DEP Quality Assurance Requirements for Sample Collection &
DEP Quality Assurance Requirements for Sample Collection & Field Testing Department of Environmental Protection Environmental Assessment Section Bureau of Laboratories Why Use the SOPs? QA Rule – Chapter 62-160, F.A.C. DEP SOPs for Field Activities Sample Collection Preservation & Holding Times Mandatory Documentation Quality Control Field Measurements CERP QASR Where to Find: www.dep.state.fl.us/labs/sop/index Department of Environmental Protection Standard Operating Procedures for Field Activities DEP-SOP-001/01 Use for: Training and Daily Use FLORIDA DEPARTMENT OF ENVIRONMENTAL PROTECTION 2600 BLAIR STONE ROAD TALLAHASSEE, FL 32399-2400 Bureau of Laboratories Environmental Assessment Section February 1, 2004 1000 General Sampling FS Field Sampling 2000 General Aqueous General Direct Grab 2000 Aqueous 2100 Surface Water Surface Grabs Intermediate Devices 3000 Soil 2200 Groundwater Water Depth Grab Pumps 4000 Sediment 2300 Drinking Water Automatic Samplers 5000 Waste 2400 Wastewater 6000 Biological Tissue 7000 Biological DEP SOP Structure Naming Conventions 8000 Miscellaneous FA FC FS FM Administrative & Quality Systems Field Cleaning Sampling Procedures Mobilization & Laboratory FQ Quality Control FD Documentation FT Field Testing Key Sampling Considerations Data Quality Objectives Representative Samples Sample Integrity Documentation Administrative Approvals & Quality System Management Intent and purpose of the SOPs Description of SOP format Regulatory requirements for use of the SOPs Approval of alternative field procedures Quality system management guidelines Proficiency requirements for biological sampling Field performance audit checklists Definitions for matrices, analytes, other terms FD 1000 Quality System Must Have a Quality Manual Quality System Design QA Policy and Procedure QA Personnel Functions Available upon Request FA 3000 Auditing You may be audited by External Organizations!! Sampling Performance (Field Audit) Data Usability (Documentation Audit) Audit Criteria DEP SOPs Any approved alternative procedures Project Data Quality Objectives Internal Audits Recommended Guidance checklists in Appendix to FA 1000 or at: http://www.dep.state.fl.us/labs/library/index.htm FA 4000 Documentation Universal record-keeping requirements Required records for each DEP SOP Requirements also apply to: Electronic (digital) data Video, film, maps, other media Documenting transmittal to labs or others Evidentiary (legal) chain-of-custody Example forms FD 1000 Good Documentation Habits for Verification and Defensibility Records must be: Intact & easily understood Linked to all field data and analytical result Note & correct errors without obliteration Signature or initials indicate functions and responsibilities Retain records for 5 years after project completion (Legal Cases Longer??) “When in doubt, write it down” FD 1000 Typical Facts to Record Unique identification of sample containers (analyte group) Number of sample containers for each unique container identification Site and sampling point identification and matrix Sample type (grab, composite, equipment blank, trip blank, etc.) Sampler names, initials or signatures Sample collection date, time, depth, flow rate Sample preservation and preservation checks Notes about sample problems Ambient field conditions Monitor well purging and stabilization log Sampling and purging equipment used Equipment decontamination log Equipment maintenance log Field testing measurements Field meter calibration records Links with site maps, videos, photos Transmittal of samples to lab or other party FD 1000 Decontamination “Performance-Based” Cleaning Cleaning Reagents & Analyte-Free Water General Cleaning Procedures Cleaning Specific Equipment Types Sample Container Cleaning Cleaning Heavily Contaminated Equipment Cleaning Procedure Tables in Appendix FC 1000 Performance-Based Cleaning Cleaning must: Be Effective Use non-contaminating techniques Demonstrate Effectiveness with Blanks and other QC Suggested Procedures in Appendix Cleaning to meet a set of specifications rather than following a