Productand Pricelist Stemgent 2015 Tissue Dissociation ESC/iPSC Cells, Media & Reprogramming Tools 3D Cell Culture Technology & Cell Culture Models Recombinant Proteins, Cytokines, Biomatrix Proteins & Elisa Cell Based Assays & Protein Labeling for Microscopy, FACS and HTS Cell Culture Tools www.pelobiotech.com Stemgent Products 2015 Pelobiotech offers you a broad range of products for ES/iPS Cells from all members of the Reprocell Family [ReproCell – Stemgent – Reinnervate – BioServe]. Stemgent, Inc. is developing innovative technology and application solutions for the advancement of stem cell research. Stemgent products are designed by leading stem cell researchers worldwide. Reprogramming Solutions from Stemgent Through Dr. Shinya Yamanaka’s pioneering work on the reprogramming of somatic cells to a pluripotent-like state the iPS cell has become an invaluable tool for strategies in regenerative medicine, disease modeling, drug discovery, and basic research in cell development. For a whole range of reprogramming methods, Stemgent provides the quality, knowledge, and expertise you depend on. Fig. 1:©Stemgent Faster, virus-free reprogramming mRNA Reprogramming microRNA-Enhanced mRNA reprogramming – even faster mRNA Reprogramming - Generate colonies in as early as 16 days The optimized Stemgent mRNA Reprogramming System enables non-viral, non-integrating, clinically-relevant reprogramming of human cells in less than 20 days with an expansion of new lines in culture in as little as 3 weeks. This is a big step forward, compared to lentivirus, Sendai virus, and other methods that can take over 20 weeks to confirm viral remnants no longer remain. Six major steps are required for mRNA reprogramming along with morphology changes of human iPS cells (as shown in timeline below). PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Fig. 2:Phase-contrast images taken on Day 1-12. Phase-contrast image and fluorescent staining of TRA-1-60 taken on Day 14 (© Stemgent, Inc.).. microRNA-Enhanced mRNA reprogramming - Generate colonies in as early as 12 days, faster reprogramming kinetics & higher efficiency microRNAs play an important role in the maintenance and retention of pluripotency for both ES cells and iPS cells, as ongoing research has demonstrated 1,2. These endogenous, small, non-coding RNAs target and down-regulate mRNAs by blocking gene expression via translational repression or degradation of functionally transcribed mRNAs. In 2012, two groups succeeded in functionally reprogramming human fibroblasts to a pluripotent state in the absence of exogenous reprogramming factor expression, but with the help of specific microRNA combinations 3, 4, 5. microRNAs induce self-renewal and proliferative networks, inhibit TLR-induced inflammatory cytokine expression, and promote a mesenchymal to epithelial (MET) transition, which is particularly advantageous when working with difficult to reprogram patient samples 6, 7. Stemgent developed a new microRNA-enhanced mRNA Reprogramming method, which combines the use of the Stemgent mRNA Reprogramming Kit, the Stemgent microRNA Booster Kit, and the StemfectTM RNA Transfection Kit, to generate high-quality human iPS cell lines. These modifications to the traditional feeder-based mRNA reprogramming protocol results in faster reprogramming kinetics (see timeline Fig. 3), simpler experimental set up simpler, allows for reprogramming more than twice the number of wells (9 wells in 6-well format) and shows higher efficiency. This method is recommended for achieving the best results from your reprogramming experiment when compared to other available reprogramming methods. Fig. 3: microRNA enhanced mRNA Reprogramming Timeline. 