1. No category

Portable device
for personal ecology and medicine
Ivan A Denisov
Lead engineer of MOLPIT
+7 (960) 769-07-25
denisov@molpit.com
The problem
A growing need in bioassay technologies for:
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●
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rapid detection of pollutants in water;
detection of poor quality products;
providing schools and universities with modern lab activities.
Limitations of the current bioassay methods:
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chemical analysis doesn’t show toxicity effects on biological system.
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living organisms tests are expensive, give high measurement error and
hard to use;
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the cost of sensitive enough luminometers is too high;
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the lack of affordable for consumer diagnostic devices.
2
Problem solving
Compact
diagnostic
device
Special Component
Software
Enzyme
biomodule
Modern solid-state
detectors
Microelectronics for
digital processing of
signals
3
Solution: enzyme biotests
Bioluminescent Enzyme System
Technology (BEST) allows to
carry out express bioassays of water
and physiological fluid for pollutants
Advantages of bioluminescent test:
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Broad screening – 25,000 toxins
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Rapid/emergency screening adaptability
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Focused toxin-class identification systems
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The method is compatible with standard laboratory
equipment
Shelf-life of reagents up to one year
4
Solution: LumiShot luminometer
Portable luminometer for high school and
university practical course. This cuvettebased device is suitable for routine
bioassay in bioluminescent research.
metal cover
сuvette
sample compartment
detection module
сuvettes
body cover
hinge system
electronics units
device body
miniUSB-port
hole for fixation
cuvettes and device support
5
Market segments
education
and research
environmental
monitoring
bioassay of
food products
6
The market
Luciferase enzymes and other enzymes $100M.
Chemical and biological agent detection $640M
Schools
Middle class families
USA
Europe
Revenue Forecasts
Asia
Russia
~53 000
~5,4 M
Analytical devices market
* Frost & Sullivan, 2010
7
Sensitivity, moles of luciferase
Comparison with competitors
1E-21
Promega
GloMax 20/20
1E-18
1E-15
Nauka BLM
3606m1
Berthold
Tristar
Nauka BLM
8802mpt
LumiShot
Berthold Lumat
LB 9507
Berthold
Junior LB 9509
1E-12
Higiena
SystemSure III
MSU Lum1
1E-9
Biotox-10M
$850
$2,000
$3,500
$5,000
Cost
$10,000
$100,000
8
The product distribution plan
Internet-shop
LumiShot
Distributor of
school equipment
Reagents
Schools and
universities
9
R&D pipeline
April-May
Trial sales and presales
June-July
Bug fixes & development of production roadmap
August-September
Start of mass production
October-December
Sales & support
LumiShot
10
The team
Elena N. Esimbekova
h.i.=8, publications 50+
Senior researcher
Prof. Valentina A. Kratasyuk,
Dr.Sc., h.i.=10, publications 200+
Scientific director
Nadezhda V. Rimazkaya Oleg S. Sutormin
Laboratory engineer
Laboratory engineer
Galina V. Zhukova
Laboratory engineer
Albert M. Oyun
CEO
Viktoria I.
Lonshakova-Mukina
Researcher
Prof. Peter I. Belobrov
Dr.Sc., h.i.=7, publications 120+
Scientific consultant
Kirill A. Lukyanenko
Researcher
Ivan A. Denisov
h.i.=1, publications 7
Lead and software engineer
Anton S. Yakimov
Design engineer
Vladimir V. Sorokin
Electronics engineer
11
Intellectual property
Patents:
Know-How:
1. Method of ''Enzimolyum'' fabrication
1. Unique collection of luminous bacteria
2. Water pollution bioassay
2. Extensive database of bioassays
3. Portable luminometer
3. Proprietary manufacturing process
Specifications:
1. Preparation of ''Enzimolyum'' #003534
4. Original software
5. Original signal amplifier
Trade Mark:
1. Trade mark Enzimolyum™
12
Sales plan
LumiShot ($850)
schools,
universities,
research laboratories
Сustomers
Sales
Profit
2015
$25k
$10k
2016–2017
$420k
$170k
2018–2020
$1.5m
$0.5m
13
Requested funding
Start of LumiShot mass production – 60 000 $
Third party services (5%)
Production (50%)
Expendable materials (5%)
Wages fund (40%)
14