rigid, required procedure FC 1000 Mandatory Sampling QC Handle and Document Exactly like the Associated Environmental Samples: Sampling QC Blanks Required for: Volatile Organics Extractable Organics Metals and Ultra-Trace Metals Inorganic Non-Metallics Radionuclides TRPH, FL-PRO and Oil & Grease Volatile Inorganics Aggregate Organics (except BOD, cBOD) Unique Container ID Preservation Documentation Type of QC sample Date and time of collection Preservatives used and preservation checks Type & source of analyte-free water for blanks Sampling equipment used to collect QC sample Procedure used to collect QC sample Transport Storage Link QC sample records with the associated environmental sample documentation FQ 1200 Mandatory Blank Frequencies 5% equipment blanks per matrix and analyte for each project 5% field blanks per matrix and analyte each project (if no equipment used) One equipment blank or field blank per matrix and analyte per year for each project Trip blank in each ice chest (each trip) FQ 1230 Equipment Blanks Collect Pre-Cleaned Equipment Blank before Equipment is used during the Sampling Event Collect Field-Cleaned Equipment Blank after Equipment is Cleaned during the Sampling Event Collect on-site: rinse sampling equipment (including filtration equipment) with analyte-free water FQ 1200 Field Blanks Use when an environ- mental sample is collected with the sample container (no other equipment used for collection of the sample) Collect on-site: pour analyte-free water into clean sample containers FQ 1214 Trip Blanks (VOCs Only) Lab or field team fills VOC vials with analyte-free water before sampling trip Handle same as VOC samples Accompanies empty VOC vials & VOC samples (shipment, transport & storage) One trip blank in each ice chest or shipping (transport) container Prepare trip blanks for each sampling trip Remain sealed (unopened) until analysis FQ 1213 General Sampling Procedures General Cautions and Instructions Cross-Contamination Prevention General Procedure for Composite Samples Sample Container and Equipment Rinsing Placement of Fuel-Powered Equipment Sample Preservation and Preservation Checks Preventive Maintenance of Equipment and Instruments Handling Hazardous and Site-Derived Waste Sample Custody and Tracking Procedures Tables: Sample Preservation, Holding Times & Container Types Construction Materials & Approved Sampling Equipment FS 1000 Table FS 1000-2 Acceptable Materials to be Used for Each Analyte Group Equipment Type and Construction Permissible Analyte Groups for Each Equipment Type Restrictions & Precautions for Using Specific Equipment Configurations Table FS 1000-3 CONSTRUCTION TUBING HOUSING EQUIPMENT Surface Water Intermediate containers such as SS, Teflon, Teflon- N/A pond sampler, scoops, beakers, coated, HDPE, buckets, and dippers PP Glass N/A Non-inert Nansen, Kemmerer, Van Dorn, Alpha and Beta Samplers, Niskin (or equivalent) N/A SS, Teflon, Teflon- N/A coated, HDPE, PP Non-inert N/A USE Grab sampling PERMISSIBLE ANALYTE GROUPS RESTRICTIONS AND PRECAUTIONS All analyte groups None All analyte groups except boron and fluoride All analyte groups except volatile and extractable organics Specific depth grab All analyte groups sampling None None None All analyte groups except volatile and extractable organics All analyte groups None None DO Dunker SS, Teflon, glass, N/A HDPE, PP Water column composite sampling Bailers – double valve SS, Teflon, HDPE, N/A PP Non-inert N/A Grab sampling All analyte groups None Grab sampling All analyte groups except volatile and extractable organics SS, Teflon, Specific depth PE, PP sampling All analyte groups except volatile and extractable organics None If sampling for metals, the tubing must be non-metallic if not SS Silicone tubing in pump head Must be variable speed Peristaltic pump N/A Extractable organics N/A Non-inert All analyte groups except volatile and extractable organics ; configured as specified in Figure FS 1000-1, or use tubing constructed with approved materials in the pump head Silicone tubing in pump head Must be variable speed Tables FS 1000-1 to 1000-3 Equipment Type and Construction Permissible Analyte