2 microRNA transfections and 11 mRNA transfections in total are required for this system. By Day 13, emerging iPS cell colonies are often able to be identified by morphology and life staining. But depending on the target cell type, cell colonies may be ready to be picked and replated earlier or later than shown in the timeline (© Stemgent, Inc..). PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Fig. 4: Morphological progression comparison of diseased patient fibroblasts during reprogramming with and without microRNA: The above panel demonstrates representative morphological progression of adult diseased patient fibroblasts with and without the Stemgent-Asterand microRNA booster cocktail (© Stemgent, Inc..). 1. Takhashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., Yamanaka S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131 (5): 861 - 872. 2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science, 318 (5858): 1917 – 1920. 3. Mallanna, S. and Rizzino, A. (2010) Emerging roles of microRNAs in the control of embryonic stem cells and the generation of induced pluripotent stem cells. Dev Biol. 344: 16-25. 4. Anokye-Danso, F., Trivedi, C. M., Juhr, D., Gupta, M., Cui, Z., Tian, Y., Zhang, Y., Yang, W., Gruber, P. J., Epstein, J. A., and Morrisey, E. E. (2011) Highly Efficient miRNA-Mediatd Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell Stem Cell. 8 (4): 376 – 388. 5. Miyoshi, N., Ishii, H., Nagano, H., Haraguchi, N., Dewi, D. L., Kano, Y., Nishikawa, S., Tanemura, M., Mimori, K., Tanaka, F., Saito, T., Nishimura, J., Takemasa, I., Mizushima, T., Ikeda, M., Yamamoto. H., Sekimoto, M., Doki, Y., and Mori, M. (2011) Reprogramming of Mouse and Human Cells to Pluripotency Using Mature MicroRNAs. Cell Stem Cell 8: 633 – 638. 6. Liao, B., Bao, X., Liu, L., Feng, S., Zovoilis, Z., Liu, W., Xue, Y., Cai, J., Guo, X., Qin, B., Zhang, R., Wu, J., Lai, L., Teng, M., Niu, L., Zhang, B., Esteban, M. A., Pei, D. (2011) MicroRNA Cluster 302–367 Enhances Somatic Cell Reprogramming by Accelerating a Mesenchymal-to-Epithelial Transition. The Journal of Biological Chemistry, 286, 17359 – 17364. 7. Kuo, C-H., Ying, S-Y. (2013) MicroRNA-Mediated Somatic Cell Reprogramming. Journal of Cellular Biochemistry, 114:275 – 281. PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Products for Reprogramming Reprogramming Systems (13) Transfection (2) Antibodies (19) Cell Lines (4) Media (3) Cytokines / Proteins (1) Small Molecules (5) Catalog # Brand Product Size Reprogramming Tools Reprogramming Systems 05-0018 05-0020 05-0015 05-0022 05-0017 00-0073 00-0067 Stemgent® Stemgent® Stemgent® Stemgent® Stemgent® Stemgent® Stemgent® c-Myc mRNA, Human eGFP mRNA Klf4 mRNA, Human L-Myc mRNA, Human Lin28 mRNA, Human microRNA Booster Kit mRNA Reprogramming Factors Set: hOKSML 20 μg 20 μg 20 μg 20 μg 20 μg kit 1 Set 00-0071 Stemgent® mRNA Reprogramming Kit 1 kit 00-0071mi Stemgent® microRNA-Enhanced mRNA Reprogramming Kit 1 kit 05-0021 05-0019 05-0014 05-0016 00-0068 Stemgent® Stemgent® Stemgent® Stemgent® Stemgent® Nanog mRNA, Human nGFP mRNA Oct4 mRNA, Human Sox2 mRNA, Human VSVg Retrovirus Reprogramming Set: Human OKSM 20 