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Sequence-based genotyping (SBG)
Genotyping made easy
How can I identify molecular markers for
my breeding program without any prior
genome or sequence information ? Are
there marker types which can directly be
linked to the genome sequence of my
crop ? These and others questions from
Sharifah Shahrul Rabiah Syed Alwee, breeders and crop researchers can be
answered by the genotyping technology
R&D, Felda Global Ventures
developed by KeyGene: Sequence-based
Genotyping (SBG). The applications are
numerous.
“We used SBG for the
identification of molecular
markers to speed up our oil
palm breeding program”
The sequence-based genotyping
technology is powerful by its simplicity.
One experiment in which simultaneously
SNP discovery and genotyping is
performed. Within four steps from DNA to
SNPs: (1) genome complexity reduction by
digestion of the DNA, (2) ligation of
adapters containing a sample-ID followed
by amplification, (3) pooling of the
samples and sequencing and (4) mining of
the data for polymorphisms.
Some applications of this genotyping
technology are: co- dominant SNP
genotyping, genetic diversity analysis,
genetic mapping, discovery of (rare) SNP
variants and genome wide association
mapping. The technology is offered in
contract research projects, as license
opportunity and as a kit for DNA template
preparation.
Can we support you with your
genotyping?
For more information, please contact:
Herco van Liere
VP Business Development
herco.van-liere@keygene.com
T +31 (0)317 466 866
Or visit: www.keygene.com
Sustainable crop innovations
Trade and/or brand names may be (registered) trademarks of their
respective owner. Sequence-based Genotyping is covered by patents
and patent applications owned by Keygene N.V.