Guide to Laboratory Services Updated August 2014 Lawrence + Memorial Hospital

Guide to Laboratory Services
Updated August 2014
Lawrence + Memorial Hospital
New London, Connecticut
CT License Numbers
Lawrence + Memorial Hospital Lab HP-0227
Pequot Health Center Lab HP-0263
Cancer Center Lab HP-0352
Table of Contents
PREFACE .................................................................................................... 20
Pathologists: ..................................................................................................................21
Pathologist Assistant: ....................................................................................................21
Administrative Director: ...............................................................................................21
Administrative Consultant: ...........................................................................................21
Section Managers: .........................................................................................................22
Test Priorities ................................................................................................................23
Home Blood Drawing ...................................................................................................24
STAT Laboratory Work ................................................................................................25
Laboratory Critical Results (Panic Values): .................................................................27
L+M Cancer Center Critical Results (Panic Values): ...................................................28
ANATOMIC PATHOLOGY:.................................................................... 29
Slides for Outside Consultation ....................................................................................29
Critical Results ..............................................................................................................29
Specimen Labeling ........................................................................................................29
Specimen Submission/Handling ...................................................................................30
Special Test Request/Submission .................................................................................32
Autopsy (Adult): ...........................................................................................................37
Stillbirth/Fetal Death .....................................................................................................39
BLOOD BANK ........................................................................................... 40
ABO and Rh Typing .....................................................................................................41
ABS see Antibody Screen .............................................................................................42
Albumin.........................................................................................................................43
Ante Partum RhIG, see Rh(D) Immune Globulin Antepartum .....................................44
Antibody Screen ............................................................................................................45
Antibody Titer see Titer ................................................................................................46
Autologous Donors .......................................................................................................47
Autologous Donations are no longer collected at Lawrence + Memorial Hospital.....47
For information on donating Autologous blood, please contact the American Red
Cross, CT region at: 860-678-2805 ..............................................................................47
Blood Typing see ABO and Rh Typing ........................................................................48
Compatibility Testing see Red Blood Cells - Crossmatch ............................................49
Cord Blood ....................................................................................................................50
Cryoprecipitate ..............................................................................................................51
Reference Range: Usual dose is 10 units (2 pools of 5)Cryoprecipitated
Antihemophitic Factor, Cryoprecipitated AHF, CRYO see CryoprecipitateDirect
Antiglobulin Test ..........................................................................................................51
Cryoprecipitated Antihemophitic Factor, Cryoprecipitated AHF, CRYO see
CryoprecipitateDirect Antiglobulin Test .......................................................................52
Direct Antiglobulin Test ...............................................................................................53
DAT see Direct Antiglobulin Test ................................................................................54
Deglyced Red Cells see Red Blood Cells Deglycerolized - Crossmatch......................55
Direct Coombs, see Direct Antiglobulin Test ...............................................................56
FFP see Fresh Frozen Plasma .......................................................................................57
Fresh Frozen Plasma .....................................................................................................58
Frozen Red Cells see Red Blood Cells Deglycerolized - Crossmatch ..........................59
Group & Rh, see ABO and Rh Typing .........................................................................60
HLA-ABC .....................................................................................................................61
HLA-DRDQ ..................................................................................................................62
Indirect Antiglobulin Test, see Antibody Screen ..........................................................63
Indirect Coombs see Antibody screen ...........................................................................64
Packed Red Cells, see Red Blood Cells – Crossmatch Panel, see Antibody
Identification .................................................................................................................65
Pediatric Packed Cells ...................................................................................................66
Phlebotomy see Therapeutic PhlebotomyPlatelet Concentrate .....................................67
Platelet Concentrate ......................................................................................................68
Platelet Packs see Platelet Concentrate .........................................................................69
Pooled Platelets, see PrePooled Platelets .....................................................................70
Prenatal Rhogam see Rh (D) Immune Globulin Antepartum .......................................71
PrePooled Platelets ........................................................................................................72
Random Donor Platelets see Pre-pooled platelets Red Blood Cells - Crossmatch .......73
Red Blood Cells - Crossmatch ......................................................................................74
Red Blood Cells Deglycerolized - Crossmatch .............................................................75
Red Blood Cells Leukocyte Reduced - Crossmatch .....................................................76
Rh Titer, Antibody Titer, Antibody Quantitation, Atypical Antibody Titer see Titers 77
Rh(D) Immune Globulin ...............................................................................................78
Rh(D) Immune Globulin Antepartum ...........................................................................79
RhIG see Rh(D) Immune Globulin ...............................................................................80
RhoGam see Rh(D) Immune Globulin .........................................................................81
Salt Poor Albumin, Normal Serum Albumin, 25% Serum Albumin, 5% Albumin see
Albumin Product ...........................................................................................................82
Therapeutic Bleeding see Therapeutic Phlebotomy ......................................................83
Therapeutic Phlebotomy ...............................................................................................84
Titer ...............................................................................................................................85
Transfusion Reaction Review .......................................................................................86
Type & Screen ...............................................................................................................87
Washed Cells see Red Blood Cells Deglycerolized - Crossmatch ...............................88
Reference Lab.............................................................................................. 89
1st Trimester Risk Screen .............................................................................................90
5-Hydroxyindolacetic Acid, Quantitative, 24 hour Urine .............................................91
5' Nucleotidase, Blood ..................................................................................................92
17-Hydroxyprogesterone ...............................................................................................93
17-Ketosteroids, Total, 24 hour Urine ..........................................................................94
Acetylcholine Receptor Antibody .................................................................................95
Adenovirus Titer ...........................................................................................................96
Adrenocorticotrophic Hormone ....................................................................................97
Aldolase, Blood .............................................................................................................99
Aldosterone, 24 hour Urine .........................................................................................100
Aldosterone, Serum .....................................................................................................101
Alkaline Phosphatase Isoenzymes, Blood ...................................................................102
Alkaline Phosphatase, Bone Specific ..........................................................................103
Alpha - 1 - Antitrypsin, Blood ....................................................................................104
Alpha feto protein, maternal .......................................................................................105
Alprazolam ..................................................................................................................106
Aluminum, Blood ........................................................................................................107
Amino Acids - Quantitative Plasma ............................................................................108
Amino Acids - Quantitative Urine ..............................................................................109
Amino Levulinic Acid, 24 hour Urine ........................................................................110
Amiodarone .................................................................................................................111
Amitriptyline ...............................................................................................................112
Amniotic Fluid L/S Ratio ............................................................................................113
Amphetamines, Urine .................................................................................................114
Amylase Isoenzymes ...................................................................................................115
Anaplasma phagocytophilum Antibody ......................................................................116
Androstenedione .........................................................................................................117
Angioedema Panel, Acquired......................................................................................118
Angiotensin Converting Enzyme ................................................................................119
Anti-Cardiolipin Antibody ..........................................................................................120
Antidiuretic Hormone .................................................................................................121
Anti-DNA, Single Stranded ........................................................................................122
Anti Strep DNase-B ....................................................................................................123
Anti-Epidermal Antibody............................................................................................124
Anti-Glomerular Basement Membrane .......................................................................125
Anti-Mitochondrial Antibody .....................................................................................126
Anti-Neutrophil Cytoplasmic Antibody ......................................................................127
Antinuclear Antibody ..................................................................................................128
Reference Range: < 1:40. ANA pattern reported with positive results.......................128
Antiplatelet Antibody ..................................................................................................129
Anti-Streptolysin O Titer ............................................................................................130
Apolipoprotein A1 ......................................................................................................131
Apolipoprotein B .........................................................................................................132
Apolipoproteins A1&B ...............................................................................................133
Acetylcholine Blocking Antibody...............................................................................134
Arsenic, 24 hour urine .................................................................................................135
Arsenic, Blood.............................................................................................................136
ASCA (Saccharomyces Cerevisiae) IgA ....................................................................137
ASCA (Saccjaromyces Cerevisiae) IgG .....................................................................138
Aspergillus fumigatus IgG ..........................................................................................139
Aspergillus, Serology ..................................................................................................140
Babesia Serology .........................................................................................................141
Barbiturates, Total, Blood ...........................................................................................142
Beta-2-Glycoprotein (IgG, IgA, IgM) .........................................................................143
Beta-2-Microglobulin ..................................................................................................144
Blastomyces, Serology ................................................................................................145
Blood Chromosome Analysis .....................................................................................146
Chromosomes, Bone Marrow or Peripheral Blood .....................................................147
Bordetella Pertussis Antibodies ..................................................................................148
C1 Esterase, Functional ...............................................................................................149
C1 Esterase Inhibitor ...................................................................................................150
C3d Circulating Immune Complexes ..........................................................................151
CA15-3 ........................................................................................................................152
CA19-9 ........................................................................................................................153
CA27.29 ......................................................................................................................154
Caffeine .......................................................................................................................155
Calcitonin ....................................................................................................................156
Candida albicans Antibodies .......................................................................................157
Carbamazepine 10, 11 Epoxide...................................................................................158
Carotene ......................................................................................................................159
Cat Scratch Fever Serology .........................................................................................160
Catecholamines, 24 hour Urine ...................................................................................161
Catecholamines, plasma ..............................................................................................162
Celiac DNA Genotyping .............................................................................................163
Ceruloplasmin .............................................................................................................164
Cholinesterase, Blood .................................................................................................165
Chromium, Plasma ......................................................................................................166
Chromogranin A..........................................................................................................167
Chromosome Analysis, Tissue ....................................................................................168
Citrate, 24 hour urine ..................................................................................................169
Clonazepam .................................................................................................................172
Clozapine.....................................................................................................................173
CMV Antigenemia ......................................................................................................174
Cocaine Metabolites, Urine .........................................................................................176
Coccidioides Antibody ................................................................................................177
Coenzyme Q10 ............................................................................................................178
Cold Agglutinins .........................................................................................................179
Collagen Type I C-Telopeptide (CTx) ........................................................................180
Collagen Cross-Linked N-Telopeptide, Urine ............................................................181
Complement, C2 .........................................................................................................182
Complement, C5 .........................................................................................................183
Complement, C6 .........................................................................................................184
Complement, Total (CH5O)........................................................................................185
Copper, 24 hour urine .................................................................................................186
Copper, Blood .............................................................................................................187
Cortisol, Free 24 hour urine ........................................................................................188
Coxsackie B Virus Titer ..............................................................................................189
C-Peptide .....................................................................................................................190
Creatine Kinase Isoenzyme Panel ...............................................................................191
Cryoglobulins ..............................................................................................................192
Cryptococcus Antibody ...............................................................................................193
CSF IgG ......................................................................................................................194
Cyclic Citrulline Peptide, IgG .....................................................................................195
Cyclosporine ...............................................................................................................197
Cystic Fibrosis Screen .................................................................................................198
Cystine, 24 hour urine .................................................................................................199
Cytomegalovirus Antibodies .......................................................................................200
Desipramine ................................................................................................................202
DHEA Sulfate .............................................................................................................203
Diphtheria Antitoxoid Antibody .................................................................................204
DISOPYRAMIDE.......................................................................................................205
Drug Screen, Serum Only ...........................................................................................207
Echinococcus Serology ...............................................................................................208
Endomysial IgA Antibody ..........................................................................................209
Epstein-Barr Virus DNA, Quantitative PCR ..............................................................212
Erythropoietin .............................................................................................................213
Estrone, serum .............................................................................................................214
Extractable Nuclear Antigen Antibodies ....................................................................215
Factor V Leiden Mutation Analysis ............................................................................216
Factor VIII Multimers .................................................................................................217
Factor VIII Related Antigen ........................................................................................218
Fecal Fat, Random ......................................................................................................219
Fecal Fat, Timed specimen .........................................................................................220
Flecainide ....................................................................................................................221
Flow Cytometry, Bone Marrow or Peripheral Blood..................................................222
Folate, RBC .................................................................................................................223
Fragile-X .....................................................................................................................224
Free T3 ........................................................................................................................225
Free Testosterone ........................................................................................................226
Fructosamine ...............................................................................................................227
FTA-CSF .....................................................................................................................228
FTA Profile .................................................................................................................229
Fungal Serology Panel ................................................................................................230
Gabapentin ..................................................................................................................231
Ganglioside GM-1 Antibodies ....................................................................................232
Gastric Parietal Cell Antibody ....................................................................................233
Gastrin .........................................................................................................................234
Glucagon .....................................................................................................................235
Glucose-6-Phosphate Dehydrogenase .........................................................................236
Glutamic Acid Decarboxylase-65 Antibody ...............................................................237
Growth Hormone (HGH), Human ..............................................................................238
Haptoglobin .................................................................................................................239
Heavy Metal Screen, blood .........................................................................................240
Helicobacter Antibodies ..............................................................................................241
Hemoglobin Electrophoresis .......................................................................................242
Heparin Anti-Xa LMWH Assay .................................................................................243
Heparin Induced Platelet Antibody .............................................................................244
Hepatitis A Antibody, Total with Reflex to IgM ........................................................245
Hepatitis B E Antibody ...............................................................................................246
Hepatitis B E Antigen .................................................................................................247
Hepatitis B Surface Antigen, Neutralization ...............................................................248
Hepatitis B Viral (HBV) DNA Quantitative by PCR .................................................249
Hepatitis C Genotype ..................................................................................................250
Hepatitis C RNA .........................................................................................................251
Hereditary Hemochromatosis, DNA, Mutation Analysis ...........................................252
Herpes Simplex Virus Antibodies ...............................................................................253
Herpes Simplex Virus (HSV) IgM Antibody with Reflex to Titer .............................256
Histamine, blood .........................................................................................................261
Histone Antibodies ......................................................................................................262
Histoplasma Antibody .................................................................................................263
HIV, RNA by PCR (Ultra) ..........................................................................................266
HIV Genotype .............................................................................................................267
HIV-1 Phenotype ........................................................................................................268
HLA - B27...................................................................................................................269
HTLV ABS I & II .......................................................................................................270
Hypersensitivity Pneumonitis Evaluation ...................................................................271
Hypersensitivity Pneumonitis Screen .........................................................................272
IDPH see Immune Deficiency Panel IGF (Insulin-like growth factor) Binding Protein2 ...................................................................................................................................273
IGF (Insulin-like growth factor) Binding Protein-2 ....................................................274
IGF (Insulin-like growth factor) Binding Protein-3 ....................................................275
IgG (Immunoglobulin G) Subclass 4 ..........................................................................276
IgG Subclass Fractionation .........................................................................................277
Imipramine ..................................................................................................................278
Immune Complex, C1 Q .............................................................................................279
Immune Complex Panel ..............................................................................................280
Immune Deficiency Panel ...........................................................................................281
Immunoelectrophoresis, Serum...................................................................................282
Immunoglobulin D ......................................................................................................283
Influenza Antibody Panel ............................................................................................284
Insulin Antibody (Insulin Auto-antibody) ..................................................................285
Insulin Growth Factor-1 ..............................................................................................286
Intrinsic factor Antibody .............................................................................................287
Islet Cell Antibody ......................................................................................................288
JAK2 Mutation ............................................................................................................289
Jo-1 Antibody ..............................................................................................................290
Keppra .........................................................................................................................291
Lamictal.......................................................................................................................292
Lead, Blood .................................................................................................................293
Legionella Serology ....................................................................................................294
Lipoprotein A ..............................................................................................................295
Lipoprotein Fractionation Panel 2, Ion Mobility ........................................................296
Lipoprotein Electrophoresis ........................................................................................297
Liver-Kidney Microsomal Antibody (IgG) .................................................................298
Lorazepam ...................................................................................................................299
Lyme Disease Antibody with Reflex to CSF ratio ......................................................300
Lyme Titer, CSF..........................................................................................................301
Lyme Titer, Misc Fluid ...............................................................................................302
Lyme - Western blot....................................................................................................303
Lymphocyte Enumeration Panel .................................................................................304
Lymphocytic Choriomeningitis Virus Antibody ........................................................305
Lysozyme ....................................................................................................................306
Magnesium, RBC ........................................................................................................307
Magnesium, Urine 24 hour .........................................................................................308
Magnesium, Urine Random ........................................................................................309
Mephobarital Level .....................................................................................................310
MecSTAT 7 panel .......................................................................................................311
MecSTAT 9 panel .......................................................................................................312
Mercury, blood ............................................................................................................313
Metanephrines, Plasma ...............................................................................................314
Metanephrines, Total, 24 hour Urine ..........................................................................315
Methadone, urine.........................................................................................................316
Methotrexate Level .....................................................................................................317
Methylenetetrahydrofolate Reductase .........................................................................318
Methylmalonic Acid....................................................................................................319
MTHFR see Methylenetetrahydrofolate Reductase ....................................................320
Mumps Antibodies ......................................................................................................323
Myasthenia Gravis Panel.............................................................................................324
Mycoplasma Serology .................................................................................................325
Myelin Basic Protein ...................................................................................................326
Myoglobin ...................................................................................................................327
Myoglobin, Urine ........................................................................................................328
Mysoline, Level...........................................................................................................329
Niemann-Pick Disease Mutation Analysis ..................................................................332
Nortriptyline ................................................................................................................333
Organic Acids, urine ...................................................................................................334
Oxalate, Urine .............................................................................................................336
Paraneoplastic AB Evaluation.....................................................................................337
Parvovirus titers ..........................................................................................................338
Pentobarbital ...............................................................................................................339
Phencyclidine ..............................................................................................................340
Phenylalanine, PKU ....................................................................................................341
Phenytoin, Free ...........................................................................................................342
Phosphatidylserine Antibodies (IgG, IgM) .................................................................343
Phospholipids ..............................................................................................................344
PNH with FLAER (High Sensitivity) .........................................................................345
Poliovirus Antibody, Neutralization ...........................................................................346
Porphobilinogen, 24 hour urine...................................................................................347
Porphyrins, Fractionated, Quantitative and Porphobilinogen, 24-Hour Urine ...........348
Porphyrins, Fractionated, Quantitative, Random Urine ..............................................349
Primadone ...................................................................................................................350
Prograf .........................................................................................................................351
Pro-Predict Metabolites ...............................................................................................352
Protein Electrophoresis, Serum ...................................................................................353
Protein Immunoelectrophoresis, Urine .......................................................................354
Prothrombin Gene Mutation .......................................................................................355
PTH, C Terminal .........................................................................................................356
PSA, Free and Total ....................................................................................................357
Pyruvic Acid................................................................................................................358
Q Fever, Titer ..............................................................................................................359
Quinidine, Blood .........................................................................................................360
Rapamycin ...................................................................................................................361
Renin Activity .............................................................................................................362
Reticulin Antibody, IgA ..............................................................................................365
Ristocetin Co-Factor ...................................................................................................366
Rubella Antibodies ......................................................................................................367
Rubeola Antibodies .....................................................................................................368
Schistosoma Serology .................................................................................................369
Scleroderma Antibody ................................................................................................370
Sex Hormone Binding Globulin..................................................................................371
Serotonin, Blood .........................................................................................................372
Sjogrens Antibodies ....................................................................................................373
Smooth Muscle Antibody ...........................................................................................374
Somatostatin ................................................................................................................375
Stone Analysis .............................................................................................................376
Striated Muscle Antibodies .........................................................................................377
Rickettsia Antibodies ..................................................................................................378
Stool Osmolality, 24 hour ...........................................................................................379
Stool Potassium, 24 hour.............................................................................................380
Stool Sodium, 24 hour.................................................................................................381
Streptococcus pneumoniae IgG (23 Serotypes) ..........................................................382
Streptozyme.................................................................................................................383
Tay-Sachs Detection ...................................................................................................384
Tay-Sachs Gene Mutation Analysis -Tay Sachs Mutation .........................................385
Tetanus Antitoxoid Antibody ......................................................................................386
THC Confirmation, Urine ...........................................................................................387
ThinPrep Pap and HR HPV DNA ...............................................................................388
ThinPrep Pap with reflex to HR HPV DNA ...............................................................389
ThinPrep Imaging Pap .................................................................................................390
Thrombin Time ...........................................................................................................391
Thyroglobulin ..............................................................................................................392
Thyroglobulin Antibodies ...........................................................................................393
Thyroid Auto-Antibodies ............................................................................................394
Thyroid Peroxidase Ab ...............................................................................................395
Thyroid Stimulating Immunoglobulin ........................................................................396
Thyroxine Binding Globulin .......................................................................................397
Tissue Transglutaminase Antibody, IgG .....................................................................398
Topamax ......................................................................................................................399
Torch Panel .................................................................................................................400
Toxicology Profile, Blood (Tox. Screen, Blood) ........................................................401
Toxocara ......................................................................................................................402
Toxoplasmosis Antibodies ..........................................................................................403
Toxoplasmosis, Status .................................................................................................404
TPMT ..........................................................................................................................405
TPMT (Thiopurine S-Methyltransferase) Genotype ...................................................406
Transferrin ...................................................................................................................407
Trileptal Level .............................................................................................................408
Tryptase .......................................................................................................................409
TT see Thrombin Time ...............................................................................................410
Urine Myoglobin .........................................................................................................411
Vanillylmandelic Acid, 24 Hour Urine .......................................................................412
VAP ™ Cholesterol panel ...........................................................................................413
Varicella Status ...........................................................................................................414
Varicella-Zoster, Antibodies .......................................................................................415
Vasoactive Intestinal Peptide ......................................................................................416
VDRL-CSF .................................................................................................................417
Viscosity, Serum .........................................................................................................419
Vitamin A, Blood ........................................................................................................420
Vitamin B1 ..................................................................................................................421
Vitamin B2 ..................................................................................................................422
Vitamin B6 ..................................................................................................................423
Vitamin B12 Binding Capacity, Unsaturated..............................................................424
Vitamin C ....................................................................................................................425
Vitamin D1, 25-Dihydroxy .........................................................................................426
Vitamin D, 25 Hydroxy fractionation .........................................................................427
Vitamin E ....................................................................................................................428
Vitamin K ....................................................................................................................429
Zinc, Blood ..................................................................................................................430
CHEMISTRY ............................................................................................ 432
Acetaminophen, Blood ................................................................................................433
Acetone, Quantitative, Blood ......................................................................................434
Albumin, Blood ...........................................................................................................435
Albumin, Fluid ............................................................................................................436
Alcohol, Blood ............................................................................................................437
Alcohol, Urine .............................................................................................................438
Alkaline Phosphatase, Blood ......................................................................................439
Alpha feto protein, tumor marker ................................................................................440
Ammonia, Blood .........................................................................................................441
Amniotic Fluid L/S Ratio ............................................................................................442
Amylase, Blood ...........................................................................................................443
Amylase, Body Fluids .................................................................................................444
Amylase, Urine random ..............................................................................................445
Anti-Centromere Antibody .........................................................................................446
Anti-Gliadin Antibodies, IgA and IgG ........................................................................447
Arterial Blood Gas ......................................................................................................448
Arterial pH ..................................................................................................................449
Basic Metabolic Panel .................................................................................................450
Bilirubin, Direct ..........................................................................................................451
Bilirubin, Total (Neonatal) ..........................................................................................452
Bilirubin, Total and Direct, Blood ..............................................................................453
Blood Oxygen Saturation ............................................................................................454
BNP .............................................................................................................................455
C3 Complement ..........................................................................................................456
C4 Complement ..........................................................................................................457
CA-125 ........................................................................................................................458
Calcium, Blood ...........................................................................................................459
Calcium, Serum, Ionized .............................................................................................460
Calcium, Urine ............................................................................................................461
Carbon Dioxide, Total .................................................................................................462
Carboxyhemoglobin ....................................................................................................463
Carcinoembryonic Antigen .........................................................................................464
CCU Profile .................................................................................................................465
Celiac Profile ...............................................................................................................466
Childhood Allergy Panel .............................................................................................467
Chloride, Blood ...........................................................................................................468
Chloride, CSF ..............................................................................................................469
Chloride, Urine ............................................................................................................470
Cholesterol, Total ........................................................................................................471
Cholesterol, Fluid ........................................................................................................472
Cholesterol Fractionation ............................................................................................473
CKMB (Creatine Kinase, MB Fraction) .....................................................................474
Comprehensive Inhalant Panel ....................................................................................475
Comprehensive Metabolic Panel.................................................................................476
Cortisol ........................................................................................................................477
CPK .............................................................................................................................478
C-Reactive Protein ......................................................................................................479
C-Reactive Protein, Cardiac ........................................................................................480
Creatinine, Blood ........................................................................................................481
Creatinine Clearance ...................................................................................................482
Creatinine, 24 hour Timed or Random Urine .............................................................483
Dilantin, Blood ............................................................................................................485
Drugs of Abuse, Urine ................................................................................................486
Electrolytes, Blood ......................................................................................................487
Electrolytes, Urine.......................................................................................................488
Estradiol ......................................................................................................................489
Ferritin, serum .............................................................................................................490
Folate, serum ...............................................................................................................491
Follicle Stimulating Hormone .....................................................................................492
General Food Screen ...................................................................................................494
GGT.............................................................................................................................495
Gentamicin ..................................................................................................................496
Glucose, Blood ............................................................................................................497
Glucose, Fluid .............................................................................................................498
Glucose, OB screen .....................................................................................................499
Glucose, Spinal Fluid ..................................................................................................500
Glucose Tolerance OB Patients ..................................................................................501
Glucose Tolerance Test 2 Hours .................................................................................502
Glycosylated Hemoglobin ...........................................................................................503
HCG, male...................................................................................................................504
HCG, Quantitative, Beta Subunit ................................................................................505
Heart Profile ................................................................................................................506
Hemoglobin, Total ......................................................................................................507
Hepatitis A Antibody (IgM) ........................................................................................508
Hepatitis B Core Antibody (IgM) ...............................................................................509
Hepatitis B Surface Antibody .....................................................................................510
Hepatitis B Surface Antigen ........................................................................................511
Hepatitis C Virus Antibody .........................................................................................512
Hepatitis Panel ............................................................................................................513
HIV-1 Antibody ..........................................................................................................514
Homocysteine ..............................................................................................................515
IgE ...............................................................................................................................516
Immunoglobulin Panel ................................................................................................517
Infectious Mononucleosis Test ...................................................................................518
Insulin Glucose Tolerance, 2 or 3 Hours ....................................................................519
Insulin, Random ..........................................................................................................520
Iron Profile ..................................................................................................................521
Lactic Acid, Blood ......................................................................................................522
Lactic Acid, CSF .........................................................................................................523
Lactic Dehydrogenase, Blood .....................................................................................524
Lactic Dehydrogenas, Fluid ........................................................................................525
Lactose Tolerance Test ...............................................................................................526
Latex Allergen .............................................................................................................527
LDL cholesterol ..........................................................................................................528
Lipase, Serum ..............................................................................................................529
Lithium, Blood ............................................................................................................530
Liver Profile ................................................................................................................531
Luteinizing Hormone ..................................................................................................532
Lyme Disease Serology, Serum ..................................................................................533
Magnesium, Blood ......................................................................................................534
Measles Immunity Panel .............................................................................................535
Methemoglobin ...........................................................................................................536
Microalbumin, Urine ...................................................................................................537
New England Regional Inhalant Panel .......................................................................538
Nut Panel .....................................................................................................................539
Osmolality, Serum.......................................................................................................540
Osmolality, Urine ........................................................................................................541
PG - FLM Test ............................................................................................................542
pH, Body Fluid ............................................................................................................543
Phenobarbital, Blood ...................................................................................................544
Phosphate, Urine .........................................................................................................545
Phosphorus, Blood ......................................................................................................546
Potassium, Blood.........................................................................................................547
Potassium, Urine .........................................................................................................548
Prealbumin ..................................................................................................................549
Pregnancy Test, Serum................................................................................................550
Pregnancy Test, Urine .................................................................................................551
Prenatal Profile ............................................................................................................552
Procalcitonin ...............................................................................................................553
Progesterone ................................................................................................................554
Prolactin ......................................................................................................................555
Prostate Specific Antigen ............................................................................................556
Protein, Body Fluid .....................................................................................................557
Protein, CSF ................................................................................................................558
Protein, Total, Blood ...................................................................................................559
Protein, Urine ..............................................................................................................560
PTH, intact panel .........................................................................................................561
RAST testing – miscellaneous tests ............................................................................562
Rheumatoid Factor ......................................................................................................563
Rheumatoid Profile .....................................................................................................564
Rubella, Status.............................................................................................................565
Rubeola, Status ............................................................................................................566
Salicylate, Blood .........................................................................................................567
SGOT/AST ..................................................................................................................568
SGPT/ALT ..................................................................................................................569
Shellfish Panel .............................................................................................................570
Sodium, Blood.............................................................................................................571
Sodium, Urine .............................................................................................................572
Stinging Insect Panel ...................................................................................................573
Syphyllis Antibodies, Blood .......................................................................................574
T-3, Total.....................................................................................................................576
T4, Free .......................................................................................................................577
Tegretol .......................................................................................................................578
Testosterone, Total ......................................................................................................579
Theophylline ...............................................................................................................580
Thyroid Profile ............................................................................................................581
Thyroid Stimulating Hormone, Ultra sensitive ...........................................................582
Tissue Transglutaminase IgA ......................................................................................583
Triglycerides, Blood ....................................................................................................586
Triglycerides – Fluid ...................................................................................................587
Troponin I....................................................................................................................588
Urea Nitrogen, Blood ..................................................................................................589
Urea, Urine ..................................................................................................................590
Uric Acid, Blood .........................................................................................................591
Uric Acid, Fluid ..........................................................................................................592
Uric Acid, Urine ..........................................................................................................593
Valproic Acid ..............................................................................................................594
Vitamin B12 ................................................................................................................596
Vitamin D 25-Hydroxy ...............................................................................................597
COAGULATION ...................................................................................... 598
Activated Partial Thromboplastin Time ......................................................................599
Antithrombin III ..........................................................................................................600
APTT see Activated Partial Thromboplastin Time .....................................................601
C.A.C. Circulating Anticoagulant see: Mixing Studies ..............................................602
Coagulation Factor Assays see Factor VIII Assay ......................................................603
Coagulation Profile .....................................................................................................604
D-Dimer Quantative ....................................................................................................605
D-Dimer ......................................................................................................................606
DIC Profile ..................................................................................................................607
Factor II Mutation see Prothrombin Gene Mutation ...................................................608
Factor VIII Assay ........................................................................................................609
Fibrinogen Level .........................................................................................................610
HCOAG see Hypercoagulable profile ........................................................................611
Hemorrhagic Screen see Coagulation Profile .............................................................612
Hypercoagulable Profile ..............................................................................................613
LUPA see Lupus Anticoagulant..................................................................................614
Lupus Anticoagulant ...................................................................................................615
Mixing Studies ............................................................................................................616
PFA see Platelet Function Assay ................................................................................617
Platelet Function Assay ...............................................................................................618
Protein C......................................................................................................................619
Protein S ......................................................................................................................620
Prothrombin Time/INR ...............................................................................................621
PT/INR see Prothrombin Time/INR ...........................................................................622
CYTOLOGY ............................................................................................. 623
Ascitic Fluid Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial .624
Breast Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc. ..................................................................................................................625
Bronchial Brushings, Cytology see Cytology, Brushings: Bronchial, Esophageal,
Gastric, Small Bowel, Colonic, Etc. ...........................................................................626
Bronchial Washings, Cytology see Cytology, Bronchial, Esophageal Washings and
Tracheal Aspirations ...................................................................................................627
Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc. ..................................................................................................................628
Cytology, Body Fluids: Pleural, Ascitic and Pericardial ............................................629
Cytology, Bronchial, Esophageal Washings and Tracheal Aspirations ......................630
Cytology, Brushings: Bronchial, Esophageal, Gastric, Small Bowel, Colonic, Etc. ..631
Cytology, Cervical – Vaginal Smears .........................................................................632
Cytology, Nipple Discharge ........................................................................................633
Cytology, Spinal and Cyst Fluid: Renal, Ovarian, Breast, Etc. ..................................634
Cytology, Sputum .......................................................................................................635
Cytology, Urine ...........................................................................................................636
Cytology: Viral Study: Cytomegalic Virus on Urine ..................................................637
Cytomegalic Inclusion Bodies see Cytology: Viral Study: Cytomegalic Virus on
Urine ............................................................................................................................638
Cytomegalic Inclusion Disease (CID) see Cytology: Viral Study: Cytomegalic Virus
on Urine .......................................................................................................................639
Esophageal Washings, Tracheal Aspiration Cytology see Cytology, Bronchial,
Esophageal Washings and Tracheal Aspirations ........................................................640
Fine Needle Aspiration ...............................................................................................641
Nipple Discharge Cytology, see Cytology, Nipple Discharge ....................................642
Ovarian Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc. ..................................................................................................................643
Pap Smear see Cytology, Cervical - Vaginal Smears .................................................644
Papanicolaou Smear see Cytology, Cervical - Vaginal Smears ..................................645
Paracentesis Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial ..646
Pericardial Fluid Cytology see Cytology, Body Fluids: Pleural, Ascitic and
Pericardial ...................................................................................................................647
Pericardiocentesis Cytology see Cytology, Body Fluids: Pleural, Ascitic and
Pericardial ...................................................................................................................648
Pleural Fluid Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial .649
Renal Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc. ..................................................................................................................650
Spinal Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian, Breast,
Etc. ..............................................................................................................................651
Sputum Cytology see Cytology, Sputum ....................................................................652
Sputum Series Cytology see Cytology, Sputum .........................................................653
Thoracentesis Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial654
Tzanck Preparation......................................................................................................655
HEMATOLOGY ...................................................................................... 656
Babesia Smear .............................................................................................................657
Body Fluid Analysis ....................................................................................................658
Body Fluid ...................................................................................................................659
Bone Marrow ..............................................................................................................660
CBC see Complete Blood Count.................................................................................661
Chronic Leukemia/Lymphoma Immunophenotyping .................................................662
Cerebrospinal Fluid Analysis ......................................................................................663
Complete Blood Count ................................................................................................664
Complete Blood Count with Manual Differential .......................................................665
CSFCT see Cerebrospinal Fluid Analysis...................................................................666
Ehrlichia Smear ...........................................................................................................667
Eosinophil Smear ........................................................................................................668
Erythrocyte Sedimentation Rate see Sedimentation Rate ...........................................669
ESR see Sedimentation Rate .......................................................................................670
FCEL see Body Fluid Analysis ...................................................................................671
Fetal Hemoglobin Stain...............................................................................................672
FHCT see Hematocrit - Body Fluid ............................................................................673
Fluid Crystals ..............................................................................................................674
Fluid Hematocrit .........................................................................................................675
Hemogram see Complete Blood Count.......................................................................676
Hematocrit - Peripheral Blood ....................................................................................677
Hemoglobin-Peripheral Blood ....................................................................................678
Hemogram see Complete Blood Count.......................................................................679
Iron Stain, Bone Marrow - see Bone Marrow ............................................................680
Kleihauer-Betke Fetal Hgb Stain - see Fetal Hemoglobin Stain................................681
Malaria Smear .............................................................................................................682
Manual Differential see Differential White Blood Cell Count ...................................683
Nasal Smear for Eosinophils see Eosinophil Smear ...................................................684
Platelet Count ..............................................................................................................685
Retic Count see Reticulocyte Count ...........................................................................686
Reticulocyte Count ......................................................................................................687
RTC see Reticulocyte Count .......................................................................................688
Sedimentation Rate .....................................................................................................689
Sed Rate see Sedimentation Rate ................................................................................690
Sickle see Sickle Cell Preparation ...............................................................................691
Sickle Cell see Sickle Cell Preparation .......................................................................692
Sickle Cell Preparation ................................................................................................693
Spinal Fluid Cell Count see Cerebrospinal Fluid Analysis .........................................694
Sputum for Eosinophils see Eosinophil Smear ...........................................................695
Synovial Fluid Analysis ..............................................................................................696
WBC see White Blood Cell Count..............................................................................697
White Blood Cell Count ..............................................................................................698
HOME BLOOD DRAWING ................................................................... 699
MICROBIOLOGY ................................................................................... 700
MICROBIOLOGY COVERAGE ...............................................................................700
MICROBIOLOGY REQUISITIONING ....................................................................700
COLLECTION AND TRANSPORT OF MICROBIOLOGY SPECIMENS ............700
REJECTION OF SPECIMENS ..................................................................................702
SPECIMEN COLLECTION .......................................................................................703
Stool Collection/Transport Guide...........................................................................708
Recommendation for O&P evaluation ........................................................................708
AFB Blood Culture see Culture, Mycobacteria, Blood ..............................................710
AFB Smear and Culture, MAV Culture, TB Culture see Culture, Mycobacteria.......711
Beta Strep Group A (Rapid Test) ...............................................................................712
Beta Strep Screen (24 hour) see Culture, Throat, for Beta Strep Group A .................713
Blood Culture for MAV see Culture, Mycobacteria, Blood .......................................714
Blood Culture, Fungus see Culture, Blood .................................................................715
Blood Culture, Routine see Culture, Blood ................................................................716
C. Difficile Toxin ........................................................................................................717
Candida Antigen ..........................................................................................................718
Chlamydia PCR ...........................................................................................................719
Clostridium Difficile Toxin, Stool for C.Diff see C. Difficile Toxin .........................720
Cryptococcal Antigen, Blood ......................................................................................721
Cryptococcal Antigen, CSF ........................................................................................722
Cryptococcus Stain see India Ink Prep ........................................................................723
Cryptosporidum Antigen .............................................................................................724
Culture, Anaerobic ......................................................................................................725
Culture, Candida .........................................................................................................726
Culture, Anal/Rectal for GC .......................................................................................727
Culture, Blood – Bacterial...........................................................................................728
Culture, Body Fluid .....................................................................................................729
Culture, Bone ..............................................................................................................730
Culture, Bone Marrow ................................................................................................731
Culture, Bone Marrow for AFB see Culture, Mycobacteria, Bone Marrow ...............732
Culture, Bronchial Washing ........................................................................................733
Culture, Catheter Tip ...................................................................................................734
Culture, CMV ..............................................................................................................735
Culture, CSF for AFB see Culture, Mycobacteria, CSF .............................................736
Culture, CSF & Gram Stain ........................................................................................737
Culture, Cytomegalovirus see Culture, CMV .............................................................738
Culture, Ear .................................................................................................................739
Culture, Eye.................................................................................................................740
Culture, Fungus ...........................................................................................................741
Culture, Fungus, Bone Marrow ...................................................................................742
Culture, Fungus, Skin ..................................................................................................743
Culture, Genital Sites ..................................................................................................744
Culture, Legionella ......................................................................................................745
Culture, Mycobacteria .................................................................................................746
Culture, Mycobacteria, Blood .....................................................................................747
Culture, Mycobacteria, Bone Marrow ........................................................................748
Culture, Mycobacteria, CSF ........................................................................................749
Culture, Mycoplasma Pnemoniae ...............................................................................750
Culture, Mycoplasma/Ureaplasma ..............................................................................751
Culture, Nose...............................................................................................................752
Culture, Pertussis.........................................................................................................753
Culture, Skin ...............................................................................................................754
Culture, Sputum and Gram Stain ................................................................................755
Culture, Stool ..............................................................................................................756
Culture, Throat (Comprehensive) ...............................................................................757
Culture, Throat Beta Strep Group A ...........................................................................758
Culture, Throat, for C. Diphtheria ...............................................................................759
Culture, Throat, for Neisseria Gonorrhoeae ................................................................760
Culture, Tissue ............................................................................................................761
Culture, Urine, Catheterized .......................................................................................762
Culture, Urine Midvoid Specimen ..............................................................................763
Culture, Urine, Suprapubic Puncture ..........................................................................764
Culture, Wound, Miscellaneous Sites .........................................................................765
Cyclospora Stain .........................................................................................................766
PCR for Neisseria Gonorrhoeae .................................................................................767
Enterovirus PCR (CSF) ...............................................................................................768
Fetal Fibronectin Test .................................................................................................769
Fungal Wet Prep see KOH Prep..................................................................................770
GBS Screen see Group B Strep Screen .......................................................................771
G.C. Culture, Throat see Culture, Throat, for Neisseria Gonorrhoeae .......................772
GC DNA Probe see DNA Probe for Neisseria Gonorrhoeae ......................................773
Giardia Antigen ...........................................................................................................774
Gram Stain ..................................................................................................................775
Group B Strep Screen (Culture/PCR) .........................................................................776
India Ink Prep ..............................................................................................................777
Influenza PCR .............................................................................................................778
KOH Prep ....................................................................................................................779
Legionella Culture see Culture, Legionella ................................................................780
Legionella DFA ...........................................................................................................781
Legionella Urine Antigen ............................................................................................782
MAV Blood Culture see Culture, Mycobacteria, Blood .............................................783
Maximum Bactericidal Dilution (MBD) .....................................................................784
MBC see Minimum Bactericidal Concentration .........................................................785
MBD see Maximum Bactericidal Dilution (MBD) ....................................................786
MIC see Minimum Inhibitory Concentration (Send Out Test) ...................................787
Minimum Bactericidal Concentration .........................................................................788
Minimum Inhibitory Concentration (Special request Send Out Test) ........................789
MRSA Screen PCR .....................................................................................................790
MRSA/SA Screen PCR (soft tissue) ..........................................................................791
O& P, Stool for Amoeba, Worm Identification see Ova and Parasites ......................792
Occult Blood, Guaiac Method.....................................................................................793
Pinworm Exam ............................................................................................................794
Pinworm Prep see Pinworm Exam ..............................................................................795
Pneumocystis DFA......................................................................................................796
Respiratory Syncytial Virus ........................................................................................797
Rotavirus Exam ...........................................................................................................798
RSV Exam see Respiratory Syncytial Virus ...............................................................799
Schlicter Test see Maximum Bactericidal Dilution (MBD)........................................800
Scotch tape prep see Pinworm Exam ..........................................................................801
Semen Analysis ...........................................................................................................802
Serum Cidal Test see Maximum Bactericidal Dilution (MBD) ..................................803
Skin scrapings for fungus see Culture, Fungus, Skin..................................................804
Sperm Post Vasectomy ...............................................................................................805
Sputum for PCP see Pneumocystis DFA ....................................................................806
Staph aureus/MRSA Nasal Screen PCR .....................................................................807
Stool for Blood see Occult Blood, Guaiac Method .....................................................808
Stool for Enteric Pathogens see Culture, Stool ...........................................................809
Stool for Guaiac see Occult Blood, Guaiac Method ...................................................810
Trichomonas Rapid Test .............................................................................................811
Urine for G.C. see Culture, Urine for Neisseria Gonorrhoeae ....................................812
VRE PCR ....................................................................................................................813
Wet prep for Trichomonas see Trichomonas Prep ......................................................814
Whooping Cough see Culture, Pertussis .....................................................................815
URINALYSIS ............................................................................................ 816
Clinitest .......................................................................................................................817
Eosinophils in Urine ....................................................................................................818
EOUR see Eosinophils in Urine ..................................................................................819
Hemosiderin, Urine .....................................................................................................820
Routine Urine see Urinalysis - Routine ......................................................................821
Urinalysis – Routine ....................................................................................................822
Urine Bilirubin ............................................................................................................823
Urine Blood .................................................................................................................824
Urine Glucose..............................................................................................................825
Urine Iron see Hemosiderin, Urine .............................................................................826
Urine Ketones..............................................................................................................827
Urine Leukocyte Esterase ...........................................................................................828
Urine Nitrites ...............................................................................................................829
Urine pH ......................................................................................................................830
Urine Protein ...............................................................................................................831
Urine Specific Gravity ................................................................................................832
Urine Urobilinogen .....................................................................................................833
PREFACE
This manual is dedicated to continued excellence in service by the laboratory staff of the
Lawrence + Memorial Hospital. It is designed to improve communication between
laboratory personnel and staff physicians, nurses, health unit coordinators and medical
assistants who utilize the services of the laboratory.
An attempt was made to list pertinent details regarding each test: the type of specimen
required; section performing test; turn-around time and reference values. The availability
of each test is also indicated under the specific test listing.
The test listings are arranged alphabetically by department and alphabetically in the index
of this manual. A STAT LIST is included for quick reference (See pages 25- 26).
For the purposes of this manual, the department is divided into nine sections including
Anatomic Pathology, Blood Bank, Reference Lab, Chemistry, Coagulation, Cytology,
Hematology, Microbiology, and Urinalysis. Support is supplied by the Information
Services, Phlebotomy, Secretarial, Central Receiving Center and Call Center sections.
The laboratory is inspected and approved by the College of American Pathologists, Joint
Commission, and CLIA. Extensive quality control programs include proficiency surveys
of the American Society of Clinical Pathologists and College of American Pathologists.
A repeat laboratory test will be performed at no extra charge whenever a physician has
any question as to the validity of a test result. Staff consultation will be available if
needed to resolve such questions.
Pathologists:
Victoria G. Reyes-D'Arcy, M.D., D.A.B.
Director of Laboratories
Joseph C. Benedict, M.D., D.A.B.
Associate Pathologist
Nicole E. Muscato, M.D., D.A.B.
Associate Pathologist
Kevin B. Green, M.D., D.A.B.
Associate Pathologist
Anica Antic, M.D., D.A.B.
Associate Pathologist
Asim Ejaz, M.D., D.A.B.
Associate Pathologist
Elise L. Krejci, M.D., D.A.B.
Associate Pathologist
Pathologist Assistant:
Michelle Zaremba, MHS, PA (ASCP)
Administrative Director:
Nicole Gomes, BS, MT (ASCP)
Administrative Director of Laboratory
Administrative Consultant:
Judith Portelance, MS, BS, DLM (ASCP)
Administrative Consultant
Section Managers:
Joan Blessing, BA - Pathology Office Manager
Kimberly Brown, MBA , BS, HTL (ASCP) –Histology
Darleen Chretien, BS, MT (ASCP) - Pequot Laboratory
Elizabeth DeRosa, BS, MT (ASCP) SBB - Blood Bank
Sarah Glennon, MAT, BS M, MLT (ASCP) – Phlebotomy
Melissa Grandolfi, MS, CT (ASCP) - Cytology
Barbara Naillis, BS, MT (ASCP) - Chemistry/Serology/Point of Care Testing
Reference Lab
Peter Speciale, MHS, PHD, M (ASCP) sm – Microbiology
Audrey Turner, AS, PBT (ASCP) – Outreach/Call Center/Central Receiving Center
Pedro Ugarelli, MS, BS, MT (ASCP) - Coagulation/Hematology/Urinalysis,
Cancer Center Laboratory
Laboratory Main Number: x5101
Test Priorities
The following test priorities are available:
ROUTINE: These specimens are collected beginning at 4:00 AM the morning after
receiving the order.
STAT: Includes selected tests (see STAT List Page 26), which can be ordered 24 hours
per day, 7 days per week. These tests will be collected and processed immediately.
(Average turn-around time: one (1) hour after receipt).
URGENT: These tests can be ordered any time with telephone notice and followed by an
order. It should be noted that collection of the specimen is immediate. The specimen will
be processed with the next regularly scheduled run.
Inpatients vs Outpatients
Inpatients
Tests for inpatients are available as ROUTINE, STAT, and URGENT.
Outpatients
Tests for outpatients are handled as ROUTINE whenever possible. In certain
circumstances (arrival of specimen after last regularly scheduled run or specialized test
which is not run on a daily basis), test requests may receive STAT handling. STAT tests
are available for emergency department patients.
Outpatients requiring laboratory services are registered by the outpatient registration
staff. They are then directed to the outpatient laboratory where the specimen(s) is
obtained. Outpatient laboratory areas and hours of operation include:
ACC Building: Monday through Friday 6:30 AM to 6:00 PM, Saturday 7:00 AM to 12
noon
Pequot Treatment Center: Monday through Sunday 6:30 AM to 7:00 PM
Flanders Health Center: Monday through Friday 6:30 AM to 5:00 PM; Saturday 7:00
AM to 12 Noon.
Shaw's Cove Patient Facility: Monday through Friday 8:30 AM to 5:00 PM.
Home Blood Drawing
As a convenience for our homebound patients, we offer a home blood drawing service.
To provide this service we must receive a L&M HOME DRAW REQUEST FORM that
has been filled out completely, signed and dated by the ordering physican specifying the
date of service to start as well as the frequency of draw for the ordered test(s). We prefer
at least one day's notice for all home draw requests.
To request a blank home draw requisition, please call 860-444-5101.
STAT Laboratory Work
Only the following tests are available as STAT procedures. Others can occasionally be
performed by arrangement with the Pathologist on call.
HEMATOLOGY:
CBC
Platelet Count
Hemoglobin (Hgb)
Hematocrit (Hct)
White Blood Count (WBC)
Spinal Fluid Counts (CSFCT)
IMMUNOLOGY/SEROLOGY:
Pregnancy test
Monospot
BhCg. QuantEstradiol and Progesterone Mon-Fri 7-11 PM
Sat & Sun 7-3 PM
Amniotic Fluid - PG Slide test only.
*L/S Ratio must be approved by Pathologist on call
COAGULATION:
Fibrinogen Level
Prothrombin Time
INR
Activated Partial Thromboplastin Time (APTT)
D-Dimer
BLOOD BANK:
Emergency release of group O packed cells
ABO/Rh
Antibody screen
Transfusion Reaction Workup
Crossmatch
Direct Antiglobulin Test
CHEMISTRY:
Acetone
Alcohol (Medical reasons only; not MEDICAL LEGAL)
Amylase
Bilirubin
BUN
Calcium
Creatinine
Lithium
Drugs of Abuse Screen
Electrolytes (Na, K, Cl, CO2)
Therapeutic Drugs
Enzymes (Alk Phos, CPK, LDH, SGOT, SGPT)
Glucose
Ionized Calcium
Lactic Acid
Salicylate
Spinal Fluid protein and sugar
TP-A/G
Uric Acid
Lithium
Theophylline
Therapeutic Drugs
Heart Profile (Troponin & CPK-MB)
Carboxyhemoglobin
Methemoglobin
MICROBIOLOGY:
Blood Culture Drawn
Spinal Fluid Culture Planted
Gram Stain of spinal fluid or sputum
FFN (Fetal Fibronectin)
Rapid (10 minute) Beta Strep Screen
URINALYSIS:
Complete urinalysis
Any culture after 11:00 PM considered 'STAT' by the attending M.D. can be processed
on request.
Routine cultures received after 11:00 PM are placed in the night specimen refrigerator
and processed the next morning.
