Sengupta, M. and Dalwani, R. (Editors). 2008. Proceedings of Taal2007: The 12th World Lake Conference: 167-175 Water Quality Monitoring and Assessment: Current Status and Future Needs Richard D. Robarts1, Sabrina J. Barker2 and Scott Evans 1 UNEP GEMS/Water Programme, 11 Innovation Blvd., Saskatoon, SK, CANADA S7N 3H5 2 UNEP GEMS/Water Programme, c/o UNEP-DEWA, 1 United Nations Av., Gigiri, Nairobi, Kenya 3 Chipotle Business Group, Inc. 121 West Park Row Drive, Arlington, Texas, 76010 U.S.A. Email: richard.robarts@gemswater.org, sabrina.barker@gemswater.org, sevans@chipotlegroup.com ABSTRACT Although water quality monitoring and assessment programmes provide essential background information to scientific research, policy makers and water mangers and keep societies informed of the state and trend of the Earth’s vital aquatic ecosystems, their value has at times been called into question for very good reasons. This is because monitoring programmes can become self-generating unless they are strongly linked to the needs of users and can generate large amounts of data at great cost that are unnecessary or are not used. In many countries decisions were made to cancel monitoring programmes where 20 years or so ago, these have been re-instated as environmental issues become an increasing concern worldwide. Modern monitoring programmes are designed to be effective keystone components of integrated water resources management. With the use of interoperable database technology, data can be shared amongst a wide number of users, providing a strong return on investment for governments and society generally. However, costs remain high for a modern water quality programme and there are remote areas where it is still not possible to monitor water quality; in some countries even simple monitoring programmes are beyond local means. Continued research and development of innovative new technology, therefore, are necessary to adequately meet the demands for environmental water quality information. We believe the future for innovative water quality testing technologies looks bright. Keywords: inland waters, lakes, climate change, data sharing, data bases, open web services, monitoring technologies INTRODUCTION Key environmental stressors Fresh water, like all natural resources, is under increased demand as the world’s population grows. In many regions of the world people are removing water from rivers, lakes and aquifers faster than these systems can be recharged. It has been estimated that population growth alone will mean that the number of water-stressed, or water-scarce, countries will increase from 31 to 48 within the next 30 years (Hinrichsen et al. 1998). Additionally, the demand for fresh water has increased in response to industrial development, increasing reliance on irrigated agriculture, massive urbanization, and rising living standards (Shiklomanov 2000). Global freshwater availability is shrinking not only in quantitative terms, but also in qualitative terms because many freshwater systems have become increasingly polluted with a wide variety of human, agricultural and industrial wastes (Shiklomanov 2000). In addition, climate change and variability are also expected to affect both the quantity and quality of water, creating competing demands for this resource from multiple sectors of society. Developing countries are faced with difficult choices as they find themselves caught between finite and increasingly polluted water supplies on the one hand, and rapidly rising demand from population growth and development on the other (Somlyódy et al. 2001). Water shortages and pollution are causing widespread public health problems, limiting economic and agricultural development, and harming a wide range of ecosystems, which may result in a series of local and regional water crises with global implications (CSD 1997). However, water quality has improved in many systems when local political will has resulted in resources and management plans bring necessary positive changes (UNEP GEMS/Water Programme 2007). Lakes, unlike rivers, are mainly storage bodies and are estimated to contain more than 90 per cent of the liquid freshwater on Earth (ILEC 2003). Lakes are dynamic ecosystems, and in addition to their storage function they are the source of food and recreation for humans, support a large range of biodiversity goods and services and provide the foundation for people’s livelihoods. When natural climatic conditions do not provide enough water, lakes can meet both human and ecosystem needs (ILEC 2003). Unfortunately, lakes are also among the most vulnerable and fragile aquatic ecosystems as they are a sink for a wide range of dissolved and particulate substances. In addition, climate change can interact with other lake stressors with both positive and negative consequences for specific lake systems, as outlined in Table 1. Table 1. The interaction of climate change with other lake stressors (from Schindler, 1997). • • • • • • • • Climatic warming may delay the recovery of acidified lakes. Decreasing DOC concentrations in warming lakes could be accelerated by acidification. Eutrophication problems may increase, even though nutrient loads may decrease because of increased retention times. Dissolved oxygen saturation decreases with increasing temperature so that the impact of oxygen consuming effluents may be more severe. Increased periods of stratification in eutrophic lakes could exacerbate hypolimnetic oxygen