1. No category

Sample Handling
SPE & Filtration .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..1-39
SPE Introduction .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 2-5
Drug-Clean™ SPE .. .. .. .. .. .. .. .. .. .. .. .. .. 6-7
Ultra-Clean™ SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. 8
Maxi-Clean™ SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. 9
Reversed-Phase SPE .. .. .. .. .. .. .. .. .. .. 10-13
Normal-Phase SPE .. .. .. .. .. .. .. .. .. .. .. 14-16
Specialty SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 17
Ion Exchange & IC SPE .. .. .. .. .. .. .. .. .. 18-21
SPE Accessories .. .. .. .. .. .. .. .. .. .. .. .. 22-26
Filtration Introduction
.. .. .. .. .. .. .. .. .. .. .. 27-29
Syringe Filters .. .. .. .. .. .. .. .. .. .. .. .. .. .. 30-35
Other Filters & Accessories .. .. .. .. .. .. .. 36-39
Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 40-54
Derivatization Reagents .. .. .. .. .. .. .. .. .. .. 40-44
Ion-Pair Reagents .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 45
Gas Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 46-48
Drug Standards
.. .. .. .. .. .. .. .. .. .. .. .. .. .. 49-52
FAME & Hydrocarbon Standards
.. .. .. .. .. 53-54
Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 55-71
Introduction
.. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 55
Hamilton Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. 56-64
Popper Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 65
VICI-Precision Syringes .. .. .. .. .. .. .. .. .. .. 66-67
SGE Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 68-70
Pipetors & Dispensers .. .. .. .. .. .. .. .. .. .. .. .. .. 71
Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 72-122
Introduction
.. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 72-77
12 x 32mm Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. 78-92
8 x 40mm Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 93
15 x 45mm Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. 94-97
Specialty Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. 98-103
Storage Vials, EPA Vials .. .. .. .. .. .. .. .. ..104-113
96-Well Products .. .. .. .. .. .. .. .. .. .. .. .. ..114-117
© Copyright 2004 Alltech Associates, Inc.
Accessories
.. .. .. .. .. .. .. .. .. .. .. .. .. .. ..118-121
Vial Crusher
.. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 122
Contact your Alltech office or distributor for current or local prices.
1
SPE
SPE Introduction
Choosing an SPE Product
1. Characterize the sample. Factors such
as the analyte’s polarity relative to the
matrix, the presence of charged functional
groups, solubility, molecular weight, etc.,
determine how strongly the analyte is
retained by the packed bed.
2. Select a retention strategy. There are
two basic methods for sample treatment:
a. Select the packing bed to retain the
desired analyte. The contaminants
are washed off and the desired analyte
is then eluted for analysis.
b. Select the packing bed to retain the
contaminants and the desired analyte
passes directly through.
4914
3. Select the proper packing type for the cleanest extract with the highest recovery.
a. Reversed-phase packings are hydrophobic, silica-based materials that retain moderately polar to nonpolar compounds from a polar matrix while washing off polar interferences. Or you can retain non-polar
contaminants while the polar compounds pass through unretained.
b. Normal-phase packings are hydrophilic, silica-based materials that retain polar compounds from a
non-polar matrix while washing off non-polar interferences. Or you can retain polar contaminants while
non-polar compounds pass through unretained.
c. Ion-exchange resins retain charged compounds or remove ionic interferences.
4. Optimize conditions for best results. Select proper bed size and suitable conditioning, wash and elution
solvents.
a. Poor sample recovery often occurs when the packed bed dimensions are not optimized. Too large a bed
results in incomplete elution while too small a bed results in incomplete retention.
b. Consider the solvent strength relative to the packing material. The final conditioning solvent should
be weak so it doesn’t act as an eluting solvent. Buffers should be used to control ionization of
potentially charged compounds.
c. Wash solvents should remove weakly retained interferences without being strong enough to elute the
analyte.
d. Elution solvents should be strong enough to completely elute an analyte in a small volume (1-2mL).
For Environmental Applications
Recommended SW-846 Cleanup Methods
EPA
ANALYTE GROUP
METHOD NO.
Aniline and Derivatives
3620
Chlorinated Pesticides
3620
Chlorinated Hydrocarbons
3620
Haloethers
3620
Nitroaromatics and Cyclic
3620
Ketones
Nitrosamines
3610, 3620
Organochlorine Pesticides
3620
Organophosphorus Pesticides
3620
PCB’s
3620, 3630
Petroleum Waste
3611
Phenols
3630
Phthalate Esters
3610, 3620
Polynuclear Aromatic
3630
Hydrocarbons
© Copyright 2004 Alltech Associates, Inc.
2
RECOMMENDED
SPE SORBENTS
C18
C18, Florisil®
C18, Phenyl
C18, Florisil®
DVB, IC-RP
Need Technical Assistance?
Call Alltech Tech Support:
Email: tech_service@alltechemail.com
Online: www.alltechWEB.com
C18
C18, Florisil®
Florisil®, Carbograph
C18, SCX, Carbograph
Alumina
C18, Phenyl
C18, DVB
C18, Phenyl
Contact your Alltech office or distributor for current or local prices.
SPE
SPE Introduction
SPE Sorbent Specifications
SPE Sorbent Specifications
FUNCTIONAL GROUP
BASE
% CARBON ENDCAPPED
Reversed-Phases (Non-Polar)
Prevail C18
Silica
11.0%
Yes
Octadecyl (C18)
Silica
6.0%
Yes
High Flow C18
Silica
8.0%
Yes
High Capacity C18
Silica
17.0%
Yes
Large Pore C18
Silica
14.0%
Yes
Oil & Grease C18
Silica
17.0%
Yes
Octyl (C8)
Silica
4.5%
Yes
High Capacity C8
Silica
8.5%
Yes
Ethyl (C2)
Silica
5.5%
Yes
Phenyl (PH)
Silica
3.8%
Yes
Cyclohexyl (CH)
Silica
3.5%
Yes
Normal-Phases (Polar)
Silica (SI)
Silica
—
—
Aminopropyl (NH2)
Silica
5.0%
No
Cyanopropyl (CN)
Silica
6.0%
Yes
Diol (2OH)
Silica
4.0%
No
Florisil® (FL) (FL-PR) Magnesium Silicate
—
—
Alumina (AL)
Aluminum Oxide
—
—
Specialty Packings
DVB
100% DVB
—
—
Carbograph
Graphitized Carbon
—
—
Drug-Clean™
Silica
—
—
Drug-Clean™ PB
Polymer
—
—
AVERAGE
PARTICLE SIZE PORE SIZE
50µm
50µm
100µm
50µm
50µm
100µm
50µm
50µm
50µm
50µm
50µm
60Å
60Å
60Å
60Å
150Å
60Å
60Å
60Å
60Å
60Å
60Å
PAGE
How to Use Solid Phase
Extraction Products
10
12
12
12
12
12
13
13
13
13
13
50µm
50µm
50µm
50µm
75-150µm
130µm
60Å
60Å
60Å
60Å
60Å
100Å
15
14
14
14
16
16
40µm
38-125µm
50µm
30µm
—
—
60Å
—
17
17
6
7
By syringe
4915
Ion Exchange Specifications
PACKING TYPE
SCX
SAX
IC-OH
IC-H
IC-Ag
IC-Ba
IC-Na
IC-Chelate
IC-RP
BASE
Styrene-DVB
Styrene-DVB
Styrene-DVB
Styrene-DVB
Styrene-DVB
Styrene-DVB
Styrene-DVB
Styrene-DVB
Polystyrene
COUNTER ION
Hydrogen
Acetate
Hydroxide
Hydronium
Silver
Barium
Sodium
Sodium
—
PARTICLE SIZE
35-70µm
35-70µm
35-70µm
35-70µm
35-70µm
35-70µm
35-70µm
35-70µm
250-850µm
MOLECULAR
EXCLUSION LIMIT
1000 Daltons
1000 Daltons
1000 Daltons
1000 Daltons
1000 Daltons
1000 Daltons
1000 Daltons
1000 Daltons
—
EXCHANGE
CAPACITY
2.0meq/mL
1.0meq/mL
1.0meq/mL
2.0meq/mL
2.0meq/mL
2.0meq/mL
2.0meq/mL
0.4meq/mL
—
PAGE
18
18
20
20
20
20
20
20
20
By vacuum manifold
6275
Solvent Polarity Chart
Less Polar (Stronger Reversed-Phase Elution)
Hexane
Isooctane
Cyclohexane
Carbon tetrachloride
Toluene
Chloroform
Methylene chloride
Tetrahydrofuran
Ethyl ether
Acetone
Acetonitrile
Pyridine
Isopropanol
Ethanol
Methanol
Water
By filter flask
4919
Acetic acid
More Polar (Stronger Normal-Phase Elution)
© Copyright 2004 Alltech Associates, Inc.
Contact your Alltech office or distributor for current or local prices.
3
SPE
SPE Introduction
SPE Column Formats
Actual Size Drawings
Extract-Clean™ and Ultra-Clean™ Columns
4976
1.5mL
4975
4974
8.0mL
4.0mL
Extended Volume Columns
4973
15mL
4972
25mL
Maxi-Clean™ Cartridges
4977
4978
4971
300mg Bed
600mg Bed
75mL
4979
900mg Bed
6276
10.0mL
100-500mg Bed
Device Specifications
DEVICE
Extract-Clean™ Columns
Ultra-Clean™ Columns
Extended Volume Columns
Maxi-Clean™ Cartridges
96-Well SPE Plates
© Copyright 2004 Alltech Associates, Inc.
4
HOUSING
Polypropylene
Treated Polypropylene
Polypropylene
Polypropylene
Polypropylene
FRIT MATERIAL
20µm Polyethylene
10µm PTFE
20µm Polyethylene
20µm Polyethylene
20µm Polyethylene
DESCRIPTION
For a Full Range of Sample Sizes
Inert Format for Sensitive Analyses
10mL Flared Design for Dilute Samples
Luer-Inlet Cartridges for Manual Processing
2.0mL Square Well for Automated Procedures
Contact your Alltech office or distributor for current or local prices.
SPE
SPE Introduction
General Solid-Phase
Extraction Procedures:
Extract-Clean™ Method
Development Kits
Reversed-Phase
Method Development Kits include a variety of packing
materials to rapidly identify the packing which provides
the cleanest extract and highest recovery for your
sample.
To isolate relatively non-polar compounds from a polar matrix
A. Conditioning
Rinse device with 3-5mL of methanol followed by
3-5mL of deionized water/buffer (do not allow bed to
dry before next step).
B. Sample Application
Apply sample to the top of the device and draw
through the packing bed. Flows of 1-5mL/min are
optimal.
C. Wash
Wash with 5mL of a polar solvent if analyte is to be
retained.
D. Elution
Elute analyte into a collection tube with 1-5mL of a
non-polar solvent.
Choose a Reversed-Phase Kit to retain moderately
polar to non-polar compounds from a polar matrix.
Choose a Normal-Phase Kit to retain polar compounds
from a non-polar matrix.
Complete Kits are available with reversed-phases,
normal-phases, and ion exchangers.
Normal-Phase
To isolate polar compounds from a non-polar matrix
A. Conditioning
Rinse device with 3-5mL of non-polar solvent.
B. Sample Application
Apply sample to the top of the device and draw
through the packing bed (1-5mL/min).
C. Wash
Wash with 5mL of a non-polar solvent if analyte is to
be retained.
D. Elution
Elute analyte into a collection tube with 1-5mL of a
polar solvent.
Ion-Exchange
To isolate charged or potentially charged compounds
A. Conditioning
Rinse device with 3-5mL of deionized water or low
ionic strength buffer (0.01M).
B. Sample Application
Apply sample to the top of the device and draw
through the packing bed at 1mL/min (ion exchange
kinetics are slower than reverse or normal-phase).
C. Wash
Wash with 5mL of deionized water or low ionic
strength buffer.
D. Elution
Elute analyte into a collection tube with 1-5mL of
buffer at high ionic strength (0.1-1M) or modified pH
(pH such that analyte is uncharged).
© Copyright 2004 Alltech Associates, Inc.
5493
METHOD DEVELOPMENT KITS
BED WT, COLUMN SIZE
PART NO.
Normal-Phase Kits
100mg, 1.5mL (Includes 10 columns each of
211200
Silica, Florisil®, Amino, CN, and Diol)
500mg, 4.0mL (Includes 5 columns each of
Silica, Florisil®, Amino, CN, and Diol)
PRICE
211300
Reversed-Phase Kits
100mg, 1.5mL (Includes 10 columns each of C18, 211001
C8, C2, and Phenyl)
500mg, 4.0mL (Includes 5 columns each of C18,
C8, C2, CH, and Phenyl)
211100
Complete Kits
100mg, 1.5mL (Includes 10 columns each of C18, 210100
C8, C2, Diol, CN, NH2, Silica, SAX, and SCX)
500mg, 4.0mL (Includes 5 columns each of C18,
C8, C2, CH, Diol, CN, NH2, Silica, SAX, and SCX)
210500
SPE packings only function
properly after wetting the packing
surface. The conditioning step
establishes a dynamic equilibrium
between the solvent and the packing
material. For reproducible results, do
not allow the packing to dry between
the conditioning step and addition
of the sample.
Contact your Alltech office or distributor for current or local prices.
5
Drug-Clean
™
SPE
SPE
Drug-Clean™ Mixed Mode SPE Columns
For Accurate Drug Analysis Results
• Efficient—higher recoveries with minimal impurities
compared to single mode extractions
• Multi-functional—extract acidic, neutral, and basic
compounds with a single SPE bed
• Reproducible—true copolymer is cleaner and more
accurate than mixed beds
Drug-Clean™ phases are true copolymeric packings
with both reversed-phase and ion exchange groups.
Drug-Clean™ C is a C8/SCX cation function. This is
used to retain neutral or amine containing compounds
while allowing carboxylate groups to pass through.