Laboratory Critical Results (Panic Values):
The following is a list of those life-threatening values, which must be telephoned to the
physician or representative:
White Blood Count
Absolute neutrophils
Blasts
Babesia, Malaria, Ehrlichia
Hemoglobin
Hematocrit
Platelet Count
INR
PTT
Fibrinogen
Bilirubin, newborn (0-30 d)
Bilirubin, cord blood
Serum Calcium
Glucose
Potassium
Sodium
CO2
Creatinine
Troponin
Blood Culture
CSF Gram Stain and Culture
Isolation of Presumed E coli 0157
Culture
Gram Stain, Sterile Body Site
AFB Smear, Inpatient
Direct Coombs, Cord Blood
Arterial/Venous Blood Gas
Arterial/Venous Blood Gas
Arterial Blood Gas
Acetaminophen
Digoxin
Dilantin (Phenytoin)
Gentamicin
Lithium
Phenobarbital
Salicylate
Tegretol (Carbamazepine)
Theophylline
Tobramycin
Valproic Acid
Vancomycin
≤ 2,000 OR ≥ 35,000 white cells/dl
≤ 1,000 neutrophils/dl
Present
Present
≤ 7.0 gm/dl
≤ 20%
Newborn (0-60 days) ≤ 30% OR ≥ 65%
Child (2 months to 6 years) ≤25%
≤ 50,000 platelets/dl
≥ 5.0
≥ 100 seconds
Newborn (0-30 days) ≥ 60 seconds
> 1000 mg/dl
> 15 mg/dl
> 4 mg/dl
≤ 6.5 mg/dl OR ≥ 13 mg/dl
≤ 50 mg/dl OR ≥ 400 mg/dl
Newborn (0-30 days) ≤ 40 mg/dl OR ≥ 200 mg/dl
≤ 3.0 mmol/L OR ≥ 6.0 mmol/L
Newborn (0-30 days) ≤ 3.0 mmol/L OR ≥ 6.5 mmol/L
≤ 120 mmol/L OR ≥ 155 mmol/L (adult: 17 yrs or older)
Newborn/child (0 days – 16 yrs) ≤ 125 mmol/L OR ≥ 155
mmol/L
≤ 10 mmol/L
≥7.0 mg/dl
Newborn (0-30 days) >1.5 mg/dl
≥1.00 ng/ml
Positive
Positive
In stool
Positive for organism associated with bioterrorism
Positive
Positive
Positive
PCO2 >50 mm Hg and pH <7.30
PCO2 <30 mm Hg and pH >7.50
PO2<55 mm Hg
>150 ug/ml
>2.0 ng/ml
>30 ug/ml
>10 ug/ml
>1.5 mmol/L
>50 ug/ml
>30 mg/dl
>15 ug/ml
>20 ug/ml
>10 ug/ml
>120 ug/ml
>60 ug/ml
L+M Cancer Center Critical Results (Panic Values):
The following is a set of results explicitly for use with lab results originating within the
Cancer Center. For those labs, items on Cancer Center-specific list of critical laboratory
results supersede the general critical results list. If the result is not on the Cancer-Center
specific list, then the parameters on the general list stand.
White Blood Count
Blasts
Hemoglobin
Hematocrit
Platelet Count
INR
Serum Calcium
≤ 500 OR ≥ 100,000 white cells/dl
Forward only to pathologist for review. Do not call oncologist.
≤ 7.0 gm/dl
≤ 20% OR ≥55%
≤ 10,000 OR ≥ 1,000,000 platelets/dl
≥ 5.0
≤ 6.0 OR ≥ 12.0 mg/dl
Chloride
≤ 70 mmol/L OR ≥ 140 mmol/L
CO2
≤ 15 mmol/L OR ≥ 40 mmol/L
Creatinine
Glucose
Magnesium
Potassium
Sodium
AST
ALT
Uric Acid
≥2.0 mg/dl (If patient < 2.0 mg/dL previously)
≤ 50 mg/dL OR ≥ 350 mg/dl
≤ 1.1 mg/dL OR ≥ 3.6 mg/dl
≤ 3.0 mmol/L OR ≥ 5.5 mmol/L
≤ 125 mmol/L OR ≥ 155 mmol/L
≥ 500 U/L
≥ 500 U/L
≥ 12.0 mg/dl
Critical results which indicate a possible life-threatening situation (i.e., Panic Values) are
called to the floor or ordering/covering/on-call physician within 60 minutes after a critical
result is obtained.
The message is not given to clerical personnel in-house. Individuals may be designated
by the physician to receive such critical results in the outpatient setting.
The first and last name of the physician/office designee or nurse notified is recorded on
the patient report.
After hours and on weekends, critical values are also faxed to the outpatient office.
Please refer to the policy Critical Tests and Critical Values in the L+M Hospital Policy
Manual.
ANATOMIC PATHOLOGY:
The Pathology Department is chaired by Victoria Reyes MD. Histology and Autopsy are under
the immediate direction of Kevin Green MD. The Dept is staffed at all times with AP/CP board
certified pathologists. The Pathologist Assistant (PA) has been formally trained in laboratory and
gross room safety, specimen accessioning in CoPath, and all grossing and tissue processing
aspects, including touch preparations, frozen sections, and handling of different tissue specimens.
All tissue removed in Lawrence + Memorial Hospital's Main Campus or satellite facilities must
be processed by a pathologist or PA, and examined by a pathologist at the gross and/or
microscopic level. Exempt specimens are approved by the Medical Executive Committee
although ultimate discretion is left to the attending surgeon.
Surgical Pathology reports on routine biopsy specimens are available within 48 hours following
accession of the specimen. Slides for microscopic evaluation of routine cases are generally not
available prior to 11:00 AM. Every effort will be made to expedite cases where early discharge of
the patient is contingent upon the Surgical Pathology report. Large specimens requiring
prolonged fixation, decalcification, ancillary testing, extra-departmental consultation, and sentinel
lymph node cases may delay the Surgical Pathology report. Tissue is held for 2 weeks after the
case is signed out. Slides and blocks are held for 10 years.
The Pathology Dept. is located on the third floor of the Hospital on 3.3, and is open M-F for
receipt of routine specimens from 8:00 AM until 4:00 PM and 8:00 AM to 12:00 noon on
Saturday. An on-call pathologist is available 24/7/52.
Slides for Outside Consultation:
If a patient at Lawrence + Memorial Hospital is referred to an outside institution or if a clinician
desires a second opinion from an outside institution, the Pathology Dept. must be notified such
that slides and a copy of the surgical pathology report are forwarded to the outside institution in a
timely manner. Ideally, twenty-four hours advance notice is given for such requests. Clinicians
may also send outside slides and corresponding reports to the Pathology Department for review
and documentation at L+M Hospital.
Note that patients may not hand carry slides given risk of breakage or loss in transit. Slides and/or
paraffin blocks sent in consultation to referral institutions are tracked by internal notation in
CoPath.
Critical Results:
Defined critical results are relayed to the clinician in a timely manner. Every attempt will be
made to record date and time of notification in the permanent record. Critical results include all
new and/or unexpected malignancies (except skin), significant and unexpected findings,
vasculitides, the presence of fat in endometrial or endoscopic biopsies, absence of chorionic villi
in POC, and invasive fungal infections, among others. Critical results are reviewed from time to
time by the Dept. Any changes are made at the level of Dept. Chair after discussion and
notification within the Dept.
Specimen Labeling:
In accordance with the Lab’s accreditation agencies (CAP, JC) and its internal Specimen
Labeling and Irreplaceable Specimen policy (available on the hospital intranet under Lab), all
submitted specimens must be accompanied by a properly completed specimen label and Surgical
Pathology Request Form.
Specimen Container/Label:
The specimen label must include at two patient identifiers (patient’s full name and date of
birth).
The specimen label must include the specimen source.
Appropriate containers with fixative may be obtained from Surgical Pathology (x 5100)
Surgical Pathology Request Form (Requisition):
The Surgical Pathology Request Form must be completed by the physician /LIP or
designee, and must include (but is not limited to): patient’s full name, date of birth, specimen
source, ordering physician information, all relevant history and/or clinical impression, and any
special orders/additional information (eg, orientation) to facilitate appropriate triage and
diagnosis.
Un/Mislabeled Specimens:
Because an unlabeled or mislabeled specimen usually cannot be recollected, an
Irreplaceable Specimen Form (ISF) will be sent to the MD/LIP for completion in the outpatient
setting. If such a mislabeled or unlabeled specimen is received in-house (e.g., operating room,
endoscopy suite, patient floor), it may be identified in Histology by a designated person. All
events are documented and presented at Lab Quality Committee. Quality Referrals may be
submitted, if appropriate..
Specimen Submission/Handling:
Surgical specimens submitted from in-house patients are first ordered within the corresponding
HIS under Pathology > Histology. Those specimens submitted from private offices do not enter
the HIS. All specimens received should be accompanied by a paper Surgical Pathology Request
Form (Requisition) that is retained on-site for a minimum of two years. Appropriate containers
with fixative/preservative may be obtained from Surgical Pathology (x 5100).
Routine Specimens:
Test Availability: M-F 8a-4p; Sat. 8a-12p
Prior Notification to Pathology: No
Meditech Exam Requested: Routine/Permanent
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Formalin, Fresh/Saline (refrigerated)
Specimen Submission:
Routine Specimens are submitted in formalin for standard histology processing. Results
are reported by a pathologist within 48 hours of receipt. Specimen may also be submitted
fresh to the Histology Department with subsequent fixation in formalin. If specimens are
submitted fresh or in saline (especially after hours), then every effort must be made to
keep the specimens refrigerated and delivered to the Laboratory as soon as possible to
preserve tissue integrity and decrease risk of compromised processing and interpretation.
Fresh Specimens:
Test Availability: M-F 8a-4p; Sat. 8a-12p
Prior Notification to Pathology: No
Meditech Exam Requested: Fresh
Paperwork Required: Surgical Pathology Request Form (indicating FRESH specimen)
Specimen Handling: Fresh, Immediate delivery
Specimen Submission:
Many ancillary tests (see Special Test Request/Submission section below) require fresh
tissue specimens. These special tests cannot be performed on formalin fixed tissue.
Therefore, clinicians may submit specimens fresh or in minimal saline (to keep the tissue
moist) to ensure all potential appropriate testing may be performed. In such cases,
specimens must be delivered immediately to the Laboratory and labeled “FRESH” for
appropriate triage and processing, and to avoid compromise of specimen integrity. If the
specimen is obtained after hours, specimens must be kept refrigerated. Consult a
pathologist if questions arise.
Intra-Operative Consultation Specimens:
Test Availability: 24 hours
Prior Notification to Pathology: Yes
Meditech Exam Requested: Intra-Operative Consultation
Paperwork Required: Surgical Pathology Request Form
Intra-Operative Consultation Form
Specimen Handling: Fresh, Immediate delivery
Specimen Submission:
Intra-Operative Consultation Specimens require immediate gross and/or microsopic
evaluation by a pathologist during an operative procedure. Surgeons generally request
intra-operative consultations to direct the immediate next step in surgery or to inform
treatment. These specimens must be sent fresh to the Histology Department immediately
for triage and interpretation, along with the Surgical Pathology Request Form and the
Intra-Operative Consultation Form.
The PA/Pathologist will perform either a Gross Consult, Touch Preparation, or Frozen
Section on the submitted tissue. The Pathologist will render an interpretation and verbally
communicate his/her findings to the requesting surgeon by phone or intercom. If
additional fresh tissue is needed, then the pathologist will inform the submitting
physician. Discussion between the pathologist and surgeon may occur during intraoperative consultations to obtain additional information (eg, other intra-op findings,
diagnostic imaging results, previous patient history).Rarely the requesting physician may
not be available to receive the result. In this situation, the pathologist will make every
effort to speak directly with the surgeon and verbally communicate the intraoperative
consultation findings.
After the Intra-Operative Consult is performed, it is recorded on the Intra-Operative
Consultation Form with the Pathologist's signed diagnosis. This Intra-Op Consult Form
becomes part of the patient's record and is placed in the paper medical chart.
If multiple specimens are sent for Intra-Operative Consult, EACH specimen must be
accompanied by a complete label, Surgical Pathology Request Form, and Intra-Operative
Consultation Form.
Pathologists are made aware of scheduled intra-operative consults the day before the
operation or when the O.R. schedule is delivered to the Laboratory.
*If a non-scheduled consult is requested during an operation occurring between 8:00 AM
and 5:00 PM, notify the Path Dept. at x5100 before sending tissue to Histology.
*If an Intra-Operative Consultation is required on Sunday, a holiday, after 12:00 Noon on
Saturday or after 5:00 PM on a weekday, the attending surgeon must contact the
pathologist on-call by telephone to discuss, in advance, the pertinent clinical information
and concerns to be addressed by the consult (margins, presence of tumor, specimen
adequacy, etc). Stand-by or add-on cases that require intra-operative consult must be
communicated to the Pathology office (x5100).
Lymph Node Exam Specimens:
Test Availability: 24 hours
Prior Notification to Pathology: Yes
Meditech Exam Requested: Lymph Node Exam
Paperwork Required: Surgical Pathology Request Form
Microbiology Orders
Lymph Node Exam Form
Specimen Handling: Fresh, Immediate delivery
Specimen Submission:
A Lymph Node Exam is typically reserved for cases to R/O Lymphoma and/or R/O
Infection. Specimens are immediately sent sterile and fresh to Histology with a Surgical
Pathology Request Form, a Lymph Node Exam Form, and Microbiology Orders. The
specimen is triaged immediately in the fresh state and samples taken for all appropriate
testing, at the discretion of the attending Pathologist, who may or may not consult with
the surgical attending.
Bone Marrow Specimens:
Bone Marrow specimens involve multiple specimens that are handled in Hematology,
Histology, and Pathology. For a step-by-step complete guide, please reference the Hematology
Section: Bone Marrow. Consult a Pathologist with any questions.
Special Test Request/Submission:
Special, often ancillary and/or esoteric, tests may be requested by the submitting clinician and/or
Pathologist. These tests may require specific handling, submission, preparation and additional
paperwork. Any questions regarding special testing should be directed to the Pathology
Department (x5100) prior to specimen collection to ensure appropriate and adequate tissue is
obtained and submitted.
Muscle Biopsy:
Test Availability: M-Th 8a-12p
Prior Notification to Pathology: Yes, 24 hours
Meditech Exam Requested: Fresh
Paperwork Required: Surgical Pathology Request Form
Reference Lab Requisition
Specimen Handling: Fresh, Immediate delivery
Specimen Submission:
The test is available M-Th prior to 12:00 Noon.
Surgical Pathology (x5100) is to be notified 24 hours in advance and all appropriate
paperwork completed (Reference Lab Requisition).
Three portions of skeletal muscle will be obtained and submitted in two Price clamps.
Specimens with attached Price clamps must be delivered immediately by the OR staff on
saline-moistened gauze to Histology with a Surgical Pathology Request Form.
The lab will process the specimen and properly package/ship the specimen to the
reference laboratory according to protocol. Clamps will be returned to the O.R
Cytogenetics/Chromosome Analysis/Karyotyping:
Test Availability: M-F 8a-3p
Prior Notification to Pathology: Yes
Meditech Exam Requested: NA
Paperwork Required: Reference Lab Cytogenetics Requisition
Specimen Handling: Fresh/RPMI
Specimen Submission:
All cytogenetic testing (chromosome analysis, karyotyping, etc.) must be done on fresh
tissue submitted in RPMI fluid.
DO NOT PLACE IN FIXATIVE!
Test orders are not placed in the HIS.
For efficient testing, samples are to be submitted to Histology M-F, prior to 3pm.
If a clinician plans to obtain the tissue sample:
- Obtain necessary RPMI fluid and a Cytogenetics Requisition (for the reference lab)
from Histology, prior to the scheduled procedure.
- Place tissue sample (2-3mm pieces) in RPMI.
- Return RPMI sample and requisition to Histology as soon as possible. If immediate
submission is not feasible, keep RPMI refrigerated.
- If an additional tissue sample is desired for routine histology, submit the specimen
separately according to the Routine Specimen Submission/Handling guidelines (above)
For Lab/Histology triage and submission:
- Send a fresh specimen with the Surgical Pathology Request Form to Histology
immediately, indicating specimen is for cytogenetics.
Flow Cytometry (R/O Lymphoma):
Test Availability: M-F 8a-3p
Prior Notification to Pathology: Yes
Meditech Exam Requested: NA
Paperwork Required: Reference Lab Flow Cytometry Requisition
Specimen Handling: Fresh/RPMI
Specimen Submission:
All flow cytometry testing (testing for lymphoma) must be done on fresh tissue submitted
in RPMI fluid.
DO NOT PLACE IN FIXATIVE!
Test orders are not placed in the HIS.
For efficient testing, samples are to be submitted to Histology M-F, prior to 3pm.
If a clinician plans to obtain the tissue sample:
- Obtain necessary RPMI fluid from Histology, prior to the scheduled procedure.
- Place tissue sample (2-3mm pieces) in RPMI.
- Return RPMI sample to Histology during working hours (M-F, 8-3pm), as soon as
possible. If immediate submission is not feasible, keep RPMI refrigerated.
**NOTE: A Pathologist will complete the Reference Lab Requisition; therefore
the specimen must always be delivered directly to Histology staff to ensure proper
communication of testing!
- If an additional tissue sample is desired for routine histology, submit the specimen
separately according to the Routine Specimen Submission/Handling guidelines (above)
For Lab/Histology triage and submission:
- Send a fresh specimen with the Surgical Pathology Request Form to Histology
immediately, indicating specimen is for flow cytometry.
Histology + Microbiology (Shared Specimen):
Test Availability: 24 hours
Prior Notification to Pathology: No
Meditech Exam Requested: Microbiology (as desired); Histology > Fresh
Paperwork Required: Surgical Pathology Request Form
Microbiology Orders
Specimen Handling: Fresh, Immediate delivery
Specimen Submission:
Separate and distinct specimens for histology and microbiology are preferred for lab
submission.
In the event only a single specimen is obtained and a clinician would like both histology
and microbiology performed:
- The specimen must be submitted sterile/fresh with a pink "Shared Specimen" sticker.
- Histology and Microbiology orders and requisitions are completed and delivered with
the one specimen.
- Specimen and paperwork are submitted to the following department for triage
M-F 7a-5p = Deliver to Histology
M-F 5p-11p = Deliver to Central Receiving Center (CRC)
M-F 11p-7a = Deliver to Microbiology
Saturday 7a-12p = Deliver to Histology
Saturday 12p-11p = Deliver to Central Receiving Center (CRC)
Saturday 11p-7a = Deliver to Microbiology
Sunday 7a-11p = Deliver to Central Receiving Center (CRC)
Sunday 11p-7a = Deliver to Microbiology
Foreign Bodies/Implants (Gross Only):
Test Availability: M-F 8a-4p
Prior Notification to Pathology: No
Meditech Exam Requested: Routine/Permanent; Fresh
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Fresh; Formalin
Specimen Submission:
Foreign bodies, etc. are handled as tissue specimens and sent to the lab. If the surgeon
wants a specimen, he/she must sign a release form AFTER it is described (and preferably
signed out) by the pathologist.
Some specimens (e.g. teeth, gallstones) are given gross descriptions only as no tissue is
available to process.
Stone Analysis(Gross Only):
Test Availability: M-F 8a-4p
Prior Notification to Pathology: No
Meditech Exam Requested: Routine/Permanent; Fresh
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Fresh; Formalin
Specimen Submission:
Stones can be handled as tissue specimens and sent to the lab. If the surgeon wants a
specimen, he/she must sign a release form AFTER it is described (and preferably signed
out) by the pathologist.
In the event component analysis is desired, stones can be sent to Specimen Processing.
For detailed submission instructions, see CHEMISTRY: Stone Analysis
Kidney Biopsy (Medical):
Test Availability: M-F 8a-3p
Prior Notification to Pathology: Yes
Meditech Exam Requested: Routine; Fresh
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Fresh in labeled/oriented Petri Dish, Immediate delivery
Specimen Submission:
All non-neoplastic kidney biopsy testing must be done on fresh tissue.
DO NOT PLACE IN FIXATIVE!
Testing includes light, electron, and immunofluorescence microscopy
For efficient testing, samples are to be submitted to Histology M-F, prior to 3pm., with a
24 hour notification.
- Send tissue fresh on normal saline-moistened Telfa gauze in a sealed, labeled Petri dish.
- Identify the cortical and medullary ends of the biopsies (preferably on the specimen
label sticker).
- Deliver to the Histology Lab immediately, with the Surgical Pathology Request Form,
and notify a Histotechnologist, PA or a Pathologist.
** DO NOT leave the specimen without notifying Histology/Lab staff**
Direct Immunofluorescence (IF):
Test Availability: M-F 8a-3p
Prior Notification to Pathology: Yes
Meditech Exam Requested: Routine; Fresh
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Fresh; Michel's Transport Media
Specimen Submission:
All immunofluorescence testing for antibodies/autoimmune diseases, must be done on
fresh tissue placed in Michel's Transport Media.
DO NOT PLACE IN FIXATIVE!
For efficient testing, samples are to be submitted to Histology M-F, prior to 3pm., with a
24 hour notification.
If a clinician plans to obtain the tissue sample:
- Obtain necessary Michel's Transport Media fluid from Histology, prior to the scheduled
procedure.
- Place tissue sample in Michel's Transport Media.
- Return sample to Histology during working hours (M-F, 8-3pm) with Surgical
Pathology Request Form, indicating immunofluorescence testing (IF) testing is requested.
- If an additional tissue sample is desired for routine histology, submit the specimen
separately according to the Routine Specimen Submission/Handling guidelines (above)
For Lab/Histology triage and submission:
- Send a fresh specimen with the Surgical Pathology Request Form to Histology
immediately, indicating specimen is for immunofluorescence testing (IF).
**Indicate if entire specimen is for IF, or to be split for both IF and routine histology**
Quantitative Iron/Copper:
Test Availability: M-F 8a-3p
Prior Notification to Pathology: No
Meditech Exam Requested: Routine/Permanent
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Formalin
Specimen Submission:
Liver biopsies can be submitted for Iron/Copper testing in the same manner as Routine
Specimens.
An indication should be made on the Surgical Pathology Request Form of either Iron or
Copper test request.
Transmission Electron Microscopy:
Test Availability: M-F 8a-3p
Prior Notification to Pathology: Yes
Meditech Exam Requested: Routine; Fresh
Paperwork Required: Surgical Pathology Request Form
Specimen Handling: Fresh; Glutaraldehyde
Specimen Submission:
All transmission electron microscopy (TEM; EM) testing for cellular ultra-structure must
be done on glutaraldehyde fixed tissue.
DO NOT PLACE IN FORMALIN!
For efficient testing, samples are to be submitted to Histology M-F, prior to 3pm., with a
24 hour notification.
If a clinician plans to obtain the tissue sample:
- Obtain necessary glutaraldehyde fixative from Histology, prior to the scheduled
procedure.
- Place tissue sample in glutaraldehyde fixative.
- Return sample to Histology during working hours (M-F, 8-3pm) with Surgical
Pathology Request Form indicating specimen is for transmission electron microscopy
(TEM; EM).
- If an additional tissue sample is desired for routine histology, submit the specimen
separately according to the Routine Specimen Submission/Handling guidelines (above)
For Lab/Histology triage and submission:
- Send a fresh specimen with the Surgical Pathology Request Form to Histology
immediately, indicating specimen is for transmission electron microscopy (TEM; EM).
**Indicate if entire specimen is for EM, or to be split for both EM and routine
histology**
Autopsy (Adult):
The autopsy suite is located on the second floor of the 100 building room #2.120B.
The Medical Staff is encouraged to obtain a post-mortem examination in all cases of unusual
deaths and of medicolegal and educational interest.
The Post-mortem Examination Permission and Consultation Request, which can be found on the
hospital intranet (i.e., autopsy permit), is obtained through the efforts of the attending physicians
in cooperation with the Admitting Coordinator. The full policy/procedure for requesting an adult
autopsy is located in Patient Care Services.
When a valid permit for autopsy is obtained, the Laboratory is notified. The autopsy permit must
be completed by the person assuming responsibility for burial of the deceased and witnessed by
the attending physician or other individual obtaining permission. Under no circumstances should
a funeral director be notified to remove a body on whom an autopsy permit has been obtained
prior to release by the laboratory.
Please note all autopsies are performed by a pathologist with the assistance of a Diener Service
and/or of the Pathologists' Assistant (PA). Private post mortem examinations are available upon
request and incur a charge for pathology services and hospital facilities.
An autopsy will not be performed until the pathologist has, in-hand, a properly signed
authorization. A valid permit is defined in Section 10-143 of the Connecticut State Public Health
Codes:
Section 19-143
Section 19a-286 (formerly Sec. 19-143). Autopsies, consent for. Performance or attendance by
nonaffiliated physician. (a) Whenever any person dies and no postmortem examination or
autopsy has been ordered pursuant to subsection (b) of section 19a-406, no physician shall
conduct or assist in conducting any postmortem examination or autopsy upon the body of such
deceased person without first obtaining the consent of whichever one of the following persons,
eighteen years of age or older, assumes custody of the body for the purposes of burial; Father,
mother, husband, wife, child, guardian, next of kin, friend or any person charged by law with the
responsibility for burial. If two or more persons assume custody of the body, consent of one of
them shall be deemed sufficient. Any such consent may be in writing or may be given by telephone
provided a record of any such consent by telephone shall be kept by such physician for not less
than three years.
An autopsy may be performed at another facility or attended by a physician who is not affiliated
with Lawrence + Memorial, but any resultant fees or expenses (including but not limited to costs
of transfer of the deceased to another facility) will be borne by the person requesting those
actions.
Permission for autopsy may be obtained by telephone. The permission must be overheard by two
witnesses to the conversation.
When the family wishes to limit the extent of the examination, the restriction must be recorded on
the autopsy permit form.
In possible medicolegal cases, the Medical Examiner (ME) must be contacted at (860) 679-3980
before any suggestion regarding autopsy permission is made to the family of the deceased.
Indications for Medical Examiner input include, but are not limited to:
- Suspected toxin
- Sudden or unexpected death
- Death due to disease which might constitute a threat to the public health
- Death at or related to the workplace
- Death occurring under suspicious circumstances (e.g., child abuse)
Note: ME Autopsies are performed on all homicide victims and gunshot victims. In
addition, the vast majority of pedestrian deaths, Sudden Death syndrome, overdoses,
industrial accidents, sudden and otherwise unexplained deaths under the age of 45, and a
variety of other types of cases are subject to autopsy examination.
Autopsies not falling under the jurisdiction of a Medical Examiner are limited to the bodies of
individuals whose death is pronounced while in Lawrence + Memorial Hospital as an inpatient.
These autopsies are performed as a service without charge to the family of the deceased.
Arrangements for an autopsy on the body of deceased individuals not admitted to Lawrence +
Memorial Hospital, including deaths occurring in the Emergency Department, may be made by
the family on a fee-for-service basis, with the hospital and pathologist.
Clinicians and pathologist may discuss the case before autopsy is performed to clarify any
outstanding clinical concerns or questions. Clinicians are notified when the autopsy will be
performed. Final anatomic diagnoses on post-mortem examinations will be sent to the attending
of record for inpatient autopsy cases.
Post-mortem examinations are performed in the hospital in the autopsy suite. Attending clinicians
my request to be present the autopsy; however, performance of the post-mortem examination will
not be delayed to accommodate such a request.
Brain cutting is scheduled as needed. The Pathology Department has a consultant
Neuropathologist, Dr. Solitaire, who provides comprehensive neuropathology services.
Stillbirth/Fetal Death:
If a fetus is born at a period of gestation of twenty weeks or greater and weighs 350gms or
greater, and there is no attempt at respiration, no action of the heart and no movement of
voluntary muscle, then such instances are recorded by the state as a fetal death, and a fetal death
certificate must be filed.
Any live birth (i.e., spontaneous respiration) will be treated as an adult death regardless of
gestational age, and a death certificate must be completed.
If a postmortem examination is requested for these cases, an autopsy permit must be completed
by the attending physician and the laboratory notified. The fetus is taken to the morgue and
security is also notified of a pending autopsy. If examination of the placenta is desired, the
placenta should be sent to the laboratory as a Routine Specimen and accompanied by a completed
Request for Placental Examination form.
For a stillbirth prior to twenty weeks of gestation or less than 350 gms., a fetal death certificate is
not filed. If a postmortem examination is desired, the fetus is sent to Histology for examination as
a Routine Specimen. It is not necessary to obtain an autopsy permit. If an autopsy permit has been
completed for a, then the submitting physician will be notified that the fetus will be examined in
Surgical Pathology and an autopsy permit is not necessary.
BLOOD BANK
The Blood Bank is located on 3.3 at extension 5110. It is under the direction of Nicole
Muscato, M.D. and is managed by Elizabeth DeRosa, BS, MT (ASCP)SBB. This section
is accredited by the College of American Pathologists (CAP), and is registered with the
Food and Drug Administration (FDA) and the State Department of Health. Blood Bank.
Orders are entered directly into the Hospital Information system (HIS). In case of
computer down time, a Universal Requisition is required for all orders. The requisitions
are obtained from the stockroom and are to be filled out at the nurse's station with the
following information:
1. Patient's demographic data
2. Check off necessary tests and indicate number of units
3. Priority - routine, STAT, today, outpatient date of transfusion or Preop
Packed Cell units that are crossmatched are held a maximum of 4 days from the time the
type and screen specimen was obtained (the only exception is for PAT specimens, see
below).
Definite transfusion order must include a Doctors Oders: Adult Blood Transfusion.
Outpatient transfusions must also be scheduled through the Outpatient Infusion
department or the Cancer Center. A completed and signed transfusion order from is faxed
to 860-444-5163.
Pre-Admission Testing Requisitions are required for preop orders. If the patient has not
been pregnant or transfused in the last 3 months, then this specimen can be used for
compatibility testing up to 21 days.
The hospital obtains blood and blood components from the American Red Cross Blood
Services in Farmington, CT. All donations are from volunteer donors and are tested with
licensed screening tests for viral markers as required by the FDA.
Besides blood and blood components, the Blood Bank also dispenses plasma derivatives
such as Factor VIII, Factor IX, Novoseven and Rh Immune Globulin. Refer to the
package inserts of specific products for more information.
Therapeutic phlebotomy appointments are scheduled and drawn by personnel in the
Outpatient Clinic (x2184). Blood obtained from therapeutic phlebotomies are labeled
"Not For Transfusion" and are appropriately discarded in the Blood Bank.
ABO and Rh Typing
Synonyms: Group & Rh, Blood Typing, Blood Grouping. Type & Rh, ABO/Rh
General Use: Identify blood type
Test Mnemonic: ABRH (forward & reverse typing) ABRHF (forward typing
only for neonates <4 months of age)
Test Includes: ABO and Rh Blood Types
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Positive patient identification
Specimen Required: K2EDTA Blood
Container Required: 6 ml K2EDTA Plasma Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Improper sample, improper labeling, excessive hemolysis
Availability: 24 hours daily
Time Required Routinely for Processing: 4-8 hours, STAT -1/2 hour
ABS see Antibody Screen
Albumin
Contact the pharmacy
Ante Partum RhIG, see Rh(D) Immune Globulin Antepartum
Antibody Screen
Synonyms: Indirect Antiglobulin Test, IAT, Indirect Coombs, Indirect AHG,
Antibody Detection, Atypical Antibody Screening; ABS
General Use: Detects atypical antibody(ies) in patient's serum
Test Mnemonic: ABSR
Test Includes: Antibody screen for Atypical Antibodies.
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Positive patient identification
Specimen Required: K2EDTA Blood
Container Required: 6 ml K2EDTA Plasma Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Improper labeling, excessive hemolysis, insufficient
volume, specimen more than 24 hrs old.
Availability: 24 hours daily
Time Required Routinely for Processing: 4-8 hours; STAT -3/4 hour
Additional Information: If antibody screen is positive, antibody identification
work up is performed.
Reference Range: Negative
Antibody Titer see Titer
Autologous Donors
Autologous Donations are no longer collected at Lawrence + Memorial Hospital.
For information on donating Autologous blood, please contact the American Red
Cross, CT region at: 860-678-2805
Blood Typing see ABO and Rh Typing
Compatibility Testing see Red Blood Cells - Crossmatch
Cord Blood
General Use: To evaluate newborn for hemolytic disease of the newborn (HDN)
and to determine mother's candidacy for Rh Immune Globulin.
Test Mnemonic: CDEV
Test Includes: ABO and Rh Typing and DAT
Lab Performing Test: Blood Bank
Request Form: Cord Blood (ABO/RH & Direct Coombs) - Signed order by
physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood
Container Required: 10 ml red top tube, no separator
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml
Routinely: CDEV is done on cord samples from group O mothers, Rh Negative
mothers and mothers with clinically significant atypical antibodies. Otherwise the
cord sample is held in the blood bank for 14 days.
Causes for Rejection: Improper labeling - must have patient name, hospital
number, date, mother's name and mother's hospital number
Availability: 24 hours daily
Time Required Routinely for Processing: 8 hours if routine; STAT - 1 hr
Cryoprecipitate
Synonyms: Cryoprecipitated Antihemophilic Factor, Cryoprecipitated AHF,
CRYO
General Use: Treatment of fibrinogen or factor XIII deficiency, or
dysfibrinogenemia.
Test Mnemonic: TCRY
Testing required: ABO and Rh Type, if not known
Lab Performing Test: Blood Bank
Request Form: A transfusion order faxed to the blood bank is required (860-4445163). Signed order by physician/Health Professional Affiliate(HPA)
specify # of units.
Patient Preparation: Proper patient identification. Must sign Informed Consent
for Transfusion
Special Instructions: Can be used for only 6 hours (after thawing the pool of 5).
Specimen Required: 2 independently collected ABO typings on file at L+M, or 1
pink top tube.
Container Required: 6 ml K2EDTA Plasma Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 2 ml
Availability: 24 hours daily
Time Required Routinely for Processing: 1/2 hour
Additional Information: Cryoprecipitate is supplied as a pool of 5 products. If
10 cryo units are ordered, 2 pools will be issued. They bags of 5 will not be
pooled together. Product contains factor VIII, factor XIII, von Willebrand's factor
and fibrinogen. Each unit contains approximately 10 ml of plasma.
Reference Range: Usual dose is 10 units (2 pools of 5)
Cryoprecipitated Antihemophitic Factor, Cryoprecipitated AHF, CRYO see
Cryoprecipitate
Direct Antiglobulin Test
Synonyms: Direct Coombs, DAT, Direct AHG Test
General Use: Detect antibody bound to patient's red cells
Test Mnemonic: DAT
Test Includes: Direct anti-human globulin test with polyspeciflc anti-human
globulin serum. It may include use of monospecific reagents (anti-IgG, and anti C3d, C3b) when indicated.
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Proper patient identification
Specimen Required: Whole blood (EDTA). In case of a newborn a cord blood
is used (see Cord Blood)
Container Required: 6 ml pink top tube or cord blood.
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 6 ml
Causes for Rejection: Improper labeling, specimen greater than 24 hrs. old.
Availability: 24 hours daily
Time Required Routinely for Processing: 4-8 hours; STAT- 1/2 hr
Additional Information: If the test with anti-IgG is positive, an eluate will be
performed to identify the antibody coating the cells
Reference Range: Negative
DAT see Direct Antiglobulin Test
Deglyced Red Cells see Red Blood Cells Deglycerolized - Crossmatch
Direct Coombs, see Direct Antiglobulin Test
FFP see Fresh Frozen Plasma
Fresh Frozen Plasma
Synonyms: FFP, 24 hr plasma
General Use: Treatment of coagulation factor deficiencies. NOT indicated for
condition responsive to volume replacements or when specific corrective factors
(factor VIII and/or IX) are available
Test Mnemonic: TFFP
Test Includes: ABO and Rh Type, if not known. Thawing frozen plasma
Lab Performing Test: Blood Bank
Request Form: A transfusion order faxed to the blood bank is required (860-4445163). Signed order by physician/Health Professional Affiliate(HPA) specify # of
units.
Patient Preparation: Proper patient identification. Must sign Informed Consent
for Transfusion
Special Instructions: Must be used within 24 hours after thawing
Specimen Required: 2 independently collected ABO typings on file at L+M or 1
pink top tube.
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml pink top tube
Minimum Volume of Specimen: 2 ml
Availability: 24 hours daily
Time Required Routinely for Processing: 1/2 hour
Additional Information: FFP contains plasma proteins including all coagulation
factors.
Frozen Red Cells see Red Blood Cells Deglycerolized - Crossmatch
Group & Rh, see ABO and Rh Typing
HLA-ABC
Synonyms: ABC typing for HLA
General Use: For Bone Marrow, Stem Cell or Platelet matching
Test Mnemonic: HLAABC
Lab Performing Test: American Red Cross, Connecticut Region, HLA lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Lavender Top Tube (EDTA)
Volume of Specimen: 12ml - draw 3 EDTA tubes
Minimum Volume of Specimen: 10ml
Availability: Draw any day of week; Sent Mon-Thursday
Time Required Routinely for Processing: 1 week
HLA-DRDQ
Synonym: DR typing for HLA
General Use: For Bone Marrow, Stem Cell or Platelet matching
Test Mnemonic: HLADRQ
Lab Performing Test: American Red Cross, Connecticut Region, HLA lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Lavender Top Tube (EDTA)
Volume of Specimen: 12ml - draw 3 EDTA tubes
Indirect Antiglobulin Test, see Antibody Screen
Indirect Coombs see Antibody screen
Packed Red Cells, see Red Blood Cells – Crossmatch Panel, see Antibody
Identification
Pediatric Packed Cells
General Use: Packed cell aliquot infusions should not exceed 4 hours. Mother’s
K2EDTA specimen may be used for antibody screen and crossmatch if unable to
obtain a clotted specimen from infants under 1 week of age.
Test Mnemonic: XM
Test Includes: Type and screen, if not already determined on current admission
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
and a transfusion order form faxed to 860-444-5163.
Patient Preparation: Positive patient identification
Special Instructions: This is only for neonates <4 months of age. After 4 months
order PC (Packed Cells)
Specimen Required: K2EDTA Blood
Container Required: 3 ml K2EDTA lavender top
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Improper labeling, excessive hemolysis
Availability: 24 hours daily
Time Required Routinely for Processing: 1 hour
Additional Information: Unless there is an extreme emergency, pedi units are
CMV negative, irradiated and negative for HgBs and issued in a syringe.
Phlebotomy see Therapeutic Phlebotomy
Platelet Concentrate
Synonyms: Platelet Packs; Platelets; Random Donor Platelets; Platelet
Transfusions, Pooled Platelets.
See also – Pre-Pooled platelets
General Use: To treat bleeding due to thrombocytopenia or platelet function
abnormality
Test Mnemonic: TPPL or TPPH (pheresis)
Test Includes: ABO and Rh Type, if not known
Lab Performing Test: Blood Bank
Request Form: Request must be phoned to the Blood Bank when the order is
initiated. Transfusion order faxed to the blood bank is required (860- 444-5163).
Signed order by physician/Health Professional Affiliate(HPA) specify number of
units requested.
Patient Preparation: Proper patient identification. Must sign Informed Consent
for Transfusion
Special Instructions: Platelets are no longer supplied by individual platelet
concentrates. Platelets are supplied and issued in pools of 5. They have a 5 day
expiration period. One pool of 5 is considered one dose.
Specimen Required: 2 independently collected ABO typings on file at L+M, or 1
pink top tube.
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Normal platelet count improper labeling of specimen
Additional Information: One dose of pooled platelets is expected to increase the
platelet count of an adult with a blood volume of 5000 ml about 35,000 to
50,000/mm3.
Reference Range: Usual adult dose 5 units pooled.
Platelet Packs see Platelet Concentrate
Pooled Platelets, see PrePooled Platelets
Prenatal Rhogam see Rh (D) Immune Globulin Antepartum
PrePooled Platelets
Synonyms: Platelet Packs; Platelets; Random Donor Platelets; Platelet
Transfusions, Pooled Platelets.
General Use: To treat bleeding due to thrombocytopenia or platelet function
abnormality. PrePooled Platelets contain 5 random donor platelets pooled
together in a closed system that maintains the 5 day expiration. The platelets are
pooled and tested for bacterial contamination by the blood supplier.
Test Mnemonic: TPPL
Test Includes: ABO and Rh Type, if not known
Lab Performing Test: Blood Bank
Request Form: Request must be phoned to the Blood Bank when the order is
initiated. A transfusion order faxed to the blood bank is also required (860-4445163). Signed order by physician/Health Professional Affiliate(HPA), specify
number of units.
Patient Preparation: Proper patient identification. Must sign Informed Consent
for Transfusion
Special Instructions: PrePooled Platelets have a 5 day expiration period.
Specimen Required: 2 independently collected ABO typings on file at L+M, or 1
pink top tube.
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Normal platelet count, improper labeling of specimen
Additional Information: One dose of prepooled platelets is expected to increase
the platelet count of an adult with a blood volume of 5000 ml about 35,000 to
50,000/mm3.
Reference Range: One dose = 5 random platelets
Random Donor Platelets see Pre-pooled platelets
Red Blood Cells - Crossmatch
Synonyms: Packed Red Cells, Compatibility Testing, Type and Crossmatch.
General Use: Transfusion of patients with symptomatic anemia for restoration of
oxygen - carrying capacity. Infusion should not exceed 4 hours per unit
Test Mnemonic: XM
Test Includes: ABO and Rh Type, antibody screen and crossmatch
Lab Performing Test: Blood Bank
Request Form: A transfusion order faxed to the blood bank is required (860-4445163). Signed order by physician/Health Professional Affiliate(HPA), specify
number of units.
Patient Preparation: Positive patient identification. Must sign Informed Consent
for Transfusion
Special Instructions: Crossmatched blood held for a maximum of 3 days.
Specimen Required: K2EDTA Blood
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Improper labeling, excessive hemolysis, insufficient
volume, specimen more than 24 hrs. old.
Availability: 24 hours daily
Time Required Routinely for Processing: 10 minutes with completed Type and
Screen, otherwise 45 minutes
Additional Information: Blood available uncrossmatched in emergency per
physicians order. Release form for uncrossmatched blood is initiated by Blood
Bank and must be signed by the physician.
Red Blood Cells Deglycerolized - Crossmatch
Synonyms: Frozen Red Cells; Deglyced Red Cells; Washed Cells
General Use: Only used for patients with difficult compatibility problems or
severe reactions to plasma proteins
Test Mnemonic: XM
Test Includes: Group, Rh, antibody screen & compatibility testing
Lab Performing Test: Blood Bank and Connecticut Red Cross Blood Services
Lab Request Form: A transfusion order faxed to the blood bank is required
(860-444-5163). Signed order by physician/Health Professional Affiliate(HPA).
Patient Preparation: Proper patient identification. Must sign Informed Consent
for Transfusion
Specimen Required: K2EDTA Blood
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Improper labeling, excessive hemolysis, insufficient
volume, specimen more than 24 hrs old.
Availability: Not STAT, weekdays. Product must be obtained on special order
from American Red Cross Blood Services. Special transportation needed.
Time Required Routinely for Processing: 24 hours
Additional Information: Product when thawed has a 24-hour shelf life.
Red Blood Cells Leukocyte Reduced - Crossmatch
Synonyms: Buffy Coat Poor Blood, White Cell Poor Blood
General Use: To prevent recurrent febrile, non-hemolytic transfusion reactions or
HLA alloimmunization.
Test Mnemonic: XM
Test Includes: ABO and Rh Type, Antibody screen and crossmatch
Lab Performing Test: Blood Bank
Request Form: A transfusion order faxed to the blood bank is required (860-4445163). Signed order by physician/Health Professional Affiliate(HPA).
Patient Preparation: Proper patient identification
Special Instructions: Must sign Informed Consent for Transfusion
Specimen Required: K2EDTA Blood
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: improper labeling, excessive hemolysis, insufficient
volume, specimen more than 24 hrs old.
Availability: 24 hours daily
Time Required Routinely for Processing: 10 minutes with completed TYSC;
otherwise 45 minutes
Additional Information: Red Cells are filtered pre-storage at the American Red
Cross through blood filters that remove >99% of the White Cells.
Rh Titer, Antibody Titer, Antibody Quantitation, Atypical Antibody Titer
see Titers
Rh(D) Immune Globulin
Synonyms: RhoGam; RhIG
General Use: Given to D-negative women to prevent development of Anti-D and
hemolytic disease of newborn in subsequent pregnancies
Test Mnemonic: TRHG
RHEV (full work up, including antibody screen)
Test Includes: Fetal screen to detect fetal-maternal hemorrhage greater than 30
ml
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Proper patient identification
Special Instructions: Blood required from both mother and baby
Specimen Required: Clotted blood from infant; EDTA tube from mother
Container Required: 10 ml red top tube (no separator) from the cord, and a 6 ml
Pink Top for mother or capillary tube for newborn
Volume of Specimen: 10 ml red top tube, cord sample(no separator) and a 6 ml
Pink Top from mother. If cord is unavailable, capillary tubes from heel stick
Minimum Volume of Specimen: 3 ml for mother, 1 ml for newborn
Causes for Rejection: Baby Rh-negative, mother D-positive, or active anti-D
present in maternal serum
Availability: Routine days
Time Required Routinely for Processing: Up to 4 hours
Additional Information: Dosage of Rh(D) immune globulin is determined by a
fetal screen done by Blood Bank on postpartum maternal blood. If fetal screen is
positive, a quantative test is done by the Hematology.
Rh(D) Immune Globulin Antepartum
Synonyms: Antepartum RhIG, Prenatal Rhogam
General Use: Given to Rh-negative women to prevent the development of anti-D
and hemolytic disease of newborn in subsequent pregnancies Test Mnemonic:
TRHG
Test Includes: Antibody Screen as ordered
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Proper patient identification
Special instructions: Given to D-negative mothers during the 26th - 28th week of
pregnancy, who have not previously produced anti-D
Specimen Required: K2EDTA Blood
Container Required: 6 ml Pink Top
Volume of Specimen: 6 ml from patient
Minimum Volume of Specimen: 5 ml
Collection: Collect specimen before dose of RH(D) Immune Globulin is given
Causes for Rejection: If patient is D-positive, or an active, immune anti D is
detected in the mother’s plasma.
Availability: Routine
Contraindications: If mother known D-Positive, or has produced Anti-D
RhIG see Rh(D) Immune Globulin
RhoGam see Rh(D) Immune Globulin
Salt Poor Albumin, Normal Serum Albumin, 25% Serum Albumin, 5% Albumin
see Albumin Product
Contact the pharmacy
Therapeutic Bleeding see Therapeutic Phlebotomy
Therapeutic Phlebotomy
Synonyms: Therapeutic Bleeding; Phlebotomy
Test Mnemonic: PHLEB
Test Includes: Drawing up to 450 ml of blood from patient
Lab Performing Test: Blood Bank or Outpatient Clinic (by appointment) phone
# Ext 2184
Request Form: Patient must have a physician order. New standing orders are
required every 12 months. Signed order by physician/Health Professional
Affiliate(HPA).
Patient Preparation: Hemoglobin or hematocrit determination on chart less than
48 hours old. Must sign consent for the procedure. Vital signs must be
documented immediately prior.
Special Instructions: Outpatient appointments made with Outpatient Clinic 4420711 Ext #2184.
Container Required: Blood donor bag
Volume of Specimen: Up to 500 mL
Causes for Rejection: Discretion of pathologist
Availability: Weekdays only 8:00AM -4:30PM; emergency therapeutic
Phlebotomies cleared through a pathologist
Time Required Routinely for Processing: 1 hour
Titer
Synonyms: Rh Titer, Antibody titer, Antibody Quantitation, Atypical Antibody
Titer
General Use: To quantitate antibody
Test Mnemonic: ATIT (only orderable by blood bank staff.)
Order ABSR or PREN1
Test Includes: Antibody screen (detection), identification and antibody titer
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Positive patient identification
Specimen Required: K2EDTA Blood
Container Required: 6 ml Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 4 ml
Causes for Rejection: Improper labeling, excessive hemolysis, insufficient
volume, specimen more than 24 hrs old.
Availability: Routine weekdays
Time Required Routinely for Processing: Up to 8 hours
Additional Information: Titers are only be done if antibody is present in
patient's plasma and is considered clinically significant and the patient is
pregnant.
Transfusion Reaction Review
General Use: Investigate transfusion problems
Test Mnemonic: TRXRX
Test Includes: DAT, Compatibility Studies, Urine for Blood and Chemistry
Studies as necessary. Workup may be modified by Blood Bank in accordance
with AABB/FDA/CAP guidelines.
Lab Performing Test: Blood Bank
Request Form: Lawrence + Memorial Hospital Suspected Transfusion Reaction
Report (L+M- 278 Lab)
Patient Preparation: Positive patient identification
Special Instructions: Follow instructions on back of the product. Attending
physician must be notified prior to calling Blood Bank
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml K2EDTA
Minimum Volume of Specimen: 6 ml pink top
Availability: STAT, 24 hours daily
Time Required Routinely for Processing: Varies with type of reaction reported
Specimen Required: Post transfusion pink top tube.
Additional Information: Blood container must be sent to Blood Bank with the
Transfusion Record, include tubing and saline bag.
Reference Range: N/A
Type & Screen
Synonyms: Cells and Serum, Gr, Rh & Scr
General Use: Required for Pre-transfusion testing. Determination of ABO/Rh
and possible presence of an atypical antibody.
Test Mnemonic: TYSC
Test Includes: ABO and Rh Type and antibody screen
Lab Performing Test: Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Positive patient identification
Specimen Required: K2EDTA specimen
Container Required: 6 ml K2EDTA Pink Top
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Improper labeling, excessive hemolysis, insufficient
volume, specimen more than 24 hrs old.
Availability: 24 hours daily
Time Required Routinely for Processing: 4-8 hours; STAT - hour
Additional Information: Decreases crossmatch time should transfusion become
necessary. If antibody screen is positive, antibody identification will be done. For
npatients with antibodies, 2 units will be made available until the specimen
expires.
Washed Cells see Red Blood Cells Deglycerolized - Crossmatch
Reference Lab
The Reference Lab section is directed by Victoria G. Reyes-D'Arcy, M.D. and supervised
by Barbara Naillis. The Section is staffed Monday through Friday from 6:30 am to 11
pm. Limited services are available on weekends and holidays from 7 am to 3 pm.
For Reference Lab services call ext 4153. The Supervisor can be reached at ext 3780.
1st Trimester Risk Screen
General Use: Screening for fetal chromosome abnormalities.
Test Mnemonic: FRSTTM
Test Includes: PAPP-A, AFP, HcG, Inhibin, Estriol
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient is drawn in first trimester of pregnancy
Special Instructions: Yale “Risk History” form needed at time of specimen
collection.