deficits. Increased UV radiation exposure of organisms may occur in lakes undergoing warming and acidification due to decreasing DOC concentrations. Climate warming interactions with toxins will be many and complex, ranging from reduced toxin loads, increased retention, to increased revolatilization, decomposition and cycling in lakes. Increased human use of water will interact with climate warming to compound already severe problems in water quantity and quality, which will impact all aspects of society and the environment. Reliable, consistent and appropriate information is the key to understanding and improving the world’s supply and quality of freshwater. There is a general consensus that our knowledge of the state of the world’s freshwaters needs to improve (WWAP 2006). Inland and marine waters are intimately linked in the hydrological cycle so that an improvement in our knowledge of the quality of inland waters will also lead to benefits for the marine environment. The need for water quality monitoring and assessment programmes and the key attributes of successful programmes Lovett et al. (2007) define environmental monitoring as a time series of measurements of physical, chemical and/or biological variables designed to answer questions about environmental change. Water quality monitoring programmes are essentially of two types: those in support of research, and those that provide essential information and assessments for management and policy needs. However, almost a decade ago long-term monitoring programmes fell 168 out of favour with some government organizations because: • Monitoring was viewed by some as not being real science, but only a fishing expedition that diverts resources from “real” science (Lovett et al. 2007). • Programmes were large and expensive requiring sophisticated and expensive equipment. • They were disconnected from the purpose of science or management as a result of organizational fragmentation. • They became self-focused and their own sole purpose, i.e., monitoring for the sake of monitoring. • Too many and unnecessary parameters were measured too frequently at too many stations. As new analytical technologies have become available it has become easier to measure large numbers of variables in water samples, which can lead to the collection of data that are unnecessary although at no real extra cost. • Large amounts of data were collected but not used. • We cannot know today what crucial questions will need to be answered in the future. As a result, only governments or large corporations could afford to do monitoring. Even so, monitoring programmes in some countries were abruptly terminated without due consideration for the consequences (Fig. 1). But water quality monitoring when properly designed and integrated into decisionmaking processes as outlined below provides crucial information for the development of policies and management plans that protect and preserve our essential aquatic ecosystems. Indeed as Lovett et al. (2007) have noted, there are many highly successful long-term monitoring programmes that have provided important scientific advances and crucial information for environmental policy. However, they need to: • Be tailor-made for a country’s needs based on knowledge of the variance in local aquatic systems and the known and potential stressors. • They do not have to be large and expensive – small but effective monitoring programmes can be undertaken by groups at all levels, including trained high school children using simple, low cost test kits. • Knowledge is power, so the right information presented appropriately is essential to protecting and conserving inland waters and getting political action. • Information must be freely shared with all stakeholders. 30500 0.20 Nitrate + Nitrite Instantaneous Flow 0.16 24400 0.12 18300 0.08 12200 0.04 6100 0.00 1979 1980 1981 1982 1983 1984 1988 1989 1990 1991 1992 Instantaneous Flow (m 3 sec -1) Nitrate + Nitrite (mg L-1) Legend 0.00 Year Figure 1 An example from the Mackenzie River in Canada of a long-term break in water monitoring as a result of a decision to place scarce resources into other activities and its re-instatement when it was realized that this prevented the detection of possible impacts with growing downstream economic activities. A number of attributes of successful monitoring programmes that we have previously identified are: 1. Identify management and policy information needs. 2. Link to institutional arrangements with regulatory ability (i.e., to establish standards). 3. Improve coordination among the organizations involved in water, sanitation and ecosystem and human health. 4. Define data and information needs and then design the monitoring network to meet them. 5. Ensure reliable and timely data collection and reporting. 6. Co-locate water quality and quantity stations for the calculation of fluxes. 7. Enough monitoring stations strategically located to have accurate and reliable country/basin coverage, i.e., a headwater stations and another station where a river enters the marine environment, a large inland water body or crosses an international border. 8. Be responsive to unexpected problems and emerging issues. 9. Maintain country needs by building capacity and empowerment. 10. Strengthen existing network infrastructure and institutions rather than creating new ones. 11. Promote free access to information through the interoperability and comparability of methods. 