4886
6305
Mixed mode allows high drug recoveries with minimal
impurities compared to single mode extractions
Drug-Clean™ A is a C8/SAX anion function. In this
case the neutral or carboxylate compounds are retained
and the amine group retention is minimized.
Each box of columns includes extraction methods
for the most popular NIDA drugs of abuse. Additional
methods are available on Alltech’s website.
Cocaine and Benzoylecgonine
Extract from Urine
�
Drug Clean™ Specifications
C8/Ion Exchange
Functional Group:
50µm irregular silica, 60Å
Base:
Retention Mechanism: Mixed mode, Reversed-Phase, and Ion
Exchange
DRUG-CLEAN™ COLUMNS
BED WT./COLUMN SIZE
QTY.
PART NO.
PRICE
Drug-Clean™ C (Mixed Mode Cation) Columns
100mg/1.5mL
100/pk
207015
200mg/4.0mL
50/pk
207010
500mg/4.0mL
50/pk
207017
500mg/8.0mL
30/pk
207019
Drug-Clean™ C (Mixed Mode Cation) Extended Volume Columns
200mg/10.0mL EV
30/pk
207011
Drug-Clean™ A (Mixed Mode Anion) Columns
100mg/1.5mL
100/pk
207030
200mg/4.0mL
50/pk
207034
Drug-Clean™ A (Mixed Mode Anion) Extended Volume Columns
200mg/10.0mL EV
30/pk
207038
2656
1. Cocaine (2.5mg/mL)
2. Benzoylecgonine-TMS,
Benzoylecgonine-d3-TMS (5.0mg/mL)
�
�
� �
� �
� �� �� �� �� �������
Column:
Heliflex® AT-5ms, 30m x 0.25mm x 0.25µm
Capillary GC Column (Part No. 15807)
Temp:
225ºC (0 min hold) to 315ºC (11 min hold)
at 10ºC/min
Carrier Gas:
Helium at 0.8mL/min (28cm/sec)
Detector:
MSD at 325ºC
Sample Prep: Drug-Clean™C
Request Sheet M102 for more information about
these Drug-Clean columns.
Why is a “True Copolymer” Better Than a Mixed Bed?
By definition, a mixed mode packing contains two different functional groups on the same
sorbent. To minimize costs, two individual packings are sometimes blended together physically
and packed in a tube. This gives the effect of a mixed mode but in fact is a mixed bed packing
with batch-to-batch reproducibility dependent on the blending skills of the manufacturer. A
true mixed mode has the functional groups polymerized on the same silica base. This copolymer
treatment assures a more predictable packing performance which means more reproducible results
from tube to tube, batch to batch.
© Copyright 2004 Alltech Associates, Inc.
6
Contact your Alltech office or distributor for current or local prices.
SPE
Drug-Clean™ SPE
Drug-Clean™ PB SPE Columns
For Cleaner Extractions and More Accurate
Drug Analysis
• Stable from pH 1 to 14
• No conditioning steps required
• Faster flow rates than silica-based materials
Drug-Clean PB has a C8/SCX cation function on a
highly cross-linked styrene divinylbenzene base. The
high sorbent capacity greatly reduces the amount of
packing required for separation and makes this material
ideal for use in 96-well plates.
Each box of columns includes extraction methods
for amphetamines, opiates, cocaine/benzoylecgonine,
phencyclidine and carboxy-THC.
4886
Extract acidic, neutral, and basic compounds from a single
column.
Amphetamines Extract from Urine
Drug Clean™ PB Specifications
C8/Cation Function
Functional Group:
30µm Styrene/DVB Polymer
Base:
Retention Mechanism: Mixed mode, reversed-phase and ion
exchange
DRUG-CLEAN™ PB SPE COLUMNS
BED WT./COLUMN SIZE
QTY.
PART NO.
Extract-Clean™ Columns
30mg/1.5mL
100
250120
30mg/4.0mL
50
250130
50mg/8.0mL
50
250140
96-Well Plate
30mg per Well
ea
250150
4888
2673
1
1. Amphetamine (2.5 mg/mL)
2. Methamphetamine (2.5 mg/mL)
2
PRICE
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Min.
Column:
Temp:
Carrier Gas:
Detector:
Heliflex® AT-35, 30m x 0.25mm x 0.25µm
Capillary GC Column (Part No. 13642)
180°C to 300°C at 10°C/min
Helium at 0.7mL/min (24cm/sec)
FID at 275°C
Cocaine and Benzoylecgonine Extract from Urine
2
See pages 114-117 for collection plates and
other 96 well accessory products.
2674
1. Cocaine (2.5 mg/mL)
2. Benzoylecgonine (5.0 mg/mL)
1
Solid-Phase Extraction
Principles and Practice
This excellent textbook covers SPE
theory, method development, SPE
materials and applications.
0
2
4
Column:
SOLID-PHASE EXTRACTION PRINCIPLES AND PRACTICE
DESCRIPTION
PART NO.
Solid-Phase Extraction Principles and Practice
1983
© Copyright 2004 Alltech Associates, Inc.
PRICE
Temp:
Carrier Gas:
Detector:
6
8
10 12
14
16
18 Min.
Heliflex® AT-5ms, 30m x 0.25mm x 0.25µm
Capillary GC Column (Part No. 15807)
225°C to 315°C at 10°C/min
Helium at 0.7mL/min (25cm/sec)
FID at 325°C
Contact your Alltech office or distributor for current or local prices.
7
SPE
Ultra-Clean SPE
™
Ultra-Clean™ SPE
Ideal for Environmental, Pharmaceutical and
Other Sensitive Applications
• Treated columns with the inertness of PTFE
• PTFE frits for low extractables
• Unbreakable and less expensive than glass
PACKING
Reversed-Phase
C18
C8
No Broken Glass!
Normal-Phase
Silica
Florisil®
Florisil®-PR
Aminopropyl
Carbograph
6197
Ion-Exchange
SCX
SAX
ULTRA-CLEAN™ SPE COLUMNS
BED
COLUMN
WEIGHT
SIZE
QTY. PART NO.
PRICE
200mg
500mg
200mg
500mg
500mg
4.0mL
8.0mL
4.0mL
4.0mL
8.0mL
50
30
50
50
30
505150
505355
506151
506251
506351
200mg
500mg
5000mg
500mg
1000mg
500mg
1000mg
200mg
500mg
150mg
300mg
4.0mL
8.0mL
25mL
4.0mL
8.0mL
4.0mL
8.0mL
4.0mL
4.0mL
4.0mL
8.0mL
50
30
20
50
30
50
30
50
50
50
30
509150
509225
509010
507851
507930
504651
507900
511025
511150
510142
510101
200mg
500mg
200mg
500mg
4.0mL
4.0mL
4.0mL
4.0mL
50
50
50
50
509826
509951
509626
509751
See page 3 for packing specifications.
Compare Ultra-Clean™ to Glass!
10025-27
Column:
Alltima™ C18, 5µm, 150 x 4.6mm
HPLC Column (Part No. 88052)
Mobile Phase: A: Water B: Acetonitrile
Gradient:
Time: 0 2 20
%B:
5 5 70
Flow Rate:
1mL/min
Detector:
UV at 210
10028-30
Column:
Alltima™ C18, 5µm, 150 x 4.6mm
HPLC Column (Part No. 88052)
Mobile Phase: A: Water B: Acetonitrile
Gradient:
Time: 0 2 20
%B:
5 5 70
Flow Rate:
1mL/min
Detector:
UV at 210
No new contaminants are introduced by Ultra-Clean™ SPE columns when compared to glass SPE columns.
© Copyright 2004 Alltech Associates, Inc.
8
Contact your Alltech office or distributor for current or local prices.
SPE
Maxi-Clean™ Cartridges
Maxi-Clean™ Cartridges
The Flexible Cartridge Format Saves Time
and Maximizes Your Sample Preparation
Efficiency
• Adaptable—Same bed dimensions as 4mL SPE
columns for method cross-over; Process a single
cartridge by syringe or multiple cartridges by vacuum
• Versatile—Stack different cartridges for multistep extractions; Use top and bottom caps for easy
transport of field samples
MAXI-CLEAN™ REVERSED-PHASE CARTRIDGES
PACKING
BED WEIGHT QTY.
PART NO.
Prevail™ C18
300mg
50
605926
500mg
50
605929
900mg
50
605942
C18
300mg
25
20924
300mg
50
20926
300mg
100
20928
600mg
25
20932
600mg
50
20934
600mg
100
20936
900mg
25
20940
900mg
50
20942
900mg
100
20944
High Capacity C18
300mg
50
20945
Large Pore C18
300mg
100
22012
600mg
100
22017
900mg
100
220215
C8
300mg
50
20950
600mg
50
20958
900mg
50
20966
C2
300mg
50
210064
Phenyl
300mg
25
22002
300mg
50
22003
300mg
100
22004
MAXI-CLEAN™ DRYING CARTRIDGES
PACKING
BED WEIGHT QTY.
PART NO.
Sodium Sulfate
3g
100
219001
PRICE
PRICE
4881
4915
MAXI-CLEAN™ NORMAL-PHASE CARTRIDGES
PACKING
BED WEIGHT QTY.
PART NO.
Silica
300mg
25
20972
300mg
50
20974
300mg
100
20976
600mg
25
20980
600mg
50
20982
600mg
100
20984
900mg
25
20988
900mg
50
20990
900mg
100
20992
Florisil®
300mg
50
210054
300mg
100
210056
900mg
50
210057
900mg
100
210061
Florisil®-PR
300mg
50
210074
300mg
100
210076
900mg
50
210079
900mg
100
210075
Alumina, Basic
300mg
25
210093
1800mg
25
210096
Alumina, Acidic
300mg
25
210094
1800mg
25
210097
Alumina, Neutral
300mg
25
210095
1800mg
25
210098
Diol
300mg
50
210084
300mg
100
210086
Cyanopropyl
300mg
50
210034
300mg
100
210036
Aminopropyl
300mg
50
210044
300mg
100
210046
900mg
100
210047
PRICE
What is a Maxi-Clean™ Cartridge?
A Maxi-Clean™ cartridge is an alternative format for SPE. It uses the same high quality packing
materials as the Extract-Clean™ columns but has a polypropylene housing with both a female luer
inlet and a male luer outlet tip. This allows use of positive pressure from a syringe or negative
pressure from a vacuum manifold. 20µm polyethylene frits are placed at each end of the sorbent
bed. The packing material is packed and compressed to improve or optimize flow characteristics.
For ion-exchange SPE cartridges see pages 20-21.
© Copyright 2004 Alltech Associates, Inc.
For packing specifications see page 3.
Contact your Alltech office or distributor for current or local prices.
9
SPE
Reversed-Phase SPE
Prevail™ SPE – A Universal C18
• Saves Time—Since it does not require
preconditioning with an organic solvent, it eliminates
one step from typical SPE procedures.
• Saves Solvents—Eliminating organic preconditioning
reduces handling and disposal of expensive,
hazardous organic solvents.
• Universal Applications—Prevail™ C18 for SPE is
useful for the same compounds and sample types as a
traditional C18 phase.
Prevail™ SPE Specifications
Functional Group:
Base:
Percent Carbon Loading:
Retention Mechanism
Standard C18
Shows No
Retention
Prevail™ C18
Shows Excellent
Retention
6293
2mL Methylene Blue solution
through dry, unconditioned beds
Modified C18
50µm irregular silica, 60Å
11%
Reversed-phase
Dr. Peaks says,
Prevail™ SPE Columns are ideal for:
• Drug Metabolites from Blood
• Hormones from Body Fluids
• Nutraceuticals from Foods & Beverages
• Pesticides from Water
Extract-Clean™ SPE Columns
• Larger bed weights
• Process by vacuum
4886red
BED WEIGHT
100mg
130mg
500mg
500mg
1000mg
PREVAIL™ C18 EXTRACT-CLEAN™ COLUMNS
COLUMN SIZE
QTY.
PART NO.
1.5mL
100
605001
4.0mL
50
605130
4.0mL
50
605250
8.0mL
30
605350
8.0mL
30
605430
Caffeine in Coffee
PRICE
�
Unextracted
10136
1. Caffeine
Column:
Prevail™ C18, 5µm, 150 x 4.6mm
HPLC Column (Part No. 99208)
Mobile Phase: Water:Acetonitrile (70:30)
Flow Rate:
1.0mL/min
Detector:
UV at 254
Maxi-Clean™ SPE Cartridges
• Small and portable
• Process by vacuum or syringe
�
�
�
�
�
������
�
Extracted
4882red
PREVAIL™ C18 MAXI-CLEAN™ CARTRIDGES
BED WEIGHT
QTY.
PART NO.
300mg
50
605926
500mg
50
605929
900mg
50
605942
�
10137-38
PRICE
�������
���������������
�������
�
© Copyright 2004 Alltech Associates, Inc.
10
�
�
�
�
������
Contact your Alltech office or distributor for current or local prices.
SPE
Reversed-Phase SPE
C18 SPE Applications
Hypericins in St. John’s Wort
Hypericins from St. John’s Wort
�
Procedure Using Extract-Clean™ High Capacity C18, 1000mg:
9107
A. Sample Treatment
Pulverize 300mg St. John’s Wort powder into 5, 3mL
aliquots of 80/20 MeOH/H20. Combine and filter extracts to
produce 15mL filtrate. Dilute to 30mL with water.
1. Pseudohypericin
2. Hypericin
�
B. Conditioning
1. Rinse device with 5mL methanol.
2. Rinse device with 5mL deionized water.
C. Sample Application
Apply 2mL filtrate to the top of the SPE device and draw
through the C18 packed bed.
D. Wash*
Wash device with 3mL of deionized water.
E. Elution
Elute with 2mL methanol.
*Repeat load and wash steps consecutively until filtrate is consumed.
