1. business and industrial
2. agriculture and forestry
3. aquaculture

1. AQUAFISH INNOVATION LAB PROPOSAL
COVER SHEET
Title of Proposal:
Development of more efficient and environmentally sustainable aquaculture systems for Nepal.
Names, institutional affiliation, address, telephone, and email address of Lead US Principal
Investigator submitting this proposal:
Dr. James S. Diana, School of Natural Resources and Environment, University of Michigan, Ann Arbor,
MI 48109-­1041, USA, jimd@umich.edu
Name and affiliation of all US and HC co-­PIs:
Dr. Madhav Shrestha, Agriculture and Forestry University, Nepal
Total Federal
Funds Requested
Total Cost
$499,809
Proposed Project Period:
Start:
(27 months maximum, ending 30 September 2015)
1 September
2013
Required prior to award
Total US non-­
Federal Cost
Share
$100,000
to End: 30 August 2015
Approved
Pending
Not necessary
(please attach approval)
(please attach approval)
(please attach signed waiver)
Animal Use Approval
Human Subjects Approval
X
X
1,&5$/HDG,QVWLWXWLRQ·V1HJRWLDWHG,QGLrect Cost Rate Agreement)
x
Institutional and Agency Certifications and Assurances
x
Development Theme
A Improved Health and Nutrition, Food
Quality, and Food Safety
B Income Generation for Small-­Scale Fish
Farmers
Region
Central America
South America
Caribbean
Eastern Europe
Africa
% effort:
% effort:
% effort:
% effort:
% effort:
Authorized US Institutional Representative
(Typed name and telephone number)
X
C Environmental Management for
Sustainable Aquatic Resources Use
D Enhanced Trade and Investment for
Global Fishery Markets
Southeast Asia
South Asia
Central Asia
East Asia
Signature
% effort:
100% effort:
% effort:
% effort:
Date
AQUAFISH INNOVATION LAB
PROPOSAL SUMMARY PAGE FORM
Title of Proposal:
Development of more efficient and environmentally sustainable aquaculture systems for Nepal.
Development Theme (select only one)
A Improved Health and Nutrition, Food
C Environmental Management for
X
Quality, and Food Safety
Sustainable Aquatic Resources Use
B Income Generation for Small-­Scale Fish
D Enhanced Trade and Investment for
Farmers
Global Fishery Markets
Names and institutional affiliations of Principal Investigator(s) and Co-­PIs submitting this proposal:
Dr. James S. Diana, School of Natural Resources and Environment, University of Michigan, Ann Arbor,
MI 48109-­1041, USA
2.
Title of
Investigation
1. Reproduction and seed
production of sahar ( Tor
putitora) in Chitwan, Nepal
2. Production of periphyton to
enhance yield in polyculture
ponds with carps and small
indigenous species
3.
Household fish ponds in
Nepal: their impact on fish
consumption and health of
women and children; their
constraints determined by
value chain analysis; and their
extension through school
SRQGVDQGZRPHQ¶VILVK
farming groups
4. Introduction of two small
indigenous species to improve
sustainability in typical
polyculture systems in Nepal
5. Using standard methods to
evaluate experimental seed
production and culture
techniques for Singhi
( Heteropneustes fossilis, Bloch)
in Nepal
Topic Area
(RFP p. 3-­4)
Quality
seedstock
development
Sustainable feed
technology and
nutrient input
systems
TYPE OF
INVESTIGATION
EXPERIMENT/
STUDY/ ACTIVITY
Total
Cost 1
(US $)
Host
Country
Institutions
Involved
Study
$73,500
AFU
Experiment
$76,500
AFU
Human nutrition
and human
Study
health impacts of
aquaculture
$121,104
AFU
Production
system design
and best
management
alternatives
Experiment
$128,705
AFU
Activity
$50,000
AFU
Quality
seedstock
development
Diana; Environmentally sustainable aquaculture systems for Nepal Page 1 6. Demonstrating the value of
tilapia and sahar production in
polyculture ponds using
government farm and on-farm
trials
Production
System Design
and Best
Management
Alternatives
Study
$50,000
Directory of
Fisheries
Development
Sum: $499,809
1
The sum of the costs of all investigations should equal the total cost of federal funds requested on the cover sheet.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 2 3. PR O POSA L N A R R A T I V E
E X E C U T I V E SU M M A R Y A N D I N T R O D U C T I O N
Nepal is a poor country; most residents are at best educated at the level of primary schooling, and
many are undernourished or even malnourished (Bhujel et al. 2008). As a result of this poverty,
most planning documents produced by the government, as well as outside organizations,
concentrate on human health and nutrition as the main focus for future development of
aquaculture. This focus is long standing. In 1976, Rana and Rajbanshi developed a National Plan
for Development of Aquaculture for Nepal, which focused on increasing production of household
ponds and other systems that would provide nutrition to poor households as the main goal.
Subsequent plans in Nepal, including the F isheries Perspective Plan (GoN 2000), the Strategic
Vision of Agriculture Research (NARC 2010), and evaluations by FAO (2013) all maintain
nutrition for poor families as the main focus.
Throughout the poorer countries of Asia, small indigenous species of fish (SIS) are promoted as
a means to provide health benefits for poor consumers. The benefits of their consumption include
increased intake of calcium and vitamins (such as vitamin A) because the fish are generally
consumed whole. In addition, these fish, when cultured or captured, are generally consumed in
the home rather than sent to market, so they provide direct nutrition. While SIS can be caught
from natural waters, they have not been well incorporated into aquaculture production systems.
For example, the carp polyculture systems, which are the mainstay of commercial aquaculture in
Nepal, largely use 5-7 carp species, all targeted on large carp sold to market. While SIS could be
incorporated into these polyculture systems ² possibly without any loss of production for the
large carp species ² this has seldom been done and there is no research basis to indicate whether
such incorporation would be helpful or damaging to overall production. Over the first two years
of this grant, our project will focus on incorporation of SIS into polyculture systems to determine
if it is a viable means to increase food production for poorer households.
Since the mid-1980s aquaculture development in Nepal has focused on utilizing marginal
agricultural lands, such as gholes (flooded areas with marginal agriculture potential), to serve as
aquaculture sites for poor households. As a result of outreach conducted in the Terai (the low
elevation plains area of southern Nepal), numerous household ponds have been built in these
marginal agricultural areas, and the management of those ponds with cages has been promoted as
a means to substantially improve nutrition of poor households. Such outreach was initiated by
our CRSP colleagues and the government of Nepal to extend aquaculture into gholes, and it has
been promoted in all of the aquaculture plans for Nepal, yet the success of these systems and
their effects on household nutrition remain uncertain. This is the second main focus of our
proposal: to evaluate the success of household ponds in increasing fish consumption by women
and children in poor households, and to then determine if this increased consumption leads to
improvements in health, as measured by World Health Organization standards of nutritional
status for children. While we began work in this area during the last project phase, it was
exploratory work and will aid in the design of this new proposal.
Our first goal: to evaluate SIS and their role in household nutrition, and second goal: to evaluate
use of gholes in aquaculture production, are emphasized in investigations #2, 3, and 4.
,QYHVWLJDWLRQLVHQWLWOHG³Production of periphyton to enhance yield in polyculture ponds with
Diana; Environmentally sustainable aquaculture systems for Nepal Page 3 FDUSVDQGVPDOOLQGLJHQRXVVSHFLHV´,WIRFXVHVRQWZRPDLQREMHFWLYHVWKHILUVWLVWRLQFOXGH6,6
in polyculture systems to enhance household consumption and nutrition, and the second is to
provide substrates to stimulate periphyton production and increase the yield of polyculture
ponds. Both of these objectives should result in improved pond efficiency as well as better
economic and health conditions for pond owners.
Investigation #3 is a cooperative project between University of Michigan (UM), Agriculture and
Forestry University of Nepal (AFU), Winrock International, and the Nutrition CRSP. It is
HQWLWOHG³Household fish ponds in Nepal: their impact on fish consumption and health of women
and children; their constraints determined by value chain analysis; and their extension through
VFKRRO SRQGV DQG ZRPHQ¶V ILVK IDUPLQJ JURXSV´ ,W ZLOO FRQVLVW RI VXUYH\V IURP IRXU DUHDV RI
Nepal investigating the added consumption of fish that occurs in a household from ownership of
household ponds. It has been designed around earlier work in Nepal focused on the same topic in
the area around Kathar. This investigation will also complete a value chain analysis of household
ponds to determine the constraints to their adoption, and use school pondV DQG ZRPHQ¶V ILVK
farming groups to extend the adoption of household ponds to more end users. This study should
help government intervention with household pond systems and benefit the small farmholders
using those systems.
Investigation #4 is again focused on including SIS in polyculture ponds with carps. It is entitled
³,QWURGXFWLRQ RI WZR VPDOO LQGLJHQRXV VSHFLHV to improve sustainability in typical polyculture
V\VWHPVLQ1HSDO´,WZLOODGGWRZRUNLQ,QYHVWLJDWLRQE\HYDOXDWLQJGHQVLWLHVRI6,6Xsed in
polyculture to maximize yield and family nutrition. These alterations will enhance pond
efficiency and most likely economic performance of ponds for the larger scale polyculture ponds.
A third area of focus for our proposed research is the enhanced production of native species,
particularly sahar, a cool water species indigenous to Nepal. Again, all of the aquaculture
planning documents described above had a focus on fish production in colder regions of the
country. While these plans generally called for trout culture, we have maintained that sahar may
be a more successful alternative because it is a native species, valued by local inhabitants, and
important as a target of restoration. We have conducted research on sahar production systems in
previous CRSP funding and have demonstrated their utility in polyculture systems. In this
proposal, we will incorporate sahar into polyculture systems using on-farm trials, as well as to
expand sahar seed production to other regions of the country. While we may begin to evaluate
expansion of trout culture in the country over the five-year funding timeline, this is not our
current goal. There is a nascent trout production near Pokhara, and we will evaluate its potential
and role in the future if we are successful in initiating the studies in this proposal. In addition to
sahar, we propose work to establish aquaculture systems for another indigenous species, the
stinging catfish. This is early development work, as culture of this species is in infancy, and our
project to produce a demonstration farm, induced spawning, fry rearing, and some on-farm trials
is the first in a series to promote this species. Over the five-year life of this project, we intend to
conduct further, more directed research on constraints of catfish production, based on the
outcome of our demonstration trials.
Our third goal is again the focus of three investigations: #1, 5, and 6. Investigation #1 is entitled
³5HSURGXFWLRQDQGVHHGSURGXFWLRQRIVDKDU Tor putitora ) in Chitwan, NepDO´,WLVFRRperative
Diana; Environmentally sustainable aquaculture systems for Nepal Page 4 between AFU and the Nepal Agricultural Research Council (NARC). Sahar is a locally
important species for wild fisheries and human consumption, and we have focused on various
aquaculture techniques for polyculture of this species in the past. This proposal focuses on
adapting the seed production methods currently being used by NARC in Pokhara and developing
another production center at AFU, Rampur. Once the methodologies for seed production and
fingerling rearing are completed, we believe fish for restocking and for aquaculture systems will
become more available throughout Nepal.
Investigation #5 focuses in the area of seed production and new aquaculture systems for native
ILVK,WLVHQWLWOHG³Using standard methods to evaluate experimental seed production and culture
techniques for Singhi ( Heteropneustes fossilis %ORFK LQ 1HSDO´ ,W ZLOO EHJLQ H[Sloring seed
production and grow-out systems for a locally important species.
Finally, Investigation #6 extends new aquaculture technology to local farmers. It is entitled
³'HPRQVWUDWLQJ WKH YDOXH RI WLODSLD DQG VDKDU SURGXFWLRQ LQ SRO\FXOWXUH SRQGV XVLQJ D
government farm and on-IDUPWULDOV´,WZLOOXVHJRYHUQPHQWIDFLOLWLHVDQGIDUPSRQGVWRSURGXFH
demonstration systems for polyculture systems at use in Nepal, as well as educate local farmers
about the benefits of adding new species on pond production.
Besides the linkages between national aquaculture plans and these investigations, this project is
also coordinated with the goals USAID and the Mission in Nepal. The Feed the Future (FtF)
program of USAID is focused on undernutrition, especially in women and children, and
increasing the availability and access to nutrient dense foods. Of relevance to aquaculture and
this proposal is the focus on small-scale farming and methods meant mainly to enhance
household consumption of fish rather than sale to market. While both local consumption and
market sales can enhance family nutrition, use of household ponds and their products is more
direct and controllable. For example, Bhujel et al. (2008) demonstrated that women in Nepal
from low-income households tend and manage farms, while the men often leave the household to
secure employment elsewhere. So fish products from household ponds will be available for
women to use directly in the household and should therefore directly improve nutrition.
This proposed research is of direct relevance to FtF research initiatives in several ways. The first
FtF research goal is to advance the productivity frontier by increasing productivity beyond
current levels through technology development, and extending technology so that local
production can reach the level of research farms. Our work on SIS and polyculture, stinging
catfish production, and sahar culture has the same intent: to first determine means of enhancing
yield by production of additional species in the polyculture ponds, then to evaluate the success of
farmers applying these systems to their own ponds.
A second component of this FtF goal is to increase the supply of livestock, including fish, to
prevent undernutrition in children. This is particularly important for smallholder farmers who
face the challenge of growing a crop to sell and produce some income with the constraint of
eating some of the crop to improve household nutrition, but at the same time, this consumption
reduces profit. Inclusion of SIS into aquaculture systems alleviates this strain, as it most likely
will produce fish for household consumption while not diminishing the productive capacity of
the ponds for growing the marketable crop. In fact, the addition of periphyton-based aquaculture
Diana; Environmentally sustainable aquaculture systems for Nepal Page 5 into these ponds should also enhance overall production and increase production of marketable
fish as well as SIS.
Among the seven areas of concern for the USAID mission in Nepal, Agriculture and Food
Security is the first and foremost. About 70% of the workforce in Nepal relies on agriculture for
their employment, \HW RQO\ RI WKH FRXQWU\¶V *'3 LV IURP DJULFXOWXUH. Nepal struggles to
produce enough food for its people, and low nutritional status is a major issue in the country.
One of the targets of the USAID mission is to help small-holder farms in conflict-stricken and
flood-prone areas improve their production and standard of living. The work we propose on
household ponds and small-scale aquaculture fits well within this development context.
This proposal represents a collaboratively defined series of projects with host country
counterparts in Nepal. The experiments listed were defined largely by host country scientists, in
consultation with their university and government colleagues. The priority of each experiment or
study is exemplified by the fact that of all possible studies to be conducted, each investigator
believed this was the most important one.
In addition to these currently planned activities, we intend to move in the future into a more
detailed analysis of the limits to aquaculture within Nepal. One important limit seems to be the
restriction of most aquaculture operations to summer months. Since culture in the Terai region is
mainly done in tropical or subtropical conditions, there should be good potential to expand
throughout the year. However, most operations are seasonal, mainly because of low precipitation
and resultant low water levels in ponds over winter. We will begin to investigate the possibility
of expansion of the growing season in this series of studies, with the intent of better evaluation of
the possibility through controlled experiments and on-farm trials in the next series of studies. We
also intend to continue our work on the nutritional aspect of fish culture, mainly by expanding to
other types of farm systems, as well as uses of fish by non-culturists. One final goal for future
work is to better evaluate the dimensions and potential environmental impacts of the growing
aquaculture industry around the production of rainbow trout.
In addition to our intentions to conduct research and outreach as part of this proposal, we will
also improve the laboratories, ponds, and facilities of AFU so they are well suited to continue
aquaculture research and development in the future. AFU (formerly Tribuhavan University) and
UM have collaborated on projects for over ten years, and the facilities and infrastructure there
are suitable for the successful completion of these proposed projects. Our proposal includes
restoration of lab and pond systems, to bring them up to standards necessary to continue quality
research on aquaculture in Nepal in the future. Our previous CRSP activities have included
training of the future leaders in aquaculture for Nepal; both Rai Sunila and Madhav Shrestha
were students working under the CRSP and are now in positions of leadership in the country.
With the development of infrastructure, new students, and leaders, we are confident that Nepal
and AFU will be in a position to be competitive for future grants. UM and the AquaFish
Incubation Lab will likely phase out activities in Nepal after this five-year project, but the
infrastructure left behind and the people who have been trained will place Nepal in good
standing for the future. Our development of research projects and student activities mirrors this
exit strategy in that all components of our work are planned for a five-year horizon and will be
successfully completed during that time.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 6 I N V EST I G A T I O NS
University of M ichigan
Nepal Project
Investigation 1
a. T itle: Reproduction and seed production of sahar ( Tor putitora ) in Chitwan Nepal
(Quality seedstock development/ study)
b. Principal Investigators:
Jay Dev Bista ± Fisheries Research Center, NARC, Pokhara, Nepal
Madhav K. Shrestha ± Agriculture and Forestry University (AFU), Nepal
James S. Diana ± University of Michigan, USA
c. O bjectives:
c.1.
To extend sahar breeding technology to Chitwan from work done in Pokhara;
c.2.
To develop protocols for sahar reproduction and mass scale seed production in Chitwan;
c.3.
