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Curriculum Vitae Svein Jarle Horn October 2014
Curriculum Vitae
Svein Jarle Horn
Department of Chemistry, Biotechnology and Food Science
Norwegian University of Life Sciences
P.O. Box 5040
N-1432 Ås
NORWAY
Phone: 47 67232488; E-mail: svein.horn@nmbu.no
Date of Birth: 28 October 1970
Citizenship: Norwegian
Education
1996-2000
1995-1996
1991-1995
PhD (Dr. ing.) in Environmental Biotechnology. Title: Bioenergy from Brown
seaweeds.
Norwegian University of Science and Technology (NTNU), Trondheim.
MSc in Biotechnology, thesis work. Title: Ammonium removal from waste water by
combining nitrification to nitrite and anaerobic ammonium oxidation.
Technical University of Delft, The Netherlands.
MSc education (siv. ing.).
The Technical University of Norway (NTH), Trondheim.
Employment
2012-present Professor in BioProcess Technology at Norwegian University Life Sciences (UMB)
2009-2012
Senior scientist in Seaweed Energy Solutions AS. Processing of seaweed to
bioethanol and biogas.
2005-2011
Senior researcher in the group of Vincent Eijsink. Research on chitin/chitinases,
cellulose disrupting proteins, enzymatic hydrolysis of barley and BSG. From 2007
mainly work with enzymatic hydrolysis of steam-exploded lignocellulose for
bioethanol production. Also involved in biogas research.
2002-2005
Post. Doc. in the group of Vincent Eijsink. Involved in research on chitinases and fish
waste hydrolysis. Norwegian University Life Sciences (UMB), Dep.of Chemistry,
Biotechnology and Food Science.
1996
Worked as process engineer at Kværner Water Systems, Sandefjord, Norway. This
included running a pilot plant for an activated sludge process (HCR).
Additional
Languages: Norwegian, Swedish, Danish, English: Fluent. German, Dutch: Basic
Awards: 1st place for “outstanding work and communication” made at the 16 th International Seaweed
Symposium held in the Philippines in 1998 (Reference [4]).
Publications (peer-reviewed journals)
[1] Jetten, M.S.M., Horn, S.J., van Loosdrecht, M.C.M., 1997, Towards a more sustainable municipal wastewater treatment
system. Water Science and Technology, 35(9), 171-180.
[2] Moen, E., Horn, S., Ostgaard, K., 1997, Biological degradation of Ascophyllum nodosum. Journal of Applied Phycology,
9(4), 347-357.
[3] Moen, E., Horn, S., Ostgaard, K., 1997, Alginate degradation during anaerobic digestion of Laminaria hyperborea stipes.
Journal of Applied Phycology, 9(2), 157-166.
Curriculum Vitae Svein Jarle Horn October 2014
[4] Horn, S.J., Moen, E., Ostgaard, K., 1999, Direct determination of alginate content in brown algae by near infra-red (NIR)
spectroscopy. Journal of Applied Phycology, 11(1), 9-13.
[5] Horn, S.J., Aasen, I.M., Ostgaard, K., 2000, Ethanol production from seaweed extract. Journal of Industrial Microbiology
& Biotechnology, 25(5), 249-254.
[6] Horn, S.J., Aasen, I.M., Ostgaard, K., 2000, Production of ethanol from mannitol by Zymobacter palmae. Journal of
Industrial Microbiology & Biotechnology, 24(1), 51-57.
[7] Horn, S.J., Ostgaard, K., 2001, Alginate lyase activity and acidogenesis during fermentation of Laminaria hyperborea.
Journal of Applied Phycology, 13(2), 143-152.
[8] Horn, S.J., Eijsink, V.G.H., 2004, A reliable reducing end assay for chito-oligosaccharides. Carbohydrate Polymers,
56(1), 35-39.
[9] Aspmo, S.I., Horn, S.J., Eijsink, V.G.H., 2005, Enzymatic hydrolysis of Atlantic cod (Gadus morhua L.) viscera. Process
Biochemistry, 40(5), 1957-1966.
[10] Hoell, I.A., Klemsdal, S.S., Vaaje-Kolstad, G., Horn, S.J., Eijsink, V.G., 2005, Overexpression and characterization of a
novel chitinase from Trichoderma atroviride strain P1. Biochim Biophys Acta, 1748(2), 180-90.
[11] Sikorski, P., Stokke, B.T., Sorbotten, A., Varum, K.M., Horn, S.J., Eijsink, V.G., 2005, Development and application of
a model for chitosan hydrolysis by a family 18 chitinase. Biopolymers, 77(5), 273-85.
[12] Sorbotten, A., Horn, S.J., Eijsink, V.G., Varum, K.M., 2005, Degradation of chitosans with chitinase B from Serratia
marcescens. Production of chito-oligosaccharides and insight into enzyme processivity. Febs J, 272(2), 538-49.