12. Maintain systems up-to-date (IT, analytical etc.). Moving from monitoring to prediction In a recent article describing the U.S. Geological Survey’s perspective on water quality monitoring and assessment in the United States, Hirsch et al. (2006) noted that the development and verification of predictive tools and models is essential to understand and successfully manage the country’s waters. It is not financially feasible to establish a detailed network of monitoring stations in very large countries, a problem faced by the UNEP GEMS/Water Programme in trying to compile representative water quality data for the world. Therefore, as Hirsch et al. point out, it is necessary to get smarter by enhancing the value of data collected at individual sites and applying, both spatially and temporally, our understanding of hydrological systems and water quality conditions to larger areas. Development of appropriate models is the key to doing this. At the same time, development of predictive tools will help to prioritize contaminant sources and to identify the relative importance of the many factors that influence water quality at different geographical scales. Models can also be used to estimate probabilities that a concentration of a specific compound will exceed guidelines and standards for the use of drinking, agricultural and recreational waters use (Hirsch et al. 2006). It is essential however, that reliable, comparable and comprehensive data must continue to be generated by traditional monitoring methods. These data will be 169 required to validate and verify model outputs and concomitantly reduce their uncertainty. The benefits of water quality monitoring and assessment programmes Why long term monitoring programmes? Many aquatic systems may change only slowly and the long-term record of key parameters provides the essential yardstick to assess status and trends. This is especially important now as we try to assess and predict the impact of climate change and variability on inland water systems. Long term programmes may also identify previously unknown “hotspots” and point to developing issues. Data provided by such programmes are also necessary to determine whether an event or change is normal, unusual or extreme (Lovett et al. 2007) and can contribute to the development of a scientific project or a more detailed monitoring activity. Unfortunately, long-term monitoring records are rare due to the lack of resources available to sustain them, but they are extremely valuable when they are made available. The United Nations Environment Programme (UNEP) GEMS/Water Programme (www.gemswater.org) collects water quality data at a global scale and maintains an on-line database (GEMStat) with almost four million entries covering the period 1965 to 2007 (www.gemstat.org). And, while the database contains water quality monitoring records from over 80 participating countries, regular long term reporting of monitoring data to GEMS/Water is the exception rather than the rule: many countries report regularly for only a few years, and sometimes country records span two distinct periods that often are separated by more than a decade where water quality data were not reported to GEMS/Water (Fig. 2). As noted by Lovett et al. (2007), monitoring programmes not only provide the basis of the formulation of science-based environmental policies, but also continued monitoring makes possible evaluations that determine whether or not a policy has resulted in the desired effect and been cost effective. It is well known that often the prevention of an environmental problem is less costly than its remediation. While solutions for many environmental problems are expensive and technically challenging, what is often not recognized is that the cost of well-designed monitoring programmes is generally much less than either the cost of policy implementation or the monetary benefits associated with the environmental improvement (Lovett et al. 2007). Figure 2. Some examples of variable data contributions from countries participating in the UNEP GEMS/Water Programme. These breaks in data are due to a number of factors such as national decisions to change monitoring stations in response to local priorities or changes in agencies responsible for monitoring with a resulting loss of contact between the Programme and a national focal point. 170 Data and information sharing and use It is known that water quality issues are site-specific, and that actions at local levels are needed to deal with any particular watershed. Yet at the same time, local actions and regional activities can greatly benefit from linkages at the global level, such as: sharing experiences between sites and regions; exchange of data and information on global scientific and Internet developments; and situating water quality data within a broader geospatial context for analysis and assessment. Many policy- and decision-makers at local, national and regional levels are concerned about investing adequately in data collection systems. Many governments around the world are becoming increasingly interested in developing national data and information systems, as well as in ensuring their interoperability. Interoperability refers to the ability of a database or system to exchange information and to use the information that has been exchanged. Often this is achieved using Open Web Services. One example is the online water quality database, GEMStat introduced above. GEMStat provides