To establish nursing and rearing management practices of sahar fry in Chitwan; and
c.4.
To make sahar fry available for culture and restocking.
d. Significance:
Sahar (Tor putitora ) is an economically important, high-value indigenous fish species in Nepal
(Rai et al., 1997). The price of sahar in the Nepalese market is almost double, compared to the
commonly cultivated carps and tilapia species. Sahar is still taken in capture fisheries in lakes
and rivers, and no commercial cultivation has begun in Nepal. This species is declining from its
natural habitat mainly due to urbanization, illegal encroachment, over-fishing, and ecological
alterations of physical, chemical, and biological conditions in the natural environment (Bista et
al., 2008).
Various national aquaculture plans for Nepal, most recently NARC (2010) and FAO (2013),
have included the development of cold water systems for aquaculture in upland areas as a
priority. In addition, seed production is also recognized as a major bottleneck to aquaculture
development in Nepal. The potential culture systems for cool water areas include development of
trout culture from imported fish, as well as the use of indigenous fish like sahar for aquaculture.
Culture of indigenous species is a high priority globally, as it reduces issues with invasive
species introductions, is in harmony with local cultural needs, and increases the possible options
for aquaculture production. This proposal seeks to produce large volumes of sahar seed, as well
as develop nursing and rearing techniques, so that sahar culture can be extended from
experimental farms to more commercial systems.
Attempts to culture and conserve this species have been initiated in Nepal with major efforts to
develop culture technology and propagate the species (Gurung et al., 2001; Joshi et al., 2002).
This has led to a better knowledge of spawning biology, ecology, and behavior of sahar, as well
as preliminary growth performance in captive conditions. Enhanced growth in tropical and
subtropical ponds, as well as the recent breeding success in hatcheries, has raised new hopes on
the prospects of sahar aquaculture in Nepal (Shrestha et al., 2005, 2007; Bista et al., 2001, 2007;
Rai, 2008). In addition to culture of fish to adult size for consumption, these new developments
Diana; Environmentally sustainable aquaculture systems for Nepal Page 7 can contribute to rearing individuals that can be stocked into natural waters to replenish
populations there. Due to its omnivorous and predatory feeding, sahar has also proved to be a
good candidate to co-culture with mixed-sex tilapia to control tilapia recruits in a pond and
provide better size at harvest and yield of tilapia (Shrestha et al., 2011). Inclusion of sahar in
polyculture of mixed-sex tilapia with carps has enhanced production in these ponds (Jaiswal,
2012).
Sahar is known to be an intermittent in spawning behavior. It can spawn in most months, except
January, under cultured conditions, but in natural waters, it spawns during the monsoon when
rivers and streams are at peak flow. Sahar typically migrate a long distance from large rivers to
streams for spawning. The Fisheries Research Center in Pokhara is the only locations where fry
are produced, and this production is still in limited quantity. Demand for sahar fry has increased
for restocking in rivers and lakes, as well as for aquaculture production. Lack of availability of
fish seed is a major bottleneck for commercial production and conservation. Sahar breeding has
been attempted in the Aquaculture Department, of AFU in Rampur, Chitwan and has been
successful in producing 250 fry during a practical class in 2010.
This study is intended to transfer and develop the breeding technology, nursing, and fry
production developed in the subtropical and tropical climate of Pokhara to other sites in Nepal in
order to increase availability of sahar fry for culture.
e. Q uantified A nticipated Benefits:
The results of this study will delineate methods to produce sahar fry for restocking in rivers and
lakes where populations have decreased, and will provide an additional fry for polyculture
systems of Nepal. In Nepal, government labs have initiated fry production for most species, then
trained residents to produce fry for larger scale commercial use. We expect to initiate seed
production at two new sites in Rampur and Kathmandu, and as a result, we should produce
several thousand fry. The promotion of this indigenous fish will increase pond productivity,
harvest, and income. We anticipate at least five farms using sahar in their polyculture systems
when seed become available, as it will add a highly valued fish into the culture system that will
supplement income. Availability of cultured sahar for restocking should lead to decreased fishing
pressure in natural waters. We expect to stock fry in at least five different natural waters as a
result of our seed production. Fry production will also benefit fish culturists in other countries
where sahar is native. For Nepal, we intend to initiate outreach on production of sahar seed by
running at least one workshop on our methods and results. Through this, we intend to train at
least 20 farmers in the technology and aim for at least half of them being women.
f. Research Design:
f.1.
Location of work:
The Fisheries Research Center in Pokhara will serve as a reference site, with the
Aquaculture Department Farm of AFU at Rampur, Chitwan as the new working site.
f.2.
Methods:
f.2.1.
Mature brood stock (1000 g size or larger) will be collected and maintained in Pokhara
and Chitwan.
f.2.2.
Pond facilities: 1 brood pond in Pokhara; and 3 earthen ponds of 200 m2 will be used in
Chitwan.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 8 f.2.3.
f.2.4
f.2.5
f.2.6.
f.2.7.
f.2.8.
f.2.9.
f.2.10.
f.3.
f.3.1
f.3.2
Culture period: One year for mature brood maintenance and breeding.
Nutrient input: Daily feeding with locally made feed containing 35% protein at 23%BW.
Water management: maintain at 1.5 m deep or more. Water quality monitoring will be
done using standard protocol, with monthly water sampling.
We should hold over 600 sahar for broodstock development in Chitwan. We will
monitor oocyte development (using cannulation for a subsample of about 10 fish per
month), gonadosomatic index (GSI of fish that are stripped of eggs at maturation), and egg
somatic index (ESI for these same fish). Temperature and other conditions will be
monitored over the brooding period.
Seasonal maturation patterns will be determined February to March and September to
November.
Egg incubation systems will be developed. We anticipate using hatching jars for
incubation, although we may also try Heath trays.
Fingerlings will be reared in outdoor ponds, first by promoting natural feeds with fertilizer
in the ponds, later using supplemental feed after they have been successfully weaned.
Growth will be estimated over one year, with monthly sampling of fish size and
apparent health for fingerlings being raised in ponds.
Statistical design:
Performance of sahar breeding (OSI, GSI, Maturation patterns) will be compared between
Pokhara and Chitwan hatcheries.
Growth and survival of sahar fry and fingerlings will be compared between the two
locations
Null hypothesis:
There are no differences in breeding and growth performance of sahar between the two
locations (climatic conditions)
Statistical analysis: ANOVA or T-test as appropriate.
g. Schedule Broodstock collection and maintenance will being 1 October 2013, fry rearing is
planned to occur from November 2013 through December 2014. Final report will be completed no
later than 30 September 2015.
University of M ichigan
Nepal Project
Investigation 2
a. T itle: Production of periphyton to enhance yield in polyculture ponds with carps and small
indigenous species (Sustainable feed technology and nutrient input systems/
experiment)
b. Principal Investigators:
Sunila Rai ± Agriculture and Forestry University (AFU), Nepal
Madhav Shrestha ± AFU, Nepal
James Diana ± University of Michigan, USA
Diana; Environmentally sustainable aquaculture systems for Nepal Page 9 c. O bjectives:
c.1. To compare growth and yield of carps between carps and carp-SIS polyculture systems;
c.2. To compare growth and yield of carps and SIS with and without periphyton enhancement;
c.3. To compare gross margin among the different polyculture systems; and
c.4. To assess water quality in different systems.
d. Significance:
The government of Nepal (GoN) has recognized that chronic malnutrition is a major problem in
the country. The most common forms of malnutrition include undernutrition (insufficient
energy), and deficiencies of vitamins and minerals, including vitamin A, iodine, and iron. About
41% of children less than five years of age are stunted (UNICEF, 2012a) and 48% are anemic
(MoHP, 2006). Also, 36% of women, aged 15-49 are anemic (MoHP, 2006). Realizing this,
Baburam Bhattarai, Past Prime Minister of Nepal made a strong commitment to improve the
nutritional status of children and women for future socio-economic growth and development of
the country. To ratify the commitment, the GoN signed the Declaration of Commitment for
Accelerated Improvement in Maternal and Child Nutrition, and launched the Multi-Sectoral
Nutrition Plan (MSNP) on 17 September 2012 (UNICEF, 2012b).
With the current nutrition problem, there is a need to develop environmentally sustainable and
cost-effective food production systems that function year-round to provide adequate nutrients
and improve household income for rural poor farmers. Since 2008, the Institute of Agriculture
and Animal Science (IAAS) has promoted an innovative and environmentally sustainable
KRXVHKROGILVKSURGXFWLRQV\VWHPRI³&DUS-6,6SRO\FXOWXUH´WRLPSURYHQXWULWLRQRISRRUZRPHQ
and children in Terai (Rai, 2012, 2013). The approach includes increased intake of nutrient-rich
Small Indigenous Fish Species (SIS) to improve health and nutrition of women and children.
Vitamin A, calcium, zinc, and iron are found to be much higher in the eyes, head, organs, and
viscera of SIS (Roos et al., 2006). Since SIS are eaten whole, there is no loss of nutrients from
cleaning or as plate waste. Moreover, SIS are self-recruiting in aquaculture ponds after initial
stocking, and can be harvested weekly and biweekly, favoring regular household consumption. A
carp-SIS polyculture system also provides additional income through the sale of surplus fish.
Studies revealed that this farming system raised fish production above that of the national
average, doubled consumption rate of household members, and provided Rs. 3,025 income per
household in 270 days, which helped them to be empowered economically (Rai, 2012).
Adding substrates such as bamboo to carp-SIS ponds can increase carp production and
household income. Since rohu, catla, and common carp are periphyton feeders (Rai and Yi,
2012), their growth and production are enhanced in ponds with added substrate for periphyton
colonization compared to ponds without substrate (Azim et al., 2002; Rai et al., 2008). Azim et
al. (2001) showed a 70% increase in rohu production in ponds with substrates for periphyton,
compared to control ponds. However, gonia ( Labeo gonia ) had no increase in growth, indicating
that the effect of periphyton enhancement is species specific. Azim et al. (2004) showed a 59%
increase in net yield for polyculture carp ponds with feed and periphyton enhancement, and a
28% increase in yield for periphyton enhancement only, compared to ponds with fertilizer only.
However, yields in on-station trials were 77% higher than on-farm trials, indicating that more
work was needed to understand and enhance farm management to achieve desired yields in
Diana; Environmentally sustainable aquaculture systems for Nepal Page 10 periphyton-enhanced production. However, in spite of this difference, they also found that
periphyton enhancement was economically viable for improved production.
The economic value of periphyton enhancement includes the ability to grow fish faster under
similar inputs, as well as the ability to reduce inputs of feed and achieve similar growth rates.
Since the combination of species, type of feed, and other characteristics would influence the
yield and income produced in such a system, it is necessary to test the full combination of feed
inputs, periphyton enhancement, and production to truly understand the best system to use for
commercial production (Diana, 2012). Therefore, we will compare production among systems
with no SIS, no periphyton, and normal feeding (control) to systems with SIS and normal
feeding; periphyton enhancement, SIS, and reduced feeding; and SIS, periphyton enhancement,
and no feeding. This should give a reasonable indication of the full potential for changes in costbenefit ration considering inputs of fish, fertilizer, and feed.
The study is intended to compare fish production between normal carp-SIS polyculture and
periphyton-enhanced carp-SIS polyculture in order to develop a cost-effective means to increase
fish production. Since on-farm trials have shown significant reductions in production compared
to on-station trials, we will also evaluate performance differences between farm and station
systems.
e. Q uantified A nticipated Benefits:
The results of this study will develop a more sustainable carp-SIS polyculture system suitable to
small-scale farmers in Nepal. We anticipate that the polyculture system with periphyton will
increase on-farm fish yield by at least 20%, which should improve family income and also
increase household fish consumption, particularly that of SIS. This will benefit farmers through
improved family nutrition, health, and livelihood. In addition, famers will have the opportunity
to harvest and consume SIS from their ponds for year-round consumption because in carp-SIS
production, system ponds are not completely drained during final harvest. Since SIS grow well in
shallow water, they can be produced in the dry period when carp polyculture is not feasible. Onfarm trials are planned for 20 farms in each location, so that at least 40 farmers will be exposed
to these new combinations to try in their household ponds.
f. Research Design:
f.1.
Location:
First an on-station trial will be done at AFU, then on-farm verification of the best
V\VWHP ZLOO EH WHVWHG LQ IDUPHUV¶ SRQGV LQ WZR GLVWULFWV DW 0DMKXL &KLWZDQ DQG
Kawasoti, Nawalparasi.
f.2.
Methods:
f.2.1.
AFU Pond Research
f.2.1.1.
Pond facility: 12 earthen ponds of 150 m2 will be used for on-station trials
f.2.1.2.
Culture period: 6 months each for on-station and on-farm trials.
f.2.1.3.
Test species: carps (common, silver, bighead/catla, grass, rohu, mrigal) and SIS
(dedhuwa and pothi)
f.2.1.4.
Stocking and treatments
Stocking size: carps (5-10 g), SIS (2-5 g)
Diana; Environmentally sustainable aquaculture systems for Nepal Page 11 O n-station T rial:
The on station trial will include 4 treatments with 3 replicate ponds each:
(1) Carp polyculture (15000/ha) with normal feeding (control)
(2) Control + SIS with normal feeding (50000/ha)
(3) Control + SIS (50000/ha) + bamboo substrate (covering 1% of pond surface area)
with 50% reduced feeding
(4) Control + SIS (50000/ha) + bamboo substrate (covering 1% of pond surface area)
with no feeding
f.2.1.5.
f.2.1.6.
f.2.1.7.
f.2.1.8.
O n-farm Verification:
The best two carp-SIS polyculture systems obtained from the on-station trial will be
verified in two districts, Chitwan and Nawalparasi, covering twenty household ponds
in each district.
Nutrient input: Fertilization and daily feeding 6 days per week with dough of rice
bran and mustard oil cake at 2%BW for most carps and grass to grass carp at 5% BW.
Water management: maintain at 1 m deep.
Sampling schedule: Water quality: Biweekly water sampling, Standard CRSP
protocol. Fish growth: monthly sampling of carps only. Partial harvesting: Monthly
after first breeding of brood SIS.
Statistical design, null hypothesis, statistical analysis:
Statistical design: Completely randomized design (CRD)
Statistical analysis: Multiple ANOVA
Null hypothesis: There are no differences in growth, production, gross profit margin,
and partial harvest among different polyculture systems (control, with SIS, with SIS
and periphyton, with SIS, periphyton and no feeding).
g. Schedule:
AFU experiment will begin 1 March 2014 and run 180 days; the report will be submitted no later
than March 2015. On-farm trials will begin 1 March 2015 with final report no later than 30
September 2015.
University of M ichigan
Nepal Project
Investigation 3
a. T itle: Household fish ponds in Nepal: their impact on fish consumption and health of women
and children; their constraints determined by value chain analysis; and their extension
WKURXJKVFKRROSRQGVDQGZRPHQ¶VILVKIDUPLQJJURXSV (Human nutrition and human
health impacts of aquaculture/study)
b. Principal Investigators:
James Diana ± University of Michigan, USA
Narayan P. Pandit ± Institute of Agriculture and Animal Science (IAAS), Nepal
Ram B. Mandal ± IAAS, Nepal
Dilip K. Jha ± AFU, Nepal
Madhav Shrestha ± AFU, Nepal
Diana; Environmentally sustainable aquaculture systems for Nepal Page 12 c. O bjectives:
c.1. To conduct an analysis of household pond production, harvesting, processing, distribution,
markets, and sales in Southern Nepal;
c.2. To organize data into a value chain diagram and to use the diagram to draw insight
into possible improvements in the aquaculture sector.
c.3. To determine the frequency and amounts of fish eaten by children ages 1-5, as well as
women, from households with or without household fish ponds;
c.4. To evaluate the health characteristics of children from households with or without
household fish ponds;
c.5. To establish school ponds in villages for education of school-age children on the value of
household ponds; and
c.6. 7RGHYHORSZRPHQ¶VILVKIDUPLQJJURXSVDWHDFKVFKRROYLOODJHWRWHDFKWKHPDERXWILVK
farming and household health.
d. Significance:
The government of Nepal has recognized that chronic malnutrition is a major problem in the
country. The most common forms of malnutrition include undernutrition (insufficient energy)
and deficiencies of vitamins and minerals, including vitamin A, iodine, and iron. About 41% of
children less than five years of age are stunted (UNICEF, 2012a) and 48% are anemic (MoHP,
2006). Also, 36% of women, aged 15-49 are anemic (MoHP, 2006). Realizing this, the GoN
signed the Declaration of Commitment for Accelerated Improvement in Maternal and Child
Nutrition and launched the Multi-Sectoral Nutrition Plan (MSNP) on 17 September 2012
(UNICEF, 2012b). Much of our research and outreach in Nepal has focused on providing fish
culture alternatives to improve the nutrition and health of poor farmers, but we have not yet done
much to assess the success of increased fish production on human health.
