1. No category

Food & Feed Analysis
Juni/2007
II/2014
RIDASCREEN® Bacitracin Art. No. R2901
Bacitracin belongs to the group of
polypeptide antibiotics. Through
inhibition of cell wall synthesis, it has a
broad spectrum bactericidal effect
against both gram-positive and
gram-negative bacteria. In addition to
veterinary application, bacitracin can be
used as antimicrobial growth promoter
in animal husbandry. Thereby, residues
can remain in foodstuff of animal origin
and may pose a health risk to
consumers. With the new competitive
RIDASCREEN® Bacitracin test for
quantitative determination of bacitracin
in milk, meat, eggs, feed and urine the
residues of this antibiotic can be
documented. As the maximum residue
limits of the European Union and the
Russian Customs Union are within the
standard range and the detection limits
are below these regulatory limits, the
test kit allows a sensitive and simple
analysis at the same time. The test has
no significant cross reactivity to other
antibiotics and is therefore highly
specific for bacitracin.
RIDASCREEN® Bacitracin Art. No. R2901
RIDA® Tetracyclin Spiking Solution
Art. No. R3599
R-Biopharm offers a new tetracyclin
spiking solution which can be used for
validation and fortification experiments
in conjunction with the enzyme
immunoassay RIDASCREEN® Tetracyclin
(Art. No. R3505). The spiking solution
kit contains a tetracycline lyophilisate
and a buffer for reconstitution. The
lyophilisate produces a 10 ml stock of
spiking solution with a concentration
of 100 ng/ml. The RIDA® Tetracyclin
Spiking Solution has a shelf life of
12 months from production date and
RIDA® Tetracyclin Spiking Solution
Art. No. R3599
the stock solution can be stored up to
6 months at 4 °C after reconstitution.
New products
Processed assay controls for gluten analysis available:
Set of 3 processed Gliadin Assay Controls Art. Nr. R7012
R-Biopharm offers new assay controls for
the review of extraction with Cocktail
(patented). Until now this review was
only possible for the extraction with
ethanol, provided by the
unprocessed assay controls (Art.
No. R7010 – Set of 3 Gliadin Assay
Controls).
Now the analysis of highly heated
(processed) food with the official
Codex Alimentarius method (Art. No.
R7001 – RIDASCREEN® Gliadin + Cocktail
(patented)) can be monitored from the
extraction over ELISA procedure and result
evaluation. The set of 3 processed gliadin
assay controls (Art. No. R7012) consists of
an exactly defined highly heated snack,
which was produced for an AACCI
collaborative trial and therefore it is also
very well characterized. The material was
newly mixed and will be provided in three
different concentrations (5.5 ppm, 20 ppm
and 50 ppm prolamin) in this set. These
assay controls provide the possibility to
establish a quality control card for the
review of the whole analytic process.
Enzytec™ Glucose Remover Art. No. E3400
The new Glucose Remover kit is especially
helpful when testing lactose free
samples in conjunction with the
Lactose/D-Glucose kit (Art. No.
10986119035). In Germany, this
combination is currently being
investigated to become
mandatory by law. In addition to
this, the Glucose Remover kit can
be used in every case where a glucose
excess must be eliminated. This can be
the case when using for example the
Sucrose/ D-Glucose (Art. No. 10139041035)
or the Sucrose/D-Glucose/D-Fructose
(Art. No. 10716260035) assays for the
determination of sucrose in honey. The
procedure can also be applied for the
Maltose/Sucrose/ D-Glucose (Art. No.
11113950035) and the Starch assays
(Art. No. 10207748035). All the tests
mentioned above do not work properly
when the sample contains a large excess
of glucose, because the difference between
the two cuvettes will be low and not
reproducible (Δ A < 0.100). For this
reason, the instructions for use of these
assays include a special procedure to
remove the glucose excess in the sample,
by using the enzymes glucose oxidase
(GOD) and catalase.
The Enzytec™ Glucose Remover (Art. No.
E3400) contains the reagents that are
needed to apply this procedure on
32 samples.