environmental water quality data and information of the highest integrity, accessibility and interoperability. These data serve to strengthen the scientific basis for global and regional water assessments, indicators and early warning. The GEMStat website is designed to share surface and ground water quality data sets collected from the GEMS/Water Global Network, including over 2,900 stations, almost four million records, and over 100 parameters. The success of the database depends on the important role that partners in developing and transitional countries play in regional and global water information systems, to ensure the widest coverage possible, and consequently, the ability to share data and information. It also depends on the widest accessibility to stakeholders and beneficiaries. For these reasons, in March 2006, GEMStat was expanded as an Open Web Service. Open Web Services enable GEMStat to respond not only to users’ requests for water quality data, but also to requests coming from other computer systems. These other computer systems could include UNEP's environmental assessment database, the Global Environment Outlook data portal; ECOLEX, the international database of environmental law jointly run by IUCN, FAO and UNEP; or the endangered species and protected areas databases operated at UNEP-WCMC on behalf of IUCN, UNESCO and other partners. Open Web Services also allow other agencies and researchers to incorporate GEMStat data in their own research and assessments, with less demand on GEMS/Water to select and prepare the data. The result will be more extensive and more frequent use of GEMStat data, and more feedback from users to ensure the quality and utility of these data. Open Web Services refer to using the World Wide Web so that database services, like GEMStat, can promote their presence and capabilities, and other services can find and connect to them. Standards and specifications are developed by industry, academic, governmental and other interested parties. These groups include the Open Geospatial Consortium, OASIS, and the Open Archive initiative, and the specifications they develop are published openly, for use by their members or anyone else at no cost. The outcome is flexibility to identify and fit services to particular needs. Many instances of this on-the-fly integration of services have taken place, such as the response to the Indian Ocean tsunami and Hurricane Katrina, where pre-existing state, national and international information systems were able to be quickly co-opted into generating overviews combining everything from satellite images of weather systems down to locations of individual rescue teams, all through the Web. This is only one example to illustrate how accessing GEMStat stations by Open Web Services and Google Earth can be used to assist researchers and analysts to consider every station in its geographical context (Fig. 3). It can also serve to compare stations in different parts of the world that share for example, similar ecosystems. This application is particularly interesting for assessments on water quality and erosion, as “upstream” or nearby land characteristics can be easily determined. Figure 3 Distribution of UNEP GEMS/Water Programme monitoring stations in India. Monitoring stations for all countries participating in GEMS/Water can visualized with Google Earth through www.gemstat.org. There are 263 international river basins in the world, which account for significant land coverage and importance for many people. Thus, it is the political composition of these shared waters that highlights the many complexities involved with transboundary water management. Many governments have recognized the need for international cooperation through treaties and 171 agreements to effectively govern their transboundary watersheds. Including integrated water resource management (IWRM) approaches at the basin level, competing water demands can be better understood and managed. International cooperation with water resources also creates improved conditions for trade and investment. Further research is needed to more rigorously describe the relationship between socioeconomic development and water resource health. New technologies for water quality monitoring In addition to the importance of being able to access existing water quality data sources, new technologies for water quality monitoring that are rapid, quantitative, field deployable, comprehensive, simple to use, and cost effective are needed to increase the availability of world-wide water quality data. Currently, data gaps exist in many regions without national water quality monitoring programmes and existing surveys do not necessarily provide essential quantitative information on source or end-user water quality (WHO/UNICEF 2004). Furthermore, the need for new water quality assessment tools is not limited to developing countries. In the United States there is an outcry for new beach water quality monitoring tools and standards which will ensure the safety of humans and preserve natural resources (Dorfman 2006). Current technologies to analyze water for bacterial contaminates require an incubation time between 24 and 48 hours (Messer and Dufour 1998; USEPA 2000), even though studies have shown that temporal changes in indicator bacteria levels in beach water occur on much shorter time scales (Leecaster and Weisberg 2001; Boehm et al. 2002). Often, by the time test results are returned to decision