Tapan Chandra Mondal A Concise Text Book of Micro Biology Reading excerpt A Concise Text Book of Micro Biology of Tapan Chandra Mondal Publisher: IBPP http://www.narayana-verlag.com/b6037 Copying excerpts is not permitted. Narayana Verlag GmbH Blumenplatz 2 D-79400 Kandern, Germany Tel. +49 7626 9749 700 Fax +49 7626 9749 709 Email info@narayana-verlag.com http://www.narayana-verlag.com In our online-bookshop we present all english homeopathy books. Neisseria Group 56 Neisseria Group Classification: Neisseria Group Non-Pathogenic Pathogenic N.Catarrhalis N. Gonorrhoe N.Meningitidis Morphology: Cocci Arrangement: Arranged in pairs with apposed concave surfaces. Staining: Gm (-) Motility: Non-motile, no flagella, non-capsulated, nonsporing. Gonococci in urethral discharge (pus). Inset-enlarged view showing arrangement in pairs and adjacent sides concave. *** A Concise Text Book of Microbiology A Narayana Verlag; 79400 Kandern Tel: 0049 7626 9749700 Except from Dr. Tapan Chandra Mondal: A Concise Text Book of Micro Biology Neisseria Group ________________________________________ 57 Lesions: I. Primary: a) In adult male: Ant. Urethritis with pus formation preceeded by catarrhal inflammation - Gonorrhoea. b) In adult female: Urethritis & Endocervicitis. c) In male child: Urethritis. d) In female child: Vulvovaginitis due to presence of columnar epithelium in the vaginal part of cervis But in adults it is replaced by stratified Sq. epithelium. e) In newborn: Purulent conjunctivitis. II. Secondary: a) In adult male: Urethral stricture, chr. Prostatitis. Epididymitis, (Cystitis, Pyelitis, Pyelonephritis etc.) by lymphatic spread. b) In adult female: Bertholinitis, Bertholin abscess, Endometriosis, Salpingitis (causes sterility), Oophoritis, Pyosalpin, Tubo-ovarrian absess, Peritonitis, (Cystitis, Pyelitis, Pyelonehritis etc. c) Common to both ages: Arthritis, Iritis, Conjunctivitis, Acute Ulcerative Endocarditis. Lab. Diagnosis: A. Collection of Material: I. Acute: a) Male: Urethral discharge. b) Female: Urethral discharge or Cervical swab. II. Chronic: a) Male: Discharge from the seminal vesicle after prosthetic message. b) Female: Cervical Swab in the Estrogenic period of menstrual cycle or discharge after milking of the urethra or purulent discharge from the site of any other lesion. *** A Concise Text Book of Microbiology *** Narayana Verlag; 79400 Kandern Tel: 0049 7626 9749700 Except from Dr. Tapan Chandra Mondal: A Concise Text Book of Micro Biology 58__________________________________________ Neisseria Group B. Smear & Gm Stain: Gm (-); Bean shaped; Diplococcus with concave surfaces apposed; intracellular in pus cells or extra cellular. C. Culture: 1. Serum Agar: Greyish white, translucent, round & convex colonies. 2. Serum Broth: Broth becomes little turbid. 3. Chocolate Agar: Small pinhead colonies in Chocolate agar media. (1 % Tetraemethyl Para Phenylene Diamine Dihydrochloride is added over the colonies. Gonococci colonies turn pink immediately. It is called Oxides Test. Smear & Gm stain: Characteristic but not intracellular. Motility from serum broth - Non-motile. D. Subculture for pure form in Chocolate Agar media: Precaution for culture: i) Pus or discharge should be cultured immediately after collection as the organisms are very sensitive. ii) Culture should be done in 5-10% CO2 tension.) E. Biochemical: Ferments Glucose but not maltose with production of acid only. F. Serological: Type of the organisms can be identified by agglutination reaction using type specific antisera. For Indirect Diagnosis: Following tests are done in addition to above: Complement Fixation Test: Complement fixing antibodies appear in blood after 2 wks. of Gonococcal infection. These are diagnosed by Complement Fixation Test. Two Glass Tube Test: In Chr. gonorrhoea, the first urine of the patient collected in a clean glass test tube, if kept standing for sometime, shows formation of filamentous structure known as Prostatic threads. These threads are composed of Fibrin, Leucocyte & epithelial cells. A A Concise Text Book of Microbiology A Narayana Verlag; 79400 Kandern Tel: 0049 7626 9749700 Except from Dr. Tapan Chandra Mondal: A Concise Text Book of Micro Biology Neisseria Group 59 13.2 Meningococcus Morphology: Cocci Arrangement: Arranged in pairs with appeased surfaces flat. Staining: Gm (-) Fig. N. meningities in CSF- Inset enlarged view of the organism (showing adjacent sides flattened. Motility: Non-motile, no flagella, non-capsulated, nonsporing Lesions: i) Respiratory tract: Nasopharungeal catarrh. ii) General: Bacterium & Septicemia, Miliary abcess. iii) Bones: Acute Arthritis. iv) Head: Acute Leptomeningitis, Meningo-cephalitis, Brain abcess . v)Heart: Endocarditis. Lab. Diagnoses: Material: Blood, CSF, Nasal discharge. *** A Concise Text Book of Microbiology *** Narayana Verlag; 79400 Kandern Tel: 0049 7626 9749700 Except from Dr. Tapan Chandra Mondal: A Concise Text Book of Micro Biology 60 _____________________________________ Neisseria Group a) Smear & Gm. Stain in case of Nasal discharge: Gm (-) Cocci arranged in pairs with apposed surfaces flat. b) Culture: Serum broth - slightly turbid. Chocolate Agar - Small, transparent, convex glistening colonies (which are oxidase positive). Motility from serum broth: Non-motile. Gm Stain: Characteristic. Sub-culture to isolate pure from in chocolate Agar. (Precaution for culture) i) Incubated under 5-10% COa tension. ii) If treatment was already started, PABA Penicllinase to be added to media. Staining for morphology. c) Biochemical: Both glucose & Maltose are fermented with the production of acid only. d) Serological Reaction: The organisms can be identified by agglutination reaction using poly-valent antisera. (If blood is collected: Culture done as usual followed by above procedure). (If CSFis collected: i. Properties of CSF is studied, ii. Centrifuged deposit is cultured in the same way). *For CSF Chart See Appendix. Blood Picture in Meningococcol Infection: Leucocytosis with relative neutrophilia polymorpho nuclear Leucocytosis. A A Concise Text Book of Microbiology *** Narayana Verlag; 79400 Kandern Tel: 0049 7626 9749700 Except from Dr. Tapan Chandra Mondal: A Concise Text Book of Micro Biology Tapan Chandra Mondal A Concise Text Book of Micro Biology 160 pages, pb publication 2005 More homeopathy books on www.narayana-verlag.com