'The new Lamina™ Multilabel Slide Scanner and Vectra® Automated Pathology Imaging
'The new Lamina™ Multilabel Slide Scanner and Vectra® Automated Pathology Imaging System – quantitative pathology solutions for cancer immunology' Bianca Beusink, PhD Imaging Sales Specialist bianca.beusink@perkinelmer.com © 2013 2009 PerkinElmer PerkinElmer Tissue Imaging Portfolio … inForm® Tissue Finder™ inForm® Cell Analysis™ Intelligent Image Analysis Software Lamina™ Slide scanner Nuance™ Multispectral Imaging Systems Vectra® Slide Analysis System … Capturing molecular and spatial complexity, while providing reliable quantitative results Imaging solutions from single cell to live animal… Only PerkinElmer offers a range of imaging solutions that let you locate, detect, analyze and share your results your way 3D and confocal High-content imaging Quantitative pathology Pre-clinical in vivo imaging Ultraview® Opera® and Operetta® Vectra® and Lamina™ IVIS® Spectrum and Quantum CT — whether you are studying single cells in depth, screening cell populations, examining tissue sections or monitoring biological processes in whole animals. …for important new discoveries in disease research Lamina Multilabel Slide Scanner …for a clearer vision of pathology Lamina specs …for fast slide scanning Lamina Lamina is a high-throughput multilabel slide scanner that delivers the highest quality H&E, IHC and immunofluorescence whole-slide images for research pathology. With our unique technology we reduce autofluorescence and correct cross talk to give the clearest visualization of fluorescently labeled markers in FFPE tissues. More accurate results improving our understanding of disease …for a clearer vision of pathology Lamina Lamina 250 slides, highest speed and quality FL scanning Proprietary autofluorescence reduction technology inForm Tissue Finder analysis software (X2 Copies) Lamina - Best FL quality 250 slides, highest speed and quality FL scanning Lamina demo (PKI slides) RGB Unmixed composite DAPI, AF488, AF594 …autofluorescence reduction technology PerkinElmer Tissue Imaging Portfolio … inForm® Tissue Finder™ inForm® Cell Analysis™ Intelligent Image Analysis Software Lamina™ Slide scanner Nuance™ Multispectral Imaging Systems Vectra® Slide Analysis System … Capturing molecular and spatial complexity, while providing reliable quantitative results Immune Cell Phenotyping in Solid Tumors Our approach allows different phenotypes to be visualized and quantified simultaneously in the same tissue section, enabling researchers to study the relationships and distribution of these cells within tumors and the tumor microenvironment Melanoma TMA core with multiplexed immunofluorescence staining Conventional Image DAPI CD8 – Cy3 CD34 – Cy5 FoxP3 – FITC PD-L1 – coumarin Melanoma TMA core with multiplexed immunofluorescence staining Unmixed composite: DAPI – blue CD8 – green CD34 – yellow FoxP3 – purple PD-L1 – red Melanoma TMA core with multiplexed immunofluorescence staining Tissue segmentation: Tumor region – red Stroma region – green Blank – blue Melanoma TMA core with multiplexed immunofluorescence staining Per-cell quantitation Blue – CD8-/FoxP3Green – CD8+/FoxP3Red – CD8-/FoxP3+ Yellow – CD8+/FoxP3+ Melanoma TMA core with multiplexed immunofluorescence staining Compartment-specific cell counts Melanoma TMA core with multiplexed immunofluorescence staining Per-cell intensity quantitation PD-L1 0-3+ Positivity: Blue – 0+ (73.13%) Yellow – 1+ (24.06%) Orange – 2+ (2.39%) Red – 3+ (0.42%) H-Score = 30 Benefits of using multispectral imaging Multispectral imaging technology is used to acquire images at many wavelengths to better determine fluorophore/stain distribution in cells or tissue 420nm 490nm 720nm Monochrome Color (RGB) Multispectral Acquiring a series of narrow-band monochrome images leads to a spectrum of the sample at each pixel 1. Take images at different wavelengths with a CCD 5 Quantum dot vials 525, 565, 585, 605 and 655 nm 2. Assemble the data into a “cube” in memory 3. This creates a spectrum at every pixel of the CCD Spectra 650 nm 625 nm x 600 nm 575 nm 550 nm y z What is spectral unmixing? RGB image calculated from spectral image data simulated fluorescence image Nuance composite image Nuance unmixed images …showing its true colors Multiple antigens can be resolved in brightfield: a breast ER and PR example with a counterstain RGB image Nuance image BF and FL Views ER image PR image Hematoxylin image …separating the brown from the red Pushing the limits of color separation using spectral unmixing Example: Red vs. Red vs. Red Liquid Permanent Red Vector Red AEC Sample courtesy of Chris van der Loos, University of Amsterdam Bottom line: We can separate as many colors as you can reliably put into your sample …more than meets the eye inForm is user-trained to find regions of interest 1. Load images 2. Draw training regions 3. Segment image …easy does it Breast involution: Segmenting H&E-stained breast section Red: Green: Blue: Yellow: Cyan: Acini Lobular stroma Structural stroma Inflammation Fat cells …what is the percentage of inflammation? Breast involution: Segmenting H&E-stained breast section Red: Green: Blue: Yellow: Cyan: Acini Lobular stroma Structural stroma Inflammation Fat cells 19.8 % 32.3 % 38.9 % 4.9 % 10.8 % … were you close? PerkinElmer Tissue Imaging Portfolio … inForm® Tissue Finder™ inForm® Cell Analysis™ Intelligent Image Analysis Software Lamina™ Slide scanner Nuance™ Multispectral Imaging Systems Vectra® Slide Analysis System … Capturing molecular and spatial complexity, while providing reliable quantitative results Thank you for your attention! Any questions? Come visit our booth! # 7A067 ...and have a closer look at the lamina!
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