makers, the threat of exposure to contaminated water has passed. Even the most basic water quality test kits for physical-chemical properties utilizing reagents and colorimetric devices can be cumbersome, time consuming, expensive, require skilled operators, and have a high degree of human error. For instance, the HACH DR 5000 spectrophotometer, commonly used in the US by public laboratories for water quality analysis, can analyze for up to a maximum of 90 single parameters in an 8 hour work day. However, to acquire some measure of quality data a laboratory provides, water samples must be transported to the laboratory for analysis, adding additional time, and in some cases sample degradation, between actual collection of the water sample and the final results. Currently, many countries do not have adequate water quality monitoring programmes due to not only the high initial capital expenditure in setting up an environmental laboratory, but also to costs including equipment maintenance, consumables, staffing, and quality control. For instance, the Texas Commission on Environmental Quality, the State’s 172 regulatory agency, has a US $480.7 million operating budget for the 2007 fiscal year (http://www.tceq.state.tx.us/), nearly half the entire Gross Domestic Product of the Republic of GuineaBissau, a western African nation (http://en.wikipedia.org/wiki/Comparison_between_ U.S._states_and_countries_by_GDP_%28PPP%29). Clearly, developing nations cannot invest in such extensive water quality monitoring, yet there are few options for reliable monitoring results with low capital investment. Development of novel, accurate, and precise tests for the detection of physical-chemical properties, biologicals or pollutants in water has mushroomed in the past decade as new technologies have become available. One of the most promising advances, the Sensicore WaterPOINT 870 Multi-Parameter Optical Water Quality Analyzer based on lab-on-a-chip technology, was introduced in 2006 and boasts up to 24 different physical-chemical results in just a few minutes. Other recent technologies used for physical-chemical detection include flow-injection immunoassays (Hennion and Barcelo 1998), dipstick immunoassays (Hennion and Barcelo 1998), test strips coated with colloidal gold particles (Verheijen et al. 2000; Putalun et al. 2004), liposome-amplified immunoassays, electrochemical immunoassays (Kašná and Skládal 2002) chemi-luminescent immunoassays (Oi and Zhang 2004), magnetic immunoassays (Liberti et al. 1997), and surface plasma resonance immunoassays (Svitel et al. 2000; Shimomura et al. 2001). Developing technologies for measuring microbial contaminants of waters include: 1) new enzyme/substrate methods that incorporate high-sensitivity fluorescence detection instruments, including dual wavelength fluorometry to simultaneously assess both enzymatic hydrolysis and the loss of substrate (Jadamec et al. 1999), 2) quantitative Polymerase Chain Reaction (qPCR) technology that relies on specific nucleic acid sequences (Noble et al. 2006), and antibody-antigen binding properties, which includes evanescent wave fiber optic biosensors (Wadkins et al. 1995), and 3) Rapid Bacteria Detection (RBD) system which is based on laser flow-through technology (Nobel and Weisburg 2005), and capture of the antigen by antibodies on magnetic beads (Lee and Deininger 2004). Unfortunately, all of the methods currently being developed for chemical and microbial analysis rely on sophisticated, expensive, lab-based equipment and highly skilled operators. New technologies must address limitations with current technologies to be effectively adopted into a water quality monitoring programme. For instance, for a new technology to add value to a monitoring program it must either be faster, more portable, more user friendly, more accurate, more cost effective, and/or produce a broader range of parameters sought by water quality monitors. While the aforementioned technologies are steps in the right direction, they fail to address a complete solution, that is, 1) rapid detection of both organic and inorganic elements in water; 2) field deployable; 3) operator friendly; 4) comprehensive; and perhaps most important, 5) cost effective. There is currently no single supplier or known technology capable of performing analyses for both physical-chemical and microbial properties in environmental water samples. Chipotle Business Group, Inc. (CBGI) intends to provide in thirdquarter 2008 the first water testing system capable of performing multiple immunological and reagent assays, side by side up to 100 total assays, simultaneously using the same quantitative optical detector, thus allowing a much easier, faster, cost effective, and comprehensive testing method. CBGI combines the miniaturization of current reagent assays with a proprietary immunological assay; both types of analyses are preformed simultaneously in a purpose-built detector utilizing quantitative optical analysis as depicted in Fig. 4. CBGI’s miniaturization of standard reagent tests for physicalchemical contaminants and packaging those tests into a multiple parameter cassette (Fig. 5) that can be read colourimetrically is ingenious. In doing so, CBGI not only increases the utility of colourimetric testing but also removes much of the human factor and costs associated with water analysis and current technologies. CBGI technologies can be packaged as portable field-deployable units about the size of