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20140821a.pdf
IR-4 Ornamental Horticulture Program
Research Report Cover Sheet
Trial: 2014 WA
Researcher(s) Gary Chastagner
Washington State University
Affiliation:
Pullayup
WA
PhoneNumber: (253) 445-4528
chastag@wsu.edu
Email:
ProjectTitle:
Protocol #:
PR#
31946
32042
31947
32041
31948
31949
31950
31951
31952
Botrytis Efficacy
14-001
Research Target
Crop/Plant
Product
EPA Reg. # Production Site
Botrytis tulipae
Tulip
BAS703 01F
Botrytis tulipae
Tulipa sp.
Fluxapyrosad + pyraclostrobi BASF
-
Botrytis tulipae
Tulip
Chipco 26019 N/G
432-889
Botrytis tulipae
Tulipa sp.
Iprodione
Bayer
Status
Field In-Ground
C
Field In-Ground
C
Field In-Ground
C
Botrytis tulipae
Tulip
F9110
-
Botrytis tulipae
Tulipa sp.
F9110
FMC
Botrytis tulipae
Botrytis tulipae
Tulip
Tulipa sp.
Pageant 38WG
Boscalid + Pyraclostrobin
7969-251
BASF
Field In-Ground
C
Field In-Ground
C
Botrytis tulipae
Tulip
Proud 3
-
Botrytis tulipae
Tulipa sp.
Thyme oil (5.6%)
Biohumanetics
Botrytis tulipae
Botrytis tulipae
Tulip
Tulipa sp.
S2200 4SC
Mandestrobin
Valent
Field In-Ground
C
Field In-Ground
C
Field In-Ground
C
Field In-Ground
C
Botrytis tulipae
Tulip
SP2770 10WP
-
Botrytis tulipae
Tulipa sp.
SP2770
SePro
Botrytis tulipae
Tulip
SP2773
-
Botrytis tulipae
Tulipa sp.
SP2773
SePro
Botrytis tulipae
Botrytis tulipae
Tulip
Tulipa sp.
Torque 3.6SC
Tebuconazole
1001-87
Cleary
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Narrative Summary (Results/Discussion)
A total of 9 products were evaluated in two trials for their effectiveness in controlling Botrytis tulipae on field-grown
“Dynasty” tulips. Trial 1 included 7 products and trial 2, which will be referred to as the SePRO trial included 2 SePRO
products. Non-sprayed plants served as checks in both trials. Disease pressure progressed steadily in all test cells during
these trials. By the end of the 120-day test, the average severity rating of the non-sprayed checks was 8.0. The ratings of
all the treatments in trial 1 ranged from 1.3 to 8.8. The average severity rating for the SePRO treatments in trial 2 ranged
from 5.3 to 9.3. No phytotoxicity was observed in these trials.
The ANOVA analysis of data from trial 1 indicated that treatments had a highly significant (P<0.001) effect on disease
severity, the percent of blighted flowers, the height of flowers, and the weight of harvested bulbs. All treatments except
Proud 3 had severity ratings and percent blighted flowers that were significantly less than the checks (Table 1). Pageant
38WG and BAS 703 01F were the most effective materials in reducing disease development. Two biofungicides were
included in this trial. Although Proud 3 was ineffective, applications of F9110 did significantly reduce disease
development of the foliage and flowers. BAS 703 01F and Pageant 38WG treated plants also had significantly more
flowers that were greater than 13” tall (Table 2) and yielded more bulbs greater than 12cm than the non-treated checks
(Table 3).
The ANOVA analysis of data from trial 2 indicated that treatments had a highly significant (P<0.001) effect on disease
severity, the percent of blighted flowers, the height of flowers, and the weight of harvested bulbs. Both rates of SP2773
had significantly lower severity ratings than the non-treated check (Table 4). The high rate of SP2773 had significantly
less blighted flowers and more flowers greater than 13” tall than the non-treated check (Table 4 & 5). The high rate of
SP2773 also yielded significantly more bulbs that were 10-12 cm than the non-treated check (Table 6). Plants sprayed
with SP2770 had very high disease ratings and produced significantly fewer 10-12 cm bulbs than the non-treated check
(Table 6).
The data from these trials indicate that several of the fungicides tested have the potential to provide effective control of
Botrytis on tulips. This includes the biofungicide F9110, which showed promise in reducing disease development on the
foliage and flowers even under relatively high disease pressure.
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Field Layout
Results Table
Table 1. Effect of foliar fungicides applications on the percent of blighted flowers on April 30, 2014 (Day 62) and
the severity of Botrytis on “Dynasty” tulip foliage on May 12, 2014 (Day 74).