The control sample is a liquid solution. It
contains 25 g/l glucose and 1 g/l lactose
in order to simulate the concentrations in
lactose free milk. It also contains 1 g/l
sucrose in order to simulate a honey
sample with low sucrose and high glucose
concentrations. The control sample is
handled like a normal sample where the
glucose excess must be removed via the
Glucose Remover procedure.
For detailed information on the Glucose
Remover kit, instructions for use are
available from your local distributor.
Our products
How to ensure gluten-free beer?
As summer is coming closer, the days
become longer and people stay out to
enjoy the extra hours of sun. Who would
not like to have a fresh drafted and chilled
beer in hand?
page 2
For people with celiac disease that
imagination is nearly impossible, because
gluten, a molecule in high quantity in beer
can in this case cause intestinal
inflammation. There are just a few beers
made from different gluten free cereals or
enzymatically treated beers that are an
alternative.
But, how can one guarantee that these
beers are gluten free? For the analysis of
gluten in beer different associations like
MEBAK (Middle European Brewingtechnological Analysis Commission), the
AOECS (Association of European Coeliac
Societies), the ASBC (American Society of
Brewing Chemists) and the AACCI
(American Association of Cereal Chemists
International) recommend the
RIDASCREEN® Gliadin competitive (Art. No.
R7021), because only this ELISA is able to
analyze gluten in beer.
Gluten proteins in beer are fragmented to
peptides due to the brewing process.
These peptides cannot be analyzed with a
common sandwich ELISA, because the
fragments are too small. An analysis of a
supposed gluten free beer with a sandwich
ELISA may lead to a non-detection of toxic
gluten peptides, which may exist in the
analyzed beer. The RIDASCREEN® Gliadin
competitive (Art. No. R7021) is based on a
competitive principle, therefore an analysis
of small fragments is possible. Further
advantages is the calibrator (standard) row,
which consists of fragmented gluten and
therefore mirrors the situation in beer, and
the use of the R5 antibody recommended
by the CODEX Alimentarius as type I
method. For these reasons the
RIDASCREEN® Gliadin competitive is
especially suitable to analyze gluten in
beer.
Recently in a collaborative study of AACCI
it was shown that the RIDASCREEN® Gliadin
competitive is a reliable method for the
analysis of gluten in fermented or
hydrolyzed food or beverages.
SureFood® PCR products from our partner
CONGEN Biotechnologie GmbH, Berlin
News
Due to organisational changes, the
previous real-time PCR kits for pathogens
and microorganisms will be transferred to
a new product category, namely SureFast®,
and will obtain new order numbers
(F instead of S). The composition and
properties of the kits remain unchanged.
For other real-time PCR parameters
(allergens, GMO and animal specification),
the well-known brand name SureFood®
that was introduced over a decade ago will
remain unchanged.
Name of the assay
SureFast® PREP
Campylobacter
SureFast® PREP Listeria
SureFast® PREP E. coli
SureFast® PREP Bacteria I
SureFast® PREP Aqua
SureFast® PREP
DNA/RNA Virus
SureCycle - Kit
SureCC Color
Compensation Kit I
Color Compensation Kit
for Light Cycler 480 II
SureCC Color
Compensation Kit II
Color Compensation Kit
for Light Cycler LC 2.0
SureFast®
Inhibition Control
SureFast®
E. coli eae gene PLUS
SureFast®
STEC Screening PLUS
SureFast®
Clostridium botulinum
Screening PLUS
SureFast®
Campylobacter PLUS
SureFast® Listeria
monocytogenes PLUS
Name of the assay
SureFast®
Cronobacter PLUS
SureFast®
Staphylococcus aureus
PLUS
SureFast®
Listeria Screening PLUS
SureFast®
Vibrio Screening PLUS
SureFast®
Vibrio cholerae PLUS
SureFast® Vibrio
parahaemolyticus PLUS
SureFast®
Vibrio vulnificus PLUS
SureFast® Clostridium
perfringens PLUS
SureFast®
Bacillus cereus group
PLUS
SureFast® Legionella
pneumophila PLUS
SureFast®
Legionella Screen PLUS
SureFast®
Norovirus PLUS
SureFast® Norovirus/
Hepatitis A 3plex
Old Art. No.