Fish has been recognized as a nutritionally beneficial food source around the world. It has long
been known that fish provide high quality protein and important micronutrients, such as vitamin
A, vitamin D, and iodine, and they can also be a source of phosphorus, fluoride, and calcium if
bones are consumed (Speedy, 2003). Additionally, the benefits of consuming fish for Omega 3
fatty acids has recently been widely documented (Oken and Belfort, 2010; Mahaffey et al.,
2011). While certain fishes can provide all of these health benefits, there currently exists a
difference in the perceived nutritional gains in developed versus developing countries: in the
former, individuals, the media, and researchers are primarily concerned with Omega 3 fatty acids
(Domingo et al., 2007; Oken and Belfort, 2010), while in the latter, the primary concerns are
protein and micronutrients (Parajuli et al., 2012; Aiga et al., 2009). In Nepal, the benefits of fish
consumption have been linked with such outcomes as improving protein intake (Bhujel et al.,
2008) and increasing vitamin A and zinc ingestion (Parajuli et al., 2012). Little data exist in
relation to fish production in Nepal, but approximately half of all fish produced in 1994/1995
was done so through aquaculture (FAO, 2012). It is believed that the majority of fish currently
consumed in Nepal is produced through aquaculture practices due to the fact that nearly all fish
sold in markets in Kathmandu and surrounding areas are raised in ponds.
During summer 2012, we conducted our first study on the influence of household ponds on the
health and nutrition of children in the household (Stepan, 2013). This study focused on small
household ponds in Kathar and Kawasowoti, with a control population in Bhandara. The concept
Diana; Environmentally sustainable aquaculture systems for Nepal Page 13 of small household ponds was originally extended to local residents to improve the nutrition of
poor families in Nepal. These ponds have been so successful by local residents that the number
of ponds has increased from around 100 in the early stages to over 1,000, with the additional
ponds built by local owner groups. All of the adopting communities are in the Terai region and
are comprised mainly of Tharu people. The earlier study showed that residents with household
ponds consumed about five times more fish than children in households without ponds. While it
was clear that fish consumption did increase dramatically in households with ponds, it was less
clear that this consumption resulted in increases in the height at age or weight at age for children
from those households, or in the health of mothers or pregnant women. This was due in part to
problems with the timing and intensity of our sampling; in addition, it was affected by the similar
socioeconomic status of all participants. However, the survey did help detect some consistent
patterns and also helped us to design better surveys for the future. One purpose of this study is to
conduct such an expanded survey.
One issue related to the expected health improvements from people eating fish would be what
other sources of protein are available to them. Most Nepalese families eat a largely vegetarian
diet, focused on rice and some vegetables, with fish or meat added when available (Stepan 2013).
The Terai region of Nepal is its main agricultural area, with much production of rice as well as
some livestock. So health improvements might not be measureable if households without ponds
eat meat instead of fish. Our earlier survey had some flaws, mostly in the timing of sampling (in
summer, eight months since the last fish harvest) as well as in finding sufficient families with
young children (under the age of five) whose growth trajectories would be reflected by recent
consumption history. In this study we intend to improve on these limitations by also measuring
the amount of meat consumption, and by sampling more families so we can have adequate
numbers of children under five.
Although aquaculture has been practiced in Asia for thousands of years (FAO, 2012), it is fairly
new in Nepal. It was not until the 1940s that the country began raising fish, and an additional 40
years passed before any significant progress was made in the field (FAO, 2012). Considering
1HSDO¶V ODWH VWDUW LQ DTXDFXOWXUH SUDFWLFHV LW LV QR VXUSULVH WKDW WKH FRXQW\ LV \HW WR FRQWULEXWH
substantially to the huge volume of Asian aquaculture production (Asia produced 92.5% of the
worOG¶V WRWDO DTXDFXOWXUH LQ ) or benefit largely from the economic improvements that
aquaculture has been shown to create (NACA, 2010). Recently, however, Nepal has shown
marked increase in carp polyculture (FAO, 2012). We have completed a value chain analysis
(VCA) of this system (Stepan et al., 2013), so for our next evaluation we will evaluate household
ponds as the target. While these ponds function mainly for family consumption rather than sales,
the end use of the fish produced, as well as the methods for fingerling acquisition and other
transfers of fish, have not been studied. Because these ponds are so popular, the second purpose
of this proposal is to conduct a VCA on this system of aquaculture.
The general concept of a value chain is to link all steps of production, processing, and
distribution of a product together, and then to analyze each step as it relates to those that precede
and follow it. In doing so, the value chain describes all of the activities responsible for bringing a
product from creation to ultimate disposal (Hempel, 2010). Given the early stages of aquaculture
development in Nepal, a value chain would be best applied to evaluating limitations in the
Diana; Environmentally sustainable aquaculture systems for Nepal Page 14 potential growth of the sector. Taking this into consideration, the following topics have been
highlighted as research areas:
x Fingerling production ± Is there adequate fingerling production to accommodate the
demand for cultured fishes in Nepal? Are fingerlings wild caught?
x Seasonality ± Rural lowland communities in Nepal are able to raise fish only in the
monsoon season when water is plentiful. How does this restraint affect fish production?
x Transportation ± How far away can fish be safely transported?
x Market concerns ± What is the nature of the demand for cultured fish in Nepal?
Women play an integral role in the aquaculture and fisheries sectors all over the world. Even
WKRXJKZRPHQ¶VUROHVDQGUHVSRQVLELOLWLHVDUHFKDQJLQJLQVRPHFRXQWries, there are constraints
that limit female participation in aquaculture (Egna et. al., 2012). A few such constraints that
women face in aquaculture and fisheries are: time availability and allocation, land ownership,
and access to water, credit, training, and labor. Lack of training opportunities can trap women in
vulnerable and poorly paid positions with no prospects of getting ahead (FAO, 1998). However,
the situation in Nepal with household ponds differs considerably from this norm. In most of the
poorer Nepalese households, women tend and manage gardens and ponds, while men seek work
at outside locations (Bhujel et al., 2008). Therefore, household ponds enhance the income,
nutrition, and status of women, and provide them with alternatives for their families.
We propose to use school farms and education on the nutritional value and methods of
aquaculture to help young people understand the value of fish production and consumption for
their families. While many Nepalese attend school, most have only a primary school education
and about 68% of women are illiterate. Therefore, training must consider these limitations while
still providing for information exchange (Kloblen 2011). Schools remain the center for learning
in the community. Having ponds in the schools will help disseminate a practical message to the
local population that fish are an important constituent to boost nutrition, and hence, residents will
be encouraged to build a fish pond of their own. This will also help in capacity building of
teachers who could spread knowledge of the importance of fish in nutrition to parents during
teacher-parent interactions. Finally, income from the sale of fish will aid poor schools in
developing infrastructure and covering daily expenses.
In addition to the education of children, the school ponds can be used to train a group of women
in each community on the methods of aquaculture and the value of fish consumption to their
families. :HSODQWRGHYHORSDZRPHQ¶VILVKIDUPLQJJURXSLQHDFKVFKRROFRPPXQLW\WRIurther
extend fish farming information to local women. Since many of these women are illiterate, it will
be necessary to develop special messages with clear visual and practical solutions to information
exchange.
This study is intended to focus on the value of household ponds in three ways: 1) to compare fish
consumption and indicators of health for children and women in households with fishponds to
those without access to ponds; 2) to complete a value chain analysis of household ponds; and 3)
to use schooO SRQGVDQGZRPHQ¶VILVKIDUPLQJ JURXSV DVPHDQVWR IXUWKHUH[WHQGWKHYDOXHRI
household ponds into rural communities. To expand the reach of this study, we have initiated
collaboration with Winrock International and the Nutrition CRSP, two other NGOs concerned
with human nutrition in Nepal. We anticipate that they will at least aid us in designing survey
Diana; Environmentally sustainable aquaculture systems for Nepal Page 15 tools to complete this work, and we hope they will conduct similar surveys and outreach in other
regions or for other types of consumers to gain an even larger database on consumption and
nutrition in Nepal.
e. Q uantified A nticipated Benefits:
This study will provide a robust database on the nutrition of children in rural Nepal families and
the role of fish consumption in their health. We intend to survey at least 200 families and, as a
result, we anticipate that all of these families will gain a better understanding of nutrition and the
role of protein in the health of their children. The results of this survey will also inform
aquaculture extension in the country, as it will indicate whether ownership of small household
ponds is truly aiding in the nutrition of these families.
The completion of a VCA for household ponds will provide better understanding of their
strengths and weaknesses. Through this understanding, all of those involved in the sector, from
fingerling producers to fish farmers to those possibly involved with fish sales, will be informed
of management strategies to improve their service. This will lead to increased supply of highquality fish protein to communities with limited food resources, and the overall growth of the
aquaculture sector.
7KHGHYHORSPHQWRIVFKRROSRQGVDQGZRPHQ¶VILVKIDUPLQJJURXSV will increase awareness of
the value of nutrition and fish consumption in rural households by teaching school-age children
and adult women about aquaculture. It will help generate income for the families and schools
having ponds. It will also help in capacity building of teachers who could spread the knowledge
on the importance of fish in nutrition to parents during teacher-parent interactions. We anticipate
that at least 4 school ponds will be built, 40 or more students will be educated on the methods of
fish farming, and 20 women will receive training in fish farming and its role in household health.
f. Research Design:
Household pond surveys and V C A
f.1
Location:
Since most aquaculture is done in the Terai region, we will focus our surveys there.
We will use the advice of Winrock and Nutrition CRSP colleagues, as well as faculty
at AFU, to determine the best locations to focus these surveys. We do intend to
survey at four different parts of the Terai.
f.2
Methods:
f.2.1
Social science survey of nutrition and value chain.
f.2.1.1
We intend to survey 50 households in each of the four locations selected.
f.2.1.2
Our household surveys will evaluate the production details (source of fry, end use of
fish) for household pond owners as part of the VCA.
f.2.1.3
Once we have identified likely sources or markets for fish produced in household
ponds, we will survey those participants as well to complete the value chain details.
f.2.1.4
Social science survey instruments will be designed to determine the frequency and
amounts of fish eaten by children ages 2-5 as well as by women in the household.
Special attention will be paid to the consumption of SIS, in addition to total fish
consumption.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 16 f.2.1.5
f.2.1.6
f.2.1.7
f.2.1.8
f.2.2
f.2.2.1
f.2.2.2
f.2.2.3
f.2.2.4
f.2.2.5
f.2.3
f.2.3.1
f.2.3.2
f.2.3.3
We will monitor the height and weight of children from households surveyed, when
possible, and compare these statistics among households with or without ponds as
well as with national data.
Value Chain Mapping will be done with data collected on uses and sources.
Analysis of opportunities and constraints: Following the previous steps, opportunities
for improvement and specific constraints of the household pond value chain will be
described in a final report.
Statistical design, null hypothesis, statistical analysis:
Statistical design: Completely randomized design (CRD)
Statistical analysis: One way ANOVA between fish pond owners and non-owners of
each region. In addition, multivariate analysis will be done to connect socioeconomic
indicators and family health. Test variables will include daily fish consumption, SIS
consumption, height at age, weight at age, and weight at height for each participant.
Null hypothesis: There are no differences in health indicators or fish consumption
between participants who own fish ponds and those who do not own ponds.
School ponds and women groups
Establishment of demonstration school ponds for fish farming and nutrition education
Public schools will be selected where household fishponds are used in Kathar and
Kawasoti.
A 200 m2 pond will be constructed for two schools in each district.
Carps and tilapia will be stocked in each pond and the materials necessary to grow
them will be provided to each school system.
School students and teachers will receive regular training about pond construction
and farming activities.
Focal educational activities include: fish pond construction; fish farming, including
feeding, fertilizing, growing and handling fish; and nutrition education, including fish
cooking and eating.
FRUPLQJDZRPHQ¶VILVKIDUPLQJJURXS.
Biweekly meetings and discussions on fish farming will be held with groups of
women established at each target school
Topics on fish farming will be extended, including managing pond depth, pond
preparation, species choice, water color, fertilizing, feeding, growing, and harvesting
of fish.
Topics on health and nutrition will also be extended, including fish preparation and
the value of regular consumption.
g. Schedule:
Since ponds are harvested mainly in the fall, our field surveys will be done from 1 September 2014
through 1 December 2014, with final reports on nutrition and VCA due 1 September 2015. School
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expected by October 2014 and final reporting due 30 September 2015.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 17 University of M ichigan
Nepal Project
Investigation 4
a. T itle:
Introduction of two small indigenous species to improve sustainability in typical
polyculture systems in Nepal (Production System Design and Best Management
Alternatives/experiment)
b. Principal Investigators:
Bailey Keeler ± University of Michigan, USA
James S. Diana ± University of Michigan, USA
Narayan P. Pandit ± Institute of Agriculture and Animal Science (IAAS), Nepal
Ram B. Mandal ± IAAS, Nepal
Madhav K. Shrestha ± Agriculture and Forestry University (AFU), Nepal
c. O bjectives:
c1.
To evaluate the impact of adding different densities of two small indigenous fish species
(Punti Puntius sophore and Deduwa Esomus danricus) to the yield and economic
performance of the 6-species carp polyculture system in Nepal; and
c2.
To determine the impacts of adding new species on water quality and primary production
in polyculture ponds.
d. Significance
Carp polyculture is the most common aquaculture system in Nepal and dominates total
production. Most fish harvested by pond owners are used for market sales; thus, they do not enter
largely into household consumption. However, inclusion of Small Indigenous Species into
polyculture has been suggested to help these systems contribute to household consumption and
nutrition. The intake of nutrient-rich SIS should improve the health and nutrition of women and
children. Vitamin A, calcium, zinc, and iron are found to be much higher in the eyes, head,
organs, and viscera of SIS (Roos et al., 2007). SIS have a faster reproductive rate than carp
species and have been known to breed in culture ponds (Kadir et al., 2006). They can therefore
be harvested throughout the grow-out season, adding consistently to household consumption,
even during times of low water levels. A carp-SIS polyculture system also provides income
through the sale of surplus carp. This farming system raised fish production above that of the
national average, doubled consumption rate of household members, and provided Rs. 3,025
income per household in 270 days, which helped them to be empowered economically (Rai,
2012). Adding substrates such as bamboo to carp-SIS ponds can increase carp production and
household income. Since rohu, catla, and common carp are periphyton feeders (Rai and Yi,
2012), their growth and production are enhanced in ponds with added substrate for periphyton
colonization compared to ponds without substrate (Azim et al., 2002; Rai et al., 2008). Similar
results have been found in Bangladesh, as well (Wahab et al., 2003)
While SIS have been added to polyculture ponds and the results to date seem favorable, there has
been little experimentation on the density of SIS to use and the resulting production of SIS from
these ponds for household consumption. Once introduced to the pond, most species of SIS will
naturally breed there and self-recruit to the population. Therefore, the density of SIS needed to
Diana; Environmentally sustainable aquaculture systems for Nepal Page 18 induce full breeding and recruitment is an important criterion to the enhancement of production
that they can induce. This project will expand on our research incorporating SIS by exploring the
feasibility of adding SIS at different densities to a typical culture system in Nepal.
Periphyton has been shown to increase production of various carp species (Azim, et al. 2001;
2002). Earlier experiments (Investigation 2) were designed to test the importance of substrates to
production of periphyton and carp in polyculture ponds. In support of that study, we will use
bamboo substrates in this experiment to allow this enhanced periphyton production, and test the
stocking ratios of SIS.
e. Q uantified A nticipated Benefits.
The target end users of this system are small-scale rural farmers and their families in the Terai
region of Nepal. We anticipate that the addition of SIS to this culture system will increase yield
by at least 20%, without reducing carp production. The large carp species are commonly
FRQVLGHUHG ³FDVK FURSV´ DQG DUH VROG LQ ORFDO PDUNHWV DV ZHOO DV FRQVXPHG 6,6 ZRXOG VHUYH
principally as a regular food source for farmers. SIS addition to large carp culture will directly
improve the nutritional options for farmers and their families. We believe that SIS produced in
the ponds will increase household fish consumption by women and children by at least 2 fold.
f. Research Design:
f.1.
Location:
The ponds will be located in the Chitwan region of Nepal and will be earthen
experimental ponds in Kathar. The farms will be owned by local residents, but
managed by the research group. Farm families will derive the benefit of SIS
consumption and carp sales.
f.2.
Methods:
f.2.1.
Pond Research
f.2.1.1.
Pond facility: 12 earthen ponds of 100 m2 will be used
f.2.1.2.
Culture period: 6 months.
f.2.1.3.
Test species: Carps (common, silver, bighead/catla, grass, rohu, mrigal) and SIS
(dedhuwa and pothi)
f.2.1.4.
Stocking and treatments
Stocking size: Carps (5-10 g; stocked at 15,000/ha), SIS (2-5 g)
(1) Carp polyculture alone (control)
(2) Control + SIS (25,000/ha)
(3) Control + SIS (50,000/ha)
(4) Control + SIS (100,000/ha)
f.2.1.5.
Nutrient input: Fertilization and daily feeding 6 days per week with dough of rice
bran and mustard oil cake at 2%BW for most carp and grass to grass carp at 5% BW.
Bamboo stakes will be used as additional substrate in all treatments in order to
promote periphyton colonization.
f.2.1.6.