Groups for Each Equipment Type Restrictions & Precautions for Using Specific Equipment Configurations Tables FS 1000-4 to 9 Preservation, 1000-4: 40 CFR PartHolding Times, Containers 136 Table II 1000-5: Analytes Not Listed in 40 CFR Part 136 1000-6: Sediments, Soils & Residuals 1000-7: Soil VOCs (SW 846, Method 5035) 1000-8: Exceptions for Drinking Water 1000-9: Biosolids Contamination Prevention Rinse all sampling equipment with sample water before collecting samples and discard rinse water away from sampling point Do not rinse sample containers for O&G, TRPH, FL-PRO, microbiology or containers pre-dosed with preservatives Collect from least to most contaminated source Collect upwind and away from fuel-powered equipment Keep equipment, containers and supplies clean until use Don’t put pump tubing, meter probes, other implements or fingers into sample containers Segregate: Highly contaminated samples Incompatible samples Keep ice chests and other transport containers clean between sampling trips Change gloves between sampling points or sources and if torn or contaminated FS 1002 General Aqueous Sampling General Collection, Filtration and Preservation Procedures for Aqueous Samples Instructions for Specific Analyte Groups Metals Extractable Organics (SVOCs) Volatile Organics (VOCs) Bacteria Samples Hydrocarbons (O&G, TRPH, FL-PRO) Radioisotope Samples and Radon Cyanide Samples FS 2000 Preserving Samples Dechlorinate and/or filter (if applicable) before preserving Preserve per tables FS 1000-4 through FS 10009 Preserve immediately (within 15 minutes of collection) Use ice to keep samples @ 4ºC Check pH of pH-preserved samples (see check frequencies in FS 2000) with narrow range pH paper (including pre-preserved containers) Don’t dip pH paper into the sample pH checks not required for VOCs Carry and add extra preservative if required by pH check Avoid excess preservative (can dilute sample or cause analytical problems) Use refrigeration or ice for automatic samplers FS 2000 & FS 2001 Filtering Samples Perform on-site Pore size & filter type: 0.45 ȝm Filtration may be a preservation requirement Filter groundwater samples for metals only after DEP site-specific approval Filter before chemical preservation or chilling FS 2000 Collecting VOC Samples Teflon-coated silicone cap liner (septum) Prevent contamination from fuel sources Do not open VOC vial until ready to fill No aeration or agitation of sample Reduce flow from pumps or faucets to 100 mL/min Dechlorinate per SOP instructions and restrictions Do not overfill pre-preserved vials Do not pour sample into vial cap No headspace in VOC vial after collection Do not re-open vials to refill or to remove bubbles ( 5mm total bubble diameter OK) FS 2004 Sampling for Bacteria Collect last (short holding time) Sterilized containers required Use dechlorinating agent (if applicable) Thoroughly rinse any intermediate devices Keep fingers away from container opening Don’t underfill or overfill whirlpaks Close whirlpaks securely Cool immediately with wet ice FS 2005 Sampling for Hydrocarbons (Oil &Grease, TRPH, FL-PRO) Do not skim surface of water (unless required by sampling plan) Do not pre-rinse bottles with sample Do not use automatic samplers Preserve with sulfuric or hydrochloric acid and chill FS 2006 FS 2100 Surface Water FS 2400 Wastewater Common Procedures General Cautions Equipment Selection Grab and Composite Definitions Sampling Techniques Surface & Depth Grab Samples Manual or Automatic Composite Samples Sampling Strategies Collect Least to most contaminated Downstream to upstream Bow of the boat Upwind and away from fuel sources Upstream and away from body Don’t suspend bottom material into water column Sample water before collecting sediments Consider: Location relative to artificial structures Permit-specific sampling points Flow characteristics of sample source Heterogeneity or homogeneity of sample source FS 2100 & FS 2400 Representative Sampling and Contamination Prevention Mix discrete sub-samples thoroughly prior to manual compositing Avoid cross-contamination between sampling points Clean reusable equipment appropriately Collect field