�
�
Column:
Mobile Phase:
Flow Rate:
Detector:
�
������
Alltima™ C18, 3µm 53 x 7mm Rocket™ HPLC Column
MeOH:0.2% H3PO4 (95:5) (Part No. 50605)
4.0mL/min
VIS at 585nm
Phenolic Acids in Echinacea
Phenolic Acids from Echinacea
�
Procedure Using Extract-Clean™ High Capacity C18, 1000mg:
A. Sample Treatment
Pulverize 300mg Echinacea powder into 5, 3mL aliquots
of methanol. Combine and filter extracts to produce 15mL
filtrate. Dilute to 30mL with water.
9110
1. Echinicoside
2. Chlorogenic Acid
3. Caffeic Acid
�
B. Conditioning
1. Rinse device with 5mL methanol.
2. Rinse device with 5mL deionized water.
C. Sample Application
Apply 2mL filtrate to the top of the SPE device and draw
through the C18 packed bed.
D. Wash*
Wash device with 2mL of deionized water.
E. Elution
Elute with 5mL of 50:50 methanol/water.
*Repeat load and wash steps consecutively until filtrate is consumed.
�
�
Column:
Mobile Phase:
Flow Rate:
Detector:
A. Conditioning
1. Rinse device with 10mL of hexane and 10mL of ethyl
acetate. Pass air through device to remove excess
solvent.
2. Rinse device with 5mL of methanol and 5mL of
deionized water.
D. Elution
Elute with 2mL of 70:30 hexane:ethyl acetate. Pass extract
through 2g-3g sodium sulfate to remove residual water.
© Copyright 2004 Alltech Associates, Inc.
Alltima C18, 3µm 53 x 7mm Rocket™ HPLC Column
ACN:10mM K2HPO4, pH 2.6 (20:80) (Part No. 50605)
3.5mL/min
UV at 330nm
Recovery (as % Standard)
1668
1. α−BHC (104%)
2. Lindane (109%)
�
�
3. β−BHC (106%)
7. Heptachlor Epoxide (107%)
4. Heptachlor (92%)
8. α-Endosulfan (105%)
�
5. δ−BHC (110%)
9. p,p’-DDE (79%)
�
� �
6. Aldrin (98%)
10. Dieldrin (102%)
�� ��
11. Endrin (104%)
��
12. p,p’-DDD (92%)
��
13. β-Endosulfan (103%)
��
��
14. p,p’-DDT
��
15. Endrin Aldehyde (102%)
�� ��
16. Endosulfan Sulfate (103%)
17. Methoxychlor (99%)
������� 18. Endrin Ketone (104%)
�
��
Procedure Using Extract-Clean™ Hi-Flow C18, 500mg:
C. Wash
Wash device with 10mL of deionized water then 10mL of 20:
80 methanol:deionized water. Remove excess by passing air
through the device for two minutes.
������
Chlorinated Pesticides, 1ppb
Chlorinated Pesticides from Water
B. Sample Application
Pass 100mL - 500mL (containing 1% methanol) of water
sample through the device at 20mL/minute.
�
™
�
�
Column:
Temp:
Carrier Gas:
Detector:
AT™-Pesticide Capillary GC Column,
20m x 0.53mm x 0.60µm (Part No. 16846)
210°C
Helium, 35cm/sec
ECD
Contact your Alltech office or distributor for current or local prices.
11
SPE
Reversed-Phase SPE
C18
• Choose for highly non-polar compounds
C18 Extended Volume Columns
C18 Specifications
PARTICLE
SIZE
SORBENT
Standard C18
50µm
High Capacity C18 50µm
High Flow C18
100µm
50µm
Large Pore C18
Oil & Grease C18
100µm
PORE CARBON
SIZE
LOAD
60Å
6%
60Å
17%
60Å
8%
150Å 14%
60Å
17%
CHOOSE FOR:
Optimized Recoveries
Stronger Retention
Viscous Samples
Macromolecules
Highly Viscous
Samples; Compatible
w/EPA 1664
C18 Extract-Clean™ and
Ultra-Clean™ Columns
6305
BED WEIGHT
100mg
200mg
500mg
C18 EXTENDED VOLUME COLUMNS
COLUMN SIZE
QTY.
PART NO.
20mL
30
405001
18mL
30
405150
18mL
30
405250
C18 Maxi-Clean™ Cartridges
4882
4886
C18 COLUMNS
BED WEIGHT
COLUMN SIZE
QTY.
PART NO.
C18 Extract-Clean™ Columns
50mg
1.5mL
100
204900
100mg
1.5mL
100
205000
200mg
4.0mL
50
205150
500mg
4.0mL
50
205250
500mg
8.0mL
30
205350
1000mg
8.0mL
30
205430
2000mg
8.0mL
30
205450
2000mg
15mL
30
205462
5000mg
25mL
20
225450
10,000mg
75mL
16
235410
High Capacity C18 Extract-Clean™ Columns
50mg
1.5mL
100
255050
100mg
1.5mL
100
255100
200mg
4.0mL
50
255200
500mg
4.0mL
50
255300
500mg
8.0mL
30
255350
1000mg
8.0mL
30
255430
2000mg
15mL
30
255440
5000mg
25mL
20
255450
10,000mg
75mL
16
255460
High Flow C18 Extract-Clean™ Columns
500mg
4.0mL
50
215250
1000mg
8.0mL
30
215430
Oil & Grease C18 Extract-Clean™ Columns
4000mg
25mL
20
202973
C18 Ultra-Clean™ Columns
200mg
4.0mL
50
505150
500mg
8.0mL
30
505355
© Copyright 2004 Alltech Associates, Inc.
12
PRICE
C18 MAXI-CLEAN CARTRIDGES
BED WEIGHT
QTY.
PART NO.
C18 Maxi-Clean™ Cartridges
300mg
25
20924
300mg
50
20926
300mg
100
20928
600mg
25
20932
600mg
50
20934
600mg
100
20936
900mg
25
20940
900mg
50
20942
900mg
100
20944
High Capacity C18 Maxi-Clean™ Cartridges
300mg
50
20945
Large Pore C18 Maxi-Clean™ Cartridges
300mg
100
22012
600mg
100
22017
900mg
100
220215
™
PRICE
PRICE
C18 96-Well Plates
• Standard 96-well format
• Square 2mL wells
4888
BED WEIGHT
25mg
50mg
100mg
C18 96-WELL PLATES
WELL VOLUME
QTY.
PART NO.
2.0mL
1
214996
2.0mL
1
204996
2.0mL
1
224996
PRICE
Contact your Alltech office or distributor for current or local prices.
SPE
Reversed-Phase SPE
C8
Phenyl
• Choose for compounds retained too strongly on C18
C8 Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
• Choose for highly aromatic compounds
Phenyl Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
Octyl (C8)
50µm irregular silica, 60Å
Yes
4.5%; 8.5% high capacity
Reversed-phase
C8 Extract-Clean™ and
Ultra-Clean™ Columns
Phenyl (PH)
50µm irregular silica, 60Å
Yes
3.8%
Reversed-phase
Phenyl Extract-Clean™ Columns
4886a
C8 COLUMNS
BED WEIGHT
COLUMN SIZE
QTY.
PART NO.
C8 Extract-Clean™ Columns
100mg
1.5mL
100
206000
200mg
4.0mL
50
206150
500mg
4.0mL
50
206250
500mg
8.0mL
30
206350
High Capacity C8 Extract-Clean™ Columns
100mg
1.5mL
100
206500
200mg
4.0mL
50
206550
500mg
4.0mL
50
206600
500mg
8.0mL
30
206650
C8 Ultra-Clean™ Columns
200mg
4.0mL
50
506151
500mg
4.0mL
50
506251
500mg
8.0mL
30
506351
4886a
PRICE
PART NO.
232062
232000
232150
232300
PRICE
Phenyl Maxi-Clean™ Cartridges
4882
PHENYL MAXI-CLEAN
QTY.
25
50
100
™
BED WEIGHT
300mg
300mg
300mg
C2
CARTRIDGES
PART NO.
22002
22003
22004
PRICE
Cyclohexyl
• Choose for compounds retained too strongly on C8
C2 Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
• Choose for cyclic or aromatic compounds
Cyclohexyl Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
Ethyl (C2)
50µm irregular silica, 60Å
Yes
5.5%
Reversed-phase
C2 Extract-Clean™ Columns
COLUMN SIZE
1.5mL
4.0mL
4.0mL
75mL
C2 COLUMNS
QTY.
100
50
50
16
© Copyright 2004 Alltech Associates, Inc.
Cyclohexyl (CH)
50µm irregular silica, 60Å
Yes
3.5%
Reversed-phase
Cyclohexyl Extract-Clean™
Columns
4886a
BED WEIGHT
100mg
200mg
500mg
10,000mg
PHENYL COLUMNS
COLUMN SIZE
QTY.
1.5mL
100
1.5mL
100
4.0mL
50
4.0mL
50
BED WEIGHT
50mg
100mg
200mg
500mg
PART NO.
207300
207450
207550
207616
4886a
PRICE
BED WEIGHT
200mg
500mg
1000mg
CYCLOHEXYL COLUMNS
COLUMN SIZE
QTY.
PART NO.
4.0mL
50
207150
4.0mL
50
207250
8.0mL
30
207331
PRICE
Contact your Alltech office or distributor for current or local prices.
13
SPE
Normal-Phase SPE
Cyanopropyl
Aminopropyl
• Choose for highly to moderately polar compounds
• Choose for polar compounds, or as a weak anion
exchanger
Aminopropyl Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
Aminopropyl (NH2)
50µm irregular silica, 60Å
No
5%
Weak anion exchange w/aqueous
matrix, normal-phase with non-polar
organic matrix
Cyanopropyl Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
Cyanopropyl (CN)
50µm irregular silica, 60Å
Yes
6%
Weak/moderate non-polar with
aqueous matrix, or polar with nonpolar organic matrix
Cyanopropyl Extract-Clean™
Columns
Aminopropyl Extract-Clean™
and Ultra-Clean™ Columns
4886a
4886a
AMINOPROPYL COLUMNS
PART NO.
QTY.
COLUMN SIZE
BED WEIGHT
Aminopropyl Extract-Clean™ Columns
100
1.5mL
100mg
211000
50
4.0mL
200mg
211025
50
4.0mL
500mg
211150
30
8.0mL
500mg
211256
30
8.0mL
1000mg
211153
Aminopropyl Ultra-Clean™ Columns
50
4.0mL
200mg
511025
50
4.0mL
500mg
511150
PRICE
CYANOPROPYL COLUMNS
PART NO.
QTY.
COLUMN SIZE
100
1.5mL
209300
50
4.0mL
209450
50
4.0mL
209550
30
8.0mL
209650
PRICE
Diol
• Choose for moderately polar compounds
Diol Specifications
Functional Group:
Base:
Endcapped:
Percent Carbon Loading:
Retention Mechanism:
Aminopropyl Extended Volume Columns
Diol (2OH)
50µm irregular silica, 60Å
No
4%
Normal-phase
Diol Extract-Clean™ Columns
6305
BED WEIGHT
500mg
BED WEIGHT
100mg
200mg
500mg
500mg
AMINOPROPYL EXTENDED VOLUME COLUMNS
PART NO.
QTY.
COLUMN SIZE
30
10.0mL
407250
PRICE
4886a
Aminopropyl Maxi-Clean Cartridges
™
COLUMN SIZE
1.5mL
4.0mL
4.0mL
BED WEIGHT
100mg
200mg
500mg
DIOL COLUMNS
QTY.
100
50
50
4882
AMINOPROPYL MAXI-CLEAN™ CARTRIDGES
PART NO.
QTY.
BED WEIGHT
50
300mg
210044
100
300mg
210046
100
900mg
210047
PRICE
PRICE
PART NO.
208000
208150
208250
Diol Maxi-Clean™ Cartridges
4882
DIOL MAXI-CLEAN CARTRIDGES
PART NO.
QTY.
50
210084
100
210086
™
BED WEIGHT
300mg
300mg
© Copyright 2004 Alltech Associates, Inc.
14
PRICE
Contact your Alltech office or distributor for current or local prices.
SPE
Normal-Phase SPE
Silica
• Choose for highly polar compounds
Silica Specifications
Functional Group:
Base:
Percent Carbon Loading:
Retention Mechanism:
Silica Maxi-Clean™ Cartridges
None
50µm irregular silica, 60Å
0%
Normal-phase
4882
Silica Extract-Clean and Ultra-Clean
Columns
™
™
4886
SILICA COLUMNS
QTY.
BED WEIGHT COLUMN SIZE EPA METHOD
Silica Extract-Clean™ Columns
100
—
1.5mL
50mg
100
—
1.5mL
100mg
50
—
4.0mL
200mg
50
—
4.0mL
500mg
30
3630C
8.0mL
500mg
30
3630C
8.0mL
1000mg
30
—
8.0mL
2000mg
30
3630C
15mL
2000mg
20
—
25mL
5000mg
20
—
25mL
10,000mg
16
—
75mL
10,000mg
16
—
75mL
20,000mg
16
—
75mL
30,000mg
Silica Ultra-Clean™ Columns
50
—
4.0mL
200mg
30
3630C
8.0mL
500mg
20
—
25.0mL
5000mg
PART NO.
PRICE
209062
209000
209150
209250
209200
209100
209202
209362
22935
239300
239310
239322
239330
BED WEIGHT
300mg
300mg
300mg
600mg
600mg
600mg
900mg
900mg
900mg
SILICA MAXI-CLEAN™ CARTRIDGES
PART NO.
QTY.
25
20972
50
20974
100
20976
25
20980
50
20982
100
20984
25
20988
50
20990
100
20992
PRICE
Silica 96-Well Plates
4886
BED WEIGHT
25mg
50mg
100mg
509150
509225
509010
SILICA 96-WELL PLATES
PART NO.
QTY.
WELL VOLUME
1
2.0mL
219096
1
2.0mL
209096
1
2.0mL
229096
PRICE
Silica Extended Volume Columns
Could your sample results
be improved by a more inert
SPE column?