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: No patient History from
Availability: Can be drawn Mon-Sunday. Only sent to Yale Mon-Friday (no
holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
5-Hydroxyindolacetic Acid, Quantitative, 24 hour Urine
Synonyms: 5HIAA-Quantitative
General Use: Evaluate serotonin production as with carcinoid tumors
Test Mnemonic: U5HI24
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should avoid food high in indoles: avocados,
banana, tomato, plum, walnut, pineapple, and eggplant. Patient should also avoid
tobacco, tea and coffee three days prior to specimen collection.
Specimen Required: 24 hour urine
Special Instructions: Keep refrigerated during collection
Container Required: 24 hour urine container with preservative obtained from
Specimen Processing Laboratory
Volume of Specimen: Entire 24 hour collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
5' Nucleotidase, Blood
General Use: Parallels the activity of alkaline phosphatase in liver disease but is
not elevated in Rickets or Paget's disease.
Test Mnemonic: 5NUC
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube, No gel barrier tubes!, Deliver immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Improper tube type, delay in transport to Lab
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-4 days
Reference Range: See report
17-Hydroxyprogesterone
General Use: 17-Hydroxyprogesterone is useful in detecting patients with
congenital adrenal hyperplasia (CAH), monitoring cortisol replacement therapy,
evaluating infertility and adrenal and overarian neoplasms.
Test Mnemonic: 17HYDR
Test Includes:
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation:
Special Instructions:
Specimen Required: Clotted blood (serum)
Container Required: Red top tube, No gel
Volume of Specimen: 1.0 mL serum
Minimum Volume of Specimen: 1.0 mL serum
Causes for Rejection: SST Tube
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
17-Ketosteroids, Total, 24 hour Urine
Synonyms: 17-KS
General Use: Evaluate Androgenic function
Test Mnemonic: U17K24
Lab Performing Test: Specimen Processing; sent Reference Lab
Request Form: Universal requisition; use as back up to Meditech
Specimen Required: 24 hour urine
Special Instructions: Keep refrigerated during collection
Container Required: 24 hour urine container with preservative supplied by
Specimen Processing Laboratory.
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire 24 hr collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-7 days
Reference Range: See report
Acetylcholine Receptor Antibody
Synonyms: ARA
General Use: Useful in the diagnosis of Myasthenia gravis
Test Mnemonic: ACTYAB
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 5.0 mL
Minimum Volume of Specimen: 2 mL
Causes for Rejection: Excessive hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Additional Information: May also be ordered as part of a Myasthenia gravis
Panel
Reference Range: See report
Adenovirus Titer
General Use: Used to support a diagnosis of viral infection due to Adenovirus
Test Mnemonic: ADEN
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 2 ml. serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Sent out Mon-Sat. (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See printed report
Adrenocorticotrophic Hormone
Synonyms: ACTH; Corticotrophin
General Use: Pituitary function test useful in the differential diagnosis of
Cushing's syndrome, Addison's disease and also in ectopic ACTH syndrome
Test Mnemonic: ACTH
Lab Performing Test: Specimen Processing - sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting recommended
Special Instructions: Sample should be drawn in am (7-10AM) - Deliver to lab
immediately on ice. Commonly collected simultaneously with cortisol level.
Specimen Required: Plasma (EDTA)
Container Required: Lavender (EDTA) tube. Draw in pre-chilled tube, place on
ice, and deliver ASAP!
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 1 ml plasma
Causes for Rejection: Gross hemolysis
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 4 days
Additional Information: The ACTH level is affected by stress, which may
obscure the normal diurnal change. ACTH level should be correlated with cortisol
levels.
Reference Range: See report
AFFIRM VP DNA Probe Panel
Synonym: Bacterial Vaginosis/Vaginitis Panel
General Use: Testing for organisms associated with bacterial vaginosis
Test Mnemonic: AFFIRM
Test Includes: Candida Species, Trichomonas vaginalis, Gardnerella
vaginalis
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form:
Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Vaginal swab
Container Required: AFFIRM VPIII Ambient Temp Transport System
(ATTS)
Volume of Specimen: One swab
Minimum volume: One swab
Causes for Rejection: Specimens > 72 hrs old, frozen specimens or
swabs received in transport systems other than AFFIRM
Availability: Sent out Monday-Saturday (not holidays)
Time Required Routinely for Processing: One week
Additional Information: Cervical specimens should be avoided,
specimens should not be collected if patient has used vaginal products
in the previous 72 hours
Reference Range: Not detected
Aldolase, Blood
Synonym: Fructose-1,6 bisphosphate
General Use: Differential diagnosis of cellular necrosis, cartilage disorder, acute
hepatitis, progressive muscular dystrophy, myocardial infarction, pancreatitis
Test Mnemonic: ALDO
Lab Performing Test: Specimen Processing - sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Gross hemolysis, icterus, lipemia
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 2 days
Reference Range: See report
Aldosterone, 24 hour Urine
General Use: Determining adrenal cortical function and renal hypertension
Test Mnemonic: UALD24
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep refrigerated during and after collection
Specimen Required: 24 hour urine collection
Container Required: 24 hour urine container with 10gms boric acid, supplied by
Specimen Processing lab
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See report
Aldosterone, Serum
General Use: Evaluation of adrenal function
Test Mnemonic: ALDS
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Draw “upright” samples at least ½ hour after patient sits up
Specimen Required: Clotted blood (serum)
Container Required: Plain Red top – no gel barrier!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: SST tube
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See report
Alkaline Phosphatase Isoenzymes, Blood
Synonyms: Alkaline Phosphatase Fractionation
General Use: Differentiate liver disease and bone disease
Test Mnemonic: ALKISO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Patient Preparation: Overnight fasting preferred
Special Instructions: Age and sex of patient are necessary for interpretation of
results
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum). Spin within 30 min
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 3 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 2-5 days
Reference Range: See report
Alkaline Phosphatase, Bone Specific
General Use: Provides a general index of bone turnover, formation and a specific
index of total osteoblast activity.
Test Mnemonic: ALKIBS
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, icterus, lipemia and non-serum
specimens
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Alpha - 1 - Antitrypsin, Blood
General Use: Diagnosis of alpha- antitrypsin deficiency
Test Mnemonic: A1AT
Lab Performing Test: Specimen Processing - sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting preferred
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Lipemic samples not accepted
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See report
Alpha feto protein, maternal
Synonym: Quad screen, AFP, AFETO
General Use: Screening for Down’s Syndrome and Neural tube defects
Test Mnemonic: AFETO
Test Includes: AFP, HcG, Inhibin and Estriol
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Sample must be drawn between 15 weeks 0 days and 22
weeks 6 days.
Special Instructions: Prenatal Assay form (History form) must accompany the
specimen.
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: No patient history form
Availability: Specimen may be drawn Mon-Sunday. Only sent to Yale MonFriday (no holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Alprazolam
Synonym: Xanax
General Use: Alprazolam is an anti-anxiety agent of the benzodiazepine class. It
is indicated for the management of anxiety disorders and for anxiety associated
with depression.
Test Mnemonic: ALP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Protect from light, Deliver ASAP, Keep at Room
temperature!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Drawn in tube with gel barrier (SST) or any serum
separator (SST). Received not protected from light.
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Aluminum, Blood
General Use: Determining aluminum toxicity
Test Mnemonic: ALUM
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should refrain from taking antacids containing
aluminum compounds at least three days prior to sample collection.
Specimen Required: Plasma
Container Required: Navy top tube (EDTA)
Volume of Specimen: 1 Navy (EDTA) tube
Minimum Volume of Specimen: 2 ml plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Amino Acids - Quantitative Plasma
General Use: Evaluation of metabolic disorders
Test Mnemonic: AAS
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: plasma
Container Required: green top tube (Li or Na heparin)
Volume of Specimen: 6 or 10 ml of whole blood
Minimum Volume of Specimen: 1.0 ml plasma
Availability: Sent Monday-Friday (no weekends or holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Amino Acids - Quantitative Urine
Synonyms: Amino Acid Screen
General Use: Evaluation of metabolic disorders
Test Mnemonic: UAA
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Random urine
Container Required: Standard urine collection container (urine)
Volume of Specimen: Entire collection
Minimum Volume: 5 ml random urine
Availability: Sent Monday-Friday (no weekends or holidays)
Time Required Routinely for Processing: 2 Days
Reference Range: See report
Amino Levulinic Acid, 24 hour Urine
Synonym: ALA
General Use: Evaluate porphyrias and lead poisoning
Test Mnemonic: UALA24
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Avoid exposure of specimen to light during & after
collection. Refrigerate during and after collection.
Specimen Required: 24 hr collection
Container Required: Brown urine specimen container from lab (no preservative)
Volume of Specimen: Entire specimen collection
Minimum Volume of Specimen: 24 hr. entire specimen
Availability: Sent Monday- Saturday (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See report
Amiodarone
General Use: Therapeutic drug monitoring
Test Mnemonic: AMIO
Test Includes: Amiodarone and Desethylamiodarone (metabolite)
Lab Performing Test: Specimen Processing, sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliverer to Lab immediately!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Cause for Rejection: Improper tube, gross hemolysis
Availability: Sent Mon. - Saturday (no holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Amitriptyline
Synonyms: Elavil, Lambitrol
General Use: Monitor therapeutic drug level
Test Mnemonic: AMI
Test Includes: Amitriptyline, Nortriptyline, combined total (calculated)
Lab Performing Test: Specimen Processing - sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Collect as trough or at least 12 hours after last dose
Specimen Required: Clotted blood (Serum)
Container Required: Red top with no gel barrier!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Drawn in tube with gel barrier or any serum separator
(SST), Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See report
Amniotic Fluid L/S Ratio
Test Mnemonic: LS
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Amniotic fluid
Minimum Volume of Specimen: 3 ml of fluid
Time Required Routinely for Processing: Results ready same day or the
following day, depending on time specimen is received in lab.
Reference Range: See Report
Amphetamines, Urine
Test Mnemonic: UAMPH
Test Includes: Amphetamine, Methamphetamine
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Random urine
Container Required: Urine Cup
Volume of Specimen: 20 mL urine
Minimum Volume of Specimen: 20 mL urine
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Amylase Isoenzymes
Synonym: Salivary Amylase
Test Mnemonic: AMYISO
Test Includes: Total Amylase, Pancreatic Isoenzyme, and Salivary Isoenzymes
(calculated)
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Anaplasma phagocytophilum Antibody
Synonym: Ehrlichia, HGE
General Use: Used to support a diagnosis of Ehrlichiosis
Test Mnemonic: ANAPLA
Test Includes: Testing for E. chaffeensis and E. equi
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Time Required Routinely for Processing: 5 days
Reference Range: Interpretation provided with test results
Androstenedione
General Use: Androstenedione may be useful in evaluating patients with
androgen excess and managing patients with congenital adrenal hyperplasia
(CAH).
Test Mnemonic: ANDR
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Early morning specimen preferred
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Drawn in tube with gel barrier or any serum separator
(SST)
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Angioedema Panel, Acquired
Test Mnemonic: ANGIOP
Test Includes: C1 Inhibitor, Functional; C1 Inhibitor, Protein; C1q Complement
Component
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferrd
Special Instructions: Early morning specimen preferred
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Drawn in tube with gel barrier or any serum separator
(SST)
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Angiotensin Converting Enzyme
General Use: Useful in diagnosis of sarcoidosis
Test Mnemonic: ACE
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 2.0 ml
Minimum Volume of Specimen: 1.0 ml serum
Causes for Rejection: Gross Hemolysis and EDTA plasma
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 2 days
Reference Range: See report
Anti-Cardiolipin Antibody
Synonyms: Cardiolipin Antibodies
General Use: Elevations may be predictive of risk of thrombosis or recurrent
spontaneous abortions of early pregnancy. Also present in SLE patients with
arterial and venous thrombosis
Test Mnemonic: ACARD
Test Includes: Anti-cardiolipin IgG, IgM and IgA
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Citrated Plasma
Container Required: Light blue top
Volume of Specimen: 1.0 ml
Minimum Volume of Specimen: 1.0 ml
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See report
Antidiuretic Hormone
Synonym: Arginine Vasopressin (AVP)
General Use: Diagnosis of electrolyte imbalances; screening for fluid regulation
and renal function
Test Mnemonic: ADH
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver immediately to Specimen Processing lab
Specimen Required: Whole blood (EDTA)
Container Required: Lavender top tube (2) (EDTA)
Volume of Specimen: 4 mL EDTA plasma
Minimum Volume of Specimen: 2 mL EDTA plasma
Causes for Rejection: Delay in receipt of sample
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 9 days
Reference Range: See report
Anti-DNA, Single Stranded
Synonyms: ssDNA
General Use: Abs to single stranded DNA may be present in patients with SLE
and other autoimmune diseases
Test Mnemonic: SSDNA
Test Includes: ssDNA IgG
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST), deliver to Lab immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Delay in receipt of sample
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Anti Strep DNase-B
Synonyms: Anti-deoxyribonuclease B
General Use: Diagnostic of recent or previous infection with Beta hemolytic
streptococci
Test Mnemonic: ADNB
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4-7 days
Reference Range: Varies according to age of patient reference ranges provided
with results
Anti-Epidermal Antibody
Synonyms: Anti-skin (dermal-epidermal) Antibody
General Use: Present in 80% of patients with bullous pemphigoid.
Test Mnemonic: AEAB
Test Includes: Differentiation of Abs to intercellular substance and basement
membrane zone
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, icterus, or lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Anti-Glomerular Basement Membrane
Synonyms: Anti-GBM
General Use: High titers suggestive of Goodpasture's disease or Anti-GBM
nephritis
Test Mnemonic: AGBM
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: Negative
Anti-Mitochondrial Antibody
General Use: Diagnostic for primary biliary cirrhosis
Test Mnemonic: AMA
Lab Performing Test: Specimen Processing; Sent to Reference Laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See report
Anti-Neutrophil Cytoplasmic Antibody
Synonyms: ANCA
General Use: To support the diagnosis of Wegener's granulomatosis, Good
Pasture's disease
Test Mnemonic: ANCA
Lab Performing Test: Specimen Processing;Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Antinuclear Antibody
General Use: Screening test for Autoimmune diseases, connective tissue diseases
Test Mnemonic: ANASR
Lab Performing Test Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml. serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon. - Fri, 7:00 AM-3:00PM ; Not Stat
Time Required Routinely for Processing: 2-3 days
Reference Range: < 1:40. ANA pattern reported with positive results
Antiplatelet Antibody
General Use: Determine presence of antibody to platelets
Test Mnemonic: ANTPLT
Test Includes: IgG Indirect
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Wrong collection tube, mislabeled or unlabeled specimen.
Availability: Sent out Mon – Sat (not holidays)
Reference Range: See printed report
Anti-Streptolysin O Titer
Synonyms: Streptolysin-O Antibody, ASO, Strep A, Streptococcus, Group A,
Strep
General Use: To detect presence of antibodies to Streptolysin-O
Test Mnemonic: ASOAB
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemic
Availability: Sent out Mon - Sat (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See report
Apolipoprotein A1
General Use: Apolipoprotein A1, has been reported to be a better predictor of
CAD than HDL and Triglycerides
Test Mnemonic: APOA1
Lab Performing Test: Specimen Processing – Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting for at least 12 hours is required
Specimen Required: Clotted blood (serum)
Container Required: Red top (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross Lipemia, Plasma
Availability: Sent Monday – Saturday (no holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Apolipoprotein B
General Use: Indication of CAD. In some patients APOB is elevated even in the
presence of normal LDL
Test Mnemonic: APOB
Lab Performing Test: Specimen Processing- Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must be fasting
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (No gel)
Volume of Specimen: 10ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis or lipemia, Plasma
Availability: Sent Monday-Saturday (no holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Apolipoproteins A1&B
General Use: Apolipoprotein A1 is the primary protein associated with HDL
cholesterol. Like HDL cholesterol, increased concentrations are associated with
reduced risk of cardiovascular disease. Apolipoprotein B-100 is the primary
protein associated with LDL cholesterol and other lipid particles. Like LDL
cholesterol, increased concentrations are associated with increased risk
of cardiovascular disease. The ratio of these two apolipoproteins correlates with
risk of cardiovascular disease.
Test Mnemonic: APO
Test Includes: Apolipoprotein A1, Apolipoprotein B, Apolipoprotein B/A1 ratio
(Calculated)
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting required – 12-16 hours
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis and lipemia, Plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3 days
Additional Information: Apolipoprotein A1 & Apolipoprotein B can each be
ordered separately (Mnemonics: APOA1 & APOB)
Reference Range: See Report
Acetylcholine Blocking Antibody
General Use: Useful in the diagnosis and management of Myastenia Gravis
Test Mnemonic: ACYBAB
Lab Performing Test: Specimen Processing; Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (No gel)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: Reference range and interpretation is provided with test results
Arsenic, 24 hour urine
Synonyms: As
General Use: Arsenic poisoning, arsenic toxicity
Test Mnemonic: UARS24
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Specimen must not be voided into a metal container!
Patient should refrain from eating shellfish, shrimp, crab, lobster and bottomfeeders such as flounder at least three days prior to specimen collection.
Special Instructions: Keep refrigerated during collection
Specimen Required: Entire 24 hour urine collection
Container Required: 24 hr acid washed container obtained from Specimen
Processing Lab
Volume of Specimen: Entire 24 hour collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Arsenic, Blood
General Use: Arsenic toxicity
Test Mnemonic: ARS
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Pt should refrain from seafood and herbal supplements for
three days prior to blood collection.
Special Instructions: Do not spin tube!!!
Specimen Required: Whole blood
Container Required: Navy top (EDTA)
Volume of Specimen: 7 ml
Minimum Volume of Specimen: entire collection
Causes for Rejection: Improper tube type, centrifuged tube, gross hemolysis
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 3 days
Additional Information: Arsenic may only be present in blood for 4-6 hours.
This test is most accurate in acute poisoning.
Reference Range: See Report
ASCA (Saccharomyces Cerevisiae) IgA
Synonym: anti-Saccharomyces cerevisiae antibodies (ASCA) of the IgA class
General Use: The presence of ASCA (S. cerevisiae) IgA antibodies, used in
conjunction with clinical findings and other laboratory tests, may aid in
the diagnosis of patients with Crohn's disease. ASCA (S. cerevisiae) IgA
ELISA should be used to complement, but not to replace or to substitute for
ASCA IgG antibody testing.
Test Mnemonic: ASCAIA
Test Includes: Semi-quantitative detection of anti-Saccharomyces
cerevisiae antibodies (ASCA) of the IgA class
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
ASCA (Saccjaromyces Cerevisiae) IgG
Synonym: anti-Saccharomyces cerevisiae antibodies (ASCA) of the IgG class
General Use: This test is intended to aid in the diagnosis of patients with Crohn's
disease.
Test Mnemonic: ASCAIG
Test Includes: enzyme-linked immunosorbent assay (ELISA) for the semiquantitative detection of S. Cerevisiae (ASCA) IgG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Aspergillus fumigatus IgG
General Use: Useful to confirm exposure to or infection by Aspergillus
fumigatus
Test Mnemonic: ASPIGG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10ml
Minimum Volume of Specimen: 0.5 ml serum
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 4-5 days
Reference Range: See Report
Aspergillus, Serology
General Use: Aid in the diagnosis and prognosis of infections due to Aspergillus
sp. and in the planning of a fungus culture schedule
Test Mnemonic: ASPERP
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Additional Information: This test is also available in the Fungal Serology Panel
Reference Range: Negative
Babesia Serology
Synonym: Babesia microti
General Use: To aid in the diagnosis of infection with Babesia Microti
Test Mnemonic: BABS
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Barbiturates, Total, Blood
General Use: Barbiturate toxicity
Test Mnemonic: BARB
Test Includes: Amobarbital, Butabarbital, Butalbital, Pentobarbital,
Phenobarbital, Secobarbital
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: 2 Red top tubes; No gel barrier tube!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Improper tube type
Availability: Monday-Saturday (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See report
Beta-2-Glycoprotein (IgG, IgA, IgM)
General Use: Beta-2-Glycoprotein 1, apolipoprotein H, is a cofactor in
antiphospholipid antibody binding and is the critical antigen in the
antiphospholipid antibody syndrome. Beta-2-Glycoprotein 1 Antibody is more
specific than Cardiolipin Antibody that may express reactivity in patients with
syphilis and other infectious diseases.
Test Mnemonic: B2GLY
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Plasma
Container Required: 2 blue top (Sodium Citrate) tubes
Volume of Specimen: 2 blue top (Sodium Citrate) tubes
Minimum Volume of Specimen: 3 ml plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Beta-2-Microglobulin
General Use: Used as an indicator of glomerular function Elevated levels also
noted in neoplasms.
Test Mnemonic: B2M
Lab Performing Test: Specimen Processing- Sent to referring lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Causes for Rejection: Improper tube, gross hemolysis, lipemia
Availability: Sent Mon-Saturday (no holidays)
Reference Range: See report
Blastomyces, Serology
General Use: Aid in the diagnosis and prognosis of infections due to
Blastomyces Dermatitidis and in the planning of a fungus culture schedule
Test Mnemonic: BLAS
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: This test is also available in the Fungal Serology Panel.
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Blood Chromosome Analysis
Synonym: Karyotype
General Use: Physician interpretation
Test Mnemonic: BLCR
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Diagnosis required – ICD.9
Specimen Required: Whole blood
Container Required: Green top tube (sodium heparin)
Volume of Specimen: 10.0 ml of blood (green top tube)
Minimum Volume of Specimen: 3.0 ml
Causes for Rejection: Clotted specimen, incorrect specimen volume, mislabeled
or unlabeled specimen.
Availability: Daily Mon-Fri: not STAT, not available on weekends
Time Required Routinely for Processing: 2-3 weeks
Reference Range: Interpreted by physician
Chromosomes, Bone Marrow or Peripheral Blood
Synonyms: Bone Marrow Karyotype
General Use: Interpreted by physician
Test Mnemonic: KARYO
Lab Performing Test: Specimen Processing, sent to Reference lab
Request Form: Bone Marrow Request form (filled out in entirely by physician)
Patient Preparation: Performed by physician
Special Instructions: Call Hematology for bone marrow tray
Specimen Required: Bone marrow aspirate or peripheral blood
Container Required: Green top tube (sodium heparin) or EDTA Lavender top
Volume of Specimen: 3.0 ml
Minimum Volume of Specimen: 2.0 ml
Causes for Rejection: Clotted specimen, incorrect specimen volume, mislabeled
or unlabeled specimen.
Availability: Daily Mon-Fri.
Time Required for Processing: 21 days
Reference Range: See charted report
Bordetella Pertussis Antibodies
Synonym: Bordetella Antibodies
General Use: Used to establish evidence of infection/exposure to Bordetella
pertussis, the causative agent of whooping cough.
Test Mnemonic: BORDPAB
Test Includes: Pertussis toxin IgG, Pertussis toxin IgA, Filamentous
Hemagglutinin Antigen IgG, Filamentous Hemagglutinin Antigen IgA
Lab Performing Test: Specimen Processing, sent to Reference lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Special Instructions: none
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Excessive hemolysis
Availability: Sent out Monday thru Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
C1 Esterase, Functional
General Use: Functional C1-Inhibitor, C1 Esterase
Test Mnemonic: C1FUNC
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver to lab immediately!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube- No gel tube!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Improper tube type, gross hemolysis and lipemia
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
C1 Esterase Inhibitor
General Use: A deficiency of this protein is characteristic of hereditary
angioedema
Test Mnemonic: C1EST
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred
Special Instructions: Collect on ice. Deliver immediately to Specimen
Processing
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, delay in delivery to Lab
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See report
C3d Circulating Immune Complexes
General Use: Circulating immune complexes (CIC) are present in many
individuals with systemic lupus erythematosus (SLE) and rheumatoid arthritis
(RA), especially with any of the vasculitides complications. Levels of CICs have
been reported to show correlation with disease activity in that higher levels are
reported during active phases of the disease.
Test Mnemonic: C3DIGG
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (No Gel)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: SST tube, delay in delivery to Lab
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See report
CA15-3
General Use: Useful for monitoring therapy and confirming biopsy of certain
carcinomas
Test Mnemonic: CA153
Lab Performing Test: Specimen Processing, sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume Required: 2 ml
Causes For Rejection: Gross hemolysis or lipemia
Availability: Sent out Monday through Saturday (not holidays)
Reference Range: See patient report
CA19-9
Synonym: Carbohydrate Antigen 19-9
General Use: Useful for monitoring therapy and confirming biopsy of certain
carcinomas
Test Mnemonic: CA19
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
CA27.29
General Use: Useful in monitoring therapy and confirming biopsy of certain
carcinomas
Test Mnemonic: CA27
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Improper tube type, Gross hemolysis
Availability: Sent out Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Caffeine
General Use: Therapeutic drug monitoring
Test Mnemonic: CAF
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube- SST
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 0.5 ml serum
Availability: Sent Mon-Saturday * Stats only on Sat.
Time Required Routinely for Processing: 2-3 days for routine
Reference Range: See Report
Calcitonin
Synonyms: Thyrocalcitonin
General Use: Early detection of medullary carcinoma of the thyroid gland;
evaluation of calcium metabolism
Test Mnemonic: CALC
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting preferred
Special Instructions: Deliver immediately to Laboratory!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Delay in receipt of specimen, Gross hemolysis
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See report
Candida albicans Antibodies
General Use: Aid in the diagnosis and prognosis of Candida fungal infections
and in the planning of a fungus culture schedule
Test Mnemonic: CANALB
Test Includes: IgG, IgM, IgA
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See Report
Carbamazepine 10, 11 Epoxide
General Use: Carbamazepine and its metabolite (10, 11-Carbamazepine epoxide)
are widely used for control of generalized tonic-clonic, partial-onset, complex and
mixed seizure disorders.
Test Mnemonic: CARBEP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: SST tube
Time Required Routinely for Processing: 5 days
Reference Range: See Report
Carotene
General Use: Diagnosis of malabsorption syndromes
Test Mnemonic: CARO
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Protect specimen from light by wrapping in aluminum foil
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Refrigerated specimens, exposure of specimen to light
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 Days
Reference Range: See report
Cat Scratch Fever Serology
Synonyms: Bartonella Abs
General Use: To aid in the diagnosis of Cat Scratch disease
Test Mnemonic: BARTAB
Test Includes: Bartonella henselae IgG, IgM; Bartonella quintana IgG, IgM.
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Catecholamines, 24 hour Urine
General Use: Evaluation of catecholamine production
Test Mnemonic: UCAT24
Test Includes: Urine Dopamine, Epinephrine, Norepinephrine, and total
catecholamine (calculated)
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: It is preferable for the patient to be off medications for
three days prior to collection. However, common antihypertensives (diuretics,
ACE inhibitors, calcium channel blockers, alpha and beta blockers) cause
minimal or no interference. Patient should avoid alcohol, coffee, tea, tobacco
(including use of nicotine patch), bananas, citrus fruits and strenuous exercise
prior to collection.
Special Instructions: Refrigerate during collection
Specimen Required: 24 hr urine
Container Required: 24 hour urine container with 25ml 6N HCL obtained from
Specimen Processing laboratory.
Volume of Specimen: Entire 24 hr collection
Minimum Volume of Specimen: Entire 24 hr collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 4-5 Days
Reference Range: See report
Catecholamines, plasma
General Use: Differential diagnosis of pheochromocytoma
Test Mnemonic: CATP
Test Includes: Dopamine, Epinephrine, Norepinephrine, and total catecholamines
(calculated)
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is required. Patient should avoid alcohol,
coffee, tea, tobacco and strenuous exercise prior to collection.
Special Instructions: Draw in pre-chilled tube. Place on ice; deliver to lab
immediately!
Specimen Required: Whole blood (plasma)
Container Required: 2 green top tubes (Na heparin) Draw in pre-chilled tubes,
place on ice and deliver ASAP.
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2.5 ml plasma
Causes for Rejection: Improper tube type, specimen storage or delay in transport
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Celiac DNA Genotyping
Synonyms: Celiac Disease HLA Typing
Test Mnemonic: CELDNA
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Do not spin!
Specimen Required: Whole blood (ACD-A or ACD-B)
Container Required: Yellow top ACD tube - obtain from Specimen Processing
Lab
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml
Availability: Sent Monday-Saturday (not holidays)
Reference Range: See report
Ceruloplasmin
General Use: Diagnostic test for Wilson's disease
Test Mnemonic: CERU
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Cholinesterase, Blood
Synonyms: Pseudo-Cholinesterase; Serum Cholinesterase
General Use: Evaluate prolonged anesthetic effect post surgery, nerve gas
poisoning or insecticide poisoning
Test Mnemonic: PSCHOL
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole Blood (EDTA)
Container Required: Lavender top tube with (EDTA)
Volume of Specimen: 4 ml
Minimum Volume of Specimen: 2 ml Plasma
Reasons for Rejection: Hemolysis
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See report
Chromium, Plasma
General Use: Occupational exposure and exposure to environmental
contamination of chromium may lead to toxicity.
Test Mnemonic: CHR
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should refrain from taking vitamins, mineral or
herbal supplements at least one week prior to specimen collection
Specimen Required: Navy EDTA Plasma
Container Required: Navy EDTA tube
Volume of Specimen: 4 ml
Minimum Volume of Specimen: 2 ml Plasma
Causes for Rejection: Clotted, grossly hemolyzed
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 1 Week
Reference Range: See report
Chromogranin A
Synonyms: CgA
General Use: Serum Chromogranin A is used to assess peptide-secreting
endocrine neoplasms and multiple endocrine neoplasia.
Test Mnemonic: CHROMA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, lipemia, icterus
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Chromosome Analysis, Tissue
Synonyms: Karyotype
General Use: Autosomal or sex chromosome mosaicism not detected in
lymphocytes may be determined in fibroblasts obtained from a tissue biopsy.
Test Mnemonic: TISCHR
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Tissue sample
Container Required: Transport Media
Volume of Specimen: 2x3 mm tissue sample
Minimum Volume of Specimen: 2x3 mm tissue sample
Causes for Rejection: Formalin Fixed
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 21 days
Reference Range: See individual reports
Citrate, 24 hour urine
Synonym: Citric Acid
General Use: Urine levels of Citrate are increased in metabolic and respiratory
alkalosis
Test Mnemonic: UCIT24
Test Includes: Creatinine, Citric acid
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form : Signed order by physician/Health Professional Affiliate(HPA
Patient Preparation: Call Specimen Processing lab for special collection
container
Special Instructions: Keep specimen refrigerated during entire collection
Specimen Required: 24 hr urine
Container Required: 24 hr urine container with no preservative, obtained from
Lab. Call ext 4153
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Chlamydia trachomatis DNA,SDA
Synonyms : CT, ProbeTec
General Use : For detection of C. Trachomatis
Test Mnemonic: CT
Lab Performing Test: Specimen processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional
Affiliate(HPA)
Specimen Required: Urine or endocervical,urethral or vaginal swab
Conatiner Required: BD Transport or BD Urine Preservative Transport
kit
Volume Of Specimen: One swab or 15-60 ml of urine
Minimum Volume of Specimen: One swab or 15 ml of urine
Causes for Rejection: Swabs received in any other transport kit.
Overfilled or underfilled urine transport container. Cleansing swab
received.
Availability: Sent out Monday –Saturday (not holidays)
Time Routinely Required for Processing: 4 days
Reference Range: Not detected
Clamydia trachomatis/Neisseria gonorrhoeae DNA, SDA
Synonym: CT/GC
General Use: For Detection of sexually transmitted diseases
Test Mnemonic: GCCAMP
Test Includes: Clamydia trachomatis, Neisseria gonorrhoeae
Lab Performing test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional
Affiliate (HPA)
Specimen Required: Urine or endocervical,urethral or vaginal swab
Container required: BD transport kit for swab or BD urine preservative
transport for urine
Volume of Specimen: One swab or 15-60 ml of urine
Minimum Volume of Specimen: One swab or 15 ml of urine
Causes for Rejection: Only BD collection kits allowed. Overfilled or
underfilled urine transport container. Collection kit received with
cleansing swab.
Availability: Sent out Monday-Saturday (not holidays)
Time Required Routinely for Processing: 4 days
Additional Information: Pink swab for vaginal specimens, blue swab for
urethral specimens
Reference Range: Not detected
Clonazepam
Synonym: Klonopin
General Use: Clonazepam is a benzodiazepine used as a tranquilizer.
Clonazepam is used in treating patients with seizures and in reducing tardive
dyskinesia.
Test Mnemonic: CLO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Optimum time to collect sample: 4 hours post oral dose
Special Instructions: Deliver ASAP!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gel barrier tube received
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Clozapine
Synonym: Clozaril
General Use: Clozapine is used selectively in the treatment of patients with
schizophrenia. Therapeutic drug monitoring is useful to optimize dose and to
avoid toxicity.
Test Mnemonic: CLOZ
Test Includes: Clozapine, Norclozapine
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
Specimen Required: EDTA plasma
Container Required: Purple top EDTA
Volume of Specimen: 4 mL
Minimum Volume of Specimen: 2 mL plasma
Causes for Rejection: Gross hemolysis, lipemia, received room temperature, gel
barrier tube received
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
CMV Antigenemia
Synonym: Cytomegalovirus Antigenemia
Test Mnemonic: CMVAG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver Immediately to Lab!
Specimen Required: Whole blood
Container Required: 2 Lavender (EDTA) top tubes
Volume of Specimen: 2 Lavender (EDTA) top tubes
Minimum Volume of Specimen: 2 Lavender (EDTA) top tubes
Causes for Rejection: Received room temperature
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Coagulation Factor Assays II,V,VII,IX,X,XI,XII
Synonyms: Factor Assays II,V,VII,IX,X,XI,XII
General Use: Detecting specific coagulation factor deficiencies
Test Mnemonic: FII; FV; FVII; FX; FXI; FXII
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Collection Instuctions: Centrifuge light blue-top tube for 15 minutes at
approximately 1500 g within 60 minutes of collection. Using a plastic pipette,
remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a
plastic vial. Centrifuge a second time and transfer platelet poor plasma into a new
plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately
and ship on dry ice.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate) – 2 for each test ordered
Volume of Specimen: 3 ml plasma for each test
Minimum Volume of Specimen: 1 ml plasma for each test
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See printed report
Cocaine Metabolites, Urine
Test Mnemonic: UCOCCO
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Urine
Container Required: Urine cup
Volume of Specimen: 20 mL
Minimum Volume of Specimen: 20 mL
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See printed report
Coccidioides Antibody
General Use: Aid in the diagnosis and prognosis of Coccidioides immitis
infections and in the planning of a fungus culture schedule
Test Mnemonic: COCC
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: This test is also available in the Fungal Serology Panel
Reference Range: Negative
Coenzyme Q10
Test Mnemonic: COEQ10
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should fast 10-12 hours prior to collection. Patient
may have water. It is not necessary to discontinue nutritional supplements prior to
this test.
Special Instructions: Protect from light; Deliver ASAP!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Received room temperature, not protected from light
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Cold Agglutinins
Synonyms: Cold Hemagglutinins
General Use: To aid in diagnosis of primary atypical pneumoniae and certain
hemolytic anemias
Test Mnemonic: CAG
Lab Performing Test: Specimen Processing – sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Blood, after being drawn, must be kept at room
temperature
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum. Blood > 1 hour old.
Specimen received frozen.
Availability: Sent Mon – Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See report
Collagen Type I C-Telopeptide (CTx)
Synonyms: C-Telopeptide, Serum, CTX, Beta-Crosslaps
General Use: CTx is useful to asses bone resorption in patients with metabolic
bone disease. The test is also useful in monitoring therapy to slow or halt
osteoporotic bone loss.
Test Mnemonic: CTELO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting required. Fasting morning collection 8-10 a.m.
(diurnal variations cause elevated levels at night).
Special Instructions: Allow blood to clot 10-15 minutes at room temperature.
Then centrifuge and separate serum from cells. Deliver to CP immediately!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Moderate/gross hemolysis, Grossly lipemic or icteric,
Non-fasting specimens
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Collagen Cross-Linked N-Telopeptide, Urine
Synonyms: N-Telopeptide, Osteomark, NTx
General Use: Screen and monitor osteoporosis
Test Mnemonic: UNTX
Test Includes: N-telopeptide, creatinine
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Random urine specimen should be the second void of the
morning
Specimen Required: Second morning urine void
Volume of Specimen: 25 ml urine
Minimum Volume of Specimen: 2 ml urine
Time Required Routinely for Processing: 5 days
Reference Range: See report
Complement, C2
Synonyms: C2
General Use: Complement levels aid in the diagnosis of certain inflammatory
conditions, autoimmune disease states, and some genetic disorders.
Test Mnemonic: COMPC2
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Separate serum within one hour of time draw and
refrigerate
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (No Gel!)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 8 days
Reference Range: See report
Complement, C5
Synonyms: C5
General Use: Complement levels aid in the diagnosis of certain inflammatory
conditions, autoimmune disease states, and some genetic disorders.
Test Mnemonic: COMPC5
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Complement, C6
Synonyms: C6
General Use: Complement levels aid in the diagnosis of certain inflammatory
conditions, autoimmune disease states, and some genetic disorders
Test Mnemonic: COMPC6
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 9 days
Reference Range: See report
Complement, Total (CH5O)
Synonyms: Total hemolytic complement
General Use: To follow SLE patient's response to therapeutic regime; to evaluate
for complement component deficiency
Test Mnemonic: CH5O
Lab Performing Test: Specimen Processing; Sent to Reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Special Instructions: Deliver to the lab immediately!
Container Required: Red top tube, no gel barrier!
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia, received room temperature,
delay in delivery to Lab
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Copper, 24 hour urine
Synonyms: Urine Cu
General Use: Evaluate disorders of copper metabolism, copper poisoning
Test Mnemonic: UCU24
Test Includes: Quantitative urine copper
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA).
Patient Preparation: Patient should refrain from taking vitamins, minerals or
herbal supplements at least 1 week prior to specimen collection
Special Instructions: Specimen must be voided into metal free container.
Refrigerate during collection
Specimen Required: 24 hour urine
Container Required: Acid washed 24 hour urine container supplied by
Specimen Processing Laboratory
Volume of Specimen: Entire collection
Availability: Sent Monday -Saturday (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See report
Copper, Blood
Synonyms: Cu
General Use: Evaluate abnormal copper metabolism; i.e., Wilson's Disease;
copper poisoning
Test Mnemonic: CU
Lab Performing Test: Specimen Processing; sent to reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood
Container Required: Navy blue top, (No additive)
Volume of Specimen: 7 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Hemolysis
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See report
Cortisol, Free 24 hour urine
General Use: The measurement of free cortisol in urine is a valuable tool for
diagnosis of Cushing’s Disease
Test Mnemonic: UCOR24
Test Includes: free cortisol and urine creatinine
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: 24 hour urine container supplied by Specimen Processing
Laboratory
Specimen Required: 24 hr urine
Container Required: 24 hr urine container
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 4-5 days
Reference Range: See Report
Coxsackie B Virus Titer
Synonyms: Coxsackie B 1-6
General Use: Used to support a diagnosis of Coxsackie virus infection
Test Mnemonic: COX
Test Includes: Coxsackie B Types 1, 2, 3, 4, 5, 6
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: Throat & rectal swabs will yield better viral recovery. It
is suggested that these accompany the serum sample.
Reference Range: Less than a four fold increase in titer
C-Peptide
Synonym: C-Terminal Insulin
General Use: Marker for endogenous insulin production
Test Mnemonic: CPEP
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should be fasting
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Room temperature specimens received more than 24 hous
after collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See report
Creatine Kinase Isoenzyme Panel
Synonyms: CPK Isoenzymes
Test Mnemonic: CPKISO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 mL
Minimum Volume of Specimen: 2 mL serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1-3 days
Additional Information: Use if presence of CKBB is needed for evaluation;
otherwise order "Heart" to obtain total CPK and CKMB
Reference Range: See report
Cryoglobulins
General Use: Detection of cold precipitating macroglobulins
Test Mnemonic: CRYOG
Lab Performing Test: Specimen Processing, sent to reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Draw in heated tube. Place tube in 37 degree heating block
immediately after specimen draw. After 1 hour in heating block, spin. Do not
refrigerate tube!
Specimen Required: Blood clotted at 37 degrees
Container Required: Heated plain red top tube (No Gel!) - 2
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2.5 ml serum
Causes for Rejection: Improper temperature storage
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 5 days.
Reference range: Negative
Cryptococcus Antibody
General Use: Aid in the diagnosis and prognosis of Cryptococcal infections and
in the planning of a fungus culture schedule
Test Mnemonic: CRY
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: This test is also available in the Fungal Serology Panel.
Reference Range: Negative
CSF IgG
Synonym: Immunoglobulin G, CSF
Test Mnemonic: CSFIGG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Cerebrospinal fluid (CSF)
Container Required: Sterile plastic tube
Volume of Specimen: 2 ml CSF
Minimum Volume of Specimen: 1 ml CSF
Causes for Rejection: Hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See Report
Cyclic Citrulline Peptide, IgG
Synonyms: Citrullinated Peptide (CCP) IgG, CCP, Anti-Cyclic Citrullinated
Peptide (CCP) Antibody IgG
General Use: This test has been proven to be better for use in the diagnosis of
Rheumatoid arthritis than conventional testing.
Test Mnemonic: CYCPE
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Cyclospora and Isospora Examination
General Use: Cyclospora cayetanensis and Isospora belli are parasites
that cause malaise, low grade fever and diarrhea.
Test mnemonic: CYCLSP
Test Includes: Cyclospora and Isospora Examination, Concentration
Lab Performing Test: Speciemn Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate
(HPA)
Specimen Required: Stool
Container Required:
Volume of Specimen:
Minimum Volume of Specimen:
Causes for Rejection: Unpreserved stool. Frozen specimens
Availability: Sent out Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See Report
Cyclosporine
General Use: Immunosuppressant Therapy
Test Mnemonic: CYA
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 5 mL
Minimum Volume of Specimen: 2 mL
Causes for Rejection: Improper tube type
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Cystic Fibrosis Screen
Synonym: Cystic Fibrosis Gene Mutation
General Use: Screening for relevant gene mutations
Test Mnemonic: CYSMUT
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: Lavender top tube (ETDA) - 2
Volume of Specimen: 2 lavender top tubes
Minimum Volume of Specimen: Entire collection
Causes for Rejection: Insufficient quantity
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Cystine, 24 hour urine
Test Mnemonic: UCYS24
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep refrigerated during collection. Deliver ASAP!
Specimen Required: 24 hour urine collection
Container Required: 24 hour urine container with no preservative
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Cytomegalovirus Antibodies
Synonyms: CMV
General Use: Used to support a diagnosis of Cytomegalovirus infection
Test Mnemonic: CMVAB
Test Includes: CMV IgG and IgM
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross Hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Cytomegalovirus Detection by PCR
General Use: Detection of CMV
Test Mnemonic: CMVPCR
Test Includes:
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool, urine, CSF, BAL, fluids or Tissue biopsy
Container Required: Fluids in sterile container. Tissue in viral transport media.
Volume Of Specimen:
Minimum Volume of Specimen:
Availability:
Time Required Routinely for Processing:
Additional Information:
Reference Range: See Report
Desipramine
Synonyms: Norpramin
General Use: Therapeutic drug monitoring
Test Mnemonic: DESI
Lab Performing Test: Specimen Processing - sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Collect as a trough or at least 12 hours after last dose
Special Instructions: Separate from cells as soon as possible
Specimen Required: Clotted blood (serum)
Container Required: Red top tube with NO gel barrier!
Volume of Specimen: 10 ml serum
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Drawn in tube with serum separator (SST), gross
hemolysis
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
DHEA Sulfate
General Use: DHEA-S is the sulfated form of DHEA and is the major androgen
produced by the adrenal glands. This test is used in the differential diagnosis of
hirsute or virilized female patients and for the diagnosis of isolated premature
adrenarche and adrenal tumors.
Test Mnemonic: DHEAS
Lab Performing Test: Specimen Processing - sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top SST tube
Volume of Specimen: 10 ml serum
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Diphtheria Antitoxoid Antibody
Synonym: Diphtheria Antibody IgG
General Use: Used to evaluate Diphtheria immunization efficacy.
Test Mnemonic: DIPAB
Lab Performing Test: Specimen Processing, sent to Reference lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Monday thru Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
DISOPYRAMIDE
Synonym: Norpace
General Use: Monitor therapeutic drug level
Test Mnemonic: NORPA
Test Includes: Disopyramide
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (No gel barrier)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Drawn in tube with gel barrier
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 5-6 days
Reference Range: See report
DNA (ds) Antibody
Synonyms: dsDNA, Double-Stranded DNA Antibody, Native DNA, Anti-DNA,
(dsDNA)
General Use: For diagnosis and follow up of SLE
Test Mnemonic: DSDNAB
Lab Performing Test: Specimen Processing; Sent to Reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, icterus, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Drug Screen, Serum Only
Test Mnemonic: DRGS
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Lab Performing Test: Specimen Processing - sent to reference lab
Specimen Required: Clotted blood (serum)
Container Required: Red top tube, No gel!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml serum
Causes for Rejection: Improper tube type
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See report
Echinococcus Serology
General Use: Used to support a diagnosis of Echinococcosus granulosus
Test Mnemonic: ECHIN
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4-8 days
Reference Range: Negative
Endomysial IgA Antibody
General Use: A positive IgA endomysial antibody result supports the diagnosis
of celiac disease.
Test Mnemonic: ENDMYS
Test Includes: If Endomysial Antibody Screen IgA is abnormal, Endomysial
Antibody Titer will be performed at an additional charge.
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Mon-Saturday (not holidays)
Causes for Rejection: Gross Hemolysis and lipemia
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Enterovirus Detection by PCR
General Use: Detection of Enterovirus
Test Mnemonic: ENTPCR
Test Includes:
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Throat swab, Nasopharyngeal swab or wash, stool and
others
Container Required: Viral Transport Media
Volume of Specimen:
Availability:
Time Required Routinely for Processing:
Additional Information:
Reference Range: See Report
Epstein-Barr Viral Panel
Synonyms: EBV Panel
General Use: Diagnosis and confirmation of Epstein-Barr Virus infection
Test Mnemonic: EBVP
Test Includes: Epstein-Barr Virus VCA Antibody (IgM), Epstein-Barr Virus
VCA Antibody (IgG), Epstein-Barr Virus Nuclear Antigen (EBNA) Antibody
(IgG)
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia, icterus
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Epstein-Barr Virus DNA, Quantitative PCR
Synonym: EBV Quant DNA
General Use: Epstein-Barr Virus DNA, Real-Time PCR is useful in assessing
active disease. Central nervous system infections can be diagnosed with CSF
specimens.
Test Mnemonic: EBVDNA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole Blood or plasma
Container Required: Lavender top (EDTA) tube
Volume of Specimen: 1 Lavender top (EDTA) tubes
Minimum Volume of Specimen: 1 ml whole blood or plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Erythropoietin
General Use: Diagnosis of kidney disease and anemia
Test Mnemonic: ERY
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Cause for Rejection: Gross hemolysis
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See report
Estrone, serum
General Use: Estrone is primarily derived from metabolism of androstenedione
in peripheral tissues, especially adipose tissues. Individuals with obesity have
increased conversion of androstenedione to Estrone leading to higher
concentrations. In addition, an increase in the ratio of Estrone to Estradiol may be
useful in assessing menopause in women.
Test Mnemonic: ESTRS
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes! Tube must be full!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 4 ml serum
Causes for Rejection: Gross hemolysis, lipemia, SST tube received
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3 days
Additional information: May also be ordered as part of serum estrogen panel,
which includes estradiol and estrone (mnemonic ESTROGS)
Reference Range: See Report
Extractable Nuclear Antigen Antibodies
Synonyms: Anti-Ribonucleic Protein Antigen, Anti-ENA Antibody, ENA
Antibodies, Anti-Smith Antibody, Ribonuclear Protein Antibody, RNP Antibody
General Use: Use to aid in the differentiation or diagnosis of SLE and Mixed
Connective Tissue Disease (MCTD)
Test Mnemonic: AENA
Test Includes: Anti-RNP and Anti-SM/RNP complex
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Cause for Rejection: Gross hemolysis, icterus, and/or lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Factor V Leiden Mutation Analysis
Synonyms: Factor Five
General Use: To determine the presence of a mutation in the Factor V molecule
producing a hypercoagulable state
Test Mnemonic: FVLEID
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: If specimen is drawn from an in-dwelling catheter, line
must be flushed with saline and the first 10 ml of blood collected must be
discarded.
Specimen Required: Whole blood at room temperature
Container Required: Lavender top tube (EDTA) - 2
Volume of Specimen: 2 lavender top tubes
Minimum Volume of Specimen: 1 lavender top tube
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Sent out Mon–Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See printed report
Factor VIII Multimers
Synonym: von Willebrand Factor Antigen, Multimeric
General Use: For the analysis of Von Willebrand Antigen multimers for the
diagnosis of Von Willebrands Disease.
Test Mnemonic: F8MULT
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting for at least 8 hours is preferred
Special Instructions: Deliver Immediately to Lab!
Specimen Required: Plasma
Container Required: Blue top tube (Sodium Citrate)
Volume of Specimen: Full blue top tube
Minimum Volume of Specimen: 2 ml plasma
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Factor VIII Related Antigen
Synonym: von Willebrand Factor Antigen
General Use: Interpreted by physician
Test Mnemonic: FVIIIA
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred. Patient must not be on
anticoagulants.
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Collection Instructions: Centrifuge light blue-top tube for 15 minutes at
approximately 1500 g within 60 minutes of collection. Using a plastic pipette,
remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a
plastic vial. Centrifuge a second time and transfer platelet poor plasma into a new
plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately
and ship on dry ice.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate) – tube must be full
Volume of Specimen: 2 ml plasma
Minimum Volume of Specimen: 1 ml plasma
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See printed report
Fecal Fat, Random
Synonyms: Qualitative stool for fat, lipids, stool
General Use: Screening test for presence of split fat globules and neutral fat
globules
Test Mnemonic: FFRAN
Lab Performing Test: Specimen Processing. Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Fresh random stool, no 24 hr collections or urines.
Container Required: Sterile cup, stool cup
Minimum Volume of Specimen: 2 grams
Causes for Rejection: Insufficient specimen volume, specimen contaminated
with urine and/or water, specimen containing interfering substances, eg. castor oil,
bismuth, metamucil, barium; no swabs accepted, no 72 hour fecal collection.
