1. science
2. physics

About Ato ID
Ato ID is a manufacturer of disposable SERS substrates ‘Randa’ and ‘Mato’. Company addresses the
challenges of affordable and simplified diagnostics as well as threats for safe environment by introducing SERS
innovations. Latest developments in SERS (Surface Enhanced Raman Scattering) substrates have expanded our range
of advantageous solutions for Raman spectroscopy users.
Being 8 times more sensitive than the current gold standard on the market, patented sensors feature competitive
price, scalable production and good repeatability. Comparing to other analytical techniques, the biggest advantages
of SERS technique lay in single and simultaneous analysis for multiple targets and elimination of expensive reagents
or time-consuming sample preparation steps associated with other techniques.
Composition of the SERS substrates
range of excitation wavelengths like golden ones,
The active SERS area is formed using an ultra-short
pulse laser on a soda-lime glass substrate. The substrate
material is a weak Raman scattered and therefore
particularly suitable for SERS (as compared to most
crystalline materials). The resulting surface structure
features stochastic nanopattern, which meets good
resonance characteristics for various excitation
wavelengths and adsorbed analyte molecules. A single
SERS substrate can be used for various experimental
conditions, analytes and results in a very high relative
enhancement ratio of the Raman scattering up to 106.
Silver-plated SERS works well not only in the IR
but also in a visible (blue, green) range as well.
Convenient size of the overall substrate (25 x 25
x 1 mm) is approximately a third part of a regular
microscope slide, therefore it fits nicely into universal
multi-wavelength Raman microscopes and can be used
with dedicated compact SERS readers or spectrometers
as well. Active area of the standard 'Randa' and 'Mato'
SERS substrates is 4 x 4 millimetres by default.
Flexible manufacturing technique allows to change
(increase or decrease) the size of the SERS area on
request. Active areas as large as 10 x 10 mm have been
produced specifically for application of electrodes
(electro-chemical experiments).
All 'Randa' and 'Mato' Raman substrates are
vacuum packed in a cleanroom environment. No glue
or other chemical substances are used during
manufacturing, i.e. for attachment of active area chips
to submounts (which is a common feature of
counterpart producs.
Product specifications
Size (mm)
25 x 25 x 1
Active area (mm)
4x4
Sampling method
Drop deposition, immersion
Recommended excitation wavelength
Randa - from 445 nm to NIR
Mato - from 600 nm to NIR
About Raman spectroscopy
Raman spectroscopy is one of the most flexible and accurate technologies for molecular diagnostics. The only
drawback - usually low intensity of Raman scattering signal - is eliminated by using a plasmonic SERS substrate,
sputter-coated with silver (or gold), which enhances the Raman spectra significantly.
Contacts
„Ato ID“, UAB
Email: info@atoid.com
Phone nr: +370 616 83149
Website: www.atoid.com
Adress: Kalvariju str. 125, 08221 Vilnius
Adenine analysis on `Randa` substrate
Overview
The `Randa` SERS substrate is made of laser nanopatterned soda-lime glass, coated with silver. The
active area is typically 4 by 4 mm and overall substrate
dimensions are 25x25x1 mm.
Experimental data
About the analyte: adenine is one of the two purine
the presence of various diseases. Quantitation of
nucleobases (the other being guanine) used in forming
nucleotides of the nucleic acids.The shape of adenine
is complementary to either thyine in DNA or uracil in
RNA. Adenine plays a significant role in biological
system as it has wide spread effect to coronary and
cerebral circulation, energy transduction, enzymatic
reactions as cofactors, and even in cell signaling.
Abnormal changes of its concentration may indicate
adenine is, therefore, critically needed for the
studies of a wide variety of biological issues.
Sampling method: immersion; SERS substrates we
immeresed into water solutions of Adenine and rinsed
with deionized water afterwards.
Raman system: Renishaw InVia; Laser: 785 nm, 1
mW power; Lens: 5 x magnification;
Below we provide experimental data, which is
used to evaluate enhancement repeatability of
‘Randa’ substrates.
immersion; b) after 2 hours of immersion; c) after 1 hour
of immersion.
Fig. 2. 0,5 mM adenine SERS spectra. a) after 24 hours of
immersion; b) after 2 hours of immersion; c) after 1 hour
of immersion.
Fig. 3.
Adenine SERS spectra intensity dependence on time.
Analysis of antibiotics on „Randa“ substrates
Overview: The `Randa` SERS substrate is made of laser
nano-patterned soda-lime glass, coated with silver. The
active area is typically 4 by 4 mm and overall substrate
dimensions are 25x25x1 mm.
Experimental data
About analytes: In this study we investigated the
ability of Raman spectroscopy to differentiate between
various antibiotics that are in use for cattle treatment.
Excess of those chemicals are often found in milk and
other dairy products. In figures below, you can find the
Raman spectra from pure materials and SERS spectra
from low concentrations antibiotics solutions.
Sampling method: immersion; SERS substrates were
immersed into antibiotics solutions and rinsed with
deionized water afterwards.
Raman system: Jobin Yvon;
Laser: 633 nm, 1 mW power ; Lens: 10 x
magnification;
Experimental data provided below.
Genta
Penstrep
500
250
Raman
SERS
Raman
SERS
100
300
1000
Scattering intensity
1458
1274
1166
1000
974
Scattering intensity
150
1600
400
200
200
100
50
0
0
200
400
600
800
1000
1200
Raman shift, cm
1400
1600
1800
-1
Fig. 1. Antibiotic „Penstrep“ Raman and SERS spectra.
Active compuonds - penicillin G (sodium salt) and
streptomycin sulfate.
200
400
600
800
1000
1200
1400
1600
1800
Raman shift, cm-1
Fig. 2. Antibiotic „Genta“ Raman and SERS spectra.
Active compound – gentamycin sulfate.
Zepilen
Tetroxy
SERS
500
Raman
SERS
300
1451
1533
1578
400
1278
1321
Scattering intensity
400
200
736
1345
1090
778
666
200
478
Scattering intensity
600
100
0
0
200
400
600
800
1000
1200
1400
1600
1800
Raman shift, cm-1
Fig. 3. Antibiotic „Zepilen“ Raman and SERS spectra.
Active compound cephazolin.
200
400
600
800
1000
1200
1400
1600
1800
Raman shift, cm-1
Fig. 4. Antibiotic „Tetroxy“ SERS spectra. Active
compound oxytetrac