6305
BED WEIGHT
100mg
200mg
500mg
SILICA EXTENDED VOLUME COLUMNS
PART NO.
QTY.
COLUMN SIZE EPA METHOD
30
—
10.0mL
409001
30
—
10.0mL
409150
30
3630C
10.0mL
409250
© Copyright 2004 Alltech Associates, Inc.
PRICE
Choose Ultra-Clean™ columns for
critical applications that can benefit
from an inert glass-like column.
Contact your Alltech office or distributor for current or local prices.
15
SPE
Normal-Phase SPE
Florisil® and Florisil®-PR
Alumina
• Choose for pesticides and metal organics. Florisil®-PR
is tested specifically for chlorinated pesticides
Florisil® Specifications
Sorbent:
Base:
Particle Size:
Retention Mechanism:
Florisil®
Magnesium Silicate, 60Å porosity
75-150µm
Normal-phase
Florisil® Extract-Clean™ and
Ultra-Clean™ Columns
• Alumina-Acidic—Wash with acidic solution to
increase capacity for neutral or anionic compounds
• Alumina-Basic—Wash with basic solution to increase
capacity for cationic compounds
• Alumina-Neutral—Neutral surface interacts with
highly aromatic compounds and neutral hydroxyls
Alumina Specifications
Sorbent:
Base:
pH
Particle Size:
Retention Mechanism:
4886a
FLORISIL AND FLORISIL -PR COLUMNS
BED WEIGHT COLUMN SIZE EPA METHOD QTY.
PART NO.
Florisil® Extract-Clean™ Columns
100mg
1.5mL
—
100
204500
500mg
4.0mL
3620B
50
204650
1000mg
8.0mL
3620B
30
207930
2000mg
15mL
3620B
30
207962
5000mg
25mL
—
20
227950
10,000mg
75mL
—
16
237910
Florisil® Ultra-Clean™ Columns
500mg
4.0mL
3620B
50
507851
1000mg
8.0mL
3620B
30
507930
Florisil®-PR Extract-Clean™ Columns
500mg
4.0mL
—
50
250015
1000mg
8.0mL
—
30
250020
10,000mg
75mL
—
16
250910
Florisil®-PR Ultra-Clean™ Columns
500mg
4.0mL
—
50
504651
1000mg
8.0mL
—
30
507900
®
Alumina
Aluminum Oxide, 100Å porosity
4.5 (Acidic), 10.0 (Basic), 7.5 (Neutral)
130µm
Lewis acid/base, polar, and ion
exchange
®
PRICE
Alumina Extract-Clean™
Columns
4886a
ALUMINA TYPE
Acidic
Acidic
Basic
Basic
Neutral
Neutral
Neutral
Florisil® Extended Volume
Columns
ALUMINA EXTRACT-CLEAN™ COLUMNS
BED WEIGHT COLUMN SIZE QTY. PART NO.
100mg
1.5mL
100 228200
500mg
4.0mL
50 228350
100mg
1.5mL
100 228000
500mg
4.0mL
50 228150
100mg
1.5mL
100 228400
500mg
4.0mL
50 228550
2000mg
15mL
30
22856
Alumina Extended Volume
Columns
6305
BED WEIGHT
100mg
500mg
PRICE
6305
FLORISIL® EXTENDED VOLUME COLUMNS
COLUMN SIZE EPA METHOD QTY. PART NO.
10.0mL
—
30
407600
10.0mL
3620B
30
407850
PRICE
Florisil® Maxi-Clean™ Cartridges
ALUMINA EXTENDED VOLUME COLUMNS
ALUMINA TYPE BED WEIGHT COLUMN SIZE QTY. PART NO.
Acidic
500mg
10.0mL
30 410450
Basic
500mg
10.0mL
30 410250
Neutral
500mg
10.0mL
30 410850
Neutral 11%
500mg
10.0mL
30 410730
deactivated*
PRICE
* Larger package sizes are available.
Alumina Maxi-Clean™ Cartridges
4882
FLORISIL® MAXI-CLEAN™ CARTRIDGES
BED WEIGHT
QTY.
PART NO.
Florisil® Maxi-Clean™ Cartridges
300mg
50
210054
300mg
100
210056
900mg
50
210057
900mg
100
210061
Florisil®-PR Maxi-Clean™ Cartridges
300mg
50
210074
300mg
100
210076
900mg
50
210079
900mg
100
210075
© Copyright 2004 Alltech Associates, Inc.
16
PRICE
4882
ALUMINA TYPE
Acidic
Acidic
Basic
Basic
Neutral
Neutral
ALUMINA MAXI-CLEAN™ CARTRIDGES
BED WEIGHT
QTY.
PART NO.
300mg
25
210094
1800mg
25
210097
300mg
25
210093
1800mg
25
210096
300mg
25
210095
1800mg
25
210098
PRICE
Contact your Alltech office or distributor for current or local prices.
SPE
Specialty SPE
DVB
Carbograph
DVB Extract-Clean™ Columns
Carbograph Extract-Clean™ and Ultra-Clean™
Columns
• Greater sample capacity than C18
• 100% divinylbenzene reduces swelling
• 40µm average particle size
• Graphitized carbon retains polar organics in aqueous
matrices
• Acid, base-neutral extraction of pesticides and
herbicides
• 100m2/g surface area
4884
Extract-Clean™ DVB is useful in a broad range of
applications, such as extracting phenols and acidic
pesticides from environmental samples. It is also useful
for polar drug metabolites and nucleic acid preparations.
The presence of free vinyl groups also makes the resin
useful as a solid-phase synthesis support.
4891
DVB EXTRACT-CLEAN COLUMNS
COLUMN SIZE
QTY.
PART NO.
1.5mL
100
220025
1.5mL
100
220050
1.5mL
100
220100
4.0mL
50
220500
™
BED WEIGHT
25mg
50mg
100mg
500mg
PRICE
Drying Tubes
Maxi-Clean™ and Extract-Clean™ Drying Tubes
CARBOGRAPH COLUMNS
BED WEIGHT
COLUMN SIZE
QTY.
PART NO.
Carbograph Extract-Clean™ Columns
150mg
4.0mL
50
210142
300mg
8.0mL
30
210101
500mg
8.0mL
30
210150
1000mg
15mL
20
210121
Carbograph Ultra-Clean™ Columns
150mg
4.0mL
50
510142
300mg
8.0mL
30
510101
PRICE
These SPE tubes are packed with anhydrous sodium
sulfate to remove residual water from SPE extracts. They
are suitable for pesticide analysis.
Phase Separator Columns
4884
DEVICE
Maxi-Clean™ Cartridge, 2g
Extract-Clean™ Column, 3g
DRYING TUBES
QTY.
100
100
4895
PART NO.
219001
219002
PRICE
Use Phase Separator Columns for fast and simple
separation of organic and aqueous mixed samples.
The column contains a 20µm polyethylene frit and
a hydrophobic silicone membrane that allows the
hydrophobic phase to pass through, while the aqueous
phase is retained in the upper chamber.
Sephadex™ SPE Columns
Sephadex™ columns are commonly used for desalting
procedures and for size-exclusion.
- G-25 for 1000-5000 molecular weight range.
- G-50 for 1500-30,000 molecular weight range.
DESCRIPTION
G-25
G-50
SEPHADEX™ SPE COLUMNS
BED VOLUME COLUMN SIZE QTY. PART NO.
1.0mL
4.0mL
50
201200
1.0mL
4.0mL
50
201300
© Copyright 2004 Alltech Associates, Inc.
PRICE
4892
COLUMN SIZE
4.0mL
8.0mL
25mL
PHASE SEPARATOR COLUMNS
QTY.
PART NO.
100
205289
100
205389
100
205589
PRICE
Contact your Alltech office or distributor for current or local prices.
17
SPE
Ion-Exchange SPE
SCX
SAX
SAX Specifications
Functional Group:
Base:
Capacity:
Retention Mechanism:
Tetramethyl Ammonium
Styrene-divinylbenzene
0.15meq/100mg
Strong anion exchanger in acetate form;
retains (-) charged compounds.
SAX Extract-Clean™ Columns
SCX Specifications
Functional Group:
Base:
Capacity:
Retention Mechanism:
Benzenesulfonic Acid
Styrene-divinylbenzene
0.24meq/100mg
Strong cation exchanger in hydrogen
form; retains (+) charged compounds.
SCX Extract-Clean™ Columns
4889
4889
SAX COLUMNS
QTY.
COLUMN SIZE
BED WEIGHT
SAX Extract-Clean™ Columns
100
1.5mL
100mg
50
4.0mL
200mg
50
4.0mL
500mg
30
8.0mL
1000mg
SAX Ultra-Clean™ Columns
50
4.0mL
200mg
50
4.0mL
500mg
PART NO.
PRICE
209600
209625
209750
209850
509826
509951
SCX COLUMNS
QTY.
COLUMN SIZE
BED WEIGHT
SCX Extract-Clean™ Columns
100
1.5mL
100mg
50
4.0mL
200mg
50
4.0mL
500mg
30
8.0mL
1000mg
SCX Ultra-Clean™ Columns
50
4.0mL
200mg
50
4.0mL
500mg
209800
209825
209950
209930
509626
509751
6305a
6305a
SAX EXTENDED VOLUME COLUMNS
PART NO.
QTY.
COLUMN SIZE
30
10.0mL
409750
PRICE
SAX Maxi-Clean™ Cartridges
SCX EXTENDED VOLUME COLUMNS
PART NO.
QTY.
COLUMN SIZE
30
10.0mL
409950
BED WEIGHT
500mg
BED WEIGHT
600mg
600mg
600mg
PRICE
SAX 96-Well Plates
4897
BED WEIGHT
600mg
600mg
600mg
SCX MAXI-CLEAN™ CARTRIDGES
PART NO.
QTY.
25
21901
50
21902
100
21903
SAX 96-WELL PLATES
PART NO.
QTY.
WELL VOLUME
1
2.0mL
219696
1
2.0mL
209696
1
2.0mL
229696
© Copyright 2004 Alltech Associates, Inc.
18
PRICE
SCX 96-Well Plates
4888
BED WEIGHT
25mg
50mg
100mg
PRICE
SCX Maxi-Clean™ Cartridges
4897
SAX MAXI-CLEAN™ CARTRIDGES
PART NO.
QTY.
25
21906
50
21907
100
21908
PRICE
SCX Extended Volume Columns
SAX Extended Volume Columns
BED WEIGHT
500mg
PART NO.
PRICE
4888
BED WEIGHT
25mg
50mg
100mg
SCX 96-WELL PLATES
PART NO.
QTY.
WELL VOLUME
1
2.0mL
219896
1
2.0mL
209896
1
2.0mL
229896
PRICE
Contact your Alltech office or distributor for current or local prices.
SPE
IC SPE
Maxi-Clean™ IC
• Eliminate matrix interferences before ion analysis
• Seven chemistries solve a variety of specific problems
The most difficult part of many IC applications is
eliminating interfering components from the sample
matrix. These interferences may co-elute or mask peaks
of interest, overload the column, or shorten the column
life by binding irreversibly to the column packing.
Alltech’s unique ion-exchange SPE cartridges eliminate
many of these interferences1, 2.
Each metal-free polypropylene cartridge contains
0.5mL or 1.5mL of purified polystyrene resin, contained
by 20µm polyethylene frits. The resin is functionalized
to retain specific types of components from the sample.
4893
R. Saari-Nordhaus, J.M. Anderson, Jr., and I.K. Henderson, Am. Lab.
August (1990) 19.
2
I.K. Henderson, R. Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr.
546 (1991) 61.
1
IC SPE Specifications
RESIN
Polystyrene
IC-OH
Strong anion exchanger
in hydroxide form
IC-H
Strong cation exchanger
in H+ form
Cations
0.8 / 2.0
IC-Ag
Strong cation exchanger
in Ag+ form
Strong cation exchanger
in Ba+2 form
Strong cation exchanger
in Na+ form
Chloride Iodide
Bromide
Sulfate
0.8 / 2.0
Cations
0.8 / 2.0
Styrene-divinylbenzene
containing chelating
group (iminodiacetate in
Na+ form)
Polystyrene/strong anion
exchanger in hydroxide
form
Polystyrene/strong
cation exchanger in
hydrogen form
Polyvalent metal
ions
0.2 / 0.5
Hydrophobic
components and
anions
Hydrophobic
components and
cations
Variable-0.6
Simultaneously removes hydrophobic components and
anions and increases the pH of acidic samples.
Variable-0.8
Simultaneously removes hydrophobic components and
cations and reduces the pH of basic samples.
IC-Ba
IC-Na
IC-Chelate
Mixed-Mode RP-OH
Mixed-Mode RP-H
RETAINS
Hydrophobic
components
Anions
CAPACITY (MEQ)
0.5ML/1.5ML
Variable
CARTRIDGE
IC-RP
Looking for Vacuum
Manifolds?
See pages 24-25 for vacuum
manifolds to process samples.
0.6 / 1.5
0.8 / 2.0
APPLICATIONS
Removes surfactants, organic acids, and other organic
substances. Inorganic ions pass through.
Exchanges anions for hydroxide. May be used to remove
or concentrate anions from sample and to increase pH
of acidic samples. Removes cations that form insoluble
hydroxide salts.
Exchanges cations for H+. May be used to remove or
concentrate cations from sample and to reduce pH of
basic samples.
Removes excess halides through formation of Ag-halide
salts.
Removes excess sulfate through formation of BaSO4.
Exchanges cations for Na+. May be used to remove or
retain cations from sample without changing the pH of
the sample.
Exchanges transition metals and divalent cations for
Na+. May be used to remove or retain divalent cations
and transition metals from sample.
For our full selection of IC columns,
consumables, and instruments see
pages 304-327.
4870
© Copyright 2004 Alltech Associates, Inc.
Contact your Alltech office or distributor for current or local prices.