Availability: Sent Mon-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Fecal Fat, Timed specimen
Synonym: Lipids, Stool, Split Fats, Unesterfied Fatty Acids
General Use: Excessive Fat in stool is useful in diagnosing patients with
malabsorption and maldigestion, e.g., pancreatic failure. In addition, results may
be useful in monitoring patients receiving exogenous enzyme therapy for chronic
diarrhea.
Test Mnemonic: FF
Test Includes: Quantitative fecal fat
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should be on a diet including 100 grams of fat per
day for 3 days prior to collection and during the collection period.
Special Instructions: Collect in pre-weighed container, send entire collection.
Keep refrigerated during collection.
Specimen Required: Collect stool for 24, 48 or 72 hours
Container Required: Pre-weighed plastic container with tight lid
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 8-10 days
Reference Range: See Report
Flecainide
Synonym: Tambocor ®
General Use: Monitoring the flecainide concentration is used to assure
compliance and avoid toxicity of this cardiac drug used to treat ventricular
tachycardia and premature cardiac contractions.
Test Mnemonic: FLE
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Optimum time to collect sample: 1 hour before next dose.
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 4 ml serum
Causes for Rejection: SST tube received
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Flow Cytometry, Bone Marrow or Peripheral Blood
Synonyms: Leukemia/Lymphoma Panel, CLLP
Test Mnemonic: FLOW
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Clinical history required
Specimen Required: Whole blood
Container Required: 1 Green top tube (sodium heparin) or EDTA Lavender top
Volume of Specimen: 4.0 ml of bone marrow or whole blood
Minimum Volume of Specimen: 1.0 ml of bone marrow or whole blood
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Daily, Mon.-Fri.; not available on weekends; not done STAT
Time Required Routinely for Processing: 1-2 days
Reference Range: Interpretation made by pathologist
Folate, RBC
General Use: Detect folate deficiencies and monitor therapy
Test Mnemonic: FOLRBC
Lab Performing Test: Specimen Processing, sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep tube at room temp! Do not refrigerate or freeze.
Specimen Required: Whole blood, plasma
Container Required: Lavender top (EDTA) - 2
Volume of Specimen: 5 ml
Minimum Volume of Specimen: entire collection
Causes for Rejection: Improper storage temperature
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See Report
Fragile-X
Synonym: Fragile X with reflex
General Use: Routine chromosome analysis
Test Mnemonic: FRAGX
Test Includes: If Fragile X, PCR result is not Normal, or Gray zone, then Fragile
X, Southern Blot will be performed at an additional charge
Lab Performing Test: Specimen Processing. Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood - kept at room temp!
Container Required: 4 Lavender top tubes (EDTA)
Volume of Specimen: 10 ml whole blood
Minimum Volume of Specimen: 5.0 ml
Causes for Rejection: Improper tube type or specimen not kept at room temp
Availability: Sent Mon-Sat, (not holidays)
Time Required for Processing: 1 week
Reference Range: See report
Free T3
Synonym: FT3
General Use: Thyroid testing
Test Mnemonic: FT3
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube-(SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See Report
Free Testosterone
General Use: This test is helpful in the diagnosis of hypogonadism in males and
hirsutism and virilization in females
Test Mnemonic: FTEST
Test Includes: Testosterone, free, bioavailable, and total
Lab Performing Test: Specimen Processing - sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: 2 Red top tubes , No gel barrier tubes!
Volume of Specimen: 10 ml (2 tubes)
Minimum Volume of Specimen: 4 ml serum
Causes for Rejection: SST tube received
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See report
Fructosamine
General Use: Useful in monitoring the degree of glycemia over short to
intermediate time frames (1-3 weeks)
Test Mnemonic: FRUC
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Avoid hemolysis of specimen
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Hemolysis, icterus
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
FTA-CSF
Synonym: Treponema pallidum antibody
General Use: Test for neurosyphilis, The FTA-CSF test is considered
experimental at this time.
Test Mnemonic: FTASF
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Cerebrospinal fluid
Container Required: Clean sterile container, glass tube
Volume of Specimen: 2 ml of CSF
Minimum Volume of Specimen: 0.5 ml CSF
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: One week
Reference Range: Non-reactive
FTA Profile
General Use: Useful in the diagnosis and treatment of Syphilis
Test Mnemonic: FTAP
Test Includes: RPR Titer and FTA-ABS
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Fungal Serology Panel
General Use: Aid in the diagnosis and prognosis of fungal infection and in the
planning of a fungus culture schedule
Test Mnemonic: FUNGP
Test Includes: Aspergillus, Blastomyces. Coccidiomycosis, Histoplasmosis and
Cryptococcal Serologies
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: These tests may be ordered individually; see specific listing
for each fungus
Container Required: Red top tube (SST) – 2
Specimen Required: Clotted blood (serum)
Volume of Specimen: Two 10 ml tubes
Minimum Volume of Specimen: 5 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Gabapentin
Synonym: Neurontin
General Use: Gabapentin is an anticonvulsant drug commonly used as adjunctive
therapy to treat partial seizures.
Test Mnemonic: GABAP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Draw sample 2 hours after last dose at steady state.
Special Instructions: Separate plasma from cells ASAP!
Specimen Required: Plasma
Container Required: Lavender top (EDTA) tube - 2
Volume of Specimen: 3 ml plasma
Availability: Sent out Mon-Sat (not holidays)
Causes for Rejection: SST tube received, grossly hemolyzed
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Ganglioside GM-1 Antibodies
Synonym: GMI Antibodies
General Use: Ganglioside GM-1 Antibody IgG is associated with the GuillainBarre syndrome, particularly the acute motor axonal neuropathy variant. Antibody
IgM is associated with chronic multifocal motor neuropathy.
Test Mnemonic: GANGAB
Test Includes: GMI IgG and IgM antibodies
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume Required: 2 ml serum
Availability: Sent Monday-Friday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Gastric Parietal Cell Antibody
General Use: 80% of patients with pernicious anemia have Anti-parietal cells Ab.
Useful in diagnosis of atrophic gastritis
Test Mnemonic: GPCAB
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Gastrin
Synonyms: Serum Gastrin
General Use: Diagnosis of Zollinger-Ellison syndrome and of pernicious anemia
Test Mnemonic: GASTR
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient fasting overnight or at least 12 hours before
specimen is drawn
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST); Deliver immediately to lab
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2.0 ml serum
Causes for Rejection: Gross hemolysis, lipemia, icterus
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Glucagon
General Use: Glucagon measurement is useful primarily when considering a
glucagon-secreting tumor of the pancreas. Glucagon is also used to diagnose
glucagon deficiency in patients with hypoglycemia.
Test Mnemonic: GLUCA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is required
Specimen Required: plasma
Container Required: 2 Lavender top (EDTA) tube
Volume of Specimen: 2 Lavender top (EDTA) tubes
Minimum Volume of Specimen: 3 ml plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Glucose-6-Phosphate Dehydrogenase
Synonym: G-6-PD
General Use: Confirmatory test to determine the cause of hemolysis
Test Mnemonic: G6PHD
Lab Performing Test: Specimen Processing, sent to Reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 5.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 1.0 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent Mon-Sat, no holidays
Time required for Processing: 3-5 days
Reference Range: See Report
Glutamic Acid Decarboxylase-65 Antibody
Synonym: Anti-GAD
General Use: Useful in identifying Type 2 diabetic individuals who may progress
to Type 1
Test Mnemonic: GGAD65
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume Required: 1 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Growth Hormone (HGH), Human
Synonyms: Human Growth Hormone (HGH); HGH;
General Use: (1) Diagnosis of hypopituitarism (2) Diagnosis of acromegaly
Test Mnemonic: HGRH
Lab Performing Test: Specimen Processing: Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Random growth hormone collection should be performed
on fasting patients who have rested for at least 30 minutes prior to collection
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, Plasma
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Haptoglobin
General Use: Evaluation of intravascular hemolysis and acute or chronic
inflammatory response
Test Mnemonic: HAP
Lab Performing Test: Specimen Processing; Sent to reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml serum
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3-4 days
Reference Range: See report
Heavy Metal Screen, blood
General Use: Useful in the diagnosis of toxicity due to arsenic, cadmium, lead or
mercury exposure
Test Mnemonic: HEAVY
Test Includes: Arsenic, Lead, Cadmium and Mercury
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should refrain from eating seafood and taking
herbal supplements for at least 3 days before specimen collection.
Specimen Required: Whole blood
Container Required: 3 Navy top (EDTA) tubes and 1 Tan top (K-EDTA or
sodium heparin) tube
Volume of Specimen: 3 - 10 ml Navy (EDTA) tubes and 1- 5 ml Tan tube
Minimum Volume of Specimen: 3 Navy (EDTA) tubes and 1 Tan tube
Causes for Rejection: Improper tube types
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Helicobacter Antibodies
General Use: To aid in the diagnosis of infection with Helicobacter sp or as a
marker of bacterial eradication following therapy
Test Mnemonic: HELI
Test Includes: Helicobacter IgG, IgM and IgA
Lab Performing Test: Specimen Processing; Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Hemoglobin Electrophoresis
Synonym: Hemoglobinopathy Evaluation
General Use: To rule out the presence of abnormal hemoglobin
Test Mnemonic: HGBELE
Test Includes: Hemoglobin A1, Fetal Hemoglobin, Hemoglobin A2 and any
hemoglobin variants, Red Blood Cell Count, Hemoglobin, Hematocrit, MCV,
MCH, RDW
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4 ml whole blood
Minimum Volume of Specimen: 3 ml of whole blood
Availability: Sent out Monday - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Heparin Anti-Xa LMWH Assay
Synonyms: Anti-Xa Assay
General Use: To determine therapeutic levels of low molecular weight heparin
Test Mnemonic: XAHEP
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should be on low molecular weight heparin
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Collection Instructions: Deliver to lab immediately!
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2 ml plasma
Minimum Volume of Specimen: 1 ml plasma
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See printed report
Heparin Induced Platelet Antibody
Synonym: Heparin Associated Platlet Antibody
General Use: Heparin can induce thrombocytopenia, a decreased platelet count,
due to antibody stimulation. False positive results may be due to immune
complexes. False negative results may be due to low titer, low avidity antibodies.
Test Mnemonic: HIPAB
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Separate serum from cells ASAP! Deliver to lab ASAP
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Hepatitis A Antibody, Total with Reflex to IgM
General Use: To confirm acute or convalescent phase of Hepatitis A infection.
Test Mnemonic: HEPATO
Test Includes: If Hepatitis A Antibody Total is reactive, Hepatitis A IgM
Antibody will be performed at an additional charge
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Hepatitis B E Antibody
General Use: Presence of antibody is prognostic for resolution of infection due to
Hepatitis B virus
Test Mnemonic: HBENAB
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Reported as positive or negative
Hepatitis B E Antigen
General Use: Predictor of acute active infection with Hepatitis B virus
representing the most infectious period.
Test Mnemonic: HBENAG
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Hepatitis B Surface Antigen, Neutralization
General Use: Surface antigen usually appears in the serum after an incubation
period of 1 to 6 months following exposure to Hepatitis B virus and peaks shortly
after onset of symptoms. It typically disappears within 1 to 3 months. Persistence
of Hepatitis B surface antigen for greater than 6 months is a prognostic indicator
of chronic Hepatitis B infection.
Test Mnemonic: HBSAGN
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Gross hemolysis and lipemia
Availability: Sent out Mon - Sat (not holidays)
Reference Range: See Report
Hepatitis B Viral (HBV) DNA Quantitative by PCR
General Use: Chronic carriers will persist in producing detectable HBV. Patients
with chronic liver disease of unknown origin most commonly have HBV that is
detected by viral DNA testing. Quantitative measurement of HBV viral DNA may
be used to monitor progression of disease.
Test Mnemonic: HBVDNA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
Specimen Required: Plasma
Container Required: 2 lavender top EDTA tubes
Volume of Specimen: 3 mL plasma
Minimum Volume of Specimen: 2 ml plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 8 days
Reference Range: See Report
Hepatitis C Genotype
General Use: Genotypes are useful as a predictor of the efficacy of anti-viral
therapy and disease management
Test Mnemonic: HCGENO
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: 2 lavender top EDTA tubes; Deliver blood immediately!
Volume of Specimen: 2 ml plasma
Minimum Volume of Specimen: 1 ml plasma
Cause for Rejection: Improper tube type
Availability: Sent Mon.- Sat. (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Hepatitis C RNA
General Use: To quantitate viral load in patients with chronic Hepatitis C or to
measure response to alpha interferon treatment
Test Mnemonic: HCVRNA
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver tubes immediately!
Specimen Required: Whole blood (EDTA)
Container Required: 2 lavender top tubes, Deliver immediately!
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 3 mL plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Hereditary Hemochromatosis, DNA, Mutation Analysis
Synonym: Hemochrom HFE Gene Anal, Blood, Hemochromatosis
General Use: Hereditary Hemochromatosis (HH) is an inherited disorder wherein
the body accumulates excess iron. This test establishes HH diagnosis in
individuals with abnormal iron study results and identifies at-risk family
members.
Test Mnemonic: HEMOCH
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: whole blood
Container Required: lavender top (EDTA)
Volume of Specimen: 2 lavender top tubes (5ml)
Minimum Volume of Specimen: entire collection
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Herpes Simplex Virus Antibodies
Synonyms: Herpes simplex titers
General Use: Used to support a diagnosis of Herpes Virus infection
Test Mnemonic: HERPE
Test Includes: Herpes Abs IgG and IgM, Types I & II
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Herpes Simplex Virus Culture with Reflex Typing
Synonym: Herpes Simplex Virus Culture, includes Typing
General Use: There are two HSV serotypes. HSV 1 is commonly associated
with infection of non-genital sites. HSV 2 is most commonly associated with
genital tract and neonatal infections. Typing may assist in prognosis for lesion
recurrence and epidemiological studies.
Test Mnemonic: HERP
Test Includes: If culture is positive, typing is performed at additional charge
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Vesicle,fluid, scrapings or swab from lesion,. 2 ml CSF
urogenital and respiratory specimens, conjuntival or corneal scrapings or tissues
in sterile leak-proof container
Container Required: V-C-M medium (green cap) tube
Volume of Specimen: One swab or 2ml CSF
Minimum Volume of Specimen: One swab or 1ml of CSF
Special Instructions: Use only plastic shaft rayon on Dacron swabs
Causes for Rejection: Raw stool, dry swabs, gel-based transport systems, tissues
in fixatives, sputum, specimens received frozen and wooden shaft swabs
Availability: Mon- Fri 7am to 3pm
Time Required Routinely for Processing: 2 weeks
Reference Range: See Report
Herpes Simplex Virus (HSV) IgG 1&2
General Use: This assay is type specific and will differentiate between HSV-1
and HSV-2 infections
Test Mnemonic: HSVG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Additional Information: May also be ordered as part of the panel HERPE,
which includes HSV IgG 1&2 and HSV IgM
Reference Range: See Report
Herpes Simplex Virus (HSV) IgM Antibody with Reflex to Titer
General Use: To distinguish a primary from a recurrent HSV infection. However,
in case of extensive infection (recurrent), an IgM response may be observed.
Test Mnemonic: HERIGM
Test Includes: If HSV IgM is positive, a titer will be performed at an additional
charge.
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross lipemia
Availability: Sent out Mon-Sat (not holidays)
Additional Information: May also be ordered as part of the panel HERPE,
which includes HSV IgG 1&2 and HSV IgM
Reference Range: See Report
Herpes Simplex Virus/Varicella-Zoster Virus, Rapid Culture
General Use: To distinguish VZV from HSV
Test Mnemonic: HSVVZV
Test Includes: HSV rapid culture and VZV rapid culture
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Swab of lesion or aspirate from mouth, skin or
conjunctiva, respiratory specimens, tissue sections, body fluids or CSF
Conatiner Required: V-C-M medium (green cap) tube
Volume of Specimen: One swab or 0.3 ml aspirate or body fluid
Minimum Volume of Specimen: One swab or 0.3 ml aspirate or body fluid
Additional Information: Genital, rectal and peri-rectal specimen should be
ordered as HERP
Causes for Rejection: Dry swabs, gel-based transport systems,slides, stool,
urines or wooden-shaft swabs
Availability: Mon – Fri 7am to 3pm
Time Required Routinely for Processing: 5 days
Reference Range: See Report
Herpes Virus Detection by Direct Fluorescent Antibody (DFA) Stain and Culture
General Use: For Detection of Herpes virus
Test Mnemonic: HERPDFA
Test Includes:
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required:
Container Required: Viral transport media
Volume of Specimen:
Minimum Volume of Specimen:
Causes for Rejection:
Availability:
Time Required Routinely for Processing:
Reference Range: See Report
Herpes Virus Detection by PCR
General Use: Detection of Herpes Virus
Test Mnemonic: HERPPCR
Test Includes:
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional
Affiliate(HPA)
Specimen Required: CSF best specimen to test
Container Required: Sterile Tube
Volume of Specimen:
Minimum V olume of Specimen:
Additional Information: Newborns are swabbed on 4 sies with one swab
(conjunctiva,mouth,NP,rectum)
Causes for Rejection:
Availability:
Time Required Routinely for Processing:
Reference Range: See Report
Histamine, 24 hour urine
General Use: Histamine is a mediator of the allergic response. Histamine release
causes itching, flushing, hives, vomiting, syncope, and even shock. In addition,
some patients with gastric carcinoids may exhibit high concentrations of
histamine.
Test Mnemonic: UHIS24
Test Includes: Creatinine
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should avoid direct sunlight. Patient should avoid
taking antihistamines, oral corticosteroids and substances which block H2
receptors for at least 24 hours prior to specimen collection.
Specimen Required: 24 hour urine collection
Container Required: 24 hour urine container with 10 ml 6N HCl, supplied by
Specimen Processing lab
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Histamine, blood
General Use: Histamine is a mediator of the allergic response. Histamine release
causes itching, flushing, hives, vomiting, syncope, and even shock. In addition,
some patients with gastric carcinoids may exhibit high concentrations of
histamine.
Test Mnemonic: HISTA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Avoid taking allergy causing drugs, antihistamines, oral
corticosteroids and substances which block H2 receptors 24 hours prior to
collection.
Special Instructions: Deliver ASAP!
Specimen Required: Plasma
Container Required: Lavender top (EDTA) tube
Volume of Specimen: 1 Lavender top (EDTA) tube
Minimum Volume of Specimen: 1 mL plasma
Causes for Rejection: Hemolysis, lipemia, received room temperature
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Histone Antibodies
Synonyms: Anti-Histone Antibody
General Use: Diagnostic for differentiating drug-induced lupus from SLE
Test Mnemonic: HISTAB
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Histoplasma Antibody
General Use: Aid in the diagnosis of acute broncho-pulmonary histoplasmosis
and in the planning of a fungus culture schedule.
Test Mnemonic: HIS
Test Includes: Antibodies to Yeast and Mycelial antigens
Lab Performing Test: Specimen Processing; Sent to Reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: This test is also available in the Fungal Serology Panel
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Histoplasma Galactomannan Antigen, Urine
General Use: Frequently detected in urine of patients with disseminated
histoplasmosis.
Test Mnemonic: HISTUR
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: urine in sterile container
Container Required: Sterile leak-proof urine cup
Volume Of Specimen: 10 ml random urine
Minimum Volume of Specimen: 1 ml random urine
Availability: Mon –Fri 7am to 3pm
Time Required Routinely for Processing: 3-6 days
References Range: See Report
HIV,DNA by PCR
General Use: To aid in the diagnosis of AIDS
Test Mnemonic: HIVDNA
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Blood must stay at room temp until reaching Lab!
Specimen Required: Whole blood (EDTA)
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 3 ml whole blood
Availability: Sent out Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Not detected
HIV, RNA by PCR (Ultra)
General Use: Viral load testing, quantitative
Test Mnemonic: HIVQ
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver to lab immediately!
Specimen Required: Plasma
Container Required: Lavender top (EDTA) - 2
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 3 mL plasma
Causes for Rejection: Delay in transport
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
HIV Genotype
General Use: Evaluation of HIV drug resistance
Test Mnemonic: HIVGEN
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Special Instructions: Deliver tubes to lab immediately!
Container Required: Two Lavender top tubes (EDTA)
Volume of Specimen: Two 4 ml tubes
Minimum Volume of Specimen: 3 ml plasma
Causes for Rejection: Improper tube type, delay in receipt of specimen
Availability: Sent Mon.-Sat. (not holidays)
Reference Range: See report
HIV-1 Phenotype
Synonym: HIV Antivirogram
General Use: Resistance testing is useful when HIV treatment is initiated for
changed.
Test Mnemonic: HIVPHE
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (plasma)
Container Required: 2 Lavender top EDTA tubes – deliver to Lab immediately
Volume of Specimen: 2- 4ml tubes
Minimum Volume of Specimen: 2 ml plasma
Causes for Rejection: Delay in transport of specimen, improper tube type
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 10 days
Additional Information: Assay is intended for use only for patients with viral
loads of ≥500 copies.
Reference Range: See Report
HLA - B27
Synonyms: HLA - Antigen; B27; Tissue Typing
General Use: Differential diagnosis of arthritis, HLA-B27 is a marker for MarieStrumpel Disease and may be associated with other diseases
Test Mnemonic: HLAB27
Lab Performing Test: Specimen Processing
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: 10 mL green top- Na Hep. Obtain from Specimen
Processing Lab
Volume of Specimen: 10 ml. Keep at room temp. Deliver Immediately!
Minimum Volume of Specimen: 5 ml
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
HTLV ABS I & II
Test Mnemonic: HTLVAB
Test Includes: If HTLV-I/II Antibody is positive, HTLV-I/II Antibody,
Confirmatory Assay will be performed at an additional charge
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Centrifuge specimen ASAP
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Hypersensitivity Pneumonitis Evaluation
General Use: Aid in diagnosis of hypersensitivity pneumonitis
Test Mnemonic: HYPEV
Test Includes: Micropolyspora faeni, Saccharomonospora viridis, T. candidus,
T. sacchari, T. vulgaris, Aspergillus fumigatus, A. pullulans IgG, Alternaria
tenuis IgG, Cladosporium herbarum IgG, Penicillium notatum IgG, Phoma spp
IgG, Trichoderma viride IgG, Mixed Feathers IgG, Pigeon Droppings IgG
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 9 days
Reference Range: See report
Hypersensitivity Pneumonitis Screen
Synonyms: Aspergillus precipitans, Hypersensitivity screen
General Use: Aid in diagnosis of hypersensitivity pneumonitis
Test Mnemonic: HPS
Test Includes: Aspergillus fumigatus, Micropolyspora faeni, Pigeon Serum, T.
candidus, T. vulgaris, S. viridis
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
IDPH see Immune Deficiency Panel
IGF (Insulin-like growth factor) Binding Protein-2
Test Mnemonic: IGFP2
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
IGF (Insulin-like growth factor) Binding Protein-3
General Use: Of the six distinct IGF binding proteins structurally characterized at
this time, IGFBP-3 has been shown to be the major carrier of the IGFs,
transporting approximately 95% of circulating IGF-I and IGF-II.
Test Mnemonic: IGFBP3
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
IgG (Immunoglobulin G) Subclass 4
Test Mnemonic: IGGSC4
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis; lipemia
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
IgG Subclass Fractionation
Synonym: Immunoglobulin IgG subclasses
General Use: Aid in diagnosis of primary Immunodeficiencies, AIDS, Recurred
infectious Diseases, Parasitic Disease, Allergic Disorders, Autoimmune Disease,
Malignancies
Test Mnemonic: IGGSUB
Test Includes: IgG1, IgG2, IgG3, IgG4, total IgG
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting (overnight) preferred
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent Monday - Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Imipramine
Synonyms: Tofranil
General Use: Therapeutic drug monitoring.
Test Mnemonic: IMIP
Test Includes: Desipramine (Metabolite)
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube with NO gel barrier!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Drawn in tube with serum separator (SST), Gross
hemolysis
Availability: Sent out Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See report
Immune Complex, C1 Q
General Use: High values are associated with the presence of circulating
immunecomplexes (CIC).
Test Mnemonic: C1Q
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver to Lab immediately!
Specimen Required: Clotted Blood (serum) No Gel barrier tube!
Container Required: Red top tube (no gel barrier!)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Moderate and gross hemolysis, lipemia, plasma
Availability: Sent out Mon-Sat (not holidays)
Additional Information: May also be ordered as part of an Immune Complex
Panel
Reference Range: See report
Immune Complex Panel
General Use: To demonstrate the formation of circulating immune complexes
Test Mnemonic: IMCP
Test Includes: CIC-C3d IgG (Raji) and Immune Complex C1Q
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (No gel barrier!) Deliver immediately to Lab!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Immune Deficiency Panel
Synonyms: T & B Cells, Lymphocyte Subset Panel 3
General Use: Used in assessment of immune deficiency disease (AIDS) i.e. HIV.
Test Includes: Absolute Lymphocytes, Percentage CD3, Absolute
CD3, Percentage CD4, Absolute CD4, Percentage CD8, Absolute CD8, CD4/CD8
Ratio (calculated)
Test Mnemonic: LYMSP3
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Must be kept at room temperature - do not refrigerate. If a
CBC is also required, a separate EDTA (lavender-top) tube must be submitted.
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 4.0 ml
Causes for Rejection: Clotted, hemolyzed, or refrigerated specimen, incorrect
specimen volume, mislabeled or unlabeled specimen.
Availability: Monday through Friday (cut off for drawing on Friday is 2 pm, no
Sat or Sun draws)
Time Required Routinely for Processing: 2 - 3 days
Reference Range: See printed report
Immunoelectrophoresis, Serum
Synonyms: IEP
Test Mnemonic: IEPS
Test Includes: Immunoglobulins, Immunofixation and Serum Protein
Electrophoresis (SPE)
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Hemolysis
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: Includes Immunofixation and Protein electrophoresis
Reference Range: See report
Immunoglobulin D
Synonyms: IgD
General Use: Useful in the diagnosis of myeloma, chronic infections, and
acquired deficiency disorders
Test Mnemonic: IGD
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Influenza Antibody Panel
General Use: Used to support a diagnosis of influenza virus infection
Test Mnemonic: INFLAB
Test Includes: Influenzae A and B
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Insulin Antibody (Insulin Auto-antibody)
General Use: Present in 50% of newly diagnosed Type I diabetic and individuals
with Insulin resistance
Test Mnemonic: INSAB
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (no gel barrier!)
Volume of Specimen: 10 ml
Minimum Volume Required: 1 ml
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Insulin Growth Factor-1
Synonym Somatomedin-C, IGF-1
General Use: Diagnosis of acromegaly and pituitary deficiency
Test Mnemonic: IGF1C
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red – no gel barrier! Deliver to Lab ASAP!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 8 days
Reference Range: See Report
Intrinsic factor Antibody
Synonyms: IF-blocking Antibodies
General Use: Diagnostic of pernicious anemia.
Test Mnemonic: IFAB
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Islet Cell Antibody
General Use: Useful as an early marker of beta pancreatic cell destruction
Test Mnemonic: ISLET
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
JAK2 Mutation
General Use: Diagnose Polycythemia Vera (PV), Essential Thrombocythemia
(ET), and Idiopathic Myelofibrosis (MF)
Test Mnemonic: JAK2
Test Includes: If the JAK2 V617F result is negative, then Exons 12, 13 will be
performed at an additional charge
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Label tube with time and date of draw
Specimen Required: Whole Blood (EDTA)
Container Required: Lavender top EDTA
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 4 ml
Causes for Rejection: Gross hemolysis, clotted blood
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See report
Jo-1 Antibody
General Use: To aid in the diagnosis of polymyositis and dermatomyositis
Test Mnemonic: JO1
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Sent out Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Keppra
Synonym: Levetiracetam
General Use: Monitoring for patients on Keppra therapy
Test Mnemonic: KEPPRA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tubes!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Received room temperature, incorrect tube
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Lamictal
Synonym: Lamotrigine
General Use: Drug monitoring for patients on Lamotrigine therapy
Test Mnemonic: LAMIC
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube, No gel barrier! Deliver to Lab immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Causes for Rejection: Improper tube type, gross hemolysis, lipemia
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Lead, Blood
Synonyms: Pb, Blood
General Use: Lead toxicity, lead poisoning
Test Mnemonic: LEADP
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole venous blood
Container Required: 1 Lavender top( Must be protected from light) tube and 1
tan top tube
Volume of Specimen: Lavender top 5 ml (protect tube from light!), Tan top 3 ml
Minimum Volume of Specimen: 3 ml each tube
Causes for Rejection: Lavender top tube not protected from light
Availability: Sent Monday-Friday (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: For STAT Lead testing contact Specimen Processing to
make special arrangements.
Reference Range: See report
Legionella Serology
Synonyms: Legionnaires Disease Serology
General Use: To support diagnosis of infection with Legionella pneumophila
Test Mnemonic: LEGS
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (No Gel)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Lipoprotein A
Synonym: Lp(a)
General Use: Elevations of Lpa are associated with increased risk of CAD
Test Mnemonic: LPA
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must be fasting
Specimen Required: Clotted blood (serum)
Container Required: Red top tube- (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross lipemia
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Lipoprotein Fractionation Panel 2, Ion Mobility
General Use: Evaluation of hyper or hypolipidemia; phenotyping
Test Mnemonic: LIPOSZ
Test Includes: LDL, Total; LDL, Medium and Small; LDL, Very Small; HDL,
Large; LDL Peak Diameter; LDL Phenotype
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: 12-14 hours fasting required
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Non-fasting sample, gross hemolysis, lipemia
Availability: Sent Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report for interpretation
Lipoprotein Electrophoresis
Test Mnemonic: LIPOEL
General Use: Evaluation of hyper or hypolipidemia; phenotyping
Test Includes: Cholesterol, Triglycerides, Chylomicrons, Beta Lipoproteins, PreBeta Lipoproteins, and Alpha Lipoproteins
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: 12-14 hours fasting required
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (No Gel)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Non-fasting sample
Availability: Sent Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report for interpretation
Liver-Kidney Microsomal Antibody (IgG)
Synonyms: Anti -LKM
General Use: Anti-LKM Abs are found in a subgroup of patients with
autoimmune chronic active hepatitis (CAH)
Test Mnemonic: LKM
Lab Performing Test: Specimen Processing; Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Reasons for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Lorazepam
Synonym: Ativan
Test Mnemonic: LORAZ
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: 2 Red top tubes – No gel tubes!
Volume of Specimen: 2 Red top (no gel) tubes
Minimum Volume of Specimen: 4 ml serum
Causes for Rejection: SST Tube
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See Report
Lyme Disease Antibody with Reflex to CSF ratio
General Use: The Borrelia burgdorferi antibody index is used as an aid for the
diagnosis of neuroborreliosis.
Test Mnemonic: LYMIND
Test Includes: Borrelia burgdorferi IgG, IgM Antibodies (CSF and Serum); Total
IgG and Total IgM (CSF and Serum); Albumin (CSF and Serum); Borrelia
burgdorferi Antibody Index; Albumin Ratio
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Test requires both serum and CSF. Collect serum and CSF
samples within 24 hours of each other.
Specimen Required: CSF and Clotted blood (serum)
Container Required: CSF – sterile tube; Red top tube (SST)
Volume of Specimen: 2 ml CSF and 1 Red top tube (SST)
Causes for Rejection: For CSF collection, it is important to obtain a clean tap.
The presence of red cells invalidates the assay. Xanthrochromic or turbid CSF is
not acceptable.
Availability: sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 4-8 days
Reference Range: See Report
Lyme Titer, CSF
General Use: To aid in the diagnosis of CNS involvement with Lyme disease
Test Mnemonic: LYMESF
Test Includes: Lyme IgG and Igm titers
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: It is recommended that serum samples be ordered along
with CSF tests for valid comparison and interpretation
Specimen Required: Cerebrospinal fluid
Container Required: Sterile container, glass or plastic tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 1 ml
Availability: Sent Mon.-Sat., not holidays
Time Required Routinely for Processing: 1 week
Reference Range: See report
Lyme Titer, Misc Fluid
General Use: To aid in diagnosis of Lyme arthritis with joint involvement
Test Mnemonic: LYMISC
Test Includes: Lyme fluid IgG and IgM
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Please specify fluid source
Specimen Required: Appropriate fluid (not CSF)
Container Required: Sterile glass or plastic tube
Volume of Specimen: 2 ml fluid
Minimum Volume of Specimen: 1 ml fluid
Causes for Rejection: Clotted fluid sample
Availability: Sent Mon.-Sat., not holidays
Time Required Routinely for Processing: 1 week
Reference Range: See report
Lyme - Western blot
Synonyms: Lyme, Immunoblot, Burrelia burgdorferi
General Use: To support a diagnosis of Lyme disease or to rule out "false
positive" ELISA tests
Test Mnemonic: WESTQ
Test Includes: Antibody banding to IgG and IgM
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: No antibody bands present. Interpretation provided with report
Lymphocyte Enumeration Panel
Synonym: Lymphocyte Subset Panel 1
General Use: Immunophenotypic analysis may assist in evaluating cellular
immunocompetency in suspected cases of primary and secondary
immunodeficiency states.
Test Mnemonic: LENUP
Test Includes: Absolute Lymphocytes, Absolute CD3, Percentage CD3, Absolute
CD4, Percentage CD4, Absolute CD8, Percentage CD8, CD4/CD8 Ratio
(calculated), Absolute CD16/56, Percentage CD16/56, Absolute CD19,
Percentage CD19
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep at Room Temperature! If a CBC is also required, a
separate EDTA (lavender-top) tube must be submitted.
Specimen Required: Whole blood
Container Required: 3 Lavender top (EDTA) tubes
Minimum Volume of Specimen: 3 Lavender top tubes
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Lymphocytic Choriomeningitis Virus Antibody
Synonyms: LCM Ab
General Use: To aid in the diagnosis of infection due to LCV
Test Mnemonic: LCV
Test Includes: Lymphocytic Choriomeningitis Virus Antibodies IgG and IgM
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Lysozyme
Synonym: Muramidase
General Use: Lysozyme plays an important role in a host's defense against
microorganisms. Lysozyme concentrations are increased in patients with acute
myelocytic leukemia with monocytic differentiation (FAB M4/M5).
Concentrations may also be increased in patients with other leukemias,
sarcoidosis, and infections such as tuberculosis. With sarcoidosis, lysozyme may
be useful in monitoring disease and treatment.
Test Mnemonic: LYSO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Reasons for Rejections: Incorrect tube type
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Magnesium, RBC
General Use: Magnesium is an essential trace element. Deficiency leads to
irritability, neuromuscular abnormalities, cardiac and renal damage. Its salts are
used as antacids and cathartics. Excessive amount may cause CNS depression,
loss of muscle tone, respiratory and cardiac arrest.
Test Mnemonic: MAGRBC
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Pateint should refrain from taking vitamins or mineral
herbal supplements for at least one week before sample collection
Specimen Required: Whole Blood (EDTA)
Container Required: Lavender top EDTA tube
Volume of Specimen: Entire specimen
Minimum Volume of Specimen: 1 mL whole blood
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Magnesium, Urine 24 hour
General Use: Evaluate magnesium metabolism
Test Mnemonic: UMG24
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: 24 hour collection or random urines
Patient Preparation: Do not include first morning specimen. Collect all
sunsequent voiding. The last sample should be the first morning specimen voided
the following morning at the same time as the previous morning’s first voiding.
Container Required: 24 hour acidified urine container obtained from Specimen
Processing Lab
Volume of Specimen: Entire specimen
Minimum Volume of Specimen: Entire specimen
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Magnesium, Urine Random
General Use: Evaluate magnesium metabolism
Test Mnemonic: UMG
Test Includes: Creatinine
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Random urine
Container Required: No preservative!
Volume of Specimen: Entire specimen
Minimum Volume of Specimen: 1 mL urine
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Mephobarital Level
Synonyms: Mebaral
General Use: Monitor therapeutic drug level
Test Mnemonic: MEPBAR
Test Includes: Mephobarbital and Phenobarbital (metabolite)
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube - No gel barrier!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Reasons for Rejection: Collected in SST tube
Availability: Sent Mon-Sat. (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
MecSTAT 7 panel
General Use: Drug screening for breast milk
Test Mnemonic: MEC7
Test Includes: 7 drug panel
Lab Performing Test: Specimen Processing, sent to Reference lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Requires “Chain of Custody”. Call Specimen Processing
lab for instructions @ ext 4153
Specimen Required: Breast Milk
Container Required: special tamper proof container, call Specimen Processing
lab for kit.
Minimum Volume of Specimen: 10 mLs of breast milk
Causes for Rejection: Chain of custody protocol not followed or insufficient
sample volume.
Availability: Sent Mon-Sunday including holidays
Time Required Routinely for Processing: 1-3 days
Reference Range: See Report
MecSTAT 9 panel
General Use: Drug screening for newborns
Test Mnemonic: MEC9
Test Includes: 9 drug panel
Lab Performing Test: Specimen Processing, sent to Reference lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Requires “Chain of Custody”. Call Specimen Processing
lab for instructions @ ext 4153
Specimen Required: Meconium
Container Required: Special tamper proof container, call Specimen Processing
lab for kit.
Minimum Volume of Specimen: 2 gms of meconium
Causes for Rejection: Chain of custody protocol not followed or insufficient
sample volume.
Availability: Sent Mon-Sunday including holidays
Time Required Routinely for Processing: 1-3 days
Reference Range: See Report
Mercury, blood
Synonym: Hg
Test Mnemonic: MERC
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Plasma
Container Required: Navy (EDTA) tube
Volume of Specimen: 4 ml plasma
Minimum Volume of Specimen: 1 mL of plasma
Reasons for Rejection: SST tube
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Metanephrines, Plasma
General Use: Increased levels can be found in patients suffering from
pheochromocytoma, ganglioneuroma and neurogenic factors.
Test Mnemonic: METNP
Test Includes: Metanephrine, Normetanephrine, and total Metanephrine
Lab Performing Test: Specimen Processing, sent to Reference lab.
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Specimens must be drawn in “pre-chilled” tubes. Keep the
tubes on ice for transport to the lab! Send to lab ASAP!
Specimen Required: Plasma
Container Required: Two (2) lavender top pre-chilled EDTA tubes kept on ice.
Volume of Specimen: Entire collection tubes should be sent to lab
Minimum Volume of Specimen: 3-5ml plasma
Causes for Rejection: Specimen not drawn in pre-chilled tubes and/or not kept
on ice during transport.
Availability: Sent out Monday- Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Metanephrines, Total, 24 hour Urine
General Use: Evaluate catecholamine disorders
Test Mnemonic: UMET24
Test Includes: Metanephrine, Normetanephrine, total metanephrine
Lab Performing Test: Specimen Processing: sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: 24 hour urine
Special Instructions: Keep refrigerated during collection
Container Required: 24 hour urine container with preservative supplied by
Specimen Processing Lab
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: Medications patient is receiving are important in
interpretation
Methadone, urine
General Use: Methadone is used in the detoxification and maintenance programs
for physical dependence on narcotics. The analysis for methadone is useful in the
determination of compliance with maintenance program prescriptions, and in the
determination of dose effectiveness.
Test Mnemonic: METHU
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Urine
Container Required: Plastic sterile container
Volume of Specimen: 15 mL
Minimum Volume of Specimen: 5 mL urine
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Methotrexate Level
General Use: Monitor therapeutic drug level
Test Mnemonic: MTX
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Collect 24, 48, or 72 hours after dose
Specimen Required: Clotted Blood (Serum); protect from light; (wrap with
aluminum foil)
Container Required: Red top tube; protect from light at time of draw (Do not
use SST tubes) No gel barrier! Deliver immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Specimen not protected from light; gel barrier tube
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Methylenetetrahydrofolate Reductase
Synonyms: MTHFR
General Use: To assess a mutation in the methylenetetrahydrofolate gene which
regulates homocysteine; such a mutation could lead to a prothrombotic condition
Test Mnemonic: MTHFR
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. If specimen is drawn from an in-dwelling catheter, line must be
flushed with saline and the first 10 ml of blood collected must be discarded.
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: One full lavender top tube
Minimum Volume of Specimen: One full lavender top tube
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 14 days
Reference Range: See Report
Methylmalonic Acid
General Use: To confirm tissue cobalamin deficency in individuals with low
serum cobalamin levels
Test Mnemonic: METMAL
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Specimen must be sent to Main Lab within 1 hour of draw
time
Specimen Required: Clotted blood, serum
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely Required for Processing: 1 week
Reference Range: See report
MTHFR see Methylenetetrahydrofolate Reductase
Microsporidia Spore Detection
General Use: Detection of Microsporidia infection
Test Mnemonic: MSPOR
Test Includes: Smear and concentration
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool
Container Required: Sterile screw-cap container with 10% formalin ; Specimen
must be transferred to formalin vial within 30 minutes of collection
Volume of Specimen: 5g(5ml) of stool
Minimum Volume of Specimen: 5g (5ml) stool
Causes for Rejection: Unpreserved or frozen stool
Availability: Mon – Fri 7am to 3pm
Time Routinely Required for Processing: 5 days
Reference Range: None Detected
Multiple Sclerosis Panel
Synonyms: MSP, MS Panel
General Use: Evaluation of CSF for infections, degenerative or neoplastic
processes
Test Mnemonic: MSP1
Test Includes: Albumin (CSF and Serum), IgG (CSF and Serum), Oligoclonal
Bands CSF, and IgG Synthesis Rate/Index, CSF (calculated)
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Blood must be drawn at same time CSF is obtained
Specimen Required: Cerebrospinal fluid, clotted blood (serum)
Container Required: Clean glass or plastic container (CSF) and Red top (SST)
Volume of Specimen: 3 ml CSF and 10 ml blood
Minimum Volume of Specimen: 3 ml CSF, 2 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Mumps Antibodies
General Use: To aid in the diagnosis of infection with mumps virus
Test Mnemonic: MUMPS
Test Includes: Mumps Ab IgG and IgM
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Reasons for Rejection: Gross hemolyis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Myasthenia Gravis Panel
General Use: To aid in the diagnosis and management of Myasthenia gravis
Test Mnemonic: MYGR
Test Includes: Anti-Striated Muscle Antibody Screen with Reflex; Acetylcholine
Receptor Binding Antibody. If Anti-Striated Muscle Antibody Screen is positive,
Anti-Striated Muscle Antibody Titer will be performed at an additional charge.
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Mycoplasma Serology
Synonyms: Mycoplasma pneumoniae Titer
General Use: Used to support a diagnosis of Mycoplasma infection, atypical viral
pneumoniae
Test Mnemonic: MYCOAB
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Myelin Basic Protein
General Use: The pesence of myelin basic protein in the spinal fluid is supportive
evidence for the diagnosis of multiple sclerosis and other demyelinating diseases,
although it is a non-specific finding and present in other causes of damage to CNS
myelin.
Test Mnemonic: MBP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Cerebrospinal Fluid
Container Required: Sterile plastic container
Volume of Specimen: 1.2 ml CSF
Minimum Volume of Specimen: 0.5 mL
Causes for Rejection: Hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Myoglobin
General Use: The breakdown of skeletal muscle releases myoglobin
Test Mnemonic: MYO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10ml
Minimum Volume of Specimen: 0.5 ml serum
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Myoglobin, Urine
General Use: The breakdown of skeletal muscle releases myoglobin
Test Mnemonic: UMYOG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Urine- Deliver ASAP!
Container Required: Sterile Cup
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml urine
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Mysoline, Level
Synonyms: Primidone
General Use: Monitor therapeutic drug level
Test Mnemonic: PRIMA
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube - no gel barrier (SST) tubes
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Drawn in gel barrier tube
Availability: Sent Mon. - Sat. (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See report
Neisseria gonorrhoeae DNA, SDA
Synonym: Gonorrhea,GC
General Use: For detection of Gonorrhea
Test Mnemonic: GC
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Urine or endocervical, urethral or vaginal swab
Container Required: BD Transport or BD Urine Preservative Transport kit
Volume of Specimen: One swab or 15-60 ml of urine
Minimum Volume of Specimen: One swab or 15 ml of urine
Causes for Rejection: Swabs received in any other transport kit. Overfilled or
underfilled urine transport container. Cleansing swab received.
Availability: Sent out Monday-Saturday (not holidays)
Time Required Routinely for Processing: 4 days
Additional Information: Pink swab for vaginal specimens, blue swab for
urethral specimens
Reference Range: Not detected
Nickel
Test Mnemonic: NI
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Serum
Container Required: Royal blue top tube
Volume of Specimen: 2 mL
Minimum Volume of Specimen: 1 mL
Causes for Rejection: SST tube received, grossly hemolyzed
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 7 days
Reference Range: See Report
Niemann-Pick Disease Mutation Analysis
General Use: Niemann-Pick Disease, a debilitating and fatal disease, is caused by
an inherited deficiency of the enzyme acid sphingomyelinase. Niemann-Pick
Disease is an autosomal recessive disease with a carrier rate of 1 in 90 among
Ashkenazi Jews and a much lower carrier rate in other populations. Mutation
Analysis detects greater than 95% of mutations described in the Ashkenazi Jewish
population.
Test Mnemonic: NIEMAN
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep at Room Temperature!
Specimen Required: Whole blood
Container Required: Lavender top tubes (2)
Volume of Specimen: 2 Lavender top tubes
Minimum Volume of Specimen: 5 ml Whole blood
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Nortriptyline
Synonyms: Aventyl
General Use: Monitor therapeutic drug level
Test Mnemonic: NORT
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Collect just before next dose or at least 12 hours after last
dose.
Specimen Required: Clotted blood (serum)
Container Required: Red top with NO gel barrier!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Drawn in tube with gel barrier, gross hemolysis
Availability: Sent out Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-4 days
Reference Range: See report
Organic Acids, urine
General Use: Evaluation/Diagnosis of inborn error of metabolism
Test Mnemonic: ORGUR
Test Include: 78 organic acids. Contact the lab at x4153 for details
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Refrigerate during collection
Specimen Required: Random urine
Container Required: Urine cup, no preservative
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent out Mon-Thurs only
Reference Range: See Report
Ova and Parasites, Stool, Concentrate and Permanent Smear
Synonym: O and P with Permanent Stain
General Use: To aid in correct diagnosis of intestinal parasitic infection
Test Mnemonic: OAP
Lab Performing test: Specimen Processing; Sent to Reference lab
Request Form Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Interfering substances – bismuth,barium (wait 7-10 days)
Antimicrobial agents (wait 2 weeks), gallbladder dye (wait 3 weeks after
procedure)
Specimen Required: Stool
Container Required: 10% formalin transport vial and PVA transport medium
Volume of Specimen: Add stool to bring liquid level on vials to the “fill to
here” line
Minimum Volume of Specimen: Add stool to bring liquid level on vials to the
“fill to here” line
Causes for Rejection: unpreserved stool, specimens containing barium
and stools in vials other than 10% formalin and PVA
Availability: Mon-Fri 7a to 3p
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Oxalate, Urine
General Use: Renal function test; patients who form calcium oxalate kidney
stones appear to absorb and excrete a higher portion of their dietary oxalate in
urine than do normal subjects
Test Mnemonic: UOXA24
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should refrain from taking excessive amounts of
Ascorbic Acid or Oxalate-rich foods (i.e., spinach, coffee, tea, chocolate, rhubarb)
for at least 48 hours prior to the collection period.
Special Instructions: Specimen must be kept refrigerated during collection
Specimen Required: 24 hour urine
Container Required: Use 24 hour acidified urine container obtained from
Specimen Processing Lab
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See report
Paraneoplastic AB Evaluation
General Use: To aid in the diagnosis of paraneoplastic autoimmune
sensory/motor disorders
Test Mnemonic: PARANO
Test Includes: Hu Ab, Yo Ab
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent Mon - Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Parvovirus titers
Synonyms: Fifth's Disease titer, Parvovirus B-19
General Use: To rule out exposure to Parvovirus in pregnant females during first
trimester or aid in the diagnosis of parvovirus arthritis
Test Mnemonic: PARVO
Test Includes: Parvovirus IgG and IgM
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: Information must include estimated delivery date for
pregnant females
Reference Range: See report
Pentobarbital
Synonym: Nembutal
Test Mnemonic: PENTO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Collect just prior to next dose
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tube!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Reasons for Rejection: SST tube
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Phencyclidine
Test Mnemonic: PCP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tubes (2) – No gel tubes!
Volume of Specimen: 2 Red top (no gel) tubes
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Phenylalanine, PKU
Synonyms: Phenylketonuria test
General Use: PKU screen or monitor of diet
Test Mnemonic: PKU
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (plasma)
Container Required: Green top tube (Na Heparin); deliver ASAP!
Volume of Specimen: 3 ml whole blood
Minimum Volume of Specimen: 2 ml plasma
Availability: Sent Monday-Friday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Phenytoin, Free
Synonym: Free Dilantin
General Use: Free dilantin is a better estimate of the active drug level at the body
receptor sites.
Test Mnemonic: FDILA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube, No gel barrier. Deliver to Lab immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3 days
Reference Range: See Report
Phosphatidylserine Antibodies (IgG, IgM)
Test Mnemonic: PHOSAB
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Plasma, 3.2% sodium citrate
Container Required: Light blue top, 3.2% sodium citrate tube
Volume of Specimen: 5 mL
Minimum Volume of Specimen: 1 ml plasma
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Phospholipids
General Use: Phospholipids are used in diagnosing and treating disorders of lipid
metabolism associated with atherosclerosis.
Test Mnemonic: PHOSLP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Fasting required! Specimen must be centrifuged
within one hour of collection and poured off into plastic transfer tube
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gel barrier tube
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See Report
PNH with FLAER (High Sensitivity)
Synonym: Paroxsysmal Nocturnal Hemoglobinuria, GPI, PI Linked antigen
Test Mnemonic: PNHHS
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep at Room Temperature!
Specimen Required: Whole blood
Container Required: 10 mL green top, sodium heparin tube
Volume of Specimen: 10 mL
Minimum Volume of Specimen: 5 mL
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Poliovirus Antibody, Neutralization
General Use: This sensitive procedure is recommended for vaccine response
testing and type-specific serodiagnosis of recent poliovirus infection.
Test Mnemonic: POLAB
Testing Includes: Poliovirus Types 1, 2, and 3
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Serum
Container Required: Red top, SST tube
Volume of Specimen: 10 mL
Minimum Volume of Specimen: 2 mL
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 2-8 days
Reference Range: See Report
Porphobilinogen, 24 hour urine
General Use: Urinary Porphobilinogen is the first step in the diagnosis of acute
intermittent prophyria (AIP). AIP is an autosomal dominant disorder
characterized by deficiency of porphobilinogen deaminase. Acute attacks usually
include gastrointestinal disturbance and neuropsychiatric disorders.
