) and the results of Tukey’s HSD post-hoc test are indicated by letters above each box plot. 45 a a a b ab b X2 X5 X7 EC EC EC 5 7 9 EM EM EM ab c a 35 30 25 20 15 10 E. cladocalyx E. melliodora 3 5 8 EP EP EP E. polybractea Data and figure by Michael Forster, ICT International Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 12 Species Tolerance to Dryness Vapour Pressure Deficit 7 6 a a ab c c c bc d ab There was a significant difference following one-way ANOVA (F = 15.232, d.f. = 8, p < 0.001) and the results of Tukey’s HSD post-hoc test are indicated by letters above each box plot. 5 VPD (kPa) The optimal range of VPD (kPa) for maximum sap velocity (Vs, cm hr-1) in each stem measured in this study. 4 3 Results indicate Eucalyptus cladocalyx can tolerate drier weather. 2 1 0 X2 X5 X7 EC EC EC 5 7 9 EM EM EM E. cladocalyx E. melliodora 3 5 8 EP EP EP E. polybractea Optimal VPD range: Eucalyptus cladocalyx: 1.5kPa to 3.2kPa Eucalyptus melliodora: 1.1kPa to 2.5kPa Eucalyptus polybractea: 1.0kPa to 2.7kPa Data and figure by Michael Forster, ICT International Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 13 Total Tree Water Use Summary of average daily water use (Q, L day-1) throughout the various seasons of the study period. Values are total tree water use including multiple stems of E. melliodora and E. polybractea. Values are litres of water per day (±SD). Species E. cladocalyx E. melliodora E. polybractea Summer ‘10 Autumn ‘10 Winter ‘10 Spring ‘10 Summer ‘11 26.35 (±8.49) 4.63 (±2.53) 7.46 (±7.82) 17.02 (±6.59) 2.67 (±1.43) 4.76 (±5.01) 11.21 (±4.29) 2.12 (±1.56) 3.62 (±3.52) 17.25 (±4.33) 4.59 (±2.52) 8.16 (±7.57) 26.59 (±4.18) 9.21 (±4.60) 4.97 (±1.36) Data and table by Michael Forster, ICT International Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 14 Conclusion Eucalyptus cladocalyx Sugar Gum - The SFM1 Sap Flow Instrument measured sap flow in nine trees over an 18 month period. - Eucalyptus cladocalyx showed the highest amount of water use, up to 4 times as much as the other species. - E. cladocalyx had greater tolerance to hotter and drier weather. - Successful rehabilitation at Stawell Gold Mine should involve E. cladocalyx. Solutions for soil, plant & environmental monitoring 17/11/2011 www.ictinternational.com 15 ICT International Pty Ltd Solutions for soil, plant and environmental monitoring www.ictinternational.com sales@international.com.au Phone: 61 2 6772 6770 Fax: 61 2 6772 7616 PO Box 503, Armidale, NSW, Australia, 2350 INTERNATIONAL Solutions for soil, plant & environmental monitoring 17/11/2011 INTERNATIONAL www.ictinternational.com 16
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