S5114
New Art. No.
F5114
S5116
F5116
S5117
F5117
S5118
F5118
S5119
F5119
S5120
F5120
S5121
F5121
S5123
F5123
S5126
F5126
S5501
F5501
S5502
F5502
S7001
F7001
S7124
F7124
SureFast®
Hepatitis A PLUS
S7125
F7125
page 3
Old Art. No.
S1008
New Art. No.
F1008
S1009
S1013
S1021
S1023
S1051
F1009
F1013
F1021
F1023
F1051
S4001
S4009
F4001
F4009
S4010
F4010
S4050
F4050
S5104
F5104
S5105
F5105
S5110
F5110
S5112
F5112
S5113
F5113
News from our cooperation partner GEN-IAL
New: Proof of 27 beer spoiling bacteria in just one reaction
Real-time PCR multiplex panel for modern beer analysis
First-Beer differentiation PCR Kit
Art. No. TPBD 0096
The real-time PCR kits from GEN-IAL GmbH
have been tried and tested as group-specific screening kits and individual speciesspecific kits for over a decade. In addition
to the advantageous aspect of the open
platform – the kits can be used with most
of the commercially available qPCR devices
and, in turn, are open to other uses – the
group wide detection offers the advantage
of increasingly recognising new, adaptable
beer spoiling bacteria such as the current
Lactobacillus parabrevis, therefore making
new validations unnecessary.
After consultation with several breweries, a
new test was developed that, according to
current increased requirements, enables
screening and detection of the most
important beer spoiling bacteria in just one
analysis.
Pre-coated 8-fold PCR strips each with
3 different parameters can be used on the
96-well block cycler format. The operator
uses only 2.5 µl from the sample DNA,
inserts this into each of the 8 cavities of
one of the strips and adds 17.5 µl of
premix. Subsequently, the measurement
process can be immediately started. As a
consequence, in the 3 detection channels
FAM, VIC/HEX and Cy5, a total of
27 parameters are recorded per sample/8th
strip (see table). This enables the user to
directly identify a variety of beer spoiling
bacteria or rather complete relevant
groups, with limited handling. In addition
to the beer spoiling bacteria, a signal for
Enterbacteriaceae would refer to external
pollution. 3-channel thermo cyclers such
as the Roche LightCycler®480,
Bio-Rad CFX96, ABI 7500, or Agilent
Mx3005P are required. The openness of
this concept enables the user not to have
to commit to one system and, furthermore,
is easily expandable.
Well
FAM
HEX
Cy5
1
L. brevis/brevisimilis/parabrevis
L. lindneri
L. casei/paracasei
2
L. buchneri/parabuchneri
L. collinoides/paracollinoides
L. perolens/L. harbinensis
3
L. backii
L. rossiae
L. acetotolerans
4
L. plantarum/paraplantarum
L. coryniformis
Internal Amplification Control
5
Pediococcus spp.
Ped. damnosus
Ped. claussenii
6
Pectinatus spp.
Megasphaera spp.
Positive Control
7
S. diastaticus
Pichia anomala
Dekkera spp.
8
Enterobacteriaceae
If you are interested in our products,
please contact your local distributor.
RIDA®SOFT Win / RIDA®SOFT Win.net Art. No. Z9996
Current
version:
1.87
page 4
If you are interested in an update of RIDA®SOFT Win or
RIDA®SOFT Win.net, please contact your local distributor.
Information from R-Biopharm Rhône,
Scotland
EFSA assess EU limits for deoxynivalenol (DON) in cereals
EFSA has recently delivered an opinion on
the risks to public health related to a
possible increase of the maximum level for
deoxynivalenol (DON) in certain semiprocessed cereal products from 750 μg/kg
to 1000 μg/kg. Based on modeling
different scenarios, it was concluded that
an increase of the DON maximum level
would increase exposure and consequently
exceed the group health based guidance
values for this mycotoxin. The EFSA Panel
did however see a need for more
occurrence data on DON acetyl-derivatives,
using sufficiently sensitive methods in
order to assess their impact on the health
risk associated with an increase of the
maximum level for DON.
http://www.efsa.europa.eu/en/efsajournal/
pub/3490.htm
R-Biopharm DONPREP® immunoaffinity
columns offer highly specific clean-up for
both DON and 3-acetyl DON for use with
LC and LC-MS. The complementary DZT
MS-PREP® column offers clean-up to those
laboratories wanting to monitor not only
DON, but also zearalenone and T-2 / HT-2
toxins fusarium toxins. These columns are
widely used in food control enabling
official laboratories to meet method
performance requirements set out in EU
Regulations.