QC blanks as applicable Flush wastewater taps to remove settled solids and reduce flow before sampling Dechlorinate wastewater samples when required VOCs, extractable organics, microbiology, cyanide FS 2100 & FS 2400 Grab Samples Use As: Discrete sample collected over a period of time not exceeding 15 minutes Snapshot in time Direct observation of conditions at time of sampling Intermittent flows Constant waste stream or water body characteristics Batch discharges Determining min/max concentrations Tracing slug loads or spills Analytes with short holding times Analytes altered by composite techniques FS 2110, FS 2420 & FS 2430 Using a Sample Container Do not use pre-preserved containers Use intermediate sampling device in this case Sample is collected directly into sample container by hand Pole extensions may be used Collect surface samples within top 12” of water column Sampling plan may require greater depth Avoid skimming unless required by sampling plan Direct container grab preferred over intermediate devices FS 2110 & FS 2430 Using Intermediate Devices Restrictions: Avoid O&G, TRPH, FL-PRO Appropriate construction materials Rinse with site water – Discard rinses away from & downstream of sample location FS 2100 & FS 2430 Grab Samples at Depth Equipment selection is dependent on project objectives and analyte groups Water column depth is measured and recorded Sampling point depth is accurately determined and recorded Bottom sediments are not disturbed FS 2110 & FS 2430 Using Pumps Proper configuration and construction materials Do not collect O&G, TRPH & FL-PRO Do not pull organics through peristaltic pump head Flush pump and tubing with 3 volumes of sample water Anchor intake at calculated depth Record depth of intake FS 2110, & FS 2430 Composite Sample: A sample collected over time, taken either by continuous sampling or by mixing discrete samples. Composite samples reflect the average characteristics during the compositing period Use if: Continuous flow Limited analytical capacity Average analyte concentration over time Calculating mass/unit time loadings Correlation of concentration data with flow Reduction of sampling errors and hazard exposure with use of autosamplers Unattended sampling with autosamplers FS 2110, FS 2410, FS 2423 & FS 2430 Groundwater General Considerations: Fresh Formation (aquifer) Water Must be Collected Amount of Water Purged Determined by Well Conditions Parameter-driven Completion Criteria Bailers Not Recommended Specific Techniques Must be used FS 2200 General Purging Procedures Avoid dropping anything to the bottom of well Ensure proper pump or tubing placement in well and record placement depth Measure water level frequently during purging and stabilize drawdown with pump rate Purge required minimum volumes Measure parameters at proper frequency Satisfy purge completion criteria FS 2213 Purging with a Pump Place Intake at Correct Depth Stabilize Drawdown with Pump Rate Purge Required Minimum Volumes Measure Parameters at Proper Frequency Satisfy Purge Completion Criteria Conventional Purge To Pump Intake placed at the top of water column Water Level Purge at least 1½ well volumes Intake Screened Interval Calculate well volume Purge one well volume Measure stabilization parameters no sooner than each ¼ well volume interval Minimized Purge Volumes To Pump Well screen length 10 feet Configuration used for both purging and sampling Intake or pump placed at midscreen Calculate equipment volume Purge one equipment volume Measure stabilization parameters Collect additional measurements no sooner than every 2 minutes Water Level Purge a minimum of 3 equipment volumes Screened 10 ft Interval Intake FS 2212 & FS 2213 Partially Submerged Screen To Pump Intake placed at ½ distance between top of water column and bottom of screen Calculate well volume Purge one well volume Measure stabilization parameters Collect additional measurements no sooner than every 2 minutes Water Level Screened Interval Intake Purge a minimum of 1 Well Volume FS 2212 & FS 2213 Use a Flow-Through Cell Purging Completion Criteria Three consecutive Measurements Temperature: ± 0.2° C pH: ± 