Test Mnemonic: UPBG24
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep refrigerated during collection. Protect from light,
Deliver ASAP!
Specimen Required: 24 hour urine collection
Container Required: 24 hour urine container, no preservative
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Reasons for Rejection: Not protected from light
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 4 days
Reference Range: See Report
Porphyrins, Fractionated, Quantitative and Porphobilinogen, 24-Hour Urine
General Use: Evaluate porphyrias, lead poisoning
Test Mnemonic: UPOR24
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Do not expose to light; Keep refrigerated; Deliver
immediately after collection.
Specimen Required: 24 hr urine; refrigerate during collection time; protect from
light (wrap in aluminum foil)
Container Required: Special urine container supplied by Specimen Processing
lab
Volume of Specimen: Entire specimen
Minimum Volume of Specimen: Entire specimen
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Porphyrins, Fractionated, Quantitative, Random Urine
General Use: Evaluate porphyrias, lead poisoning
Test Mnemonic: UPORSC
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Do not expose to light; Refrigerated during collection.
Deliver immediately after collection.
Specimen Required: Random Urine (wrap in aluminum foil)
Container Required: Sterile urine cup protected from light
Volume of Specimen: Entire specimen
Reason for Rejection: Specimen not protected from light
Minimum Volume of Specimen: 2 mL Urine
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Primadone
Test Mnemonic: PRIMA
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Serum
Container Required: Red top no gel tube
Volume of Specimen: 2.0 mL
Reason for Rejection: SST (Gel) tube received
Minimum Volume of Specimen: 1 mL serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Prograf
Synonym: Tacrolimus
General Use: Immunosuppressant drug therapy
Test Mnemonic: PROGRA
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Optimal time to draw is 0.5-1 hour before next dose
Specimen Required: whole blood plasma
Container Required: Lavender top (EDTA)
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 2 ml whole blood
Reasons for Rejection: Clotted, SST tube
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Pro-Predict Metabolites
Synonym: Prometheus metabolites
General Use: Useful for monitoring patients on Immuran therapy
Test Mnemonic: PROPRE
Test Includes: 6-TG and 6-MMP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: 2 Lavender top (EDTA) tubes
Volume of Specimen: 5ml whole blood (2 tubes)
Minimum Volume of Specimen: 5 ml whole blood
Availability: Sent Monday-Friday (not holidays)
Time Required Routinely for Processing: 5-7 days
Reference Range: See Report
Protein Electrophoresis, Serum
Synonyms: SPEP
General Use: Screening test for serum protein abnormalities
Test Mnemonic: EPS
Test Includes: Total protein, protein electrophoresis
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Protein Immunoelectrophoresis, Urine
Synonyms: Urine Protein Electrophoresis;
General Use: This test is used to semi-quantitate Bence Jones proteinuria and to
study kidney disorders
Test Mnemonic: 24 hr collection: UIEP24; random urine: UIEP
Test Includes: Urinary protein fractionation
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: A serum protein electrophoresis should also be ordered for
a complete evaluation
Specimen Required: Random or 24 hr urine specimen
Container Required: Random: routine urine cup, for 24 hr collection obtain
container from Specimen Processing
Volume of Specimen: Entire collection
Availability: Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Prothrombin Gene Mutation
Synonyms: Factor II 20210A Mutation
General Use: Assess the presence of a gene mutation which has potential to
cause thrombosis
Test Mnemonic: PTGMUT
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. If specimen is drawn from an in-dwelling catheter, line must be
flushed with saline and the first 10ml of blood collected must be discarded.
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA) - 2
Volume of Specimen: 2 lavender top tubes
Minimum Volume of Specimen: 1 lavender top tube
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 8 days
Reference Range: See printed report
PTH, C Terminal
Synonyms: C Terminal Parathyroid hormone
General Use: Diagnosis of hypo and hyperparathyroidism
Test Mnemonic: PTHC
Test Includes: PTH, C Terminal and calcium
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting
Special Instructions: Draw specimen between 7:00AM. and 10:00AM.
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST). Deliver to Lab immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml serum
Causes for Rejection: Delay in receipt of specimen
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
PSA, Free and Total
General Use: To discriminate between prostate Ca and benign prostatic
hypertrophy (BPH)
Test Mnemonic: FTPSA
Test Includes: Total PSA, Free PSA, % Free PSA(calculated)
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST). Deliver to Lab immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Delay in transport of specimen, Gross hemolysis, lipemia,
icterus
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Pyruvic Acid
Synonym: Pyruvate
Test Mnemonic: PYR
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Call CP lab at ext 4153 before drawing blood!
Specimen Required: Whole blood, plasma. Requires special pre-treatment!
Container Required: Lavender (EDTA) - 2
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 4 ml whole blood
Causes for Rejection: Improper specimen treatment prior to transport
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See Report
Q Fever, Titer
Synonym: Coxiella burnetii
General Use: Used to support a diagnosis of Q fever due to Coxiella burneti
Test Mnemonic: QFEVER
Test Includes: Q fever Abs IgG and IgM with reflex to titers
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Quinidine, Blood
General Use: Monitor therapeutic drug level
Test Mnemonic: QUINI
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube -no gel barrier!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gel barrier tube (SST)
Availability: Mon-Sat. (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See report
Rapamycin
Synonym: Sirolimus
General Use: Monitor therapeutic drug level
Test Mnemonic: RAPA
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Optimal time to draw specimen is 0.5 to 1 hour before next
oral dose at steady state.
Specimen Required: Whole blood
Container Required: Lavender top EDTA tube
Volume of Specimen: 2 mL
Minimum Volume of Specimen: 1 mL whole blood
Causes for Rejection: Gel barrier tube (SST), clotted
Availability: Mon-Sat. (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See report
Renin Activity
Synonym: PRA
General Use: Useful in evaluating hypertension (renal and renovascular
hypertension)
Test Mnemonic: REN
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Follow doctor’s instructions since many factors are known
to influence plasma renin activity
Special Instructions: Deliver tube immediately- keep at room temperature!
Specimen Required: EDTA blood, plasma
Container Required: 2 Lavender top tube (EDTA)
Volume of Specimen: Two tubes (4ml each) whole blood (EDTA)
Minimum Volume of Specimen: 4 ml of plasma
Causes for Rejection: Improper tube/storage, delay in receipt of specimen;
lipemia, received refrigerated.
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report.
Respiratory Virus Panel by Direct Fluorescent Antibody Stain
General Use: Detection of Respiratory Viruses
Test Mnemonic: RESDFA
Test Includes: Influenza A,Influenza B, Para-Influenza 1/2/3,
Adenovirus, Respiratory Syncitial Virus, Metapneumo virus
Lab Performing Test: Specimen Precessing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional
Affiliate(HPA)
Specimen Required: Nasopharyngeal swab or wash, Bronch wash
Container Required: Viral Transport Media
Volume of Specimen:
Minimum Volume of Specimen:
Availability: 7 days per week (fall- winter flu season)
Time Required Routinely for Processing: Up to 2 days. Specimens
Received by 7pm resulted that day; after 7pm resulted the next day
Additional Information: RSV typing for A & B is not performed
Reference Range: See Report
Respiratory Virus Panel by PCR
General Use: For Detection of Respiratory Viruses. PCR detects unique
Genetic material from viruses
Test Mnemonic: RESPCR
Test Includes: Influenza A, Influenza B, Para-Influenza, Adenovirus,
Respiratory Syncitial Virus, Rhinovirus, Metapneumo virus
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional
Affiliate(HPA)
Specimen Required: Nasopharyngeal swab or wash, Bronch wash
Container Required: Viral Transport Media
Volume of Specimen:
Minimum Volume of Specimen:
Availability: 7 days (fall-winter flu season)
Time Required Routinely for Processing: Up to 2 days. Specimens
received by 7pm resulted that day; after 7pm resulted the next day
Additional Information: Can be sent to Yale or CT DPH lab. CT DPH
Lab tests for more viruses.
Reference Range: See Report
Reticulin Antibody, IgA
General Use: To aid in the diagnosis of Celiac disease
Test Mnemonic: RETIGA
Includes: If Reticulin antibody IgA screen is positive, Reticulin antibody IgA
titer will be performed at an additional charge.
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 0.5 ml serum
Reason for Rejection: Gross hemolysis, lipemia
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Ristocetin Co-Factor
General Use: Interpreted by physician
Test Mnemonic: RISTO
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting for at least 8 hours in preferred. Patient must not
be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Collection Instruction: Deliver to Lab immediately
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 1 blue top tube (tube must be full)
Minimum Volume of Specimen: 1 full blue top tube
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See printed report
Rubella Antibodies
General Use: To aid in the diagnosis of infection with Rubella (German measles)
virus
Test Mnemonic: RUBAB
Test Includes: Rubella Abs, IgG and IgM
Lab Performing Test: Specimen Processing; sent to reference labs
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Rubeola Antibodies
Synonyms: Measles titer
General Use: Used to support a diagnosis of Rubeola virus infection
Test Mnemonic: RUBEA
Test Includes: Rubeola IgG and Rubeola IgM
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Schistosoma Serology
General Use: To aid in the diagnosis of infection with Schistosoma parasite
Test Mnemonic: SCHIS
Test Includes: IgG and IgM antibodies
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Scleroderma Antibody
Synonyms: Antibody to ScL-70, Topoisomerase I
General Use: Useful in the diagnosis of Progressive Systemic Sclerosis (PSS)
Test Mnemonic: SCLER
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Reasons for Rejection: Gross hemolysis, lipemia, icterus
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Sex Hormone Binding Globulin
Synonyms: Testosterone binding globulin
General Use: Useful in the assessment of androgen Status
Test Mnemonic: SHBG
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Plasma, gross hemolysis
Availability: Sent Mon-Sat (Not holidays)
Reference Range: See Report
Serotonin, Blood
General Use: Evaluation of carcinoid syndromes
Test Mnemonic: SERO
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should avoid food high in indoles. Avoid avocados,
bananas, tomatoes, plums, walnuts, pineapple, and eggplant prior to testing.
Patient should also avoid tobacco, tea, and coffee three days prior to specimen
collection.
Specimen Required: Clotted blood (serum), deliver to Lab immediately!
Container Required: Red top tube, No gel barrier tubes!
Special Instructions: Deliver tube immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Sjogrens Antibodies
Synonyms: Antibodies to SS-A/Ro and SS-B/La
General Use: To aid in the diagnosis of SLE or Sjogrens Syndrome-Sicca
complex
Test Mnemonics: SJAB
Test Includes: SS-A, SS-B
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, lipemia, icterus
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Smooth Muscle Antibody
Synonym: Actin
General Use: Diagnostic test for lupoid hepatitis. Useful in the differential
diagnosis of primary biliary cirrhosis & chronic active hepatitis.
Test Mnemonic: SMA
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Reasons for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Somatostatin
General Use: Somatostatin is a neurohormone produced in the brain,
hypothalamus and gastrointestinal tract. This test is used to diagnose
somatostatin-producing tumors. Elevated levels are observed with
somatostatinoma, medullary thyroid carcinoma and pheochromocytoma.
Test Mnemonic: SOMAT
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Draw in a pre-chilled lavender top tube. Separate and
freeze immediately.
Specimen Required: Plasma (EDTA)
Container Required: Pre-chilled Lavender top EDTA tube
Volume of Specimen: 5 mL
Minimum Volume of Specimen: 2 mL plasma
Reasons for Rejection: Gross hemolysis, lipemia, icterus
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 3 weeks
Reference Range: See report
Stone Analysis
General Use: Evaluation of stone formation and composition.
Test Mnemonic: STONE
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stones
Container Required: Sterile screw top container
Volume of Specimen: Send stone specimen only. Do not send stone in urine
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: One week
Additional Information: Please indicate site from which stone is removed.
Reference Range: See report
Striated Muscle Antibodies
General Use: Diagnostic test for Myasthenia gravis
Test Mnemonic: STRMAB
Test Includes: If Striated Muscle Anitbody screen is positive, Striated Muscles
Antibody titer will be performed at an additional charge.
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: May also be ordered as part of Myasthenia Gravis Panel
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Rickettsia Antibodies
Synonyms: Rocky Mountain Spotted Fever Abs, Spotted Fever Typhus, RMSF
General Use: Support diagnosis of infection due to Rickettsia in the spotted fever
group
Test Mnemonic: ROCKY
Test Includes: If Rickettsia (RMSF) Antibodies (IgG, IgM) are positive, titers
will be performed at an additional charge
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Stool Osmolality, 24 hour
Synonym: Osmotic gap, feces
Test Mnemonic: STOSM
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
Specimen Required: 24, 48, 72 hour liquid stool
Container Required: Plastic leak-proof feces container
Volume of Specimen: Entire collection
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Stool Potassium, 24 hour
Synonym: Potassium, feces
Test Mnemonic: STOOLK
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep refrigerated during collection. Deliver ASAP!
Specimen Required: 24, 48, or 72 hour liquid stool
Container Required: Plastic leak-proof container
Volume of Specimen: Entire collection
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Stool Sodium, 24 hour
Synonym: Sodium, feces
Test Mnemonic: STNA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep refrigerated during collection. Deliver ASAP!
Specimen Required: 24, 48, or 72 hour liquid stool
Container Required: Plastic leak-proof container
Volume of Specimen: Entire collection
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Streptococcus pneumoniae IgG (23 Serotypes)
General Use: Responses to pneumococcal vaccines are demonstrated by 2- to 4fold increases in the levels of IgG recognizing approximately 70% of the
serotypes contained within a given pneumococcal vaccine.
Test Mnemonic: SPNU23
Test Includes: Serotypes 1, 2, 3, 4, 5, 8, 9 (9N), 12 (12F), 17 (17F), 14, 19 (19F),
20, 22 (22F), 23 (23F), 26 (6B), 34 (10A), 43 (11A), 51 (7F), 54 (15B), 56 (18C),
57 (19A), 68 (9V), 70 (33F)
Lab Performing Test: Specimen Processing; sent to reference labs
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Streptozyme
General Use: To demonstrate the presence of antibodies to a wide variety of
Streptococcal enzymes
Test Mnemonic: STRZ
Test Includes: If Streptozyme Screen is positive, Streptozyme titer will be
performed at an additional charge
Lab Performing Test: Specimen Processing; sent to reference labs
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Tay-Sachs Detection
Synonym: Hexosaminidase A & B
General Use: Test for "carrier state" of Tay-Sachs
Test Mnemonic: TAYHXA
Test Includes: Hexosaminidase A and B
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: Yellow ACD/2. Deliver ASAP!
Volume of Specimen: 20 ml
Minimum Volume Required: 20 ml Whole Blood
Availability: Sent Monday-Thursday ONLY
Time Required Routinely for Processing: 1 week
Reference Range: See report
Tay-Sachs Gene Mutation Analysis -Tay Sachs Mutation
General Use: Genetic Counseling
Test Mnemonic: TAYMUT
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Lavender top tube - draw 2. Keep room temperature
Special Instructions: Deliver tubes immediately to Lab
Volume of Specimen: 8 ml whole blood
Minimum Volume Required: 8 ml whole blood
Causes for rejection: Improper tube type or insufficient quantity
Availability: Sent Monday-Saturday (not holidays)
Reference Range: See report
Tetanus Antitoxoid Antibody
Synonym: Tetanus Antibody IgG
General Use: Used to evaluate Tetanus immunization efficacy.
Test Mnemonic: TETAB
Test Includes: Test for Tetanus Antitoxoid IgG
Lab Performing Test: Specimen Processing, sent to Reference lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: excessive hemolysis
Availability: Sent out Monday thru Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
THC Confirmation, Urine
Test Mnemonic: UTHCCO
Test Includes: Carboxy Acid THC, THC/Creatinine ratio, Creatinine
Lab Performing Test: Specimen Processing, sent to Reference lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Random Urine
Container Required: Sterile cup
Volume of Specimen: 20 mL
Minimum Volume of Specimen: 5 mL urine
Availability: Sent out Monday thru Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
ThinPrep Pap and HR HPV DNA
General Use: Liquid-based Pap Testing is intended for use in screening and
detection of cervical cancer, pre-cancerous lesions, atypical cells and all other
cytologic categories as defined by the Bethesda System for Reporting Results of
Cervical Cytology.
Test Mnemonic: PAP 15003
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Gynocological sample
Container Required: Pap sample collected in PreservCyt, alcohol-based
solution that serves as preservative, transport, and antibacterial medium for
samples.
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5-10 days negative, 7-12 days
abnormal
Reference Range: See report
ThinPrep Pap with reflex to HR HPV DNA
General Use: Liquid-based Pap Testing is intended for use in screening and
detection of cervical cancer, pre-cancerous lesions, atypical cells and all other
cytologic categories as defined by the Bethesda System for Reporting Results of
Cervical Cytology.
Test Mnemonic: PAP 31530
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Gynocological sample
Container Required: Pap sample collected in PreservCyt, alcohol-based
solution that serves as preservative, transport, and antibacterial medium for
samples.
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5-10 days negative, 7-12 days
abnormal
Reference Range: See report
ThinPrep Imaging Pap
Test Mnemonic: PAP 58315
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Gynocological sample
Container Required: Pap sample collected in PreservCyt, alcohol-based
solution that serves as preservative, transport, and antibacterial medium for
samples.
Volume of Specimen: Entire collection
Minimum Volume of Specimen: Entire collection
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5-10 days negative
Reference Range: See report
Thrombin Time
General Use: Determination of severe hypofibrinogenemia or qualitative
abnormality of fibrinogen
Test Mnemonic: TT
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Collection Instruction: Deliver to Lab immediately!
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 1 full blue top tube
Minimum Volume of Specimen: 1 ml plasma from full blue top tube
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See on printed report.
Thyroglobulin
Synonym: Thyroglobulin Antibodies
General Use: Thyroid disease testing
Test Mnemonic: THYRO
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Reasons for Rejection: Gross hemolysis
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See Report
Thyroglobulin Antibodies
Synonym: Antithyroglobulin Abs
General Use: Useful in the diagnosis and management of Thyroid disorders
Test Mnemonic: TGA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml sreum
Reasons for Rejection: Gross hemolysis
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Thyroid Auto-Antibodies
General Use: Diagnostic test for Hashimoto's thyroiditis; thyroid function test
Test Mnemonic: THYAA
Test Includes: Thyroglobulin Abs and Thyroid Peroxidase Abs
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Reason for Rejection: Gross hemolysis, lipemia
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Thyroid Peroxidase Ab
Synonym: Anti-thyroid Micrsomal Antibody, Anti-TPO
General Use: Thyroid disease testing
Test Mnemonic: TPO
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent Mon-Saturday (not holidays)
Reasons for Rejection: Gross Hemolysis
Time Required Routinely for Processing: 2-3 days
Reference Range: See Report
Thyroid Stimulating Immunoglobulin
Synonym: Long acting Thyroid stimulator, Thyroid Stimulating Immunoglobulin
General Use: Useful in the treatment of Graves Disease
Test Mnemonic: THYSIG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 0.2 ml
Reasons for Rejection: Gross hemolysis, lipemia, icterus, incorrect tube type
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Thyroxine Binding Globulin
Synonyms: TBG
General Use: Binding capacity for T-4
Test Mnemonic: TBG
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml of serum
Reason for Rejection: Gross Hemolysis
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Tissue Transglutaminase Antibody, IgG
Synonyms: tTg antibody (IgG)
General Use: Tissue Transglutaminase Antibody, IgG, is useful in diagnosing
gluten-sensitive enteropathies, such as Celiac Sprue Disease, and an associated
skin condition, dermatitis herpetiformis in patients who are IgA-deficient.
Test Mnemonic: TTGIGG
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis or gross lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See report
Topamax
Synonym: Topiramate
General Use: Topamax/Topiramate is an antidepressant used as an adjunctive
treatment of partial-onset epilepsy and Lennox-Gastaut syndrome in children.
Test Mnemonic: TOPA
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Draw at peak (2-4 hours after dose) or trough (0.5-1hour
before dose) at steady state. Do not use gel barrier tubes!
Special Instructions: Centrifuge tube within 1 hour of draw
Specimen Required: Clotted blood (serum)
Container Required: Red top tube, No gel barrier tubes! Deliver to Lab
immediately; tube must be centrifuged within 1 hour of draw.
Volume of Specimen: 10 mL
Minimum Volume of Specimen: 0.5 mL serum
Causes for Rejection: Improper tube type, delay in specimen processing
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Torch Panel
General Use: To support diagnosis of Torch infections in newborns or pregnant
females
Test Mnemonic: TORCH
Test Includes: Toxoplasmosis IgG and Igm, Rubella IgG and Igm,
Cytomegalovirus IgG and Igm, Herpes IgG and Igm.
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Two (2) red top tubes (SST)
Volume of Specimen: 15 ml
Minimum Volume of Specimen: 5 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Reference ranges provided with results
Toxicology Profile, Blood (Tox. Screen, Blood)
Synonyms: Toxicology Screen, Blood
General Use: Drug toxicity; toxicology screen
Test Mnemonic: DRGS
Test Includes: Call lab for complete listing of components
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: 2 red top tubes, No gel barrier!
Volume of Specimen: 15 ml blood
Minimum Volume of Specimen: 10 ml serum
Availability: Sent Monday-Saturday (not holidays)
Causes for Rejection: Gel barrier tubes
Time Required Routinely for Processing: 1 week
Reference Range: See report
Toxocara
Synonyms: VLM Titer; Toxocara Canis, Visceral larva migrans
General Use: Used to support a diagnosis of Visceral larva migrans
Test Mnemonic: TOXOC
Lab Performing Test: Specimen Processing; sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Negative
Toxoplasmosis Antibodies
Synonyms: Toxoplasma Serology
General Use: Used to support a diagnosis of Toxoplasmosis gondii
Test Mnemonic: TOXAB
Test Includes: Toxo IgG and Toxo Igm
Lab Performing Test: Specimen Processing; Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 mL
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Toxoplasmosis, Status
General Use: Used to determine immunity or past exposure to Toxoplasma
gondii
Test Mnemonic: TOXOIG
Test Includes: Toxoplasma IgG
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Reasons for Rejection: Gross hemolysis, lipemia
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Reported as immune or non-immune
TPMT
Synonym: Thiopurine Methyltransferase, Myelosuppresion, Myelotoxicity, SoftTPMT
General Use: Detection of individuals with low thiopurine methyltransferase
activity who are at risk for excessivr myelosuppression or severe hematopoietic
toxicity when taking azathioprine (Imuran) or 6-MP (Purinethol).
Test Mnemonic: TPMT
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Test should be ordered/drawn Mon – Thurs only
Container Required: Green top sodium heparin 10 ml tube
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 mL
Availability: Mon – Thurs only
Reference Range: See Report
TPMT (Thiopurine S-Methyltransferase) Genotype
General Use: This test detects common deficiency variants in the TPMT gene
and therefore identifies individuals who are at risk of developing serious adverse
effects when administered thiopurine drugs
Test Mnemonic: TPMTG
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Keep at room temperature. Do not spin.
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4 ml of blood (lavender top tube)
Minimum Volume of Specimen: 3 ml
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 9-10 days
Reference Range: See printed report
Transferrin
General Use: Differential diagnosis of anemia
Test Mnemonic: TRANS
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, lipemia
Availability: Sent Mon-Saturday (not holidays)
Time Required Routinely for Processing: 2-3 days
Reference Range: See Report
Trileptal Level
Synonym: Oxcarbazepine, 10-Hydroxycarbazepine
General Use: To monitor patient on Oxcarbazepine therapy
Test Mnemonic: TRIL
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube, No gel barrier tubes. Deliver to Lab
immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See Report
Tryptase
General Use: Tryptase concentrations are increased with immediate
hypersensitivity (anaphylaxis), acute allergen challenge, and mastocytosis.
Test Mnemonic: TRYPT
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver ASAP!
`
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – no gel!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, SST tube
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: Test performed on Tuesdays
Reference Range: See Report
TT see Thrombin Time
Urine Myoglobin
General Use: Interpretation by physician
Test Mnemonic: UMYO
Lab Performing Test: Specimen Processing, sent to Reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Send to L&M Lab immediately. Specimen must be frozen
for transport to Reference lab.
Specimen Required: Fresh urine
Container Required: Urine cup
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 10 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: Sent Mon-Sat, no holidays
Time Required Routinely for Processing: 3 days
Availability: Sent Mon-Sat, no holidays
Reference Range: See Report
Vanillylmandelic Acid, 24 Hour Urine
Synonyms: VMA
General Use: Diagnosis of catecholamine-producing tumors
Test Mnemonic: UVMA24
Test Includes: Creatinine
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: It is preferable for the patient to be off medications for
three days prior to collection. However, common antihypertensives (diuretics,
ACE inhibitors, calcium channel blockers, alpha and beta blockers) cause
minimal or no interference. Patient should avoid alcohol, coffee, tea, tobacco
(including use of nicotine patch), bananas, citrus fruits and strenuous exercise
prior to collection.
Specimen Required: 24 hour urine
Container Required: 24 hour urine container obtained from Specimen
Processing
Volume of Specimen: Entire specimen
Minimum Volume of Specimen: Entire specimen
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
VAP ™ Cholesterol panel
Synonym: Lipid Fractionation, Lipid Subparticle, Expanded Lipid Profile,
Expanded Lipid Subparticle, LDL Sub Fraction
General Use: The VAP ™ Cholesterol tests is a comprehensive test that includes
not only the standard lipid measurement but 21 lipid subfractions and emerging
risk factors such as Lp(a), IDL-C and LDL pattern size.
Test Mnemonic: VAP
Test Includes: Total Cholesterol, Direct LDL Cholesterol, HDL Cholesterol, and
direct measured Triglycerides, 21 Lipid Subfractions, Lp(a), IDL-C and LDL
pattern size, non-HDL cholesterol components: LDL, IDL, Lp(a), and VLDL
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Although 10-12 hour fast (no alcohol for 24 hours) is
required for the most accurate triglyceride measurement, all other values of the
VAP test are directly measured and fasting does not affect their outcome.
Special Instructions: Specimen must be centrifuged within 3 hrs of time of draw.
Deliver to Lab ASAP or call for processing instructions!
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.5 mL serum
Causes for Rejection: Improper specimen processing, Gross hemolysis
Availability: Sent Monday-Sat (not holidays)
Reference Range: See Report
Varicella Status
Synonyms: Varicella - immunity, Chicken pox titer, Herpes Zoster
General Use: To determine immunity or past infection with Varicella-Zoster
virus.
Test Mnemonic: VARIG
Lab Performing Test: Specimen Processing; sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: Reported as immune or non-immune
Varicella-Zoster, Antibodies
Synonyms: Chickenpox titer, Varicella zoster
General Use: Used to support a diagnosis of Herpes zoster-Varicella infection
Test Mnemonic: VARAB
Test Includes: Varicella Abs IgG and IgM
Lab Performing Test: Specimen Processing; Sent to Reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Vasoactive Intestinal Peptide
Synonym: VIP
General Use: VIP is a neurotransmitter and its excess can cause secretory
diarrhea. VIP-secreting tumors are most commonly found in the tail of the
pancreas in adults. VIP-producing ganglioneuromas or
ganglioneuroblastomas may be found in the adrenal glands in children.
Test Mnemonic: VASOP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver immediately!
Specimen Required: Plasma
Container Required: 2 - Lavender top (EDTA) tubes
Volume of Specimen: 2 - Lavender top (EDTA) tubes
Minimum Volume of Specimen: 3 ml plasma
Causes for Rejection: Lipemic samples not acceptable
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 9 days
Reference Range: See Report
VDRL-CSF
General Use: To aid in the diagnosis of latent syphilis
Test Mnemonic: VDSF
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Cerebrospinal fluid
Container Required: Clean sterile container
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml CSF
Causes for Rejection: Excessive contamination with RBC's
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: The VDRL slide test is still the only test recommended
by CDC for use with cerebrospinal fluid
Reference Range: Nonreactive
Virus Culture
General Use: For Detection of Viruses
Test Mnemonic: VIRC
Test Includes:
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate
(HPA)
Specimen Required: Skin swabs, biopsies, respiratory specimens, CSF
or stool
Container Required: Viral Transport Media
Volume of Specimen:
Minimum Volume of Specimen:
Availability:
Time Required Routinely for Processing:
Additional Information: MD must specify what viruses to test for.
Reference Range: See Report
Viscosity, Serum
Synonyms: Serum Viscosity
General Use: Detection of hyperviscosity syndromes associated with myeloma
and other dysproteinemias
Test Mnemonic: VISCO
Lab Performing Test: Specimen Processing; Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See report
Vitamin A, Blood
Synonyms: Retinol
General Use: Differential diagnosis of malabsorption syndromes; essential for
vision and bone growth
Test Mnemonic: VITAA
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred
Special Instructions: Protect specimen from light by wraping in foil
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST); protect from light!
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Not protected from light
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See report.
Vitamin B1
Synonym: Thiamine
General Use: Thiamine deficiency of toxicity
Test Mnemonic: VITAB1
Lab Performing Test: Specimen Processing, Sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver to lab immediately, protect tube from light!
Specimen Required: Whole blood, plasma
Container Required: lavender top (EDTA)
Volume of Specimen: 5 ml
Minimum Volume of Specimen: entire collection
Causes for Rejection: Exposure to light, delay in transport, improper tube type
Availability: Sent Mon-Saturday (no holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See Report
Vitamin B2
Synonym: Riboflavin
General Use: Rule out Riboflavin deficiency
Test Mnemonic: VITB2
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request form: Universal requisition; use only as a backup to Meditech
Specimen Required: Whole blood (plasma)
Container Required: Lavender top tube (EDTA); protect from light! Deliver
immediately!
Volume of Specimen: 4 ml whole blood
Minimum Volume of Specimen: 1 ml plasma
Causes for Rejection: Improper tube type, not light protected
Availability: Sent Mon.-Sat. (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: Specimen must be protected from light; Deliver immediately!
Reference Range: See report
Vitamin B6
Synonym: Pyridoxine, Pyridoxal, B6
General Use: Vitamin b6 deficiency may be observed in patients with metabolic
disorders secondary to drug use or alcoholism.
Test Mnemonic: VITB6
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting. Patient must be restricted from alcohol
and vitamins for at least 24 hours before sample collection.
Specimen Required: Whole blood (plasma)
Container Required: Lavender top tube (EDTA). Protect from light and deliver
to Lab immediately!!
Volume of Specimen: 5 ml whole blood
Minimum Volume of Specimen: 0.5 ml plasma
Causes for Rejection: Tube not protected from light. Delay in transport to Lab
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 6 days
Reference Range: See Report
Vitamin B12 Binding Capacity, Unsaturated
Synonym: Transcobalamin
General Use: Vitamin B 12 Binding Capacity, Unsaturated (Transcobalamin),
binds and transports vitamin B 12 in the circulation. Increased concentrations are
associated with patients with myeloproliferative disorders. Decreased
concentrations are associated with infants with megaloblastic anemia or
transcobalamin deficiency.
Test Mnemonic: VB12BI
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Cause for Rejection: Plasma
Availability: Sent out Mon-Sat (not holidays)
Time Required Routinely for Processing: 8 days
Reference Range: See Report
Vitamin C
Synonym: Ascorbic Acid
General Use: Evaluation of Vitamin C deficiency, scurvy
Test Mnemonic: VITAC
Lab Performing Test: Specimen Processing: sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting is preferred. Patient should refrain from
taking vitamin C supplements or fruits 24 hours prior to sample collection.
Special Instructions: Deliver to lab immediately, protect tube from light!
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST); protect from light! Deliver
immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Not light protected
Availability: Sent out Mon.-Sat. (not holidays)
Time Required Routinely for Processing: 1 week
Additional Information: Specimen must be protected from light; Deliver
immediately!
Reference Range: See report
Vitamin D1, 25-Dihydroxy
General Use: Deficiencies of 1,25-dihydoxy Vitamin D, the most active form,
causes hypocalcemia, osteomalcia and related disorders.
Test Mnemonic: VD125
Test Includes: Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin
D2, 1,25 (OH)2
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST) Keep tube refrigerated after drawing!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.5 ml serum
Causes for Rejection: Improper storage temperature.
Availability: Sent Monday-Sat (not holidays)
Time Required Routinely for Processing: 5 days
Reference Range: See Report
Vitamin D, 25 Hydroxy fractionation
General Use: Measurement of serum 25-OH vitamin D concentrations provide a
good index of circulating vitamin D activity in patients not suffering from renal
disease. Lower than normal 25-OH vitamin D levels can result from a dietary
deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in
the liver.
Test Mnemonic: VD25FR
Test Includes: Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D 3; Vitamin D, 25OH, D2
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting preferred, but not required
Specimen Required: Clotted blood (serum)
Container Required: Red top tube – No gel tube!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Sent out Mon-Sat (not holidays)
Reference Range: See Report
Vitamin E
Synonym: Alpha-Tocopherol
General Use: Useful in evalutation of Vitamin E deficiency in pre-maturity,
cystic fibrosis and mal-absorption syndromes
Test Mnemonic: VITAE
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Over night fasting is preferred
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST); protect from light and deliver
immediately!
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Not light protected, delay in receipt of specimen
Availability: Sent out Mon.-Sat. (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Vitamin K
General Use: Vitamin K is a required co-factor for the synthesis of factors 2, 7, 9,
and 10 and proteins C and S. Deficiencies of vitamin K lead to bleeding.
Coumadin® (warfarin) acts as an anticoagulant because it is a vitamin K
antagonist.
Test Mnemonic: VITK
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Over night fasting is preferred
Specimen Required: Plasma (EDTA)
Container Required: Lavender top (EDTA); protect from light and deliver
immediately!
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 2 ml plasma
Causes for Rejection: Not light protected, delay in receipt of specimen
Availability: Sent out Mon.-Sat. (not holidays)
Time Required Routinely for Processing: 1 week
Reference Range: See report
Zinc, Blood
Synonyms: Zn
General Use: Zinc toxicity
Test Mnemonic: ZI
Lab Performing Test: Specimen Processing; sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Navy Blue top with EDTA as preservative; must be spun
within 2 hours of collection; Deliver ASAP!
Volume of Specimen: 5.0 ml whole blood
Minimum Volume of Specimen: 1.0 ml plasma
Causes for Rejection: Improper tube type; hemolysis
Availability: Sent Monday-Saturday (not holidays)
Time Required Routinely for Processing: 3-5 days
Reference Range: See report
REFERENCES: Quest Diagnostics Test Menu
CHEMISTRY
The section is directed by Victoria G. Reyes-D'Arcy, M.D. and supervised by Barbara
Naillis. Most tests are available on a daily basis Monday through Sunday from 7am to 11
pm. STAT testing, where applicable, is available on a 24 hour basis.
For Chemistry services call ext 5144, for Serology services call ext 5119. The
Chemistry/Serology supervisor can be reached at ext 3780.
Acetaminophen, Blood
Synonyms: Tylenol
General Use: Acetaminophen toxicity, acetaminophen poisoning
Test Mnemonic: TYL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: Therapeutic range 10-30 ug/ml.; >150 ug/mL is potentially
toxic
Acetone, Quantitative, Blood
Synonyms: Blood ketones
General Use: Evaluation of ketonemia
Test Mnemonic: ACET
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Do not use alcohol to clean venipuncture site.
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml Serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: Negative
Albumin, Blood
Synonyms: ALB
General Use: Evaluate serum protein, nutritional status, renal and liver function
Test Mnemonic: ALB
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: 3.4-5.0 gms/dl
Albumin, Fluid
Test Mnemonic: FALBU
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate source when ordering
Container Required: Sterile glass/plastic tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5ml
Causes for Rejection: Viscous or clotted sample
Availability: Daily 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Reference ranges not established
Alcohol, Blood
Synonyms: Blood Ethanol, ETOH
General Use: Evaluation of alcohol intoxication
Test Mnemonic: ALC
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Do not use alcohol to clean venipuncture site
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Additional information: "Chain of Custody" protocol not followed. Test offered
for medical (non-legal) evaluation only.
Reference Range: None detected (Legal intoxication > .08 gm/dL)
Alcohol, Urine
Synonyms: Urine ethanol
General Use: Alcohol ingestion
Test Mnemonic: UALC
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Urine container, not just lid, must have patient
identification
Specimen Required: Freshly voided urine
Container Required: Clean urine specimen container
Volume of Specimen: 30 ml
Minimum Volume of Specimen: 10 ml, 30 ml required if confirmation is
requested
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Additional information: "Chain of Custody" protocol not followed; test offered
for medical (non-legal) evaluation only.
Reference Range: None detected
Alkaline Phosphatase, Blood
Synonyms: ALP
General Use: Liver function; Metabolic bone disorders
Test Mnemonic: ALKP
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: 50-136 U/L for Adults Levels in Children are 2-3 times Adult
range
Alpha feto protein, tumor marker
General Use: Useful in the dx and management of germ cell and hepatic
carcinomas
Test Mnemonic: AFPTM
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis or chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs, Stat 1 hour
Reference Range: See report
Ammonia, Blood
Synonyms: NH3
General Use: Liver function test
Test Mnemonic: NH3
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Vacutainer tube should be placed on ice and brought to
laboratory immediately after drawing
Specimen Required: Heparinized Blood (Plasma)
Container Required: Li Heparin green top vacutainer tube (heparin). Place on
ice; deliver to Lab immediately.
Volume of Specimen: 4 ml
Minimum Volume of Specimen: 1.0 ml plasma
Causes for Rejection: Specimen received past 30 minute after collection;
specimen not received on ice
Availability: daily 24 hrs
Time Required Routinely for Processing: 1 hour
Reference Range: <32 umol/L
Amniotic Fluid L/S Ratio
Synonyms: L/S Ratio
General Use: Test for fetal maturity
Test Mnemonic: LS
Test Includes: Lecithin/Sphingomyelin Ratio, fluid creatinine, PG by TLC
Lab Performing Test: Chemistry/Serology, sent to Yale
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Amniotic fluid should be placed in a dark container to
reduce light effect. Call Chemistry laboratory to schedule test arrangements. Ext
5119
Specimen Required: Amniotic fluid – specimen must be received in lab no later
than 10am for same day results!
Container Required: Dark brown plastic tube
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 mL
Availability: Mon-Sun
Time Required Routinely for Processing: 8 hours
Additional information: Blood in the specimen may alter the ratio. Bloody taps
are questionable. Vaginal pools are not acceptable for L/S ratio testing
Reference Range: See Report
Amylase, Blood
Synonyms: Amy
General Use: Pancreatic disease
Test Mnemonic: AMYL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top vacutainer (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: 25-115 U/L
Amylase, Body Fluids
General Use: Pancreatic disease
Test Mnemonic: FAMYL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Specify source when ordering
Specimen Required: Fluid (no preservatives)
Container Required: Any clean dry container
Causes for Rejection: Viscous or clotted sample
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours. Stat 1 hour
Reference Range: Not established
Amylase, Urine random
General Use: Pancreatic function
Test Mnemonic: UAML
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: For 24 hour collections, call the Specimen Processing Lab.
Specimen Required: Random urine specimens
Container Required: Any clean container (no preservative)
Volume of Specimen: 5 mL
Minimum Volume of Specimen: 1 mL
Availability: 24 hrs daily
Time Required Routinely for Processing: 8 hours
Reference Range: Not established
Anti-Centromere Antibody
Synonyms: Anti-Kinetochore Ab
General Use: To aid in the diagnosis of patients with CREST syndrome. Also
exhibited by one third of patients with Raynaud's disease
Test Mnemonic: CENT
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Gross hemolysis
Availability: Mon – Fri 7am – 3pm. Not STAT
Time Required Routinely for Processing: 2 days
Reference Range: Negative
Anti-Gliadin Antibodies, IgA and IgG
Test Mnemonic: GLIAB
Test Includes: Anti-gliadin antibody IgA and Anti-gliadin antibody IgG
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Mon – Fri 7:00am-3:00pm
Time Required Routinely for Processing: 72 hours
Reference Range: See Report
Arterial Blood Gas
Synonyms: Blood Gas, Complete
Test Mnemonic: ABG
Test Includes: pH, pCO2, HCO3
Lab Performing Test: Respiratory Therapy and Point of Care (Chemistry)
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: On required O2
Special Instructions: Notify lab if on anticoagulants
Specimen Required: Arterial Blood
Container Required: Heparinized syringe
Volume of Specimen: 3 ml
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hours per day; Stat or routine
Time Required Routinely for Processing: 30 minutes
Reference Range: See report
Arterial pH
Synonyms: Blood pH
Test Mnemonic: BPH
Lab Performing Test: Respiratory Therapy and Point of Care (Chemistry)
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Notify lab if on anticoagulants;
Specimen Required: Arterial or venous blood
Container Required: Heparinized syringe or green top tube (Heparin)
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 85uL
Availability: Stat: 24 hours per day or routine
Time Required Routinely for Processing: 30 minutes
Reference Range: Arterial 7.35-7.45
Basic Metabolic Panel
General Use: Metabolic Screening
Test Mnemonic: BASIC
Test Includes: Glucose, BUN, Creatinine with GFR, Calcium, Electrolytes , gap
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Specimen grossly hemolyzed or lipemic
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See individual test reports
Bilirubin, Direct
Synonyms: Conjugated bilirubin
General Use: Liver function
Test Mnemonic: DBIL
Lab Performing Test: Chemistry
Request Form: : Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: For Neonates Protect from light!
Specimen Required: clotted blood (serum)
Container Required: 1 pediatric microtainer tube or Red top tube (SST)
Minimum Volume of Specimen: 0.1 ml serum for neonates or 1 ml from SST
Causes for Rejection: Extreme hemolysis , hemolysis will falsely depress values.
Availability: 24 hours daily
Time Required Routinely for Processing: Routine: 2 hours; Stat: 1 hour
Reference Range: See report
Bilirubin, Total (Neonatal)
Synonyms: NBIL
General Use: Liver function
Test Mnemonic: TBIL
Test Includes: On newborns (less than 21 days old) If total is >15 mg/dl, a direct
bilirubin is performed
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Protect from light
Specimen Required: Clotted blood (serum)
Container Required: 1 pediatric (microtainer) tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.1 ml serum
Causes for Rejection: Extreme hemolysis of specimen
Availability: 24 hours daily
Time Required Routinely for Processing: Routine, 2 hours; Stat, 1 hour
Reference Range: See report
Bilirubin, Total and Direct, Blood
Synonyms: Total and Conjugated Bilirubin;
General Use: Liver function
Test Mnemonic: BILI
Test Includes: Total and direct Bilirubin
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: For Neonates Protect from light!
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1.0 ml serum
Causes for Rejection: Extreme hemolysis of specimen
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Direct<0.2 mg/dL: Total <1.0 mg/dl
Blood Oxygen Saturation
Synonyms: Arterial O2 Saturation
Test Mnemonic: O2SAT
Lab Performing Test: Respiratory Therapy and Point of Care (Chemistry)
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: On required O2 for minimum of 20 minutes
Special Instructions: Notify lab if on anticoagulants; specimen must be
transported on ice
Specimen Required: Arterial blood
Container Required: Heparinized syringe
Volume of Specimen: 3 ml
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hours daily
Time Required Routinely for Processing: 30 minutes
Reference Range: See report
BNP
Synonym: Brain natriuretic peptide
General Use: Aid in the diagnosis of CHF
Test Mnemonic: BNP
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top SST tube
Volume of Specimen: 10 ml of clotted blood
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: gross hemolysis
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, STAT 1 hour
Reference Range: Age dependent; See report
C3 Complement
Synonyms: C3
General Use: Serum complement evaluation is useful in the diagnosis of several
disease states
Test Mnemonic: CC3
Lab Performing Test: Chemistry/Serology
Request Form: : Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Causes for Rejection: Gross hemolysis, chylous serum
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: Age/gender dependent; See report
C4 Complement
Synonyms: C4
General Use: Serum complement evaluation is useful in the diagnosis of several
disease states
Test Mnemonic: CC4
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis; chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: Age /gender dependent; See report
CA-125
Synonyms: Carbohydrate antigen-125
General Use: Useful for monitoring therapy and confirming biopsy of certain
carcinomas
Test Mnemonic: CA125
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon - Fri 7am-5pm; Sat 7am-3pm
Time Required Routinely for Processing: 24 hrs
Reference Range: See report
Calcium, Blood
Synonyms: Ca
General Use: Endocrine disorders; metabolic disorders
Test Mnemonic: CA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: 8.5-10.1 mg/dl
Calcium, Serum, Ionized
Synonyms: Ionized Calcium
Test Mnemonic: IONCA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Special Instructions: Allow specimen to clot at room temperature for 30
minutes. Then spin tube,keep refrigerated and deliver to lab ASAP.
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml
Availability: 24 hours daily
Additional information: Tube may not be shared with other lab tests
Time Required Routinely for Processing: 1 hour
Limitations: Total calcium remains the first line test for evaluation of calcium
abnormality
Reference Range: See report
Calcium, Urine
Synonyms: Urinary Calcium
General Use: Evaluation of calcium metabolism
Test Mnemonic: 24 hour collection-UCA24: random specimen-UCA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: 24 hour urine collection or random specimen
Container Required: Yellow top urine tube or Urine cup for random. 24 hr.
urine container supplied by Chemistry lab
Volume of Specimen: Entire specimen
Availability: Daily 7am-11pm for 24 hour collection; 24 hrs daily for random
collection
Time Required Routinely for Processing: 1 Day for 24 hour collection;
Random collection: Routine 8 hrs; Stat 1 hour
Reference Range:
See Report
Carbon Dioxide, Total
Synonyms: CO2
General Use: Evaluation of acid - base balance
Test Mnemonic: CO2
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hrs, Stat 1 hour
Reference Range: 21-32 mmol/L
Carboxyhemoglobin
Synonyms: Blood CO Level
Test Mnemonic: COHGB
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Specimen must be transported on ice
Specimen Required: Whole blood (heparinized)
Container Required: Heparinized syringe, green top tube
Volume of Specimen: 3 ml
Minimum Volume of Specimen: 85 ul
Availability: 24 hrs. daily
Time Required Routinely for Processing: 30 minutes
Reference Range:
Suburban Nonsmoker: <1.5% of tHGB
Smokers: 1.5-5.0% of tHGB
Heavy Smokers: >5.0-9.0% of tHGB
Carcinoembryonic Antigen
Synonyms: CEA
General Use: For follow up of patients with neoplasm, to evaluate and monitor
anti-neoplastic therapy
Test Mnemonic: CEA
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: See report
CCU Profile
General Use: Cardiac evaluation for MI
Test Mnemonic: CCU
Test Includes: CBC, Glucose, Bun, Creatinine with GFR, Electrolytes, gap,
Sgot/Ast, Magnesium, Heart (Total CPK and Troponin I)
Lab Performing Test: Hematology and Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole Blood (Plasma) and Clotted Blood (Serum)
Container Required: Lavender top (EDTA), Red top (SST) and Green top (Li
Heparin)
Volume of Specimen: 5 ml Whole Blood, 10 ml Clotted Blood
Minimum Volume of Specimen: 5 ml Whole Blood and 2 ml Serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: See individual test reports
Celiac Profile
Test Mnemonic: CELIAC
Test Includes: Anti-gliadin antibodies, IgA and IgG; total IgA; IgA tTg
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Mon - Fri 7:00AM-3:00PM
Time Required Routinely for Processing: 72 hours
Reference Range: See Report
Childhood Allergy Panel
Synonym: Allergy testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: CALPAN
Test Includes: For a complete listing of the allergens included in the panel, call
Serology at 444-5119. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon-Fri 7am to 3pm
Time Required Routinely for Processing: 48 hrs
Reference Range: Interpretation provided with results.
Chloride, Blood
General Use: Evaluate electrolyte balance
Test Mnemonic: CL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: 98-107 mmol/L
Chloride, CSF
General Use: CSF chloride may be lowered in tuberculosis
Test Mnemonic: CSFCL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Submit serum sample for chloride determination for proper
Interpretation of CSF chloride
Specimen Required: Cerebrospinal fluid
Container Required: Spinal fluid tube
Volume of Specimen: 1.0 ml
Minimum Volume of Specimen: 0.5 ml
Availability: Stat; 24 hours daily
Additional information: Deliver to laboratory immediately!
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: Reference ranges not established.
Chloride, Urine
Synonyms: CL Urine
General Use: Electrolyte balance studies
Test Mnemonic: 24 hour collection-UCL24; random specimen-UCL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Timed or random urine collected by nursing staff
Specimen Required: Timed or random urine
Container Required: 24 hour urine container supplied by laboratory; Yellow top
urine tube or urine cup for random
Volume of Specimen: Entire specimen
Minimum Volume of Specimen: Entire specimen
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See Report
Cholesterol, Total
Synonyms: Serum Cholesterol
General Use: Lipid screening
Test Mnemonic: CHOL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: 12-14 hours fasting: should be on stable diet for 3 weeks
prior to collection
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Non fasting sample
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range:
Desirable: <200 mg/dL
Borderline High: 200-240 mg/dL
High: >or= 240 mg/dL
Cholesterol, Fluid
Test Mnemonic: FCHOLE
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate source when ordering
Container Required: Sterile glass/plastic tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml
Causes for Rejection: Viscous or clotted sample
Availability: 24 hrs. daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: Reference ranges not established
Cholesterol Fractionation
Synonyms: CHOF
General Use: Cholesterol screening
Test Mnemonic: LIPFRA
Test Includes: Total Cholesterol, Triglylcerides, HDL and calculated LDL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: 12 - 14 hours fasting: Should be on stable diet for 3 weeks
prior to test
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Non fasting sample
Availability: 24 hrs. daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See individual reports
CKMB (Creatine Kinase, MB Fraction)
Synonyms: MMB, CPK2
General Use: To document myocardial infarction
Test Mnemonic: CKMB
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml
Availability: 24 hrs Daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hr
Reference Range: <3.5 ng/mL
Comprehensive Inhalant Panel
Synonyms: Allergy testing
General Use: To aid in diagnosis of atopic allergies
Test Mnemonic: CIP
Test Includes: Screening for most common trees, grasses, weeds, mold and
animal allergens. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 4 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon-Fri 7AM to 3PM
Time Required Routinely for Processing: 48 hours
Additional information: A complete listing of the allergens included is available
from the lab. Call 444-5119
Reference Range: Interpretation provided with results
Comprehensive Metabolic Panel
Synonyms: Chem panel
General Use: Metabolic Screening
Test Mnemonic: COMP
Test Includes: Glucose, BUN, Creatinine with GFR, Calcium, Total Protein,
Albumin,Globulin, Total Bilirubin Alkaline Phosphatase, SGOT, SGPT,
Electrolytes ,gap
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting for optimum results
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Specimen grossly hemolyzed or grossly lipemic
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: See individual test reports
Cortisol
General Use: Diagnosis of adrenocortical insufficiency (Addison's disease) and
adrenocortical hypersecretion (Cushing's disease)
Test Mnemonic: CORT
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon - Fri 7AM-5PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hrs
Reference Range: Before 10am 4.5-22.7 ug/dL
After 5pm 1.7-14.1 ug/dL
CPK
Synonyms: Creatine Kinase
General Use: Cardiac damage, muscle disease, brain damage, acute pulmonary
diseases
Test Mnemonic: CK
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: Females: 26-192 U/L
Males: 39-308 U/L
C-Reactive Protein
Synonym: CRP
General Use: CRP has been detected in the serum or patients with inflammation
or necrosis.