19
SPE
IC SPE
IC SPE Extract-Clean™
Columns
IC SPE Maxi-Clean™
Cartridges
• Process multiple samples with vacuum manifold
• Two bed volumes available
• For labs with higher sample throughput requirements
• Use with any luer
tip syringe
• Two bed volumes
available
4893
IC EXTRACT-CLEAN™ COLUMNS
RESIN
COLUMN SIZE
QTY.
PART NO.
0.5mL Bed Volume
IC-RP
4.0mL
50
40260
IC-OH
4.0mL
50
40262
IC-H
4.0mL
50
40264
IC-Ag
4.0mL
50
105050
IC-Ba
4.0mL
50
40268
IC-Na
4.0mL
50
40270
IC-Chelate
4.0mL
50
40250
1.5mL Bed Volume
IC-RP
4.0mL
30
140252
IC-OH
4.0mL
30
140254
IC-H
4.0mL
30
140256
IC-Ag
4.0mL
30
140258
IC-Ba
4.0mL
30
140261
IC-Na
4.0mL
30
140263
IC-Chelate
4.0mL
30
140265
PRICE
Organic and hydrophobic contaminants may not
interfere with your chromatography, but they can reduce
the life of your expensive analytical columns. The photo
below shows the contaminants removed from betadine,
while the chromatogram shows that using IC-RP to
remove these contaminants does not interfere with your
separation.
IC MAXI-CLEAN™ CARTRIDGES
RESIN
QTY.
PART NO.
0.5mL Bed Volume
IC-RP
50
30260
IC-OH
50
30262
IC-H
50
30264
IC-Ag
50
30266
IC-Ba
50
30268
IC-Na
50
30270
IC-Chelate
50
30250
Mixed-Mode RP-OH
25
30259
Mixed-Mode RP-H
25
30267
1.5mL Bed Volume
IC-RP
25
30252
IC-OH
25
30254
IC-H
25
30256
IC-Ag
25
30258
IC-Ba
25
30261
IC-Na
25
30263
IC-Chelate
25
30265
PRICE
IC-RP Protects Analytical Columns by Removing
Hydrophobic Contaminants
Iodide in Betadine Sample After Treatment with IC-RP
5
12
0
3
1.
2.
3.
4.
5.
Fluoride
Chloride
Phosphate
Sulfate
Iodide
13003
4
4
8
12 Min.
Column:
IC-RP bed
before
extraction
IC-RP bed
after
extraction
Novosep™ Anion, 7µm, 100 x 4.6mm
HPLC Column (Part No. 51406)
Mobile Phase: 5mM Sodium Carbonate
Flow Rate:
1.0mL/min
Detector:
Suppressed Conductivity
6381
© Copyright 2004 Alltech Associates, Inc.
20
Contact your Alltech office or distributor for current or local prices.
SPE
IC SPE
IC SPE Applications
IC-Ag Removes Chloride
IC-Ba Removes Sulfate
Nitrite in Brine
Anions in Battery Acid
10059
10060
Unextracted
10064
Extracted
1. Fluoride
2. Nitrate
3. Sulfate
10065
Unextracted
Extracted
1.
2.
3.
4.
5.
� �
5
Fluoride
Chloride
Nitrite
Nitrate
Sulfate
2
�
0
4
8
12
16
20 min.
�
5
1
�
�
��
��
�������
0
4
8
12
16
20 Min.
0
34
4
8
16
20 Min.
Column:
Column:
Novosep™ Anion, 7µm, 150 x 4.6mm
IC Column (Part No. 51407)
Mobile Phase: 1.7mN NaHCO3/1.8mM Na2CO3
Flow Rate:
1.5mL/min
Detector:
Conductivity (Suppressed)
Novosep™ A1, 7µm, 150 x 4.6mm
IC Column (Part No. 51407)
Mobile Phase: 1.7mM NaHCO3/1.8mM Na2CO3
Flow Rate:
1.2mL/min
Detector:
Conductivity (Suppressed)
IC-H Neutralizes Hydroxide
IC-OH Neutralizes Acid
Trace Anions in Caustic
Nitric Acid
10069
10070
Unextracted
12
Extracted
10062
10063
Unextracted
1. Fluoride
2. Chloride
3. Nitrate
Extracted
1. Sodium
2. Magnesium
3. Calcium
�
�
� �
�
�
�
�
�
�������
Column:
�
�
�
�
Novosep™ A1, 7µm, 150 x 4.6mm
IC Column (Part No. 51407)
Mobile Phase: 1.7mM NaHCO3 / 1.8mM Na2CO3
Flow Rate:
1.2mL/min
Detector:
Conductivity (Suppressed)
© Copyright 2004 Alltech Associates, Inc.
��
�
�������
� � � � � �� �� �������
� � � � � �� �� �������
Column:
Universal Cation, 7µm, 100 x 4.6mm
IC Column (Part No. 27106)
Mobile Phase: 3mM Methanesulfonic Acid
Flow Rate:
1.0mL/min
Detector:
Conductivity (Non-Suppressed)
Contact your Alltech office or distributor for current or local prices.
21
SPE
Custom SPE and Accessories
Custom Packed SPE Products
While we list the most popular sorbent and column
combinations in our standard product line, we realize
that there are many new and exciting applications that
may require something special.
SPE Custom Quote Request to Alltech
Date:
FAX To: 814-234-3594
Or Email: spequote@alltechemail.com
Alltech will custom pack any of our columns or
cartridges to your specifications. We will consider all
requests, large or small.
FAX or email your custom request on your letterhead
for a price quotation. Be sure to include a contact name
and phone number.
Please specify:
1. Packing Material
2. Quantity Per Device
3. Device Size and Style
Custom orders are not returnable. A minimum order
may be required.
Contact Name:
Contact Phone Number:
Alltech Part No.: C-27100 - Custom Order
Packing Material:
Amount of Packing Material:
Device Size:
Device Style:
Extract-Clean™ Column
(choose one)
Maxi-Clean™ Cartridge
Ultra-Clean™ Treated Column
Extended Volume Column
Total Pieces Required:
Special Instructions:
Bulk SPE Packings
Filter Columns
These bulk packings are the same
high-quality material we manufacture for
our Extract-Clean™ and Maxi-Clean™ SPE
products.
Filter columns are Extract-Clean™ reservoirs with
two frits at the outlet end. They remove particulate
matter down to 20µm from samples. Syringe adapters
will connect filter columns to the tops of Extract-Clean™
columns.
4900
BULK SPE PACKINGS
DESCRIPTION
QTY.
Reversed-Phase Adsorbents
Prevail C18 (11%)
100g
C18 (6%)
100g
C18 (17%)
100g
Large Pore C18 (14%)
100g
C8
100g
C2
100g
Phenyl
100g
Normal-Phase Adsorbents
Silica
100g
Aminopropyl
100g
Diol
100g
Florisil®
227g
Florisil® PR
227g
Alumina-N
100g
Ion Exchange
SCX (Cation Exchange)
100g
SAX (Anion Exchange)
100g
25g
Carbograph 1 SPE 120/400 Mesh
© Copyright 2004 Alltech Associates, Inc.
22
PART NO.
211505
211502
211503
211542
211504
211506
211522
PRICE
EXTRACT-CLEAN™ FILTER COLUMNS
DESCRIPTION
QTY.
PART NO.
1.5mL Filter Columns
100
211101
4.0mL Filter Columns
50
211104
8.0mL Filter Columns
50
211108
75.0mL Filter Columns
50
210775
PRICE
211512
211516
211518
5618
5615
211541
211520
211510
1769
See pages 2-5 for help choosing a sorbent
and device.
Contact your Alltech office or distributor for current or local prices.
SPE
SPE Accessories
Reservoirs and Frits
Adapters & Caps
• Inlet caps fit SPE devices as indicated.
• Outlet caps fit any column or cartridge with a male
luer tip.
Select empty reservoirs and loose frits to pack your own
custom SPE columns.
BULK RESERVOIRS AND FRITS
DESCRIPTION
QTY.
PART NO.
Extract-Clean™ Empty Reservoirs, Polypropylene
1.5mL
100
210001
1.5mL
500
210011
4.0mL
100
210104
8.0mL
100
210208
15.0mL
100
210315
25.0mL
100
210425
75.0mL
50
210575
Extended Volume Empty Reservoirs, Polypropylene
Up to 10.0mL Sample Volume
100
430050
Up to 10.0mL Sample Volume
1000
420011
Ultra-Clean™ Empty Reservoirs, Treated Polypropylene
4.0mL
100
70216
8.0mL
100
70218
8.0mL
1000
70069
20µm Polyethylene Frits for Extract-Clean™
For 1.5mL Reservoir
100
211401
For 4.0mL or EV Reservoir
100
211404
For 8.0mL Reservoir
100
211408
For 15.0mL Reservoir
100
211412
For 25.0mL Reservoir
100
211416
For 75.0mL Reservoir
100
211775
10µm PTFE Frits for Ultra-Clean™
For 4.0mL Reservoir
100
211494
For 8.0mL Reservoir
100
211498
6288
PRICE
5952
CAPS
DESCRIPTION
Inlet Caps for Maxi-Clean™ Cartridges
Inlet Caps for 1.5mL
Inlet Caps for 4.0mL
Inlet Caps for 8.0mL
Inlet Caps for 15.0mL or EV
Inlet Caps for 25.0mL
Inlet Caps for 75.0mL
Outlet Caps for Male Luers
• Square, 2mL wells
• All-polypropylene construction
• Use to pack your own custom
SPE plates
SYRINGE ADAPTERS
DESCRIPTION
QTY.
Syringe Adapters for 1.5mL, 4.0mL,
15
8.0mL, 15mL, and EV Columns
PART NO.
210705
Syringe Adapters for 25mL Columns
Syringe Adapters for 75mL Columns
210707
210709
4888
PRICE
5
5
PRICE
Looking for One-Step
Mobile Phase Clean-Up?
Standard 96-well plate
footprint (8x12 wells)
PART NO.
210780
PART NO.
211015
220000
220301
220600
221200
221006
227503
220710
Syringe Adapters fit the tops of SPE columns allowing
attachment of any male luer device. This lets you
process an Extract-Clean™ column with a syringe or add
an empty reservoir to increase sample volume capacity.
Empty 96-Well Plates
96-WELL PLATES
BED WEIGHT
QTY.
Empty plate with bottom frit
1
installed, top frit supplied
QTY.
50
50
50
50
50
50
50
50
Try SuperClean™ SPE columns on
page 39.
PRICE
5953
© Copyright 2004 Alltech Associates, Inc.
Contact your Alltech office or distributor for current or local prices.
23
SPE
SPE Accessories
Alltech Vacuum
Manifolds
I
• 12-, 16-, and 24-port
manifolds
• Glass chamber for visual
monitoring
• Accepts standard male luer
devices
Vacuum manifolds process
multiple samples simultaneously,
saving time and effort. Manifold
systems come complete with
the components listed below.
Stainless steel or PTFE needles are
available separately.
C
F
H
E
A
J
B
E
I
D
G
6278
12-Port Manifold
Vacuum Manifold Components
A.
B.
C.
D.
E.
F.
G.
H.
I.
J.
• For up to 12 samples
• Also includes one waste
container
4869
12-PORT MANIFOLD & ACCESSORIES
DESCRIPTION
QTY.
PART NO.
ea
12-Port Vacuum Manifold**
210351
Replacement Parts
Lid, Gaskets, and 12 Stopcocks
ea
212001
Glass Chamber
ea
213212
Vacuum Gauge, Valve, & Glass Chamber
ea
212304
Collection Rack, 12-Port Size*
ea
212518
Gaskets, 12-Port Size
2
212112
One-Way Stopcocks
12
213112
Waste Container
2
210033
PRICE
Glass Chamber
Vacuum Valve and Gauge
Polypropylene Lid
Stopcock Valves
Collection Rack Plates
Support Posts for Collection Racks
Retaining Clips for Collection Racks
Lid Legs
Manifold Inlet Caps
Polypropylene Needles
24-Port Manifold
• Process up to 24 samples
*12-Port Collection Rack includes 3 support posts, bottom plate, 13- and 16mm
plates, autosampler vial plate, volumetric plate, and 12 retaining clips.
** Includes waste container.
4871
16-Port Manifold
24-PORT MANIFOLD & ACCESSORIES
DESCRIPTION
QTY.
PART NO.
ea
24-Port Vacuum Manifold
210224
Replacement Parts
Lid, Gaskets, and 24 Stopcocks
ea
211224
Glass Chamber
ea
210124
Vacuum Gauge, Valve, & Glass Chamber
ea
210324
Collection Rack, 24-Port Size†
ea
210424
Gaskets, 16- and 24-Port Size
2
210724
One-Way Stopcocks
24
211524
• Process up to 16 samples
4870
16-PORT MANIFOLD & ACCESSORIES
DESCRIPTION
QTY.
PART NO.
ea
16-Port Vacuum Manifold
210016
Replacement Parts
Lid, Gaskets, and 16 Stopcocks
ea
210216
Glass Chamber
ea
210116
Vacuum Gauge, Valve, & Glass Chamber
ea
210316
Collection Rack, 16-Port Size†
ea
210416
Gaskets, 16- and 24-Port Size
2
210724
One-Way Stopcocks
16
211521
© Copyright 2004 Alltech Associates, Inc.
24
PRICE
PRICE
16- and 24-Port Collection Racks include 3 support posts, bottom plate, dimple
plate, 13- and 16mm plates, and 12 retaining clips.
†
Contact your Alltech office or distributor for current or local prices.
SPE
SPE Accessories
Manifold Accessories
Replacement Parts for All Size Manifolds
Drying Attachments
Manifolds come complete with all necessary gauges
and accessories. Individual replacement parts may be
sold separately.
Use these special drying lids to direct a gas flow into
collection tubes to dry eluants. Barb is 1/4".
5951
REPLACEMENT PARTS FOR ALL SIZE MANIFOLDS
DESCRIPTION
QTY.
PART NO.