μg 20 μg 20 μg 20 μg 1 set Transfection 05-0020 00-0069 Stemgent® Stemfect™ eGFP mRNA RNA Transfection Kit 20 μg 1 Kit Antibodies 00-0009 Stemgent® Alkaline Phosphatase Staining Kit 50 assays PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Catalog # 00-0055 Brand Stemgent® Product Alkaline Phosphatase Staining Kit II Size 50 assays 09-0021 Stemgent® Klf4 Antibody (Purified), Mouse anti-Mouse/Human 100 μl 09-0020 Stemgent® Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human 100 μl 09-0023 Stemgent® Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human 100 μl 09-0024 Stemgent® Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human 100 μl 09-0067 StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse anti-Mouse/Human 100 μl 09-0095 StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human 100 μl 09-0001 Stemgent® SSEA-1 Antibody (PE), Mouse antiMouse/Human 1 ml 09-0005 Stemgent® SSEA-1 Antibody (Purified), Mouse anti-Mouse/Human 100 μl 09-0044 Stemgent® SSEA-3 Antibody (PE), Rat antiHuman 1 ml 09-0014 Stemgent® SSEA-3 Antibody (Purified), Rat anti-Mouse/Human 100 μl 09-0003 Stemgent® SSEA-4 Antibody (PE), Mouse antiHuman 1 ml 09-0006 Stemgent® SSEA-4 Antibody (Purified), Mouse anti-Human 100 μl PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Catalog # 09-0068 Brand StainAlive™ Product TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human Size 09-0009 Stemgent® TRA-1-60 Antibody (PE), Mouse anti-Human 1 ml 09-0010 Stemgent® TRA-1-60 Antibody (Purified), Mouse anti-Human 100 μl 09-0069 StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human 100 μl 09-0011 Stemgent® TRA-1-81 Antibody (Purified), Mouse anti-Human 100 μl 100 μl Cell Lines 08-0006 Stemgent® Mouse Primary iPS Cells-T1b 2 x 105 cells/vial 08-0007 Stemgent® Mouse Primary iPS Cells-WP5 2 x 105 cells/vial GSC-3002G Stemgent® Stemgent® NuFF-RQ™ IRR 4-5 x 106 cells/vial GSC-3006G Stemgent® Stemgent® NuFF-RQ™ IRR 4-5 x 106 cells/vial Media 01-0014-500 Stemgent® hES Cell Cloning & Recovery Supplement 5 x 100 μl 00-0070 Pluriton™ Reprogramming Medium 500 ml 01-0005 01-0005-100 NutriStem™ XF/FF Culture Medium 500 ml 100 ml Cytokines / Proteins 03-0002 Stemfactor™ FGF-basic, Human Recombinant 50 μg Small Molecules 04-0016 04-0017 04-0007 04-0012 04-0012-10 04-0012-02 Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Doxycycline hyclate Thiazovivin Valproic Acid Y27632 Stemolecule™ Y27632 in Solution 10 mg 1 mg 5g 2 mg 10 mg 2 mg (10 mM) PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Self-renewal Solutions from Stemgent The key feature of ES and iPS cells, that distinguishes them from all other cells, is the ability to proliferate indefinitely while maintaining the capacity to differentiate into many cell types. Therefore ES and iPS cells present an enormous potential as biological tools in strategies for regenerative medicine, drug discovery, disease models, as well as for research in developmental biology. However, physical and chemical stimuli from the environment influence these cells’ behavior by triggering signaling pathways that directs them into remaining a pluripotent state or differentiating into a mature cell type. This sensitivity of pluripotent stem cells towards environmental changes has proven to be a major challenge. A number of factors that should be considered for sustained culturing of ES and iPS stem cells include: 1. Defined cell culture medium for reproducibility 