0.2 Standard Units Specific Conductance: ± 5.0% difference Dissolved Oxygen: 20% Saturation Turbidity: 20 NTU or Three Consecutive Measurements: Temperature: ± 0.2° C pH: ± 0.2 Standard Units Specific Conductance: ± 5.0% difference Dissolved Oxygen: ± 0.2 mg/L or ±10%, whichever is greater Turbidity: ± 5 NTU or ±10%, whichever is greater FS 2212 Maximum (Default) Purge Volumes Five Well Volumes only if Stabilization Measurements Fail Proper Techniques Verified Project Manager Approval One Fully Dry Purge (only for low recharge at < 100 mL/min pump rate) Purging and Sampling Low-Recharge Wells Minimize equipment volume Use thick-walled tubing Use same equipment for purging and sampling Place intake at mid-screen depth Purge < 100 mL/min Purge 2 equipment volumes Measure parameters just before sampling Keep pump at the purge position Keep pumping rate the same (unless purged dry) Collect samples immediately after purging (or after dry recharge if purged at < 100 mL/min) FS 2212 & FS 2222 Maximum Time Between Purging & Sampling Re-measure parameters > 1 hour after purging Re-purge if parameters > 10% of original Dry-purge wells sampled immediately after sufficient recharge & parameter measurement All samples collected within 6 hours of purging FS 2212 Purging & Sampling Wells with Installed Plumbing Purge System until Stable (pH, DO, Turbidity, Temperature and Specific Conductance) Reduce flow to 500 ml/min ( 100 mL/min for VOCs) Collect samples directly into sample containers FS 2214, FS 2215 & FS 2223 Sediment Sampling Base equipment selection on sample type and analytes of interest Sampling locations should allow a representative portion of the sediment to be collected with minimal disturbance Collect surface water samples before beginning sediment sampling FS 4000 Scoops Most useful around the margin or shore of the water body or by wading in shallow waters. Take precautions not to disturb the bottom prior to scooping. Scoop the sample in the upstream direction of flow. There are ways to modify scoops. FS 4000 Cores Use only appropriate construction materials for corers and liners. Cores preserve historical sediment layering. Water displacement is minimal (minimizes the shock wave produced by other equipment such as dredges). Good for sampling fine sediments in static waters, especially those containing trace organics and metals. Sample washout can be a problem: prevent! Nosepiece or core-catcher at bottom Check-valve at top FS 4000 Dredges Make sure the jaws fully close! Ponar - hard or rocky substrates, in deep water bodies, or streams with fast currents Eckman - soft, mucky sediments Ponar Eckman Peterson FS 4000 Soil Sampling Select sampling equipment based on the type of sample to be collected and the analytes of interest. Choose locations such that a representative portion of the soil is collected with minimal disturbance. Locations where stressed or dead natural vegetation and/or areas with surficial soil staining may indicate improper waste disposal practices. Attempt to match background sample soil types (e.g., sands vs. clays) with the site soil characteristics. FS 3000 Composite Sampling Composite soil samples in the laboratory, not the field. Soil composites depend on the project DQOs Design composite scheme per project objectives Select sampling points for each aliquot or subsample Record the amount of each aliquot Recommended: Do not mix sample aliquots before containerizing Request mixing of unmixed composite sub-samples by lab FS 3000 Surface Soil Sampling Surface soil Soil between the ground surface and 6-12 inches below ground surface Remove leaves, grass and surface debris from the area to be sampled. Select an appropriate precleaned sampling device to collect the sample. FS 3000 Subsurface Sampling Interval begins at approximately 12 inches below ground surface. DEP Recommended (resulting in minimal disturbance to the sample): Core Barrel Split Spoon Sampler Other methods: Shovels and Diggers Backhoe Bucket Augers and Hollow Corers Direct Push Shelby Tube Sampler FS 3000 FT 1000 – FT 3000 Field Testing pH Specific Conductance Temperature Dissolved Oxygen Turbidity Residual