Water management: maintain at 1 m deep.
f.2.1.7.
Sampling schedule: Water quality: monthly water sampling, Standard CRSP protocol.
Fish growth: monthly sampling of carps only. Partial harvesting: Monthly after first
breeding of brood SIS, remove one seine haul of SIS per week. Weight of SIS
harvested will be recorded.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 19 f.2.1.8.
Statistical design, null hypothesis, statistical analysis:
Statistical design: Completely randomized design (CRD)
Statistical analysis: One-way ANOVA
Null hypothesis:
1. There are no differences in growth, production of carp, and gross profit margin
among different polyculture systems.
2. There are no differences in biomass produced or biomass serially removed of SIS
from ponds stocked at different densities.
g. Schedule
Experiment will begin 1 June 2013 and run 180 days; the report will be submitted no later than June
2014.
University of M ichigan
Nepal Project
Investigation 5
a. T itle: Using standard methods to evaluate experimental seed production and culture
techniques for Singhi ( Heteropneustes fossilis, Bloch) in Nepal (Quality Seedstock
Development/activity)
b. Principal Investigators:
C.N.R. Yadav ± Agriculture and Forestry University (AFU), Nepal
Madhav K. Shrestha ± AFU, Nepal
James S. Diana ± University of Michigan, USA
c. O bjectives:
c.1. To produce seed of Singhi using artificial maturation techniques; and
c.2. To attempt a grow-out of Singhi in a pond environment using artificial and natural feeds.
d. Significance:
Nepal has vast freshwater resources covering nearly 826,600 ha (FDD, 2009/2010). Aquaculture
is one of the most efficient ways to use these waters. Besides the main cultured indigenous and
exotic fish species, several high valued local fishes are also preferred for their excellent taste
(Shrestha, 1994). Among local fishes, Singhi ( Heteropneustes fossilis) is an economically valued
catfish in Nepal. It is an obligate air-breathing fish, reported to be highly nutritive and
recuperative, and it has medicinal properties. This fish is esteemed for its invigorative qualities
(Alikunhi, 1957). It is rich in protein and iron, having low fat content (Yadav and Bart, 2003).
Maximum size of Singhi has been reported at 38 cm. Males are smaller than females. In one
year, females grow to 12 cm, and males to only 8 cm (Sinha, 1993). It inhabits ponds, ditches,
swamps and marshes, derelict water areas, and sometimes even muddy rivers. It survives these
conditions because of its accessory breathing organs in the form of air tubes. Unlike waterbreathing fish, it can be easily moved and transported live to consumers (Prasad et al., 1993).
This feature attracts the consumer and fetches a good return to the grower (Yadav, 1999).
Tharakan and Joy (1996) reported that this fish is ideal for waste-water aquaculture. Being
bottom dwelling and omnivorous, Singhi is fond of insects, ostracods, worms, zooplankton,
Diana; Environmentally sustainable aquaculture systems for Nepal Page 20 macrophytes, algae, organic debris, and fish (Alikunhi, 1957). It accepts supplementary feed as
well. Optimum temperature for successful breeding of Singhi is 22°C, and it does not breed
below 10°C or above 30°C (Saxena and Sandhu, 1994). This fish is a seasonal breeder, usually
spawning during July and August (Chatterjee et al., 1991). Sexual maturity is attained at the end
of the first year. Fecundity varies from 3,000 to 45,000 eggs (Azadi and Siddique, 1986;
Burgess, 1989; Sinha, 1993), and a 100g female usually produces 8,000 eggs.
Commercial cultivation of Singhi is not common in Nepal, although people have been
consuming it from wild fisheries. The catch of Singhi is either from naturally occurring culture
ponds or natural waters. Presently, the source of fry for Singhi is collection of wild seed, which
does not ensure its quality or quantity. While seed can also be produced by breeding the fish
under controlled conditions, this is not done commercially. Adapting appropriate breeding and
culture techniques for large-scale production is an important need for Singhi cultivation. The
purpose of this project is to initiate research on methods for quality seed production at a largescale and successful culture of Singhi up to marketable size.
e. Q uantified A nticipated Benefits:
This study will explore the culture and breeding methods for Singhi in ponds, which will initiate
a new avenue for aquaculture in Nepal. We anticipate developing methods to produce at least
1,000 fry in year 1, and growing them to market size by the end of year 2. This will provide one
new culture system in Nepal, as well as methods to produce fry for the species.
f. Research Design:
A quaculture Study
f.1
Location: AFU.
f.2
Methods:
f.2.1
AFU Pond Research
f.2.1.1
Pond facility: 1 earthen pond of 150 m2
f.2.1.2
Culture period: 10 months from July to May.
f.2.1.3
Test species: monoculture of Singhi
f.2.1.4
Stocking size: 5-10 g
f.2.1.5
Stocking density 20,000 fish/ha
f.2.1.6
Feeding rate: Rice bran, trash fish, and oilcake ad-libitum at night using a feeding
basket
f.2.1.6
Sampling schedule: Monthly water quality sampling, standard CRSP protocol. Fish
growth: monthly sampling.
f.2.1.7
Statistical design, null hypothesis, statistical analysis:
Statistical design: none; demonstration project
(ii) Seed Production:
Brood fish will be collected and kept in the earthen ponds at AFU. They will be fed with proteinrich supplementary feed ad-libitum at least 3-4 months prior to spawning.
Inducing hormones and breeding strategy:
Two inducing agents will be tried separately to ascertain their efficacy: Ovaprim and Carp
pituitary extract (CPE). Only female fish will be injected with Ovaprim at 0.5ml/kg and CPE at
Diana; Environmentally sustainable aquaculture systems for Nepal Page 21 200mg/kg body weight using a single dose. The fish will then be released into a hapa within a
pond for semi-artificial breeding. A small inner hapa (made of coarse net cloth) and larger outer
hapa (made of fine cloth) will be used. Females and males (1:1 ratio) will be released into inner
hapa, and eggs will be collected from the outer hapa. The released fish will be left undisturbed
and spawning will be monitored from 12 hours onwards after hormone administration. After
spawning, brood fish will be removed along with the inner hapa. The eggs in the outer hapa will
be left for natural hatching. Collected eggs, hatchlings, and fry will be counted and their survival
rates will be assessed. Feeding will commence four days after hatching. Yolk of boiled poultry
eggs will be used in the first feeding. After the end of the first week, the fry will be transferred
from the hapa to a nursery tank. Poultry eggs will be replaced by minced gastropods in feeding,
and 5-10g fingerlings will be stocked in grow-out ponds for producing marketable sized fish.
g. Schedule:
Brood rearing will begin 1 September 2013 continue throughout the study. Fry production will
hopefully begin in July 2014, and fry rearing begin immediately afterwards. A final report will be
submitted no later than June 2015.
University of M ichigan
Nepal Project
Investigation 6
a. T itle: Demonstrating the value of tilapia and sahar production in polyculture ponds using
government farm and on-farm trials (Production System Design and Best
Management Alternatives/ study)
b. Principal Investigators:
Ramananda Mishra ± Directorate of Fisheries Development, Nepal
Madhav K. Shrestha ± Agriculture and Forestry University, Nepal
James S. Diana ± University of Michigan, USA
c. O bjectives:
c.1 To increase pond productivity through species diversification;
c.2 To demonstrate a carp-tilapia-sahar polyculture system for outreach potential by
government fisheries development program;
c.3 To demonstrate the culture potential of sahar and tilapia to the farmers; and
c.4 To develop partial enterprise budgets of variable costs and value of fish crops among
treatments
d. Significance:
Total fish production in Nepal is 54,357 mt, with about 60% originating from aquaculture. Pond
culture is the most popular method of aquaculture, but annual pond yield averages only 3.83 t/yr
(DoFD, 2012). Carps are popular warmwater fish for culture in Nepal, contributing more than
99% of aquaculture production in the country. Tilapia, which is also referred to as the aquatic
chicken, is a globally prominent species for all types of management intensities. Nile tilapia
( Oreochromis niloticus) was introduced into Nepal in 1985 (Pantha, 1993), but it remained under
government control for more than 10 years (Shrestha and Bhujel, 1999). Since 1996, experiments
Diana; Environmentally sustainable aquaculture systems for Nepal Page 22 conducted at IAAS included polyculture of tilapia and common carp (Shrestha and Bhujel,
1999), mixed-size culture of tilapia (Mandal and Shrestha, 2001), and polyculture of grass carp
and tilapia (Pandit et al., 2004). As mixed-sex tilapia is most commonly used for culture,
recruitment control remains a problem. Snakehead (Yi et al., 2004) and sahar (Shrestha, 1997)
have been evaluated for their ability to control tilapia reproduction by predation on tilapia fry.
Tilapia and sahar co-culture was attempted to control excessive recruitment of tilapia and also to
provide an additional species to increase productivity of high valued fish that are indigenous
(Shrestha et al., 2011). Experiments indicated that sahar can control tilapia fry production
(Poudel et al., 2007; Rai et al. 2007, Yadav et al., 2007; Shrestha et al., 2011). Growth of sahar
was higher in tropical and subtropical ponds than in cages reared in Pokhara lakes and also
suspended cages in ponds (Bista et al., 2001, 2007; Shrestha et al., 2005, 2007). Sahar has been
overfished in rivers and lakes with declining populations (Rai et al., 2007; Rajbanshi, 2001; Joshi
et al., 2002).
Semi-intensive carp polyculture is an established system in the tropical and subtropical regions
of Nepal, using fertilized ponds with supplemental feed. The carp species include common,
silver, bighead, grass, naini/mrigal, and bhakur/catla. All seven species are recommended in
certain ratios with a combined density of 10,000 fish/ha, but fingerlings of all species are rarely
available when needed for stocking. The typical number of species cultured ranges from four to
six. The addition of other proven species (such as tilapia and sahar) with increased stocking
density into the existing carp production system could increase productivity up to 57% and net
returns by 61% (Shrestha et al., 2012).
The addition of new species to the carp polyculture system fits both the national aquaculture
plans elaborated by as well as the Feed the Future plans for aquaculture improvement. The first
FtF research goal is to advance the productivity frontier by both increasing productivity beyond
current levels through technology development, and extending technology so that local
production can reach the level of research farms. This proposal will focus on both of those goals.
Secondly, the national plans for aquaculture and fisheries have goals to improve culture of
indigenous fishes, as well as to raise yield of ponds from farms to the level of research stations
(GoN 2000, NARC 2010). Again, this project is in complete alignment with those goals.
This study is intended to expand the technology developed through AquaFish CRSP research on
carps, tilapia, and sahar production to farmers in order to demonstrate alternative fish production
models. In particular, we will produce fish in a polyculture system on a government farm using
all 7 species, and determine the partial enterprise budget for the production system. After this
demonstration, we will conduct an on-farm experiment on polyculture systems, using carps, with
addition of tilapia or tilapia and sahar to determine the most practical system for farm adoption.
e. Q uantified A nticipated Benefits:
The results of this study will provide two additional species in the polyculture system of Nepal,
which should increase production and income. It will add high valued fish to the culture system
and supplement income. As carp polyculture is established, the increasing species will be easier
to adopt by fish farmers. It will also help in production of sahar and decrease fishing pressure in
nature. It will benefit fish culture in south Asia and other countries where carp culture is popular.
The immediate impact will be measured by the increased production and economic returns in onDiana; Environmentally sustainable aquaculture systems for Nepal Page 23 farm trials for the different polyculture systems. We expect to improve yield and economic
returns for polyculture systems by at least 30% with these additions, and train at least 5 farmers
in the new production system.
f. Research Design:
f.1
Location: Fisheries Development Center, Bhairahwa and Dayanagar, as well as farm
ponds in fish production pockets of the Rupandehi district.
f.2
Methods:
f.2.1
Pond Demonstration:
f.2.1.1
Pond facility: 15 earthen ponds of 200-500 m2 will be used for both government (onstation) and on-farm ponds.
f.2.1.2
Culture period: 10 months each.
f.2.1.3
Test species: Carps (Common, Silver, Bighead, Grass, Rohu, Mrigal and Catla), Nile
tilapia (mixed sex) and Sahar
f.2.1.4
Stocking:
Carps (5-10 g) at 10,000/ha
Mixed-sex Nile tilapia (5-10 g) at 3,000/ha
Sahar (5-10 g) at 1,000/ha
f.2.1.5
Nutrient input: Fertilization and feeding with locally made feed at 2%BW
f.2.1.6
Water management: maintain at 1 m deep.
f.2.1.7
Sampling schedule
Water quality: Biweekly measurement of temperature, DO, pH, transparency
Fish growth: monthly sampling
f.2.1.8.
Statistical design, null hypothesis, statistical analysis:
This is a demonstration and has no treatments and no statistical analyses.
f2.2
On-farm trial:
f2.2.1
The on-farm trial will include three treatments with five replicate ponds each. Most
methods will be similar to those listed above. The treatments will be:
f2.2.1.1 Existing carp polyculture (10,000/ha) (control)
f2.2.1.2 Control + tilapia (3000/ha)
f2.2.1.3 Control + tilapia (3000/ha) + sahar (1000/ha)
f.2.1.8.
Statistical design, null hypothesis, statistical analysis:
The trial will be conducted in a completely randomized design, and data will be
analyzed using one-way ANOVA.
Null hypothesis: Introduction of tilapia and sahar in carp polyculture has no effect on
growth, production, or economic return in different polyculture systems.
g. Schedule: On-station April to November 2014 on-farm April to November 2015. Final report no
later than August 2015.
O U T R E A C H A N D D ISSE M I N A T I O N P L A N
The AquaFish Innovation Lab intends to develop outreach to affect four different communities:
end users; host country decision makers, including government, researchers, and extensionists;
USAID personnel; and other stakeholders, mainly including the broader educational and user
community. We intend to focus our efforts on these four groups in an appropriate and targeted
way for each user, as defined below.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 24 We have considerable experience throughout Southeast Asia in moving our aquaculture research
to farmers and other end users by development of outreach materials, by training through
workshops and short courses, and by involvement of the NGO community. In Nepal, this is
limited by one serious problem ² that illiteracy is so widespread, causing more need for inperson training and less for developing written outreach materials. Our investigations described
earlier all had some form of outreach planned. These included at least 60 farms or households
doing on-farm trials; RYHUSHRSOHUHFHLYLQJ GLUHFW WUDLQLQJWKURXJKVFKRRO SRQGVZRPHQ¶V
groups, or direct intervention at the farm; formation of four school ponds and four ZRPHQ¶VILVK
farming groups to disseminate information; and other ancillary activities. AFU has a mission to
provide training to farmers, as well as to extension and government agents on the research done
by their faculty or, in this case, under the AquaFish Innovation Lab. The main avenue for such
training has been short courses at the host institutions. We intend to continue these short courses
with the current research. Additional deliverables in this area include one workshop or other
educational activity to be conducted on each investigation during the first two years of this
collaboration. Through these workshops we will extend our information mainly to end users in
private business.
One important means to multiply our technology transfer is to include those already providing
such service in Nepal. To this end, we are teaming with NARC, Winrock International, and the
Nutrition CRSP to further extend our results to the larger community. We intend to work closely
with additional NGOs in Nepal to ensure the widest distribution of our research results. One
method of outreach to these professional groups is involvement in country and regional meetings
of scientists and extensionists, and we intend to send faculty from AFU regularly to in-country
meetings to foster this exchange of our technologies and practices. In support of this outreach,
we intend to produce at least one fact sheet for each investigation and one overall briefing on our
proposed research to the larger technology transfer community.
Keeping the Mission in Nepal, as well as other offices of USAID, informed of our work and
results is another important outreach activity. We have already contacted Evan Meyer at the
Mission about our planned research and will keep the Mission informed through Evan as we
move to actual field work and workshops. We will ask the Mission for feedback on site selection
for our work, as well as targets for future work plans. We will also keep them informed of our
results through at least annual meetings with the Mission, as well as invitations sent to Mission
officers to attend our workshops and training activities.
Finally, in the research field, it is important to provide information to the research community to
better inform them on possible improvements in aquaculture systems. We have an extensive
history of publishing materials under the previous CRSP in peer-reviewed journals, trade
journals such as the Global Aquaculture Advocate, and books. This extension is equally
important in the development of new knowledge, and we intend to maintain this focus on
research publication. We have also made a special effort to present early results of our work at
local, regional, and international meetings on aquaculture and have been especially conscious of
involving all of our host country personnel in these presentations. As one past example, we
helped co-sponsor the cage culture symposium held in Hangzhou, China in 2006, and a number
of presentations were made there by U.S. and host country personnel. For the present proposal,
we have included financial support for every one of the scientists involved to travel to
international meetings, as well as to conferences within Nepal, to present their results and reach
Diana; Environmentally sustainable aquaculture systems for Nepal Page 25 out to our professional colleagues. As deliverables in this area, we will target the presentation of
research results to regional and international research communities through presentations at local
fisheries meetings, the Asian Fisheries Society and the World Aquaculture Society.