2. Defined media components at optimal concentrations; cytokines (growth factors) and small molecules 3. Optimal cell density 4. Methods for passaging cells that do not force differentiation 5. Defined basement membrane substrates that facilitate cell attachment ES and iPS cell cultures should be routinely monitored for pluripotency, because of their extreme sensitivity,. Some of the key assays include: 1. Normal colony morphology and rate of proliferation 2. Expression of key pluripotency genes and absence of lineage-specific genes (for iPS cells) 3. Expression of key pluripotency surface markers 4. Ability to differentiate into cells from all three primary germline layer tissues in vivo and in vitro 5. Normal genetic karyotype New technologies for stem cell research are being developed with increasing speed. Our teams at Stemgent work diligently with our Scientific Advisory Board and other leading stem cell scientists to validate and deliver cutting-edge reagents that enable you to more readily culture and characterize ES and iPS cells. Products for Self-Renewal Antibodies (19) Media (3) Cytokines / Proteins (4) PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Small Molecules (12) Catalog # Brand Product Size Self-Renewal Antibodies 00-0009 temgent® Alkaline Phosphatase Staining Kit 50 assays 00-0055 Stemgent® Alkaline Phosphatase Staining Kit II 50 assays 09-0021 Stemgent® Klf4 Antibody (Purified), Mouse anti-Mouse/Human 100 μl 09-0020 Stemgent® Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human 100 μl 09-0023 Stemgent® Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human 100 μl 09-0024 Stemgent® Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human 100 μl 09-0067 StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse anti-Mouse/Human 100 μl 09-0095 StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human 100 μl 09-0001 Stemgent® SSEA-1 Antibody (PE), Mouse antiMouse/Human 1 ml 09-0005 Stemgent® SSEA-1 Antibody (Purified), Mouse anti-Mouse/Human 100 μl 09-0044 Stemgent® SSEA-3 Antibody (PE), Rat antiHuman 1 ml PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Catalog # 09-0014 Brand Stemgent® Product SSEA-3 Antibody (Purified), Rat anti-Mouse/Human Size 09-0003 Stemgent® SSEA-4 Antibody (PE), Mouse antiHuman 1 ml 09-0006 Stemgent® SSEA-4 Antibody (Purified), Mouse anti-Human 100 μl 09-0068 StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human 100 μl 09-0009 Stemgent® TRA-1-60 Antibody (PE), Mouse anti-Human 1 ml 09-0010 Stemgent® TRA-1-60 Antibody (Purified), Mouse anti-Human 100 μl 09-0069 StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human 100 μl 09-0011 Stemgent® TRA-1-81 Antibody (Purified), Mouse anti-Human 100 μl 100 μl Media 01-0013-50 01-0014-500 CryoStem™ Stemgent® Freezing Medium hES Cell Cloning & Recovery Supplement 50 ml 5 x 100 μl 01-0005 01-0005-100 NutriStem™ XF/FF Culture Medium 500 ml 100 ml Cytokines / Proteins 03-0001 Stemfactor™ Activin A, Human Recombinant 5 μg 03-0007 Stemfactor™ BMP-4, Human Recombinant 10 μg 03-0002 Stemfactor™ FGF-basic, Human Recombinant 50 μg 03-0004 Stemfactor™ TGF-β1, Human Recombinant 5 μg Small Molecules 04-0003 04-0004 Stemolecule™ Stemolecule™ BIO CHIR99021 2 mg 2 mg PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Catalog # 04-0004-10 04-0004-02 04-0049 04-0006 04-0006-10 04-0006-02 04-0008 04-0030 04-0011 04-0017 04-0012 04-0012-10 04-0012-02 Brand Product Stemolecule™ Stemolecule™ Stemolecule™ CHIR99021 in Solution GSK429286A PD0325901 Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ PD0325901 in Solution PD173074 ROCK II Inhibitor SC1 (Pluripotin) Thiazovivin Y27632 Stemolecule™ Y27632 in Solution Size 10 mg 2 mg (10 mM) 2 mg 2 mg 10 mg 2 mg (10 mM) 2 mg 2 mg 1 mg 1 mg 2 mg 10 mg 2 mg (10 mM) Differentiaton Solutions from Stemgent The stem cell community has endeavored to find culture conditions and key signaling factors to guide stem cell differentiation towards clinically relevant cell fates, since the derivation of human embryonic stem cells in 1981. In vitro differentiation cell culture strategies were designed from signaling cues gained from in vivo development. Novel small molecules were discovered thanks to recent advances in high-throughput robotics and chemical synthesis. These molecules can be used alone or with more traditional cell culture reagents such as cytokines and extracellular matrix (ECM) substrates. We recommend to test the best ECM conditions first (MicroMatrix Arrays) as the microenvironment has a huge influence on stem cell differentiation. Stemgent offers consistent robust reagents for stem cell differentiation towards: 1. Ectoderm: with cytokines (e.g. SHH, Noggin), small molecules (e.g. SHH Signaling Pathway Modulator Set); and matrices (Laminin). 2. Endoderm: with cytokines (e.g. Activin A) and small molecules (e.g. IDE-1, IDE-2). 3. Mesoderm: with cytokines (e.g. BMP-4, Wnt-3a) and small molecules (e.g. Wnt Signaling Pathway Set) Inspired by the success of reprogramming mature cells to iPS cells, Marius Wernig2 and Douglas Melton3 have lead the way to identifying key transcription factors that directly differentiate skin cells to neurons (Ascl1, Brn2, and Myt1l) and pancreatic exocrine cells into insulin-producing beta-cells (Ngn3, Pdx1, and Mafa). Stemgent newly offers viral vectors for the directed differentiation of one mature cell type to another mature cell type, which reduces the experimental time between patient tissue collection and derivation of cell type of interest in regenerative medicine. PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Our teams at Stemgent work diligently with our Scientific Advisory Board and other leading stem cell scientists to validate and deliver cutting-edge reagents that enable you to more readily generate, differentiate, culture, and characterize ES/iPS cells for use in strategies in regenerative medicine, disease modeling, drug discovery, and developmental biology. References 1. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M. (1998) Embryonic Stem Cell Lines Derived from Human Blastocysts. Science, 282:1145– 1147. 2. Vierbuchen T., Ostermeier A., Pang Z.P., Kokubu Y., Südhof T.C., Wernig M. (2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 463(7284):103541. 3. Zhou Q., Brown J., Kanarek A., Rajagopal J., Melton D.A. (2008) In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Nature, 455(7213):627-32. Products for Differentiation Antibodies (1) Media (3) Cytokines / Proteins (6) Small Molecules (29) Differentiation (7) Catalog # Brand Product Size Differentiation Antibodies 09-0075 Stemgent® Pax6 Antibody (Purified), Rabbit anti-Mouse/Human 100 μl Media 01-0014-500 Stemgent® hES Cell Cloning & Recovery Supplement 5 x 100 μl 00-0072 Stemgent® MesoFate™ Differentiation Medium 500 ml + supplement 01-0005 01-0005-100 NutriStem™ XF/FF Culture Medium 500 