Chlorine Water Flow Sediment Oxygen Demand Dissolved Gases Salinity Transparency Continuous Monitoring Sulfite Aquatic Habitat Characterization ORP Combustible Gases General Field Testing Requirements Measurement Concepts Initial calibration of instruments Verification per acceptance criteria “Bracketing” sample tests with verifications General Requirements for Calibrations General Requirements for Verifications Documentation Requirements Calibrations Verifications Sample Measurements FT 1000 Field Testing Concepts Calibration: Manually or Automatically Adjusting Electronics Measure Calibration Calibrate (Adjust) Instrument Standard Display Value with Instrument Calibrations can only occur at the beginning of a chronological bracket FT 1000 Field Testing Concepts Verification: Checking the Instrument with Known Standard Value to Verify Instrument Response Not Acceptable Measure a Standard as if it was a Sample Compare Result with Acceptance Criterion Accept or Reject Verification Acceptable Meter Readings FT 1000 Field Testing - Bracketing Chronological Bracket Initial calibration = 10:00 AM Sample measurement = 10:15 AM Calibration verification = 10:30 AM Quantitative Range Bracket Standard A: pH = 7.0 Standard B: pH = 10 pH in Sample: = 7.5 Standard A: 100 umhos/cm Standard B: 1000 umhos/cm Sample = 400 umhos/cm FT 1000 Out of Quantitative Bracket Range Standard A: pH = 7.0 Standard B: pH = 10 pH in Sample: = 6.5 Standard A: .18 mg/L Cl Standard B: 1.5 mg/L Cl Sample = 3 mg/L Cl Report any Sample Values that are Outside the Range of the Standards as Estimated (“J”) or Verify Calibration with Additional Standard to Include Sample in Quantitative Bracket: Standard C: pH = 4.0 Standard C = 4 mg/L Cl FT 1000 Calibration/Verification/Measurement Sequence “Calibrate” Mode Begin with a Standard Calibrate or adjust instrument Calibrate or adjust instrument Choose 2nd Standard “Read” (Verification) Mode Measure Verification Standard Verification acceptable? YES Verify with different Standard Out of Standard Range? Measure samples NO Repeat Verification Or Perform Cal. Calibration Verification Frequency Perform Verification Before Measuring Samples Perform Verification at End of Use or at 24hour Intervals (initially) Extended Verification Intervals up to One Month are Acceptable if Instrument Stability is Documented Temperature Verifications Can Be Further Extended FT 1000 Secondary Standards For Chlorine and Turbidity Typically Manufacturer -specific Field-rugged for Routine use Store Upright Protect from Sun Protect from Extreme Temperatures Discard when no longer verifiable FT 1000 Verifying Secondary Standards Use Same Instrument Use only Standards that meet Manufacturer’s and SOP requirements Verify at least Quarterly Verify Using Primary Standard(s) Include a Blank for TRC “READ” Each Secondary Standard You may need to assign values the first time you verify secondary standards. - Read and follow the mfg instructions Instrument Value must be within SOP-Specified Range of the Stated or Assigned Value and The Manufacturer’s Tolerance Range Only Use Secondary Standards for Verification FT 1600 & 2000 Continuous Monitoring Devices In-Line Instrument Calibrate: Before First Installation When Verifications are not acceptable Verify After Every Calibration After Preventive Maintenance Activities or When Taken Off line Use Primary Standards FT 1900 Continuous Monitoring Devices Indirect Verification Verifications Perform at least monthly or At Intervals to ensure Continuing Acceptance Collect Sample at Same Point that Instrument Sensor is Deployed Analyze Sample using Selected Test Method Compare Results with Monitor Reading Value must be within SOP Verification Requirements FT 1900 Checking Your Technique Dissolved Oxygen (FT 1500) Vent to atmosphere Inspect membrane Enclose with saturated air Ensure stable temperature Checking Your Technique Dissolved Oxygen Verification (FT 1500) Record Temperature Record DO in mg/L Table FS 2200-2 Dissolved Oxygen Saturation TEMP D.O. deg C SAT. 15.0 15.1 15.2 15.3 15.4 15.5 15.6 15.7 15.8 15.9 16.0 16.1 16.2 16.3 16.4 16.5 16.6 16.7 16.8 16.9 17.0 17.1 17.2 