G E N D E R I N C L USI V I T Y ST R A T E G Y
Our proposed work in Nepal includes a variety of cultures and is conducted with a variety of
collaborators. Women and children are the target of much of our research described earlier, and
their nutrition is our major goal in this proposal. We intend to include the participation of women
in our outreach by a number of appropriately targeted activities, including:
1. Producing outreach materials that can be understood by a broad spectrum of society in
Nepal, and focusing on the issue that many residents are illiterate and therefore written
communication is not likely to be successful;
2. Developing at least four ZRPHQ¶VILVKIDUPLQJJURXSVWRGHYHORSLQ-person outreach on
household ponds and other aquaculture methodologies; and
3. Insuring that 50% of our workshop attendees are female.
Women play an important role in aquaculture and in nutrition of poor families. We are
conducting research targeted at this segment of the population and hope to encourage women to
further their part in aquaculture development. For the on-farm trials in Nepal, we will closely
work with an NGO (Rural Integrated Development Society-Nepal), which has been promoting
Women in Aquaculture for many years. As a result, many farmers involved in the on-farm trials
will be women.
We will also hold many workshops during this project, and we will invite 50% women
participants. Women may have difficulties attending workshops due to their responsibilities in
the home, so it is important to do more than just invite them to attend. We will work with our
collaborators to identify these barriers and make appropriate plans to overcome them in our
workshops. Such changes may include the location of workshops, the length of time involved,
and special arrangements developed to allow female participation.
Another objective for gender inclusivity is to involve women in all aspects of our research. This
may be difficult in Nepal because the pipeline at present is largely composed of male scientists,
and for that matter, it has relatively few trained professionals in the fisheries and aquaculture
area of either gender. We are quite proud of the fact that the one female Ph.D. level scientist in
aquaculture (Sunila Rai) was trained under funding from the previous CRSP project. To address
this pipeline, we are targeting that half of the students involved in the project be female, and the
list of students identified so far (see table below) is 50% female. We are providing a number of
graduate fellowships, including tuition costs, to Nepalese students, and will focus these
opportunities on female participants as much as possible. We will work to identify additional
women scientists at each institution involved in the project and to include them in future research
plans, especially at the level of HC Investigator.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 26 Name
James S. Diana
Madhav K. Shrestha
Sunila Rai
Narayan P. Pandit
Dilip K. Jha
Jay Dev Bista
Ramananda Mishra
Bailey Keeler
Ram Bhajan Mandal
Nelson Pokhrel
Laxmi Karki
Sumitra Laudari
Rim Bahadur Thapa
Prashant Sapkota
Md. Saddam Hussen
Institution Gender
Nationality
Principal Investigators
UM
Male
USA
AFU
Male
Nepal
AFU
Female
Nepal
AFU
Male
Nepal
AFU
Male
Nepal
NARC
Male
Nepal
DoFD
Male
Nepal
Students
UM
Female
USA
AFU
Male
Nepal
AFU
Female
Nepal
AFU
Female
Nepal
AFU
Female
Nepal
AFU
Male
Nepal
AFU
Male
Nepal
AFU
Male
Nepal
Diana; Environmentally sustainable aquaculture systems for Nepal Role
US PI
HC PI
HC Investigator
HC Investigator
HC Investigator
HC Investigator
HC Investigator
GSRA
GSRA (PhD)
GSRA
GSRA
GSRA
GSRA
GSRA
GSRA
Page 27 PR O J E C T L E V E L M O N I T O R I N G A N D E V A L U A T I O N P L A N
Diana; Environmentally sustainable aquaculture systems for Nepal Page 28 We will monitor our success and the impact of our project through our investigations, production
of deliverables committed in the various investigations, involvement with professional societies
and local meetings, and estimation of the Feed the Future Indicators. Our pre-estimate for the
FTFMS targets are included on the previous page.
The AquaFish Innovation Lab (AFIL) is focused on development activities that will make a
difference in the life or work of end users such as farmers, host country personnel (including
decision makers, researchers, and extension agents), and USAID missions. We intend to report
our progress through regular completion of quarterly and annual reports, involvement in all AFIL
meetings, and attendance at local and regional meetings of professionals or industry groups. In
addition, we will produce seven workshops and other briefing materials that will spread this
information to end users. We will monitor the success of these activities in a number of ways,
including:
1. Careful tracking of the number and size of farms involved in on farm experiments, as
well as follow up on their continued use of our new technologies;
2. Maintaining good records of attendance at our workshops and other public events,
particularly paying attention to the gender and age of participants;
3. Follow-up surveys with attendees to determine if they have modified their practices as a
result of our workshops;
4. Measuring the advantages of our new technologies in terms of improved yield and profit
in biological and economic units;
5. Surveying 200 members of end-user groups for their fish consumption patterns and the
DGYDQWDJHV LQFUHDVHG ILVK FRQVXPSWLRQ KDV SURGXFHG LQ WHUPV RI FKLOG DQG ZRPHQ¶V
health;
6. Following up on the results of our four school ponds and four ZRPHQ¶V ILVK IDUPLQJ
groups in terms of the number of people who have adopted aquaculture systems as a
result of these projects; and
7. Publishing our results in peer-reviewed journals, as well as practitioner journals, and
keeping track of their impact by use of citation rates.
8. We have already initiated contact with the USAID Mission in Nepal (Evan Meyer) and
intend to involve them in investigation planning, as well as inform them of progress
through meetings on at least an annual basis.
9. We have also initiated contact with Winrock International and the Nutrition CRSP to
secure their help in focusing our work on nutrition, as well as to utilize their networks to
better disseminate and measure the impact of our projects by NGOs and government
agencies.
10. We have also involved government agencies, mainly NARC, in the conduct of our
investigations and our plans for dissemination. This will better insure the dissemination
of results to government officials, as well as the ability to determine how many officials
have been informed of these studies.
These activities will give us an accurate and detailed estimate of the impacts of our work and
will be reported through AFIL methodologies to the management entity as well as USAID.
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Pandit, N.P., M.K. Shrestha, Y. Yi, and J.S. Diana. 2004. Polyculture of grass carp and Nile tilapia
with napier grass as the sole nutrient input in the subtropical climate of Nepal. In: R.
Boliver, G. Mair, and K. Fitzsimons (eds.), New Dimension in Farmed tilapia, Proceedings
of 6th International Symposium on Tilapia in Aquaculture (ISTA 6), pp. 558-573.
Pantha, M.B. 1993. Aquafeeds and feeding strategy in Nepal. In: M.W. New, A.G.T. Tacon, and
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Nepal, an area of micronutrient deficiency. Journal of Biosocial Science 44:401-415.
Paudel, J.K., D.K. Jha, M.K. Shrestha, and J.D. Bista. 2007. Growth performance of Sahar (Tor
putitora) in different culture systems in Chitwan, Nepal. In: R.B. Thapa and M.D. Sharma
(eds.), IAAS Research Advances, Volume 2. Institute of Agriculture and Animal Science,
Rampur, Chitwan, Nepal, pp. 195-200.
Prasad, M.S., A.P. Mishra, and B.R. Singh. 1993. Induced breeding, larval behaviour and
development of the air breathing habit in some freshwater teleosts. In: B.R. Singh (ed.).
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Rai, A.K. 2008. Status of sahar (Tor putitora) domestication and its development in the
Himalayan Region of Nepal. Aquaculture Asia Magazine, Jan-Mar 2008: 26-32.
5DL 6 3URMHFW &RPSOHWLRQ 5HSRUW ,PSURYHPHQW RI ZRPHQ¶V OLYHOLKRRGV LQFRPH DQG
nutrition through Carp-SIS polyculture in Terai, Nepal. Institute of Agriculture and
Animal Science, Chitwan, Nepal.
5DL 6 4XDUWHUO\ 5HSRUW 7ZLQQLQJ 6XSSRUW WR :RPHQ )LVK )DUPHUV¶ 2UJDQL]DWLRQV LQ
Nepal. Institute of Agriculture and Animal Science, Chitwan, Nepal.
Rai S., M.K. Shrestha, D.K. Jha, and D. Acharya. 2007. Growth performance of Sahar and mixedsex Nile tilapia in monoculture and co-culture system in Chitwan. In: R.B. Thapa and M.D.
Sharma (eds.), IAAS Research Advances, Volume 1. Institute of Agriculture and Animal
Science, Rampur, Chitwan, Nepal, pp. 187-193.
Rai, S., and Y. Yi. 2012. Nibbling frequency of carps in Periphyton-Based Aquaculture Systems
with and without Supplemental Feed. Israeli Journal of Aquaculture 64:818-822.
Rai, S., Y. Yi, M.A. Wahab, A. Bart, and J.S. Diana. 2008. Comparison of rice straw and
bamboo sticks substrates in periphyton-based carp polyculture systems. Aquaculture
Research 39:464-473.
Rajbanshi, K.G. 2001. Zoo-geographical distribution and the status of cold water fishes of Nepal.
Symposium on coldwater fishes in the trans Himalayan region, 10-14 July, Kathmandu,
Nepal.
Rana, P.J.B., and K.G. Rajbanshi. 1976. National Plan for development of aquaculture in Nepal.
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agriculture and health. International Food Policy Research Institute, Washington, D.C.
Roos, N., M.A. Wahab, M.A.R. Hossain, and S.H. Thilsted. 2007. Linking human nutrition and
fisheries: incorporating micro-nutrient dense, small indigenous fish species in carp
polyculture in Bangladesh. Food and Nutrition Bulletin.
Saxena, P.K., and R. Sandhu. 1994. Influence of temperature on ovarian recrudescence in the
Indian catfish, Heteropneustes fossilis. Journal of Fish Biology 44:168-171.
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with mixed-sex Nile tilapia. Aquaculture 319:284-289.
Shrestha, J. 1994. Fishes, fishing implements and methods of Nepal. Published by Smt. M.D.
Gupta, Lalitpur Colony, Lashkar (Gwalior), India, pp. 144.
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strategies. Bimala Shrestha, Kathmandu, Nepal, pp. 259.
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polyculture with common carp ( Cyprinus carpio) fed with duckweed (Spirodela) in Nepal.
Asian Fisheries Science 12:83-89.
Shrestha, M.K., J.D. Bista, and Y. Yi. 2007. Performance of Tor putitora in sub-tropical climate of
Nepal. 8th Asian Fisheries Forum. Asian Fisheries Society and Asian Fisheries Society,
Indian Branch, 20 - 23 November 2007, Kochi, India. Fisheries and Aquaculture: Strategic
Outlook for Asia, Book of Abstracts, pp. 250-251.
Shrestha, M.K., R. Jaiswal, L. Liping, and J.S. Diana. 2012. Incorporation of tilapia ( Oreochromis
niloticus) and sahar (Tor putitora) into the existing carp polyculture system in Nepal.
AquaFish CRSP Technical Reports Investigations 2009-2011, Volume 1, August 2012,
Oregon State University, Corvalis.
Shrestha, M.K., N.P. Pandit, Y. Yi, J.S. Diana, and C.K. Lin. 2005. Integrated cage-cum-pond
culture system with high-valued Sahar (Tor putitora ) suspended in carp polyculture
ponds. In: J. Burright, C. Flemming, and H. Egna (eds.), Twenty-Second Annual
Technical Report. Aquaculture CRSP, Oregon State University, Corvallies, Oregon, pp.
97-114.
Shrestha, M.K., J. Pant, R.C. Bhujel. 2012. Small-scale aquaculture development model for rural
Nepal. In: M.K. Shrestha and J. Pant (eds.) Small-scale Aquaculture for Rural
Livelihoods: Proceedings of the National Symposium on Small-scale Aquaculture for
Increasing Resilience of Rural Livelihoods in Nepal. Institute of Agriculture and Animal
Science, Tribhuvan University, Rampur, Chitwan, Nepal, and The WorldFish Center,
Penang, Malaysia, pp. 71-75.
Shrestha, M.K., R.L. Sharma, K. Gharti, and J.S. Diana. 2011. Polyculture of sahar ( Tor
putitora ) with mixed-sex Nile tilapia. Aquaculture 319:284-289.
Sinha V.R.P. 1993. A compendium of aquaculture technologies for developing countries. Oxford
and IBH Publishing Co. Pvt. Ltd. New Delhi. 110001, pp. 115.
Speedy, A.W. 2003. Global Production and Consumption of Animal Source Foods. Journal of
Nutrition 133:4048S-4053S.
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University of Michigan, Ann Arbor (in process).
Diana; Environmentally sustainable aquaculture systems for Nepal Page 33 Stepan, Z., M. Shrestha, and J.S. Diana. 2013. A value chain analysis for carp produced by
polyculture in farms of the Chitwan area, Nepal. Final Investigation Report
09MER11UM, AquaFish CRSP, Corvallis, OR (accessed 29 April 2013 at
http://aquafishcrsp.oregonstate.edu/UM/).
Tharakan, B., and K.P. Joy. 1996. Effects of mammalian gonadotropin-releasing hormone
analogue, Pimozide, and the combination on plasma gonadotropin levels in different
seasons and induction of ovulation in female catfish. Journal of Fish Biology 48:623-632.
UNICEF. 2012a. A Milestone Plan Launched to Improve Maternal and Child Nutrition in Nepal.
UNICEF. 2012b. Multi-sectoral Commitment to Improve Maternal and Child Nutrition.
Wahab, M.A., M.A. Alim, and A. Milstein. 2003. Effects of adding the small fish Punti ( Puntius
Sophore Hamilton) and/or Mola ( Amblypharyngodon Mola Hamilton) to a polyculture of
large carp. Aquaculture Research 2003:149-63.
Yadav, C.N.R. 1999. Artificial and semi-artificial propagation of Asian catfish, (Bloch). Thesis
No. AS. 99-13. Asian Institute of Technology. Bangkok, Thailand, pp. 68.
Yadav, C.N.R., and A.N. Bart. 2003. Hatchery breeding of catfish, Heteropneustes fossilis
(Bloch) in Nepal. Journal of the Institute of Agriculture and Animal Science 24:59-66.
Yadav, R.K., M.K. Shrestha, and N.P. Pandit. 2007. Introduction of Sahar (Tor putitora) in cagecum-pond integrated system of mixed-sex Nile tilapia ( Oreochromis niloticus). Our Nature
5:52-59.
Yi, Y., J.S. Diana, M.K. Shrestha, and C. K. Lin. 2004. Culture of mixed-sex Nile tilapia with
predatory snakehead. In: R. Boliver, G. Mair, and K. Fitzsimons (eds.) New Dimension in
Farmed tilapia, Proceedings of 6th International Symposium on Tilapia in Aquaculture
(ISTA 6), pp. 544-557.
Diana; Environmentally sustainable aquaculture systems for Nepal Page 34 H OST C O U N T R Y R ESE A R C H L O C A T I O N
This proposal involves the country of Nepal, which is currently among the countries of the
AquaFish CRSP. It is also one of the countries targeted by the F eed the Future program of
USAID and requires no additional justification by standards outlined in the RFP. The main host
country institution is the Agriculture and Forestry University, formerly known as the Institute for
Agriculture and Animal Science, which was part of Tribhuvan University. It has recently
separated from that institution, so we will need to develop a new Memorandum of Understanding
between UM and AFU. In addition, AFU will subcontract with the Nepal Agriculture Research
Center, so another MOU will be necessary between those two institutions.
4.