[13] Aspmo, S.I., Horn, S.J., Eijsink, V.G.H., 2005, Use of hydrolysates from Atlantic cod (Gadus morhua L.) viscera as
complex nitrogen source for lactic acid bacteria. FEMS Microbiology Letters, 248(1), 65-68.
[14] Aspmo, S.I., Horn, S.J., Eijsink, V.G.H., 2005, Hydrolysates from Atlantic cod (Gadus morhua L.) viscera as
components of microbial growth media. Process Biochemistry, 40(12), 3714-3722.
[15] Horn, S.J., Aspmo, S.I., Eijsink, V.G.H., 2005, Growth of Lactobacillus plantarum in media containing hydrolysates of
fish viscera. Journal of Applied Microbiology, 99(5), 1082-1089
[16] Vaaje-Kolstad, G., Horn, S.J., van Aalten, D.M.F., Synstad, B., Eijsink, V.G.H., 2005, The non-catalytic chitin-binding
protein CBP21 from Serratia marcescens is essential for chitin degradation. Journal of Biological Chemistry, 280 (31),
28492-28497.
[17] Horn, S.J., Sørlie, M., Vaaje-Kolstad, G., Norberg, A.L., Synstad, B., Vårum, K.M., Eijsink, V.G.H., 2006, Comparative
studies of chitinases A, B and C from Serratia marcescens. Biocatalysis and Biotransformation, 24 (1/2), 39-53.
[18] Horn, S.J., Sørbotten, A., Synstad, B., Sikorski, P., Sørlie, M., Vårum, K.M., Eijsink, V.G.H., 2006, Endo/exo
mechanism and processivity of three family 18 chitinases that make up the chitinolytic machinery of Serratia marcescens.
Febs Journal, 273, 491-503.
[19] Sikorski, P., Sørbotten, A., Horn, S.J., Eijsink, V.G.H., Vårum, K.M, 2006, Serratia marcescens chitinases with “tunnelshaped” substrate-binding grooves show endo-activity and different degrees of processivity during enzymatic hydrolysis of
chitosan, Biochemistry, 45, 9566-9574.
[20] Horn, S.J., Sikorski, P, Cederkvist, J.B., Vaaje-Kolstad, G., Sørlie, M., Synstad, B., Vriend, G., Vårum, K.M., Eijsink,
V.G.H., 2006, Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides. PNAS, 103 (48),
18089-18094.
[21] Horn, S.J., Aspmo, S.I., Eijsink, V.G.H., 2007, Evaluation of different cod viscera fractions and their seasonal variation
used in a growth medium for lactic acid bacteria. Enzyme and Microbial Technology, 40, 1328-1334.
[22] Krokeide, I.M., Synstad, B., Gåseidnes, S., Horn, S.J., Eijsink, V.G.H., Sørlie, M., 2007, Natural Substrate Assay for
Chitinases Using High Performance Liquid Chromatography: A Comparison with Existing Assays. Analytical biochemistry,
363, 128-134.
[23] Synstad, B., Vaaje-Kolstad, G., Cederkvist, F.H., Saua, S.F., Horn, S.J, Eijsink, V.G.H., Sørlie, M., 2008, Natural
Expression and characterization of endochitinase C from Serratia marcescens BJL200 and its purification by a one-step
general chitinase purification method. Bioscience, Biotechnology, and Biochemistry. 72 (3), 715-723.
[24] Treimo, J.R., Eijsink, V.G.H., Horn, S.J., 2008, Enzymatic solubilization of proteins in brewer’s spent grain. J. Agric.
Food Chem,.56 (13), 5359-5365.
Curriculum Vitae Svein Jarle Horn October 2014
[25] Eijsink, V.G.H., Vaaje-Kolstad, G., Vårum, K. M., Horn, S.J., 2008, Towards new enzymes for biofuels: lessons from
chitinase research . Trends in Biotechnology, 26 (5), 228-235.
[26] Treimo, J., Westereng, B., Horn, S.J., Forssell, P., Robertson, J., Faulds, C., Waldron, K., Buchert, J., Eijsink, V.G.H.,
2009, Enzymatic solubilization of brewers’ spent grain by combined action of carbohydrases and peptidases. J. Agric. Food
Chem., 57, 3316-3324.
[27] Zakariassen, H., Aam, B.B., Horn, S.J., Vårum, K.M., Sørlie, M., Eijsink, V.G.H., 2009, Aromatic residues in the
catalytic center of Chitinase A from Serratia marcescens affect processivity, enzyme activity and biomass-converting
efficiency. Journal of Biological Chemistry, 284, 10610-10617.
[28] Horn, S.J., Eijsink, V.G.H., 2010, Enzymatic hydrolysis of steam exploded hardwood using short processing times.