% Blighted
PRnumbers
Prod/100 gal/A
Flowers
4
31948
100.0a
Proud 3
4 qts
1
100.0a
Check
3
31947
30.2cd
F9110
24 fl oz
12
32042
59.4b
Chipco 26019 N/G
16 oz
5
31949
72.0b
S2200
7.5 fl oz
9
31952
56.6bc
Torque 3.6SC
8 fl oz
11
32041
20.1d
Pageant 38WG
14 oz
2
31946
19.2d
BAS 703 01F
8 oz
1
Numbers in columns followed by the same letter are not significantly different, P<0.05, Tukey’s
Range (HSD) Test. V-10135 was initially included in the protocol but was withdrawn from the trial.
TRT
TRT/PRODUCT
Severity
8.8a
8.0a
3.3b
3.3b
2.8bc
2.8bc
1.5c
1.3c
Studentized
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Table 2. Effect of foliar fungicides on flower height on April 10, 20141 (Day 42).
Flower Heights
< 10”
TRT
TRT/PRODUCT
PRnumbers Prod/100 gal/A
10-13”
>13”
Total
2
31946
2.0b
11.8a
21.0a
34.8ab
BAS 703 01F
8 oz
11
32041
6.3ab
17.8ab
33.3ab
Pageant 38WG
14 oz
9.3a
5
S2200
31949
7.5 fl oz
5.3ab
15.0a
15.0abc
35.3ab
9
31952
8.8ab
13.8a
14.0abc
36.5a
Torque 3.6SC
8 fl oz
12
32042
5.5ab
15.8a
13.3abc
34.5ab
Chipco 26019 N/G
16 oz
3
F9110
31947
24 fl oz
10.8ab
15.5a
6.0abc
32.3ab
4
31948
11.5ab
16.5a
2.0bc
30.0ab
Proud 3
4 qts
1
16.0a
11.5a
1.0c
28.5b
Check
1
Numbers in columns followed by the same letter are not significantly different, P<0.05, Tukey’s Studentized
Range (HSD) Test.
Table 3. Effect of fungicide applications on the weight of harvested bulbs on June 27, 20141 (Day 120).
Wt (g) of Bulbs
TRT
TRT/PRODUCT
PRnumbers Prod/100 gal/A < 8 cm 8-10 cm 10-12 cm >12 cm
Total
2
31946
225.6a
95.2ab 314.1ab
416.0a
1050.9a
BAS 703 01F
8 oz
11
32041
206.6a
91.7b 166.7ab
314.6ab
779.6ab
Pageant 38WG
14 oz
3
F9110
31947
24 fl oz
241.6a 110.9ab 271.1ab
163.6abc
787.2ab
5
31949
238.8a 126.4ab 343.1a
156.4abc
864.6ab
S2200
7.5 fl oz
9
31952
223.9a 204.5a 238.6ab
151.3bc
818.3ab
Torque 3.6SC
8 fl oz
12
Chipco 26019 N/G
32042
16 oz
216.2a 114.3ab 289.8ab
136.3bc
756.6ab
4
31948
183.8a 134.7ab 110.2b
0.0c
428.7b
Proud 3
4 qts
1
200.7a 125.1ab 124.5ab
0.0c
450.3b
Check
1
Numbers in columns followed by the same letter are not significantly different, P<0.05, Tukey’s Studentized Range
(HSD) Test.
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Table 4. Effect of SePRO fungicide applications on the number of blighted flowers on April 30, 2014 (day 62) and
the severity of Botrytis on “Dynasty” tulip foliage on May 12, 20141 (Day 74).
% Blighted
TRT
TRT/PRODUCT
PRnumbers
Prod/100 gal/A
Flowers
Severity
6
31950
100.0a
9.8a
SP2770
2.66 lb
1
Check
100.0a
8.0a
7
31951
99.1ab
6.0bc
SP2773
1.66 lb
8
31951
93.4b
5.3c
SP2773
3.313 lb
1
Numbers in columns followed by the same letter are not significantly different, P<0.05, Tukey’s Studentized Range
(HSD) Test.
Table 5. Effect of SePRO foliar fungicides on flower Height on April 10, 20141 (Day 42).
Flower Heights
< 10”
TRT
TRT/PRODUCT
PRnumbers
Prod/100 gal/A
10-13”
> 13”
Total
8
31951
6.8b
17.0ab
9.0a
32.8a
SP2773
3.313 lb
7
31951
12.0b
18.0a
1.8ab
31.8a
SP2773
1.66 lb
1
16.0ab
11.5ab
1.0ab
28.5a
Check
6
31950
24.0a
7.0b
0.0b
31.0a
SP2770
2.66 lb
1
Numbers in columns followed by the same letter are not significantly different, P<0.05, Tukey’s Studentized Range
(HSD) Test.