ml 100 ml Cytokines / Proteins 03-0001 Stemfactor™ Activin A, Human Recombinant 5 μg 03-0007 Stemfactor™ BMP-4, Human Recombinant 10 μg PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Catalog # 03-0002 Brand Stemfactor™ Product FGF-basic, Human Recombinant Size 50 μg 03-0006 Stemfactor™ Noggin, Human Recombinant 10 μg 03-0008 Stemfactor™ Sonic Hedgehog, Human Recombinant 25 μg 03-0004 Stemfactor™ TGF-β1, Human Recombinant 5 μg Small Molecules 04-0052 04-0014 04-0014-10 04-0015 04-0021 04-0051 04-0003 04-0004 04-0004-10 04-0004-02 04-0022 04-0041 04-0024 04-0025 04-0026 04-0027 04-0037 04-0028 04-0050 04-0074 04-0074-10 04-0074-02 Stemolecule™ Stemolecule™ A769662 A83-01 Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ ALK5 Inhibitor All-Trans Retinoic Acid BI-D1870 BIO CHIR99021 Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ CHIR99021 in Solution Cyclopamine DAPT Dorsomorphin Forskolin IDE-1 IDE-2 IPA-3 KAAD-Cyclopamine Ku-0063794 LDN-193189 Stemolecule™ LDN-193189 in Solution 04-0008 04-0009 04-0010 04-0010-10 04-0010-05 04-0032 04-0031 04-0034 Stemolecule™ Stemolecule™ Stemolecule™ PD173074 Purmorphamine SB431542 Stemolecule™ Stemolecule™ Stemolecule™ Stemolecule™ SB431542 in Solution SHH Antagonist SMO Antagonist Wnt Inhibitor IWP-2 10 mg 2 mg 10 mg 1 mg 100 mg 10 mg 2 mg 2 mg 10 mg 2 mg (10 mM) 2 mg 5 mg 2 mg 10 mg 2 mg 2 mg 5 mg 100 μg 2 mg 2 mg 10 mg 2 mg (10 mM) 2 mg 5 mg 5 mg 10 mg 5 mg (10 mM) 2 mg 2 mg 2 mg PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Products 2015 Catalog # 04-0035 04-0036 04-0046 Brand Stemolecule™ Stemolecule™ Stemolecule™ Product Wnt Inhibitor IWP-3 Wnt Inhibitor IWP-4 XAV939 Size 2 mg 2 mg 2 mg mRNA´s for Differentiation 05-0033 05-0034 00-0072 Stemgent® Stemgent® Stemgent® Gata4 mRNA, Human Mef2c mRNA, Human MesoFate™ Differentiation Medium 20 µg 20 µg 500 ml + supplement 05-0031 05-0029 05-0032 05-0035 Stemgent® Stemgent® Stemgent® Stemgent® Myf5 mRNA, Human MyoD mRNA, Human Myog mRNA, Human Tbx5 mRNA, Human 20 µg 20 µg 20 µg 20 µg PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Prices 2015 sorted by Cat# Cat# Product Quantity 00-0009 00-0047-500 00-0051-500 00-0052-500 00-0055 00-0067 Stemgent® Alkaline Phosphatase Staining Kit WIT-T™ Culture Medium WIT-P-NC™ Culture Medium WIT-I-NC™ Culture Medium Stemgent® Alkaline Phosphatase Staining Kit II Stemgent® mRNA Reprogramming Factors Set: hOKSML Stemgent® VSVg Retrovirus Reprogramming Set: Human OKSM Stemfect™ RNA Transfection Kit Pluriton™ Reprogramming Medium Stemgent® mRNA Reprogramming Kit Stemgent® MesoFate™ Differentiation Media Stemgent®microRNA Booster Kit Renaissance™Essential Tumor Medium NutriStem® XF/FF Culture Medium NutriStem® XF/FF Culture Medium CryoStem™ Freezing Medium Stemgent® hES Cell Cloning & Recovery Supplement Stemfactor™ Activin A, Human Recombinant Stemfactor™ FGF-basic, Human Recombinant Stemfactor™ TGF-β1, Human Recombinant Stemfactor™ Noggin, Human Recombinant Stemfactor™ BMP-4, Human Recombinant Stemfactor™ Sonic Hedgehog, Human Recombinant Stemfactor™ LIF, Mouse Recombinant Stemfactor™ LIF, Mouse Recombinant Stemfactor™ LIF, Human Recombinant Stemfactor™ LIF, Human Recombinant Stemolecule™ RG108 Stemolecule™ BIX01294 Stemolecule™ BIO Stemolecule™ CHIR99021 Stemolecule™ CHIR99021 in Solution Stemolecule™ CHIR99021 Stemolecule™ Sodium Butyrate Stemolecule™ PD0325901 Stemolecule™ PD0325901 in Solution Stemolecule™ PD0325901 Stemolecule™ Valproic Acid Stemolecule™ PD173074 Stemolecule™ Purmorphamine Stemolecule™ SB431542 Stemolecule™ SB431542 in Solution Stemolecule™ SB431542 Stemolecule™ SC1 (Pluripotin) Stemolecule™ Y27632 50 assays 500 ml 500 ml 500 ml 50 assays 1 Set 00-0068 00-0069 00-0070 00-0071 00-0072 00-0073 00-0074 01-0005 01-0005-100 01-0013-50 01-0014-500 03-0001 03-0002 03-0004 03-0006 03-0007 03-0008 03-0011 03-0011-100 03-0016 03-0016-100 04-0001 04-0002 04-0003 04-0004 04-0004-02 04-0004-10 04-0005 04-0006 04-0006-02 04-0006-10 04-0007 04-0008 04-0009 04-0010 04-0010-05 04-0010-10 04-0011 04-0012 1 set 1 Kit 500 ml 1 kit 500 ml 1 kit 500 ml 500 ml 100 ml 50 ml 5 x 100 μl 5 μg 50 μg 5 μg 10 μg 10 μg 25 μg 1 ml, 10 μg/ml 1 ml, 100 μg/ml 1 ml, 10 μg/ml 1 ml, 100 μg/ml 10 mg 5 mg 2 mg 2 mg 2 mg (10 mM) 10 mg 500 mg 2 mg 2 mg (10 mM) 10 mg 5g 2 mg 5 mg 5 mg 5 mg (10 mM) 10 mg 1 mg 2 mg PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Price (EUR) 75,00 226,00 226,00 226,00 86,00 1348,00 644,00 247,00 202,00 1796,00 238,00 627,00 238,00 211,00 65,00 106,00 142,00 146,00 117,00 198,00 122,00 201,00 261,00 211,00 872,00 231,00 1176,00 126,00 98,00 98,00 187,00 219,00 805,00 51,00 187,00 219,00 805,00 51,00 117,00 98,00 94,00 94,00 155,00 98,00 117,00 Stemgent Prices 2015 Cat# Product Quantity 04-0012-02 04-0012-10 04-0013 04-0014 04-0014-10 04-0015 04-0016 04-0017 04-0021 04-0022 04-0024 04-0025 04-0026 04-0027 04-0028 04-0030 04-0031 04-0032 04-0033 04-0034 04-0035 04-0036 04-0037 04-0039 04-0041 04-0044 04-0046 04-0047 04-0049 04-0050 04-0051 04-0052 04-0054 04-0072 04-0074 04-0074-02 04-0074-10 04-0076 04-0077 04-0078 05-0014 05-0015 05-0016 05-0017 05-0018 05-0019 05-0020 Stemolecule™ Y27632 in Solution Stemolecule™ Y27632 Stemolecule™ R(+)Bay K 8644 Stemolecule™ A83-01 Stemolecule™ A83-01 Stemolecule™ ALK5 Inhibitor Stemolecule™ Doxycycline hyclate Stemolecule™ Thiazovivin Stemolecule™ All-Trans Retinoic Acid Stemolecule™ Cyclopamine Stemolecule™ Dorsomorphin Stemolecule™ Forskolin Stemolecule™ IDE-1 Stemolecule™ IDE-2 Stemolecule™ KAAD-Cyclopamine Stemolecule™ ROCK II Inhibitor Stemolecule™ SMO Antagonist Stemolecule™ SHH Antagonist Stemolecule™ Tranylcypromine hydrochloride Stemolecule™ Wnt Inhibitor IWP-2 Stemolecule™ Wnt Inhibitor IWP-3 Stemolecule™ Wnt Inhibitor IWP-4 Stemolecule™ IPA-3 Stemolecule™ Pifithrin-μ Stemolecule™ DAPT Stemolecule™ KP372-1 Stemolecule™ XAV939 Stemolecule™ GDC-0941 Stemolecule™ GSK429286A Stemolecule™ Ku-0063794 Stemolecule™ BI-D1870 Stemolecule™ A769662 Stemolecule™ LY411575 Stemolecule™ Pyrintegrin Stemolecule™ LDN-193189 Stemolecule™ LDN-193189 in Solution Stemolecule™ LDN-193189 Stemolecule™ PS48 Stemolecule™ AM580 Stemolecule™ CD437 Stemgent® Oct4 mRNA, Human Stemgent® Klf4 mRNA, Human Stemgent® Sox2 mRNA, Human Stemgent® Lin28 mRNA, Human Stemgent® c-Myc mRNA, Human Stemgent® nGFP mRNA Stemgent® eGFP mRNA 2 mg (10 mM) 10 mg 5 mg 2 mg 10 mg 1 mg 10 mg 1 mg 100 mg 2 mg 2 mg 10 mg 2 mg 2 mg 100 μg 2 mg 2 mg 2 mg 10 mg 2 mg 2 mg 2 mg 5 mg 5 mg 5 mg 5 mg 2 mg 5 mg 2 mg 2 mg 10 mg 10 mg 5 mg 1 mg 2 mg 2 mg (10 mM) 10 mg 5 mg 5 mg 5 mg 20 μg 20 μg 20 μg 20 μg 20 μg 20 μg 20 μg PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Price (EUR) 129,00 517,00 145,00 112,00 413,00 98,00 51,00 237,00 88,00 155,00 107,00 145,00 230,00 230,00 239,00 230,00 230,00 230,00 51,00 98,00 98,00 98,00 98,00 79,00 136,00 309,00 230,00 173,00 239,00 173,00 497,00 211,00 230,00 249,00 