Test Mnemonic: CRP
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: None
Special Instructions: None
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume Required: 1 ml serum
Causes for Rejection: Gross hemolysis or chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Additional information: None
Reference Range: <0.30 mg/dL
C-Reactive Protein, Cardiac
Synonym: High sensitivity CRP
General Use: Evalution for risk of heart disease
Test Mnemonic: HSCRP
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: None
Special Instructions: None
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume Required: 1 ml serum
Causes for Rejection: Gross hemolysis or chylous serum
Availability: 24 hrs daily.
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: See patient report
Creatinine, Blood
General Use: Kidney function
Test Mnemonic: CRETP
Test Includes: GFR calculation
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 0.6 – 1.3 mg/dL
Creatinine Clearance
Synonyms: 24 hr creatinine clearance
General Use: Renal function
Test Mnemonic: CCLR
Test Includes: Serum creatinine, Urine creatinine
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: For best results, no meat, tea, coffee or drugs should be
consumed on day of test
Special Instructions: Blood creatinine must be drawn within 72 hr of urine
collection. Urine container must have collection times on it. ( keep refrigerated
during collection)
Specimen Required: 24 hr urine collection and 10 ml clotted blood (serum)
Container Required: 24 hour urine container obtained from Chemistry and red
top tube (SST)
Volume of Specimen: Entire timed urine collection and 3 ml serum
Minimum Volume of Specimen: Entire collection
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour.
Reference Range: See Report
Creatinine, 24 hour Timed or Random Urine
Test Mnemonic: 24 hr collection - UCR24; random specimen UCREAT
General Use: Kidney function
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Observe usual 24 hr urine collection instructions (keep
refrigerated during collection)
Specimen Required: Random or timed 24 hour urine collection
Container Required: 24 urine container obtained from Chemistry. Urine cup or
yellow top urine tube for random collection
Volume of Specimen: Entire collection for 24 hr and 10 ml for random
Minimum Volume of Specimen: Entire specimen for 24 hr and 1 ml for random
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See Report
Digoxin, Blood
Synonyms: Lanoxin
General Use: Diagnosis of digoxin toxicity
Test Mnemonic: DIG
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Specimen should be collected at least 6 hrs after the last
oral dose.
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs. daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Therapeutic range : 0.8-2.0 ng/mL
Dilantin, Blood
Synonyms: Phenytoin
General Use: Monitor therapeutic drug levels
Test Mnemonic: DILA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Therapeutic: 10-20 ug/mL; > 30 ug/mL is potentially toxic
Drugs of Abuse, Urine
Synonyms: UDS
General Use: Drugs of abuse toxicity; drug poisoning
Test Mnemonic: DA
Test Includes: Urine: Amphetamines, Barbiturates, Benzodiazepines, THC,
Cocaine, Opiates and Methadone
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Fresh voided urine
Container Required: Clean urine specimen container or yellow top urine tube,
urine container and lid must have patient identification
Volume of Specimen: 30 mls
Minimum Volume of Specimen: 10 mls; 30 mls urine required if confirmation is
requested
Availability: Daily 24 hrs
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Additional information: 'Chain of Custody' protocol not followed; screen
offered for medical (non-legal) purposes only: Confirmation of positive results
available by request, call ext 5144
Reference Range: Negative
Electrolytes, Blood
General Use: Electrolyte balance
Test Mnemonic: LYTES
Test Includes: Na, K, CI, CO2 and anion gap
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Extreme hemolysis
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See individual test reports
Electrolytes, Urine
Synonyms: Lytes, Urine
General Use: Renal function
Test Includes: Random Urine Sodium, Potassium and Chloride
Test Mnemonic: ULYTES
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Random urine collection
Specimen Required: Random urine
Container Required: Yellow top urine tube or random urine cup
Volume of Specimen: 10 ml urine
Minimum Volume of Specimen: 1 ml for random
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See Report
Estradiol
Synonyms: E2
General Use: Assessment of hypothalamus, pituitary, and ovarian function
Test Mnemonic: ESTRA
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Mon - Fri 7AM-5PM. Sat 7am-3pm. May be ordered STAT
Time Required Routinely for Processing: 24 hrs
Reference Range: See report
Ferritin, serum
General Use: Screening for iron deficiency anemia
Test Mnemonic: FERR
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: None
Specimen Required: Clotted Blood (Serum).
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon-Fri 7AM-5PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hrs
Reference Range: See Report
Folate, serum
Synonyms: Folic Acid (Serum)
General Use: Diagnosis of folate deficiency; monitoring folate therapy
Test Mnemonic: FOL
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should be fasting overnight
Specimen Required: Clotted Blood (Serum).
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Grossly hemolyzed specimen
Availability: Mon-Fri 7AM-5PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hrs
Reference Range: >2.7 ng/mL
Follicle Stimulating Hormone
Synonyms: FSH
General Use: Assessment of pituitary function and to distinguish primary from
secondary gonadal failure
Test Mnemonic: FSH
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon-Fri 7AM-5PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hours
Reference Range: FSH ranges vary with age and phase of menstrual cycle.
Ranges are provided with results.
Free and Total Prostate Specific Antigen
Synonyms: Free and Total PSA
General Use: To aid in the diagnosis and monitoring of Prostatic Ca and BPH
Test Mnemonic: FRTPSA
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container required: Red top tube (SST)
Volume of Specimen: 10ml
Minimum Volume of Specimen: 1ml
Causes for Rejection: Gross hemolysis or chylous serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; STAT 1 hour
Reference Range: See Report
General Food Screen
Synonyms: Allergy testing
General Use: To aid in diagnosis of atopic allergies
Test Mnemonic: GFS
Test Includes: Allergy screening for milk, eggs, wheat, peanut, soybean and
corn. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon-Fri 7AM to 3PM
Time Required Routinely for Processing: 48 hours
Reference Range: Interpretation provided with test results
GGT
Synonyms: SGGT, GGTP
General Use: Liver function test which parallels liver alkaline phosphatase
Test Mnemonic: GGT
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Males: 15-85 U/L; Females: 5-55 U/L
Gentamicin
Synonyms: Garamycin
General Use: Monitor therapeutic level
Test Mnemonic: Trough: GENT; peak: GENP; random: GENR
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Peak level: draw specimen 30 minutes after end of 30
minute I.V. infusion; Trough level: draw immediately prior to next dose
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 mL serum
Availability: Daily 24 hrs
Time Required Routinely for Processing: 2 hours
Additional information: Specimens should be drawn at steady state, usually
after fifth dose, if drug given every 8 hours, or after third dose, if given every 12
hours; Gentamicin has significant nephrotoxicity and ototoxicity
Limitations: Toxic levels may be reflective of, rather than predictive of, renal
damage.
Reference Range: Therapeutic Peak: 4-8 ug/ml, Trough: 0.0-2.0 ug/ml.
Glucose, Blood
Synonyms: Fasting blood sugar, Non-fasting blood sugar
General Use: Diabetes mellitus; Carbohydrate metabolism disorders
Test Mnemonic: Fasting: FBS; Non-fasting (Casual): GLUCR
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: FBS - Patient should fast overnight 8-12 hours; NPO after
11pm.
Special Instructions: Non-fasting glucose – If order is for "post-prandial"
testing instruct patient to eat a meal 1 hr. prior to collection.
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See Report
Glucose, Fluid
Test Mnemonic: FGLU
Test Includes: Fluid glucose
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Specify source when ordering
Specimen Required: Body fluid, specify source
Container Required: Sterile glass/plastic tube
Volume of Specimen: 2 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Viscous or clotted specimen
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Reference ranges not established
Glucose, OB screen
General Use: For OB patients who do not show diagnostic elevations of fasting
or casual glucose
Test Mnemonic: GOBS
Test Includes: Single blood sample for glucose drawn one hour after 50gm oral
dose of glucose
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: This test does not require fasting
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1 ml. serum
Cause for Rejection: Grossly hemolyzed blood
Availability: Daily
Time Required Routinely for Processing: Routine 8 hrs.
Reference Range: See report
Glucose, Spinal Fluid
Synonyms: CSF Glucose
General Use: Diagnosis of central nervous system disorders
Test Mnemonic: CSFG
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Physicians responsibility
Special Instructions: Specimen brought to laboratory immediately by nursing
staff.
Specimen Required: Spinal fluid
Container Required: Clean test tube
Volume of Specimen: 1 ml.
Minimum Volume of Specimen: 0.3 ml
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Additional information: Suggest simultaneous blood glucose
Reference Range: 40-70 mg/dL
Glucose Tolerance OB Patients
Synonyms: GTOB
General Use: Diagnosis of Gestational Diabetes
Test Mnemonic: GTOB
Test Includes: Fasting, 1 hr, 2 hr, 3 hr glucose
Lab Performing Test: Chemistry (Call for instructions)
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must fast for Minimum 8 hrs - not longer than 14
hrs. Fasting is drawn then a 100gm dose oral glucose is given
Special Instructions: Overnight fast. NPO until test completed
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum each timed collection
Availability: Daily 7AM-llPM
Time Required Routinely for Processing: 8 hours
Reference Range: See Report
Glucose Tolerance Test 2 Hours
Synonyms: GTT
General Use: Diabetes
Test Mnemonic: 2 hr: GTT2
Test Includes: Glucose determination at fasting and 2 hours.
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must fast at least 8 hrs and not more than 14 hrs.
NPO until test is completed. Fasting is drawn then patient is given a 75 gm dose
of oral glucose. Blood specimen is drawn at 2 hours.
Specimen Required: Clotted blood (Serum)
Container Required: Red top (SST) for each timed collection and FBS
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1 ml serum each collection
Availability: Daily
Time Required Routinely for Processing: 8 hours
Additional information: The standard test is 2 hrs with a 75gm oral glucose
dose.
Reference Range: See report
Glycosylated Hemoglobin
Synonyms: Hemoglobin A1c,
General Use: To aid in the management of diabetic patients
Test Mnemonic: A1C
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Lavender top tube (may be shared with CBC tube)
Volume of Specimen: 5 ml blood
Minimum Volume of Specimen: 2 ml whole blood
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Contraindications: Not useful more often than at 4-6 week intervals. Not
indicated for diagnosis of diabetes.
Additional information: Chronic blood loss, hemolytic anemia, or other setting
for decrease in RBC life span, results in a decrease in the glycosylated
hemoglobin level.
Reference Range: 4.2-6.3%
Diabetics out of control: greater than 12%
HCG, male
Synonyms: Human chorionic gonadotropin
General Use: This marker may be useful in diagnosis, therapy and prognosis of
testicular embryonal carcinoma
Test Mnemonic: HCG
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: <2.0 mIU/mL
HCG, Quantitative, Beta Subunit
Synonyms: Quantitative Bhcg
General Use: Diagnosis of certain cancers such as choriocarcinoma and certain
embryonal cell carcinomas as well as the detection of ectopic pregnancy
Test Mnemonic: BHCG
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Not done on urine. Gross hemolysis
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: Reference ranges for normal gestational weeks are provided
with test result
Heart Profile
Synonyms: MI Profile
General Use: Cardiac evaluation for MI
Test Mnemonic: HEART
Test Includes: Total CPK and Troponin I
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen:10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Daily 24 hrs
Time Required Routinely for Processing: Routine 8 hours; 1 hour Stat
Reference Range: See individual reports
Hemoglobin, Total
Synonyms: tHb
Test Mnemonic: THGB
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Notify lab if on anticoagulants
Specimen Required: Whole Blood (heparinized)
Container Required: Heparinized syringe, green top tube
Volume of Specimen: 3 ml
Minimum Volume of Specimen: 85 uL
Availability: Stat 24 hours per day
Time Required Routinely for Processing: 30 minutes
Reference Range: See report
Hepatitis A Antibody (IgM)
General Use: Presence of Hepatitis A Antibody (lgM) indicates recent acute
phase infection with Hepatitis A virus
Test Mnemonic: HAVABM
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon – Fri 7:00AM – 5:00PM, Sat 7:00am – 3:00 pm
Time Required Routinely for Processing: 24 hrs
Additional information: May be ordered as part of Hepatitis Panel (HEPP)
Reference Range: Reported as Positive or Negative
Hepatitis B Core Antibody (IgM)
Synonyms: HBc Ab
General Use: This marker is an early indicator of acute infection with Hepatitis B
virus
Test Mnemonic: HBCIGM
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon – Fri 7:00AM – 5:00PM; Sat 7:00AM – 3:00PM
Time Required Routinely for Processing: 24 hours
Additional information: May be ordered as part of Hepatitis Panel (HEPP)
Reference Range: Reported as positive or negative
Hepatitis B Surface Antibody
Synonyms: Hepatitis Bs Ab, HBs Ab
General Use: Screening test for past infection and subsequent immunity to
Hepatitis B Virus
Test Mnemonic: HBSAB
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red Top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Excessive hemolysis, chylous serum
Availability: Mon – Fri 7:00AM – 5:00PM; Sat 7AM – 3PM
Time Required Routinely for Processing: 24 hours
Reference Range: Reported as Positive or Negative
Hepatitis B Surface Antigen
Synonyms: HBsAg
General Use: Presence of HBsAg antigen indicates present infection with
Hepatitis B virus or chronic carrier state
Test Mnemonic: HBSAG
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon – Fri 7:00AM – 5:00PM; Sat 7AM-3PM; STAT upon request
Time Required Routinely for Processing: 24 hrs
Additional information: May be ordered as part of Hepatitis Panel (HEPP)
Reference Range: Negative
Hepatitis C Virus Antibody
General Use: Used to aid in the diagnosis of Hepatitis C infection
Test Mnemonic: HEPC
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon – Fri 7:00AM – 5:00PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hrs
Additional information: May be ordered as part of Hepatitis Panel (HEPP)
Reference Range: Negative
Hepatitis Panel
General Use: Screening for infection with Hepatitis A, Hepatitis B, or Hepatitis
C virus
Test Mnemonic: HEPP
Test Includes: HbsAg, HbcAb (IgM), Hepatitis A Antibody (IgM), Hepatitis C
Virus Ab.
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red Top Tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 3 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon – Fri 7:00AM – 5:00PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hrs
Reference Range: Individual tests reported as positive or negative
HIV-1 Antibody
Synonyms: Human lmmunodeficiency Virus, AIDS test
General Use: Used to support a diagnosis of AIDS or AIDS related complex
Test Mnemonic: HIV
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon – Fri 7:00am-5:00pm; Sat 7:00am-3:00pm, Stat available upon
request
Time Required Routinely for Processing: Stat 2 hrs/Routine 24 hrs. If positive,
the Western Blot test is done for confirmation
Additional information: This test is designed to help detect infection by or
exposure to the virus which as been implicated as the cause of Acquired
Immunodeficiency Syndrome (AIDS)
Reference Range: Negative
Homocysteine
General Use: Elevated levels of homocysteine are observed in patients at risk for
coronary heart disease and stroke.
Test Mnemonic: HCYS
Lab Performing Test: Chemistry
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Fasting for at least 8 hours is preferred
Special Instructions: Put specimen on ice immediately after collection.
Centrifuge within 1 hour. Keep refrigerated.
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Specimen received at room temperature
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 2 hrs
Reference Range: See Report
IgE
Synonyms: Immunoglobulin E
General Use: Management of patients with atopic disease such as asthma, hay
fever, eczema, and in patients with respiratory allergies
Test Mnemonic: IGE
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis. chylous serum
Availability: Mon – Fri 7am-3pm
Time Required Routinely for Processing: 48 hrs
Reference Range: See report
Immunoglobulin Panel
General Use: To evaluate humoral immunity or monitor therapy in myeloma
patients. Maybe of diagnostic importance in infections and autoimmune diseases
Test Mnemonic: IMMGS
Test Includes: IgG, IgA and IgM
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis or chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs: Stat 1 hour
Reference Range: Reference ranges are age dependent - see report
Infectious Mononucleosis Test
Synonyms: Monospot Test
General Use: For the detection of heterophile antibodies related to infectious
mononucleosis
Test Mnemonic: MONOS
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours a day, Mon-Sun.; Stat and routine
Time Required Routinely for Processing: Routine 8 hrs; STAT 1 hr
Reference Range: Negative
Insulin Glucose Tolerance, 2 or 3 Hours
Synonyms: IGT
General Use: Insulin to Glucose levels have prognostic value in predicting the
benefits of insulin therapy and likelihood of progression to insulin-dependence
Test Mnemonic: IGT2: 2 hour; IGT3: 3 hour
Test Includes: Insulin and Glucose determinations at fasting at 1/2 hour and for
each hour of the tolerance ordered
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Overnight fasting. NPO until test is completed
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST) for each timed collection and FBS
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum each timed collection
Availability: Mon.- Fri 7:00am – 11:00pm;Sat. 7AM-3PM
Time Required Routinely for Processing: 8 hrs.
Reference Range: See report
Insulin, Random
General Use: Hypoglycemia, pancreatic function, insulin resistance
Test Mnemonic: INS
Lab Performing Test: Chemistry;
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Mon –Fri 7:00am-5:00pm; Sat 7AM-3PM
Time Required Routinely for Processing: 1 day
Reference Range: See report
Iron Profile
General Use: Evaluation of iron metabolism; differential diagnosis of anemia
Test Mnemonic: IRON
Test Includes: Serum iron, Total iron binding capacity and % Transferrin
Saturation
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Additional information: Indicate whether or not patient is receiving Iron therapy
Reference Range: See individual test reports
Lactic Acid, Blood
Synonyms: Lactate
General Use: Evaluate lactic acidosis; metabolic acidosis
Test Mnemonic: LACT
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should be fasting and at rest.
Special Instructions: Specimen must be delivered on ice to laboratory
immediately after collection.
Specimen Required: Whole venous blood
Container Required: Gray top tube, place tube on ice!
Volume of Specimen: 7 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Specimen not kept on ice or delay in transport to Lab
Availability: 24 hrs daily
Time Required Routinely for Processing: Stat - 1 hour
Reference Range: 0.4-2.0 mmol/L.
Lactic Acid, CSF
Synonyms: CSF Lactate
General Use: Diagnosis of disorders of the central nervous system
Test Mnemonic: CSFLA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Spinal fluid
Container Required: Sterile glass/plastic tube
Volume of Specimen: 1.0 ml
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: 0.6-2.2 mmol/L
Lactic Dehydrogenase, Blood
Synonyms: LDH
General Use: Cardiac and liver disorder; certain tumors
Test Mnemonic: LDH
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: 87-241 U/L
Lactic Dehydrogenas, Fluid
General Use: Evaluation of hemolytic states
Test Mnemonic: FLLDH
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate source when ordering
Container Required: Sterile glass/plastic tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml
Causes for Rejection: Viscous or clotted specimen
Availability: 24 Hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hr
Reference Range: Reference range not established
Lactose Tolerance Test
Synonyms: Lactose consumption test
General Use: Lactose intolerance evaluation.
Test Mnemonic: LTOL
Test Includes: Fasting, 15 min., 30 min., 60 min., and 90 min. lactose
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: NPO after midnight preceding day of test.
Special Instructions: A fasting specimen must be drawn before lactose is given
to patient. Coordinate lactose administration and specimen collection with
Phlebotomy team. Specimens drawn 15, 30, 60 and 90 minutes post Lactose
Dose.
Specimen Required: Whole venous blood
Container Required: Gray top tube
Volume of Specimen: 7 ml
Minimum Volume of Specimen: 7 ml
Availability: Monday-Friday, 7AM to 3PM. Not Stat
Time Required Routinely for Processing: 8 hours
Additional information: Lactose is obtained from pharmacy by nursing for
inpatients; by laboratory for outpatients
Reference Range: Interpretation: An increase of blood glucose >20 mg/dL over
the fasting level on any of the timed specimens is considered normal (based on a
50gm lactose date)
Latex Allergen
Synonym: Allergy testing/K82
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: K82
Test Includes: Allergy screening for latex.
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Special Instructions: none
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon-Fri 7am to 3pm
Time Required Routinely for Processing: 48 hrs
Reference Range: Interpretation provided with results.
LDL cholesterol
Synonyms: LDL, direct
General Use: Determination of potential risk for heart disease
Test Mnemonic: LDLD
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours
Reference Range: See Report
Lipase, Serum
Synonyms: Lipase
General Use: Pancreatic function
Test Mnemonic: LIPS
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 73-393 u/L
Lithium, Blood
Synonyms: Li
General Use: Therapeutic Drug monitoring
Test Mnemonic: LI
Lab Performing Test: Chemistry lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours: Stat 1 hour
Reference Range: Therapeutic range 0.6-1.2 mmol/L
Liver Profile
Synonyms: Liver function tests
Test Mnemonic: LIV
Test Includes: TPRO, TBlL, DBIL, ALKP, ALT, AST,ALB and globulin
Lab Performing Test: Chemistry lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Daily 24 hrs
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See individual test reference ranges
Luteinizing Hormone
General Use: Assessment of hypothalamic function and pituitary function; to
distinguish between primary and secondary gonadal failure
Test Mnemonic: LH
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Excessive hemolysis, chylous serum
Availability: Mon-Fri 7AM to 5PM; Sat 7AM to 3PM.
Time Required Routinely for Processing: 24 hours
Reference Range: See report. Ranges are sex and cycle dependent
Lyme Disease Serology, Serum
Synonyms: Lyme Arthritis Antibodies
General Use: To aid in the diagnosis of Lyme disease
Test Mnemonic: LYME
Test Includes: Lyme Abs IgG and IgM
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Excessive hemolysis
Availability: Mon-Fri 7am to 5pm; Sat 7am -3pm
Time Required Routinely for Processing: 24 hrs
Limitations: Positive or borderline results will be referenced out for Western blot
testing
Reference Range: Negative
Magnesium, Blood
Synonyms: Mg
General Use: Evaluation of metabolic disorders
Test Mnemonic: MG
Lab Performing Test: Chemistry Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Rourine 8 hrs; stat 1 hour
Reference Range: 1.8 - 2.4 mg/dL
Measles Immunity Panel
General Use: To determine immunity to Rubella and Rubeola (measles virus)
Test Mnemonic: MIMP
Test Includes: Rubella immune status, Rubeola immune status
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Mon – Fri 7am-5pm; Sat 7am-3pm
Time Required Routinely for Processing: 2-3 days
Reference Range: Reported as immune or non-immune
Methemoglobin
General Use: Evaluation of cyanosis or methemoglobinemia due to drugs or
chemicals
Test Mnemonic: METHGB
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (heparinized) * Must be transported on ice
Container Required: Heparinized syringe or Green top tube (Na Heparin)
Volume of Specimen: 3 ml
Minimum Volume of Specimen: 85 uL
Causes for Rejection: Specimen not on ice when received
Availability: 24 hrs. daily
Time Required Routinely for Processing: 1 hour
Reference Range: See report
Microalbumin, Urine
Synonyms: Urinary Albumin
General Use: Evaluation of renal function. Diagnosis of diabetic nephropathy
Test Mnemonic: Random specimen: UMCALM; 24 hr collection; UMCA24
Test Includes: Microalbumin and urine creatinine
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: 24 hr collection or random urine
Container Required: 24 hr urine container obtained from chemistry; yellow top
urine tube or random urine cup
Volume of Specimen: Entire collection for 24 hour/10 ml for random
Minimum Volume of Specimen: Entire collection for 24 hour/2 ml for random
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hrs: Stat 1 hour
Reference Range: See Report
New England Regional Inhalant Panel
Synonyms: Allergy Testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: RIPNE
Test Includes: Screening test for Northeast regional trees, grasses, molds and
animal allergens. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml serum
Availability: Mon-Fri 7AM to 3PM
Time Required Routinely for Processing: 48 hrs
Additional information: A complete listing of the allergens included is available
from the lab. Call 444-5119
Reference Range: Interpretation provided with test results
Nut Panel
Synonym: Allergy testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: NUTP
Test Includes: Allergy screening for peanut, hazelnut, brazil nut, almond, pecan,
pistachio, cashew and walnut. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon-Fri 7am to 3pm
Time Required Routinely for Processing: 48 hrs
Reference Range: Interpretation provided with results.
Osmolality, Serum
Synonyms: Serum Osmolality
General Use: Dehydration, electrolyte balance
Test Mnemonic: OSMO
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 0.5 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 275-295 mOsm/Kg
Osmolality, Urine
Test Mnemonic: UOSM
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Random urine
Container Required: Yellow top urine tube or urine container routinely used
for random collection
Volume of Specimen: 5 ml
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 450-750 mOsm/Kg
PG - FLM Test
Synonyms: Phosphotidyglycerol Slide Test
General Use: Fetal lung maturity determination
Test Mnemonic: PGFLM
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Amniotic fluid from amniocentesis or vaginal pool
Container Required: Dark brown sterile plastic tube
Volume of Specimen: 2 ml
Minimum Volume of Specimen: 1 ml amniotic fluid
Availability: Mon-Fri 7AM to 11PM; Sat 7AM to 3PM; STAT upon request,
Call lab 444-5119
Time Required Routinely for Processing: 1 hour
Reference Range: Positive indicates maturity
pH, Body Fluid
Test Mnemonic: FPHP
General Use: Determine pH of clinical specimen.
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate source when ordering
Specimen Required: Body fluid (urine, gastric, thoracentesis, etc.)
Container Required: Sterile glass/plastic tube
Volume of Specimen: 2 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Viscous or clotted specimen
Availability: Daily 7AM-11PM
Time Required Routinely for Processing: 8 hours
Reference Range: Reference range not established
Phenobarbital, Blood
Synonyms: Phenobarb level
General Use: Monitor therapeutic drug level
Test Mnemonic: PHEN
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Additional information: Optimal sampling time after dosage is 2-8 hrs.
Reference Range: Therapeutic level: 15-40 ug/mL; Toxic level: > 50 ug/mL
Phosphate, Urine
Synonyms: Urinary Phosphorus
General Use: Electrolyte disorders; renal function test
Test Mnemonic: Random specimen: UPHOS; 24 hr collection: UPHS24
Test Includes: Phosphorus on random or timed urine specimen
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Urine specimen obtained by nursing staff for Inpatients
Specimen Required: Timed or random urine
Container Required: 24 hour urine container supplied by laboratory; Yellow top
urine tube or urine cup for random testing
Volume of Specimen: Entire specimen for 24 hr collection/ 10 ml for random
Minimum Volume of Specimen: Entire specimen for 24 hour/1 ml for random
Availability: Daily 7AM-11PM
Time Required Routinely for Processing: 1 Day
Reference Range:
24 hr specimens: 0.3-1.0 g/TV (dependent on dietary
intake)
Random: Ranges not established, dependent on diurnal variation
Phosphorus, Blood
Synonyms: P04
General Use: Evaluation of phosphorus metabolism
Test Mnemonic: PHOS
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml.
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 2.5-4.9 mg/dL
Potassium, Blood
Synonyms: K
General Use: Evaluate electrolyte balance
Test Mnemonic: K
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 0.5 ml serum
Causes for Rejection: Moderate to gross hemolysis; drawn from an IV infused
vein
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 3.5-5.1 mmol/L
Potassium, Urine
Synonyms: K, Urine
General Use: Renal function
Test Mnemonic: 24hr: UK24; Random Urine: UK
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Random or 24 hr urine collection
Specimen Required: Random or timed urine (24hr)
Container Required: routine urine container or yellow top urine tube; 24 hour
urine container obtained from Chemistry
Volume of Specimen: Entire collection or 1 ml for random
Minimum Volume of Specimen: Entire timed collection
Availability: Daily, 7AM-11PM
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range:
24 hr specimens: 25-100 mEq/24 hr
Random: 30-90 mEq/L (Varies with diet)
Prealbumin
Synonyms: Transthyretin, Thyroxine-binding prealbumin
General Use: Screening for protein-calorie malnutrition and to monitor the
effectiveness of nutritional support therapy
Test Mnemonic: PAB
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs: Stat 1 hour
Reference Range: 20-40 mg/dL
Pregnancy Test, Serum
Synonyms: Bhcg, Qualitative
General Use: Screening test for pregnancy
Test Mnemonic: PREGS
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: 24 hours a day, Mon-Sun. STAT and routine
Time Required Routinely for Processing: Routine 8 hours; STAT 1 hour
Reference Range: Non-pregnant females: Negative
Normal pregnant female: Positive
Pregnancy Test, Urine
General Use: Screening test for pregnancy
Test Mnemonic: UPREG
Lab Performing Test: Serology/Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Urine specimen, preferably first morning void
Container Required: Clean sterile urine cup or yellow top urine tube
Volume of Specimen: 2 ml
Minimum Volume of Specimen: 1 ml urine
Availability: 24 hrs a day, Mon-Sun. STAT and routine
Time Required Routinely for Processing: Routine 8 hours; STAT 1 hour
Reference Range: Non-pregnant females: Negative
Normal pregnant female: Positive
Prenatal Profile
General Use: Prenatal screening
Test Mnemonic: PRENAT 1
Test Includes: BsAg, Syphab, Rubella, CBC, Ab Screen, ABO/Rh,A1C and HIV
Lab Performing Test: Chemistry, Hematology, Blood Bank
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Must be ordered as order set
Specimen Required: Clotted blood and whole blood (EDTA)
Container Required: Red top tube(SST), Lavendar (EDTA) and K2EDTA Pink
Top
Volume of Specimen: 10 ml clotted Blood, 4 ml Lavender EDTA and 6 ml
K2EDTA
Minimum Volume of Specimen: 3 ml Serum, 3 ml Lavender EDTA Blood and 2
ml K2EDTA Blood
Availability: 7AM-11PM; Mon-Sun. Not stat
Time Required Routinely for Processing: 1-4 days
Reference Range: See individual tests for reference ranges
Procalcitonin
General Use: To aid in the work-up of infectious causes of sepsis.
Test Mnemonic: PROCAL
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis
Availability: 24 Hrs daily
Time Required Routinely for Processing: 1 Hour
Reference Range: See text report
Progesterone
General Use: As an indicator of natural or induced ovulation or in the diagnosis
of threatened abortion
Test Mnemonic: PROG
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis or chylous serum
Availability: Mon - Fri 7AM-5PM; Sat 7am-3pm May be ordered STAT
Reference Range: Ranges vary according to sex and cycle of female – See test
report.
Prolactin
General Use: Pituitary function test useful in the detection of prolactin secreting
pituitary tumors with or without galactorrhea and in the assessment of pituitary
dysfunction
Test Mnemonic: PROL
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Excessive hemolysis, chylous serum
Availability: Mon-Fri 7AM-5PM; Sat 7AM-3PM
Time Required Routinely for Processing: 24 hours
Reference Range: see report
Prostate Specific Antigen
Synonyms: PSA
General Use: To aid in the diagnosis and monitoring of Prostatic Ca and BPH
Test Mnemonic: PSA
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis or chylous serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; STAT 1 hour
Reference Range: <4.0 ng/ml
Protein, Body Fluid
General Use: Evaluating pathological processes
Test Mnemonic: FTOTP
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Body fluid (i.e. ascites fluid, pleural fluid, etc.)
Container Required: Clean glass or plastic tube
Volume of Specimen: 3 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Viscous or clotted specimen
Availability: 24 Hrs daily
Time Required Routinely for Processing: 8 hours
Additional information: Identify source of body fluid in comment field
Reference Range: Reference ranges not established
Protein, CSF
General Use: Diagnosis of CSF pathological processes
Test Mnemonic: CSFTP
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Physician's responsibility
Special Instructions: Specimen must be brought to laboratory Stat
Specimen Required: Cerebrospinal fluid
Container Required: Any sterile container
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 2 hours; Stat 1 hour
Additional information: Fresh blood in the specimen will invalidate the result;
xanthochromia or hemolysis may falsely depress results
Reference Range: 15-45 mg/dL
Protein, Total, Blood
Synonyms: TP
General Use: Evaluate serum proteins, nutritional status, liver and renal function
Test Mnemonic: TPP
Test Includes: Total Protein, Albumin, Globulin
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: See individual tests
Protein, Urine
General Use: Detection of proteinuria
Test Mnemonic: Random: UTP and 24 hr: UTP24
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: 24 hr: instruct the patient to void at 8:00 AM and discard
the specimen. Then collect all urine including the final specimen voided at the end
of the 24 hour collection period.
Specimen Required: Timed or random urine
Container Required: Random urine container or yellow top urine tube/ 24 hour
urine container obtained from chemistry
Volume of Specimen: 24 hr collection: entire specimen; random collection 10 ml
Minimum Volume of Specimen: 24 hr collection : entire specimen: random
collection : 1 ml
Availability: Daily, 7AM-11PM
Time Required Routinely for Processing: 24 hours
Additional information: Exact hours of specimen collection should be recorded
on label supplied with bottle. 24-hour collections are preferable for evaluation of
nephrotic states and inflammatory renal disorders
Reference Range: 24 hr specimens: 0-0.15 gm/TV Random: 0-14 mg/dL
PTH, intact panel
Synonym: Intact parathyroid hormone
General Use: Aid in the differentiation of hyperparathyroidism from nonparathyroid hypercalcemia
Test Mnemonic: PTHP
Test Includes: PTH, intact and Calcium
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum) and whole blood (plasma)
Container Required: Red top (SST) and Lavender top (EDTA)
Volume of Specimen: 10 ml clotted blood and 5 ml whole blood
Minimum Volume of Specimen: 1 ml serum and 1 ml plasma
Causes for Rejection: Improper container, gross hemolysis
Availability: Monday – Friday 7 am to 5 pm; Sat 7am-3pm. STAT upon request
Time Required Routinely for Processing: Routine 8 hours, STAT 1 hour
Reference Range: See report for interpretation
RAST testing – miscellaneous tests
Synonym: Allergy testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: Order as misc test
Available misc tests: Tomato, orange, tuna, salmon, apple, casein, kiwi, banana,
fusarium proliferatum, cow’s whey, mountain cedar, elm, walnut tree,
cottonwood, mulberry, sheep sorrel. Please contact specimen processing for any
allergen not listed, ext. 3139.
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: Varies depending on number of tests ordered
Causes for Rejection: Gross hemolysis
Availability: Mon-Fri 7am to 3pm
Time Required Routinely for Processing: 48 hrs
Additional Information: Latex, New England Regional Inhalant Panel,
Comprehensive Inhalant Panel, General Food Screen, Shellfish Panel, Nut Panel,
Childhood Allergy Panel, and Stinging Insect Panel are orderable and are listed
separately in the lab guide.
Reference Range: Interpretation provided with results.
Rheumatoid Factor
Synonyms: RAF Titer
General Use: Diagnostic test for Rheumatoid arthritis
Test Mnemonic: RF
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: < 15.0 IU/mL
Rheumatoid Profile
Synonyms: RA Profile
General Use: Screening tests for SLE, Rheumatoid Arthritis, Gout, Lyme,
arthritis.
Test Mnemonic: RHEU
Test Includes: ANA, RA factor, Lyme titer, Uric acid, CBC, Sed rate
Lab Performing Test: Hematology & Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (serum) and whole blood (EDTA)
Container Required: Red top tube, Lavender (EDTA) tube, Black top VacuTech tube
Volume of Specimen: 10 ml clotted blood, 5 ml whole blood
Minimum Volume of Specimen: 4 ml serum, 2 ml whole blood
Causes for Rejection: Gross hemolysis
Availability: 7:00AM-11:00PM; Mon. thru Sun. Not Stat.
Time Required Routinely for Processing: 3-5 days
Reference Range: see individual assays
Rubella, Status
Synonyms: German measles immunity titer
General Use: To determine immune status to Rubella virus
Test Mnemonic: RUBL
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon - Fri 7AM-llPM. Sat 7AM-3PM
Time Required Routinely for Processing: 24 hrs
Reference Range: Reported as Immune or Non-immune
Rubeola, Status
Synonyms: Rubeola, immunity
General Use: To determine immunity to or past exposure to Rubeola virus
Test Mnemonic: RUBE
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood
Container Required: Red top tube
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis. chylous serum
Availability: Mon - Fri 7AM-11PM. Sat 7AM-3PM
Time Required Routinely for Processing: 2-3 days
Reference Range: Reported as Immune or Non-immune
Salicylate, Blood
Synonyms: Aspirin level
General Use: Monitor therapy; toxicology
Test Mnemonic: SAL
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine: 8 hours; stat, 1 hour
Reference Range: Therapeutic: 2.8 – 20.0 mg/dL; >30 mg/dl potentially toxic
result
SGOT/AST
Synonyms: GOT, AST
General Use: Liver evaluation
Test Mnemonic: AST
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 15-37 U/L
SGPT/ALT
Synonyms: GPT, ALT
General Use: Liver evaluation
Test Mnemonic: ALT
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted serum (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 12-78 U/L
Shellfish Panel
Synonym: Allergy testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: SHELLP
Test Includes: Allergy screening for crab, shrimp, blue mussel, lobster, clam,
oyster and scallop. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Special Instructions:
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml serum
Causes for Rejection: Gross hemolysis
Availability: Mon-Fri 7am to 3pm
Time Required Routinely for Processing: 48 hrs
Reference Range: Interpretation provided with results.
Sodium, Blood
Synonyms: Na
Test Mnemonic: NA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 136-145 mmol/L
Sodium, Urine
Synonyms: Urine Na
General Use: Renal function
Test Mnemonic: UNA: random; UNA24: 24 hour collection
Lab Performing Test: Chemistry
Request Form: Universal requisition: used only as back-up to Meditech
Specimen Required: Random or 24 hr collection
Container Required: 24 hour urine container supplied by laboratory; Yellow top
urine tube or urine cup for random
Volume of Specimen: Entire collection
Minimum Volume of Specimen: 0.5 ml
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range:
24 hr specimens: 80-290 mEq/24 hr
Random: Ranges not established
Stinging Insect Panel
Synonyms: Allergy Testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: SIP
Test Includes: Screening test for honeybee venom, white faced hornet, yellow
jacket venom, paper wasp venom and yellow hornet. Total IgE is included with
panel.
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Availability: Mon-Fri 7AM to 3PM
Time Required Routinely for Processing: 48 hrs
Reference Range: Interpretation provided with results
Syphyllis Antibodies, Blood
Synonyms: Rapid Plasma Reagin test
General Use: Screening test for syphilis, Prenatal blood testing
Test Mnemonic: SYPHAB
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Excessive hemolysis, chylous serum
Availability: Mon-Fri 7am-5pm, Sat 7am – 3pm
Time Required Routinely for Processing: 72 hours. Positive and borderline
tests are referenced out for RPR confirmatory testing.
Reference Range: Nonreactive
T-3, Total
Synonyms: Triiodothyronine
General Use: Thyroid function, specifically used in the diagnosis of T-3
thyrotoxicosis
Test Mnemonic: TT3
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml of serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Additional information: Not to be confused with T3 Uptake which is part of
Thyroid Profile
Reference Range: See report
T4, Free
Synonyms: Free Thyroxine, FT4
General Use: Test for thyroid function
Test Mnemonic: FT4
Lab Performing Test: Chemistry/Serology
Request Form: Universal requisition; use only as a back up to Meditech
Specimen Required: Clotted blond (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range : See report
Tegretol
Synonyms: Carbamazepine
General Use: Monitor therapeutic drug level
Test Mnemonic: TEG
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 1.0 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hr
Reference Range: Therapeutic: 8-12 ug/mL ; > 15 ug/mL is potentially toxic
Testosterone, Total
General Use: This test is useful in the diagnosis of hypogonadism in males and
hirsutism and virilization in females
Test Mnemonic: TEST
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml serum
Availability: Mon-Fri 7:00am – 11:00pm; Sat 7:00am-3:00pm
Time Required Routinely for Processing: 24 hrs
Reference Range: See report
Theophylline
Synonyms: Aminophylline
General Use: Monitor therapeutic drug level
Test Mnemonic: THEO
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted Blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours, Stat 1 hour
Reference Range: Therapeutic: 10-20 ug/ml ; > 20 ug/mL potentially toxic
Thyroid Profile
General Use: Thyroid function assessment and monitoring
Test Mnemonic: THY
Test Includes: T3 Uptake, T4, TSH ultra and calculated Free Thyroxine Index
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: See report
Thyroid Stimulating Hormone, Ultra sensitive
Synonyms: TSH Ultra
General Use: Differential diagnosis of primary hypothyroidism from secondary
hypothyroidism
Test Mnemonic: TSH
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: See report
Tissue Transglutaminase IgA
Synonym: IgA tTg
Test Mnemonic: TTGIGA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Mon - Fri 7:00am-3:00pm
Time Required Routinely for Processing: 48 hours
Reference Range: See Report
Tree Nut Panel
Synonym: Allergy testing
General Use: To aid in the diagnosis of atopic allergies
Test Mnemonic: TRNUTP
Test Includes: Allergy screening for hazelnut,brazil nut, almond,pecan, pistachio
Cashew and walnut. Total IgE is included with panel.
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: none
Specimen Required: clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 5 ml of serum
Causes for Rejection: Gross hemolysis
Availability: Mon-Fri 7am to 3pm
Time Required Routinely for Processing: 48 hrs
Reference Range: Interpretation provided with results
Tobramycin
Synonyms: Nebcin
General Use: Monitor therapeutic level
Test Mnemonic: Trough: TOBT; peak: TOBP; random: TOBR
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Peak level: 30 minutes after IV infusion is completed;
Trough: immediately before next dose.
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: Daily 24 hrs
Time Required Routinely for Processing: 2 hours
Additional information: Levels should be drawn at steady state, usually 24-36
hours after starting treatment, depending on dosing schedule.
Reference Range: Therapeutic Peak: 4-8 ug/mL; Trough: 0-2 ug/mL
Triglycerides, Blood
Synonyms: Trig
Test Mnemonic: TRIG
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: 12-14 hrs. fasting; should be on stable diet 2 weeks prior to
collection of blood
Specimen Required: Clotted blood (Serum)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml of serum
Container Required: Red top tube (SST)
Lab Performing Test: Chemistry
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Causes for Rejection: Nonfasting sample
General Use: Evaluate hyper or hypo lipidemia
Reference Range: <150 mg/dL
Triglycerides – Fluid
Test Mnemonic: FTRIGS
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate source when ordering
Container Required: Sterile glass/plastic tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml
Causes for Rejection: Viscous or clotted specimen
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: Reference ranges not established
Troponin I
Synonym: Cardiac Troponin
General Use: Assessment of AMI
Test Mnemonic: TROPIP
Lab Performing Test: Chemistry
Request Form: Universal Requisition; use only as a backup to Meditech
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 0.5 ml serum
Causes for Rejection: Improper tube type
Availability: 24 hrs. daily
Time Required Routinely for Processing: 1 hour STAT; Routine 8 hrs
Additional information: May also be ordered as part of MI profile (HEART)
Reference Range: See report
Urea Nitrogen, Blood
Synonyms: BUN; Blood Urea Nitrogen
General Use: Kidney function
Test Mnemonic: BUN
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hr
Reference Range: 7-18 mg/dL
Urea, Urine
Synonyms: Urinary Urea Nitrogen
General Use: Kidney function
Test Mnemonic: Random: UUN; 24 hr collection UUN24
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Refrigerate urine during collection
Specimen Required: Random or 24 hour urine
Container Required: 24 hour urine container obtained from Chemistry; yellow
top urine tube or urine cup for random collection
Volume of Specimen: 24 hr collection : Entire collection; random collection :10
ml
Minimun Volume of Specimen: 24 hr collection: entire collection ; random
collection : 1 ml
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hr
Reference Range:
24 hr specimens: 6-17 gm/TV
Random: Ranges not established
Uric Acid, Blood
Synonyms: UA
General Use: Diagnosis of gout, metabolic disorders, and toxemia of pregnancy
Test Mnemonic: URIC
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hour
Reference Range: 2.6-6.0 mg/dL females; 3.5-7.2 mg/dL males
Uric Acid, Fluid
General Use: Diagnosis of gout and rheumatoid syndromes
Test Mnemonic: FURICA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate source when ordering
Container Required: Clean glass/plastic tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 0.5 ml
Causes for Rejection: Viscous or clotted specimen
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: Reference ranges not established
Uric Acid, Urine
Synonyms: Urinary UA
General Use: Uric acid metabolism
Test Mnemonic: Random: UUA; 24 hr collection: UUA24
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Normal diet 24 hours prior to collection
Special Instructions: Refrigerate urine during collection
Specimen Required: Random or 24 hr. collection
Container Required: 24 hour urine container obtained from chemistry; urine
cup for random collection or yellow top urine tube
Volume of Specimen: 24 hr collection : entire collection ; random collection : 10
ml
Minimum Volume of Specimen: 24 hr collection : entire collection ; random
collection : 1 ml
Availability: 24 hours daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range:
24 hr specimens: 0.25 - 0.75 gm/TV
Random: Reference ranges not established
Valproic Acid
Synonyms: Depakene
General Use: Monitor therapeutic level
Test Mnemonic: VPA
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Trough values drawn just before next dose possibly more
useful than peak values drawn 1-3 hours after an oral dose.
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hrs; Stat 1 hour
Reference Range: Therapeutic range: 50-100 ug/mL; > 120 ug/mL potentially
Toxic
Vancomycin
General Use: Therapeutic drug monitoring
Test Mnemonic: Trough level: VANT; peak level: VANP
Lab Performing Test: Chemistry
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml serum
Collection: Trough levels are collected 1/2 hr before dose is given. Peak levels
are collected 1 hr after infusion is finished
Availability: 24 hrs daily
Time Required Routinely for Processing: Routine 8 hours; Stat 1 hr
Reference Range: Trough: 5-10 ug/mL; Peak levels: 30 min post 1hr infusion:
30-40 ug/mL, 60 min post 1 hr infusion: 25-40 ug/mL, 120 min post 1 hr infusion:
18-26 ug/mL
Vitamin B12
Synonyms: Cyanocobalamin, B12
General Use: Screening for B-12 deficiency, malabsorption; pernicious anemia
Test Mnemonic: B12
Lab Performing Test: Chemistry/Serology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (Serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml blood
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Gross hemolysis, chylous serum
Availability: Mon - Fri 7AM to 11PM; Sat 7AM to 3PM
Time Required Routinely for Processing: 24 hrs
Reference Range: 239-931 pg/ml
Vitamin D 25-Hydroxy
General Use: Measurement of serum 25-OH Vitamin D provides a good index of
circulating Vitamin D activity.
Test Mnemonic: VD25
Lab Performing Test: Chemistry/Serology
Request form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clotted blood (serum)
Container Required: Red top tube (SST)
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1.0 ml serum
Availability: Monday – Friday 7:00am-11:00pm,Sat 7:00am-3:00pm
Time Required Routinely for Processing: 1 day
Reference Range: See Report
COAGULATION
The Coagulation section of the Laboratory is directed by Victoria G. Reyes-D'Arcy/
Asim Ejaz, M.D., D.A.B. Pathologist, and Pedro Ugarelli, M.S., B.S., M.T., Hematology
Manager.
For procedures not listed please consult with the Hematology Manager(Ext. 4137) or
Pathologist.
All specimens must be properly labeled with patient's full name, identification number,
time and date of collection, and the test requested.
Activated Partial Thromboplastin Time
Synonyms: APTT
General Use: Measures intrinsic coagulation system; monitor unfractionated
heparin therapy
Test Mnemonic: APTT
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Container Required: Blue top tube (sodium citrate)
Specimen Required: Plasma
Volume of Specimen: 2.7ml
Minimum Volume of Specimen: 2.7ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen. Samples must be processed within 4
hours of collection
Availability: Routine and Stat 24 hours daily
Time Required Routinely for Processing: Routine: 2 hours; Stat: 30 minutes
Reference Range: See printed report
Antithrombin III
General Use: Part of hypercoagulation profile (HCOAG); quantitation of ATIII
level.
Test Mnemonic: ATIII
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml
Minimum Volume of Specimen: 2.7ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen. Samples must be processed within 4
hours of collection
Availability: Performed once a week
Reference Range: See printed report
APTT see Activated Partial Thromboplastin Time
C.A.C. Circulating Anticoagulant see: Mixing Studies
Coagulation Factor Assays see Factor VIII Assay
Coagulation Profile
Synonyms: Hemorrhagic Screen
General Use: Screening for coagulation disorder
Test Mnemonic: COAGP
Profile Includes: Prothrombin Time, APTT, Fibrinogen, Platelet Count, Platelet
Function Assay
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma and fresh whole blood
Container Required: 2 Blue top tubes (sodium citrate) and 1 lavender top tube
(EDTA)
Volume of Specimen: Two 2.7 ml (blue top tubes) and 4.0 ml of fresh whole
blood (lavender top tube)
Minimum Volume of Specimen: Two 2.7 ml (blue top tubes) and 4.0 ml whole
blood (lavender top tube)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours daily, routine and stat
Time Required Routinely for Processing: 2 hours
Reference Range: See printed report.
D-Dimer Quantative
Synonyms: Automated D-Dimer, Quantitative D-Dimer
General Use: Rule out DVT/PE in emergency room settings in low-moderate risk
patients.
Test Mnemonic: DDIMER
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Container Required: Blue top tube (sodium citrate)
Specimen Required: Plasma
Volume of Specimen: 2.7ml
Minimum Volume of Specimen: 2.7ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, specimen more than 2 hours old, mislabeled or unlabeled specimen.
Availability: Routine and Stat 24 hours daily
Time Required Routinely for Processing: Routine: 1-2 hours; Stat: 30 minutes
Reference Range: See printed report
D-Dimer Qualitative
Synonyms: DDimer Qualitative, DIM
General Use: Detection of fibrin degradation
Test Mnemonic: DDQUAL
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Whole blood
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml whole blood (blue top tube)
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours daily and Stat
Time Required for Processing: Routine same day, Stat 1 hour
Reference Range: Negative
DIC Profile
General Use: Assess intravascular clotting
Test Mnemonic: DIC
Profile Includes: Prothrombin Time (PT), APTT, Fibrinogen, Platelet Count, and
D-DQUAL
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: None (Note: anticoagulant therapy may interfere with test
result interpretation)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma and fresh whole blood.