Bioscience, Biotechnology, and Biochemistry, 74 (6), 1157-1163.
[29] Vaaje-Kolstad, G., Westereng, B., Horn, S.J., Liu, Z., Zhai, H., Sørlie, M., and Eijsink, V.G.H., 2010, An Oxidative
Enzyme Boosting the Enzymatic Conversion of Recalcitrant Polysaccharides. Science, 330 (6001), 219-222.
[30] Horn, S.J., Nguyen, Q.D., Westereng, B., Nilsen, P., Eijsink, V.G.H., 2011, Screening of steam explosion of wheat straw
for enzymatic hydrolysis and ethanol fermentation, Biomass and Bioenergy, 35 (12), 4879-4886.
[31] Blomqvist, J., South, E., Tiukova, L., Haddad, Momeni, M.H., Hansson, H., Ståhlberg, J., Horn, S.J., Schnürer, J.,
Passoth, V., 2011, Fermentation of lignocellulosic hydrolysate by the alternative industrial ethanol yeast Dekkera
bruxellensis, Letters in Applied Microbiology, 53 (1), 73-78.
[32] Horn, S.J., Estevez. M.M., Nielsen, H.K., Linjordet, R., Eijsink, V.G.H., 2011, Biogas production and saccharification
of Salix pretreated at different steam explosion conditions, Bioresource Technology, 102 (17), 7932-7936.
[33] Forsberg, Z., Vaaje-Kolstad, G., Westereng, B., Bunæs, A.C., Stenstrøm, Y., MacKenzie, A., Sørlie, M., Horn, S.J.,
Eijsink, V.G.H., 2011, Cleavage of cellulose by a CBM33 protein, Protein Science, 20 (9), 1479-1483.
[34] Westereng, B, Ishida, T, Vaaje-Kolstad, G, Wu, M., Eijsink, V.G.H., Igarashi, K, Samejima, M., Stålberg, J., Horn, S.J.,
Sandgren , M. 2011, The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent
oxidative enzyme that cleaves cellulose, PLoS ONE, 6(11): e27807.
[35] Vivekanand, V., Eijsink, V.G.H., Horn, S.J., 2012, Biogas production from the brown seaweed Saccharina latissima:
thermal pretreatment and co-digestion with wheat straw, Journal of Applied Phycology, 24 (5), 1295-1301.
[36] Schütt, F., Westereng, B., Horn, S.J., Puls, J., Saake, B., 2012, Steam refining as an alternative to steam explosion,
Bioresource Technology, 111, 476-481.
[37] Horn, S.J., Sørlie, M., Vårum, K.M., Väljamäe, P., Eijsink, V.G.H., 2012, Measuring processivity, Methods in
Enzymology, 510, 69-96.
[38] Horn, S.J., Vaaje-Kolstad, G., Westereng, B., Eijsink, V.G.H., 2012, Novel enzymes for the degradation of cellulose,
Biotechnology for Biofuels, 5, 45.
[39] Vivekanand,V., Ryden,P., Horn, S.J., Tapp, H.S., Wellnerd, N., Eijsink, V.G.H., Waldron, K.W., 2012, Impact of steam
explosion on biogas production from rape straw in relation to changes in chemical composition, Bioresource Technology, 123,
608-615.
[40] Payne, C.M., Baban, J., Horn, S.J., Backe, P.H., Arvai, A.S., Dalhus, B., Bjørås, M., Eijsink, V.G.H., Sørlie, M.,
Beckham, G.T., Vaaje-Kolstad, G., 2012, Hallmarks of processivity in glycoside hydrolases from crystallographic and
computational studies of the Serratia marcescens chitinases, Journal of Biological Chemistry, 287, 36322-36330.
[41] Westereng, B., Agger, J.W., Horn, S.J., Vaaje-Kolstad, G., Aachmann, F.L., Stenstrøm, Y.H., Eijsink, V.G.H., 2013,
Efficient separation of oxidized cello-oligosaccharides generated by cellulose degrading lytic polysaccharide
monooxygenases, Journal of Chromatography A, 1271, 144-152.
[42] Pope, P.B., Vivekanand, V., Eijsink, V.G.H., Horn, S.J., 2013, Microbial Community Structure in a Biogas Digester
Utilizing the Marine Energy Crop Saccharina latissima, 3 Biotech, 3, 407-414.
[43] Vivekanand, V., Olsen, E.F., Eijsink, V.G.H., Horn, S.J., 2013, Effect of different steam explosion conditions on
methane potential and enzymatic saccharification of birch, Bioresource Technology, 127, 343-349.