BUDG E T
Diana; Environmentally sustainable aquaculture systems for Nepal Page 35 BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner I nstitution Proposal Budget
Proposal Title
Institution
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
Lead Principal Investigator
University of Michigan
Year 1
Budget Period:
James Diana
Budget Categories
From (mm/dd/yy):
7/1/2013
To (mm/dd/yy):
9/30/2013
US Cost-share
HC/Regional Cost
Contribution
US Cost
Personnel Costs
Salary
Benefits/Fringe
Subtotal
$
$
$
-
$
-
$
-
$
-
$
-
$
-
$
-
$
-
Travel
Domestic
International
Subtotal
$
$
-
$
$
$
-
Equipment
Tuition
$
-
$
-
Total Direct Cost
$
-
$
-
Indirect Costs
Standard Indirect
Indirect on Subcontracts
Subtotal
$
$
$
-
$
-
$
-
$
-
$
-
$
-
$
-
Total
$
-
$
-
$
-
$
-
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1
Subcontract to US Inst #2
Subcontract to HC Inst #1
Subcontract to HC Inst #2
Subtotal
$
$
$
-
HC M atching
Contribution
Additional US Non-Federal Cost
Share from US Inst #1
Additional US Non-Federal Cost
Share from US Inst #2
GRAND TOTAL
$
-
$
-
$
-
$
-
BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner I nstitution Proposal Budget
Proposal Title
Institution
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
Lead Principal Investigator
University of Michigan
Year 2
Budget Period:
James Diana
Budget Categories
Personnel Costs
Salary
Benefits/Fringe
Subtotal
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1
Subcontract to US Inst #2
Subcontract to HC Inst #1
Subcontract to HC Inst #2
Subtotal
From (mm/dd/yy):
10/1/2013
To (mm/dd/yy):
9/30/2014
US Cost-share
HC/Regional Cost
Contribution
US Cost
$
$
$
38,781 $
8,617 $
47,398 $
$
5,000
$
124,310
$
Travel
Domestic
International
Subtotal
26,762 $
4,930
31,692 $
HC M atching
Contribution
24,600
24,600
$
47,278 $
129,310
$
47,278
$
$
$
1,500
8,500
10,000
$
$
$
5,000
26,000
31,000
Equipment
Tuition
$
10,200
$
6,000
Total Direct Cost
$
196,908
$
108,878
Indirect Costs
Standard Indirect
Indirect on Subcontracts
Subtotal
$
$
$
34,631 $
13,875
48,506 $
17,589 $
15,432 $
17,589 $
15,432
Total
$
245,414 $
49,281 $
21,250
3,750
$
124,310 $
25,000
Additional US Non-Federal Cost
Share from US Inst #1
Additional US Non-Federal Cost
Share from US Inst #2
GRAND TOTAL
$
245,414
$
49,281
$
124,310
$
25,000
BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner I nstitution Proposal Budget
Proposal Title
Institution
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
Lead Principal Investigator
University of Michigan
Year 3
Budget Period:
James Diana
Budget Categories
Personnel Costs
Salary
Benefits/Fringe
Subtotal
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1
Subcontract to US Inst #2
Subcontract to HC Inst #1
Subcontract to HC Inst #2
Subtotal
10/1/2014
From (mm/dd/yy):
9/30/2015
To (mm/dd/yy):
US Cost-share
HC/Regional Cost
Contribution
US Cost
$
$
$
49,344 $
11,602 $
60,946 $
27,565 $
5,177
32,742 $
25,800
25,800
$
5,000
$
- $
$
125,690
$
-
$
130,690
$
- $
Travel
Domestic
International
Subtotal
$
$
$
1,500
8,500
10,000
$
$
$
5,000
16,000
21,000
Equipment
Tuition
$
10,608
$
6,000
Total Direct Cost
$
212,244
$
110,078
Indirect Costs
Standard Indirect
Indirect on Subcontracts
Subtotal
Total
$
HC M atching
Contribution
57,278 $
21,250
57,278 $
21,250
18,172 $
15,612 $
3,750
$
42,151 $
42,151 $
18,172 $
15,612 $
3,750
$
254,395 $
50,913 $
125,690 $
25,000
$
Additional US Non-Federal Cost
Share from US Inst #1
Additional US Non-Federal Cost
Share from US Inst #2
GRAND TOTAL
$
254,395
$
50,913
$
125,690
$
25,000
BUDGET FORM
AquaFish Innovation Lab
Combined-Year US Lead Partner I nstitution Proposal Budget
Proposal Title
Institution
University of Michigan
Budget Period:
Combined Years
From (mm/dd/yy):
7/1/2013
To (mm/dd/yy):
9/30/2015
US Cost-share
HC/Regional Cost
Contribution
Lead Principal Investigator
James Diana
Budget Categories
Personnel Costs
Salary
Benefits/Fringe
Subtotal
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1
Subcontract to US Inst #2
Subcontract to HC Inst #1
Subcontract to HC Inst #2
Subtotal
US Cost
$
$
$
88,125
20,219 $
108,344 $
$
10,000
$
250,000
$
Travel
Domestic
International
Subtotal
54,327 $
10,107 $
64,434 $
HC M atching
Contribution
50,400
50,400
$
104,556 $
260,000
$
104,556
$
$
$
3,000
17,000
20,000
$
$
$
10,000
42,000
52,000
Equipment
Tuition
$
20,809
$
12,000
Total Direct Cost
$
409,153 $
64,434 $
218,956
Indirect Costs
Standard Indirect
Indirect on Subcontracts
Subtotal
$
$
$
76,781 $
13,875
90,656 $
35,761 $
31,044 $
35,761 $
31,044
Total
$
499,809 $
100,195 $
250,000 $
42,500
7,500
50,000
Additional US Non-Federal Cost
Share from US Inst #1
Additional US Non-Federal Cost
Share from US Inst #2
GRAND TOTAL
$
499,809 $
100,195 $
250,000 $
50,000
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
HC Principal Investigator
Madhav Shrestha
Budget Categories
HC Institution
Agriculture and Forestry Univ (AFU), Nepal
Budget Period:
Year 1
From (mm/dd/yy): 7/1/2013
To (mm/dd/yy): 9/30/2013
HC Contribution
Host Country Cost
Personnel Costs
Salary
$
-
$
-
$
-
$
-
Domestic
$
-
International
$
-
$
-
Tuition
$
-
Total Direct Cost
$
-
$
-
Subtotal
$
-
Total
$
-
Benefits/Fringe
Subtotal
Supplies and Services
S& S
Subtotal
Travel
Subtotal
Equipment
Indirect Costs
Standard Indirect
$
-
$
-
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
HC Principal Investigator
Madhav Shrestha
Budget Categories
HC Institution
Agriculture and Forestry Univ (AFU), Nepal
Budget Period:
Year 2
From (mm/dd/yy): 10/1/2013
To (mm/dd/yy): 9/30/2014
HC Contribution
Host Country Cost
Personnel Costs
Salary
Benefits/Fringe
Subtotal
$
$
24,600
-
$
24,600
$
47,278 $
$
47,278
Domestic
$
5,000
International
$
26,000
$
31,000
Supplies and Services
S& S
Subtotal
21,250
Travel
Subtotal
Equipment
$
-
Tuition
$
6,000
Total Direct Cost
$
108,878
Indirect Costs
$
15,432 $
Subtotal
$
15,432
Total
$
Standard Indirect
124,310 $
3,750
25,000
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
HC Principal Investigator
Madhav Shrestha
Budget Categories
HC Institution
Agriculture and Forestry Univ (AFU), Nepal
Budget Period:
Year 3
From (mm/dd/yy): 10/1/2014
To (mm/dd/yy): 9/30/2015
HC Contribution
Host Country Cost
Personnel Costs
Salary
Benefits/Fringe
Subtotal
$
$
25,800
-
$
25,800
$
57,278 $
$
57,278
Domestic
$
5,000
International
$
16,000
$
21,000
Supplies and Services
S& S
Subtotal
21,250
Travel
Subtotal
Equipment
$
-
Tuition
$
6,000
Total Direct Cost
$
110,078
Indirect Costs
$
15,612 $
Subtotal
$
15,612
Total
$
Standard Indirect
125,690 $
3,750
25,000
BUDGET FORM
AquaFish Innovation Lab
Combined-Year HOST COUNTRY I nstitution Budget
Proposal Title
Development of more efficient and environmentally
sustainable aquaculture systems for Nepal.
HC Principal Investigator
Madhav Shrestha
Budget Categories
HC Institution
Agriculture and Forestry Univ (AFU), Nepal
Budget Period:
Combined Years
From (mm/dd/yy): 7/1/2013
To (mm/dd/yy): 9/30/2015
HC Contribution
Host Country Cost
Personnel Costs
Salary
Benefits/Fringe
Subtotal
$
$
$
50,400
50,400
Supplies and Services
$
104,556 $
$
104,556
Domestic
$
10,000
International
$
42,000
$
52,000
S& S
Subtotal
42,500
Travel
Subtotal
Equipment
$
-
Tuition
$
12,000
Total Direct Cost
$
218,956
Indirect Costs
Standard Indirect
$
31,044 $
7,500
$
250,000 $
50,000
Subtotal
Total
BUDGET JUSTIFICATION FORM FOR US LEAD PARTNER INSTITUTIONS
Year 1 (3 months)
No funds requested in year one.
Year 2
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name
James
Diana
Bailey
Keeler
unknown
Title
Annual
Salary
Benefits
Rate
Benefits
$145,096
19%
FTE
0
Salary
Professor
GSRA
$28,172
15%
.66
$18,781
$2,817
Research
Assistant
$50,000
29%
.4
$20,000
$5,800
$38,781
$8,617
Total
Project Funds
Cost Share Funds
FTE
0.19
Salary
$26,762
Benefits
$4,930
$26,762
$4,930
1b. Student Information (you will be asked to complete a student profile form upon award)
Name
Gender
Nationality
Degree Sought
Bailey Keeler
Male
USA
MS
1c. Cost Share
US non-­federal cost share is $49,282, which is at least 20% of the total.
Salary – 1.66 months of James Diana Academic Year -­ $26,762
Fringe & Benefits – 19% of total salary -­ $4,930
Indirect Cost of 55.5% on total salary & benefits -­ $17,589
Total: $49,282
2. Services and Supplies
Laboratory and field supplies, including chemicals, aquaculture inputs, and fish: $5,000
Subcontract to HC Institution #1 -­ $124,310
3. Travel
Domestic Travel
x Date
September 2013 Name J. Diana
Reason for travel: startup meeting
x Departure Location: Detroit
Arrival Location:
Washington DC
x Meals and Lodging @ $ 300 /day for 4 days
$1,200
x Airfare and ground transportation
$300
x Registration
$0
x TOTAL
$1,500
International Travel
x Date
March 2014
Name
GSRA Reason for travel: research in Nepal
x Departure Location: Detroit
Arrival Location: Kathmandu
x Meals and Lodging @ $ 30/day for 30 days
$900
Page 1 of 4
x
x
x
x
Airfare
Airport transport, parking, etc.
Miscellaneous (departure tax, etc.)
TOTAL
$2,000
$100
$
$3,000
International Travel
x Date
May 2014
Name J. Diana
x Departure Location: Detroit
x Meals and Lodging @ $ 40/day for 10 days
x Airfare
x Airport transport, parking, etc.
x Miscellaneous (departure tax, etc.)
x TOTAL
Reason for travel: research in Nepal
Arrival Location: Kathmandu
$400
$2,000
$100
$
$2,500
International Travel
x Date
August 2014
Name
GSRA Reason for travel: research in Nepal
x Departure Location: Detroit
Arrival Location: Kathmandu
x Meals and Lodging @ $ 30/day for 30 days
$900
x Airfare
$2,000
x Airport transport, parking, etc.
$100
x Miscellaneous (departure tax, etc.)
$
x TOTAL
$3,000
4. Tuition
One semester for GSRA at Pre-­candidate rate: $10,200
5. Indirect Costs
Total Direct Cost: $196,908
<LESS> Tuition (not subject to indirect): $10,200
<LESS> Subcontract to HC Institution #1: $124,310
TOTAL Direct Subject to Standard Indirect: $62,398
Rate of Regular Indirect
55.5%
On-­/Off-­campus
On campus
Amount
$34,631
Rate of Indirect on
Subcontracts
Amount per
Subcontract
Number of
Subcontracts
Total Amount
55.5% of First $25,000 of
each subcontract
$124,310 (HC,
Nepal)
1
$13,875
6.
HC/Regional Cost
Subcontract to HC Institution #1 -­ $124, 310
TOTAL: $124, 310
Page 2 of 4
Year 3
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name
Title
Annual
Salary
Benefits
Rate
J Diana
unknown
unknown
Professor
GSRA-­UM
Research
Assistant
$149,449
$29,017
$50,000
19%
15%
29%
Project Funds
FTE
0
.66
.6
Total
Salary
Benefits
$19,344
$30,000
$2,902
$8,700
$49,344
$11,602
Cost Share Funds
FTE
0.19
Salary
$27,565
Benefits
$5,177
$27,565
$5,177
1b. Student Information (you will be asked to complete a student profile form upon award)
Name
Gender
Nationality
Degree Sought
Bailey Keeler
Female
USA
MS
1c. Cost Share
US non-­federal cost share is $50,913 which is at least 20% of the total.
Salary – 1.66 months James Diana Academic Year -­ $27,565
Fringe & Benefits – 19% of total salary -­ $5,177
Indirect Cost of 55.5% on total salary & benefits -­ $18,172
2. Services and Supplies
Laboratory and field supplies, including chemicals, aquaculture inputs, and fish $5,000
Subcontract HC (Nepal): $125,690
3. Travel
Domestic Travel
x Date
October 2014 Name J. Diana
Reason for travel: annual meeting
x Departure Location: Detroit
Arrival Location:
unknown
x Meals and Lodging @ $ 200 /day for 4 days
$800
x Airfare
$600
x Airport transport, parking, etc.
$100
x Registration
$0
x TOTAL
$1,500
International Travel
x Date
March 2015
Name
GSRA Reason for travel: research in Nepal
x Departure Location: Detroit
Arrival Location: Kathmandu
x Meals and Lodging @ $ 30/day for 30 days
$900
x Airfare
$2,000
x Airport transport, parking, etc.
$100
x Miscellaneous (departure tax, etc.)
$
x TOTAL
$3,000
Page 3 of 4
International Travel
x Date
May 2015
Name J. Diana
x Departure Location: Detroit
x Meals and Lodging @ $ 40/day for 10 days
x Airfare
x Airport transport, parking, etc.
x Miscellaneous (departure tax, etc.)
x TOTAL
Reason for travel: research in Nepal
Arrival Location: Kathmandu
$400
$2,000
$100
$0
$2,500
International Travel
x Date
August 2015
Name
GSRA Reason for travel: research in Nepal
x Departure Location: Detroit
Arrival Location: Kathmandu
x Meals and Lodging @ $ 30/day for 30 days
$900
x Airfare
$2,000
x Airport transport, parking, etc.
$100
x Miscellaneous (departure tax, etc.)
$0
x TOTAL
$3,000
4. Tuition
One semester for GSRA at pre-­candidate rate: $10,608
5. Indirect Costs
Total Direct Cost: $212,244
<LESS> Tuition (not subject to indirect): $10,608
<LESS> Subcontract to HC Institution #1: $125,690
TOTAL Direct Subject to Standard Indirect: $75,946
Rate of Regular Indirect
55.5%
Rate of Indirect on
Subcontracts
55.5% of First $25,000 of
each subcontract
6.
On /off campus
On campus
Amount per
Subcontract
$125,690 (HC,
Nepal)
Number of
Subcontracts
1
Amount
$42,150
Total Amount
$0
HC/Regional Cost
Subcontract to HC Institution #1 -­ $125, 690
TOTAL: $125, 690
Page 4 of 4
AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS AND FORM
FOR HOST COUNTRY INSTITUTIONS
Year 1
No funds requested in year 1.
Year 2
1. Personnel, Benefits and US Cost Share
1a. Salary Information
Name
Madhav
Shrestha
Sunila Rai
DK Jha
CNR
Yadav
AK Singh
JD Bista
NP Pandit
R Mishra
See the list
below (1)
See the list
below(5)
Unknown
(10)
Unknown
Unknown
Title
Annual
Salary
Benefits
Rate
Project Funds
Cost Share Funds
Professor
$5,850
0%
FTE
.41
Salary
$2,400
Benefits
$0
lecturer
lecturer
lecturer
$3,900
$3,900
$3,900
0%
0%
0%
.31
.31
.31
$1,200
$1,200
$1,200
$0
$0
$0
lecturer
lecturer
lecturer
lecturer
GSRA-­
PhD
Nepal
GSRA-­MS
Nepal
UGRA-­
Nepal
Farm
Manager
Research
Assistant
$3,900
$4,550
$3,250
$3,900
$1,200
0%
0%
0%
0%
0%
.19
.27
.0
.31
1.0
$750
$1,200
$0
$1,200
$1,200
$0
$0
$0
$0
$0
$900
0%
5.0
$4,500
$0
$375
0%
10.0
$3,750
$0
$3,000
0%
1.0
$3,000
$0
$3,000
0%
1.0
$3,000
$0
$24,600
$0
Total
FTE
Salary
Benefits
1b. Student Information (you will be asked to complete a student profile form upon award)
Name
Ram B Mandal
Nelson Pokhrel
Laxmi Karki
Sumitra Laudari
Rim B Thapa
Prashant Sapkota
Md S Hussen
Gender
Male
Female
Female
Female
Male
Male
Male
Nationality
Nepal
Nepal
Nepal
Nepal
Nepal
Nepal
Nepal
Degree Sought
PhD
MS
MS
MS
MS
MS
MS
1c. Cost Share
HC match is $25,000 in year one for providing Office space, Lab facilities and pond facilities.
2.
Services and Supplies
Lab chemicals -­ $5,000
Page 1 of 4
Water Quality Lab Set Up -­ $17,500
Farm Pond Development -­ $20,000
Computer (4) -­ $2,778
Journal (2) -­ $2,000
3.
Travel
International Travel
x Date
September 2013
Name M. Shrestha
Reason for travel: Startup
meeting
x Departure Location: Detroit
Arrival Location: Washington, DC;; Ann Arbor;; Corvallis
x Meals and Lodging @ $ 200 /day for 20 days
$4,000
x Airfare
$1,950
x Airport transport, parking, etc.
$50
x Registration
$0
x TOTAL
$6,000
4.
International Travel
x Date
May 2014
Name 5 people
x Departure Location: Kathmandu
x Meals and Lodging @ $ 300/day for 5 days
x Airfare
x Airport transport, parking, etc.
x Miscellaneous (departure tax, etc.)
x Each
x TOTAL
Reason for travel: annual meeting
Arrival Location: uncertain
$1,500
$2,450
$50
$0
$4,000
$20,000
Domestic Travel
x Date
all year
Name 5 people
x Departure Location: Rampur
x Meals and Lodging @ $ 30/day for 30 days
x Vehicle/Ground transport
x Miscellaneous (departure tax, etc.)
x Each
x TOTAL
Reason for travel: research meetings
Arrival Location: uncertain
$900
$100
$0
$1,000
$5,000
Tuition
PhD student (1) $2,000
MSc student (5) $3,000
BSc student (10) $1,000
5.