Table 6. Effect of SePRO fungicide applications on the weight of harvested bulbs on June 27, 20141 (Day 120).
Wt (g) of Bulbs
TRT TRT/PRODUCT
PRnumbers
Prod/100 gal/A < 8 cm 8-10 cm 10-12 cm >12 cm
Total
8
31951
219.8a
198.3a 220.6a
20.2a
658.9a
SP2773
3.313 lb
7
31951
220.5a
156.2a 175.1ab
7.3a
559.0ab
SP2773
1.66 lb
1
200.7a
125.1a 124.5b
0.0a
450.3bc
Check
6
31950
229.2a
124.0a
22.7c
0.0a
375.9c
SP2770
2.66 lb
1
Numbers in columns followed by the same letter are not significantly different, P<0.05, Tukey’s Studentized Range
(HSD) Test.
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Experiment Information
Experimental Design:
Number of Reps:
Randomized Complete Block
4
Materials & Methods/Recordkeeping
Please fill out the information below or attach a separate document with comparable information.
Bulbs used in this trial were planted on October 4, 2013 in an area that has been planted with tulip bulbs for the past three
seasons. Field design consisted of 5 rows spaced on 40” centers. Each row contained 10 3-foot-long planting cells with 3
feet between the planted cells. Prior to planting, all cells were fertilized by incorporating Plantacoat 14-14-14 fertilizer @
438 lbs. per acre in an 8 to 12-inch wide band just below the soil surface within the rows where the bulbs were to be
planted. After placing 35 bulbs in each planting cell, bulbs were treated with an in-furrow 18” wide band of Terraclor
75WP at 3 lbs/1000 ft of row prior to hilling to control soil-borne diseases. On December 9, 2013, the plot was inoculated
with previous season tulip foliage that was dried, finely chopped, and evenly spread over the hills. Initial emergence of
plants was observed the first week of February 2014. On February 19, 2014, cells were assigned to blocks based on the
incidence of disease already present. Disease incidence was rated on a scale of 0 to 10 where 0 = none, 1 = 1-10%, 2 =
11-20%,….., and 10 = 91-100% of plant foliage were diseased. Initial incidence ratings ranged from 0-2. All Treatments
were applied on 1 and 2-week intervals starting on February 27, 2014. SePRO products were initially applied on February
27th, but there was a 3-week interval before the second applications were applied.
Disease was visually monitored and severity was rated on a scale of 0 to 10 where 0 = none, 1 = 1-10%, 2 = 11-20%, …..,
and 10 = 91-100% of the foliage was killed. Disease severity ratings were taken on days 32, 46, 62, and 74. Flower
heights were collected on April 10, 2014 (Day 42) and the number of blighted flowers was assessed on April 30, 2014
(Day 62). Blighted flowers were based on the number of flowers per cell where the disease had spread into the stem.
Flower height was a measurement of the length of the flower stem from soil line to base of flower. Tulip bulbs were
harvested on June 25, 2014 and sorted and weighted on June 27, 2014 (Day 120).