[44] Wu, M., Beckham, G.T., Larsson, A.M., Ishida, T., Kim, S., Crowley, M.F., Payne, C.M., Horn, S.J., Westereng, B.,
Ståhlberg, J., Eijsink, V.G.H., Sandgren, M., 2013, Structural and computational characterization of the lytic polysaccharide
Curriculum Vitae Svein Jarle Horn October 2014
monooxygenase GH61D from the basidiomycota fungus Phanerochaete chrysosporium, Journal of Biological Chemistry,
288, 12828-12839.
[45] Vaaje-Kolstad, G., Horn, S.J., Sørlie, M., Eijsink, V.G.H., 2013,The chitinolytic machinery of Serratia marcescens – a
model system for enzymatic degradation of recalcitrant polysaccharides, FEBS Journal, 280, 3028-3049.
[46] Risberg, K., Suna, L., Levén, L., Horn, S.J., Schnürer, A., 2013, Biogas production from wheat straw and manure –
impact of pretreatment and process operating parameters. Bioresource Technology,149, 232-237.
[47] Tiukova, I., de Barros Pita, W., Sundell, D., Haddad momeni, M., Horn, S.J., Ståhlberg, J., de Morais Jr., M.A., Passoth,
V., 2014, Adaptation of Dekkera bruxellensis to lignocellulose-based substrate. Biotechnol Appl Biochem, 61 (1), 51-57.
[48] Agger, J. W., Eijsink, V.G.H., Horn, S.J., 2014, On the determination of water content in biomass. Bioenerg. Res., 7,
442-449.
[49] Isaksen, T., Westereng, B., Aachmann, F.L., Agger, J.W., Ludwig, R., Haltrich, D., Eijsink, V.G.H., Horn, S.J., 2014, A
C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides, 289, 2632-2642.
[50] Ryden, P., Gautier, A., Wellner, N., Tapp, H.S., Horn, S.J., Eijsink, V.G.H., Waldron, K.W., 2014, Changes in the
composition of the main polysaccharide groups of oil seed rape straw following steam explosion and saccharification.
Biomass and Bioenergy, 61, 121-130.
[51] Vivekanand, V., Olsen, E.F., Eijsink, V.G.H., Horn, S.J., 2014, Methane potential and enzymatic sacchariﬁcation of
steam exploded bagasse. BioResources, 9(1), 1311-1324.
[52] Agger, J.W., Isaksen, T., Várnai, A., Vidal-Melgosa, S., Willats, W.G.T., Ludwig, R., Horn, S.J., Eijsink, V.G.H.,
Westereng, B., 2014, Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant
cell wall degradation. PNAS, 111(17), 6287-6292.
[53] Estevez. M.M., Linjordet, R., Horn, S.J., Morken, J., 2014, Improving nutrient fixation and dry matter content of an
ammonium-rich anaerobic digestion effluent by struvite formation and clay adsorption. Water Science and Technology,
70(2):337-344.
[54] Bauer, A., Lizasoain, J., Theuretzbacher, F., Agger, J.W., Rincón, M., Menardo, S., Saylor, M.K., Enguidanos, R.,
Nilsen, P.J., Gronauer, A., Horn, S.J., 2014, Steam explosion pretreatment for enhancing biogas production of late harvested
hay. Bioresource Technology, 166, 403-410.
[55] Hagen, L.H., Vivekanand, V., Linjordet, R., Pope, P.B., Eijsink, V.G.H., Horn, S.J., 2014, Microbial community
structure and dynamics during co-digestion of whey and cow manure in CSTR systems. Bioresource Technology, 171: 350359.
[56] Solli, L., Håvelsrud, O.E., Horn, S.J., Rike, A.G., 2014, Metagenomic characterization of the microbial communities in
parallel biogas reactors co-digesting fish ensilage and manure. Biotechnology for Biofuels, in press.
[57] Liu, Z., Penman, G., Gay, L.M., Westereng, B., Mathiesen, G., Horn, S.J., Vaaje-Kolstad, G., Van Aalten, D.M.F.,
Eijsink, V.G.H., 2014, Structure and function of a broad-specificity chitin deacetylase from Aspergillus nidulans FGSC A4.
Manuscript.
[58] Treimo, J., Westereng, B., Horn, S.J., Forssell, P., Robertson, J., Faulds, C., Waldron, K., Buchert, J., Eijsink, V.G.H.,
2014, Comparison of enzymatic hydrolyzability of proteins and polysaccharides in brewers` spent grain from different
European breweries. Manuscript.
Books/Book Chapters
Horn, S.J., 2009, Seaweed Biofuels: Production of Biogas and Bioethanol from Brown Macroalgae. VDM Verlag. ISBN 9783-639-15307-1.
Horn, S.J., 2013, A tetra transition away from fossil fuels. In: The changing environment for human security: Transformative
Approaches to Research, Policy, and Action. Eds. O’Brien, K., Sygna, L. and Wolf, J. London: Routledge-Earthscan, 392401.