Indirect Costs
Total Direct Cost: $108,878
<LESS> Tuition (not subject to indirect): $6,000
TOTAL Direct Subject to Standard Indirect: $102,878
Rate of Regular Indirect
On-­/Off-­campus
15%
On campus
6.
Amount
$15,432
INFORMATIONAL ONLY: For HC/Regional Costs that are included in the Lead Budget
Page 2 of 4
N/A
Year 3
1.
Personnel, Benefits and US Cost Share
1a. Salary Information
Name
Madhav
Shrestha
Sunila Rai
DK Jha
CNR
Yadav
AK Singh
JD Bista
NP Pandit
R Mishra
See the list
below (1)
See the list
below(5)
Unknown
(10)
Unknown
Unknown
Title
Annual
Salary
Benefits
Rate
Project Funds
Cost Share Funds
Professor
$5,850
0%
FTE
.41
Salary
$2,400
Benefits
$0
lecturer
lecturer
lecturer
$3,900
$3,900
$3,900
0%
0%
0%
.31
.31
.31
$1,200
$1,200
$1,200
$0
$0
$0
lecturer
lecturer
lecturer
lecturer
GSRA-­
PhD
Nepal
GSRA-­MS
Nepal
UGRA-­
Nepal
Farm
Manager
Research
Assistant
$3,900
$4,550
$3,250
$3,900
$1,200
0%
0%
0%
0%
0%
.19
.27
.37
.31
1.0
$750
$1,200
$1,200
$1,200
$1,200
$0
$0
$0
$0
$0
$900
0%
5.0
$4,500
$0
$375
0%
10.0
$3,750
$0
$3,000
0%
1.0
$3,000
$0
$3,000
0%
1.0
$3,000
$0
$25,800
$0
Total
FTE
Salary
Benefits
1b. Student Information (you will be asked to complete a student profile form upon award)
Name
Ram B Mandal
Nelson Pokhrel
Laxmi Karki
Sumitra Laudari
Rim B Thapa
Prashant Sapkota
Md S Hussen
Gender
Male
Female
Female
Female
Male
Male
Male
Nationality
Nepal
Nepal
Nepal
Nepal
Nepal
Nepal
Nepal
Degree Sought
PhD
MS
MS
MS
MS
MS
MS
1c. Cost Share
HC match is $25,000 in year two for providing Office space, Lab facilities and pond facilities.
2.
Services and Supplies
Lab chemicals -­ $5,000
Water Quality Lab Set Up -­ $17,500
Farm Pond Development -­ $30,000
Computer (4) -­ $2,778
Journal (2) -­ $2,000
3.
Travel
Page 3 of 4
International Travel
x Date
May 2015
Name 4 people
x Departure Location: Kathmandu
x Meals and Lodging @ $ 300/day for 5 days
x Airfare
x Airport transport, parking, etc.
x Miscellaneous (departure tax, etc.)
x Each
x TOTAL
Domestic Travel
x Date
all year
Name 5 people
x Departure Location: Rampur
x Meals and Lodging @ $ 30/day for 30 days
x Vehicle/Ground Transport
x Miscellaneous (departure tax, etc.)
x Each
x TOTAL
4.
Reason for travel: annual meeting
Arrival Location: uncertain
$1,500
$2,500
$0
$0
$4,000
$16,000
Reason for travel: research meetings
Arrival Location: uncertain
$900
$100
$1,000
$5,000
Tuition
PhD student (1) $2,000
MSc student (5) $3,000
BSc student (10) $1,000
5.
Indirect Costs
Total Direct Cost: $110,078
<LESS> Tuition (not subject to indirect): $6,000
TOTAL Direct Subject to Standard Indirect: $104,078
Rate of Regular Indirect
15%
6.
On-­/Off-­campus
On campus
Amount
$15,612
INFORMATIONAL ONLY: For HC/Regional Costs that are included in the Lead Budget
N/A
Page 4 of 4
6. P R O P OSA L L E V E R A G I N G S T A T E M E N T
AND
P E N D I N G F U N DS D ISC L OSU R E F O R M
L E V E R A G E D SUPPO R T
L E V E R A G I N G A C T I V I T I ES T O D A T E U N D E R Y O U R C U R R E N T PR O J E C T
Under the current AquaFish CRSP, we were able to leverage $100,000 from the University
of Michigan (Graham Environmental Sustainability Institute) for research on the
sustainability of shrimp aquaculture systems. This project funded research on life cycle
assessment of shrimp culture in Michigan, as well as microbial analysis of Recirculating
Aquaculture systems for shrimp.
We also received another $200,000 from the National Science Foundation to evaluate RAS
systems for shrimp, including a life cycle assessment of domestic produced compared to
imported shrimp from China.
A N T I C IP A T E D L E V E R A G I N G F R O M P R O P OSE D W O R K
We have been successful in leveraging resources in the past to help with our work in aquaculture
and anticipate that leveraging will be available in the future. At present, we have no clear paths
for this leveraging, but we have made contact with two other research groups (Winrock
International and the Nutrition CRSP, two other NGOs concerned with human nutrition in
Nepal). We anticipate that they will at least aid us in designing survey tools to our nutrition
surveys, and we hope they will conduct similar surveys in other regions or for other types of
consumers to gain an even larger database on consumption and nutrition in Nepal.
We also continue to work in the area of sustainable aquaculture with colleagues from the College
of Engineering, School of Public Health, and the Center for Sustainable Systems at University of
Michigan. We are in the process of proposing research projects with these colleagues, and the
existence of the AquaFish Innovation Lab project will provide leverage for these proposals, as
well as allow the coordination of work between these groups and the enhancing of work done by
each group.
P E N D I N G F U N DS
Disclosure Information Provided for: James Diana
Proposal Title
and Lead PI:
Development of more efficient and environmentally sustainable aquaculture systems for
Nepal.
James S. Diana
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
James S. Diana
None
C ur rent/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for nonAquaFish funding.
Project Title: Sea Grant Aquaculture Extension 2013: Developing Business Clusters to Vertically
Integrate New Aquaculture Development in Michigan
Project Status:
( ) Current ( x ) Pending
Funding Agency: NOAA
Total Award Amount: $300,000
Location of Project: Ann Arbor
( ) Submission Planned in near Future
Total Award Period: 19/1/13 to 8/31/15
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Diana 0.08
Project Title: Improving Sustainability and Reducing Environmental Impacts of Aquaculture in Southeast
Asia
Project Status:
( x ) Current ( ) Pending
( ) Submission Planned in near Future
Funding Agency: US Agency for International Development
Total Award Amount: $828,698
Total Award Period: 11/6/07 to 6/30/13
Location of Project: Ann Arbor and Southeast Asia
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Diana: 0.3 FTE
Project Title: Exploring changes in energy transfer within Great Lakes food webs
Project Status:
( x ) Current ( ) Pending
( ) Submission Planned in near Future
Funding Agency: US Department of Interior
Total Award Amount: $76,198
Total Award Period: 8/8/11 to 12/13/13
Location of Project: Ann Arbor
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Diana: 0 FTE
Project Title: International Management of Fisheries in Large Lakes
Project Status:
( x ) Current ( ) Pending
( ) Submission Planned in near Future
Funding Agency: Great Lakes Fishery Commission
Total Award Amount: $372,565 Total Award Period: 1/3/08 to 1/2/14
Location of Project: Ann Arbor
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Diana: 0 FTE
Project Title: Michigan Sea Grant Omnibus
Project Status:
( x ) Current ( ) Pending
( ) Submission Planned in near Future
Funding Agency: NOAA
Total Award Amount: $4,089,746
Total Award Period: 2/1/10 to 1/31/14
Location of Project: Ann Arbor
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Diana: 0.5 FTE
Madhav Shrestha
Current/Pending Research Projects
Project Title: Integration of small indigenous fish species (SIS) in carp polyculture system
Project Status:
( x ) Current ( ) Pending
( ) Submission Planned in near Future
Funding Agency: WorldFish
Total Award Amount: US$ 22,000
Total Award Period: One Year
Location of Project: Rupendehi and Nawalparasi, Nepal
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.08 FTE
7. LETTERS OF COMMITMENT FROM ALL PERSONNEL
8.
9. LEAD INSTITUTION SUPPORTING INFORMATION
ANIMAL USE APPROVAL
The University Committee on Use and Care of Animals has reviewed your renewal application
to use vertebrate animals referenced below. The project has been approved. The proposed animal
use procedures are in compliance with University guidelines, State and Federal regulations, and
the standards of the "Guide for the Care and Use of Laboratory Animals."
There may be additional issues that need to be addressed prior to initiation of the associated
research. It is your responsibility, as Principal Investigator, to secure all the necessary
requirements and recommendations (e.g., IBC approval, Animal Use training, accepting OSEH
Findings and Recommendations).
A formal letter of approval for each fund source and title will follow.
Principal Investigator: James Diana
Protocol: PRO00004861
Protocol Title: Collaborative Research Support Program
Approval Period: 4/19/2013 - 4/19/2016
Please note the following information for this renewal:
Only procedures stated in your renewal may now be performed.
You will be contacted to transfer your animals, if any, from your expiring protocol 08825-3 to
the renewed protocol PRO00004861.
Sincerely,
Dr. Keith Cook, Ph.D.
Professor and Chairperson
University Committee on Use and Care of Animals
HUMAN SUBJECTS APPROVAL
STUDY INFORMATION
Submission ID: HUM00062823
Study Title: Aquaculture and Child Undernutrition in Rural Nepal
Full Study Title (if applicable):
PI: Zachary Stepan
Approval Period: 5/16/2012 -5/15/2013
Expiration Date: 5/15/2013
CONTINUING REVIEW/TERMINATION
Federal regulations require that the Institutional Review Board (IRB) conduct a continuing
review of human subject research at least once per year.
You are receiving this notice because the expiration date (5/15/2013) for your project is
approaching.
At the time this notice was generated, a Continuing Review had not been submitted. Instructions
for doing this can be found online here: Creating a Continuing Review.
The Continuing Review is required to request renewal or terminate the study. The Continuing
Review is not optional. If the study is scheduled to continue beyond the expiration date, submit
the Continuing Review application at least two months prior to the expiration date to allow the
IRB sufficient time to review and approve the project.
AMENDMENTS
All changes to the study, including revised informed consent, must be made via an Amendment,
not a Continuing Review. Instructions for creating and submitting an Amendment can be found
online here: Creating an Amendment.
IMPACT ON RESEARCH
If a Continuing Review is not submitted and approved prior to the expiration date, all project
activity must stop. This includes subject recruitment, experimental procedures or treatments, and
data collection or data analysis, except as necessary to eliminate apparent immediate hazards to
research subjects.
If a lapse in IRB approval occurs, expenditure of federal research funds must stop, including
salary support associated with human subject activities. For more information about this
restriction please contact your Division of Research Development and Administration (DRDA)
representative.
AquaFish/USAID/OSU
CERTIFICATIONS AND ASSURANCES
1. ASSURANCE OF COMPLIANCE WITH LAWS AND REGULATIONS
GOVERNING NON-DISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS
(a)
The recipient hereby assures that no person in the United States shall, on the
bases set forth below, be excluded from participation in, be denied the benefits of, or be
otherwise subjected to discrimination under, any program or activity receiving financial
assistance from USAID, and that with respect to the Cooperative Agreement for which
application is being made, it will comply with the requirements of:
(1)
Title VI of the Civil Rights Act of 1964 (Pub. L. 88-352, 42 U.S.C. 2000d), which prohibits discrimination on the basis of race, color or national origin, in
programs and activities receiving Federal financial assistance;
(2)
Section 504 of the Rehabilitation Act of 1973 (29 U.S.C. 794), which
prohibits discrimination on the basis of handicap in programs and activities
receiving Federal financial assistance;
(3)
The Age Discrimination Act of 1975, as amended (Pub. L. 95-478),
which prohibits discrimination based on age in the delivery of services and
benefits supported with Federal funds;
(4)
Title IX of the Education Amendments of 1972 (20 U.S.C. 1681, et seq.),
which prohibits discrimination on the basis of sex in education programs and
activities receiving Federal financial assistance (whether or not the programs or
activities are offered or sponsored by an educational institution); and
(5)
USAID regulations implementing the above nondiscrimination laws, set
forth in Chapter II of Title 22 of the Code of Federal Regulations.
(b)
If the recipient is an institution of higher education, the Assurances given herein
extend to admission practices and to all other practices relating to the treatment of
students or clients of the institution, or relating to the opportunity to participate in the
provision of services or other benefits to such individuals, and shall be applicable to the
entire institution unless the recipient establishes to the satisfaction of the USAID
Administrator that the institution's practices in designated parts or programs of the
institution will in no way affect its practices in the program of the institution for which
financial assistance is sought, or the beneficiaries of, or participants in, such programs.
(c)
This assurance is given in consideration of and for the purpose of obtaining any and
all Federal grants, loans, contracts, property, discounts, or other Federal financial assistance
extended after the date hereof to the recipient by the Agency, including installment payments
after such date on account of applications for Federal financial assistance which was
approved before such date. The recipient recognizes and agrees that such Federal financial
assistance will be extended in reliance on the representations and agreements made in this
Assurance, and that the United States shall have the right to seek judicial enforcement of this
AquaFish/USAID/OSU
Assurance. This Assurance is binding on the recipient, its successors, transferees, and
assignees, and the person or persons whose signatures appear below are authorized to
sign this Assurance on behalf of the recipient.
2. CERTIFICATION REGARDING LOBBYING
The undersigned certifies, to the best of his or her knowledge and belief, that:
(1)
No Federal appropriated funds have been paid or will be paid, by or on behalf of
the undersigned, to any person for influencing or attempting to influence an officer or
employee of any agency, a Member of Congress, an officer or employee of Congress, or
an employee of a Member of Congress in connection with the awarding of any Federal
contract, the making of any Federal Cooperative Agreement, the making of any Federal
loan, the entering into of any cooperative agreement, and the extension, continuation,
renewal, amendment or modification of any Federal contract, grant, loan, or cooperative
agreement.
(2)
If any funds other than Federal appropriated funds have been paid or will be paid
to any person for influencing or attempting to influence an officer or employee of any
agency, a Member of Congress, an officer or employee of Congress, or an employee of a
Member of Congress in connection with this Federal contract, grant, loan, or cooperative
agreement, the undersigned shall complete and submit Standard Form-LLL, "Disclosure
of Lobbying Activities," in accordance with its instructions.
(3)
The undersigned shall require that the language of this certification be included
in the award documents for all subawards at all tiers (including subcontracts, sub-grants,
and contracts under grants, loans, and cooperative agreements) and that all subrecipients
shall certify and disclose accordingly.
This certification is a material representation of fact upon which reliance was placed when this
transaction was made or entered into. Submission of this certification is a prerequisite for
making or entering into this transaction imposed by section 1352, title 31, United States Code.
Any person who fails to file the required certification shall be subject to a civil penalty of not
less than $10,000 and not more than $100,000 for each such failure.
Statement for Loan Guarantees and Loan Insurance
The undersigned states, to the best of his or her knowledge and belief, that: If any funds have
been paid or will be paid to any person for influencing or attempting to influence an officer or
employee of any agency, a Member of Congress, an officer or employee of Congress, or an
employee of a Member of Congress in connection with this commitment providing for the
United States to insure or guarantee a loan, the undersigned shall complete and submit Standard
Form-LLL, "Disclosure Form to Report Lobbying," in accordance with its instructions.
Submission of this statement is a prerequisite for making or entering into this transaction
imposed by section 1352, title 31, U.S. Code. Any person who fails to file the required statement
AquaFish/USAID/OSU
shall be subject to a civil penalty of not less than $10,000 and not more than $100,000 for each
such failure.
3. PROHIBITION ON ASSISTANCE TO DRUG TRAFFICKERS FOR COVERED
COUNTRIES AND INDIVIDUALS (ADS 206)
USAID reserves the right to terminate this Agreement, to demand a refund or take other
appropriate measures if the Grantee is found to have been convicted of a narcotics offense or to
have been engaged in drug trafficking as defined in 22 CFR Part 140. The undersigned shall
review USAID ADS 206 to determine if any certifications are required for Key Individuals or
Covered Participants.
If there are COVERED PARTICIPANTS: USAID reserves the right to terminate assistance to
or take other appropriate measures with respect to, any participant approved by USAID who is
found to have been convicted of a narcotics offense or to have been engaged in drug trafficking
as defined in 22 CFR Part 140.
4. CERTIFICATION REGARDING TERRORIST FINANCING IMPLEMENTING
EXECUTIVE ORDER 13224
By signing and submitting this application, the prospective recipient provides the certification
set out below:
1.
The Recipient, to the best of its current knowledge, did not provide, within the
previous ten years, and will take all reasonable steps to ensure that it does not and will
not knowingly provide, material support or resources to any individual or entity that
commits, attempts to commit, advocates, facilitates, or participates in terrorist acts, or
has committed, attempted to commit, facilitated, or participated in terrorist acts, as that
term is defined in paragraph 3.
