Genome Size 3000 2500 Size (Mbp) 2000 1500 1000 500 0 yeast fish snake human Sample bird oyster genome Frequency of variant branches 10-1 Frequency of variant branches in the k-de Bruijn graph yeast fish snake human bird oyster genome 10-2 10-3 10-4 10-5 20 30 40 50 k 60 70 80 Frequency of repeat branches 10-1 Frequency of repeat branches in the k-de Bruijn graph yeast fish snake human bird oyster genome 10-2 10-3 10-4 10-5 10-6 20 30 40 50 k 60 70 80 Frequency of error branches 10-1 Frequency of error branches in the k-de Bruijn graph yeast fish snake human bird oyster genome 10-2 10-3 10-4 10-5 20 30 40 50 k 60 70 80 Mean quality score by position 40 35 Mean Phred Score 30 25 20 15 10 5 00 yeast fish snake human bird oyster genome 20 40 60 80 100 Base position 120 140 160 Fraction of bases at least Q30 1.0 Fraction at least Q30 0.9 0.8 0.7 0.6 0.5 0.4 0.30 yeast fish snake human bird oyster genome 20 40 60 80 100 Base position 120 140 160 0.025 Proportion 0.020 k-mer position of first error yeast fish snake human bird oyster genome 0.015 0.010 0.005 0.0000 20 40 60 k-mer Position 80 100 120 Per-position error rate 0.035 yeast fish snake human bird oyster genome 0.030 Error rate 0.025 0.020 0.015 0.010 0.005 0.0000 20 40 60 80 100 base position 120 140 160 0.16 0.14 Duplicate Proportion 0.12 0.10 0.08 0.06 0.04 0.02 0.00 yeast fish snake human Sample bird oyster genome 0.040 Estimated Fragment Size Histogram yeast fish snake human bird oyster genome 0.035 Proportion 0.030 0.025 0.020 0.015 0.010 0.005 0.0000 200 400 600 Fragment Size (bp) 800 1000 51-mer count distribution 0.25 yeast fish snake human bird oyster genome Proportion 0.20 0.15 0.10 0.05 0.000 20 40 60 80 100 51-mer count 120 140 160 yeast GC Bias 100 3200 2800 80 k-mer coverage 2400 60 2000 1600 40 1200 800 20 400 00 20 40 GC % 60 80 100 0 fish GC Bias 100 1400 k-mer coverage 80 1200 1000 60 800 40 600 400 20 200 00 20 40 GC % 60 80 100 0 snake GC Bias 200 2700 2400 k-mer coverage 150 2100 1800 1500 100 1200 900 50 600 300 00 20 40 GC % 60 80 100 0 human GC Bias 100 1400 k-mer coverage 80 1200 1000 60 800 40 600 400 20 200 00 20 40 GC % 60 80 100 0 bird GC Bias 100 2000 1750 80 k-mer coverage 1500 1250 60 1000 40 750 500 20 250 00 20 40 GC % 60 80 100 0 oyster GC Bias 200 1050 900 k-mer coverage 150 750 600 100 450 300 50 150 00 20 40 GC % 60 80 100 0 genome GC Bias k-mer coverage 400 1600 350 1400 300 1200 250 1000 200 800 150 600 100 400 50 200 00 20 40 GC % 60 80 100 0 Simulated contig length N50 50000 Simulated contig lengths in the k-de Bruijn graph yeast fish snake human bird oyster genome 40000 30000 20000 10000 020 30 40 50 60 k 70 80 90 100
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