Vacuum Gauge and Valve
ea
212203
Retaining Clips For Collections Racks
12
212912
Female Luer Inlet, Polypropylene
2
212002
Female Luer Inlet, Polypropylene
24/pk 212302
Male Luer Outlet, Polypropylene
2
212120
Male Luer Outlet, Polypropylene
24/pk 212320
Caps for Lid Inlets
50
211234
Lid Legs, Black Delrin
4
410510
Collection Rack Posts, Polypropylene
3
410410
PRICE
4878
DRYING ATTACHMENTS
DESCRIPTION
QTY.
12-Port Drying Attachment
1
16-Port Drying Attachment
1
24-Port Drying Attachment
1
PART NO.
212100
212117
212124
PRICE
Manifold Needles
Tygon® Tubing
Tygon® vacuum tubing is heavy walled and flexible.
It withstands 29.9" Hg at 75°F (24°C). It is nontoxic,
nonoxidizing, and transparent.
PTFE needles are disposable, fit many different
manifold types, and eliminate cross contamination by
extending into the collection tube. They also provide
a complete PTFE fluid path for samples to virtually
eliminate extractables. Available plain or with a valve.
Stainless Steel and Polypropylene needles are also
available.
Stainless Steel
PTFE
Polypropylene
Valved PTFE
4874
3849
TYGON® VACUUM TUBING
DESCRIPTION
QTY.
PART NO.
1/8" i.d., 3/8" o.d.
50'
6465
3/16" i.d., 9/16" o.d.
10'
6467
1/4" i.d., 3/4" o.d.
10'
6469
3/8" i.d., 7/8" o.d.*
10'
6472
*Use with Vacuum Gauge and Valve displayed above.
© Copyright 2004 Alltech Associates, Inc.
PRICE
DESCRIPTION
PTFE Needles
PTFE Needles
Valved PTFE Needles
Valved PTFE Needles
Stainless Steel Needles
Stainless Steel Needles
Stainless Steel Needles
Polypropylene Needles
Polypropylene Needles
Polypropylene Needles
MANIFOLD NEEDLES
QTY.
100
500
25
50
12
16
24
12
16
24
PART NO.
412410
412450
411525
411550
212400
210816
210824
212412
210916
210924
PRICE
Contact your Alltech office or distributor for current or local prices.
25
SPE
SPE Accessories
Benchtop Vacuum Station Vacuum/Pressure Station
• Pulls vacuum to 28.5" Hg
• Portable and low maintenance
• Continuous LCD display of vacuum level
• Pulls vacuum to 20" Hg, pressure to 18 psig
• Extended life diaphragm
• No vacuum oil required
• Eliminates need for water aspirators
4178
5691
Alltech’s Benchtop Vacuum Station is a versatile,
powerful, user-friendly answer to most vacuum
applications. Three modes of operation offer flexibility
for different applications. “Continuous Vacuum Mode”
pulls a maximum vacuum with a 1-120 minute timer.
“Continuous Vent Mode” clears the pump heads of vapors.
“Set Point Mode” allows you to select the vacuum level,
and it maintains that vacuum level with an absolute
vacuum sensor, making runs reproducible regardless of
altitude or barometric conditions. It is the perfect pump
for filtration and solid phase extraction tasks.
This Vacuum/Pressure station is an economical choice
for the busy lab. By simply switching ports, it can be
changed from a vacuum source to a pressure station.
Benchtop Vacuum Station Specifications
725mm Hg (28.5")
Maximum Pull:
6L/min
Flow Rate:
1-120 minutes
Timer:
8.7" W x 9.5" D x 4.5" H
Dimensions:
(22cm W x 24.2cm D x 11.5cm H)
Vacuum/Pressure Station Specifications
20" Hg
Vacuum Pull:
18psig
Pressure:
0.5cu. ft/min
Free Air Capacity:
8.75" H x 8" W x 7.5" L
Dimensions:
(22.23cm H x 20.32cm W x 19.05cm L)
DESCRIPTION
115V
230V
BENCHTOP VACUUM STATION
PART NO.
6417
6419
PRICE
The unit combines a single-head pump, vacuum/
pressure gauge, and stainless steel regulating valves.
DESCRIPTION
115V
230V
VACUUM/PRESSURE STATION
PART NO.
1670
1595
PRICE
VACUUM/PRESSURE REPLACEMENT PARTS/ACCESSORIES
DESCRIPTION
PART NO.
PRICE
Replacement Parts
Vacuum/Pressure Gauge Connector Kit:
1590
Includes Vacuum Regulation Valve Assembly,
Pressure Regulation Valve Assembly, and a
Manifold Chamber
Service Kit:
Includes Plastic Top with Retainers, Valves,
Nitrile Diaphragm and Eccentric Assembly
Accessories
Vacuum/Pressure Gauge
1592
1597
4384
© Copyright 2004 Alltech Associates, Inc.
26
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Filtration Introduction
Product Selection
There are several basic steps to choosing the best
filtration product for your needs. This guide will help
you get started.
1. Choose a Filter Membrane
Refer to the Membrane Selection Guide and the Chemical
Compatibility Chart on the next pages to select the
appropriate membrane for your application.
Consider
a.) sample particulate size
b.) aqueous or organic nature of the sample, and
c.) the levels of solvent resistance and protein
retention you require.
Filter membranes remove particulates from a sample or
mobile phase. 0.45µm pores are commonly used to remove
sample or mobile phase particulates prior to HPLC analysis.
0.2µm pores remove bacteria from biological samples and
can be used for sterilization.
Filter Capacities
Filter Specifications
DESCRIPTION
Syringe Filter Capacities
4mm Syringe Filter
13mm Syringe Filter
17mm Syringe Filter
25mm Syringe Filter
30mm Syringe Filter
Centrifuge Filter Tube Capacities
Ultra-Spin™ Tubes
Micro-Spin® Tubes
Maxi-Spin™ Tubes
Pre-Cut Membrane Capacities
13mm Pre-Cut Membranes
25mm Pre-Cut Membranes
47mm Pre-Cut Membranes
90mm Pre-Cut Membranes
SAMPLE VOLUME
≤1mL
≤5mL
≤50mL
≤50mL
≤100mL
20µL to 500µL
20µL to 500µL
1mL to 25mL
up to 20mL
up to 100mL
multi-liter
multi-liter
2. Choose a Membrane Diameter
Larger sample volumes require larger membrane diameters.
High particulate levels will reduce the amount of sample
filtered before a membrane becomes plugged, so use a
larger diameter membrane or a glass pre-filter to increase
filtrate volume with particulate laden samples.
3. Choose a Device
Consider the number of samples, the sample volume, and
the filtering mechanism you prefer to use.
Syringe Filters: Filtration is achieved by pushing the
sample through the membrane with a syringe or other
luer-connection device. Syringe filters allow you to
control the rate of flow, which can be critical with delicate
samples. Syringe filters also allow you to filter into nearly
any tube, vial, or column that represents the next step in
your analysis. Syringe filters with glass pre-filters are used
in the same manner as regular syringe filters. They are
especially useful for particulate laden samples.
Centrifuge Filter Tubes: Filtration is achieved by pulling
the sample through the membrane with centrifugal force.
This allows you to simultaneously filter as many samples
as your centrifuge can hold, and captures your filtrate in a
clean microtube.
Pre-Cut Membranes: Multiple diameters offer a wide range
of sample capacities, up to multiple liters. You need
a filtration apparatus, such as a filter funnel or other
vacuum-driven filtration device.
What is the advantage of a glass
pre-filter?
Pre-filters rest on top of the
filter membrane and help trap large
particulates to reduce clogging,
minimize sample loss, and extend the
membrane life. Many of Alltech’s syringe filters are
available with 1.0µm borosilicate glass pre-filters.
We highly recommend using syringe filters with
glass pre-filters for particulate-laden samples.
���������������
����������������
��������
�����������������
Syringe Filter with Glass Pre-filter
© Copyright 2004 Alltech Associates, Inc.
Contact your Alltech office or distributor for current or local prices.
27
F I LT R AT I O N
Filtration Introduction
Filter Membrane Chemical Compatibility
Compatible
Limited Compatibility
Not Compatible
No Data Available
REGENERATED CELLULOSE
C
C
C
C
C
C
C
C
L
C
C
C
C
C
C
N
C
N
C
N
C
C
C
C
C
C
C
C
L
C
N
C
C
C
C
C
C
L
C
C
C
C
N
C
C
N
N
-
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
N
C
C
C
C
C
C
C
C
C
N
L
C
C
C
C
C
C
C
C
L
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
-
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
N
N
L
N
N
C
C
C
C
C
N
L
L
L
L
L
C
C
C
C
C
C
C
C
C
L
C
L
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
N
L
N
L
N
N
N
L
C
N
C
N
L
C
L
C
L
C
L
L
L
L
L
L
C
C
L
L
C
C
C
C
C
C
C
© Copyright 2004 Alltech Associates, Inc.
28
REGENERATED CELLULOSE
ANOPORE™
L
N
L
C
N
N
N
L
N
C
C
ANOPORE™
POLYPROPYLENE
C
C
C
C
C
C
N
L
N
C
C
POLYPROPYLENE
CELLULOSE ACETATE
C
C
C
C
C
C
L
L
L
C
C
CELLULOSE ACETATE
POLYSULFONE
C
C
C
C
N
C
C
C
C
C
C
POLYSULFONE
PVDF
L
L
N
L
N
N
N
N
N
N
L
CHEMICAL
Ketones
Acetone
Cyclohexanone
Isopropylacetone
Methyl Ethyl Ketone
Methyl Isobutyl Ketone (MIBK)
Esters
2-Ethoxyethyl Acetate
Amyl Acetate
Benzyl Benzoate
Butyl Acetate
Ethyl Acetate
Isopropyl Myristate
Methyl Acetate
Propyl Acetate
Propylene Glycol Acetate
Methyl Cellosolve Acetate
Tricresyl Phosphate
Oxides — Ethers
Acetonitrile (Methyl Cyanide)
Aniline
Diethyl Acetamide
Dimethyl Formamide
Dimethyl Sulfoxide (DMSO)
Dioxane
Ethyl Ether
Isopropyl Ether
Pyridine
Solvents with Nitrogen
Tetrahydrofuran
Triethanolamine
Miscellaneous
Formaldehyde Solution, 30%
Hydrogen Peroxide, 30%
Phenol, Aqueous, 10%
Silicone Oil & Mineral Oil
PVDF
PTFE
CHEMICAL
Acids
Acetic, 25%
Acetic, Glacial
Formic, 25%
Hydrochloric, 25%
Hydrochloric, Concentrated
Sulfuric, 25%
Sulfuric, Concentrated
Nitric, 25%
Nitric, Concentrated
Phosphoric, 25%
Trichloroacetic, 10%
Bases
Ammonium Hydroxide, 25%
Sodium Hydroxide, 3 Normal
Alcohols
Amyl Alcohol
Benzyl Alcohol
Butyl Alcohol
Ethanol, 70%
Ethanol, 98%
Ethylene Glycol
Glycerine (Glycerol)
Isopropanol
Methanol, 98%
n-Propanol
Propylene Glycol
Hydrocarbons
Benzene
Hexane, Xylene
Kerosene, Gasoline
Tetralin, Decalin
Toluene
Halogenated Hydrocarbons
Carbon Tetrachloride
Chlorobenzene (Mono)
Chloroform
Freon
Methylene Chloride
Trichloroethane
Trichloroethylene
Filter Membrane Chemical Compatibility Chart
NYLON
Filter Membrane Chemical Compatibility Chart
PTFE
=
=
=
=
NYLON
C
L
N
–
C
C
C
C
C
C
C
C
C
C
N
N
N
L
N
N
N
N
N
N
N
N
C
L
C
C
C
C
L
C
C
C
C
C
C
C
C
L
C
C
C
C
C
L
L
L
C
-
L
C
C
C
C
C
C
N
C
C
C
L
C
C
C
L
C
C
-
N
N
N
N
N
N
N
N
N
N
N
L
L
C
L
N
C
N
N
N
N
C
L
L
L
L
L
C
C
-
L
L
C
C
L
C
-
C
C
C
C
C
C
C
C
C
C
C
C
L
C
C
L
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
N
C
L
C
N
L
N
N
N
N
N
C
C
N
N
N
N
N
N
N
L
C
N
C
L
C
C
C
L
C
L
C
L
L
C
C
C
C
C
C
L
L
L
C
C
C
C
C
C
L
C
N
-
N
C
C
-
C
-
L
C
C
N
N
C
C
C
C
C
C
C
L
C
C
C
N
C
L
C
N
C
C
C
C
C
L
L
C
L
C
C
C
This is only a guide. Users should verify
compatibility under actual use conditions.
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Filtration Introduction
Alltech Syringe Filters
More Sizes, More Choices
True™ Syringe Filters
High quality syringe filters with threaded luer inlets,
QC tested for demanding chromatography applications.
New, larger 17mm and 30mm diameters offer higher
sample capacities, faster flow rates, and lower
backpressures. See page 30.
Specialty Syringe Filters
PolyPure™ II syringe filters use a pure, hydrophobic
polypropylene membrane for high solvent resistance and
low extractables. See page 31.
Anotop® syringe filters from Whatman use the Anopore®
membrane; a unique membrane for complete particle exclusion. See page 34.
6277
HPLC Syringe Filters
Traditional 13mm and 25mm diameter syringe filters for common chromatography applications. See pages 32-33.
Economy Syringe Filters
These 25mm syringe filters are an economical alternative for non-aggressive applications. See page 35.
Membrane Selection Guide
Choose a filter membrane based on the size and amount of particulate in the sample, the membrane’s chemical
compatibility with the sample matrix, and potential interactions (binding) between the membrane and the sample
components. This table offers general guidelines on membrane characteristics and applications.
Membrane Selection Guide
MEMBRANE TYPE/ PAGE
Anopore®
Cellulose Acetate
FEATURES
Offers complete particle exclusion.