2.
The following steps may enable the Recipient to comply with its obligations
under paragraph 1:
a.
Before providing any material support or resources to an individual or
entity, the Recipient will verify that the individual or entity does not (i) appear on
the master list of Specially Designated Nationals and Blocked Persons, which list
is maintained by the U.S. Treasury’s Office of Foreign Assets Control (OFAC)
and is available online at OFAC’s website :
http://www.treas.gov/offices/eotffc/ofac/sdn/t11sdn.pdf, or (ii) is not included in
any supplementary information concerning prohibited individuals or entities that
may be provided by USAID to the Recipient.
b.
Before providing any material support or resources to an individual or
entity, the Recipient also will verify that the individual or entity has not been
designated by the United Nations Security (UNSC) sanctions committee
established under UNSC Resolution 1267 (1999) (the “1267 Committee”)
AquaFish/USAID/OSU
[individuals and entities linked to the Taliban, Osama bin Laden, or the Al Qaida
Organization]. To determine whether there has been a published designation of
an individual or entity by the 1267 Committee, the Recipient should refer to the
consolidated list available online at the Committee’s website:
http://www.un.org/Docs/sc/committees/1267/1267ListEng.htm.
c.
Before providing any material support or resources to an individual or
entity, the Recipient will consider all information about that individual or entity
of which it is aware and all public information that is reasonably available to it or
of which it should be aware.
d.
The Recipient also will implement reasonable monitoring and oversight
procedures to safeguard against assistance being diverted to support terrorist
activity.
3.
For purposes of this Certificationa.
“Material support and resources” means currency or monetary instruments or
financial securities, financial services, lodging, training, expert advice or assistance,
safehouses, false documentation or identification, communications equipment,
facilities, weapons, lethal substances, explosives, personnel, transportation, and other
physical assets, except medicine or religious materials.”
b.
“Terrorist act” means(i)
an act prohibited pursuant to one of the 12 United Nations
Conventions and Protocols related to terrorism (see UN terrorism
conventions Internet site: http://untreaty.un.org/English/Terrorism.asp); or
(ii)
an act of premeditated, politically motivated violence perpetrated
against noncombatant targets by subnational groups or clandestine agents;
or
(iii)
any other act intended to cause death or serious bodily injury to a
civilian, or to any other person not taking an active part in hostilities in a
situation of armed conflict, when the purpose of such act, by its nature or
context, is to intimidate a population, or to compel a government or an
international organization to do or to abstain from doing any act.
c.
“Entity” means a partnership, association, corporation, or other
organization, group or subgroup.
d.
References in this Certification to the provision of material support and
resources shall not be deemed to include the furnishing of USAID funds or
USAID-financed commodities to the ultimate beneficiaries of USAID assistance,
such as recipients of food, medical care, micro-enterprise loans, shelter, etc.,
unless the Recipient has reason to believe that one or more of these beneficiaries
AquaFish/USAID/OSU
commits, attempts to commit, advocates, facilitates, or participates in terrorist
acts, or has committed, attempted to commit, facilitated or participated in
terrorist acts.
e.
The Recipient's obligations under paragraph 1 are not applicable to the
procurement of goods and/or services by the Recipient that are acquired in the
ordinary course of business through contract or purchase, e.g., utilities, rents,
office supplies, gasoline, etc., unless the Recipient has reason to believe that a
vendor or supplier of such goods and services commits, attempts to commit,
advocates, facilitates, or participates in terrorist acts, or has committed, attempted
to commit, facilitated or participated in terrorist acts.
This Certification is an express term and condition of any agreement issued as a
result of this application, and any violation of it shall be grounds for unilateral
termination of the agreement by USAID prior to the end of its term.
5. CERTIFICATION OF RECIPIENT
By signing below the recipient provides certifications and assurances for (1) the Assurance of
Compliance with Laws and Regulations Governing Non-Discrimination in Federally Assisted
Programs, (2) the Certification Regarding Lobbying, (3) the Prohibition on Assistance to Drug
Traffickers for Covered Countries and Individuals (ADS 206) and ( 4) the Certification
Regarding Terrorist Financing implementing Executive Order 13224 above.
Name of Applicant
Ryan Lankton
Project Representative
Typed Name and Title
Signature
Date
sj t 9. CVS FOR US AND HC PRINCIPAL INVESTIGATORS
JAMES S. DIANA
School of Natural Resources and Environment
University of Michigan
Ann Arbor, MI 48109-1115
Email: jimd@umich.edu
Phone: 734-763-5834
Fax: 734-936-2195
Education
University of Alberta
California State University, Long Beach
California State University, Long Beach
Ph.D., Zoology, 1979
M.A., Biology, 1975
B.S., Marine Biology, 1974
Professional Experience
2009-present
1999-2007
1996-present
1984-1996
1979-1984
Director, Michigan Sea Grant
Associate Dean, School of Natural Resources and Environment
Professor of Fisheries and Aquaculture
Associate Professor and Associate Research Scientist
Assistant Professor and Assistant Research Scientist
Key Publications
Diana, J.S., H.S. Egna, T. Chopin, M.S. Peterson, L. Cao, R. Pomeroy, M. Verdegem, W.T.
Slack, M.G. Bondad-Reantaso, and F. Cabello. 2013. Responsible aquaculture in 2050:
Valuing local conditions and human innovations will be key to success. BioScience
63:255-262.
Cao, L., J.S. Diana, and G.A. Keoleian. 2013. Role of life cycle assessment in sustainable
aquaculture. Reviews in Aquaculture 4:1-11.
Diana, J.S. 2012. Is lower intensity aquaculture a valuable means of producing food? An
evaluation of its effects on near-shore and inland waters. Reviews in Aquaculture 4:234245.
Diana, J.S. 2012. Some principles of pond fertilization for Nile tilapia using organic and
inorganic inputs. Pages 163-177 in C.C. Mischke, editor. Pond Fertilization: Impacts of
Nutrient Input on Aquaculture Production. John Wiley and Sons, Inc.
Cao L., J.S. Diana, G.A. Keoleian, and Q. Lai. 2011. Life cycle assessment of Chinese shrimp
farming systems targeted for export and domestic sales. Environmental Science &
Technology 45:6531-6538.
Yuan, D., Y. Yi, A. Yakupitiyage, K. Fitzimmons, and J.S. Diana. 2010. Effects of addition of
red tilapia (Oreochromis spp.) at different densities and sizes on production, water
quality and nutrient recovery of intensive culture of white shrimp (Litopenaeus
vannamei) in cement tanks. Aquaculture 298:226–238.
Diana, J.S. 2009. Aquaculture production and biodiversity conservation. BioScience 59: 27-38.
Schwantes, V.S., J.S. Diana, and Yang Yi. 2009. Social, economic, and production
characteristics of giant river prawn Macrobrachium rosenbergii culture in Thailand.
Aquaculture 287:120-127
Tain, F. H., and J.S. Diana. 2007. Impacts of extension practice: Lessons from small farm-based
aquaculture of Nile tilapia in Northeastern Thailand. Society and Natural Resources
20:583-595.
Diana, J.S. 2004. Biology and ecology of fishes, Second Edition. Biological Sciences Press.
Carmel, Indiana.
Yang Yi, C.K. Lin, and J.S. Diana. 2002. Techniques to mitigate clay turbidity problems in
fertilized earthen fish ponds. Aquacultural Engineering 27:39-51.
Diana, J.S., and C.K. Lin. 1998. The effects of fertilization on growth and production of Nile
tilapia in rain-fed ponds. Journal of the World Aquaculture Society 29:405-413.
Diana, J.S. 1997. Feeding strategies. Pages 245-263 in H. Egna and C. Boyd, editors. Dynamics
of pond aquaculture. CRC Press, Boca Raton, Florida.
Diana, J.S., J.P. Szyper, T.R. Batterson, C.E. Boyd, and P.H. Piedrahita. 1997. Water quality in
ponds. Pages 53-71 in H. Egna and C. Boyd, editors. Dynamics of pond aquaculture.
CRC Press, Boca Raton, Florida.
Diana, J.S., C.K. Lin, and Y. Yi. 1996. Timing of supplemental feeding for tilapia production.
Journal of the World Aquaculture Society 27:410-419.
Diana, J.S., and C.K. Lin. 1995. Supplemental feeding of tilapia in fertilized ponds. Journal of
the World Aquaculture Society 25:497-506.
Lin, C.K., and J.S. Diana. 1995. Co-culture of catfish (Clarias macrocephalus x C. Gariepinus)
and tilapia (Oreochromis niloticus) in ponds. Aquatic Living Resources 8:449-454.
Diana, J.S., D. Dettweiler, and C.K. Lin. 1990. Effect of Nile tilapia Oreochromis niloticus) on
the ecosystem of aquaculture ponds, and its significance to the trophic cascade
hypothesis. Canadian Journal of Fisheries and Aquatic Sciences 48:183-190.
Key Grants Received
Oregon State University. 1983-present. Improving sustainability and reducing environmental
impacts of aquaculture systems in China and South and Southeast Asia.
National Science Foundation. 2009-present. Collaborative research: improving the
environmental sustainability of shrimp aquaculture through microbial resource management.
Mousetrap Foundation. 2009-present. Investigating ecosystem-level impacts of shrimp
farm eutrophication in Thailand through stable isotope ratio analysis, and an evaluation of
eco-certification's potential to support related best management practices.
Graham Environmental Sustainability Institute. 2006-present. Development of sustainable
shrimp aquaculture.
Madhav Kumar Shrestha
Department of Aquaculture
Agriculture and Forestry University
Rampur, Chitwan, NEPAL
Email: madhavshrestha1954@gmail.com
Phone: 977–56-592272 (Office), 977-56-591264 (Residence)
Fax: 977-56-591021
Academic Qualifications:
Post Doc. Asian Institute of Technology (AIT), Thailand (1996-97)
D. Tech. Sc. (Ph.D.) Aquaculture, Asian Institute of Technology (AIT), Bangkok, Thailand,
1994.
M. Sc. Aquaculture, Asian Institute of Technology (AIT), Bangkok, Thailand, 1989.
M. Sc. Zoology (Fisheries), Tribhuvan University (TU), Kathmandu, Nepal, 1979.
Employment History:
04/011-date. Professor of Aquaculture, IAAS, TU, Nepal.
08/'99-04/011. Reader of Aquaculture, IAAS, TU, Nepal.
06/'87-07/'99. Lecturer of Aquaculture, IAAS, TU, Nepal.
04/'82-06/'87. Assistant Fisheries Development Officer, Department of Agriculture, Nepal.
12/'79-04/'82. Assistant Lecturer of Zoology, IAAS, TU, Nepal.
Other Professional responsibilities:
Post Graduate Studies Coordinator, Agriculture and Forestry University (12/2012 - present)
Editor-in Chief, Journal of Agriculture and Animal Science, (1999-2005)
Director, Directorate of Research and Publications, IAAS, Rampur, Chitwan, Nepal (20'02–
2005)
Coordinator, Postgraduate program, IAAS, Rampur, Chitwan, Nepal (06/2001–12/2001)
Department Chair, Aquaculture Department, IAAS (1995-96; 2005-2008)
Subject Committee Chair, Aquaculture Subject Committee (2007-2010; 2010-date)
Publications:
Books, Manuals, Proceedings (7)
International Journal articles (14)
National Journal articles (15)
Proceeding papers (15);
Professional articles (8)
Research Reports (26)
Abstracts (51)
Papers presented in Conference/Seminar/workshops: 60
Conference/Seminar/Workshop attended: 50
Research Handled:
Coordinator: Woman in Aquaculture Project in Mid Hills Nepal, Aquaculture without Frontier
(AwF project), Phase I & Phase II (2009 – date)
Host Country Co-PI: Aquaculture and Fisheries Collaborative Research Support Program
(AquaFish CRSP). IAAS-SOU Collaborative Project, USAID Funded, Phase 1, II,
III & IV (2001- date)
Project Coordinator: NARDF Project # 218 (2006-2009); 513 (2008-20011) & 605 (2009-2011)
Country Coordinator: Enhancing the Impacts of Decentralised (fish) Seed Production in
Bangladesh, India and Nepal. Asian Innovation Challenge Fund, DFID (2009-2011)
Host Country Coordinator: Asia Link Project I & II, EU Funded (2005-2010)
Coordinator: Women in Aquaculture in Nepal, WDP-German Committee Funded (2000-2004)
Technical Advisor: Community Fish Production and Marketing Project (CFPMP), Canadian
Cooperation Office Funded (2004-2007)
Seminar/Workshop organized:
AQUAFISH CRSP WORKSHOP, IAAS, RAMPUR (2011)
SYMPOSIUM ON 'SMALL-SCALE AQUACULTURE FOR INCREASING RESILIENCE OF RURAL
LIVELIHOODS IN NEPAL' HOTEL RADISSON, KATHMANDU (2009)
AQUAFISH CRSP WORKSHOP, IAAS, RAMPUR (2009)
AQUACULTURE CRSP WORKSHOP, CENTRAL FISHERIES BUILDING, BALAJU, KATHMANDU
(2007)
ALGAL BIOASSAY WORKSHOP, IAAS, RAMPUR (2005)
Professional Membership:
Nepal Fisheries Society,
Nepal Animal Science Society,
Natural History Society of Nepal,
Asian Fisheries Society,
World Aquaculture Society,
WAS Pacific Chapter
10. AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Title of Proposal:
Development of more efficient and environmentally sustainable aquaculture systems for Nepal.
For Lead Project PI:
James Diana
x
All co-authors on publications within the past four years, including pending publications and
submissions
H.S. Egna, T. Chopin, M.S. Peterson, L. Cao, R. Pomeroy, M. Verdegem, W.T. Slack, M.G. BondadReantaso, F. Cabello, Mychek-Londer, J.G., D.B. Bunnell, W. Stott, J.R. P.French III, M.A. Chriscinske.
C.C. Mischke, L. Cao, G.A. Keoleian, Q. Lai. J.C. Boase, M.V. Thomas, J.A. Chiotti L. Lui, L. Kang, Y.
Yaling, Y. Jun, Y. Yi, L. Liu, K. Li, T. Chen, X. Dai, M. Jiang, M.K. Shrestha, R.L. Sharma, K. Gharti,
Jiang Min, Yu Gen-ding, Dai Xilin, Liu Liping, Gu Deping, Hu Weiguo, S.A.S. Ahmad, A.N. Bart, J.E.
Rakocy, D. Yuan, A. Yakupitiyage, K. Fitzimmons, V.S. Schwantes,
x
All collaborators on research projects within the past four years, including current and
planned collaborations
R. Kinnunen, J. Read, L. Raskin, A. Briones, D. Bunnell, C. Madenjian, J. Londer, D. Rowe, R.
Bierbaum
x
All thesis or postdoctoral advisees/advisors within the past four years
S. David, L. Cao, K. Battige, J. Londer, T. Brookhart, S. Ledwin, J. Nohner, K. Birkett, K. HayseGregson, S. Kempke
x
All persons in your field with whom you have had a consulting/financial arrangement/other
conflict-of-interest within the past four years
None
Please complete a separate list for each HC PI and subcontracting PIs (not necessary for co-PIs)
HC PI:
Madhav Shrestha
The following information is needed to establish instances where a conflict-of-interest may exist between
PIs and prospective technical peer-reviewers and external program evaluators. For each PI (Project Lead
and other US & HC PI) please provide a list with the names of the following people and associated
institutions or organizations.
x
All co-authors on publications within the past four years, including pending publications and
submissions
N. Pandit, M. Nakamura, R.B. Mandal, D.K. Jha, J. Pant, S. Rai, M.B. Karki, J.S. Diana
x
All collaborators on research projects within the past four years, including current and
planned collaborations
J.S. Diana
x
All thesis or postdoctoral advisees/advisors within the past four years
N. Pandit, S. Rai
x
None
All persons in your field with whom you have had a consulting/financial arrangement/other
conflict-of-interest within the past four years
11. LIST OF FIVE REVIEWERS
Dr. Ted Batterson
Department of Fisheries and Wildlife
Michigan State University
East Lansing, MI 48824-1222
Email: batters2@msu.edu
Expertise – aquaculture research and development
Dr. Kevin Hopkins
Pacific Aquaculture and Coastal Resources Center
University of Hawaii
200 W. Kawili St.
Hilo, HI 96720-4091
Email: Hopkins@hawaii.edu
Expertise – international aquaculture and development
Dr. Charles Mischke
Delta Research and Extension Center
Mississippi State University
Stoneville, MS 38776
Email: cmischke@drec.msstate.edu
Expertise: pond fish production
Dr. David Little
Institute of Aquaculture
University of Stirling
Stirling, Stirlingshire, FK9 4LA, UK
Email: dcl1@stir.ac.uk
Expertise: rural aquaculture in poor countries
Dr. Peter Edwards
Asian Institute of Technology
Km 41 Paholyothin Highway
Bangkok, Thailand 10501 Thailand
E-mail: pedwards@inet.co.th
Expertise: rural aquaculture in poor countries