mg/L TEMP 20% deg C D.O. SAT. mg/L TEMP 20% deg C D.O. SAT. mg/L TEMP 20% deg C D.O. mg/L SAT. 20% 10.084 2.017 19.0 9.276 1.855 23.0 8.578 1.716 27.0 7.968 1.594 10.062 2.012 19.1 9.258 1.852 23.1 8.562 1.712 27.1 7.954 1.591 10.040 2.008 19.2 9.239 1.848 23.2 8.546 1.709 27.2 7.940 1.588 10.019 2.004 19.3 9.220 1.844 23.3 8.530 1.706 27.3 7.926 1.585 9.997 1.999 19.4 9.202 1.840 23.4 8.514 1.703 27.4 7.912 1.582 9.976 1.995 19.5 9.184 1.837 23.5 8.498 1.700 27.5 7.898 1.580 9.955 1.991 19.6 9.165 1.833 23.6 8.482 1.696 27.6 7.884 1.577 Table 1: Solubility of Oxygen in Water 9.934 1.987 19.7 9.147 1.829 23.7 8.466 1.693 27.7 7.870 1.574 9.912 1.982 19.8 9.129 1,2 1.826 23.8 8.450 1.690 27.8 7.856 1.571 at Atmospheric Pressure 9.891 1.978 19.9 9.111 1.822 23.9 8.434 1.687 27.9 7.842 1.568 9.870 1.974 20.0 9.092 1.818 24.0 8.418 1.684 28.0 7.828 1.566 9.849 1.970 20.1 9.074 1.815 24.1 8.403 1.681 28.1 7.814 Temperature Oxygen Solubility Temperature Oxygen Solubility 1.563 9.829 1.966 20.2 9.056 1.811 24.2 8.387 1.677 28.2 7.800 1.560 o o C 20.3 9.039mg/L C 1.674 28.3mg/L 9.808 1.962 1.808 24.3 8.371 7.786 1.557 9.787 1.957 20.4 9.021 1.804 24.4 8.356 1.671 28.4 7.773 1.555 0.0 14.621 26.0 8.113 9.767 1.953 20.5 9.003 1.801 24.5 8.340 1.668 28.5 7.759 1.552 1.0 20.6 8.985 14.216 27.0 1.665 28.67.968 9.746 1.949 1.797 24.6 8.325 7.745 1.549 9.726 1.945 1.794 24.7 8.309 7.732 1.546 2.0 20.7 8.968 13.829 28.0 1.662 28.77.827 9.705 1.941 20.8 8.950 1.790 24.8 8.294 7.718 1.544 3.0 13.460 29.0 1.659 28.87.691 9.685 1.937 20.9 8.932 1.786 24.9 8.279 1.656 28.9 7.705 1.541 4.0 21.0 8.915 13.107 30.0 1.653 29.07.559 9.665 1.933 1.783 25.0 8.263 7.691 1.538 9.645 1.929 1.780 25.1 8.248 7.678 1.536 5.0 21.1 8.898 12.770 31.0 1.650 29.17.430 9.625 1.925 21.2 8.880 1.776 25.2 8.233 1.647 29.2 7.664 1.533 Compare with Chart (Table FT 1500-1 or FS 2200-2) 60 12 447 32 0 7 305 Table FS 2200-2 Dissolved Oxygen Saturation Temperature: 21.6 Reading: 9.08 Theoretical: 8.812 Difference: 0.268 TEMP D.O. D.O. mg/L deg C SAT. mg/L TEMP 20% deg C SAT. D.O. mg/L TEMP 20% deg C SAT. D.O. mg/L TEMP 20% deg C SAT. 20% 15.0 15.1 15.2 15.3 15.4 15.5 15.6 15.7 15.8 15.9 16.0 16.1 16.2 16.3 16.4 16.5 16.6 16.7 16.8 16.9 17.0 17.1 17.2 17.3 17.4 17.5 17.6 17.7 17.8 17.9 18.0 18.1 18.2 18.3 18.4 18.5 18.6 18.7 18.8 18.9 10.084 10.062 10.040 10.019 9.997 9.976 9.955 9.934 9.912 9.891 9.870 9.849 9.829 9.808 9.787 9.767 9.746 9.726 9.705 9.685 9.665 9.645 9.625 9.605 9.585 9.565 9.545 9.526 9.506 9.486 9.467 9.448 9.428 9.409 9.390 9.371 9.352 9.333 9.314 9.295 2.017 2.012 2.008 2.004 1.999 1.995 1.991 1.987 1.982 1.978 1.974 1.970 1.966 1.962 1.957 1.953 1.949 1.945 1.941 1.937 1.933 1.929 1.925 1.921 1.917 1.913 1.909 1.905 1.901 1.897 1.893 1.890 1.886 1.882 1.878 1.874 1.870 1.867 1.863 1.859 19.0 19.1 19.2 19.3 19.4 19.5 19.6 19.7 19.8 19.9 20.0 20.1 20.2 20.3 20.4 20.5 20.6 20.7 20.8 20.9 21.0 21.1 21.2 21.3 21.4 21.5 21.6 21.7 21.8 21.9 22.0 22.1 22.2 22.3 22.4 22.5 22.6 22.7 22.8 22.9 9.276 9.258 9.239 9.220 9.202 9.184 9.165 9.147 9.129 9.111 9.092 9.074 9.056 9.039 9.021 9.003 8.985 8.968 8.950 8.932 8.915 8.898 8.880 8.863 8.846 8.829 8.812 8.794 8.777 8.761 8.744 8.727 8.710 8.693 8.677 8.660 8.644 8.627 8.611 8.595 1.855 1.852 1.848 1.844 1.840 1.837 1.833 1.829 1.826 1.822 1.818 1.815 1.811 1.808 1.804 1.801 1.797 1.794 1.790 1.786 1.783 1.780 1.776 1.773 1.769 1.766 1.762 1.759 1.755 1.752 1.749 1.745 1.742 1.739 1.735 1.732 1.729 1.725 1.722 1.719 23.0 23.1 23.2 23.3 23.4 23.5 23.6 23.7 23.8 23.9 24.0 24.1 24.2 24.3 24.4 24.5 24.6 24.7 24.8 24.9 25.0 25.1 25.2 25.3 25.4 25.5 25.6 25.7 25.8 25.9 26.0 26.1 26.2 26.3 26.4 26.5 26.6 26.7 26.8 26.9 8.578 8.562 8.546 8.530 8.514 8.498 8.482 8.466 8.450 8.434 8.418 8.403 8.387 8.371 8.356 8.340 8.325 8.309 8.294 8.279 8.263 8.248 8.233 8.218 8.203 8.188 8.173 8.158 8.143 8.128 8.114 8.099 8.084 8.070 8.055 8.040 8.026 8.012 7.997 7.983 1.716 1.712 1.709 1.706 1.703 1.700 1.696 1.693 1.690 1.687 1.684 1.681 1.677 1.674 1.671 1.668 1.665 1.662 1.659 1.656 1.653 1.650 1.647 1.644 1.641 1.638 1.635 1.632 1.629 1.626 1.623 1.620 1.617 1.614 1.611 1.608 1.605 1.602 1.599 