Container Required: 2 Blue top tubes (sodium citrate) and 1 lavender top tube
(EDTA)
Volume of Specimen: Two 2.7 ml of plasma (blue top tubes) and 5.0 ml whole
blood (lavender top tube)
Minimum Volume of Specimen: Two 2.7 ml (blue top tubes) and 5.0 ml of fresh
whole blood (lavender top tube)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hrs, daily and STAT
Time Required Routinely for Processing: Routine same day; Stat 1 hour
Reference Range: See report.
Factor II Mutation see Prothrombin Gene Mutation
Factor VIII Assay
Synonyms: Factor 8 Assay, F8
General Use: Detecting specific coagulation factor assays.
Test Mnemonic: VIII
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Performed once a week
Reference Range: See printed report
Fibrinogen Level
General Use: Quantitative fibrinogen level
Test Mnemonic: FIBR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml (blue top tube)
Minimum Volume of Specimen: 1.2 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours routine and Stat.
Time Required Routinely for Processing: Routine 2 hours; Stat 30 minutes.
Reference Range: See printed report.
HCOAG see Hypercoagulable profile
Hemorrhagic Screen see Coagulation Profile
Hypercoagulable Profile
Synonyms: HCOAG
General Use: Assess hypercoagulable state
Test Mnemonic: HCOAG
Profile Includes: Protein C, ATIII, Factor VIII Assay, Fibrinogen, Lupus
Anticoagulant, Protein S, Prothrombin Time, APTT, and APCR.
Lab Performing Test: Hematology.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: 3 Blue top tubes (sodium citrate)
Volume of Specimen: 3 Full 2.7 ml blue top tubes
Minimum Volume of Specimen: 2.7 ml X 3
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Daily, not Stat; specimen collected and frozen 24 hours daily.
Time Required Routinely for Processing: 2-5 days
Reference Range: See printed report
LUPA see Lupus Anticoagulant
Lupus Anticoagulant
Synonyms: LA, LUPA
General Use: To evaluate patients for the presence of lupus anticoagulant
Test Mnemonic: LUPA
Lab Performing Test: Hematology send out
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2 Full 2.7 ml blue top tubes.
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Specimen collected and frozen 24 hours daily.
Time Required Routinely for Processing: Performed once a week
Reference Range: See report.
Mixing Studies
Synonyms: C.A.C. Coagulation Mixing studies, Mixing Studies
General Use: Detection of circulating antibodies
Test Mnemonic: MXSTDY
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: 2 full blue top tubes (sodium citrate)
Volume of Specimen: 2.7 mL
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Specimen collected and frozen 24 hours daily; test performed MonFri: 6am -10pm.
Time Required Routinely for Processing: Weekdays-same day; weekendsspecimen frozen and test performed on Mondays.
Additional Information Needed: Previous Prothrombin Time and APTT
Reference Range: See printed report.
PFA see Platelet Function Assay
Platelet Function Assay
General Use: To assess the ability of platelets to aggregate
Test Mnemonic: PFA
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Whole blood
Container Required: Glass Blue top tube (sodium citrate)
Volume of Specimen: 4.5 ml of whole blood (blue top tube)
Minimum Volume of Specimen: 4.5 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours daily
Time Required Routinely for Processing: 2 hours
Reference Range:
CEPI (Collagen/EPI): 65-181 seconds
ADP (Collagen/ADP): 53-113 seconds
Protein C
General Use: Quantitative Protein C level
Test Mnemonic: PC
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml (blue top tube)
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Test performed once a week.
Reference Range: See printed report
Protein S
General Use: Quantitative Protein S level
Test Mnemonic: PRS
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient must not be on anticoagulants
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml (blue top tube)
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Test performed once a week.
Reference Range: See printed report
Prothrombin Time/INR
Synonyms: PTBAT, Protime
General Use: Monitoring oral anticoagulants
Test Mnemonic: PTINR
Profile Includes: PT and INR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; do not use
butterfly needle. A discard tube must be drawn before a specimen for coagulation
testing; then, a citrated blue top tube(s) should be drawn next. If specimen is
drawn from an in-dwelling catheter, line must be flushed with saline and the first
10ml of blood collected must be discarded.
Specimen Required: Plasma
Container Required: Blue top tube (sodium citrate)
Volume of Specimen: 2.7 ml (blue top tube)
Minimum Volume of Specimen: 2.7 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen,
mislabeled or unlabeled specimen.
Availability: 24 hours daily and Stat
Time Required Routinely for Processing: Routine 2 hours; Stat 30 minutes
Reference Range: INR of 2.0-3.0 for standard prophylaxis
INR of 2.5-3.5 for high-risk prophylaxis
PT/INR see Prothrombin Time/INR
CYTOLOGY
The Cytology Section is under the direction of Anica Antic, M.D., Director of Cytology.
Services consist of examination of physiologically and artificially exfoliated or aspirated
cells. The section is staffed by two part-time cytotechnologists.
The Cytology Laboratory is open weekdays from 8:00 AM to 4:30 PM. After regular
hours, and on weekends, cytology specimens are placed in specimen refrigerators. Please
follow the appropriate protocol posted next to the Cytology door and on the refrigerator
door.
The most common sources of cytology specimens include gynecological smears and
liquid-based preparations such as sputum, bronchial, esophageal, gastric, urinary tract and
various body fluids (pleural and peritoneal) as well as fine needle aspirates from various
organs. The principle emphasis is the evaluation of cell populations for the detection of
malignant and pre-malignant changes.
Labeling of specimens: All specimens must be labeled in the presence of the patient and
submitted in a properly labeled container, to include the patient's name, date of birth, and
specimen type/source.
All prepared slides submitted must be identified by writing the patient's name, date of
birth and specimen source on the frosted end of the slide with a #2 or #3 lead pencil. For
all outpatient areas and the Emergency Department, each specimen, regardless of type,
must be submitted with a standard cytology requisition. The following information is
needed and must be entered on the Cytology requisition:
1. Patient's name, date of birth, hospital number, physician's name, hospital ward,
office or clinic, and date of specimen collection.
2. Specimen type/source.
3. Clinical diagnosis and pertinent clinical history.
4. Medications: hormones, chemotherapeutic drugs, etc.
5. Sex of patient.
For Surgery, Pequot Surgery, and inpatient units, cytology orders are entered in
McKesson or Meditech. (See Order Entry procedures).
Ascitic Fluid Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial
Breast Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc.
Bronchial Brushings, Cytology see Cytology, Brushings: Bronchial, Esophageal,
Gastric, Small Bowel, Colonic, Etc.
Bronchial Washings, Cytology see Cytology, Bronchial, Esophageal Washings and
Tracheal Aspirations
Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc.
Cytology, Body Fluids: Pleural, Ascitic and Pericardial
Synonyms: Pleural Fluid Cytology; Thoracentesis Cytology; Ascitic Fluid
Cytology; Paracentesis Cytology; Pericardial Fluid Cytology; Pericardiocentesis
Cytology
Test Includes: Cytologic examination and tissue cell block (when possible)
(Cytology)
Lab Performing Test: Cytology
Request Form: Cytology - include pertinent clinical information, e.g., previous
carcinoma, drugs, radiation therapy or history of alcohol abuse. Under source
include type of fluid.
Special Instructions: Deliver to Cytology lab immediately
Specimen Required: Fresh body fluid
Container Required: Vacutainer bottles obtained in central supply. Container
must be labeled in the presence of the patient and must include tha patient’s name,
date of birth, and specimen source.
Volume of Specimen: If 500 ml or less is obtained, send entire specimen to
Cytology. If more, send an aliquot of at least 500 ml.
Causes for Rejection: Improper labeling and improper container; i.e. plastic
evacuation bags, syringes.
Availability: Weekdays 8AM to 4:30PM,
Time Required Routinely for Processing: 24-48 hours
Additional Information: Specimen should be refrigerated if Cytology Lab is
closed
Reference Range: Negative
Cytology, Bronchial, Esophageal Washings and Tracheal Aspirations
Synonyms: Bronchial Washings Cytology; Esophageal Washings, Tracheal
Aspiration Cytology
General Use: To establish the presence of primary or metastatic neoplasm
Lab Performing Test: Cytology
Request form: Cytology - under source of specimen enter site of collection.
Include pertinent clinical information, e.g., previous carcinoma.
Special Instructions: Deliver to Cytology lab immediately
Specimen Required: Obtained by physician
Container Required: Available in central supply. Container must be labeled in
the presence of the patient and must include tha patient’s name, date of birth, and
specimen source.
Volume of Specimen: Not less than 1-2 cc
Causes for Rejection: Improper labeling
Availability: Weekdays, 8AM to 4:30PM
Time Required Routinely for Processing: 24-48 hours
Additional Information: Specimen should be refrigerated if Cytology lab is
closed
Reference Range: Negative
Cytology, Brushings: Bronchial, Esophageal, Gastric, Small Bowel, Colonic, Etc.
Synonyms: Bronchial Brushings, Cytology
General Use: To establish the presence of primary or metastatic neoplasm
Lab Performing Test: Cytology
Request Form: Cytology - under source of specimen specify the site brushed.
Include pertinent clinical data, e.g., admitting diagnosis, history of carcinoma.
Specimen Required: Brush lesion area, smear on slides (labeled with name, date
of birth and specimen type) and fix immediately in 95% alcohol, or cytology
spray fixative. Alternatively, immediately place the brush in Saccomanno’s
fixative or equivalent. Agitate the brush, which may be left in the fixative.
Container Required: Coplin jars and Saccomanno’s fixative are available in
Cytology lab. Container must be labeled in the presence of the patient and must
include tha patient’s name, date of birth, and specimen source.
Causes for Rejection: Improper labeling, improper fixation
Availability: Weekdays, 8AM-4:30PM
Time Required Routinely for Processing: 24-48 hours
Reference Range: Negative
Cytology, Cervical – Vaginal Smears
Synonyms: Papanicolaou Smear; Pap Smear ; Liquid-based cytology
General Use: To establish the presence of primary or metastatic neoplasm,
reactive processes or infectious disease; only offered for inpatients. Liquid-based
specimens may also be tested by molecular methods for GC/Chlamydia DNA and
HPV DNA. For HPV testing, specify whether to perform as a reflex for
ASCUS/AGUS, or regardless of pap result.
Lab Performing Test: All liquid based specimens will be sent to a reference lab
for processing and interpretation. In hospital conventional smears will be
screened and reviewed by Dr. Muscato, Dr. Green or Dr. Antic.
Request Form: Cytology - under source of specimen indicate: endocervical,
ectocervical or vaginal. Include pertinent clinical history, e.g., age, LMP, PMP,
surgery, exogenous hormones, history of carcinoma, radiation, chemotherapy.
Special Instructions: Slides frosted on one end should be labeled with the
patient's name, date of birth and source of specimen. Vaginal smears: We
recommend sampling the pool in the posterior vaginal vault. Cervical smears: A
360 degree scraping about cervical os using a wooden cervi-scraper is
recommended, also a cytobrush inserted into the endocervix and rotated 1/2 to 1
full turn is recommended. Place slides immediately in 95% alcohol or spray with
fixative. For liquid-based cytology (Cytyc ThinPrep or Roche SurePath), follow
manufacturer recommendations.
Specimen Required: "EXO and ENDO", "CX" and "ENDOCX" or "VAG" and
"CX"
Container Required: Pap kit organizers/slides, ThinPrep and SurePath supplies
are available in Specimen Processing.
Causes for Rejection: Improper labeling, of either slide or requisition.
Availability: Weekdays, 8AM to 4:30PM
Time Required Routinely for Processing: 24-72 hours, after receipt
Reference Range: Negative
Cytology, Nipple Discharge
Synonyms: Nipple Discharge Cytology
General Use: To establish the presence of primary or metastatic neoplasm
Lab Performing Test: Cytology
Request Form: Cytology - under source of specimen specify nipple discharge.
Include slide, pertinent clinical data, e.g., history of carcinoma.
Special Instructions: Label slides with name of patient, date of birth and source.
Place in 95% alcohol or cytology spray fixative immediately after taking slides.
Specimen Required: Nipple discharge
Container Required: Cytology fixative available in Cytology
Causes for Rejection: Improper labeling, improper fixation
Availability: Weekdays, 8AM to 4:30PM
Time Required Routinely for Processing: 24-48 hours
Reference Range: Negative
Cytology, Spinal and Cyst Fluid: Renal, Ovarian, Breast, Etc.
Synonyms: Spinal Fluid Cytology; Cyst Fluid Cytology: Renal Cyst Fluid
Cytology; Ovarian Cyst Fluid Cytology; Breast Cyst Fluid Cytology
General Use: To establish the presence of primary or metastatic neoplasm
Lab Performing Test: Cytology
Request Form: Cytology - under source of specimen specify specimen origin.
Include pertinent clinical data, e.g., admitting diagnosis, history of carcinoma.
Special Instructions: Deliver to Cytology lab immediately
Specimen Required: Fresh fluid
Container Required: If small amount of fluid, use sterile urine cups or urine
tube. Container must be labeled in the presence of the patient and must include
tha patient’s name, date of birth, and specimen source.
Volume of Specimen: Not less than 1-2 ml
Causes for Rejection: Improper labeling
Availability: Weekdays, 8AM to 4:30PM
Time Required Routinely for Processing: 24-48 hours
Additional Information: Specimen should be refrigerated if Cytology lab is
closed.
Reference Range: Negative
Cytology, Sputum
Synonyms: Sputum Series Cytology; Sputum Cytology
General Use: To establish the presence of primary or metastatic neoplasm
Request Form: Cytology - under source of specimen enter sputum. Include
admitting diagnosis and pertinent clinical history, e.g., history of carcinoma,
exposure to carcinogen
Specimen Required: Deep cough sputum, NOT SALIVA OR NASAL
ASPIRATES
Container Required: Sterile specimen container. Container must be labeled in
the presence of the patient and must include tha patient’s name, date of birth, and
specimen source.
Causes for Rejection: Improper labeling, saliva or nasal aspirates
Availability: Weekdays, 8AM to 4:30PM
Time Required Routinely for Processing: 24-48 hours
Additional Information: Specimen should be placed in refrigerator if Cytology
lab is closed.
Reference Range: Negative
Cytology, Urine
General Use: To establish the presence of primary or metastatic neoplasm
Lab Performing Test: Cytology
Request Form: Cytology - under source of specimen specify clean voided,
catheterized or bladder washing
Special Instructions: Urine specimen should be collected before patient has an
IVP. Specimen should be delivered to lab immediately. If from office, alcohol
may be added to the specimen. (50% ethanol, 1:1 by volume)
Container Required: Plastic urine container. Container must be labeled in the
presence of the patient and must include tha patient’s name, date of birth, and
specimen source.
Volume of Specimen: Not less than 10 cc, preferably 50 cc.
Causes for Rejection: Improper labeling, 24-hour collection
Availability: Weekdays, 8AM to 4:30PM
Time Required Routinely for Processing: 24-48 hours
Additional Information: Specimen should be refrigerated if Cytology lab is
closed.
Reference Range: Negative
Cytology: Viral Study: Cytomegalic Virus on Urine
Synonyms: Cytomegalic Inclusion Disease (CID); Cytomegalic Inclusion Bodies
General Use: To establish the presence of Cytomegalovirus infection
Lab Performing Test: Cytology
Request Form: Cytology - under source of specimen enter urine and specify for
Cytomegalic Inclusion Virus. Include pertinent clinical history, e.g.,
chemotherapy or immune-supression
Special Instructions: Deliver to Cytology lab immediately
Specimen Required: Fresh urine
Container Required: Sterile urine container. Container must be labeled in the
presence of the patient and must include tha patient’s name, date of birth, and
specimen source.
Volume of Specimen: Prefer 50 cc. Usually the greater the volume, the greater
the number of cells for evaluation
Causes for Rejection: Improper labeling
Availability: Weekdays 8AM-4:30PM
Time Required Routinely for Processing: 24-48 hours
Additional Information: Specimen should be refrigerated if Cytology lab is
closed.
Reference Range: No viral inclusion bodies seen
Cytomegalic Inclusion Bodies see Cytology: Viral Study: Cytomegalic Virus on
Urine
Cytomegalic Inclusion Disease (CID) see Cytology: Viral Study: Cytomegalic Virus
on Urine
Esophageal Washings, Tracheal Aspiration Cytology see Cytology, Bronchial,
Esophageal Washings and Tracheal Aspirations
Fine Needle Aspiration
General Use: To establish the presence of primary or metastatic neoplasm or
reactive process or infectious diseases
Lab Performing Test: Cytology Laboratory
Request Form: Cytology- under source of specimen indicate body site. Include
pertinent clinical history, i.e., date of birth, surgery, history of carcinoma,
radiation, chemotherapy, size of mass, etc.
Patient Preparation: Physician's responsibility
Special Instructions: Call X-Ray Department to schedule procedure. If
procedure is performed in office, slide must be properly labeled (patient name,
date of birth and specimen type), fixed with 95% alcohol or spray fixative and
submitted with a completed requisition. Alternatively, the needle aspirate can be
placed immediately in Saccomanno’s fixative or equivalent.
Container Required: Coplin Jar with 95% alcohol, or cytology spray fixative,
and Saccomanno’s fixative are available in Cytology. Container must be labeled
in the presence of the patient and must include tha patient’s name, date of birth,
and specimen source.
Causes for Rejection: Improper labeling of specimen and incorrect specimen
containers (no syringes with needles)
Availability: Weekdays, 8AM to 4:30PM; Stat procedure available during
weekday hours
Time Required Routinely for Processing: 24-48 hours
Additional Information: Cytology staff available for assistance. For questions
please call ext. 5121
Reference Range: Negative
Nipple Discharge Cytology, see Cytology, Nipple Discharge
Ovarian Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc.
Pap Smear see Cytology, Cervical - Vaginal Smears
Papanicolaou Smear see Cytology, Cervical - Vaginal Smears
Paracentesis Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial
Pericardial Fluid Cytology see Cytology, Body Fluids: Pleural, Ascitic and
Pericardial
Pericardiocentesis Cytology see Cytology, Body Fluids: Pleural, Ascitic and
Pericardial
Pleural Fluid Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial
Renal Cyst Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian,
Breast, Etc.
Spinal Fluid Cytology see Cytology, Spinal and Cyst Fluid: Renal, Ovarian, Breast,
Etc.
Sputum Cytology see Cytology, Sputum
Sputum Series Cytology see Cytology, Sputum
Thoracentesis Cytology see Cytology, Body Fluids: Pleural, Ascitic and Pericardial
Tzanck Preparation
Synonyms: Tzanck smear, smear for cutaneous herpes
General Use: To establish the presence of Herpes Simplex Virus
Lab Performing Test: Cytology
Request Form: Cytology - under source of specimen indicate site. Include
pertinent clinical information
Specimen Required: Brush, scrape lesions, smear on appropriately labeled slides
(patient name, date of birth and specimen type) and fix immediately in 95%
Alcohol or cytology spray fixative
Container Required: Available from Cytology lab
Causes for Rejection: Improper labeling or fixation
Availability: Weekdays 8AM-4:30PM
Time Required Routinely for Processing: 24-48 hours, Stat on request
Reference Range: Negative
HEMATOLOGY
The Hematology section of the Laboratory is directed by Victoria G. Reyes-D'Arcy/
Asim Ejaz, M.D., D.A.B. Pathologist, and Pedro Ugarelli, M.S., B.S., M.T., Hematology
Manager.
This section offers a wide range of services including coagulation, urinalysis,
hematology, and body fluid analyses. For procedures not listed please consult with the
Hematology Manager(Ext. 4137) or Pathologist.
All specimens must be properly labeled with patient's full name, identification number,
time and date of collection, and the test requested.
Babesia Smear
General Use: Rapid Screening for Babesia
Test Mnemonic: BABSMR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instruction:
Specimen Required: Whole Blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 4.0 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Daily – not STAT
Time Required Routinely for Processing: 2 hours
Reference Range: Negative
Body Fluid Analysis
Synonyms: Body fluid cell count.
General Use: Evaluation of cellularity and cell types contained in various body
fluids for determining infection, malignancy, inflammatory reaction.
Test Mnemonic: FCT
Profile Includes: Body fluids (other than synovial fluid) includes WBC and RBC
enumeration and review of cell morphology by a pathologist
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Responsibility of Physician
Special Instructions: Anatomic origin of fluid required, i.e., chest, abdomen,
etc.; deliver to the lab immediately upon collection
Specimen Required: Thoracentesis or paracentesis fluids, miscellaneous body
fluids.
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml
Minimum Volume of Specimen: 4.0 ml
Causes for Rejection: Clotted specimen, insufficient specimen volume,
mislabeled or unlabeled specimen.
Availability: Cell Count - daily first and second shifts; Pathologist review of cell
morphology Monday through Friday
Time Required for Processing: Body fluid analysis - up to 4 hours (pathology
review only on weekdays)
Reference Range: See ranges on printed report.
Body Fluid Specific Gravity
Synonyms: Fluid specific gravity
General Use: Determining if a fluid is an exudate or transudate in conjunction
with other testing
Test Mnemonic: FSPGR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Responsibility of physician
Special Instructions: Site of collection required, i.e., chest, knee, etc.
Specimen Required: Body fluid
Container Required: Urine container
Volume of Specimen: 5.0 ml of fluid
Minimum Volume of Specimen: 1.0 ml
Causes for Rejection: Clotted specimen, inadequate specimen volume,
mislabeled or unlabeled specimen
Availability: 24/7
Time Required Routinely for Processing: up to 2 hours
Reference Range: Physician interpretation.
Bone Marrow
General Use: Bone marrow aspirate/biopsy/clot section morphology, including
cellularity, myeloid/erythroid ratio, and iron stores
Test Mnemonic: Cannot be ordered in Meditech
Profile Includes: H + E stain, Wright's stain, and iron stain
Lab Performing Test: Hematology, Histology, and Pathology
Request Form: Bone Marrow Request form (filled out in entirely by physician)
Patient Preparation: Performed by physician
Special Instructions: Call Hematology for bone marrow tray
Specimen Required: Bone marrow biopsy and bone marrow aspirate
Container Required: Lavender top tube (EDTA) for bone marrow aspirate;
formalin for bone marrow biopsy
Volume of Specimen: 1.0 ml of bone marrow aspirate (lavender top tube)
Minimum Volume of Specimen: 1.0 ml
Causes for Rejection: Mislabeled or unlabeled specimen.
Availability: Mon. - Fri.; Not STAT, Not available on weekends or holidays.
Bone Marrow samples must arrive in Hematology Lab before 2 PM .
Time Required for Processing: Specimen prepared same day; Pathology report:
48-72 hours for biopsy
Reference Range: Results interpreted by Pathology.
CBC see Complete Blood Count
Chronic Leukemia/Lymphoma Immunophenotyping
Synonyms: Leukemia/Lymphoma Panel, CLLP
General Use: Immunophenotypic markers for hematopoietic cells, leukemia,
lymphoproliferative disorders.
Test Mnemonic: CLLP
Lab Performing Test: Specimen Processing, sent to Reference Lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Clinical history required
Specimen Required: Whole blood
Container Required: 1 Green top tube (sodium heparin) or 1 lavender (EDTA)
Volume of Specimen: 8.0 ml of blood
Minimum Volume of Specimen: 8.0 ml of blood
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Daily, Mon.-Fri.
Time Required Routinely for Processing: 1-2 days
Reference Range: Interpretation made by pathologist
Cerebrospinal Fluid Analysis
Synonyms: CSF cell count; spinal fluid cell count.
General Use: Evaluation of CSF for cellularity and cell typing in determining
infection, intracranial bleed, malignancy, and other disease states.
Test Mnemonic: CCT
Profile Includes: WBC and RBC enumeration and WBC differential count
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Responsibility of physician
Special Instructions: Specific tests to be performed on each collection tube must
be indicated; deliver specimens to lab immediately upon collection.
Specimen Required: CSF
Container Required: Sterile CSF collection tube(s)
Volume of Specimen: 2.0 ml
Causes for Rejection: Clotted specimen, inadequate specimen volume,
mislabeled or unlabeled specimen.
Availability: 24 hours daily, always performed STAT
Time Required for Processing: 2 hours.
Reference Range: See printed report.
Complete Blood Count
Synonyms: CBC; Hemogram
General Use: Evaluation of peripheral blood parameters
Test Mnemonic: CBC
Profile Includes: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLCT and
6-part automated differential.
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: EDTA Lavender top tube or Microtainer.
Volume of Specimen: 4.0 ml of blood (lavender top tube) or 0.75 ml of blood
(lavender Microtainer)
Minimum Volume of Specimen: 4.0 ml (lavender top tube) or 0.5ml
(Microtainer)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours daily, routine and STAT
Time Required for Processing: Stat 30 minutes; routine same day.
Reference Range: See accompanying ranges by age and gender in printed report
Complete Blood Count with Manual Differential
Synonyms: CBC and Manual Diff; Hemogram and Manual Diff.
General Use: Evaluation of peripheral blood parameters
Test Mnemonic: CBC
Profile Includes: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLCT and
manual differential count and review of morphologic characteristics of blood cells
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: EDTA Lavender top tube or Microtainer.
Volume of Specimen: 4.0 ml of blood (lavender top tube) or 0.75 ml of blood
(lavender Microtainer)
Minimum Volume of Specimen: 4.0 ml (lavender top tube) or 0.5ml
(Microtainer)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours daily, routine and STAT
Time Required for Processing: Stat 30 minutes; routine same day.
Reference Range: See accompanying ranges by age and gender in printed report
CSFCT see Cerebrospinal Fluid Analysis.
Ehrlichia Smear
General Use: Rapid screening for Ehrlichia
Test Mnemonic: EHRSMR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instruction: Test will not be performed without a prior CBC. If the WBC
is greater than 4.0 and/or platelet count is greater than 120,000/mL the test will
not be performed.
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 4.0 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Daily - not STAT
Time Required Routinely for Processing: 4 hours
Reference Range: Negative
Eosinophil Smear
Synonyms: Nasal smear for eosinophils; Sputum smear for eosinophils
General Use: Investigation of allergic disorders
Test Mnemonic: EOSM
Lab Performing Test: Hematology send out
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Smear or swab of sputum, 2 slides of nasal secretion
Causes for Rejection: Insufficient specimen, mislabeled or unlabeled specimen.
Availability: Daily - Not STAT
Time Required Routinely for Processing: Same day except for weekends
Reference Range: None seen
Erythrocyte Sedimentation Rate see Sedimentation Rate
ESR see Sedimentation Rate
FCEL see Body Fluid Analysis
Fetal Hemoglobin Stain
Synonyms: Kleihauer-Betke Fetal Hgb Stain
General Use: Qualitative evaluation of Hgb F, if present; to determine possible
hemorrhage in the newborn; to assess the magnitude of fetal - maternal
hemorrhage.
Test Mnemonic: FHGBS
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 4.0 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Daily, routine and stat until 10PM; Not done from 10PM to 7AM
Time Required Routinely for Processing: Same day
Reference Range: 0.000 (See Table II)
FHCT see Hematocrit - Body Fluid
Fluid Crystals
General Use: Aid in the diagnosis of gout or pseudogout.
Test Mnemonic: BFCRYS
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Responsibility of physician
Special Instructions: Site of fluid extraction should be noted, ie., right knee, left
elbow, etc.
Specimen Required: Body fluid specimens. Specify body site.
Container Required: Lavender top tube (EDTA) or green top tube (sodium
heparin)
Volume of Specimen: 2.0 ml (lavender top tube)
Minimum Volume of Specimen: 2.0 ml
Causes for Rejection: Insufficient specimen volume, mislabeled or unlabeled
specimen
Availability: Daily first and second shift; not stat
Time Required Routinely for Processing: Up to 4 hours
Reference Range: None seen
Fluid Hematocrit
Synonyms: Body fluid hematocrit
General Use: Physician interpretation
Test Mnemonic: FHCT
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Responsibility of physician
Specimen Required: Body fluid
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 1.0 ml (lavender top tube)
Minimum Volume of Specimen: 1.0 ml
Causes for Rejection: Clotted specimen, insufficient specimen volume,
mislabeled or unlabeled specimen.
Availability: 24/7
Time Required for Processing: 1- 2 hours
Hemogram see Complete Blood Count
Hematocrit - Peripheral Blood
General Use: Evaluation of peripheral blood packed cell volume
Test Mnemonic: HCT
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Lab Performing Test: Hematology
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA); lavender top Microtainer for
infants.
Volume of Specimen: 4.0 ml of blood (lavender top tube) or 0.75 ml blood
(lavender top Microtainer)
Minimum Volume of Specimen: 4.0 ml (lavender top tube) or 0.50 ml (lavender
top Microtainer)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours, routine and STAT
Time Required for Processing: Stat -30 minutes; routine-same day.
Reference Range: See printed report for reference ranges by age and gender.
Hemoglobin-Peripheral Blood
General Use: Evaluation of peripheral blood hemoglobin level
Test Mnemonic: HGB
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood (EDTA)
Container Required: Lavender top tube (EDTA); lavender top Microtainer for
infants
Volume of Specimen: 5.0 ml of blood (lavender top tube) or 0.75 ml blood
(lavender to Microtainer)
Minimum Volume of Specimen: 2.0 ml (lavender top tube) or 0.50 ml blood
(lavender top Microtainer)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: 24 hours, routine and STAT.
Time Required Routinely for Processing: Stat:30 minutes; Routine: same day.
Reference Range: See printed report for reference ranges by age and gender.
Hemogram see Complete Blood Count
Iron Stain, Bone Marrow - see Bone Marrow
Kleihauer-Betke Fetal Hgb Stain - see Fetal Hemoglobin Stain
Malaria Smear
General Use: Rapid Screening for Malaria
Test Mnemonic: MALSMR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instruction:
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 4.0 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Daily – not STAT
Time Required Routinely for Processing: 4 hours
Reference Range: Negative.
Manual Differential see Differential White Blood Cell Count
Nasal Smear for Eosinophils see Eosinophil Smear
Platelet Count
General Use: Enumeration of platelets
Test Mnemonic: PLCT
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Use 21 or 22 gauge needle to obtain specimen; Do not use
butterfly needle. If specimen is drawn from an in-dwelling catheter, line must be
flushed with saline and the first 10 ml of blood collected must be discarded.
Specimen Required: Whole blood.
Container Required: Lavender top tube (EDTA).
Volume of Specimen: 4.0 ml of whole blood (Lavender top tube).
Minimum Volume of Specimen: 2.0 ml
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: 24 hours routine and STAT.
Time Required Routinely for Processing: Routine same day; Stat: 30 minutes.
Reference Range: See printed report for reference ranges by age and gender.
Retic Count see Reticulocyte Count
Reticulocyte Count
Synonyms: Retic Count
General Use: Enumeration of reticulocytes
Test Mnemonic: RET
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA).
Specimen Required: Whole blood.
Container Required: EDTA Lavender top tube or Microtainer.
Volume of Specimen: 4.0 ml of blood (lavender top tube) or 0.75 ml blood
(lavender top Microtainer).
Minimum Volume of Specimen: 2.0 ml (lavender top tube) or 0.50 ml (lavender
top Microtainer)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen.
Availability: Routine and STAT.
Time Required Routinely for Processing: Stat:30 minutes; Routine: 2hours.
Reference Range: See printed report for reference ranges by age and gender.
RTC see Reticulocyte Count
Sedimentation Rate
Synonyms: ESR, Sed Rate
General Use: To assess non-specific activity of disease processes
Test Mnemonic: ESR
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Draw an extra full lavender top tube if a CBC is not
ordered as well
Specimen Required: Whole blood
Container Required: Black top Vacu-Tech tube (special collection tube)
Volume of Specimen: 1.2 ml of blood
Minimum Volume of Specimen: 1.2 ml of blood
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:1 hour; Routine: same day
Reference Range: See printed report for reference ranges by age and gender.
Sed Rate see Sedimentation Rate
Sickle see Sickle Cell Preparation
Sickle Cell see Sickle Cell Preparation
Sickle Cell Preparation
Synonyms: Sickle Cell; Sickle
General Use: Detection of sickling hemoglobins
Test Mnemonic: SICKL
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Test cannot be performed on infants
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA)
Volume of Specimen: 4.0 ml of blood (lavender top tube)
Minimum Volume of Specimen: 2.0 ml (lavender top tube)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: Tuesday & Friday
Reference Range: Negative
Spinal Fluid Cell Count see Cerebrospinal Fluid Analysis
Sputum for Eosinophils see Eosinophil Smear
Synovial Fluid Analysis
Synonyms: Synovial fluid cell count
General Use: Evaluation of synovial fluid for inflammatory reaction,
hemorrhage, or infection.
Test Mnemonic: FCT
Profile Includes: WBC and RBC enumeration and WBC differential count.
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA).
Patient Preparation: Responsibility of physician.
Special Instructions: Indicate site of fluid extraction, ie., left knee, right elbow,
etc.
Specimen Required: Synovial fluid.
Container Required: EDTA Lavender top tube.
Volume of Specimen: 4.0 ml of synovial fluid
Minimum Volume of Specimen: 2.0 ml
Causes for Rejection: Clotted specimen, insufficient specimen volume,
mislabeled or unlabeled specimen
Availability: 24 hours, routine and stat; differential may be left for review on the
following morning
Time Required Routinely for Processing: 2 hours
Reference Range: See printed report for reference ranges by age and gender.
WBC see White Blood Cell Count
White Blood Cell Count
Synonyms: WBC
General Use: To determine the total leukocyte count of a peripheral blood
specimen
Test Mnemonic: WBC
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Whole blood
Container Required: Lavender top tube (EDTA) or lavender top Microtainer for
infants
Volume of Specimen: 4.0 ml of blood (lavender top tube) or 0.75 ml (lavender
top Microtainer)
Minimum Volume of Specimen: 2.0 ml (lavender top tube) or 0.50 ml (lavender
top Microtainer)
Causes for Rejection: Clotted or hemolyzed specimen, incorrect specimen
volume, mislabeled or unlabeled specimen
Availability: 24 hours daily, routine and STAT
Time Required Routinely for Processing: Stat: 30 minutes; routine: same day
Reference Range: See printed report for reference ranges by age and gender
HOME BLOOD DRAWING
As a convenience to our homebound patients, we offer a home blood drawing
service. We prefer at least one day’s notice prior to drawing blood.
The attending physician may request home blood-drawing services by faxing a
L&M HOME DRAWING REQUEST FORM. A blank L&M HOME DRAWING
REQUEST FORM may be requested by calling the main laboratory (860-4445101 or 860-444-5102).
MICROBIOLOGY
The Microbiology section is directed by Elise Krejci, M.D. and Managed by Peter
Speciale, MHS, PHD, M(ASCP)sm. The Microbiology Manager can be reached at 4420711 x4625.
MICROBIOLOGY COVERAGE
The following essential services in Microbiology are provided by laboratory personnel on
a 24-hour basis, seven days a week. The regular microbiology staff will be on duty
Monday through Sunday 7AM - 11 PM. At all other times, requests for service are to be
directed to the main laboratory. The services available on a 24-hour basis include:
1.
2.
3.
4.
5.
6.
7.
Plating of cultures (CSF as a STAT, others by special request).
CSF gram stain (others by special request).
Blood culture collection (service provided by the Laboratory at all times).
FFN (Fetal Fibronectin) testing
Rapid Beta Strep Screen
FLU A/B H1N1 by PCR
Chlamydia/GC PCR
MICROBIOLOGY REQUISITIONING
A. For Meditech, the test mnemonic is given. Microbiology procedures must be
ordered in the category MIC
B. The manual system (back up for Meditech) must be observed in all cases using
the Universal Requisition:
1.
2.
3.
4.
5.
Use a separate form for each specimen submitted.
Indicate the date and time that the specimen is collected.
Indicate the clinical diagnosis or impression relevant to the culture if pertinent.
Indicate the specimen source.
Patient name, date of birth, location, and physician requesting the test must be
filled in accurately.
6. All specimen containers must be completely labeled with the patient full name,
DOB, date and time of collection and specimen source.
7. All requisitions must be filled out in ink. Patient information should be stamped
on the requisition if possible
COLLECTION AND TRANSPORT OF MICROBIOLOGY SPECIMENS
GENERAL GUIDELINES FOR PROPER SPECIMEN COLLECTION
1. Collect specimens before administering antimicrobial agents when possible.
2. Collect specimens with as little contamination from indigenous organisms as
possible to ensure that the sample will be representative of the infected site.
3. Utilize appropriate collection devices. Use sterile equipment and aseptic
technique to collect specimens in order to prevent the introduction of
microorganisms during invasive procedures.
4. Clearly label the specimen container with the patient's full name and date of birth,
date and time of collection, and the specimen source.
5. Collect an adequate amount of specimen. Inadequate amounts of specimen may
yield false-negative results.
6. Identify the specimen source and/or specific site correctly so that the proper
culture media will be selected during processing in the laboratory.
7. Collect specimens in sturdy, sterile, screw-cap, leakproof containers with lids that
do not create an aerosol when opened. Be sure all lids are screwed on tightly!
8. Use appropriate transport systems for special test procedures (example: Genprobe transport tubes for GC and Chlamydia DNA Probe, viral swabs and viral
transport media for viral cultures, anaerobic transport systems for anaerobic
cultures etc.). Contact the microbiology laboratory before collecting specimens if
there is any question
9. Specimens for testing for specific pathogens by molecular amplification
methodology e.g. “amplified DNA” or “PCR” should not be opened again, once
placed in their container. Any manipulation of the specimen must be performed in
the lab biosafety cabinets, using sterile technique. This is to ensure that no other
organism DNA, including human enters the specimen container.
10. All specimens must be accompanied by a Meditech requisition (or a Universal
requisition as Meditech backup). A separate requisition must be submitted for
each test requested. The requisition must include:
a.
b.
c.
d.
e.
f.
g.
h.
the patient's full name
date of birth
the patient's location
the ordering physician
the source and/or specific site of the specimen
the type of examination requested
the diagnosis (if available)
the date and time of collection
GENERAL GUIDELINES FOR PROPER SPECIMEN TRANSPORT
All specimens should be transported to the Microbiology Laboratory promptly (within 1
to 2 hours) to ensure the survival and isolation of fastidious organisms, and to prevent
overgrowth by more hardy bacteria in order to provide a more accurate diagnosis of the
infectious disease process. Certain specimens may have different time restrictions and
these are noted in the specific listings.
Routine cultures must be received in the Microbiology Laboratory by 10:00 PM to allow
time for processing.
ALTERNATIVES TO PROMPT DELIVERY
1. Refrigerate most specimens at 2 to 8 degrees C. The following are exceptions:
a. If blood cultures are not delivered to the laboratory immediately upon
collection they should remain at room temperature until delivery.
b. Specimens that may harbor temperature-sensitive organisms such as
Neisseria species should be left at room temperature. Do not refrigerate
genital specimens!
c. For anaerobic specimens, the use of an anaerobic transport system is
mandatory. A regular culturette will not ensure the viability of fastidious
anaerobes. Do not refrigerate anaerobic specimens!
d. Stool specimens should be received within 2 hours of their collection;
some pathogens may begin to die off after the first two hours. Specimens
should be refrigerated if there will be a delay in transport to the laboratory.
e. For swab cultures, a Culturette type collection/transport system should be
used - be sure to crush the ampule of holding media if the system has one.
Dry swabs are not acceptable for culture.
REJECTION OF SPECIMENS
Specimens will be rejected for the following reasons:
1. Mislabeled or unlabeled specimens and incomplete labeled specimens.
2. Specimens with insufficient quantity.
3. Specimens which have leaked from their container or are received with the
container open.
4. Improper collection or transport devices.
5. Improperly stored specimens.
6. Specimens received after prolonged delay from time of collection. In general any
specimen which is received greater than 24 hours after collection will be rejected.
7. Specimens other than stool which are received for culture in unsterile containers.
8. Sputum received as obvious saliva as determined by gross examination or gram
stain.
9. Material received for anaerobic culture in a non-anaerobic collection tube. The
culturette system will not ensure the viability of all anaerobes and is not
acceptable for an anaerobic culture. Sputum, urine or cervical specimens received
for anaerobic culture will be rejected since they are inappropriate sources for
anaerobic culture.
10. Duplicate specimens will be rejected unless there is some indication on the
requisition of anatomic or timing differences. Physician orders for cultures must
be specific.
The appropriate nursing station, clinic or physician will be notified by phone prior to the
disposal of an unsatisfactory specimen and a repeat specimen will be requested. If the
physician, for whatever reason, insists that the results of an improperly selected,
collected, or transported specimen be reported, the Microbiology Laboratory will include
in the report a statement explaining the potential compromised nature of the results.
SPECIMEN COLLECTION
A. Urine Cultures
1. Specimens for routine urine culture must be refrigerated after collection.
They will be screened for fast growing aerobic pathogenic bacteria and
Candida species. The following types of organisms are examples of those not
isolated by routine urine culture:
Neisseria gonorrhoeae, Leptospira and anaerobic bacteria. These must be
requested separately - call Microbiology for specific instructions.
2. Suprapubic puncture or bladder washings are to be collected by the physician
so as to avoid contamination with adjacent structures and surfaces. Cultures
with any colonies will be identified. Antimicrobial susceptibility tests will be
performed if appropriate.
3. Straight Catheterized urine specimens are to be collected by the physician or
catheter care team so as to avoid contamination with adjacent structures and
surfaces. Pure growth or cultures with 2 or less organisms will be identified
and antimicrobial susceptibilities will be performed if appropriate. Cultures
with 3 or more organisms will receive rough identification.
4. Mid-stream urine specimens/Foley catheter urine specimens
a. Midstream specimens are to be collected by the patient after thorough
cleansing of skin with soap and water or wipe provided. Only the
middle portion of the voided specimen is to be collected.
b. Criteria for organism identification. Organisms occurring in numbers
less than 10,000 per ml will not be identified nor will susceptibility
tests be performed unless specific instructions to the contrary are noted
on the requisition. Organisms occurring in numbers greater than
10,000 per ml will be identified and susceptibility tests will be
performed if the numbers of different types of organisms are not
suggestive of contamination with mixed enteric and/or skin flora. If
three or more different organisms are isolated from an individual
specimen, the report will state that these organisms are suggestive of
contamination with mixed enteric and/or skin flora and a repeat
specimen will be requested.
5. Urine specimens submitted to the laboratory that are not identified by
collection method will be worked up according to the mid-stream/foley
catheter identification criteria.
B. Respiratory Cultures
The following collection sites are considered respiratory specimens: nose,
nasopharyngeal swabs, throat, sputum, bronchial and tracheal aspirations and mouth. All
specimens from these sites will be screened for fast-growing aerobic pathogenic
organisms. The following organisms are examples of those not isolated by routine
culture: Neisseria gonorrhoeae, Corynebacterium diptheriae, and Bordetella sp. For
information on culture of non-routine organisms see the culture listing for the specific
organism.
1. Throat specimens
a. collection - throat swabs should be collected so as to avoid contamination
by mouth and tongue.
b. Beta Strep Screens for Group A strep will be screened for only that
organism.
c. Throat specimens for Neisseria gonorrhoeae will be screened only for that
pathogen and must be specifically requested.
2. Nose or nasopharyngeal specimens
a. Collection - to be collected so as to avoid contamination by adjacent
structures.
3. Sputum specimens
a. Collection - to be collected by instructing the patient to remove dentures, rinse
mouth, gargle with water and cough deeply, expectorating into appropriate
collection container.
b. All specimens labeled "sputum" but actually consisting of saliva or upper
respiratory secretions will be rejected. Rejection or acceptance will be based
on one or both of the following criteria:
1. Gross examination for mucopurulent material.
2. Microscopic examination for mucopurulent material,
presence/absence of squamous epithelial cells and presence/absence
WBC’s.
c. Specimens obtained by transtracheal aspiration, bronchoscopy, or sputum
induction procedure will not be subjected to this screening procedure. Use of
these collection techniques must be clearly stated on the requisition.
Specimens from transtracheal aspiration can be screened for anaerobic
pathogens if requested.
C. Blood Cultures
1. Collection of specimens - Blood cultures are usually collected by the Laboratory;
however, should the physician choose to collect the specimen he/she should
contact the laboratory for proper equipment and procedures. The blood culture
bottles must be labeled with the patient's name, date of birth, and the date and
time of collection. The blood culture collection procedure can also be found in
Meditech in the Laboratory section of patient care services.
2. All routine blood cultures are screened for aerobic and anaerobic pathogens by
automated Bac-T-Alert methodology. All organisms isolated from blood cultures
will be identified, susceptibility tests performed if appropriate. Organisms which
are considered likely to be skin contaminants are coagulase negative Staph
species , Bacillus sp, Diphtheroid sp, Propionibacterium and Alpha Strep.
D. Miscellaneous Cultures
1. Specimens from normally sterile sites such as CSF, bone marrow, surgical
specimens, joint fluids, etc., will be cultured for aerobic pathogenic
organisms. If an anaerobic organism is suspected two swab specimens or
adequate volume should be submitted for both aerobic and anaerobic culture
and transported in the appropriate container. See the specific Anaerobic
Culture listing for further details.
2. Bone marrow, eye swabs, joint fluids and spinal fluids will be routinely
screened for fast-growing aerobic pathogenic organisms in addition to the
fastidious species of Haemophilus and Neisseria .
3. Other body fluids and surgical specimens (including tissue) will be cultured
for fast-growing aerobic pathogens.
4. Genital specimens are routinely cultured to isolate Neisseria gonorrhoeae (DO
NOT REFRIGERATE) , Listeria and to determine the presence of Beta
Hemolytic strep, and Staphylococcus aureus. If yeast is suspected a Candida
culture should be ordered. If other pathogens are suspected, it should be noted
on the order or specifically requested.
5. Specimens from wounds, abscesses, incisions, and pus will be screened for
fast-growing aerobic organisms. If an anaerobe is suspected, a specimen must
be properly submitted to the laboratory. See the specific Anaerobic Culture
listing for further details.
E. Stool Cultures
1. Ideally specimens for routine stool culture should not be older than 2 hours (see
stool collection/transport guide). They must be properly collected in feces
containers and covered. Homemade containers or containers lined with plastic,
tissue paper, newspaper, etc. are unacceptable. Stool specimens should not be
submitted in a diaper.
2. Rectal swabs for routine culture should not be older than 2 hours. (see stool
collection/transport guide) The medium compartment on the swab must be
broken.
3. Do not freeze stool specimens.
4. Stools will be routinely screened for Salmonella, Shigella, Campylobacter and E.
coli 0157. If other pathogens are suspected, the appropriate test should be ordered
so the proper media will be inoculated.
5. For routine culture no more than one specimen should be collected within a 24hour period. One specimen a day for three days may be submitted for optimum
diagnosis.
F. Anaerobic Culture Specimens
1. Anaerobic Specimens must be collected in a special anaerobic
collection/transport tube.
2. Criteria for Rejection - culture sites unacceptable for anaerobic culture:
a.
b.
c.
d.
e.
f.
g.
Throat or nasopharyngeal swab
Sputum or bronchoscopic specimens.
Feces or rectal swabs.
Mid-void or catheterized urine specimens.
Vaginal or cervical swabs.
Material from superficial wounds.
Material from abdominal wounds obviously contaminated with feces
(e.g. open fistula).
3. Handling problems
a. Liquid and swab specimens must be transported in anaerobic transport
media/container.
b. All specimens should be received in the laboratory within 2 hours of
collection. DO NOT REFRIGERATE!
4. Unacceptable Specimens
a. Any specimens from inappropriate culture sites, improperly handled
specimens, or those delayed in transport, are unacceptable for
anaerobic culture.
G. Viral Cultures
Viral cultures are referred to a reference lab and must be placed in appropriate transport
medium.
IMPORTANT GENERAL REQUIREMENTS FOR VIRAL CULTURES
Early Collection of Specimens - Material for virus isolation must be collected within a
few days following onset of illness. By 7 days after onset it is generally too late to obtain
satisfactory specimens for virologic diagnosis.
Identification of Specimens - Clearly label each specimen with patient name, DOB, and
date of collection and source. Indicate viral agent suspected and ordered test.
Viral transport media is available in the microbiology laboratory.
COLLECTIONS OF SPECIMENS FOR VIRAL ISOLATION
When to Collect Specimens - Specimens should be collected as soon as possible after
onset.
How to Collect Specimens - The collection of the following specimens can best be
accomplished by:
Throat swab - Swab posterior wall of pharynx.
Rectal swab - Insert swab 4-6 cm into rectum and rub against wall of mucosa until
fecal material is seen to adhere to the swab.
Vesicular lesion - Open mature vesicle, collect fluid on swab. Ulcerative lesions
can be swabbed directly.
Nasal swab - Insert dry swab into nasal passage and allow it to absorb secretions.
Conjunctiva - Remove exudate before swabbing conjunctiva.
Certain specimens require special handling:
Spinal Fluid - Collect 3-5 ml in a sterile screw cap tube. Preferably, The 2nd or 3rd
tube collected should be submitted for microbiological analyses.
Urine - Collect 10-15 ml of urine in a sterile screw cap tube. (First morning
specimens are preferred.) Note: specimens for isolation of cytomegalovirus (CMV) must
be refrigerated and transported immediately to the laboratory on wet ice. Monday to
Thurs. 7 AM – 10:00pm. Friday specimens must be rec’d by 12 noon..Specimens
received Friday pm, Sat. Sun. and holidays will be processed on the next routine day.
Autopsy specimens - Small tissue specimens roughly 2 cm square should be
collected from areas of probable pathology with a separate sterile instrument for each
specimen. Tissue specimens should be submitted in a sterile screw cap tube without
preservatives. Tissue specimens that cannot be submitted directly should be frozen at -70
degrees C and submitted on dry ice.
H. Parasitology Specimens
Specimens for ova and parasites will be sent to a reference laboratory, Monday through
Friday from 7:00 AM to 11:am. Any specimens received Friday after 11:00 AM or on
weekends or holidays will be placed in a fixative and will be submitted on the next
routine day. NOTE: If Cryptosporidium is suspected, Cryptosporidium antigen detection
is the preferred test as an Ova and Parasite exam in most cases does not detect this
parasite.
Criteria for Collection of Stool Specimens:
Stool Collection/Transport Guide
Specimens should be collected in CLEAN, DRY wide-mouth containers with an
appropriate lid forming a tight leak-proof fit. Specimens that are contaminated with urine
or water are cause for rejection. Specimens should be transported to the laboratory as
soon after collection as possible for optimal organism recovery. The following will serve
as a guide when this is not possible. Approximately 5gm or 5ml of specimen per test
(additional tests require additional volumes) is adequate unless otherwise specified.