Lesions on foliage
Blighted flowers
Lesion growing down flower
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Name(s) of Personnel Conducting Research: Gary Chastagner and Annie DeBauw
Location of Trial (city/state): WSU Puyallup Research and Extension Center
Use Site (greenhouse/shadehouse/field container/etc): Field
Sorted Bulbs on June 27, 2014
Crop History
Crop Cultivar/Variety:
Date of Seeding:
Date of Emergence:
Bed size:
Row spacing
Seeding density:
Harvest Date:
“Dynasty” Tulips
October 4, 2013
February 3, 2014
960 ft2
40” centers
35 bulbs/3 ft cells
June 25, 2014
Product(s) applied prior to start of experiment:
Product
Rate
Application
Type
Plantacoat 14-14-14 438 lbs/acre
In-furrow
Terraclor 75WP
3 lbs/1000 ft of
In-furrow
row
Surflan + Buccaneer 0.5 fl oz + 0.6 fl oz Broadcast
Ranger Pro
2.33 oz/gal
Spot Spray
Date of
Application
10/3/2013
10/4/2013
Crop Growth
Stage
Prior to planting
Prior to hilling
10/30/2013
After hilling
2/26/14
Plants 2” w/no
Application Volume
15.2 g per foot of row
408g/3 gal/300 feet
of row
5 fl oz + 6 fl oz/10 L
/0.23 A
14 oz/6 gal
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
buds present
Product(s) applied during experiment (including treatments, fertilizers, etc):
PRNUMBERS
PRODUCT/CODE
COMMON NAME
31946
BAS 703 01F
proprietary
31947
F9110
extract of Lupinus
31948
Proud 3
thyme oil (5.6)
31949
S2200 4SC
mandestrobin
31950
SP2770 10 WP
proprietary
31951
SP2773
proprietary
Torque 3.6SC
31952
tebuconazole
32041
Pageant 38 WG
pyraclostrobin (12.8). boscalid (25.2)
32042
Chipco 26019 N/G
iprodione
MANUFACTURER
BASF
FMC
BioHumanetrics
BuFarm
SePRO
SePRO
NuFarm
BASF
OHP
Treatment List and Application Dates
Rate/100
Application
Application
Trt #** TRT/PRODUCT
PRnumbers
Batch #
gal/A
Interval
Dates1
1
Check
31946
270037
14 day
2
BAS 703 01F
8 fl oz
C
31947
D31-230713
7 day
A
3
F9110
24 fl oz
31948
HG-810-09252012
7 day
A
4
Proud 3
4 qts
31949
VTC-1324-39
14 day
C
5
S2200 4SC
7.5 fl oz
31950
None provided
7day
B*
6
SP2770 10WP
2.66 lb
31951
None provided
14 day
D*
7
SP2773
1.66 lb
31951
None provided
14 day
D*
8
SP2773
3.313 lb
31952
13203VL001
14 day
C
9
Torque 3.6SC
8 fl oz
32041
2236S02EJ
14 day
C
11
Pageant 38WG
14 oz
32042
None provided
14 day
C
12
Chipco 26019 N/G
16 oz
1
Dates: 1 = 2/27/14, 2= 3/7/14, 3 3/13/14, 4 = 3/21/14, 5 = 3/31/14, 6 = 4/8/14, 7 = 4/14/14, 8 = 4/22/14
A = 1, 2, 3, 4, 5, 6, 7, 8; B = 1, 4, 5, 6, 7, 8; C = 1, 3, 5, 7; D = 1, 4, 5, 7.
*There was a 3-week interval between the first and second applications of these products.
**Treatment 10 was initially included in the protocol but was withdrawn from the trial.
Ornamental Horticulture Program
Research Report Form
Researcher:
Project Title:
Protocol #:
Gary Chastagner and Annie DeBauw
Date:
8/7/2014
T-114; IR-4 Efficacy of Foliar Fungicides to Control Botrytis on “Dynasty” Tulips
14-001
PRnumbers: 31946, 32042, 31947, 32014, 31948, 31949,
31950, 31951, 31952
Photos
See T-114; IR-4 Botrytis tulipae Photos file for representative treatment effects.
Data Collected
Please describe data collected and scoring system. Also include the dates data were collected.
Disease incidence was rated on a scale of 0 to 10 where 0 = none, 1 = 1-10%, 2 = 11-20%,….., and 10 = 91-100% of plant
foliage were diseased. Disease severity was rated on a scale of 0 to 10 where 0 = none, 1 = 1-10%, 2 = 11-20%, ….., and
10 = 91-100% of the foliage was killed. Disease severity ratings were taken on days 32, 46, 62, and 74. Flower heights
were collected on April 10, 2014 and the number of blighted flowers was assessed on April 30, 2014. Blighted flowers
were based on the number of flowers per cell where the disease had spread into the stem. Flower height was a
measurement of the length of the flower stem from soil line to base of flower. Tulip bulbs were harvested on June 25,
2014 and sorted and weighted on June 27, 2014.
Data analysis: Data were analyzed using ANOVA (GLM SAS). Treatment means were separated using Tukey’s
Studentized Range (HSD) Test.
Application Equipment:
During the growing season, weeds were controlled with a combination of Surlan @ 0.5 fl oz / 1000 sq. ft as well as
broadcast and spot applications of Buccaneer and Ranger Pro using a Solo backpack sprayer. For broadcast applications,
sprayer was equipped with a Spray Systems TK 2 nozzle. For spot applications sprayer was equipped with a 8003 LP
Tee-jet nozzle. For both types of weed control, applications were made at 15 psi in the indicated volumes of water. All
trial treatment applications were applied with a CO2 sprayer equipped with an 8002 Teejet nozzle at 15 psi in the indicated
volume of water.
Raw Data
See T-114; IR-4 Botrytis tulipae Data file for raw data
Environmental conditions during the experiment:
See T-114; IR-4 Botrytis tulipae Data file for outdoor environmental conditions.