Contains no glue, binders, plasticizers,
or monomers that can contaminate
the sample.
Ideal for biological applications.
Nylon
Good chemical compatibility and very
low extractables
Polypropylene
(PolyPure™II)
Lower protein binding than PVDF.
Pre-wet with methanol for aqueous
samples
Compatible with strong acids and
aggressive solvents.
Good flow rate characteristics.
Ideal for chromatography applications.
Universal membrane with excellent
chemical resistance and low
extractables. Use for aqueous and
organic samples.
Rated 12-, 30-, or 100kD molecular
weight cut-off. Recover low molecular
weight materials from biological
samples and media.
Polytetrafluoroethylene
(PTFE)
Polyvinylidene Fluoride
(PVDF)
Regenerated Cellulose
Ultrafilters
* Highest solvent resistance.
** Lowest protein binding.
†
PVDF in Syringe Filters is hydrophilic; PVDF in Centrifuge Filter Tubes is hydrophobic.
© Copyright 2004 Alltech Associates, Inc.
COMMON USES
Solvent filtration IC sample
prep biological filtration
HYDROPHILIC
Yes
SOLVENT
RESISTANCE
High
PROTEIN
BINDING
Low
General filtration
sterilization
General filtration
sterilization, HPLC sample
prep
HPLC sample prep
general filtration
Yes
Limited
Very Low
Yes
Good
Medium
No
High
Low
No
High*
High
See Below†
High
Low
Yes
High
Very Low**
Yes
Limited
Low
Gas, air, and solvent
filtration
HPLC sample preparation
and general filtration
HPLC sample prep
general filtration
Sample concentration
deproteinization
buffer exchange
molecule recovery
Contact your Alltech office or distributor for current or local prices.
29
F I LT R AT I O N
Syringe Filters
Alltech True™ Syringe Filters
Larger 17mm and 30mm diameters for:
• Higher sample capacities
• Faster flow rates
• Lower back pressures
Alltech’s new True™ Syringe Filters have true 13mm
and 25mm filter diameters inside larger housings. They
are HPLC Certified for high strength and low extractables.
Larger package sizes are available, and a QC sheet is
included with each unit.
6735
True™ Membrane Specifications
MEMBRANE
MAX. TEMP
Cellulose Acetate
110ºC
Nylon
100ºC
PTFE*
100ºC
PVDF
110ºC
110ºC
Regenerated Cellulose
True PTFE Syringe Filters
™
TYPE
Hydrophilic
Hydrophilic
Hydrophobic
Hydrophilic
Hydrophilic
* True™ PTFE membranes have a polypropylene support layer
True™ Device Specifications
MAX. PRESSURE
DEVICE
4mm
75psig
17mm
115psig
30mm
90psig
HOLD-UP VOLUME
less than 15µL
less than 25µL
less than 115µL
PRICE
True™ Nylon Syringe Filters
MEMBRANE
Nylon
Nylon/Glass
Nylon
Nylon/Glass
Nylon
Nylon/Glass
Nylon
Nylon
Nylon
Nylon
Nylon
TRUE™ NYLON SYRINGE FILTERS
DEVICE
PORE
DIAMETER
SIZE
QTY.
30mm
0.20µm 100
30mm
0.20µm 100
30mm
0.45µm 100
30mm
0.45µm 100
30mm
1.20µm 100
30mm
1.20µm 100
30mm
5.00µm 100
17mm
0.20µm 100
17mm
0.45µm 100
4mm
0.20µm 100
4mm
0.45µm 100
PRICE
True™ PVDF Syringe Filters
True™ Cellulose Acetate Syringe Filters
TRUE™ CELLULOSE ACETATE SYRINGE FILTERS
DEVICE
PORE
MEMBRANE
DIAMETER
SIZE
QTY. PART NO.
Cellulose Acetate
30mm
0.20µm 100
62157
Cellulose Acetate
30mm
0.45µm 100
62187
Cellulose Acetate
17mm
0.20µm 100
62131
Cellulose Acetate
17mm
0.45µm 100
62171
Cellulose Acetate
4mm
0.20µm 100
2634
Cellulose Acetate
4mm
0.45µm 100
2635
MEMBRANE
PTFE
PTFE
PTFE
PTFE
PTFE
PTFE
PTFE
PTFE
TRUE™ PTFE SYRINGE FILTERS
DEVICE
PORE
DIAMETER
SIZE
QTY. PART NO.
30mm
0.20µm 100
62189
30mm
0.45µm 100
62191
30mm
1.00µm 100
62137
30mm
5.00µm 100
62175
17mm
0.20µm 100
62118
17mm
0.45µm 100
62136
4mm
0.20µm 100
2394
4mm
0.45µm 100
2395
MEMBRANE
PVDF
PVDF
PVDF
PVDF
PVDF
PVDF
TRUE™ PVDF SYRINGE FILTERS
DEVICE
PORE
DIAMETER
SIZE
QTY. PART NO.
30mm
0.20µm 100
62193
30mm
0.45µm 100
62195
17mm
0.20µm 100
62130
17mm
0.45µm 100
62209
4mm
0.20µm 100
2227
4mm
0.45µm 100
2228
PRICE
True™ Regenerated Cellulose Syringe Filters
PART NO.
62145
62129
62147
62135
62149
62139
62165
62163
62177
2091
2092
PRICE
TRUE™ REGENERATED CELLULOSE SYRINGE FILTERS
DEVICE
PORE
MEMBRANE
DIAMETER
SIZE
QTY. PART NO.
Regenerated Cellulose
30mm
0.20µm 100 62199
Regenerated Cellulose
30mm
0.45µm 100 62167
Regenerated Cellulose
17mm
0.20µm 100 62119
Regenerated Cellulose
17mm
0.45µm 100 62125
Regenerated Cellulose
4mm
0.20µm 100 656100
Regenerated Cellulose
4mm
0.45µm 100 656102
PRICE
Have a Question about Your Order?
Contact Alltech Customer Service:
Online Ordering 24 hours a day:
www.alltechWEB.com
Email: alltech@alltechemail.com
© Copyright 2004 Alltech Associates, Inc.
30
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Syringe Filters
PolyPure™ II Syringe Filters
• Hydrophobic polypropylene membrane
• Super low extractables
• Contains no wetting agents
POLYPURE™ II SYRINGE FILTERS
DEVICE TYPE, PORE SIZE
QTY.
PART NO.
4mm Syringe Filters
0.20µm
100
6710
0.20µm
500
6712
0.45µm
100
6714
0.45µm
500
6716
13mm Syringe Filters
0.20µm
100
6718
0.20µm
500
6720
0.45µm
100
6722
0.45µm
500
6724
25mm Syringe Filters
0.20µm
100
6730
0.20µm
500
6732
0.45µm
100
6742
0.45µm
500
6744
25mm with Glass Pre-Filter
0.20µm
100
6738
0.20µm
500
6740
0.45µm
100
6750
0.45µm
500
6752
PRICE
4782
PolyPure™ II Specifications
DEVICE
MAX. PRESSURE
4mm
75psig
13mm
75psig
25mm
75psig
MAX. TEMPERATURE HOLD-UP VOLUME
100ºC
less than 7.5µL
100ºC
less than 25µL
100ºC
less than 100µL
Need Technical Assistance?
Contact Alltech Tech Support:
Email: tech_service@alltechemail.com
Online: www.alltechWEB.com
Why are hydrophobic polypropylene membranes better than hydrophilic membranes?
Polypropylene is a naturally hydrophobic filter membrane. Some manufacturers add a wetting
agent to make polypropylene hydrophilic. These chromatograms compare extractables using
isopropanol at 220nm from hydrophilic polypropylene membranes and Alltech’s pure hydrophobic
PolyPure II™ membranes. The large peak present from the hydrophilic polypropylene membrane is
the wetting agent, which elutes in organic solvents and can interfere with your chromatography.
IPA Extraction Off Hydrophilic Polypropylene
0
2
4
6
© Copyright 2004 Alltech Associates, Inc.
8
10
12 Min.
IPA Extraction Off Hydrophobic PolyPure™ II
4612
�
�
�
�
�
�������
4613
Contact your Alltech office or distributor for current or local prices.
31
F I LT R AT I O N
Syringe Filters
Alltech HPLC Syringe Filters
Traditional 13mm and 25mm outer diameter syringe
filters for general purpose chromatography applications.
A QC sheet is included with each unit.
HPLC Membrane Specifications
MEMBRANE
MAX. TEMP
Cellulose Acetate
60ºC
PTFE*
60ºC
Nylon
60ºC
60ºC
Regenerated Cellulose
PVDF
60ºC
TYPE
Hydrophilic
Hydrophobic
Hydrophilic
Hydrophilic
Hydrophilic
* HPLC PTFE membranes have a polypropylene support layer
HPLC Device Specifications
DEVICE
MAX. PRESSURE
13mm
100psig
25mm
100psig
HOLD-UP VOLUME
less than 10µL
less than 50µL
6737
Cellulose Acetate Syringe Filters
PTFE Syringe Filters
• Choose for clarification, sterilization, biological
filtration, and filtration of aqueous solutions
MEMBRANE
13mm
25mm
CELLULOSE ACETATE SYRINGE FILTERS
PORE SIZE
QTY.
PART NO.
0.20µm
100
2642
0.20µm
1000
605025
0.45µm
100
2643
0.45µm
1000
605023
0.20µm
100
2161
0.45µm
100
2160
• Choose for gas, air, and solvent filtration
• Hydrophobic with high solvent resistance
PRICE
Need Help Selecting the
Correct Membrane?
See pages 27-29 for individual
membrane specifications and
suggested use/applications.
MEMBRANE
13mm
25mm
PTFE SYRINGE FILTERS
PORE SIZE
QTY.
0.20µm
100
0.20µm
1000
0.45µm
100
0.45µm
1000
0.20µm
100
0.20µm
1000
0.45µm
100
0.45µm
1000
1.00µm
100
5.00µm
100
PART NO.
2164
605017
2165
605015
2089
605710
2090
605810
2429
6542
PRICE
Need Technical Assistance?
Contact Alltech Tech Support:
Email: tech_service@alltechemail.com
Online: www.alltechWEB.com
4777
© Copyright 2004 Alltech Associates, Inc.
32
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Syringe Filters
Regenerated Cellulose Syringe Filters
Nylon Syringe Filters
• Choose for HPLC sample preparation and general
biological filtration
• High solvent resistance and low protein binding
• Choose for general filtration, sterilization, and
protein binding
NYLON SYRINGE FILTERS
PORE SIZE
QTY.
0.20µm
100
0.20µm
1000
0.45µm
100
0.45µm
1000
25mm
0.20µm
100
0.20µm
1000
0.45µm
100
0.45µm
1000
25mm w/Glass Pre-Filter
0.45µm
100
MEMBRANE
13mm
PART NO.
2166
605013
2167
605011
2045
2945
2047
605410
2359
PRICE
What is the advantage of a glass
pre-filter?
Pre-filters rest on top of the
filter membrane and help trap large
particulates to reduce clogging,
minimize sample loss, and extend the
membrane life. Many of Alltech’s syringe filters are
available with 1.0µm borosilicate glass pre-filters.
We highly recommend using syringe filters with
glass pre-filters for particulate-laden samples.
REGENERATED CELLULOSE SYRINGE FILTERS
MEMBRANE
PORE SIZE
QTY.
PART NO.
13mm
0.20µm
100
656114
0.20µm
1000
605029
0.45µm
100
656116
0.45µm
1000
605027
25mm
0.20µm
100
656124
0.45µm
100
656126
0.45µm
1000
605031
PRICE
PVDF Syringe Filters
• Choose for clarification, sterilization, and biological
filtration
MEMBRANE
13mm
25mm
PVDF SYRINGE FILTERS
PORE SIZE
QTY.
0.20µm
100
0.20µm
1000
0.45µm
100
0.45µm
1000
0.20µm
100
0.45µm
100
PART NO.
2647
605021
2648
605019
2223
2224
PRICE
���������������
Need Larger Diameter
Syringe Filters?
����������������
See Alltech’s new True™ Syringe
Filters on page 30.
��������
6735
�����������������
Need Syringes?
See page 38 for disposable
polypropylene or glass
syringes.
Syringe Filter with Glass Pre-filter
4675
© Copyright 2004 Alltech Associates, Inc.
Contact your Alltech office or distributor for current or local prices.
33
F I LT R AT I O N
Syringe Filters
Anotop® IC Syringe Filters
Anotop® Syringe Filters
• Ideal for ion chromatography - ultra low extractables
• Pigment-free polypropylene housings
• Available with or without
glass pre-filter
• Unique Anopore®
membrane for complete
particle exclusion
4767
Anopore® Membrane Specifications
Anopore®
Membrane:
Hydrophilic
Type:
Low
Protein Binding:
High
Solvent Resistance:
Solvent filtration, IC sample prep,
Common Uses:
biological filtration
Anopore® membranes offer complete
Other Information:
particle exclusion through their unique
capillary pore honeycomb structure.
ANOTOP® SYRINGE FILTERS
DEVICE TYPE, PORE SIZE
QTY.
PART NO.
10mm Syringe Filters
0.02µm
50
2172
0.10µm
50
2240
0.20µm
50
2170
10mm Syringe Filters with Glass Pre-Filter
0.02µm
50
2287
0.10µm
50
2285
0.20µm
50
2283
25mm Syringe Filters
0.10µm
50
2252
0.20µm
50
2130
25mm Syringe Filters with Glass Pre-Filter
0.02µm
50
2262
0.10µm
50
2270
0.20µm
50
2260
Anotop® Syringe Filter Capacities
DESCRIPTION
HOLDUP VOLUME
10mm Syringe Filter
<20µL
25mm Syringe Filter
<150µL
SAMPLE VOLUME
40µL to 10mL
200µL to 100mL
0417
Anopore® Inorganic Membrane
PRICE
0418
Typical Polymeric Membrane
0419
Unique Anopore® Surface Retention
Have a Question about Your Order?