TEST:
Stool Culture
Whole stool/
Rectal swabs
TRANSPORT TIME:
2 hours at room temp.
Within 24 hours/4 C
(refrigerated)
INTERVAL:
24 hours
Rotavirus
72 hours/4 C
(pea sized portion)
(Refrigerated)
(swab unacceptable)
Days 3 – 5 post onset
of symptoms
C. difficile toxin
24 hours minimum
24 hours/4 C
O&P 15gm/15ml
<30 minutes room temp
24hrs/4 C
Cryptosporidium Antigen
24 hours/4C
24 – 48 hours
*See below
2X recommended if 1st
Negative and symptoms persist
Cyclospora
24hours/ 4C
same as O&P
Giardia Antigen
24hrs/4 C
Occult Blood
within 72 hrs
Pinworm Exam
no time limit but must be collected on pinworm paddle device.
2X recommended if 1st
Negative and symptoms persist
each bowel movement
Recommendation for O&P evaluation
A Minimum of three specimens collected every day or every other day but not within the
same day should be collected. Six specimens are recommended for the diagnosis of
amebiasis. Specimens should be collected during a time frame of no more than ten days.
If a series of six specimens is ordered, then they should be submitted within a 14-day
period.
Cause for rejection:
Specimens will be rejected by the lab for determinations; if taken after a barium enema,
taken after the administration of castor oil, taken after administration of laxatives,
suppositories, or contaminated with urine.
I. Additional Information:
Special Susceptibility Testing:
Requests for special antimicrobial susceptibility testing or for retaining a particular
bacterial isolate for additional testing should be made as soon as possible after
submission of the culture and before the final report is issued. Most organisms are not
routinely held after finalization.
(Exception: Blood Culture isolates are routinely retained for 30 days.)
Tests other than those listed are often available through a reference laboratory.
Please contact the Microbiology Department at ext. 5192 for information.
AFB Blood Culture see Culture, Mycobacteria, Blood
AFB Smear and Culture, MAV Culture, TB Culture see Culture, Mycobacteria
Beta Strep Group A (Rapid Test)
Synonyms: 10 Minute Beta Strep Screen
General Use: Rule out Beta Strep Group A
Test Mnemonic: BSSC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Throat swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container
Availability: 24 hours daily
Time Required Routinely for Processing: Negative & positive results will
normally be reported 30 minutes after arrival in the lab
Additional Information: Requisition must clearly state "For 10 minute Beta
Strep Screen" NOTE: This test is considered presumptive when negative and a 24
hour Beta Strep Screen is recommended for confirmation
Reference Range: Negative by Direct (Rapid) Beta Strep screen
Beta Strep Screen (24 hour) see Culture, Throat, for Beta Strep Group A
Blood Culture for MAV see Culture, Mycobacteria, Blood
Blood Culture, Fungus see Culture, Blood
Blood Culture, Routine see Culture, Blood
C. Difficile Toxin
Synonyms: Clostridium Difficile Toxin, Stool for C.diff
General Use: To rule out C. difficile infection
Test Mnemonic: CDIFF
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool
Container Required: Sterile cup, stool cup
Volume of Specimen: 1-2 ml
Collection: Random, no more than one specimen should be submitted.
Availability: Performed 24/7.
Time Required Routinely for Processing: 1-2 hours
Additional information: Test detects toxigenic Cdiff and the epidemic strain 027
.
Reference Range: Negative
Candida Antigen
General Use: To determine diagnosis & prognosis of Candida infection
Test Mnemonic: CANAG
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: 10 cc clotted blood
Container Required: red top tube
Volume of Specimen: 2 ml serum
Availability: Specimens will be processed Mon-Fri 7:00AM-3:30PM
Time Required Routinely for Processing: 72 hours
Reference Range: Negative
Chlamydia PCR
General Use: Identification of Chlamydia trachomatis by PCR. NOTE: Culture
is the method of choice in cases of suspected sexual abuse, ascending pelvic
infections, throat or rectal infections or when determining test of cure.
Test Mnemonic: CTPCR
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Vaginal / endocervical or urine
Container Required: xpert CT/NG vaginal/endocervical specimen collection
kit, for urine use a sterile cup or yellow cap urinalysis tube
Volume of Specimen: 1 swab in special transport vial, Urine –Min. 1 ml
Causes for Rejection: Specimen not received in appropriate transport media.
Inappropriate specimen. Specimen CANNOT be shared with any other tests.
Availability: Testing performed 7 days/week ( first shift ONLY)
Time Required Routinely for Processing: 2-3 hours
Reference Range: Negative
Clostridium Difficile Toxin, Stool for C.Diff see C. Difficile Toxin
Cryptococcal Antigen, Blood
General Use: Aid in the diagnosis and prognosis of cryptococcal infections
Test Mnemonic: CRYBLD
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: 10 cc of clotted blood
Volume of Specimen: 2 ml serum
Availability: 7:00AM-3:00PM Mon-Fri; not Stat
Time Required Routinely for Processing: 24 hrs
Reference Range: Negative
Cryptococcal Antigen, CSF
General Use: Aid in the diagnosis and prognosis of cryptococcal infections
Test Mnemonic: CRYCSF
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Spinal fluid
Container Required: Sterile tube
Minimum Volume of Specimen: 2mL
Causes for Rejection: Blood in CSF or inadequate volume
Availability: 7:00AM-3:00PM Mon-Fri; as a Stat only 3:00PM-11:00PM when
indicated by diagnosis (if positive, titer will be done next routine day)
Time Required Routinely for Processing: 24 hrs
Reference Range: Negative
Cryptococcus Stain see India Ink Prep
Cryptosporidum Antigen
General Use: Determine presence or absence of Cryptosporidium as cause of
infection.
Test Mnemonic: CRYPT
Lab Performing Test: Sent to reference Lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool
Container Required: Plastic urine or stool container
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Insufficient quantity, contamination with urine, mineral
oil. etc.; less than 24 hours between duplicate specimens.
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: results available within 72 hrs.
Reference Range: Negative
Culture, Anaerobic
General Use: To identify infection due to anaerobic organisms
Test Mnemonic: ANER
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate type of specimen & site; see general information
for unacceptable specimens
Specimen Required: Swab of wound, drainage, etc., biopsy material, pus.
Specimen for anaerobic culture should be accompanied by a specimen for aerobic
culture from the same site.
Container Required: Anaerobic transport tube (available from Microbiology
Laboratory)
Volume of Specimen: Small piece of tissue from biopsy site, 1 swab, 1-10 ml of
fluid, etc.
Causes for Rejection: Specimen not received in appropriate transport tube or
vial. Refrigerated specimens (refrigeration inhibits viability of anaerobic
organisms). Specimens from sites which have anaerobic bacteria as normal flora
will routinely be rejected, e.g., throat, rectal swabs, cervical-vaginal mucosal
swabs, sputums. etc.
Availability: Cultures will be processed daily 7:00AM - 10:00PM
Time Required Routinely for Processing: Cultures showing no bacterial growth
will be reported after 5 days. Complete reports of cultures with anaerobic bacteria
may take as long as 10 days to 2 weeks after receipt of culture depending upon the
nature of the organisms isolated.
Reference Range: No growth of anaerobes from normally sterile sites.
Culture, Candida
Synonym: Yeast Culture, Monilia culture
General Use: To support or rule out Candida (yeast) infection
Test Mnemonic: CANDC
Test Includes: Screening for Candida species
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Swab of wound, drainage, genital area, etc
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Received in improper container
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Cultures will be held 3 days before
going out as negative; additional time may be needed for identification if growth
of yeast occurs
Reference Range: No growth
Culture, Anal/Rectal for GC
General Use: Support diagnosis of Neisseria gonorrhea as cause of infection
Test Mnemonic: ANALGC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Deliver to lab immediately; do not refrigerate.
Specimen Required: Anal swab/Rectal swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Refrigeration
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Cultures with no growth will be
reported after 48 hours.
Reference Range: No Growth of Neisseria gonorrhoeae
Culture, Blood – Bacterial
Synonyms: Blood Culture, Routine; Blood Culture Fungal
General Use: Isolate and identify causative agent of infection. If a fungal isolate
is suspected, the microbiology lab MUST be notified.
Test Mnemonic: BLC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: After selecting a vein, the arm should be wiped thoroughly
with 70% alcohol and allowed to dry. It is then wiped with an iodophore swab (or
2% tincture of iodine) and allowed to dry for 1 minute. The venipuncture site
must not be palpated after the above steps. 10 ml of venous blood is then drawn
into two blood culture bottles which have been properly labeled, exercising
extreme caution not to inject air into the anaerobic bottle.
Special Instructions: Should be drawn prior to administration of antibiotics and
before expected temperature rise.
Specimen Required: Blood
Container Required: 2 special blood collection bottles (aerobic and anaerobic)
Volume of Specimen: 10 ml (5ml in each bottle) - 20 ml (10 ml in each bottle)
Minimum Volume of Specimen: 2 ml (1 ml in each bottle)
Causes for Rejection: Insufficient volume
Availability: Specimens will be processed daily 7:00AM-11:00PM and drawn as
a Stat 24 hours daily
Time Required Routinely for Processing: Routine Blood Cultures are held for 5
days if negative; Fungal Blood Cultures are held 21 days if negative.
Reference Range: No Growth
Culture, Body Fluid
General Use: Determine causative agent of infection
Test Mnemonic: FLDC
Test Includes: A gram stain if sufficient quantity of fluid is received and not
collected by swab.
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Sterile preparation of the aspiration site
Special Instructions: Indicate type of body fluid
Specimen Required: Sterile aspirated body fluid
Container Required: Sterile tube or culturette - do not submit in vacutainer tubes
.
Volume of Specimen: 2.0 ml; 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container
Availability: Specimens will be processed daily 7:00AM-11:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be reported after 5 days.
Reference Range: No growth
Culture, Bone
General Use: To determine presence of infection
Test Mnemonic: BONC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Bone
Container Required: Sterile cup
Volume of Specimen: As much as can be obtained
Availability: Specimens will be processed daily 7:00AM-11:00PM
Time Required Routinely for Processing: Specimens will be held for 5 days
before reporting as negative
Reference Range: No growth
Culture, Bone Marrow
General Use: To support or rule out infection
Test Mnemonic: BMC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: As required for Bone Marrow Aspiration
Special Instructions: Media may be obtained in Microbiology Lab
Specimen Required: Bone Marrow
Container Required: Planted on proper media at bedside (Thio Broth)
Volume of Specimen: 1 ml
Availability: Specimens will be processed daily 7:00AM-3:30PM
Time Required Routinely for Processing: Specimens will be held for 5 days
before reporting as negative
Reference Range: No growth
Culture, Bone Marrow for AFB see Culture, Mycobacteria, Bone Marrow
Culture, Bronchial Washing
General Use: Determine causative agent of infection
Test Mnemonic: BRWC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Bronchial washings, bronchial brushings, bronchial lavage
Container Required: Sterile cup or sterile bronchscopy tube (be sure to keep
from leaking)
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Specimen not received in appropriate sterile container.
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; Cultures with no growth will be reported after 48 hours; reports on
specimens from which pathogens are isolated require a minimum of 48 hours for
completion.
Reference Range: No growth or growth consistent with normal respiratory flora
Culture, Catheter Tip
General Use: To determine causative agent of infection
Test Mnemonic: CTC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Catheter tip (must specify type of catheter tip)
Container Required: Sterile tube
Volume of Specimen: 1-5 cm catheter tip
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be reported after 3 days
Reference Range: No growth
Culture, CMV
General Use: To support or rule out CMV infection
Synonyms: Culture, Cytomegalovirus
Test Mnemonic: CMVC
Test Includes: CMV early antigen screen & CMV cell culture
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Urine must be received in early AM on ice; must be
transported to reference lab (on ice) same day as collected. Blood must be put on
ice after collection.
Specimen Required: Urine, heparinized blood; contact Micro for specific
requirements
Container Required: Sterile cup; green top tube; call Micro for other
requirements
Volume of Specimen: 2 ml urine; 10 cc blood; call Micro for other requirements
Causes for Rejection: Specimen not received on ice; specimen received after
courier pick up
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: Early antigen available in 72 hours;
culture held 8 weeks if negative
Reference Range: Negative
Culture, CSF for AFB see Culture, Mycobacteria, CSF
Culture, CSF & Gram Stain
General Use: Determine causative agent of infection.
Test Mnemonic: CSFC
Test Includes: CSF culture as well as a gram stain on all specimens submitted
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Sterile preparation of the aspiration site
Specimen Required: Spinal fluid, shunt fluid
Container Required: Sterile tube
Volume of Specimen: 2-5 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Specimen not received in appropriate sterile container.
Availability: Specimens will be processed daily 7:00AM-11:00PM and planted
as a STAT culture 11:00PM-7:00AM (culture will be planted and gram stain will
be made and stained.)
Time Required Routinely for Processing: Preliminary reports available at 24
hours; Cultures with no growth will be reported after 72 hours; reports of cultures
from which pathogens are isolated may require additional time for completion.
Reference Range: No growth
Culture, Cytomegalovirus see Culture, CMV
Culture, Ear
General Use: To determine causative agent of infection
Test Mnemonic: EARC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Ear swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; Cultures with no growth will be reported after 72 hours
Reference Range: No growth
Culture, Eye
General Use: Determine causative agent of infection
Test Mnemonic: EYEC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Eye swabbing, conjunctiva
Special Instructions: DO NOT REFRIGERATE
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; Cultures with no growth will be reported after 72 hours
Reference Range: No growth
Culture, Fungus
General Use: To support or rule out fungal infection
Test Mnemonic: FUNG
Test Includes: Isolation and identification of yeasts and pathogenic fungi
Lab Performing Test: Sent to Reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Same as for routine culture of specific site
Specimen Required: Swab of wound, drainage, biopsy material, fluid, pus, etc.
Container Required: Sterile culturette; sterile cup or tube
Volume of Specimen: 1 swab, 2-3 ml fluid
Causes for Rejection: Insufficient specimen volume, received in improper
container
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: Cultures will be held 15 days before
going out as negative; growth of fungus may take additional time for
identification. Note: some fungus species may take as long as one month to grow;
if dermatophytes are suspected the lab should be notified
Reference Range: No growth
Culture, Fungus, Bone Marrow
General Use: To support or rule out fungal infection
Test Mnemonic: FUNG
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: As required for bone marrow aspiration
Special Instructions: Media may be obtained in Microbiology Lab
Specimen Required: Bone marrow
Container Required: Planted on proper media at bedside (SAB agar slants)
Volume of Specimen: 2 ml
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: Specimens will be held 15 days
before going out as negative; growth of fungus may take additional time for
identification
Reference Range: No growth
Culture, Fungus, Skin
Synonyms: Skin scrapings for fungus
General Use: To support or rule out fungal infection
Test Mnemonic: FUNG
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Wash area with alcohol swab. Allow to dry and scrape with
blade or edge of glass slide
Specimen Required: Skin scrapings, exudates, nail clippings, whole nail, debris
under nail
Container Required: Sterile petri dish
Volume of Specimen: As much as possible
Causes for Rejection: Insufficient specimen volume
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: 21 days. Growth of fungus may take
additional time.
Reference Range: No growth
Culture, Genital Sites
General Use: Determine causative agent of infection
Test Mnemonic: GENC
Test Includes: Screening for Beta Strep Group B, Listeria, Neisseria
gonorrhoeae and predominating amounts of Gardnerella vaginalis and candida
species unless otherwise indicated.
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: DO NOT REFRIGERATE
Specimen Required: Swab of genital area, prostatic fluid, placental swab, etc.
Container Required: Sterile culturette or sterile tube
Volume of Specimen: 1 swab or 2 ml fluid
Causes for Rejection: Specimen not received in appropriate sterile container;
refrigerated specimens.
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be reported after 72 hours; reports of cultures
from which pathogens are isolated may require additional time for completion.
Reference Range: No growth or normal genital flora depending on site
Culture, Legionella
Synonyms: Legionella Culture
General Use: To establish Legionella species as cause of infection
Test Mnemonic: LEGC
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Sputum, bronchial washings
Container Required: Sterile cup
Volume of Specimen: 2 ml
Availability: Specimens will be processed Mon-Thur. 7:00AM-3:00PM.
Specimens received after 10:00am on Friday or Saturday, Sunday or holidays
will be processed on the next routine day.
Time Required Routinely for Processing: 2 weeks
Reference Range: Negative
Culture, Mycobacteria
Synonyms: AFB Smear and Culture, MAV Culture, TB Culture
General Use: Determine presence or absence of Mycobacteria
Test Mnemonic: AFB
Test Includes: DNA probe identification of mycobacteria when present
Lab Performing Test: Microbiology to perform concentrated smear ony as stat.
All others sent to reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Indicate type of specimen on requisition. If gastric aspirate
is to be collected, the Microbiology Department must be notified before
collection.
Specimen Required: Sputum, fasting gastric aspirate, induced sputum, Bronchial
washing, wound, body fluid, stool, etc.
Container Required: Sterile cup
Volume of Specimen: 5 - 10 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Specimen consisting entirely of saliva, insufficient
specimen volume
Availability: Specimens processed daily 7:00AM-10:00PM.. Concentrated AFB
smears will be performed inhouse if clinically indicated (Inpatient). Otherwise
sent to reference lab.
Time Required Routinely for Processing: Cultures are held 6 weeks for
Negative.
Reference Range: No Growth of Mycobacteria
Culture, Mycobacteria, Blood
Synonyms: Blood Culture for AFB; Blood Culture for MAV; AFB Blood
Culture;
MAV Blood Culture
General Use: To support the diagnosis of AFB
Test Mnemonic: BLAFB
Test Includes: DNA probe identification of Mycobacteria when present
Lab Performing Test: Microbiology (initial incubation). If positive sent to
reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Bottles for drawing specimens may be obtained in
Microbiology
Specimen Required: Blood
Container Required: MB/Bact Blood culture Bottle
Volume of Specimen: 5 ml
Collection: Timed; usually drawn x2
Causes for Rejection: Insufficient volume; improper container
Availability: 7:00AM-11:00PM. for routine processing; will be drawn 24 hours
daily by request.
Time Required Routinely for Processing: 6 weeks
Reference Range: No growth of Mycobacteria
Culture, Mycobacteria, Bone Marrow
Synonyms: Culture, Bone Marrow for AFB
General Use: Determine presence or absence of Mycobacteria
Test Mnemonic: AFB
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: As required for bone marrow aspiration
Special Instructions: Media may be obtained in Microbiology Lab
Specimen Required: Bone Marrow
Container Required: Planted on proper media at bedside (LJ slant)
Volume of Specimen: 2 ml
Availability: Specimen will be processed daily 7:00AM-3:30PM
Time Required Routinely for Processing: 6 weeks
Reference Range: No Growth of Mycobacteria
Culture, Mycobacteria, CSF
Synonyms: Culture, CSF for AFB
General Use: Determine presence or absence of Mycobacteria
Test Mnemonic: AFB
Lab Performing Test: To reference lab.
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Usual sterile preparation
Special Instructions: Indicate "CSF" as source
Specimen Required: Cerebrospinal fluid
Container Required: Sterile tube
Volume of Specimen: 1-10 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Insufficient specimen volume
Availability: Not Stat, specimens processed daily 7:00AM-11:00PM
Time Required Routinely for Processing: 6 weeks . Direct smear available in
24 hours
Reference Range: No Growth of Mycobacteria
Culture, Mycoplasma Pnemoniae
General Use: To determine causative agent of infection
Test Mnemonic: MYCUL
Test Includes: Culture for Mycoplasma pneumoniae
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Must be kept refrigerated until receipt and frozen if > 24
hour delay in transport
Specimen Required: Respiratory – throat, sputum.
Container Required: Special holding media available in Microbiology
Volume of Specimen: 1 swab
Causes for Rejection: Received in inappropriate container; not refrigerated
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday or Sat. Sun., or holidays will be
processed on the next routine day.
Time Required Routinely for Processing: 8 weeks
Reference Range: Negative
Culture, Mycoplasma/Ureaplasma
General Use: To determine causative agent of infection
Test Mnemonic: MYURC
Test Includes: Culture for Mycoplasma hominis & Ureaplasma urealyticum
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Must be kept refrigerated until receipt and frozen if >24
hour delay in transport.
Specimen Required: urogenital, respiratory, sterile body fluids, tissue, wounds
Container Required: Special holding media available in Microbiology
Volume of Specimen: 1 swab
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday or Sat. Sun., or holidays will be
processed on the next routine day.
Time Required Routinely for Processing: 6 weeks
Reference Range: Negative
Culture, Nose
General Use: Determine causative agent of infection
Test Mnemonic: NOSC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Nose swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Specimen not received in appropriate sterile culturette.
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; Cultures with no growth will be reported after 48 hrs.
Reference Range: Alpha strep, Diphtheroids, Neisseria sp. Staphylococcus
epidermidis are all normal flora
Culture, Pertussis
Synonyms: Whooping Cough
General Use: Used to support the diagnosis of Bordetella pertussis (Whooping
Cough)
Test Mnemonic: PERTC
Test Includes: Culture for B.pertussis
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Nasopharyngeal swab from Pertussis collection kit;
transport to lab immediately.
Container Required: Pertussis kit suplied by lab
Volume of Specimen: 1 swab from kit
Availability: Specimens will be processed daily 7:00AM to 10:00PM.
Time Required Routinely for Processing: If received Mon-Fri by noon, culture
held 5 days.
Reference Range: Negative
Culture, Skin
General Use: To determine causative agent of infection
Test Mnemonic: SKIN
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Usual sterile preparation
Specimen Required: Swab of skin; indicate whether deep specimen or
superficial
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; Cultures with no growth will be reported after 48 hours.
Reference Range: No growth
Culture, Sputum and Gram Stain
General Use: Determine causative agent of infection
Test Mnemonic: SPUC
Test Includes: Sputum culture as well as a gram stain on all specimens submitted
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: The patient should be instructed to remove dentures, rinse
mouth and gargle with water. The patient should then be instructed to cough
deeply and expectorate sputum into proper container.
Specimen Required: Sputum, first morning specimen preferred
Container Required: Sterile cup
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Specimen not received in appropriate sterile container or
received in tissue paper; contamination with mouthwash. If specimen is
macroscopically/ microscopically consistent with saliva it will be rejected and a
new specimen will be requested.
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be finaled after 48 hours.
Reference Range: Normal upper respiratory flora
Culture, Stool
Synonyms: Stool for Enteric Pathogens
General Use: Screen for bacterial pathogens in the stool
Test Mnemonic: STLC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Rectal swab or fresh random stool; ideally stool should be
received within 2 hours of passage
Container Required: Sterile culturette or stool cup
Volume of Specimen: 10 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Rectal swabs in which the medium compartment is not
broken; improper container, insufficient specimen volume, specimen
contaminated with urine and/or water, specimen containing interfering substances,
e.g. castor oil, bismuth, metamucil, barium; specimens greater than 24 hrs old
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: 48 hrs if negative.
Additional Information: A routine stool culture includes screening for Salmonella,
Shigella, Campylobacter & E coli 0157 only. If other enteric pathogens such as
Yersinia or Vibrio species are suspected, testing must be specifically requested.
Reference Range: No growth of enteric pathogens
Culture, Throat (Comprehensive)
General Use: Determine causative agent of infection
Test Mnemonic: THRC
Test Includes: Screening for respiratory pathogens Group A Strep, Haemophilus
influenzae and Streptococcus Pneumoniae.
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Avoid contamination with tongue or sides of mouth
Specimen Required: Throat swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container
Availability: Specimens will be processed daily 7:00AM to 10:00PM.
Time Required Routinely for Processing: up to 48 hours for a negative result.
Reference Range: Normal upper respiratory flora
Culture, Throat Beta Strep Group A
Synonyms: Beta Strep Screen
General Use: Rule out Beta Strep Grp. A
Test Mnemonic: BSSC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Throat swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container
Availability: Specimens will be processed daily 7:00AM to 10:00PM.
Time Required Routinely for Processing: up to 48 hours for a negative result. A
10 MINUTE RAPID RESULT IS AVAILABLE 24 HOURS DAILY, and must
be clearly stated on the requisition as “10 minute Beta Strep”.
Reference Range: No Beta strep isolated or negative by rapid screen
Culture, Throat, for C. Diphtheria
General Use: Rule out C. diphtheria as causative agent of infection
Test Mnemonic: MISC
Lab Performing Test: CT State Laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Contact Microbiology and Infection control prior to
collection.
The preferred diagnostic test is to send acute and convalescent serum for
diphtheria antibody titers to the State Lab. Secondary testing may include
histological and microbiological testing of the pseudomembrane.
Culture, Throat, for Neisseria Gonorrhoeae
Synonyms: G.C. Culture, Throat
General Use: Rule out Neisseria gonorrhoeae as causative agent of infection
Test Mnemonic: THRC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Transport specimen immediately; hand carry to laboratory;
do not refrigerate. Additional description field must indicate "for Neisseria
Gonorrhoeae"; DNA Probe testing is NOT approved for use on throat cultures
Specimen Required: Throat swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container;
refrigeration of specimen
Availability: Specimens will be processed daily 7:00AM to 10:00PM
Time Required Routinely for Processing: 48 hours for negative
Reference Range: No Neisseria gonorrhoeae isolated
Culture, Tissue
General Use: To determine cause of infection
Test Mnemonic: TISC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Hip capsule, knee capsule, lymph node, other body tissues
Container Required: Sterile cup, tube or petri dish hand delivered to the lab,
must be kept upright to keep from leaking
Volume of Specimen: Amount of tissue available depending on source
Causes for Rejection: Specimen leaking into bag (since blood may leak back
into petri dish cultures may be contaminated)
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary results available after 24
hours. Negative cultures are held for 5 days
Reference Range: No growth
Culture, Urine, Catheterized
General Use: To determine causative agent of urinary tract infection.
Test Mnemonic: URNC
Test Includes: Colony count
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: As per Nursing Procedure Manual for catheterization.
Special Instructions: Specimen must be refrigerated if not immediately taken to
laboratory to be processed. Additional description field must indicate type of
catheter collection method ie; “Straight Catheter” or “Foley Catheter”.
Specimen Required: Catheterized Urine Specimen
Container Required: Sterile tube or sterile urine container
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Specimen not received in appropriate sterile container,
refrigerated specimen older than 24 hours. Non-refrigerated specimen older than 2
hours.
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be reported after 24 hours. Cultures with
significant growth will require a minimum 48 hours for completion.
Reference Range: No Growth
Culture, Urine Midvoid Specimen
General Use: To determine causative agent of urinary tract infection
Test Mnemonic: URNC
Test Includes: Colony count
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Thoroughly instruct patient for proper collection of midvoid urine specimen to avoid contamination with skin flora. Refer to Nursing
Procedures for further instructions.
Special Instructions: Specimen must be refrigerated if not immediately taken to
laboratory to be processed. Additional description field should indicate, "clean
voided"
Specimen Required: Clean void urine specimen
Container Required: Sterile tube or sterile urine container
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Specimen not received in appropriate sterile container;
refrigerated specimen older than 24 hours; non-refrigerated specimen older than 2
hours
Availability: Specimens will be processed daily 7:00AM-11:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be finaled after a full 24 hours. Cultures with
growth require a minimum of 48 hours to completion
Reference Range: No growth
Culture, Urine, Suprapubic Puncture
General Use: To determine causative agent of urinary tract infection.
Test Mnemonic: URNC
Test Includes: Colony count
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Specimen to be obtained by suprapubic puncture so as to
avoid any contamination with normal skin flora.
Special Instructions: Specimen should be immediately taken to laboratory to be
processed. Additional description field must indicate "suprapubic puncture"
Specimen Required: Urine obtained by suprapubic puncture
Container Required: Sterile tube or sterile urine container
Minimum Volume of Specimen: 2 ml
Causes for Rejection: Specimen not received in appropriate sterile container;
refrigerated specimen older than 24 hours; non-refrigerated specimen older than 2
hours
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: : Preliminary reports available at 24
hours; cultures with no growth will be finaled after a full 24 hours. Cultures with
growth require a minimum of 48 hours to completion
Reference Range: No growth
Culture, Wound, Miscellaneous Sites
General Use: Determine causative agent of infection.
Test Mnemonic: WDC
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Usual sterile preparation
Special Instructions: Indicate type of specimen & site
Specimen Required: Swab of wound, drainage, etc.; biopsy material; pus
Container Required: Sterile tube, sterile culturette
Volume of Specimen: 2.0 ml, 1 swab
Causes for Rejection: Specimen not received in appropriate sterile container
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Preliminary reports available at 24
hours; cultures with no growth will be reported after 72 hours; reports on
specimens from which pathogens are isolated require a minimum of 48 hours for
completion.
Reference Range: No growth
Cyclospora Stain
General Use: To rule out infection due to Cyclospora
Test Mnemonic: CYCLO
Lab Performing Test: to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool
Container Required: Plastic urine or stool container
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Insufficient quantity; contamination with urine, mineral
oil, etc.; less than 24 hours between duplicate specimens
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: Mon - Friday by 11:00AM will be
completed that same day.
Reference Range: Negative
PCR for Neisseria Gonorrhoeae
Synonyms: GC PCR
General Use: Identification of Neisseria gonorrhoeae by DNA Probe.
NOTE: Culture is the method of choice in cases of suspected sexual abuse,
ascending pelvic infections, throat or rectal infections or when determining test of
cure.
Test Mnemonic: GCPCR
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Genital swab
Container Required: xpert CT/GC media (available in Microbiology)
Volume of Specimen: 1 swab in special transport vial
Causes for Rejection: Specimen not received in appropriate transport media;
sources not appropriate for this test.
Availability: Testing performed 7 days/week ( first shift ONLY)
Time Required Routinely for Processing: 2-3 hours
Reference Range: Negative
Enterovirus PCR (CSF)
General Use: To screen for Enterovirus CSF
Test Mnemonic: ENVCSF
Test Includes: Screening for ENTEROVIRUS ONLY
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: NASAL swab
Container Required: Cepheid Copan Red top swab
Volume of Specimen: 1 swab
Causes for Rejection: inappropriate swab
Availability: 24/7
Time Required Routinely for Processing: 2-3 hours
Additional Information: Presumptive positive cultures will be called as soon as
possible
Reference Range: Negative
Fetal Fibronectin Test
Synonyms: FFN
General Use: To predict the risk for preterm delivery
Test Mnemonic: FFN
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: As for a sterile speculum examination
Special Instructions: Specimens for FFN should be collected prior to a digital
cervical exam and before collection of any culture specimens
Specimen Required: Swab of the posterior fornix of the vagina
Container Required: Special FFN swab and holding media ONLY
Volume of Specimen: 1 FFN swab (available in Microbiology)
Collection: by physician
Causes for Rejection: Inappropriate container; delay in transport to lab
Availability: 24 hours daily
Time Required Routinely for Processing: 2 hours
Limitations: Vaginal bleeding may contribute to difficulty in interpreting the
FFN result
Additional Information: Specimens must be delivered to Microbiology
immediately after collection. If there will be any delay in transport, the specimen
should be refrigerated. Transport from outside the hospital should be on an ice
pack.
Fungal Wet Prep see KOH Prep
GBS Screen see Group B Strep Screen
G.C. Culture, Throat see Culture, Throat, for Neisseria Gonorrhoeae
GC DNA Probe see DNA Probe for Neisseria Gonorrhoeae
Giardia Antigen
General Use: Determine presence or absence of Giardia lamblia as cause of
infection.
Test Mnemonic: GLAG
Lab Performing Test: To reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool
Container Required: Sterile cup, stool container
Volume of Specimen: 5-10 ml
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Received older than 24 hrs, received in container lined
with tissue or newspaper
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: 72 hours
Reference Range: Negative
Gram Stain
General Use: To determine presence or absence of bacteria, yeast, neutrophils
and epithelial cells.
Test Mnemonic: GRAM
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Same as for routine culture of specific site.
Specimen Required: Same specimen as for routine culture of the specific site.
Container Required: Sterile tube, sterile culturette
Minimum Volume of Specimen: 1 ml, or one swab
Causes for Rejection: Specimen not received in appropriate sterile container.
Stool specimens are not appropriate for gram staining. See stool for Ploys.
Availability: Specimens will be processed daily 7:00AM-10:00PM.
Time Required Routinely for Processing: 24 hours
Group B Strep Screen (Culture/PCR)
Synonyms: GBS Screen
General Use: For GBS screening in pregnant women
Test Mnemonic: GBS
Test Includes: Screening for Beta Strep Group B ONLY
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Collect one or two swabs of vaginal introitus AND
anorectum
Specimen Required: Vaginal-rectal swabs are the preferred specimen
Container Required: sterile culturette
Volume of Specimen: 1 or 2 swabs
Causes for Rejection: Inappropriate container, improper specimen
Availability: Specimens will be processed daily 7:00AM-10:00PM
Time Required Routinely for Processing: Cultures are held a total of 72 hours
before being reported as negative
Additional Information: It is recommended that both vaginal and rectal cultures
be taken; the combination can increase sensitivity 5 - 27% over vaginal culture
alone. Cervical cultures are not acceptable for GBS screening
Reference Range: Negative for Group B Strep
India Ink Prep
Synonyms: Cryptococcus Stain
General Use: Determine the presumptive presence of Cryptococcus sp.
Test Mnemonic: INKP
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Same as for routine culture of specific site
Specimen Required: Appropriate specimen is the same as for culture of a given
site. Most commonly done on CSF
Container Required: Sterile tube
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Insufficient specimen volume
Availability: 7:00AM to 10:00PM daily
Time Required Routinely for Processing: 2 hours Stat; next routine working
day if received after 10:00PM
Additional information: Cryptococcal Antigen detection test is a more sensitive
and specific test and is recommended instead of the India Ink prep on CSF
specimens.
Reference Range: Negative
Influenza PCR
Synonym: Flu Test
General Use: To establish Influenza A/B as cause of infection
Test Mnemonic: FLUPCR
Test Includes: Screening for Influenza A+B , H1N1
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Only swabs and transport media approved for test can be
accepted - obtained from the Microbiology Lab.
Specimen Required: Nasopharyngeal washes/aspirates or swabs recommended;
throat swabs will be accepted but are not preferable.
Volume of Specimen: 1 swab
Causes for Rejection: Improper specimen or container. Received greater than 24
hours after collection
Availability: 24/7
Reference Range: Negative for Influenza A/B antigen
KOH Prep
Synonyms: Fungal Wet Prep
General Use: Determine presence of fungal elements
Test Mnemonic: KOHP
Lab Performing Test: To reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Same as for routine culture of specific site
Specimen Required: Appropriate specimen for KOH preparation is the same as
for fungus culture
Container Required: Sterile tube
Volume of Specimen: 2 ml sputum or fluid: skin scrapings
Causes for Rejection: Insufficient specimen volume, dry swab
Availability: Performed daily 7:00AM-9:00PM
Time Required Routinely for Processing: Specimens will be processed Monthurs. 7:00AM-3:30PM Specimens received after noon on Friday, Sat. Sun., or
holidays will be processed on the next routine day.
Reference Range: No yeast or fungal elements
Legionella Culture see Culture, Legionella
Legionella DFA
General Use: To support diagnosis of Legionella infection
Test Mnemonic: LDFA
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Sputum, bronchial washings
Container Required: Sterile cup
Volume of Specimen: 2 ml
Causes for Rejection: Specimen consistent with saliva
Availability: Specimens will be processed Mon-Thurs. 7:00AM-2:00PM.
Specimens received on Fridays after 12 noon or on Sat, Sun or holidays will be
processed on the next routine day.
Time Required Routinely for Processing: 48 – 72 hours.
Reference Range: Negative
Legionella Urine Antigen
General Use: To support diagnosis of Legionella infection
Test Mnemonic: LEGUR
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Clean void urine (keep refrigerated)
Container Required: Sterile cup or tube
Volume of Specimen: 10-20 ml
Minimum Volume of Specimen: 10 ml
Availability: Specimens will be processed Mon-Fri 7:00AM-3:30PM
Time Required Routinely for Processing: Specimens received by 11:00AM will
be complete the same day
Reference Range: Negative
MAV Blood Culture see Culture, Mycobacteria, Blood
Maximum Bactericidal Dilution (MBD)
Synonyms: Schlicter Test; Serum Cidal Test; MBD
General Use: To determine the greatest dilution of the serum or body fluid which
will kill the patient's infecting organism
Test Mnemonic: MBD
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: If a maximum bactericidal dilution test is desired, the
physician must request the laboratory to save the patient's isolate (if the isolate
has not been saved, the test cannot be performed.)
Specimen Required: 10 cc of clotted blood and the saved isolate
Container Required: Red top tube; sterile tube for body fluid
Volume of Specimen: 10 cc of clotted blood or 2 ml of body fluid in a sterile
tube
Minimum Volume of Specimen: 2 ml serum
Causes for Rejection: Insufficient amount of serum or fluid; specimen not
received in appropriate sterile container: specimen older than 4 hours: no request
to save organism
Availability: Specimens will be processed daily 7:00 a.m. to 3:30 p.m.; blood
will be drawn any time required
Time Required Routinely for Processing: At least 72 hours
MBC see Minimum Bactericidal Concentration
MBD see Maximum Bactericidal Dilution (MBD)
MIC see Minimum Inhibitory Concentration (Send Out Test)
Minimum Bactericidal Concentration
Synonyms: MBC
General Use: To determine Minimum lethal concentration of antimicrobial agent
Test Mnemonic: MBCSO
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: The patient's infecting organism must be save at the request
of the physician (if the isolate has not been saved, the test cannot be performed)
Specimen Required: Isolation plate of organism to be tested (prepared by the
Microbiology Laboratory)
Container Required: Agar plate or tube
Volume of Specimen: NA
Causes for Rejection: Organism discarded prior to request by physician to save
the organism for MBC
Availability: Send out Mon – Thur. 2PM. Friday specimens must be submitted
by 10:00 AM.
Time Required Routinely for Processing: At least 72 hours
Minimum Inhibitory Concentration (Special request Send Out Test)
Synonyms: MIC
General Use: To determine susceptibility of a given organism to an antimicrobial
agent
Test Mnemonic: MICSO
Lab Performing Test: Sent to reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: The patient's infecting organism must be saved at the
request of the physician
Specimen Required: Isolation plate of organism to be tested (prepared by the
Microbiology Laboratory)
Container Required: Agar plate or tube
Volume of Specimen: NA
Causes for Rejection: Organism discarded prior to request by physician to save
the organism for MIC
Availability: Send out Mon – Thurs. 2PM. Friday specimens must be submitted
by 10:00 AM.
Time Required Routinely for Processing: At least 72 hours
MRSA Screen PCR
General Use: To screen for MRSA Colonization
Test Mnemonic: MRSAN
Test Includes: Screening for MRSA ONLY
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: NASAL swab
Container Required: Cepheid Copan Red top swab
Volume of Specimen: 1 swab
Causes for Rejection: inappropriate swab
Availability: 24/7
Time Required Routinely for Processing: 2-3 hours
Additional Information: Presumptive positive cultures will be called as soon as
possible
Reference Range: Negative
MRSA/SA Screen PCR (soft tissue)
General Use: To screen for MRSA
Test Mnemonic: STAPHC
Test Includes: Screening for MRSA and Staph aureus
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: tissue swab
Container Required: Cepheid Copan Red top swab
Volume of Specimen: 1 swab
Causes for Rejection: inappropriate swab
Availability: 24/7
Time Required Routinely for Processing: 2-3 hours
Additional Information: Presumptive positive cultures will be called as soon as
possible
Reference Range: Negative
O& P, Stool for Amoeba, Worm Identification see Ova and Parasites
Occult Blood, Guaiac Method
Synonyms: Stool for Blood; Stool for Guaiac
General Use: Routine screening procedure for occult blood in stool, etc.
Test Mnemonic: OBLD
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Patient should not receive vitamin C (ascorbic acid) for 3
days prior to occult blood testing; patient should also refrain from eating red meat
the day prior to testing.
Specimen Required: Stool, vomitus, gastric asp
Container Required: Sterile cup; stool cup
Minimum Volume of Specimen: 5 ml
Causes for Rejection: Specimen received on swab or wooden applicator
Availability: Testing routinely done Mon - Fri afternoon unless requested
verbally as STAT
Time Required Routinely for Processing: 1 hr
Reference Range: Negative or trace
Pinworm Exam
Synonyms: Scotch tape prep; Pinworm Prep
General Use: To detect cases of enterobiasis. One negative result does not rule
out the possibility of parasitic infection
Test Mnemonic: PINW
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Adhesive slide paddle obtained from the microbiology lab;
if scotch tape is used, it must be TRANSPARENT tape placed on a Clear glass
slide for transport.
Volume of Specimen: 1 slide
Container Required: Adhesive pinworm paddle (available in Microbiology)
Availability: 7:00AM – 11:00AM Mon-Fri
Time Required Routinely for Processing: If specimen is received by 11 am it
will be complete the same day
Causes for Rejection: specimens received on opaque tape; specimens not
obtained/transported properly.
Reference Range: No pinworm (Enterobius vermicularis) eggs seen
Pinworm Prep see Pinworm Exam
Pneumocystis DFA
Synonyms: Sputum for PCP
General Use: Used to support the diagnosis of Pneumocystis pneumonia
Test Mnemonic: PCPT
Lab Performing Test: reference laboratory
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Sputum must be collected by respiratory therapist to obtain
induced specimen
Specimen Required: induced Sputum; Bronchoalveolar lavage; Bronchial
washings. Routinely expectorated sputum is NOT acceptable
Container Required: Sterile Cup
Volume of Specimen: 2-3 ml
Minimum Volume of Specimen:
Causes for Rejection: Routinely expectorated Sputum
Availability: Mon-Fri send out at 2PM.
Time Required Routinely for Processing: up to 6 days
Reference Range: Negative
Respiratory Syncytial Virus
Synonyms: RSV Exam
General Use: Used to support the diagnosis of RSV
Test Mnemonic: RSV
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Nasopharyngeal secretions into a mucus extractor
Container Required: Mucus extractor
Volume of Specimen: 0.5 - 1 ml
Availability: 24/7
Reference Range: Negative
Rotavirus Exam
General Use: To support the diagnosis of Rotavirus infection
Test Mnemonic: ROTA
Lab Performing Test: To reference lab
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Stool
Container Required: Sterile cup; stool cup
Volume of Specimen: 2 ml
Availability: Specimens will be processed Mon-thurs. 7:00AM-3:30PM
Specimens received after noon on Friday, Sat. Sun., or holidays will be processed
on the next routine day.
Time Required Routinely for Processing: 24 hrs to 72 hrs
Reference Range: Negative
RSV Exam see Respiratory Syncytial Virus
Schlicter Test see Maximum Bactericidal Dilution (MBD)
Scotch tape prep see Pinworm Exam
Semen Analysis
General Use: Infertility screening
Test Mnemonic: SEMEN
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Patient Preparation: Follow physician's instructions
Specimen Required: Semen
Container Required: Clean dry glass or plastic container
Volume of Specimen: Full amount that is collected
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Refrigerated specimen. Specimen received greater than
one hour after collection; insufficient quantity, improper container.
Availability: ONLY BY APPOINTMENT
Time Required Routinely for Processing: Same Day
Contraindications: If specimen is post vasectomy do not order a complete semen
analysis, order a "sperm post vasectomy"
Reference Range: See report form for individual parameters
Serum Cidal Test see Maximum Bactericidal Dilution (MBD)
Skin scrapings for fungus see Culture, Fungus, Skin
Sperm Post Vasectomy
General Use: As follow up to vasectomy
Test Mnemonic: SPVAS
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Semen
Container Required: Sterile urine cup; clean glass or plastic container
Volume of Specimen: Full amount that is collected
Causes for Rejection: Refrigerated specimen; specimen received >1 hour after
collection, improper container
Availability: Mon - Friday 7:00AM-2:00PM. Specimens must be received by
2:00pm. No Sat, Sun or holiday testing
Time Required Routinely for Processing: Same day
Reference Range: No spermatozoa seen
Sputum for PCP see Pneumocystis DFA
Staph aureus/MRSA Nasal Screen PCR
General Use: To screen for SA/ MRSA Colonization
Test Mnemonic: STAPHC
Test Includes: Screening for MRSA AND Staph aureus
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: NASAL swab
Container Required: Cepheid Copan Red top swab
Volume of Specimen: 1 swab
Causes for Rejection: inappropriate swab
Availability: 24/7
Time Required Routinely for Processing: 2-3 hours
Additional Information: Presumptive positive cultures will be called as soon as
possible
Reference Range: Negative
Stool for Blood see Occult Blood, Guaiac Method
Stool for Enteric Pathogens see Culture, Stool
Stool for Guaiac see Occult Blood, Guaiac Method
Trichomonas Rapid Test
Synonyms: Wet prep for Trichomonas
General Use: To detect Trichomonas
Test Mnemonic: TRICA
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Must be received immediately in 1cc of sterile saline
Specimen Required: Vaginal or urethral swabs
Container Required: Sterile rayon swab from kit
Volume of Specimen:
Causes for Rejection: Inappropriate swab
Availability: 7:00AM-11:00PM daily
Time Required Routinely for Processing: Same day
Reference Range: Negative
Urine for G.C. see Culture, Urine for Neisseria Gonorrhoeae
VRE PCR
General Use: To screen for VRE colonization
Test Mnemonic: VANA
Test Includes: Screening for VRE ONLY
Lab Performing Test: Microbiology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Required: Rectal swab
Container Required: Sterile culturette
Volume of Specimen: 1 swab
Causes for Rejection: No Stool
Availability: Specimens will be processed daily 7:00AM-10:00 PM
Time Required Routinely for Processing: 2-3 hours
Additional Information: Presumptive positive cultures will be called as soon as
possible
Reference Range: Negative
Wet prep for Trichomonas see Trichomonas Prep
Whooping Cough see Culture, Pertussis
URINALYSIS
The Urinalysis section of the Laboratory is directed by Victoria G. Reyes-D'Arcy/ Asim
Ejaz, M.D., D.A.B. Pathologist, and Pedro Ugarelli, M.S., B.S., M.T., Hematology
Manager.
For procedures not listed please consult with the Hematology Manager (Ext. 4137) or
Pathologist.
All specimens must be properly labeled with patient's full name, identification number,
time and date of collection, and the test requested.
Clinitest
General Use: To detect the presence of reducing substances other than glucose
Test Mnemonic: URED
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Special Instructions: Test automatically performed on children less than one
year old when a routine urinalysis is performed
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Urine collection cup or plastic urinalysis tube
Volume of Specimen: 1 ml
Minimum Volume of Specimen: 1 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: Routine 24 hours
Time Required Routinely for Processing: Same day
Reference Range: Negative
Eosinophils in Urine
Synonyms: Urine Eosinophils
General Use: Interpretation by Physician
Test Mnemonic: UEOS
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube or urine collection cup
Volume of Specimen: 8 ml
Minimum Volume of Specimen: 6 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: Daily, not stat
Time Required for Processing: Same day
Reference Range: Negative
EOUR see Eosinophils in Urine
Hemosiderin, Urine
Synonyms: Urine Iron
General Use: Evaluation of hemolytic diseases
Test Mnemonic: UHSDRN
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube or urine collection cup.
Volume of Specimen: 8 ml
Minimum Volume of Specimen: 6 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: Weekdays only, not stat
Time Required Routinely for Processing: Same day
Reference Range: Negative
Routine Urine see Urinalysis - Routine
Urinalysis – Routine
Synonyms: Routine Urine; Routine Urinalysis
General Use: Detection of abnormal urinary chemical, inorganic, or cellular
elements
Test Mnemonic: URIN
Profile Includes: Color, clarity, specific gravity, pH, leukocyte esterase, nitrites,
glucose, ketones, bilirubin, urobilinogen, protein, blood and microscopic cellular
review.
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube.
Volume of Specimen: 6 ml.
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen.
Availability: 24/7
Time Required Routinely for Processing: Stat:30 min; Routine:same day.
Reference Range: See reference ranges for individual tests.
Urine Bilirubin
General Use: To determine the presence of bilirubin in urine
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Patient Preparation: None
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urine tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:30 minutes; Routine: same day.
Reference Range: Negative
Urine Blood
General Use: To determine the presence of hemoglobin in urine
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urine tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:30 minutes; Routine: same day
Reference Range: Negative
Urine Glucose
General Use: To determine the presence of glucose in urine
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Patient Preparation: None
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urine tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat: 30 minutes; Routine: same day
Reference Range: Negative
Urine Iron see Hemosiderin, Urine
Urine Ketones
General Use: To determine the presence of ketones in urine
Lab Performing Test: Hematology
Request Form: Signed order by physician/Health Professional Affiliate(HPA)
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urine tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:30 minutes; Routine:same day
Reference Range: Negative
Urine Leukocyte Esterase
General Use: To screen for the presence of leukocytes in urine
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:30 minutes; Routine:same day
Reference Range: Negative
Urine Nitrites
General Use: To screen for the presence of nitrite-forming bacteria in a random
urine specimen
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Patient Preparation: None
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:30 minutes; Routine: same day
Reference Range: Negative
Urine pH
General Use: To determine the acidity or alkalinity of a random urine specimen;
interpretation by physician
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat: 30 minutes; Routine - same day
Reference Range: 6-7.4
Urine Protein
Synonyms: Random urine protein
General Use: To determine the protein present in a random urine specimen
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required Routinely for Processing: Stat:30 minutes; Routine: same day
Reference Range: Negative
Urine Specific Gravity
General Use: To determine the specific gravity of a random urine specimen;
interpretation by physician
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube
Volume of Specimen: 6 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen.
Availability: 24/7
Time Required for Processing: Stat:30 minutes; Routine: same day.
Reference Range: 1.003 - 1.035
Urine Urobilinogen
General Use: Liver function and hemolytic disease screening
Lab Performing Test: Hematology
Request Form: When this is needed, order Urine test.
Specimen Requirements: Fresh urine. Deliver within 1-2 hours of collection or
maintain specimen refrigerated at 2-8° C.
Container Required: Plastic urinalysis tube
Volume of Specimen: 6 ml
Minimum Volume of Specimen: 3 ml
Causes for Rejection: Incorrect specimen volume, mislabeled or unlabeled
specimen
Availability: 24/7
Time Required for Processing: Stat:30 minutes; Routine: same day
Reference Range: <2.0 Ehrlich units/dL urine