R-BiopharmAG
Analysis of gluten-free beer
Determination of gliadin/gluten in hydrolyzed food
with the R5 antibody
RIDASCREEN® Gliadin competitive
• AACCI38.55.01
• TTBRulingNumber2012-2forbeer
• AOECSStandardR5ELISAforhydrolyzedfood
• MEBAKMethod2.6.5beeranalysis
Related products
•ELISA:RIDASCREEN®Gliadin•RIDASCREEN®FASTGliadin•LateralFlow:RIDA®QUICKGliadin
•PCR:SureFood®ALLERGENGluten
page 5
Fairs and conferences Representative: R-Biopharm AG
07.09. - 10.09.2014
AOAC Annual Meeting
Florida, USA
05.10. - 08.10.2014
AACC International Annual Meeting
Rhode Island, USA
29.10. - 30.10.2014
Food Analysis Congress
Barcelona, Spain
Allergen Workshop (LFD, ELISA, PCR) 10/2014
Date:
14.10. -15.10.2014
(optional: intensive qPCR lab training on 16.10.)
Time:
14.10.: 10 am - 5 pm / 15.10: 9 am - 5 pm
(optional 16.10: 9 am - 2 pm)
Location:
R-Biopharm AG, Darmstadt, Germany
Program:
The workshop covers practical work in the lab and presentations
by allergen experts. The most used technologies for allergen
testing such as LFD (lateral flow testing), ELISA and qPCR will be
discussed.
The number of participants is limited to 30.
Optionally, it is possible to register for an allergen qPCR intensive
training on 16.10. (limited to 6 participants).
The complete Allergen Workshop will be in English language.
Participation Fee:
14.10. - 15.10. 700.00 EUR + 19 % VAT and additionally for the
16.10. 250.00 EUR + 19 % VAT
(included: coffee breaks, lunch, hand-outs, material for practical
demonstrations, participation certificate, soiree (evening
entertainment), 1 overnight stay in the RAMADA Hotel
(14.10. - 15.10.), (+ 1 overnight stay for participants of qPCR
intensive training (15.10. - 16.10)).
At the end of the training all participants will receive a voucher
for one allergen test kit (free choice).
Information and
Registration:
Please contact Jasmin Wolf
Phone: +49 (0) 61 51 - 81 02-782 or
E-mail: j.wolf@r-biopharm.de
Vitamin Workshop (HPLC, VitaFast®, ELISA) 10/2014
Date:
28.10. - 30.10.2014
Time:
28.10.: 9:00 - 16.30 h / 29.10.: 9:00 - 16:30 h / 30.10: 9:00 - 15:00 h
Location:
R-Biopharm Rhône Ltd., Block 10 Todd Campus,
West of Scotland Science Park, Acre Road, Glasgow, Scotland
Participation Fee:
550.00 EUR + 19 % VAT
(training includes coffee breaks, lunch, hand-outs, material for
practical demonstrations, participation certificate,
soiree (evening entertainment, first two evenings).
3 overnight stays in the Carlton George Hotel ****)
Information:
R-Biopharm Rhône Ltd. – Marketing department
Phone: +44 (0) 141 - 945-2925 or
E-mail: info@r-biopharmrhone.com
The next R-Biopharmnews will be published in the IIIrd quarter 2014.
R-Biopharmnews is edited by
R-Biopharm AG
An der neuen Bergstraße 17
64297 Darmstadt, Germany
Reg.-Nr.: Amtsgericht Darmstadt, HRB 8321
Phone: +49 (0) 61 51 - 81 02-0
Fax: +49 (0) 61 51 - 81 02-40
E-mail: info@r-biopharm.de
www.r-biopharm.com