Contact Alltech Customer Service:
Online Ordering 24 hours a day:
www.alltechWEB.com
Email: alltech@alltechemail.com
© Copyright 2004 Alltech Associates, Inc.
34
ANOTOP® IC SYRINGE FILTERS
DEVICE TYPE, PORE SIZE
QTY.
PART NO.
10mm Syringe Filters
0.20µm
50
2370
0.20µm
250
2365
25mm Syringe Filters
0.02µm
50
2132
PRICE
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Syringe Filters
Mini- UniPrep™
Syringeless Filters
Economy Syringe Filters
• A cost effective alternative for general purpose use
• Tabbed luer inlet fitting for stable connections
• Available in 25mm diameter
• Easy all-in-one filtration process
• Fast preparation of HPLC samples
5903
Place your sample in the vial and compress the filter
plunger to get a 12x32mm vial with your filtered sample,
capped and ready to use!
4782
Color-coded polypropylene housings
25MM ECONOMY SYRINGE FILTERS
PORE SIZE
QTY.
PART NO.
0.20µm
100
6837
0.45µm
100
6839
PTFE
0.20µm
100
6821
0.45µm
100
6823
Regenerated Cellulose
0.20µm
100
6829
0.45µm
100
6831
MEMBRANE
Nylon
6760
Need Syringes?
5954
Compression tools make processing Mini-UniPreps fast
and easy.
See page 38 for disposable
polypropylene or glass
syringes.
™
MINI- UNIPREP™ SYRINGELESS FILTERS
DESCRIPTION
QTY.
PART NO.
Clear Mini-UniPrep™
0.2µm Nylon
100
6806
0.45µm Nylon
100
6808
0.2µm PP
100
6817
0.45µm PP
100
6819
0.2µm PTFE
100
6814
0.45µm PTFE
100
6816
Amber Mini-UniPrep™
0.2µm Nylon
100
6832
0.45µm Nylon
100
6834
0.2µm PP
100
6842
0.45µm PP
100
6844
0.2µm PTFE
100
6852
0.45µm PTFE
100
6854
6-Place Compression Tool
ea
6866
1-Place Compression Tool
ea
6861
© Copyright 2004 Alltech Associates, Inc.
PRICE
PRICE
4675
Contact your Alltech office or distributor for current or local prices.
35
F I LT R AT I O N
Centrifuge Filter Tubes
Micro-Spin® Centrifuge
Filter Tubes
Maxi-Spin™ Centrifuge
Filter Tubes
• Economical alternative to expensive, brand name
filter tubes
• For up to 850µL sample volume
• Includes a glass support membrane
• For up to 25mL sample volume
• Use with fixed-angle rotor centrifuge
• All tubes include a glass support membrane
Maxi-Spin™ Centrifuge Filter Tubes remove particulates
from larger sample sizes with any centrifuge that holds
50mL conical tubes. They should only be used with
a fixed-angle rotor centrifuge to prevent premature
clogging of the membrane. Maximum operating
temperature: 50°C. Maximum G-force: 2500xG.
Quickly and easily remove particulates and clarify
small sample volumes. Less than 5µL hold-up volume
provides near quantitative volumetric recovery.
Maximum G-Force: 10,000xG.
4817
4784
ALLTECH MICRO-SPIN® FILTER TUBES
MEMBRANE TYPE, PORE SIZE
QTY.
PART NO.
Cellulose Acetate
0.20µm
100
24126
0.45µm
100
24133
Nylon
0.20µm
100
24137
0.45µm
100
24139
PVDF (Hydrophobic)
0.20µm
100
24142
0.45µm
100
24144
Regenerated Cellulose
0.20µm
100
24148
0.45µm
100
24150
PRICE
MAXI-SPIN® FILTER TUBES
MEMBRANE TYPE, PORE SIZE
QTY.
PART NO.
Cellulose Acetate
0.20µm
50
24152
0.45µm
50
24154
Nylon
0.20µm
50
24156
0.45µm
50
24158
PVDF (Hydrophobic)
0.20µm
50
24160
0.45µm
50
24162
PRICE
Forensic Spin Tubes
• 600µL capacity
• Extract biological fluids from cloth samples
The 1.4mm mesh filter basket acts as a sieve to retain
cloth samples and pass fluids to the receiver tube. The
spin tube includes a 600µL filter basket and a 2mL
microcentrifuge receiver tube with attached cap.
Whatman Ultra-Spin
Centrifuge Filter Tubes
™
• For up to 850µL sample volume
• Operates by molecular weight cut-off
WHATMAN ULTRA-SPIN® FILTER TUBES
MEMBRANE TYPE, MW CUT-OFF
QTY.
PART NO.
Ultrafilter
12kD, Cellulose Triacetate
100
2363
30kD, Polysulfone
100
2369
100kD, Polysulfone
100
2371
© Copyright 2004 Alltech Associates, Inc.
36
4818
PRICE
MAXI-SPIN FILTER TUBES
QTY.
PART NO.
100
2566
250
2567
®
DESCRIPTION
Forensic Spin
Forensic Spin
PRICE
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Filtration Membranes
Alltech Pre-Cut Membranes
• Variety of pore sizes and membrane types
• Non-sterile
ANODISC® PRE-CUT MEMBRANES
DIAMETER
PORE SIZE
QTY.
PART NO.
13mm (no support ring) 0.02µm
100
2935
13mm (no support ring) 0.10µm
100
2933
13mm (no support ring) 0.20µm
100
2253
25mm
0.02µm
50
2255
25mm
0.10µm
50
2268
25mm
0.20µm
50
2250
47mm
0.02µm
50
2235
47mm
0.10µm
50
2276
47mm
0.20µm
50
2230
4777
Anodisc® Membranes
Anodisc® Membranes are hydrophilic and highly
solvent resistant. They will work with both vacuum
and pressure systems, although we recommend using
a membrane support with vacuum systems. Use for
general filtration and sterilization of aqueous and
organic samples. 25mm and 47mm filters are bound to a
polypropylene support ring.
Cellulose Acetate Membranes
Cellulose Acetate Membranes are hydrophilic and
exhibit very low protein retention. Use for general
biological filtration, sterilization, and filtration of
aqueous solutions.
Nylon Membranes
Nylon Membranes are hydrophilic and resistant to
many solvents Use for filtration and clarification of
aqueous or organic solutions.
DIAMETER
13mm
13mm
25mm
25mm
47mm
47mm
CELLULOSE ACETATE PRE-CUT MEMBRANES
PORE SIZE
QTY.
PART NO.
0.20µm
100
2125
0.45µm
100
2126
0.20µm
100
2128
0.45µm
100
2129
0.20µm
100
2133
0.45µm
100
2135
DIAMETER
13mm
13mm
25mm
25mm
47mm
47mm
90mm
90mm
NYLON PRE-CUT MEMBRANES
PORE SIZE
QTY.
PART NO.
0.20µm
100
2046
0.45µm
100
2044
0.20µm
100
2050
0.45µm
100
2048
0.20µm
100
2034
0.45µm
100
2024
0.20µm
25
2540
0.45µm
25
2546
DIAMETER
13mm
13mm
25mm
25mm
25mm
47mm
47mm
47mm
PTFE PRE-CUT MEMBRANES
PORE SIZE
QTY.
PART NO.
0.20µm
100
2013
0.45µm
100
2015
0.20µm
100
2023
0.45µm
100
2029
1.00µm
100
2056
0.20µm
100
2057
0.45µm
100
2058
1.00µm
100
2059
DIAMETER
47mm
47mm
REGENERATED CELLULOSE PRE-CUT MEMBRANES
PORE SIZE
QTY.
PART NO.
0.20µm
100
204700
0.45µm
100
504700
PTFE Membranes
PTFE Membranes are hydrophobic and highly solvent
resistant. Use for filtration of air, gases, and nonaqueous solutions. Pre-treat with methanol before using
with aqueous solutions.
Regenerated Cellulose Membranes
PRICE
PRICE
PRICE
PRICE
PRICE
Need Help Selecting the
Correct Membrane?
Regenerated Cellulose Membranes are hydrophilic and
highly solvent resistant. Use for HPLC sample prep and
general biological filtration.
See pages 27-29 for individual
membrane specifications and
suggested use/applications.
4777
© Copyright 2004 Alltech Associates, Inc.
Contact your Alltech office or distributor for current or local prices.
37
F I LT R AT I O N
Filtration Accessories
Personal Microcentrifuge
Syringes
• For general laboratory use
• Uses luer hub needles – sold separately
• Glass syringes with PTFE tipped plunger are gas and
liquid tight.
• Max. 6000 RPM, 2000xG
force
• Holds six 1.5mL or 2.0mL
tubes
This handy microcentrifuge
fits right at your workstation.
Comes with adapters for
0.5mL tubes.
3424
4675
SYRINGES
DESCRIPTION
QTY.
PART NO.
Glass/PTFE Syringes
1.0mL
1
81320
5.0mL
1
81520
Disposable Polypropylene Syringes with Luer Tip
1.0mL
12
44701100
5.0mL
12
447012
20mL
2
447018
PRICE
Stainless Steel Syringe
Filter Kit
4778
25mm Filter Syringe Kit
Includes SS Filter Holder, 5mL Glass Syringe,
O-Rings, and 50 Anodisc® Membranes
Replacement Parts
13mm Stainless Steel Filter Holder
25mm Stainless Steel Filter Holder
Replacement 13mm O-Rings
Replacement 25mm O-Rings
© Copyright 2004 Alltech Associates, Inc.
38
2060
2038
2042
20196
20198
PERSONAL MICROCENTRIFUGES
PART NO.
22550
22551
PRICE
Polypropylene
Microcentrifuge Tubes
• 17,000xG
• 0.012" piercing port on caps
For our full selection of syringes see
pages 55-71.
ALLTECH SYRINGE FILTER KITS
DESCRIPTION
PART NO.
13mm Filter Syringe Kit
2036
Includes SS Filter Holder, 1mL Glass
Syringe, O-Rings, and 100 each of 0.2µm
and 0.45µm Nylon Membranes
DESCRIPTION
110V
220V, CE Certified
PRICE
POLYPROPYLENE MICROCENTRIFUGE TUBES
DESCRIPTION
COLOR
QTY PART. NO.
0.5mL Standard (no graduations) Natural 1000 22301
Blue
22303
Green
22305
Red
22309
Yellow
22313
Amber
22315
1.5mL Standard (no graduations) Natural 500 22365
Blue
22367
Green
22369
Red
22373
Yellow
22377
Amber
22379
0.5mL Graduated
Natural 1000 22302
Blue
22304
Green
22306
Red
22310
Yellow
22314
Amber
22316
1.5mL Graduated
Natural 500 22385
Blue
22387
Green
22389
Red
22393
Yellow
22397
Amber
22399
2.0mL Graduated
Natural 500 22405
Blue
22407
Green
22409
Red
22413
Yellow
22417
Amber
22419
PRICE
Contact your Alltech office or distributor for current or local prices.
F I LT R AT I O N
Vacuum Filtration
In-Line Vacuum Filtration Systems
Filter Funnels and an In-Line Filter Cap share a
common base to easily accommodate any vacuum
filtration volume.
In-Line
Filter
Cap
Filter funnel has a threaded filter base that connects
directly to any receiver with GL45 threads. It uses any
47mm diameter membrane. To use, connect the filter
base directly to your vacuum source using one of the six
tubing adapters included with each unit. Polypropylene
construction is ideal for mobile phase filtration,
degassing, and culture media filtration. Choose from 1L
and 2L funnel volumes.
Use the In-Line Filter Cap for 100mL to Multi-liter
Filtration tasks.
The In-Line system uses the threaded base of the
funnel system but replaces the funnel with the in-line
filter cap. Tubing is connected to the filter cap and
draws liquid into the system from any container. This
minimizes atmospheric exposure and handling hazards.
The polypropylene fluid path uses any standard 47mm
membrane. Alltech’s built-in priming valve restores
flow in seconds without replacing the membrane or
compromising the flow path.
Base
Use Vacuum Filter Funnels for 100mL to 2L
Filtration tasks.
Base
Filter
Funnel
Filter Funnel
4391
In-Line Filter Cap
4385
VACUUM FILTRATION SYSTEMS
DESCRIPTION
QTY. PART NO.
1L Filter Funnel System
1
6409
Includes Base, Mesh Filter Support,
Adapter Kit, 1L Funnel
2L Filter Funnel System
Includes Base, Mesh Filter Support,
Adapter Kit, 2L Funnel
1
6429
In-Line Filtration System
Includes Base, Mesh Filter Support,
Adapter Kit, In-Line Filter Cap
1
6407
1
1
1
1
1
6473
6463
6418
6438
6421
Replacement Parts
1L Funnel Only
In-Line Filter Cap Only
Replacement Mesh Filter Support Ring
Replacement Adapter Kit
Stainless Steel Mesh Filter Support Ring
PRICE
See Page 26 for Vacuum Pumps.
SuperClean™ SPE Columns for Mobile Phase
Clean-Up
The Super-Clean SPE Column (Figure A) is a 75mL
polypropylene column that contains a blend of carbon
and glass pre-filters to remove particulates and dissolved
organics in a single step. Add a syringe adapter and
1/8" o.d. tubing and connect to the In-Line Filtration
System described above for large volume clean-up
(Figure B) of mobile phases, buffers, and other high
volume samples
SUPERCLEAN™ SPE COLUMNS
DESCRIPTION
QTY.
PART NO.
SuperClean™ SPE Columns
16
29901
Syringe Adapters, 75mL
5
210709
1/8" o.d. PTFE Tubing
10'
20096
© Copyright 2004 Alltech Associates, Inc.
PRICE
5950
Figure A
Figure B
One-step removal of particulates and organics increases
HPLC column lifetime.
5953
Contact your Alltech office or distributor for current or local prices.
39