a medium briefcase, submersible autonomous monitoring units, in-line process flow units, and free-standing laboratory units. Portable/field deployable units and continuous submersible autonomous monitoring stations will allow for: 1) the collection of data in countries with no programme or laboratories; 2) extend existing monitoring areas without greatly increasing costs; and 3) ensure uniformity of quality data, especially for large countries with many regional labs of different standards. CBGI predicts these technologies, once completely developed, could be a complete water quality monitoring solution for developing countries. Figure 5. Disassembled multiple parameter cassettes. Each of the 100 wells represents an independent assay that can either be a reagent assay or high concentration immunoassay. A second cassette is required for very low concentration immunoassays and utilizes a standard 100 mL sample size. Figure 4. Conceptual models of current water quality analyses and the CBGI approach to water quality analysis. There are literally thousands of emerging contaminates not currently regulated due to the lack of sound data for them and their effects on the environment and human health. The rapid detection immunoassays CBGI is developing for organic contaminants, such as estrogen and pesticides, will allow field staff to rapidly obtain essential data; thereby providing an early warning system and the development of an understanding that such contaminates may pose. This will lead to effective policies and management plans. Beyond being a comprehensive solution to water analysis, CBGI is developing a scientific breakthrough with its 30 minute immunoassay of very low concentration biologicals such as E. coli and Enterococcus. Rapid, accurate measurement of these contaminates is a great concern for not only developed countries but also for developing ones where water contamination by faecal bacteria poses a much greater danger to human health. CBGI is developing quantum dot technology to eliminate long incubation requirements for these assays, thus 173 allowing for a 30 minute time-to-results. This rapid detection, combined with the ability to test for these organisms in the field by unskilled or semi-skilled operators, will be a groundbreaking achievement. CONCLUSIONS Who needs water quality monitoring and assessment programmes? The answer is we all do: scientists to provide essential background information to their research, policy makers and mangers to design, implement and assess the efficacy of their work and society so that it remains informed of the state and trend of its vital aquatic ecosystems. Although monitoring programmes provide essential information to assess status and trends and to identify emerging issues and environmental hotspots in inland systems they can be highly expensive and ineffective if the information and data collected are not widely shared and used. Many policy- and decision-makers at all levels are concerned about investing adequately in data collection systems. Governments around the world are becoming increasingly interested in developing national data and information systems, as well as in ensuring their interoperability, or the ability of a database or system to exchange information and to use the information that has been exchanged. This is now commonly done through Open Web Services. Many national monitoring organizations and the UNEP GEMS/Water global monitoring programme are limited by current in-situ technologies, the parameters they are capable of detecting and their high cost. Laboratory analyses, while comprehensive, are very expensive, labour-intensive and require skilled technicians. For many countries, this is not an option in the foreseeable future. Continued research and development of innovative new technology, such as that proposed by CBGI, are necessary to adequately meet the demands for environmental water quality data. The future for innovative water quality testing technologies looks bright. However, one must take into consideration what motivates private industry to develop and introduce new technologies. Unlike the telecommunications and computer software industries where innovation is a requirement for survival, commercialization of groundbreaking innovation in the water quality testing industry is typically carried out by start-up firms like Sensicore and Chipotle Business Group, Inc. mentioned above. There are many new technological developments locked up in universities throughout the world, like most mentioned above, and it is well known that getting these technologies from a university into the hands of those who need them is not an easy endeavor. Case in point, lab-on-a-chip technology has been in existence for decades yet until only recently has found its way to water quality testing. Until those with money (governmental and 174 non-governmental organizations, philanthropic organizations, and private investors) take into account non-monetary benefits and transfer that money to those who can commercialize these technologies, technology development for water quality analysis will be at a lessened pace. ACKNOWLEDGMENTS We thank Nancy Brown-Peterson, Genevieve Carr, Kelly Hodgson and Joseph Zirnhelt for providing figures and comments on an earlier draft of the manuscript. REFERENCES Boehm, A.B., S.B. Grant, J.H. Kim, S.L. Mowbray, D.C. McGee, C.D. Clark, D.M. Foley and D.E. 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