187,00 219,00 805,00 79,00 111,00 129,00 229,00 229,00 229,00 229,00 229,00 229,00 229,00 Stemgent Prices 2015 Cat# Product Quantity 05-0021 05-0022 05-0029 05-0031 05-0032 05-0033 05-0034 05-0035 08-0006 Stemgent® Nanog mRNA, Human Stemgent® L-Myc mRNA, Human Stemgent® MyoD mRNA, Human Stemgent® Myf5 mRNA, Human Stemgent® Myog mRNA, Human Stemgent® Gata4 mRNA, Human Stemgent® Mef2c mRNA, Human Stemgent® Tbx5 mRNA, Human Stemgent® Mouse Primary iPS Cells-T1b 08-0007 Stemgent® Mouse Primary iPS Cells-WP5 09-0001 Stemgent® SSEA-1 Antibody (PE), Mouse antiMouse/Human Stemgent® SSEA-4 Antibody (PE), Mouse antiHuman Stemgent® SSEA-1 Antibody (Purified), Mouse antiMouse/Human Stemgent® SSEA-4 Antibody (Purified), Mouse antiHuman Stemgent® TRA-1-60 Antibody (PE), Mouse antiHuman Stemgent® TRA-1-60 Antibody (Purified), Mouse anti-Human Stemgent® TRA-1-81 Antibody (Purified), Mouse anti-Human Stemgent® SSEA-3 Antibody (Purified), Rat antiMouse/Human Stemgent® Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human Stemgent® Klf4 Antibody (Purified), Mouse antiMouse/Human Stemgent® Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human Stemgent® Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human Stemgent® Goat anti-Rabbit IgG Antibody (DyLight™ 488) Stemgent® SSEA-3 Antibody (PE), Rat anti-Human StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse anti-Mouse/Human StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human StainAlive™ Mouse IgM, κ Isotype Control (DyLight™ 488) 20 μg 20 μg 20 µg 20 µg 20 µg 20 µg 20 µg 20 µg 2 x 105 cells/vial 2 x 105 cells/vial 1 ml 09-0003 09-0005 09-0006 09-0009 09-0010 09-0011 09-0014 09-0020 09-0021 09-0023 09-0024 09-0034 09-0044 09-0067 09-0068 09-0069 09-0072 Price (EUR) 229,00 229,00 229,00 229,00 229,00 229,00 229,00 229,00 474,00 474,00 151,00 1 ml 151,00 100 μl 170,00 100 μl 161,00 1 ml 133,00 100 μl 186,00 100 μl 96,00 100 μl 133,00 100 μl 244,00 100 μl 133,00 100 μl 170,00 100 μl 157,00 200 μl 80,00 1 ml 100 μl 142,00 238,00 100 μl 238,00 100 μl 238,00 100 μl 97,00 PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland Stemgent Prices 2015 Cat# Product Quantity 09-0075 Stemgent® Pax6 Antibody (Purified), Rabbit antiMouse/Human StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human StainAlive™ SSEA-4 Antibody (DyLight™ 550), Mouse anti-Human 100 μl Price (EUR) 244,00 100 μl 238,00 100 μl 238,00 09-0095 09-0097 Contact in Austria our partner HVD Life Science Vertriebs GmbH Wurzbachgasse 18 1152 Wien (P) +43 1 98 295 26 30 | (F) +43 1 786 36 44 Email: office1@havdgmbh.com Terms and Conditions / Lieferbedingungen Prices are valid from February 1st, 2015. All prices are net prices excluding tax and any applicable duties. All prices are subject to change without prior notice. Terms of delivery: EXW, Martinsried Germany. Errors and misprints are excepted. Die Preise sind gültig ab dem 1. Februar 2015. Preise: zzgl. MwSt.; Lieferung: ab Werk Martinsried, Deutschland , zuzüglich Transport- und Kühlgebühren Alle Preise können ohne Ankündigung geändert werden. Irrtümer und Druckfehler vorbehalten. All products are for research use only. Not for use in humans! PELOBIOTECH GmbH Am Klopferspitz 19 | 82152 Planegg | Germany (P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: info@pelobiotech.com | www.pelobiotech.com Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
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