1.597 27.0 27.1 27.2 27.3 27.4 27.5 27.6 27.7 27.8 27.9 28.0 28.1 28.2 28.3 28.4 28.5 28.6 28.7 28.8 28.9 29.0 29.1 29.2 29.3 29.4 29.5 29.6 29.7 29.8 29.9 30.0 30.1 30.2 30.3 30.4 30.5 30.6 30.7 30.8 30.9 7.968 7.954 7.940 7.926 7.912 7.898 7.884 7.870 7.856 7.842 7.828 7.814 7.800 7.786 7.773 7.759 7.745 7.732 7.718 7.705 7.691 7.678 7.664 7.651 7.638 7.625 7.611 7.598 7.585 7.572 7.559 7.546 7.533 7.520 7.507 7.494 7.481 7.468 7.456 7.443 1.594 1.591 1.588 1.585 1.582 1.580 1.577 1.574 1.571 1.568 1.566 1.563 1.560 1.557 1.555 1.552 1.549 1.546 1.544 1.541 1.538 1.536 1.533 1.530 1.528 1.525 1.522 1.520 1.517 1.514 1.512 1.509 1.507 1.504 1.501 1.499 1.496 1.494 1.491 1.489 Derived using the formula in Standard Methods for the Examination of Water and Wastewater, Page 4-101, 18th Edition, 1992 Checking your Technique pH (FT 1100) When Calibrating, always begin with a pH 7 buffer Rinse Probe with DI Water between Measurements Check your Technique Turbidity (FT 1600) Only Calibrate a Turbidity-free Water Blank and Primary Standards Formazin or Styrene divinylbenzene Verify Calibration with Primary Standard Verify Secondary Gel Standards Quarterly Verify with a Primary Standard before “reading” the Gel Standards Check your Technique Specific Conductance (FT 1100) Select Standards that bracket the range of sample measurements A 100 umho/cm standard is acceptable verification for any sample below 100 umho/cm Calibration Records Standards Manufacturer’s Name Lot Number Expiration Date Concentration Range (if applicable) FT 1000 Calibration Records Dissolved Oxygen Verification reading (mg/L) Verification temperature (C) Expected saturation value (mg/L) Calculated Deviation from Expected Saturation Value FT 1000 Calibration Records pH and Specific Conductance pH Calibration buffer values Verification buffer expected value Meter reading of verification buffer Calculated deviation from expected value Specific Conductance Calibration standard value Verification standard expected value Meter reading of verification standard Calculated % deviation from expected value FT 1000 Calibration Records Turbidity & Residual Chlorine Primary Calibration Standards Standard Values Expected Value(s) of Primary Standard used for Verification Instrument Reading of Verification Standard(s) Calculated % Deviation of Readings from Expected Value(s) FT 1000 Calibration Records Turbidity & Residual Chlorine Records when Assigning Values or Verifying Secondary Standards Previously Assigned 2ndary Standard Value or Manufacturer’s Stated Value Current Reading from Instrument Calculated Deviation between Current Reading and Previous Value New Value assigned from Current Reading (if applicable) Manufacturer’s Tolerance Range (for Residual Chlorine) FT 1000 Calibration Records Turbidity & Residual Chlorine Records when Verifying with Secondary Standards Verification Standard Value(s) (expected values) Reading from 2ndary Standard(s) Calculated % Deviations from Expected Verification Values FT 1000 Field Testing Acceptance Criteria per DEP-SOP-001/01 Parameter Acceptance Criteria pH + 0.2 Standard pH Units of calibration buffer Specific Conductance + 5% of calibration standard value Dissolved Oxygen + 0.3 mg/L of theoretical value Temperature NIST-traceable value (with correction factors) Verification over range of sample values Turbidity 0.1-10 NTU: + 10% of the calibration standard 11-40 NTU: + 8% of the calibration standard 41-100 NTU: + 6.5% of the calibration standard > 100 NTU: + 5% of the calibration standard Total Residual Chlorine 0.995 calibration curve correlation coeffecient + 10% of primary calibration standard value + 10% of secondary standard value eNewsletter (automatic updates on QA topics & training) http://www.dep.state.fl.us/labs/training/listserve.htm Environmental Assessment Section 2600 Blair Stone Road MS 6511 Tallahassee, FL 32399-2400 SC 205 8065 (850) 245-8065 Contacts: firstname.lastname@dep.state.fl.us Russel Frydenborg, Administrator Silky Labie Michael Blizzard Thomas Deck Denise Miller Thomas Frick Andrew Tintle Jessica Patronis Joyce Ziesenis
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